CN1282511A - Method for fixating shifting sand with algal eremophyte - Google Patents
Method for fixating shifting sand with algal eremophyte Download PDFInfo
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- CN1282511A CN1282511A CN 99116583 CN99116583A CN1282511A CN 1282511 A CN1282511 A CN 1282511A CN 99116583 CN99116583 CN 99116583 CN 99116583 A CN99116583 A CN 99116583A CN 1282511 A CN1282511 A CN 1282511A
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- algae
- algae species
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- desert
- quicksand
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- 238000000034 method Methods 0.000 title claims abstract description 14
- 239000004576 sand Substances 0.000 title abstract description 18
- 241000195493 Cryptophyta Species 0.000 claims abstract description 118
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 21
- 238000011081 inoculation Methods 0.000 claims abstract description 15
- 239000007788 liquid Substances 0.000 claims abstract description 8
- 239000002689 soil Substances 0.000 claims abstract description 8
- 229920001817 Agar Polymers 0.000 claims abstract description 5
- 239000008272 agar Substances 0.000 claims abstract description 5
- 238000012216 screening Methods 0.000 claims abstract description 5
- 239000008223 sterile water Substances 0.000 claims abstract description 4
- 238000005119 centrifugation Methods 0.000 claims abstract 2
- 230000003628 erosive effect Effects 0.000 claims abstract 2
- 239000003337 fertilizer Substances 0.000 claims description 10
- 238000004321 preservation Methods 0.000 claims description 5
- 238000000926 separation method Methods 0.000 claims description 5
- 238000003973 irrigation Methods 0.000 claims description 4
- 230000002262 irrigation Effects 0.000 claims description 4
- 150000001875 compounds Chemical class 0.000 claims 3
- 244000025254 Cannabis sativa Species 0.000 claims 2
- 241000483023 Cryphia algae Species 0.000 claims 1
- 238000012258 culturing Methods 0.000 claims 1
- 238000001914 filtration Methods 0.000 claims 1
- 238000000227 grinding Methods 0.000 claims 1
- 239000002985 plastic film Substances 0.000 claims 1
- 229920006255 plastic film Polymers 0.000 claims 1
- 238000000746 purification Methods 0.000 claims 1
- 238000002791 soaking Methods 0.000 claims 1
- 206010039509 Scab Diseases 0.000 abstract 1
- 239000011521 glass Substances 0.000 abstract 1
- 241000848519 Scytonema arcangelii Species 0.000 description 7
- 241000196324 Embryophyta Species 0.000 description 6
- 239000011324 bead Substances 0.000 description 6
- 239000002131 composite material Substances 0.000 description 6
- 239000002609 medium Substances 0.000 description 6
- 235000015097 nutrients Nutrition 0.000 description 6
- 238000011160 research Methods 0.000 description 5
- 241000195649 Chlorella <Chlorellales> Species 0.000 description 4
- 230000004888 barrier function Effects 0.000 description 4
- 230000000694 effects Effects 0.000 description 4
- 239000010902 straw Substances 0.000 description 4
- 230000003442 weekly effect Effects 0.000 description 4
- 241000180279 Chlorococcum Species 0.000 description 3
- 238000001035 drying Methods 0.000 description 3
- 239000007787 solid Substances 0.000 description 3
- 235000013311 vegetables Nutrition 0.000 description 3
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 2
- 241000192700 Cyanobacteria Species 0.000 description 2
- 238000007710 freezing Methods 0.000 description 2
- 230000008014 freezing Effects 0.000 description 2
- 239000001963 growth medium Substances 0.000 description 2
- 238000009434 installation Methods 0.000 description 2
- 230000002045 lasting effect Effects 0.000 description 2
- 230000035479 physiological effects, processes and functions Effects 0.000 description 2
- 230000001954 sterilising effect Effects 0.000 description 2
- 238000004659 sterilization and disinfection Methods 0.000 description 2
- 239000003643 water by type Substances 0.000 description 2
- 230000003044 adaptive effect Effects 0.000 description 1
- 230000032683 aging Effects 0.000 description 1
- 238000013459 approach Methods 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 239000012141 concentrate Substances 0.000 description 1
- 238000005260 corrosion Methods 0.000 description 1
- 230000007797 corrosion Effects 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 230000018109 developmental process Effects 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 239000000834 fixative Substances 0.000 description 1
- 229910052757 nitrogen Inorganic materials 0.000 description 1
- 230000002035 prolonged effect Effects 0.000 description 1
- 230000005855 radiation Effects 0.000 description 1
- 239000002688 soil aggregate Substances 0.000 description 1
- 238000004162 soil erosion Methods 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 230000001228 trophic effect Effects 0.000 description 1
- 238000005406 washing Methods 0.000 description 1
- 239000002699 waste material Substances 0.000 description 1
Landscapes
- Cultivation Of Plants (AREA)
Abstract
本发明公开了一种荒漠藻对流沙的固定方法,首先是藻种分离,将干旱、半干旱荒漠土表结皮研磨,无菌水浸泡后培养;其次是藻种纯化,将培养的藻种,用无菌接种棒、解剖针将藻种挑出,在无菌载玻片上灭菌,接入琼脂平板培养皿;第三是藻种培养,将分离,纯化的藻类接入液体培养基,在一定条件下,通气培养;第四是藻种筛选,将培养藻种接入室内及室外流沙,选择速度快、抗风蚀强的藻种与藻种搭配;第五是藻种保存,将所筛选的藻种培养后,离心收集,涂抹于丝绢之上,阴干、粉碎、过筛、干燥保存;第六是野外接种。本发明方法易行,能快速、高效、持久的固沙。The invention discloses a method for fixing desert algae to quicksand. Firstly, the algae species are separated, and the surface crusts of arid and semi-arid desert soil are ground, soaked in sterile water, and cultivated; secondly, the algae species are purified, and the cultured algae species are purified. , pick out the algae species with sterile inoculation sticks and dissecting needles, sterilize them on sterile glass slides, and insert them into agar plate culture dishes; the third is the cultivation of algae species, inserting the separated and purified algae into liquid medium, Under certain conditions, aerated culture; the fourth is the screening of algae species, which is to insert the cultivated algae species into the indoor and outdoor quicksand, and select the algae species with fast speed and strong wind erosion resistance to match with the algae species; the fifth is to preserve the algae species After the selected algae are cultured, they are collected by centrifugation, smeared on silk, dried in the shade, crushed, sieved, and stored in a dry place; the sixth is field inoculation. The method of the invention is easy to implement, and can fast, efficiently and durable sand fixation.
Description
The present invention relates to the fixing means of a kind of desert algae, be applicable to quick, the extended immobilization of Desert Area drift sand drift sand.
Increasingly serious along with the desertification situation, national governments, each subject expert are taking a lot of trouble and efforts for seeking better control measures, no matter machinery, chemistry, still the several different methods of biology is used, but mechanical dune work amount is heavy, effect is not good enough; There are problems such as fixative is aging, the soil can't utilize again in chemical fixation; In conjunction with the biological comprehensive regulation originally is the approach that is hopeful fundamentally to administer the desert most, changes environment, but at present this field only limit to high anti-morning of plant introduction and acclimatization, optimize and select and aspects such as reasonable disposition shelter forest.And plant seedlings needs several years even more than ten years could really play windproof fixing effect, and therefore, although the undershrub growth is arranged, it is mobile that Shamian Island remained in the quite a while.The primary symbol of actual desert real fixed is to form the algae skinning on the Shamian Island, and the existence of this structure can make soil erosion amount be reduced to zero.Therefore, the research of desert algae is very active in the world wide, many algologists have successively carried out research and trial extensively and profoundly at many levels from many-sides such as the taxonomy of desert algae, ecology, physiology, biochemistry, molecular biology, but corrosion resistance research still concentrates on the influence aspect of weathering to soil aggregate quantity, stability, research about desert algae, the weather-proof aspect of desert algae skinning, behind Booth (1941), Loope﹠amp; Gifford (1972), Dong Guangrong, Li Changzhi etc. (1987), Hu Mengchun, Liu Yuzhang etc. (1991) have done further research, but all be confined to the influence of external conditions such as the having or not of algae skinning, destructiveness, water content height, wind behaviour and vegetation coverage, the most critical biology-desert algae that forms from the algae skinning is not started with, thereby can't accomplish artificial culture and regulation and control.
Utilize the desert algae solid cubic metre of earth face of fostering and apply fertilizer not have patent documentation so far.
The purpose of this invention is to provide the fixing means of a kind of desert algae to drift sand, fixedly the algae kind is easy to implement the method, and what energy was quick, efficient, lasting fixes.
To achieve these goals, its main technical schemes is: from local desert environment separation, purifying, filter out and fix the sand the algae kind preferably, and carry out batch and cultivate, select actual available supplementary measures, carry out open-air drift sand inoculation.Its step is as follows:
1, the separation of algae kind.Arid, semiarid zone desert soil table skinning are ground, after sterile water soaks certain hour, behind the access solid culture medium certain hour, choose frond, microscopically is observed, identifies, is cultivated separately.
2, the purifying of algae kind.With the algae kind of cultivating separately, or the culture dish after finishing the algae kind and identifying, putting binocular body formula microscopically observes, with aseptic inoculation rod, dissecting needle, capillary and ophthalmologic operation pincet required algae kind is chosen, under aseptic slide, washed in several sterilization Chu ' s10 solution one by one, inserted new agar plate culture dish then, cultivated until obtaining single algae.
3, the cultivation of algae kind.The algae of separation, purifying is inserted HGZ, BG11 or HB-D1 liquid nutrient medium, fixed nitrogen blue-green algae such as single qi algae as the algae that quivers, little sheath algae, seat algae.Scytonema arcangelii, beads algae equal the BG110 liquid nutrient medium, behind the aerobic culture, green alga such as Protococcus viridis chlorella etc. are inserted BBM, HB-D1 or Zhu Shi liquid nutrient medium, aerobic culture under certain condition.
4, the screening of algae kind.To cultivate the algae kind in batches and insert indoor and outdoor drift sand, measure growth rate and weather-proof intensity, selection fast growth, algae kind and the collocation of algae kind that weather-proof ability is strong.
5, the preservation of algae kind.After institute screening algae kind cultivated in batches, centrifugal or filter and collect, evenly be applied in silk beautiful on, dry in the shade, weigh, pulverize, sieve, drying preservation, but also freezing of blue-green algae.
6, field inoculation.
1) in the inoculation place, sets up movable sprinkler irrigation or other short-term water supply installation.
2) drift sand is separated in plastic mulching, inserts effectively to fix the sand the algae kind in right amount, takes off film every morning, afternoon press mold; Execute agricultural composite fertilizer weekly once, behind the certain hour, cut off the water, remove water supply plant, this method can adopt at spring, summer, Qiu Sanji.
3) bare area is pricked and is established straw checkerboard barrier, and an amount of inoculation is watered, and executes agricultural composite fertilizer weekly once, behind the certain hour, cuts off the water, and removes water supply plant.Be suitable for spring, summer, Qiu Sanji.
4) directly insert selected algae kind in straw checkerboard barrier or the bulk drift sand, need not water, the rainy day is executed agricultural composite fertilizer, is suitable for summer, and particularly 5,6, July.
The result who obtains
1) separate and purifying 15 algae kinds, promptly anabena, sheath silk algae, the algae that quivers, a kind of little sheath algae are split an algae, deliver vegetables a kind of beads algae, seat algae, Dan Qizao, Scytonema arcangelii, cytoalgae, chlorella, Chlorococcum, collection ball algae, Protococcus viridis.
2) in batches cultivated wherein 12 kinds, i.e. sheath silk algae, Dan Qizao, the algae that quivers is split an algae, and a kind of little sheath algae is delivered vegetables, beads algae, seat algae, Scytonema arcangelii, chlorella, Chlorococcum, Protococcus viridis.
3) screen a kind of little sheath algae, Dan Qizao, Scytonema arcangelii three single kinds and little sheath algae account for 60%, Scytonema arcangelii, the beads algae, Dan Qizao, the seat algae, Protococcus viridis respectively accounts for 8% best algae kind combination.
4) obtain the method for algae kind drying or freezing.
5) obtain sandstorm level algae skinning after 20-40 days.
The present invention compared with prior art, have the following advantages and effect: easy to implement the method, quick, efficient, lasting the fixing the sand of energy, this method is suitable for the raising of progressively improvement, soil structure improved addictive and trophic level of fixing, the desert environment of drift sand in the whole desert environment, and the improvement to arid, semiarid zone economic development people's lives all has excellent application value, and its major advantage has:
1) desert algae can be with the fastest speed with the Shamian Island real fixed, and in the keeping of the early stage fixing and algae skinning intensity in desert, can not be substituted play most important effect.
2) desert algae is as planting a colony biology, to environmental condition require lowly, the cyclostage is short, the heredity of kind is selected and improvement is fast and easily.
3) desert algae is all having a series of adaptive strategies to barren, the prolonged drought of desert environment, high radiation, windy and dusty and do-wet fast, freeze-molten variation aspect Physiology and biochemistry and the ecological niche.
4) realization of formation of the short-term of sandstorm level algae skinning and intensity has confirmed that algae is fixed with very big feasibility.
5) desert algae is having permanent effective function aspect soil melioration and the anti-erosion, therefore once drops into, permanent being benefited.
6) g and D of the economic algae kind of many waste algaes (as deliver vegetables etc.) illustrates that the fixing and planting technology of little algae not only can tackling quicksand, also can help the poverty-stricken area to shake off poverty and set out on the road to prosperity, so this technology has very tempting application prospect.
Embodiment:
1, the separation of algae kind.Shapotou desert soil skinning is ground the 0.01mm soil sieve, after sterile water soaks 24h, inserted No. 10, Zhu Shi, BBM, BG11 and BG110 solid culture medium (1.2-1.5% agar), half puts 60-70 μ E m
-2s
-1, 19-21 ℃ cultivates down, second half puts 70-100 μ E m
-2s
-1, cultivate under the 29-31 ℃ condition, frond is chosen in 14-16 and 29-31 days, microscopically is observed, identifies, is cultivated separately.
2, the purifying of algae kind.With the algae kind of cultivating separately, or the culture dish after finishing the algae kind and identifying, putting binocular body formula microscopically observes, with aseptic inoculation rod, dissecting needle, capillary and ophthalmologic operation pincet required algae kind is chosen, in several sterilization Chu ' s10 solution, washing one by one on the aseptic slide, insert the agar plate culture dish then, so separate, move and connect, cultivate until obtaining single algae.
3, the cultivation of algae kind.With the pure strain algae that quivers, little sheath algae and sheath silk algae are inserted BG11 or HB-D
1Liquid nutrient medium, Dan Qizao, a kind of beads algae, anabena is put 60-70 μ E m in the BG110 liquid nutrient medium
-2s
-1, 29-31 ℃ of following aerobic culture, Chlorococcum and chlorella are at BBM or BG11 liquid nutrient medium, in 70-100 μ Em
-2s
-1, aerobic culture under the 19-21 ℃ of condition.
4, algae kind screening.To cultivate the algae kind in batches with 0.003mg/cm
2Amount inserts indoor and outdoor drift sand, mensuration by growth rate and weather-proof intensity, select strong algae kind of fast growth, weather-proof ability and the collocation of algae kind, be little sheath algae, the seat algae, Scytonema arcangelii and little sheath algae account for 60%, the seat algae accounts for 8%, Scytonema arcangelii accounts for 8%, and the beads algae accounts for 8%, and Protococcus viridis accounts for 8% combination.
5, the preservation of algae kind.To screen after the algae kind cultivates in batches, centrifugal (1000 change, and 5min), or filter and collect, evenly be applied in silk beautiful on, dry in the shade, weigh, pulverize, sieve (0.01mm), drying preservation.
6, field inoculation.
1) in the inoculation place, sets up movable sprinkler irrigation or other short-term water supply installation.
2) drift sand is separated in plastic mulching, and is economical, insert and fix the sand ability algae kind in right amount effectively, and every morning, 8:00-9:00 took off film, afternoon the 17:30-18:00 press mold; 10:00-12:00,14:30-16:30 waters, unit interval water consumption 0.00198ml/cm
2Min executes agricultural composite fertilizer weekly once, each 15.68g/m
2, after 30 days, cut off the water, remove water supply plant.This method can adopt at spring, summer, Qiu Sanji.
3) bare area is pricked and is established straw checkerboard barrier (1.5 * 15m
2), suitable inoculation, 10:00-12:00,14:30-1630 waters, unit interval water consumption 0.00198ml/cm
2Min executes agricultural composite fertilizer weekly once, each 1568g/m
2, after 30 days, cut off the water, remove water supply plant.This method can adopt at spring, summer, Qiu Sanji.
4) directly insert selected algae kind in straw checkerboard barrier or the bulk drift sand, need not water, the rainy day is executed agricultural composite fertilizer, each 24.66g/m
2This method is applicable to summer, particularly 5,6, and July.
Claims (2)
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB991165837A CN1140167C (en) | 1999-08-03 | 1999-08-03 | Fixation method of desert algae to quicksand |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB991165837A CN1140167C (en) | 1999-08-03 | 1999-08-03 | Fixation method of desert algae to quicksand |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN1282511A true CN1282511A (en) | 2001-02-07 |
| CN1140167C CN1140167C (en) | 2004-03-03 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CNB991165837A Expired - Fee Related CN1140167C (en) | 1999-08-03 | 1999-08-03 | Fixation method of desert algae to quicksand |
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Cited By (14)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN100383345C (en) * | 2005-08-18 | 2008-04-23 | 中国科学院新疆生态与地理研究所 | Sand-fixing method of terrestrial nitrogen-fixing cyanobacteria |
| CN100421548C (en) * | 2004-03-27 | 2008-10-01 | 中国科学院水生生物研究所 | A Composite Method for Biological Fixation of Quicksand and Restoration of Desertified Land |
| CN102199542A (en) * | 2011-05-06 | 2011-09-28 | 中国科学院新疆生态与地理研究所 | Method for purifying and separating Microcoleus vaginatus |
| CN102612953A (en) * | 2012-03-28 | 2012-08-01 | 中国科学院新疆生态与地理研究所 | Pattern for building artificial algal crust in desert |
| CN102757792A (en) * | 2012-06-28 | 2012-10-31 | 环境保护部华南环境科学研究所 | Compound ecological stand stabilizing agent prepared from sewage sludge as well as preparation method and application of compound ecological stand stabilizing agent |
| CN103153495A (en) * | 2010-07-15 | 2013-06-12 | 国立顺天大学校产学协力团 | Method for producing thalli of lichens, method for restoring the degraded ecology by them, and compositions therefor |
| CN106638339A (en) * | 2017-01-18 | 2017-05-10 | 甘肃省治沙研究所 | Algae blanket and preparing method thereof |
| CN106754390A (en) * | 2016-12-30 | 2017-05-31 | 山东宝来利来生物工程股份有限公司 | The albuminiferous chlorella of one plant height and its cultural method and application |
| CN107012098A (en) * | 2017-03-19 | 2017-08-04 | 西北师范大学 | A kind of preparation method of loess desert algae composite sand consolidation material |
| CN107996054A (en) * | 2017-11-13 | 2018-05-08 | 中国矿业大学 | A kind of artificial algae skinning method suitable for arid and semi-arid area plant on sand land |
| CN108396001A (en) * | 2018-02-14 | 2018-08-14 | 新疆隆博叶希丽生态环保有限公司 | Desert algae mixed liquor and the method for application and sand control with biological measures |
| CN109305828A (en) * | 2018-10-17 | 2019-02-05 | 雷云飞 | A kind of microalgae nutrition reparation liquid and preparation method thereof for alleviating Steppe Desertification |
| CN111492744A (en) * | 2020-04-29 | 2020-08-07 | 江西离子型稀土工程技术研究有限公司 | Rapid cultivation method for forming biological soil crust by using ionic rare earth waste mine soil and application of rapid cultivation method |
| CN118930357A (en) * | 2024-07-08 | 2024-11-12 | 扬州大学 | A method for enhancing the sand-fixing effect of slow-release fertilizer using algae |
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| CN100370005C (en) * | 2005-01-13 | 2008-02-20 | 中国科学院水生生物研究所 | Improvement method of desert and semi-desert soil by soil algae |
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1999
- 1999-08-03 CN CNB991165837A patent/CN1140167C/en not_active Expired - Fee Related
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| CN100421548C (en) * | 2004-03-27 | 2008-10-01 | 中国科学院水生生物研究所 | A Composite Method for Biological Fixation of Quicksand and Restoration of Desertified Land |
| CN100383345C (en) * | 2005-08-18 | 2008-04-23 | 中国科学院新疆生态与地理研究所 | Sand-fixing method of terrestrial nitrogen-fixing cyanobacteria |
| CN103153495A (en) * | 2010-07-15 | 2013-06-12 | 国立顺天大学校产学协力团 | Method for producing thalli of lichens, method for restoring the degraded ecology by them, and compositions therefor |
| CN102199542A (en) * | 2011-05-06 | 2011-09-28 | 中国科学院新疆生态与地理研究所 | Method for purifying and separating Microcoleus vaginatus |
| CN102199542B (en) * | 2011-05-06 | 2012-09-19 | 中国科学院新疆生态与地理研究所 | A kind of method for purifying and separating Microcoletheca |
| CN102612953A (en) * | 2012-03-28 | 2012-08-01 | 中国科学院新疆生态与地理研究所 | Pattern for building artificial algal crust in desert |
| CN102757792A (en) * | 2012-06-28 | 2012-10-31 | 环境保护部华南环境科学研究所 | Compound ecological stand stabilizing agent prepared from sewage sludge as well as preparation method and application of compound ecological stand stabilizing agent |
| CN106754390A (en) * | 2016-12-30 | 2017-05-31 | 山东宝来利来生物工程股份有限公司 | The albuminiferous chlorella of one plant height and its cultural method and application |
| CN106754390B (en) * | 2016-12-30 | 2019-07-26 | 山东宝来利来生物工程股份有限公司 | The albuminiferous chlorella of one plant height and its cultural method and application |
| CN106638339A (en) * | 2017-01-18 | 2017-05-10 | 甘肃省治沙研究所 | Algae blanket and preparing method thereof |
| CN106638339B (en) * | 2017-01-18 | 2022-04-08 | 甘肃省治沙研究所 | Algae blanket and preparation method thereof |
| CN107012098A (en) * | 2017-03-19 | 2017-08-04 | 西北师范大学 | A kind of preparation method of loess desert algae composite sand consolidation material |
| CN107996054A (en) * | 2017-11-13 | 2018-05-08 | 中国矿业大学 | A kind of artificial algae skinning method suitable for arid and semi-arid area plant on sand land |
| CN108396001A (en) * | 2018-02-14 | 2018-08-14 | 新疆隆博叶希丽生态环保有限公司 | Desert algae mixed liquor and the method for application and sand control with biological measures |
| CN108396001B (en) * | 2018-02-14 | 2019-03-01 | 新疆隆博叶希丽生态环保有限公司 | Desert algae mixed liquor and the method for application and sand control with biological measures |
| CN109305828A (en) * | 2018-10-17 | 2019-02-05 | 雷云飞 | A kind of microalgae nutrition reparation liquid and preparation method thereof for alleviating Steppe Desertification |
| CN111492744A (en) * | 2020-04-29 | 2020-08-07 | 江西离子型稀土工程技术研究有限公司 | Rapid cultivation method for forming biological soil crust by using ionic rare earth waste mine soil and application of rapid cultivation method |
| CN118930357A (en) * | 2024-07-08 | 2024-11-12 | 扬州大学 | A method for enhancing the sand-fixing effect of slow-release fertilizer using algae |
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| Publication number | Publication date |
|---|---|
| CN1140167C (en) | 2004-03-03 |
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