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CN106638339B - Algae blanket and preparation method thereof - Google Patents

Algae blanket and preparation method thereof Download PDF

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CN106638339B
CN106638339B CN201710034498.7A CN201710034498A CN106638339B CN 106638339 B CN106638339 B CN 106638339B CN 201710034498 A CN201710034498 A CN 201710034498A CN 106638339 B CN106638339 B CN 106638339B
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刘淑娟
袁宏波
刘虎俊
万翔
李菁菁
刘开琳
李学敏
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Abstract

本发明涉及一种环保领域,尤其涉及一种藻毯及其制备方法。一种藻毯,从上到下依次包括上层保护纤维织物层、藻种层、基质层、定型支撑网和底层粘结带,上层保护纤维织物层、定型支撑网、底层粘结带采用缝编线缝接或用改性淀粉胶粘接,基质层与定型支撑网粘接,藻种层与基质层粘接,底层粘结带为纤维织物,其两侧分别比定型支撑网的两侧宽2cm,作为藻毯相互粘接的粘接带。一种藻毯的制备方法,包括以下步骤:(1)制备营养液;(2)制备基质;(3)制备荒漠藻;(4)制备藻毯。

Figure 201710034498

The invention relates to the field of environmental protection, in particular to an algal blanket and a preparation method thereof. An algal blanket, from top to bottom, comprises an upper protective fiber fabric layer, an algae seed layer, a matrix layer, a shaping support net and a bottom adhesive tape, and the upper protective fiber fabric layer, the shaping support mesh, and the bottom adhesive tape are stitched. Thread stitched or bonded with modified starch glue, the matrix layer is bonded with the shaping support net, the algae seed layer is bonded with the matrix layer, and the bottom bonding tape is a fiber fabric, the two sides of which are respectively wider than the two sides of the shaping support net 2cm, as the adhesive tape for bonding the algal blankets to each other. A method for preparing an algal blanket, comprising the following steps: (1) preparing a nutrient solution; (2) preparing a substrate; (3) preparing a desert algae; (4) preparing an algal blanket.

Figure 201710034498

Description

一种藻毯及其制备方法A kind of algal blanket and preparation method thereof

技术领域technical field

本发明涉及一种环保领域,尤其涉及一种藻毯及其制备方法。The invention relates to the field of environmental protection, in particular to an algal blanket and a preparation method thereof.

背景技术Background technique

随着荒漠化扩展,以及交通、开矿和建筑等工程形成的大量裸地出现,各国政府和各学科专家都在寻求较好的治理流沙地、水土流失地、劣地和裸地的措施。With the expansion of desertification and the emergence of a large number of bare lands formed by transportation, mining and construction projects, governments and experts from various disciplines are seeking better measures to control quicksand, soil erosion, poor land and bare land.

大量的工程的、化学的、生物和综合措施已被采用,但工程方法存在时效性,化学试剂存在土地再利用困难等问题。A large number of engineering, chemical, biological and comprehensive measures have been adopted, but the engineering methods are time-sensitive, and the chemical reagents are difficult to reuse the land.

生物综合治理本是最长效的治理荒漠化、改善环境的途径,但目前这一领域仅限于高等植物的优化选择和合理配置。特别是在200mm以下的荒漠地带,高密度的大面积造林,虽然生态环境短期改善,但因缺水导致人工林很快衰退,造成了生态进一步退化和资源的浪费。水分承载力是不同生态条件下植被类型存在的关键,而微生物结皮需要的水分相对较少,可达到全面覆盖,可使土壤侵蚀量降低到零。荒漠地带由维管束植物组成的植被盖度≤30%(Townshend等,1986),而微型生物结皮的盖度≥70%(Belnap等,1994)。因此,世界范围内的荒漠区微生物结皮研究备受关注,从微生物结皮的组成、结构演替、作用等多方面多层次进行了广泛深入的研究,并将其尝试应用于流沙固定等方面。魏江春院士提出了沙漠生物地毯工程,胡春香等提出了“荒漠藻对流沙的固定方法”(CN1282511A):是将筛选的藻种直接喷于流沙,制造人工结皮。刘永定等提出的“一种生物固定流沙和修复荒漠化土地的方法(CN1561698A)”,将荒漠藻与草本和灌木结合起来固定流沙。上述微生物结皮研究与应用只限于结皮自身及其微生物的直接应用。而无论是流沙地还是工程裸地,其物理和化学性质对生物生存都是严酷的,将微生物直接喷或撒到地表,生长比较困难。如果将荒漠藻与其生长基质结合,形成人工荒漠藻生长系统,让荒漠藻在适应的基质中生长,形成生长微环境,通过人工调控荒漠藻生长基质,增强荒漠藻存活、扩繁和应用能力,提高荒漠藻治理生态环境的效率。目前也有植被毯和生物毯应用于生态建设,但这种生物毯都是以草本植物为主体,利用藻类做成的人工生物结皮——藻毯至今没有专利文献。Biological integrated management is the most effective way to control desertification and improve the environment, but at present this field is limited to the optimal selection and rational allocation of higher plants. Especially in the desert area below 200mm, high-density large-scale afforestation, although the ecological environment is improved for a short time, but the plantation quickly declines due to lack of water, resulting in further ecological degradation and waste of resources. Water carrying capacity is the key to the existence of vegetation types under different ecological conditions, and microbial crusts require relatively little water, which can achieve full coverage and reduce soil erosion to zero. The vegetation coverage of desert areas is composed of vascular plants ≤30% (Townshend et al., 1986), while the coverage of microbiological crusts is ≥70% (Belnap et al., 1994). Therefore, the research on microbial crusts in desert areas around the world has attracted much attention, and extensive and in-depth research has been carried out on the composition, structural succession, and function of microbial crusts at various levels, and it has been tried to be applied to quicksand fixation and other aspects. . Academician Wei Jiangchun proposed the desert biological carpet project, and Hu Chunxiang et al. proposed the "fixation method of desert algae to quicksand" (CN1282511A): spraying the screened algal species directly on the quicksand to create artificial crusts. "A method of biological immobilization of quicksand and restoration of desertified land (CN1561698A)" proposed by Liu Yongding et al. combines desert algae with herbs and shrubs to immobilize quicksand. The research and application of the above-mentioned microbial crusts are limited to the direct application of the crusts themselves and their microorganisms. Whether it is quicksand or engineering bare land, its physical and chemical properties are harsh for biological survival, and it is difficult to grow microorganisms by spraying or spreading them directly on the surface. If the desert algae is combined with its growth substrate to form an artificial desert algae growth system, the desert algae can grow in the adapted substrate to form a growth microenvironment, and the ability of desert algae to survive, multiply and apply can be enhanced by artificially regulating the growth substrate of desert algae. Improve the efficiency of desert algae management of the ecological environment. At present, vegetation blankets and biological blankets are also used in ecological construction, but these biological blankets are mainly artificial biological crusts made of herbs and algae. There is no patent document for algal blankets.

发明内容SUMMARY OF THE INVENTION

本发明的目的是针对现有应用藻类治理生态环境技术的不足,提供一种藻毯及其制备方法,应用藻毯全覆盖或行列式铺设等方式治理流沙地、劣地和工程裸地的生物方法,适用于快速、长效治理裸地。The purpose of the present invention is to provide a kind of algal blanket and its preparation method in view of the deficiencies of the existing application of algae treatment ecological environment technology, and the application of algae blanket full coverage or determinant laying and other methods to control the biology of quicksand, bad land and engineering bare land The method is suitable for rapid and long-term management of bare land.

技术方案:一种藻毯,从上到下依次包括上层保护纤维织物层、藻种层、基质层、定型支撑网和底层粘结带,Technical scheme: an algal blanket, from top to bottom, comprises an upper protective fiber fabric layer, an algal seed layer, a matrix layer, a shaping support net and a bottom adhesive tape,

所述上层保护纤维织物层、所述定型支撑网、所述底层粘结带采用缝编线缝接或用改性淀粉胶粘接,The upper protective fiber fabric layer, the shaping support net, and the bottom layer adhesive tape are sewed with stitch-bonding thread or bonded with modified starch glue,

所述基质层与所述定型支撑网粘接,The matrix layer is bonded with the shaping support mesh,

所述藻种层与所述基质层粘接,The algae seed layer is bonded to the matrix layer,

所述底层粘结带为纤维织物,其两侧分别比定型支撑网的两侧宽2cm,作为藻毯相互粘接的粘接带。The bottom layer adhesive tape is a fiber fabric, the two sides of which are respectively 2 cm wider than the two sides of the shaping support net, and are used as the adhesive tape for mutual bonding of the algal blankets.

进一步地,所述上层保护纤维织物层的厚度为0.2~0.3mm。Further, the thickness of the upper protective fiber fabric layer is 0.2-0.3 mm.

进一步地,所述藻种层的厚度为0.2~0.3mm。Further, the thickness of the algae seed layer is 0.2-0.3 mm.

进一步地,所述基质层的厚度为2.4~2.6mm。Further, the thickness of the matrix layer is 2.4-2.6 mm.

进一步地,所述定型支撑网厚度为2.0~2.5mm。Further, the thickness of the shaping support net is 2.0-2.5 mm.

进一步地,所述底层粘结带的厚度为0.2~0.3mm。Further, the thickness of the bottom layer adhesive tape is 0.2-0.3 mm.

一种上述藻毯的制备方法,包括以下步骤:A preparation method of the above-mentioned algal blanket, comprising the following steps:

(1)制备营养液(1) Preparation of nutrient solution

(11)配备营养物:(11) Equipped with nutrients:

称取0.8~2.5质量份硝酸钠、0.02~0.05质量份磷酸氢二钾、0.06~0.09质量份硫酸镁、0.02~0.05质量份氯化钙、0.004~0.008质量份柠檬酸、0.004~0.008质量份柠檬铁铵、0.001~0.003质量份乙二胺四乙酸二钠盐、0.01~0.04质量份碳酸钠,将上述原料混合均匀得到混合粉体,备用;Weigh out 0.8-2.5 parts by mass of sodium nitrate, 0.02-0.05 parts by mass of dipotassium hydrogen phosphate, 0.06-0.09 parts by mass of magnesium sulfate, 0.02-0.05 parts by mass of calcium chloride, 0.004-0.008 parts by mass of citric acid, and 0.004-0.008 parts by mass Ferric ammonium citrate, 0.001-0.003 mass part of ethylenediaminetetraacetic acid disodium salt, 0.01-0.04 mass part of sodium carbonate, the above raw materials are uniformly mixed to obtain a mixed powder, which is for later use;

(12)配备微量元素溶液(12) Equipped with trace element solution

将2.86质量份H3BO3、1.81质量份MnCl2·H2O、0.222质量份ZnSO4·7H2O、0.079质量份CuSO4·5H2O、0.390质量份Na2MoO4·2H2O、0.049质量份Co(NO3)2·6H2O加入适量水中定容成1000质量份的微量元素溶液,2.86 parts by mass of H 3 BO 3 , 1.81 parts by mass of MnCl 2 ·H 2 O, 0.222 parts by mass of ZnSO 4 ·7H 2 O, 0.079 parts by mass of CuSO 4 ·5H 2 O, 0.390 parts by mass of Na 2 MoO 4 ·2H 2 O , 0.049 parts by mass of Co(NO 3 ) 2 ·6H 2 O was added to an appropriate amount of water to make a solution of trace elements of 1000 parts by mass,

(13)将步骤(11)得到的混合粉体与步骤(12)得到的微量元素溶液按质量比(1.4~1.8):1配备,混和均匀,配制成营养液;(13) prepare the mixed powder obtained in step (11) and the trace element solution obtained in step (12) in a mass ratio (1.4-1.8): 1, mix them evenly, and prepare a nutrient solution;

(2)制备基质(2) Preparation of substrate

(21)将2.0质量份改性纤维素、0.1质量份改性淀粉、20.0质量份棉毛纤维、1.0质量份步骤(1)得到的营养液、10.0质量份凹凸棒粉和66.9质量份水混合均匀得到混合物;(21) Mix 2.0 parts by mass of modified cellulose, 0.1 part by mass of modified starch, 20.0 parts by mass of cotton wool fiber, 1.0 part by mass of the nutrient solution obtained in step (1), 10.0 parts by mass of attapulgite powder and 66.9 parts by mass of water get a mixture;

(22)将步骤(21)得到的混合物搅拌均匀,制成胶体材料,即得到基质;(22) the mixture obtained in step (21) is evenly stirred to make a colloidal material, that is, a matrix is obtained;

(3)制备荒漠藻(3) Preparation of desert algae

(31)藻种获取(31) Obtaining algae species

(311)将干旱区带有荒漠藻的土壤结皮研磨,无菌水浸泡后培养,将培养的藻种用解剖针挑出,在无菌载玻片上灭菌,接入琼脂培养皿,无菌培养5~10天,得到分离和纯化后藻种;(311) grinding the soil crusts with desert algae in arid areas, immersing them in sterile water and then culturing them, picking out the cultured algal species with a dissecting needle, sterilizing them on a sterile glass slide, and inserting them into an agar petri dish, without The bacteria are cultured for 5 to 10 days to obtain algal species after separation and purification;

(312)将步骤(311)得到的分离和纯化的藻类接入装有步骤(1)得到的培养液的培养瓶中,然后在温度22~35℃,光强3500Lx,通气量3L/min的条件下,培养10~20天得到藻种;(312) inserting the separated and purified algae obtained in step (311) into a culture flask containing the culture solution obtained in step (1), and then at a temperature of 22 to 35° C., a light intensity of 3500 Lx, and a ventilation rate of 3 L/min. Under conditions, cultured for 10 to 20 days to obtain algae seeds;

(32)制备藻泥(32) Preparation of algal slime

(321)将步骤(31)得到的藻种接入装有步骤(1)得到的营养液的培养瓶,接种比例为每升营养液0.2~0.5克藻种,在温度22~35℃,光强3500Lx,通气量3L/min的条件下,通气培养藻群10~20天得到荒漠藻;(321) inserting the algal seeds obtained in step (31) into a culture bottle containing the nutrient solution obtained in step (1), the inoculation ratio is 0.2-0.5 grams of algal seeds per liter of nutrient solution, and at a temperature of 22-35 ° C, light Under the condition of strong 3500Lx and ventilation volume of 3L/min, the desert algae can be obtained by aerating and cultivating algae for 10 to 20 days;

(322)将步骤(321)得到的荒漠藻倒入过滤筛,过滤获得藻浆,再将藻浆经过离心机离心去水,制成藻泥;(322) pouring the desert algae obtained in step (321) into a filter screen, filtering to obtain algal slurry, and then centrifuging the algal slurry through a centrifuge to remove water to make algal slurry;

(4)制备藻毯(4) Preparation of algal blanket

(41)将植物纤维织物铺平整,使之成为底层粘结带,(41) Flatten the plant fiber fabric to make it a bottom adhesive tape,

(42)将植物纤维网平铺在步骤(41)得到的底层粘结带上,形成定型支撑网,底层粘结带两侧分别与定型支撑网的两侧宽2cm;(42) the plant fiber web is spread on the bottom layer adhesive tape obtained in step (41) to form a shaping support net, and both sides of the bottom layer adhesive tape are respectively 2cm wide with both sides of the shaping support net;

(43)将步骤(2)得到的基质摊压到定型支撑网上,形成基质层;(43) rolling the matrix obtained in step (2) onto the shaping support net to form a matrix layer;

(44)将步骤(3)得到的藻泥均匀喷或涂敷在基质层上,形成藻种层;(44) the algal mud obtained in step (3) is evenly sprayed or coated on the matrix layer to form an algal seed layer;

(45)在藻种层上侧覆盖植物纤维织物,使之成为上层保护织物层;(45) covering the plant fiber fabric on the upper side of the algae seed layer to make it an upper protective fabric layer;

(46)将上层保护纤维织物层、带有藻种和基质的定型支撑网、底层粘结带缝合或粘合到一起,形成一个整体,阴干后即得到藻毯。(46) The upper protective fiber fabric layer, the shaped support net with algae species and matrix, and the bottom layer adhesive tape are sewed or bonded together to form a whole, and the algal blanket is obtained after drying in the shade.

有益效果:本发明公开的一种藻毯及其制备方法具有以下有益效果:Beneficial effects: The algal blanket and its preparation method disclosed in the present invention have the following beneficial effects:

1、以纤维网为定型支撑网,将荒漠藻和改性植物纤维素保水材料等多种材料,通过生产流水线一次加工而成。将藻毯铺于地面,可形成微生物结皮,覆盖地表、防治流沙、保持水土、储存地表水分,实现流动地表的固化,治理恶劣环境的裸地,可减轻水和风的侵蚀;1. The fiber net is used as the shaped support net, and various materials such as desert algae and modified plant cellulose water-retaining materials are processed through the production line at one time. Spreading the algal blanket on the ground can form microbial crusts, cover the ground, prevent quicksand, maintain soil and water, store surface moisture, realize the solidification of the flowing surface, treat bare ground in harsh environments, and reduce water and wind erosion;

2、藻毯可应用于干旱区的流动沙地、山坡、崖、工程裸地等治理,其结构简单,操作方便,设置快速,能够在短时间内大面积的布设藻毯形成连片的固定地表,起到良好的防风固沙、保持水土及减灾作用。2. The algal blanket can be applied to the treatment of moving sand, hillsides, cliffs, engineering bare land, etc. in arid areas. It plays a good role in windbreak and sand fixation, soil and water conservation and disaster reduction.

附图说明Description of drawings

图1为本发明公开的一种藻毯的结构示意图;Fig. 1 is the structural representation of a kind of algal blanket disclosed by the present invention;

图2为本发明公开的一种藻毯的制备方法的流程图;Fig. 2 is the flow chart of the preparation method of a kind of algal blanket disclosed by the present invention;

其中:in:

1-上层保护纤维织物层1- The upper protective fiber fabric layer 2-藻种层2- Algal seed layer 3-基质层3 - Matrix layer 4-定型支撑网4-Styling support net 5-底层粘结带5- Bottom layer adhesive tape

具体实施方式:Detailed ways:

下面对本发明的具体实施方式详细说明。The specific embodiments of the present invention will be described in detail below.

具体实施例1Specific Example 1

如图1所示,一种藻毯,从上到下依次包括上层保护纤维织物层1、藻种层2、基质层3、定型支撑网4和底层粘结带5,As shown in Figure 1, a kind of algal blanket, from top to bottom, comprises an upper protective fiber fabric layer 1, an algal seed layer 2, a matrix layer 3, a shaping support net 4 and a bottom layer adhesive tape 5,

上层保护纤维织物层1、定型支撑网4、底层粘结带5采用缝编线缝接或用改性淀粉胶粘接,The upper protective fiber fabric layer 1, the shaping support net 4, and the bottom adhesive tape 5 are sewn together with stitching threads or bonded with modified starch glue.

基质层3与定型支撑网4粘接,The matrix layer 3 is bonded to the shaping support net 4,

藻种层2与基质层3粘接,The algae seed layer 2 is bonded to the matrix layer 3,

底层粘结带5为纤维织物,其两侧分别比定型支撑网4的两侧宽2cm,作为藻毯相互粘接的粘接带。The bottom layer adhesive tape 5 is a fiber fabric, the two sides of which are respectively 2 cm wider than the two sides of the shaping support net 4, as the adhesive tape for mutual bonding of the algal blankets.

进一步地,上层保护纤维织物层1的厚度为0.2mm。上层保护纤维织物层1为可降解植物纤维织物,为单层或多层植物纤维织物。保护纤维织物要求寿命大于30天,保护藻种及其基质,利于藻种生长。Further, the thickness of the upper protective fiber fabric layer 1 is 0.2 mm. The upper protective fiber fabric layer 1 is a degradable plant fiber fabric, which is a single-layer or multi-layer plant fiber fabric. The protection fiber fabric requires a life span of more than 30 days, protects the algae and its substrate, and is conducive to the growth of the algae.

进一步地,藻种层2的厚度为0.2mm。藻种为适应工程区气候条件的多种藻组合。在我国干旱荒漠区则主要为具鞘微鞘藻、爪哇伪枝藻、单岐藻、念珠藻、席藻和原球藻。荒漠藻的建群藻种为具鞘微鞘藻和爪哇伪枝藻,其比例占藻种组成的60%-90%。Further, the thickness of the algae seed layer 2 is 0.2 mm. Algae species are a variety of algae combinations adapted to the climatic conditions of the project area. In the arid desert areas of my country, the main species are Microcystheca sp., Pseudocladium javanica, Monostheca, Nostoc, S. spp. and Protococcus. The constructive algal species of desert algae are Microsphingomyces sp. and Pseudomonas javanica, which account for 60%-90% of the algal species.

进一步地,基质层3的厚度为2.4mm。为改性纤维素高分子吸水材料,起吸水保水作用,给藻提供水分、营养和着生床,要求保水能力衰减慢、易降解、不板结、对环境无害。Further, the thickness of the base layer 3 is 2.4 mm. It is a modified cellulose polymer water-absorbing material, which plays the role of water absorption and water retention, and provides water, nutrients and implantation beds for algae.

进一步地,定型支撑网4厚度为2.0mm。定型支撑网4是植物纤维网。Further, the thickness of the shaping support net 4 is 2.0 mm. The shaping support net 4 is a vegetable fiber net.

进一步地,底层粘结带5的厚度为0.2mm。底层粘结带5为可降解植物纤维织物,为单层植物纤维织物。粘结带要求寿命大于30天,保护藻种及其基质,利于藻种生长。Further, the thickness of the bottom layer adhesive tape 5 is 0.2 mm. The bottom layer adhesive tape 5 is a degradable plant fiber fabric, which is a single-layer plant fiber fabric. The adhesive tape requires a life span of more than 30 days to protect the algae and its substrate, which is conducive to the growth of the algae.

如图2所示,一种上述藻毯的制备方法,包括以下步骤:As shown in Figure 2, a preparation method of the above-mentioned algal blanket, comprising the following steps:

(1)制备营养液(1) Preparation of nutrient solution

(11)配备营养物:(11) Equipped with nutrients:

称取0.8质量份硝酸钠、0.02质量份磷酸氢二钾、0.06质量份硫酸镁、0.02质量份氯化钙、0.004质量份柠檬酸、0.004质量份柠檬铁铵、0.001质量份乙二胺四乙酸二钠盐、0.01质量份碳酸钠,将上述原料混合均匀得到混合粉体,备用;Weigh 0.8 part by mass of sodium nitrate, 0.02 part by mass of dipotassium hydrogen phosphate, 0.06 part by mass of magnesium sulfate, 0.02 part by mass of calcium chloride, 0.004 part by mass of citric acid, 0.004 part by mass of ferric ammonium citrate, and 0.001 part by mass of EDTA Disodium salt, 0.01 mass part of sodium carbonate, the above-mentioned raw materials are mixed uniformly to obtain mixed powder, which is for subsequent use;

(12)配备微量元素溶液(12) Equipped with trace element solution

将2.86质量份H3BO3、1.81质量份MnCl2·H2O、0.222质量份ZnSO4·7H2O、0.079质量份CuSO4·5H2O、0.390质量份Na2MoO4·2H2O、0.049质量份Co(NO3)2·6H2O加入适量水中定容成1000质量份的微量元素溶液,2.86 parts by mass of H 3 BO 3 , 1.81 parts by mass of MnCl 2 ·H 2 O, 0.222 parts by mass of ZnSO 4 ·7H 2 O, 0.079 parts by mass of CuSO 4 ·5H 2 O, 0.390 parts by mass of Na 2 MoO 4 ·2H 2 O , 0.049 parts by mass of Co(NO 3 ) 2 ·6H 2 O was added to an appropriate amount of water to make a solution of trace elements of 1000 parts by mass,

(13)将步骤(11)得到的混合粉体与步骤(12)得到的微量元素溶液按质量比1.4:1配备,混和均匀,配制成营养液;(13) prepare the mixed powder obtained in step (11) and the trace element solution obtained in step (12) in a mass ratio of 1.4:1, mix them evenly, and prepare a nutrient solution;

(2)制备基质(2) Preparation of substrate

(21)将2.0质量份改性纤维素、0.1质量份改性淀粉、20.0质量份棉毛纤维、1.0质量份步骤(1)得到的营养液、10.0质量份凹凸棒粉和66.9质量份水混合均匀得到混合物;(21) Mix 2.0 parts by mass of modified cellulose, 0.1 part by mass of modified starch, 20.0 parts by mass of cotton wool fiber, 1.0 part by mass of the nutrient solution obtained in step (1), 10.0 parts by mass of attapulgite powder and 66.9 parts by mass of water get a mixture;

(22)将步骤(21)得到的混合物搅拌均匀,制成胶体材料,即得到基质;(22) the mixture obtained in step (21) is evenly stirred to make a colloidal material, that is, a matrix is obtained;

(3)制备荒漠藻(3) Preparation of desert algae

(31)藻种获取(31) Obtaining algae species

(311)将干旱区带有荒漠藻的土壤结皮研磨,无菌水浸泡后培养,将培养的藻种用解剖针将藻种挑出,在无菌载玻片上灭菌,接入琼脂培养皿,无菌培养5天,得到分离和纯化后藻种;(311) grinding the soil crust with desert algae in the arid area, immersing it in sterile water, and then culturing, picking out the cultured algal species with a dissecting needle, sterilizing them on a sterile glass slide, and inserting them into agar for culture Dish, aseptically cultured for 5 days to obtain algal species after separation and purification;

(312)将步骤(311)得到的分离和纯化的藻类接入装有步骤(1)得到的培养液的培养瓶中,然后在温度22℃,光强3500Lx,通气量3L/min的条件下,培养10天得到藻种;(312) inserting the separated and purified algae obtained in step (311) into a culture flask containing the culture solution obtained in step (1), then at a temperature of 22°C, light intensity of 3500Lx, and ventilation rate of 3L/min under the conditions , cultivated for 10 days to obtain algal seeds;

(32)制备藻泥(32) Preparation of algal slime

(321)将步骤(31)得到的藻种接入装有步骤(1)得到的营养液的培养瓶,接种比例为每升营养液0.2克藻种,在温度22℃,光强3500Lx,通气量3L/min的条件下,通气培养藻群10天得到荒漠藻;(321) insert the algal seed obtained in step (31) into the culture bottle containing the nutrient solution obtained in step (1), the inoculation ratio is 0.2 grams of algal seed per liter of nutrient solution, at a temperature of 22 ° C, light intensity 3500Lx, ventilation Under the condition of 3L/min, the desert algae was obtained by cultivating algae in aeration for 10 days;

(322)将步骤(321)得到的荒漠藻倒入过滤筛,过滤获得藻浆,再将藻浆经过离心机离心去水,制成藻泥;(322) pouring the desert algae obtained in step (321) into a filter screen, filtering to obtain algal slurry, and then centrifuging the algal slurry through a centrifuge to remove water to make algal slurry;

(4)制备藻毯(4) Preparation of algal blanket

(41)将植物纤维织物铺平整,使之成为底层粘结带,(41) Flatten the plant fiber fabric to make it a bottom adhesive tape,

(42)将植物纤维网平铺在步骤(41)得到的底层粘结带上,形成定型支撑网,底层粘结带两侧分别与定型支撑网的两侧宽2cm;(42) the plant fiber web is spread on the bottom layer adhesive tape obtained in step (41) to form a shaping support net, and both sides of the bottom layer adhesive tape are respectively 2cm wide with both sides of the shaping support net;

(43)将步骤(2)得到的基质摊压到定型支撑网上,形成基质层;(43) rolling the matrix obtained in step (2) onto the shaping support net to form a matrix layer;

(44)将步骤(3)得到的藻泥均匀喷或涂敷在基质层上,形成藻种层;(44) the algal mud obtained in step (3) is evenly sprayed or coated on the matrix layer to form an algal seed layer;

(45)在藻种层上侧覆盖植物纤维织物,使之成为上层保护织物层;(45) covering the plant fiber fabric on the upper side of the algae seed layer to make it an upper protective fabric layer;

(46)将上层保护纤维织物层、带有藻种和基质的定型支撑网、底层粘结带缝合到一起,形成一个整体,阴干后即得到藻毯。(46) The upper protective fiber fabric layer, the shaped support net with algae species and matrix, and the bottom layer adhesive tape are sewed together to form a whole, and the algal blanket is obtained after drying in the shade.

具体实施例2Specific embodiment 2

一种藻毯,从上到下依次包括上层保护纤维织物层1、藻种层2、基质层3、定型支撑网4和底层粘结带5,An algal blanket, from top to bottom, comprises an upper protective fiber fabric layer 1, an algae seed layer 2, a matrix layer 3, a shaping support net 4 and a bottom layer adhesive tape 5,

上层保护纤维织物层1、定型支撑网4、底层粘结带5采用缝编线缝接或用改性淀粉胶粘接,The upper protective fiber fabric layer 1, the shaping support net 4, and the bottom adhesive tape 5 are sewn together with stitching threads or bonded with modified starch glue.

基质层3与定型支撑网4粘接,The matrix layer 3 is bonded to the shaping support net 4,

藻种层2与基质层3粘接,The algae seed layer 2 is bonded to the matrix layer 3,

底层粘结带5为纤维织物,其两侧分别比定型支撑网4的两侧宽2cm,作为藻毯相互粘接的粘接带。The bottom layer adhesive tape 5 is a fiber fabric, the two sides of which are respectively 2 cm wider than the two sides of the shaping support net 4, as the adhesive tape for mutual bonding of the algal blankets.

进一步地,上层保护纤维织物层1的厚度为0.3mm。上层保护纤维织物层1为可降解植物纤维织物,为单层或多层植物纤维织物。保护纤维织物要求寿命大于30天,保护藻种及其基质,利于藻种生长。Further, the thickness of the upper protective fiber fabric layer 1 is 0.3 mm. The upper protective fiber fabric layer 1 is a degradable plant fiber fabric, which is a single-layer or multi-layer plant fiber fabric. The protection fiber fabric requires a life span of more than 30 days, protects the algae and its substrate, and is conducive to the growth of the algae.

进一步地,藻种层2的厚度为0.3mm。藻种为适应工程区气候条件的多种藻组合。在我国干旱荒漠区则主要为具鞘微鞘藻、爪哇伪枝藻、单岐藻、念珠藻、席藻和原球藻。荒漠藻的建群藻种为具鞘微鞘藻和爪哇伪枝藻,其比例占藻种组成的60%~90%。Further, the thickness of the algae seed layer 2 is 0.3 mm. Algae species are a variety of algae combinations adapted to the climatic conditions of the project area. In the arid desert areas of my country, the main species are Microcythenia encasei, Pseudocladium javanica, Monospermia, Nostoc, S. spp and Protococcus. The algal species of desert algae are Microsphingomyces sp. and Pseudomonas javanica, which account for 60% to 90% of the algal species.

进一步地,基质层3的厚度为2.6mm。为改性纤维素高分子吸水材料,起吸水保水作用,给藻提供水分、营养和着生床,要求保水能力衰减慢、易降解、不板结、对环境无害。Further, the thickness of the base layer 3 is 2.6 mm. It is a modified cellulose polymer water-absorbing material, which plays the role of water absorption and water retention, and provides water, nutrients and implantation beds for algae.

进一步地,定型支撑网4厚度为2.5mm。定型支撑网4是植物纤维网。Further, the thickness of the shaping support net 4 is 2.5mm. The shaping support net 4 is a vegetable fiber net.

进一步地,底层粘结带5的厚度为0.3mm。底层粘结带5为可降解植物纤维织物,为单层植物纤维织物。粘结带要求寿命大于30天,保护藻种及其基质,利于藻种生长。Further, the thickness of the bottom layer adhesive tape 5 is 0.3 mm. The bottom layer adhesive tape 5 is a degradable plant fiber fabric, which is a single-layer plant fiber fabric. The adhesive tape requires a life span of more than 30 days to protect the algae and its substrate, which is conducive to the growth of the algae.

一种上述藻毯的制备方法,包括以下步骤:A preparation method of the above-mentioned algal blanket, comprising the following steps:

(1)制备营养液(1) Preparation of nutrient solution

(11)配备营养物:(11) Equipped with nutrients:

称取2.5质量份硝酸钠、0.05质量份磷酸氢二钾、0.09质量份硫酸镁、0.05质量份氯化钙、0.008质量份柠檬酸、0.008质量份柠檬铁铵、0.003质量份乙二胺四乙酸二钠盐、0.04质量份碳酸钠,将上述原料混合均匀得到混合粉体,备用;Weigh 2.5 parts by mass of sodium nitrate, 0.05 part by mass of dipotassium hydrogen phosphate, 0.09 part by mass of magnesium sulfate, 0.05 part by mass of calcium chloride, 0.008 part by mass of citric acid, 0.008 part by mass of ferric ammonium citrate, and 0.003 part by mass of EDTA Disodium salt and 0.04 parts by mass of sodium carbonate, the above-mentioned raw materials are mixed uniformly to obtain a mixed powder, which is for subsequent use;

(12)配备微量元素溶液(12) Equipped with trace element solution

将2.86质量份H3BO3、1.81质量份MnCl2·H2O、0.222质量份ZnSO4·7H2O、0.079质量份CuSO4·5H2O、0.390质量份Na2MoO4·2H2O、0.049质量份Co(NO3)2·6H2O加入适量水中定容成1000质量份的微量元素溶液,2.86 parts by mass of H 3 BO 3 , 1.81 parts by mass of MnCl 2 ·H 2 O, 0.222 parts by mass of ZnSO 4 ·7H 2 O, 0.079 parts by mass of CuSO 4 ·5H 2 O, 0.390 parts by mass of Na 2 MoO 4 ·2H 2 O , 0.049 parts by mass of Co(NO 3 ) 2 ·6H 2 O was added to an appropriate amount of water to make a solution of trace elements of 1000 parts by mass,

(13)将步骤(11)得到的混合粉体与步骤(12)得到的微量元素溶液按质量比1.8:1配备,混和均匀,配制成营养液;(13) prepare the mixed powder obtained in step (11) and the trace element solution obtained in step (12) in a mass ratio of 1.8:1, mix them evenly, and prepare a nutrient solution;

(2)制备基质(2) Preparation of substrate

(21)将2.0质量份改性纤维素、0.1质量份改性淀粉、20.0质量份棉毛纤维、1.0质量份步骤(1)得到的营养液、10.0质量份凹凸棒粉和66.9质量份水混合均匀得到混合物;(21) Mix 2.0 parts by mass of modified cellulose, 0.1 part by mass of modified starch, 20.0 parts by mass of cotton wool fiber, 1.0 part by mass of the nutrient solution obtained in step (1), 10.0 parts by mass of attapulgite powder and 66.9 parts by mass of water get a mixture;

(22)将步骤(21)得到的混合物搅拌均匀,制成胶体材料,即得到基质;(22) the mixture obtained in step (21) is evenly stirred to make a colloidal material, that is, a matrix is obtained;

(3)制备荒漠藻(3) Preparation of desert algae

(31)藻种获取(31) Obtaining algae species

(311)将干旱区带有荒漠藻的土壤结皮研磨,无菌水浸泡后培养,将培养的藻种用解剖针挑出,在无菌载玻片上灭菌,接入琼脂培养皿,无菌培养10天,得到分离和纯化后藻种;(311) grinding the soil crusts with desert algae in arid areas, immersing them in sterile water and then culturing them, picking out the cultured algal species with a dissecting needle, sterilizing them on a sterile glass slide, and inserting them into an agar petri dish, without The bacteria were cultured for 10 days to obtain the algal species after separation and purification;

(312)将步骤(311)得到的分离和纯化的藻类接入装有步骤(1)得到的培养液的培养瓶中,然后在温度35℃,光强3500Lx,通气量3L/min的条件下,培养20天得到藻种;(312) inserting the separated and purified algae obtained in step (311) into a culture flask containing the culture solution obtained in step (1), then at a temperature of 35°C, light intensity of 3500Lx, and ventilation rate of 3L/min under the conditions , cultivated for 20 days to obtain algal seeds;

(32)制备藻泥(32) Preparation of algal slime

(321)将步骤(31)得到的藻种接入装有步骤(1)得到的营养液的培养瓶,接种比例为每升营养液0.5克藻种,在温度35℃,光强3500Lx,通气量3L/min的条件下,通气培养藻群20天得到荒漠藻;(321) insert the algae seed obtained in step (31) into the culture flask that is equipped with the nutrient solution obtained in step (1), the inoculation ratio is 0.5 grams of algae seed per liter of nutrient solution, at a temperature of 35°C, light intensity 3500Lx, ventilation Under the condition of 3L/min, the desert algae was obtained by cultivating the algae in aeration for 20 days;

(322)将步骤(321)得到的荒漠藻倒入过滤筛,过滤获得藻浆,再将藻浆经过离心机离心去水,制成藻泥;(322) pouring the desert algae obtained in step (321) into a filter screen, filtering to obtain algal slurry, and then centrifuging the algal slurry through a centrifuge to remove water to make algal slurry;

(4)制备藻毯(4) Preparation of algal blanket

(41)将植物纤维织物铺平整,使之成为底层粘结带,(41) Flatten the plant fiber fabric to make it a bottom adhesive tape,

(42)将植物纤维网平铺在步骤(41)得到的底层粘结带上,形成定型支撑网,底层粘结带两侧分别与定型支撑网的两侧宽2cm;(42) the plant fiber web is spread on the bottom layer adhesive tape obtained in step (41) to form a shaping support net, and both sides of the bottom layer adhesive tape are respectively 2cm wide with both sides of the shaping support net;

(43)将步骤(2)得到的基质摊压到定型支撑网上,形成基质层;(43) rolling the matrix obtained in step (2) onto the shaping support net to form a matrix layer;

(44)将步骤(3)得到的藻泥均匀喷或涂敷在基质层上,形成藻种层;(44) the algal mud obtained in step (3) is evenly sprayed or coated on the matrix layer to form an algal seed layer;

(45)在藻种层上侧覆盖植物纤维织物,使之成为上层保护织物层;(45) covering the plant fiber fabric on the upper side of the algae seed layer to make it an upper protective fabric layer;

(46)将上层保护纤维织物层、带有藻种和基质的定型支撑网、底层粘结带粘合到一起,形成一个整体,阴干后即得到藻毯。(46) Bonding the upper protective fiber fabric layer, the shaped support net with algae species and matrix, and the bottom layer adhesive tape together to form a whole, and the algal blanket is obtained after drying in the shade.

具体实施例3Specific embodiment 3

一种藻毯,从上到下依次包括上层保护纤维织物层1、藻种层2、基质层3、定型支撑网4和底层粘结带5,An algal blanket, from top to bottom, comprises an upper protective fiber fabric layer 1, an algae seed layer 2, a matrix layer 3, a shaping support net 4 and a bottom layer adhesive tape 5,

上层保护纤维织物层1、定型支撑网4、底层粘结带5采用缝编线缝接或用改性淀粉胶粘接,The upper protective fiber fabric layer 1, the shaping support net 4, and the bottom adhesive tape 5 are sewn together with stitching threads or bonded with modified starch glue.

基质层3与定型支撑网4粘接,The matrix layer 3 is bonded to the shaping support net 4,

藻种层2与基质层3粘接,The algae seed layer 2 is bonded to the matrix layer 3,

底层粘结带5为纤维织物,其两侧分别比定型支撑网4的两侧宽2cm,作为藻毯相互粘接的粘接带。The bottom layer adhesive tape 5 is a fiber fabric, the two sides of which are respectively 2 cm wider than the two sides of the shaping support net 4, as the adhesive tape for mutual bonding of the algal blankets.

进一步地,上层保护纤维织物层1的厚度为0.25mm。上层保护纤维织物层1为可降解植物纤维织物,为单层或多层植物纤维织物。保护纤维织物要求寿命大于30天,保护藻种及其基质,利于藻种生长。Further, the thickness of the upper protective fiber fabric layer 1 is 0.25 mm. The upper protective fiber fabric layer 1 is a degradable plant fiber fabric, which is a single-layer or multi-layer plant fiber fabric. The protection fiber fabric requires a life span of more than 30 days, protects the algae and its substrate, and is conducive to the growth of the algae.

进一步地,藻种层2的厚度为0.25mm。藻种为适应工程区气候条件的多种藻组合。在我国干旱荒漠区则主要为具鞘微鞘藻、爪哇伪枝藻、单岐藻、念珠藻、席藻和原球藻。荒漠藻的建群藻种为具鞘微鞘藻和爪哇伪枝藻,其比例占藻种组成的60%-90%。Further, the thickness of the algae seed layer 2 is 0.25mm. Algae species are a variety of algae combinations adapted to the climatic conditions of the project area. In the arid desert areas of my country, the main species are Microcystheca sp., Pseudocladium javanica, Monostheca, Nostoc, S. spp. and Protococcus. The building algal species of desert algae are Microsphingomyces sp. and Pseudomonas javanica, which account for 60%-90% of the algal species.

进一步地,基质层3的厚度为2.5mm。为改性纤维素高分子吸水材料,起吸水保水作用,给藻提供水分、营养和着生床,要求保水能力衰减慢、易降解、不板结、对环境无害。Further, the thickness of the base layer 3 is 2.5 mm. It is a modified cellulose polymer water-absorbing material, which plays the role of water absorption and water retention, and provides water, nutrients and implantation beds for algae.

进一步地,定型支撑网厚度为2.3mm。定型支撑网是植物纤维网。Further, the thickness of the shaping support mesh is 2.3mm. The shaped support net is a vegetable fiber net.

进一步地,底层粘结带5的厚度为0.25mm。底层粘结带5为可降解植物纤维织物,为单层植物纤维织物。粘结带要求寿命大于30天,保护藻种及其基质,利于藻种生长。Further, the thickness of the bottom layer adhesive tape 5 is 0.25 mm. The bottom layer adhesive tape 5 is a degradable plant fiber fabric, which is a single-layer plant fiber fabric. The adhesive tape requires a life span of more than 30 days to protect the algae and its substrate, which is conducive to the growth of the algae.

一种上述藻毯的制备方法,包括以下步骤:A preparation method of the above-mentioned algal blanket, comprising the following steps:

(1)制备营养液(1) Preparation of nutrient solution

(11)配备营养物:(11) Equipped with nutrients:

称取1质量份硝酸钠、0.04质量份磷酸氢二钾、0.07质量份硫酸镁、0.04质量份氯化钙、0.006质量份柠檬酸、0.006质量份柠檬铁铵、0.002质量份乙二胺四乙酸二钠盐、0.03质量份碳酸钠,将上述原料混合均匀得到混合粉体,备用;Weigh 1 part by mass of sodium nitrate, 0.04 part by mass of dipotassium hydrogen phosphate, 0.07 part by mass of magnesium sulfate, 0.04 part by mass of calcium chloride, 0.006 part by mass of citric acid, 0.006 part by mass of ferric ammonium citrate, and 0.002 part by mass of EDTA Disodium salt, 0.03 parts by mass of sodium carbonate, the above-mentioned raw materials are mixed uniformly to obtain a mixed powder, which is for subsequent use;

(12)配备微量元素溶液(12) Equipped with trace element solution

将2.86质量份H3BO3、1.81质量份MnCl2·H2O、0.222质量份ZnSO4·7H2O、0.079质量份CuSO4·5H2O、0.390质量份Na2MoO4·2H2O、0.049质量份Co(NO3)2·6H2O加入适量水中定容成1000质量份的微量元素溶液,2.86 parts by mass of H 3 BO 3 , 1.81 parts by mass of MnCl 2 ·H 2 O, 0.222 parts by mass of ZnSO 4 ·7H 2 O, 0.079 parts by mass of CuSO 4 ·5H 2 O, 0.390 parts by mass of Na 2 MoO 4 ·2H 2 O , 0.049 parts by mass of Co(NO 3 ) 2 ·6H 2 O was added to an appropriate amount of water to make a solution of trace elements of 1000 parts by mass,

(13)将步骤(11)得到的混合粉体与步骤(12)得到的微量元素溶液按质量比1.6:1配备,混和均匀,配制成营养液;(13) prepare the mixed powder obtained in step (11) and the trace element solution obtained in step (12) in a mass ratio of 1.6:1, mix them evenly, and prepare a nutrient solution;

(2)制备基质(2) Preparation of substrate

(21)将2.0质量份改性纤维素、0.1质量份改性淀粉、20.0质量份棉毛纤维、1.0质量份步骤(1)得到的营养液、10.0质量份凹凸棒粉和66.9质量份水混合均匀得到混合物;(21) Mix 2.0 parts by mass of modified cellulose, 0.1 part by mass of modified starch, 20.0 parts by mass of cotton wool fiber, 1.0 part by mass of the nutrient solution obtained in step (1), 10.0 parts by mass of attapulgite powder and 66.9 parts by mass of water get a mixture;

(22)将步骤(21)得到的混合物搅拌均匀,制成胶体材料,即得到基质;(22) the mixture obtained in step (21) is evenly stirred to make a colloidal material, that is, a matrix is obtained;

(3)制备荒漠藻(3) Preparation of desert algae

(31)藻种获取(31) Obtaining algae species

(311)将干旱区带有荒漠藻的土壤结皮研磨,无菌水浸泡后培养,将培养的藻种用解剖针将藻种挑出,在无菌载玻片上灭菌,接入琼脂培养皿,无菌培养8天,得到分离和纯化后藻种;(311) grinding soil crusts with desert algae in arid areas, immersing them in sterile water and then culturing them, picking out the cultured algal species with a dissecting needle, sterilizing them on a sterile glass slide, and inserting them into agar for culture Dish, aseptically cultured for 8 days to obtain algal species after separation and purification;

(312)将步骤(311)得到的分离和纯化的藻类接入装有步骤(1)得到的培养液的培养瓶中,然后在温度30℃,光强3500Lx,通气量3L/min的条件下,培养15天得到藻种;(312) inserting the separated and purified algae obtained in step (311) into a culture flask containing the culture solution obtained in step (1), and then at a temperature of 30° C., light intensity of 3500Lx, and ventilation under the conditions of 3L/min , cultivated for 15 days to obtain algae seeds;

(32)制备藻泥(32) Preparation of algal slime

(321)将步骤(31)得到的藻种接入装有步骤(1)得到的营养液的培养瓶,接种比例为每升营养液0.3克藻种,在温度30℃,光强3500Lx,通气量3L/min的条件下,通气培养藻群15天得到荒漠藻;(321) insert the algal seed obtained in step (31) into the culture bottle that is equipped with the nutrient solution obtained in step (1), the inoculation ratio is 0.3 grams of algal seed per liter of nutrient solution, at a temperature of 30 ° C, light intensity 3500Lx, ventilation Under the condition of 3L/min, the desert algae was obtained by cultivating algae in aeration for 15 days;

(322)将步骤(321)得到的荒漠藻倒入过滤筛,过滤获得藻浆,再将藻浆经过离心机离心去水,制成藻泥;(322) pouring the desert algae obtained in step (321) into a filter screen, filtering to obtain algal slurry, and then centrifuging the algal slurry through a centrifuge to remove water to make algal slurry;

(4)制备藻毯(4) Preparation of algal blanket

(41)将植物纤维织物铺平整,使之成为底层粘结带,(41) Flatten the plant fiber fabric to make it a bottom adhesive tape,

(42)将植物纤维网平铺在步骤(41)得到的底层粘结带上,形成定型支撑网,底层粘结带两侧分别与定型支撑网的两侧宽2cm;(42) the plant fiber web is spread on the bottom layer adhesive tape obtained in step (41) to form a shaping support net, and both sides of the bottom layer adhesive tape are respectively 2cm wide with both sides of the shaping support net;

(43)将步骤(2)得到的基质摊压到定型支撑网上,形成基质层;(43) rolling the matrix obtained in step (2) onto the shaping support net to form a matrix layer;

(44)将步骤(3)得到的藻泥均匀喷或涂敷在基质层上,形成藻种层;(44) the algal mud obtained in step (3) is evenly sprayed or coated on the matrix layer to form an algal seed layer;

(45)在藻种层上侧覆盖植物纤维织物,使之成为上层保护织物层;(45) covering the plant fiber fabric on the upper side of the algae seed layer to make it an upper protective fabric layer;

(46)将上层保护纤维织物层、带有藻种和基质的定型支撑网、底层粘结带粘合到一起,形成一个整体,阴干后即得到藻毯。(46) Bonding the upper protective fiber fabric layer, the shaped support net with algae species and matrix, and the bottom layer adhesive tape together to form a whole, and the algal blanket is obtained after drying in the shade.

上面对本发明的实施方式做了详细说明。但是本发明并不限于上述实施方式,在所属技术领域普通技术人员所具备的知识范围内,还可以在不脱离本发明宗旨的前提下做出各种变化。The embodiments of the present invention have been described in detail above. However, the present invention is not limited to the above-mentioned embodiments, and various changes can be made within the scope of knowledge possessed by those skilled in the art without departing from the spirit of the present invention.

Claims (1)

1. An algae blanket is characterized by sequentially comprising an upper layer protective fiber fabric layer, an algae seed layer, a matrix layer, a shaping support net and a bottom layer adhesive tape from top to bottom,
the upper layer protective fiber fabric layer, the shaping support net and the bottom layer bonding belt are sewn by adopting sewing threads or bonded by adopting modified starch glue,
the substrate layer is bonded with the shaping support net,
the algae seed layer is adhered to the matrix layer,
the bottom layer bonding tape is a fiber fabric, and two sides of the bottom layer bonding tape are respectively wider than two sides of the shaping support net by 2cm and are used as bonding tapes for bonding the algal blankets; the thickness of the upper protective fiber fabric layer is 0.2-0.3 mm; the thickness of the algae seed layer is 0.2-0.3 mm; the thickness of the substrate layer is 2.4-2.6 mm; the thickness of the shaping support net is 2.0-2.5 mm; the thickness of the bottom layer adhesive tape is 0.2-0.3 mm;
the preparation method of the algal blanket comprises the following steps:
(1) preparation of nutrient solution
(11) Preparing nutrients:
weighing 0.8-2.5 parts by mass of sodium nitrate, 0.02-0.05 part by mass of dipotassium hydrogen phosphate, 0.06-0.09 part by mass of magnesium sulfate, 0.02-0.05 part by mass of calcium chloride, 0.004-0.008 part by mass of citric acid, 0.004-0.008 part by mass of ferric ammonium citrate, 0.001-0.003 part by mass of disodium ethylenediamine tetraacetic acid and 0.01-0.04 part by mass of sodium carbonate, and uniformly mixing the raw materials to obtain mixed powder for later use;
(12) preparing a solution of trace elements
2.86 parts by mass of H3BO31.81 parts by mass of MnCl2·H2O, 0.222 parts by mass of ZnSO4·7H2O, 0.079 parts by mass of CuSO4·5H2O, 0.390 part by mass of Na2MoO4·2H2O, 0.049 part by mass of Co (NO)3)2·6H2Adding O into a proper amount of water to prepare 1000 parts by mass of trace element solution,
(13) mixing the mixed powder obtained in the step (11) with the trace element solution obtained in the step (12) according to the mass ratio of (1.4-1.8): 1, preparing and uniformly mixing to prepare nutrient solution;
(2) preparation of the substrate
(21) Uniformly mixing 2.0 parts by mass of modified cellulose, 0.1 part by mass of modified starch, 20.0 parts by mass of cotton wool fibers, 1.0 part by mass of the nutrient solution obtained in the step (1), 10.0 parts by mass of attapulgite powder and 66.9 parts by mass of water to obtain a mixture;
(22) uniformly stirring the mixture obtained in the step (21) to prepare a colloid material, namely obtaining a matrix;
(3) preparation of desert algae
(31) Algal species acquisition
(311) Grinding the soil crust with desert algae in arid region, soaking in sterile water, culturing, picking out cultured algae seeds with dissecting needle, sterilizing on sterile glass slide,
inoculating into an agar culture dish, and performing aseptic culture for 5-10 days to obtain separated and purified algae seeds;
(312) inoculating the separated and purified algae obtained in the step (311) into a culture bottle containing the culture solution obtained in the step (1), then, at the temperature of 22-35 ℃,
culturing for 10-20 days under the conditions that the light intensity is 3500Lx and the ventilation volume is 3L/min to obtain algae seeds;
(32) preparation of algae mud
(321) Inoculating the algae seeds obtained in the step (31) into a culture bottle filled with the nutrient solution obtained in the step (1), inoculating algae seeds in a proportion of 0.2-0.5 g per liter of nutrient solution, and performing aerobic culture on algae groups for 10-20 days at the temperature of 22-35 ℃, the light intensity of 3500Lx and the ventilation volume of 3L/min to obtain desert algae;
(322) pouring the desert algae obtained in the step (321) into a filter sieve, filtering to obtain algae slurry, and centrifuging the algae slurry through a centrifuge to remove water to prepare algae mud;
(4) preparation of algal blanket
(41) The plant fiber fabric is spread flatly to form a bottom layer binding belt,
(42) flatly paving the plant fiber net on the bottom layer bonding belt obtained in the step (41) to form a shaping support net, wherein the width of two sides of the bottom layer bonding belt is 2cm with that of two sides of the shaping support net;
(43) spreading the substrate obtained in the step (2) on a shaping support net to form a substrate layer;
(44) uniformly spraying or coating the algae mud obtained in the step (3) on the matrix layer to form an algae seed layer;
(45) covering the upper side of the algae seed layer with a plant fiber fabric to form an upper protective fabric layer;
(46) sewing or adhering the upper protective fiber fabric layer, the shaping support net with the algae seeds and the matrix and the bottom adhesive tape together to form a whole, and drying in the shade to obtain the algae blanket.
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