CN1328316C - 生物活性降解膜的制备方法 - Google Patents
生物活性降解膜的制备方法 Download PDFInfo
- Publication number
- CN1328316C CN1328316C CNB2004100214740A CN200410021474A CN1328316C CN 1328316 C CN1328316 C CN 1328316C CN B2004100214740 A CNB2004100214740 A CN B2004100214740A CN 200410021474 A CN200410021474 A CN 200410021474A CN 1328316 C CN1328316 C CN 1328316C
- Authority
- CN
- China
- Prior art keywords
- solution
- dissolved
- polylactic acid
- bioactive
- fully
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
- 230000000975 bioactive effect Effects 0.000 title abstract 7
- 238000000034 method Methods 0.000 title description 2
- 239000000243 solution Substances 0.000 claims abstract description 40
- 229920001661 Chitosan Polymers 0.000 claims abstract description 12
- 229920000159 gelatin Polymers 0.000 claims abstract description 11
- 235000019322 gelatine Nutrition 0.000 claims abstract description 11
- 108010010803 Gelatin Proteins 0.000 claims abstract description 10
- 239000004372 Polyvinyl alcohol Substances 0.000 claims abstract description 10
- 239000008273 gelatin Substances 0.000 claims abstract description 10
- 235000011852 gelatine desserts Nutrition 0.000 claims abstract description 10
- 229920002451 polyvinyl alcohol Polymers 0.000 claims abstract description 10
- 238000002360 preparation method Methods 0.000 claims abstract description 10
- GHCZTIFQWKKGSB-UHFFFAOYSA-N 2-hydroxypropane-1,2,3-tricarboxylic acid;phosphoric acid Chemical compound OP(O)(O)=O.OC(=O)CC(O)(C(O)=O)CC(O)=O GHCZTIFQWKKGSB-UHFFFAOYSA-N 0.000 claims abstract description 5
- 230000003413 degradative effect Effects 0.000 claims description 10
- 239000012528 membrane Substances 0.000 claims description 10
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 9
- 230000004071 biological effect Effects 0.000 claims description 6
- 239000003855 balanced salt solution Substances 0.000 claims description 4
- 239000000872 buffer Substances 0.000 claims description 4
- 239000012153 distilled water Substances 0.000 claims description 4
- 239000005357 flat glass Substances 0.000 claims description 4
- 235000011194 food seasoning agent Nutrition 0.000 claims description 4
- 239000003292 glue Substances 0.000 claims description 4
- 229920000747 poly(lactic acid) Polymers 0.000 abstract description 9
- 230000008676 import Effects 0.000 abstract description 3
- 239000002994 raw material Substances 0.000 abstract description 3
- 239000004626 polylactic acid Substances 0.000 abstract 6
- 229920002101 Chitin Polymers 0.000 abstract 2
- 239000001828 Gelatine Substances 0.000 abstract 1
- 230000003139 buffering effect Effects 0.000 abstract 1
- 229920006237 degradable polymer Polymers 0.000 abstract 1
- 239000012154 double-distilled water Substances 0.000 abstract 1
- 239000011521 glass Substances 0.000 abstract 1
- 239000011259 mixed solution Substances 0.000 abstract 1
- 239000003814 drug Substances 0.000 description 10
- -1 poly(lactic acid) Polymers 0.000 description 6
- 239000000126 substance Substances 0.000 description 6
- 230000015556 catabolic process Effects 0.000 description 5
- 238000006731 degradation reaction Methods 0.000 description 5
- 229940079593 drug Drugs 0.000 description 4
- 238000001727 in vivo Methods 0.000 description 4
- 239000013543 active substance Substances 0.000 description 3
- 230000000694 effects Effects 0.000 description 3
- 230000004888 barrier function Effects 0.000 description 2
- 239000000463 material Substances 0.000 description 2
- 229920000642 polymer Polymers 0.000 description 2
- 150000005846 sugar alcohols Polymers 0.000 description 2
- 238000001356 surgical procedure Methods 0.000 description 2
- MSWZFWKMSRAUBD-IVMDWMLBSA-N 2-amino-2-deoxy-D-glucopyranose Chemical compound N[C@H]1C(O)O[C@H](CO)[C@@H](O)[C@@H]1O MSWZFWKMSRAUBD-IVMDWMLBSA-N 0.000 description 1
- 208000031737 Tissue Adhesions Diseases 0.000 description 1
- 230000003187 abdominal effect Effects 0.000 description 1
- 239000002253 acid Substances 0.000 description 1
- 230000009471 action Effects 0.000 description 1
- MSWZFWKMSRAUBD-UHFFFAOYSA-N beta-D-galactosamine Natural products NC1C(O)OC(CO)C(O)C1O MSWZFWKMSRAUBD-UHFFFAOYSA-N 0.000 description 1
- 229920002988 biodegradable polymer Polymers 0.000 description 1
- 239000004621 biodegradable polymer Substances 0.000 description 1
- 229940088623 biologically active substance Drugs 0.000 description 1
- 210000000988 bone and bone Anatomy 0.000 description 1
- 230000001684 chronic effect Effects 0.000 description 1
- 150000001875 compounds Chemical class 0.000 description 1
- 230000008473 connective tissue growth Effects 0.000 description 1
- 238000013270 controlled release Methods 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- 239000007857 degradation product Substances 0.000 description 1
- 238000001647 drug administration Methods 0.000 description 1
- 239000003937 drug carrier Substances 0.000 description 1
- 238000012377 drug delivery Methods 0.000 description 1
- 239000000835 fiber Substances 0.000 description 1
- 229960002442 glucosamine Drugs 0.000 description 1
- 238000002347 injection Methods 0.000 description 1
- 239000007924 injection Substances 0.000 description 1
- 239000003094 microcapsule Substances 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 231100000957 no side effect Toxicity 0.000 description 1
- 231100000252 nontoxic Toxicity 0.000 description 1
- 230000003000 nontoxic effect Effects 0.000 description 1
- 230000009965 odorless effect Effects 0.000 description 1
- 229920000728 polyester Polymers 0.000 description 1
- 230000002980 postoperative effect Effects 0.000 description 1
- 230000002265 prevention Effects 0.000 description 1
- 230000008439 repair process Effects 0.000 description 1
- 150000003384 small molecules Chemical class 0.000 description 1
- 238000013268 sustained release Methods 0.000 description 1
- 239000012730 sustained-release form Substances 0.000 description 1
- 210000001519 tissue Anatomy 0.000 description 1
- 230000017423 tissue regeneration Effects 0.000 description 1
Landscapes
- Medicinal Preparation (AREA)
- Materials For Medical Uses (AREA)
Abstract
本发明提供了一种生物活性降解膜的制备方法，所要解决的技术问题是：目前制备生物降解聚合物的原料常用的是聚乳酸和壳聚糖，聚乳酸合成的条件要求较高，多半采用进口的，价格昂贵；由聚乳酸制成的降解膜在体内降解时间很长，所以国内不常使用。本发明的要点是：明胶完全溶于PBS溶液中，配成0.3-5%溶液；壳聚糖完全溶于柠檬酸—磷酸缓冲液中，配成0.5-5%溶液；聚乙烯醇溶于双蒸水中，配成3-15%溶液，使其完全溶解呈无色透明的胶状；取明胶溶液、壳聚糖溶液、聚乙烯醇溶液，这三种溶液按4-8∶2-7∶3-7比例完全混合后，铺在玻璃板上，室温下自然干燥成膜。本发明的积极效果是：具有理想的缓释生物活性物质的作用，并在体内较快降解。
Description
技术领域
本发明涉及生物活性降解膜的制备方法
背景技术
生物降解聚合物是一种人工合成的有机物化合物，在体内可降解吸收、无毒、无蓄积，有较好的生物相容性，现已广泛应用于可吸收缝线，药物的缓释载体，接骨板和固定螺丝等。特别是作为药物缓释载体的研究为人关注。药物控制释放系统中生物降解高分子载体的作用，是使药物能以零级动力学速率或其它可控速率释放药物，随之药物释放的殆尽，高分子载体本身也就降解成小分子物质，并被人体所吸收或排除体外。通常用于药物控释的高分子载体主要聚酯类和一氨基酸类，主要是某些化学物质通过结构修饰或采用制剂学手段来控制药物释放的速率，延长药物作用时间。目前研究较多的是制成微球、微囊、中空纤维等形式，将药物包裹在其中，通过注射给药。制成降解膜的形式研究的不多。
制成生物降解膜主要分为二大类，一类是单独制成生物降解膜，不加入任何药物或活性物质，此膜可作为外科损伤组织间的机械屏障，预防腹部手术后组织粘连；也可作为膜引导组织再生技术在口腔颌面外科和骨科中应用，它创造一个屏障环境，防止结缔组织生长、引导骨再生，修复骨组织缺损。另一类是制备出含有药物或活性物质的降解膜，此类文献报道很少。
目前制备生物降解聚合物的原料常用的是聚乳酸和壳聚糖，聚乳酸合成的条件要求较高，多半采用进口的，价格昂贵；由聚乳酸制成的降解膜在体内降解时间很长，所以国内不常使用。
发明内容
本发明的目的在于提供一种含有生物活性降解膜的制备方法。用此方法制备出的生物活性降解膜可避免上述不足。
本发明的目的是这样实现的：以壳聚糖为主要原料，同时加入相关物质，制备出无色无味、柔软、半透明、具有一定的机械强度、一定弹性和韧性的膜状物。能够溶于水，但湿强度较好、抗拉性能好，便于手术中使用。最终降解产物为氨基葡萄糖是人体存在的物质，因而生物相容性好，无毒副作用。
本发明的制备方法是：
1、明胶完全溶于平衡盐溶液PBS溶液中，配成0.3-5％溶液.
2、壳聚糖完全溶于柠檬酸-磷酸缓冲液中，配成0.5-5％溶液.
3、聚乙烯醇溶于双蒸水中，配成3-15％溶液，使其完全溶解呈无色透明的胶状。
4、取明胶溶液、壳聚糖溶液、聚乙烯醇溶液，这三种溶液按4-8∶2-7∶3-7比例完全混合后，铺在玻璃板上，室温下自然干燥成膜。
本发明与现有技术相比，具有理想的缓释生物活性物质的作用，并在体内较快降解。具体特点是：
1、降解膜中含有生物活性物质，并保存其活性，是理想的载体。
2、生物降解膜非壳聚糖单一组分制成，加入相关物质使缓释速度加快，提高降解率。在体内降解时间为35天左右。
3、作为药物载体具有理想的缓释率。
4、保证该活性物质一定缓释速率的前提下，加入有关物质增加膜的韧性、强度和光泽，在水中湿强度较好，便于在手术中使用。
下面结合实施例进一步说明本发明。
具体实施方式
1、明胶完全溶于平衡盐溶液PBS溶液中，配成3％溶液.
2、壳聚糖完全溶于柠檬酸-磷酸缓冲液中，配成3％溶液.
3、聚乙烯醇溶于双蒸水中，配成10％溶液，使其完全溶解呈无色透明的胶状。
4、取明胶溶液、壳聚糖溶液、聚乙烯醇溶液，这三种溶液按6∶5∶5比例完全混合后，铺在玻璃板上，室温下自然干燥成膜。
Claims (2)
1、一种生物活性降解膜的制备方法，其特征是：
(1)、明胶完全溶于平衡盐溶液PBS溶液中，配成0.3-5％溶液；
(2)、壳聚糖完全溶于柠檬酸-磷酸缓冲液中，配成0.5-5％溶液；
(3)、聚乙烯醇溶于双蒸水中，配成3-15％溶液，使其完全溶解呈无色透明的胶状；
(4)、取明胶溶液、壳聚糖溶液、聚乙烯醇溶液，这三种溶液按4-8∶2-7∶3-7比例完全混合后，铺在玻璃板上，室温下自然干燥成膜。
2、按照权利要求1所述生物活性降解膜的制备方法，其特征是：
(1)、明胶完全溶于平衡盐溶液PBS溶液中，配成3％溶液；
(2)、壳聚糖完全溶于柠檬酸-磷酸缓冲液中，配成3％溶液；
(3)、聚乙烯醇溶于双蒸水中，配成10％溶液，使其完全溶解呈无色透明的胶状；
(4)、取明胶溶液、壳聚糖溶液、聚乙烯醇溶液，这三种溶液按6∶5∶5比例完全混合后，铺在玻璃板上，室温下自然干燥成膜。
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB2004100214740A CN1328316C (zh) | 2004-03-26 | 2004-03-26 | 生物活性降解膜的制备方法 |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB2004100214740A CN1328316C (zh) | 2004-03-26 | 2004-03-26 | 生物活性降解膜的制备方法 |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN1673273A CN1673273A (zh) | 2005-09-28 |
| CN1328316C true CN1328316C (zh) | 2007-07-25 |
Family
ID=35046060
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CNB2004100214740A Expired - Fee Related CN1328316C (zh) | 2004-03-26 | 2004-03-26 | 生物活性降解膜的制备方法 |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN1328316C (zh) |
Families Citing this family (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101372820B (zh) * | 2007-08-21 | 2010-11-17 | 马铁良 | 一种香烟自熄液及其制备方法 |
| CN102125811B (zh) * | 2011-01-10 | 2012-09-05 | 杭州水处理技术研究开发中心有限公司 | 一种聚乙烯醇-壳聚糖纳滤膜的制备方法 |
| CN102585264B (zh) * | 2012-01-20 | 2013-06-05 | 河南科技大学 | 一种用无机蒙脱土改良的明胶/pva复合膜的制备方法 |
| CN102585263B (zh) * | 2012-01-20 | 2013-06-05 | 河南科技大学 | 一种用有机蒙脱土改良的明胶/pva复合膜的制备方法 |
| CN115895053B (zh) * | 2022-08-04 | 2024-03-26 | 盐城工学院 | 一种生物可降解膜及其制备方法和应用 |
Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS529070A (en) * | 1975-07-11 | 1977-01-24 | Mitsubishi Monsanto Chem | Process for molding polyvinyl chloride foam |
| CN1063425A (zh) * | 1992-01-17 | 1992-08-12 | 厦门大学 | 高效分离膜的制作法 |
| CN1111652A (zh) * | 1994-05-10 | 1995-11-15 | 池洪 | 可生物降解无污发泡水基材料 |
| CN1112024A (zh) * | 1995-05-01 | 1995-11-22 | 付朝宏 | 一种医用生物薄膜的制备方法 |
| KR20010016482A (ko) * | 2000-12-14 | 2001-03-05 | 손태원 | 키토산과 젤라틴으로 조성된 연질 겔 |
| CN1296852A (zh) * | 2000-12-12 | 2001-05-30 | 天津大学 | 脂肪族聚酯表面的壳聚糖/明胶网络修饰 |
| CN1411805A (zh) * | 2001-10-11 | 2003-04-23 | 北京中科天鹤生物技术有限公司 | 一种生物药膜及其制备方法 |
-
2004
- 2004-03-26 CN CNB2004100214740A patent/CN1328316C/zh not_active Expired - Fee Related
Patent Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS529070A (en) * | 1975-07-11 | 1977-01-24 | Mitsubishi Monsanto Chem | Process for molding polyvinyl chloride foam |
| CN1063425A (zh) * | 1992-01-17 | 1992-08-12 | 厦门大学 | 高效分离膜的制作法 |
| CN1111652A (zh) * | 1994-05-10 | 1995-11-15 | 池洪 | 可生物降解无污发泡水基材料 |
| CN1112024A (zh) * | 1995-05-01 | 1995-11-22 | 付朝宏 | 一种医用生物薄膜的制备方法 |
| CN1296852A (zh) * | 2000-12-12 | 2001-05-30 | 天津大学 | 脂肪族聚酯表面的壳聚糖/明胶网络修饰 |
| KR20010016482A (ko) * | 2000-12-14 | 2001-03-05 | 손태원 | 키토산과 젤라틴으로 조성된 연질 겔 |
| CN1411805A (zh) * | 2001-10-11 | 2003-04-23 | 北京中科天鹤生物技术有限公司 | 一种生物药膜及其制备方法 |
Non-Patent Citations (4)
| Title |
|---|
| 壳聚糖一明胶共混膜 余家会等，武汉大学学报 自然科学版，第45卷第4期 1999 * |
| 壳聚糖水杨酸盐一明胶共混膜结构表征及其抗菌性 赵铁等，分析科学学报，第18卷第2期 2002 * |
| 壳聚糖水杨酸盐一明胶共混膜结构表征及其抗菌性 赵铁等，分析科学学报，第18卷第2期 2002;壳聚糖一明胶共混膜 余家会等，武汉大学学报 自然科学版，第45卷第4期 1999;生物可降解包装膜 陈洁等，无锡轻工大学学报，第19卷第4期 2000 * |
| 生物可降解包装膜 陈洁等，无锡轻工大学学报，第19卷第4期 2000 * |
Also Published As
| Publication number | Publication date |
|---|---|
| CN1673273A (zh) | 2005-09-28 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| DeStefano et al. | Applications of PLA in modern medicine | |
| Ginjupalli et al. | Poly (α-hydroxy acid) based polymers: A review on material and degradation aspects | |
| Islam et al. | Chitin and chitosan: structure, properties and applications in biomedical engineering | |
| Jin et al. | Electrospun silver ion-loaded calcium phosphate/chitosan antibacterial composite fibrous membranes for guided bone regeneration | |
| Perez et al. | Polymeric additives to enhance the functional properties of calcium phosphate cements | |
| Hussain et al. | A review on PLA-based biodegradable materials for biomedical applications | |
| CA2677498C (en) | Polymer formulations for delivery of bioactive agents | |
| CN100569299C (zh) | 可降解复合生物材料医用膜 | |
| Sharma et al. | Natural biodegradable polymers as matrices in transdermal drug delivery | |
| CN102770164B (zh) | 减少医疗装置和植入物上微生物附着的可吸收聚乙烯二乙醇酸酯共聚物 | |
| Mtibe et al. | Recent insight into the biomedical applications of polybutylene succinate and polybutylene succinate-based materials | |
| Ito et al. | Preparation of injectable auto-forming alginate gel containing simvastatin with amorphous calcium phosphate as a controlled release medium and their therapeutic effect in osteoporosis model rat | |
| CN101623517A (zh) | 一种医用防粘连膜及其制备方法 | |
| Vacaras et al. | Understanding the basis of medical use of poly-lactide-based resorbable polymers and composites–a review of the clinical and metabolic impact | |
| Huang et al. | Preparation of novel stable microbicidal hydrogel films as potential wound dressing | |
| Sadeghianmaryan et al. | Advancements in 3D-printable polysaccharides, proteins, and synthetic polymers for wound dressing and skin scaffolding–a review | |
| Abdelhak | A review: Application of biopolymers in the pharmaceutical formulation | |
| CN1328316C (zh) | 生物活性降解膜的制备方法 | |
| Sharma et al. | An overview: non-toxic and eco-friendly polysaccharides—its classification, properties, and diverse applications | |
| Joseph et al. | Advances in biopolymer based surgical sutures | |
| Broda et al. | Biodegradable polymers in veterinary medicine—A review | |
| Faikrua et al. | Properties of β-glycerol phosphate/collagen/chitosan blend scaffolds for application in skin tissue engineering | |
| CN104873535A (zh) | 一种聚乳酸-羟基乙酸共聚物防粘连液及其制备方法 | |
| Lopes et al. | Renewable Polymers in Biomedical Applications: From the Bench to the Market | |
| Hua et al. | Anti-tuberculosis drug delivery for tuberculous bone defects |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| C19 | Lapse of patent right due to non-payment of the annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |