CN119453375A

CN119453375A - Ruminant rumen bypass coating preparation and preparation method and application thereof

Info

Publication number: CN119453375A
Application number: CN202510051650.7A
Authority: CN
Inventors: 苏华维; 张宏亮; 何阳; 张嗣禹
Original assignee: China Agricultural University
Current assignee: China Agricultural University
Priority date: 2025-01-14
Filing date: 2025-01-14
Publication date: 2025-02-18

Abstract

The present invention proposes a rumen-passing coating preparation for ruminants and a preparation method and application thereof. The rumen-passing coating preparation includes a core material and a coating, and the coating solution for preparing the coating includes, by weight: 8-10 parts of soybean lecithin oil; 2-3 parts of reducing sugar; 18-20 parts of fat; the coating solution is prepared as follows: 8-10 parts of soybean lecithin oil are heated and melted at a high temperature of 60-90°C, 2-3 parts of reducing sugar are added to a molten reaction pot and stirred until melted, and the material is kept warm at 50°C after the reaction turns brown; another 18-20 parts of fat are heated and melted, the material obtained in the previous order is mixed with it, and stirred evenly to form a mixed solution, which is the coating solution. The present invention utilizes the Maillard reaction between lecithin and reducing sugar to produce a product with excellent rumen-passing and intestinal release effects, and the obtained product has high purity, good quality, and high small intestinal release rate, improves the vitality of immune cells, and enhances disease resistance and anti-stress capabilities.

Description

Ruminant rumen bypass coating preparation and preparation method and application thereof

Technical Field

The invention relates to the technical field of animal feeding, in particular to a feed additive, and in particular relates to a ruminant rumen bypass coating preparation, a preparation method and application thereof.

Background

In the livestock industry, high quality meat, eggs and milk are increasingly concerned, so researchers have explored and developed a plurality of nutrients, such as enzyme preparations, vitamin preparations, amino acids and the like, capable of improving animal productivity. Monogastric animals can digest and absorb these substances directly, whereas ruminants often require ruminal treatment of nutrients due to the specificity of their ruminal digestion.

The most commonly used rumen bypass technology at present comprises physical treatment, chemical treatment, coating, microencapsulation and other technologies. In the process of coating nutrients, fluidized bed spray coating treatment is mostly used, and the rumen bypass material is mostly used with polypropylene resin IV as a coating material, so that the effect is better. However, release of acidic conditions in abomasum for some nutrients sensitive to environmental conditions such as enzyme preparations can inactivate enzymes, for example:

The Chinese patent application 201910501812.7 discloses a rumen bypass protection amylase and a preparation method thereof, wherein polypropylene resin IV and chitosan are used as coating materials, the feed amylase is possibly inactivated under the acid condition of abomasum, and hydrogenated vegetable oil and the like are released in the small intestine under the degradation of the pancreatic enzyme of the small intestine, but the film forming property is poor and has a certain disturbing effect on the microbial flora of the rumen. In addition, in the investigation and experiment of the materials, it was found that the hydrogenated vegetable oil was lower in release rate in the small intestine although the rumen bypass rate was higher because it was saturated fatty acid, and rumen microbial degradation was less.

Chinese patent application 202210791385.2 discloses a ruminant rumen bypass preparation, a preparation method and application thereof, wherein the ratio of polypropylene resin to absolute ethyl alcohol in the method is 1:1, the use amount of the ethyl alcohol can be greatly reduced, but a large amount of lumps can be formed by adding the ethyl alcohol melt into high-temperature molten grease, and the conditions of no dissolution can be achieved.

Chinese patent application 202011434613.8 discloses a rumen bypass product and a production method thereof, which utilizes white carbon black to adsorb palm oil to prepare the rumen bypass product, the release rate of small intestine is stable but not high, the specific gravity of the rumen bypass product is lower than that of rumen fluid between 0.9 and 1.0, and the rumen bypass product suspended in the rumen fluid is easy to cause rumen bypass behavior to be chewed by mouth again so as to damage the rumen bypass product.

Chinese patent application 201910441320.3 discloses a rumen bypass preparation for ruminant animals, and a preparation method and a device thereof, wherein high molecular materials such as cellulose ester, cellulose ether, poly (meth) acrylate, acrylic acid ester, etc. as coating dry powder materials may affect rumen fermentation, and the coating material has too small specific gravity, which may cause incomplete coating.

In summary, even if the rumen bypass rate of the product is increased, the failure to release the biological activity is a huge loss of the product, and the increase in the dosage corresponds to the increase in the production cost. Therefore, there is a need to find a way to improve the intestinal release rate of grease while increasing the rumen bypass rate.

Disclosure of Invention

In view of the shortcomings of the prior art, the invention provides a ruminant rumen bypass coating preparation, and a preparation method and application thereof, so as to solve one or more technical problems in the prior art.

In order to achieve the above purpose, the technical scheme adopted by the invention is as follows:

the invention provides a ruminant rumen bypass coating preparation, which comprises a core material and a coating, wherein a coating liquid for preparing the coating comprises the following components in parts by weight:

8-10 parts of soybean lecithin oil;

2-3 parts of reducing sugar;

18-20 parts of grease;

the coating liquid is prepared by the following method:

heating 8-10 parts of soybean phospholipid oil at 60-90 ℃ to melt, adding 2-3 parts of reducing sugar into a melted reaction kettle, stirring until the materials melt, and keeping the temperature at 50 ℃ after the materials react to be brown yellow;

And heating and melting 18-20 parts of grease, mixing the materials obtained in the previous step with the grease, and uniformly stirring the mixture to form a mixed solution, namely the coating liquid.

Preferably, the soybean phospholipid oil is a solid paste, and mainly contains lecithin, cephalin, inositol phospholipid, phosphatidylserine and phosphatidic acid.

Preferably, the reducing sugar is one or more of glucose, fructose, galactose, lactose and xylose.

Preferably, the fat is a fatty acid having 14 or more carbon atoms.

Preferably, the oil is one or more of stearic acid, glyceryl tristearate, glyceryl tripalmitate, coconut oil, hydrogenated palm oil, soybean wax and carnauba wax, hydrogenated soybean oil, eicosanoic acid, iso-oleic acid, myristic acid, lauric acid, palmitic acid.

The second aspect of the invention provides a preparation method of the rumen bypass coating preparation, which comprises the following steps:

1) Adding absolute ethyl alcohol and talcum powder into active ingredient powder in a core material, extruding the mixed material through a screen, and rounding particles;

2) Drying the rounded particles, cooling to room temperature after drying, and selecting particles with 20-30 meshes to be reserved as core materials;

3) The dry mass of the core material and the coating liquid is added according to (5-7) that (3-5);

4) And (3) performing bottom spray coating by adopting a multifunctional fluidized bed granulating and coating machine, cooling to room temperature after coating material spraying, and sieving to obtain the rumen bypass coating preparation.

Preferably, the rumen bypass coating preparation is obtained by cooling to room temperature after spraying the coating material and sieving with a 15-25 mesh sieve.

The third aspect of the invention provides a device for realizing the preparation method, which comprises a coating device, wherein the coating device is a multifunctional fluidized bed granulating and coating machine, the material yield is more than 99%, the water content of a finished product is less than 0.5%, fluidization and air flow conveying are combined through bottom spraying operation, the same-direction spray liquid and a spray nozzle are buried in the material, the probability of fluidization of particles through spray fog particles is high, and the particles are highly dense in a spray liquid area.

In a fourth aspect, the present invention provides a use of a rumen bypass coating formulation according to the above in the preparation of a feed additive for ruminants.

In a fifth aspect, the present invention provides a use of a rumen bypass coating formulation according to the present invention in the manufacture of a medicament for ruminants.

Compared with the prior art, the ruminant rumen bypass coating preparation, the preparation method and the application thereof have the beneficial effects that the soybean phospholipid oil, the reducing sugar and the grease are adopted as raw materials for preparing the coating, and the obtained product has high purity, good quality and high small intestine release rate. In particular, at least the following beneficial effects can be brought about:

(1) The rumen bypass coating preparation provided by the invention contains soybean lecithin and lecithin, a Maillard reaction product is generated by utilizing Maillard reaction between phosphatidylethanolamine and reducing sugar contained in the phospholipid, and the Maillard product and grease are mixed and sprayed, so that the degradation of fatty acid in the grease, especially hydrogenated vegetable oil, in the small intestine is promoted under the premise of ensuring high rumen bypass rate.

(2) The rumen bypass coating preparation provided by the invention contains lecithin, wherein the lecithin has the advantages of improving the digestibility of fat-free nutrients such as fat-soluble vitamins, controlling the running and absorption of cholesterol, providing energy, having antioxidant property, improving the metabolic function of organisms, improving the activity of immune cells and enhancing the disease resistance and stress resistance.

It should be understood that the implementation of any of the embodiments of the invention is not intended to simultaneously possess or achieve some or all of the above-described benefits.

Drawings

In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings used in the description of the embodiments or the prior art will be briefly described below. It will be apparent to those skilled in the art from this disclosure that the drawings described below are merely exemplary and that other embodiments may be derived from the drawings provided without undue effort.

The structures, proportions, sizes, etc. shown in this description are shown only with the understanding that those skilled in the art will understand and read the disclosure, and are not intended to limit the scope of the invention, which is defined in the claims, but rather in a manner that does not have a technical essential significance, any structural modification, proportional change or size adjustment should still fall within the scope of the disclosure without affecting the efficacy and achievement of the present invention.

Fig. 1 is a schematic diagram of a core material for preparing a rumen bypass coating preparation according to an embodiment of the present invention;

Fig. 2 is a schematic diagram of a rumen bypass coating preparation according to an embodiment of the present invention;

Fig. 3 is a schematic view of rumen bypass coating preparation product in water according to an embodiment of the present invention.

Detailed Description

For the purpose of making the objects, technical solutions and advantages of the embodiments of the present invention more apparent, the embodiments of the present invention will be described in further detail with reference to the embodiments and the accompanying drawings. The exemplary embodiments of the present invention and their descriptions herein are for the purpose of explaining the present invention, but are not to be construed as limiting the invention.

It should be understood that the terms "comprises/comprising," "consists of," or any other variation thereof, are intended to cover a non-exclusive inclusion, such that a product, apparatus, process, or method that comprises a list of elements does not include only those elements but may include other elements not expressly listed or inherent to such product, apparatus, process, or method as desired. Without further limitation, elements defined by the phrases "including/comprising" and "consisting of" do not exclude the presence of additional identical elements in a product, apparatus, process or method comprising the elements.

Raw materials in the examples of the present invention are commercially available unless otherwise specified.

The rumen bypass coating preparation for ruminants comprises a core material and a coating, wherein the coating liquid is prepared from 8-10 parts by weight of soybean phospholipid oil, 2-3 parts by weight of reducing sugar and 18-20 parts by weight of oil, the coating liquid is prepared by heating and melting 8-10 parts by weight of soybean phospholipid oil at a high temperature of 60-90 ℃, adding 2-3 parts by weight of reducing sugar into a melted reaction pot and stirring until the materials are melted, keeping the temperature at 50 ℃ after reacting to be brown, heating and melting 18-20 parts by weight of oil, mixing the materials obtained in the previous step with the materials, and stirring uniformly to form a mixed solution, namely the coating liquid.

In some embodiments, the soybean phospholipid oil is a solid paste, consisting essentially of lecithin, cephalin, inositol phospholipid, phosphatidylserine, phosphatidic acid.

In some embodiments, the reducing sugar is one or more of glucose, fructose, galactose, lactose, xylose.

In some embodiments, the grease is a fatty acid having greater than or equal to 14 carbon atoms.

In some embodiments, the grease is one or more of stearic acid, glyceryl tristearate, glyceryl tripalmitate, coconut oil, hydrogenated palm oil, soy wax and carnauba wax, hydrogenated soybean oil, eicosanoic acid, iso-oleic acid, myristic acid, lauric acid, palmitic acid.

The temperature of the grease can be heated to 50-80 ℃ to be melted.

The technical principle of the rumen bypass coating preparation of the invention is as follows:

The components are made of grease to facilitate molding, otherwise the solution from the soybean lecithin oil will adhere.

Maillard reactions are a complex class of chemical reactions between amino compounds and carbonyl compounds (e.g., reducing sugars, lipids, aldehydes, ketones, etc.). And phosphatidylethanolamine contained in phosphatides (soybean phosphatides and lecithins) in the soybean phosphatide oil can generate Maillard reaction with reducing sugar, and has higher oxidation resistance and free radical scavenging capability. Therefore, the embodiment of the invention utilizes Maillard reaction between phospholipid and reducing sugar to produce a product with high rumen bypass rate and good intestinal release effect.

In addition, lecithin in the soybean lecithin oil has the advantages of improving the digestibility of fat-free nutrients such as fat-soluble vitamins, controlling the running and absorption of cholesterol, providing energy, having antioxidant property, improving the metabolic function of an organism, improving the activity of immune cells and enhancing the disease resistance and stress resistance.

Example 1

1) The preparation of the core-spun material adopts the medium-temperature alpha-amylase in the enzyme preparation feed as the active ingredient in the core-spun material. The amylase is in powder form. For the convenience of coating, talcum powder is used as a core material auxiliary material, and the specific operation is that 85 parts of alpha-amylase powder is taken, 15 parts of talcum powder is added, and 10 parts of absolute ethyl alcohol is added for uniform mixing. The mixed materials are rotated and extruded through a screen mesh with the diameter of 1.0mm, are rounded for 5 minutes by a rounding machine, are dried and cooled to room temperature, and particles with the diameter of 20-30 meshes are selected to be reserved as core materials. The core material (particles) thus prepared is shown in fig. 1.

2) Heating 18 parts of hydrogenated palm oil to 70 ℃ for melting, adding 2 parts of xylose after heating and melting 9 parts of soybean lecithin oil, mixing and stirring the soybean lecithin oil and the xylose at 80 ℃ until the material is brown yellow, and mixing the material and the melted hydrogenated palm oil to obtain the coating liquid.

3) The mass of the core-spun material and the dry matter of the coating liquid is 7:3, and the weight of the core-spun material and the coating liquid is used for feeding. And (3) carrying out bottom coating by adopting a fluidization coating machine, cooling to room temperature after coating material spraying is finished, and sieving to avoid adhesion. Sieving with 15-25 mesh sieve to obtain rumen bypass coating preparation. The prepared rumen bypass coating preparation (particles) is shown in fig. 2, and fig. 3 shows a state that the prepared rumen bypass coating preparation (particles) is soaked in water, and is basically not dissolved after being soaked for 16 hours, so that the coating liquid has a good coating effect on a core material, and indirectly shows that the coating preparation is not easy to be degraded by rumen microorganisms in the rumen, and is particularly important for improving the rumen bypass rate.

Example 2

1) The core material was prepared as in example 1.

2) Heating 18 parts of hydrogenated palm oil to 70 ℃ for melting, heating 9 parts of soybean lecithin oil for melting, adding 2 parts of xylose, mixing soybean lecithin oil and xylose at 90 ℃ and stirring until the material is brown yellow, and mixing the material with the melted hydrogenated palm oil to obtain the coating liquid.

3) The weight of the core-spun material and the weight of the coating liquid are fed by adopting the dry mass of the core-spun material and the coating liquid of 6:4. And (3) carrying out bottom coating by adopting a fluidization coating machine, cooling to room temperature after coating material spraying is finished, and sieving to avoid adhesion. Sieving with 15-25 mesh sieve to obtain rumen bypass coating preparation.

Example 3

1) The core material was prepared as in example 1.

2) Heating 18 parts of hydrogenated palm oil to 80 ℃ for melting, adding 2 parts of xylose after heating and melting 9 parts of soybean lecithin oil, mixing and stirring the soybean lecithin oil and the xylose at 80 ℃ until the material is brown yellow, and mixing the material and the melted hydrogenated palm oil to obtain the coating liquid.

3) The weight of the core-spun material and the weight of the coating liquid are 5:5. And (3) carrying out bottom coating by adopting a fluidization coating machine, cooling to room temperature after coating material spraying is finished, and sieving to avoid adhesion. Sieving with 15-25 mesh sieve to obtain rumen bypass coating preparation.

Example 4

1) The core material was prepared as in example 1.

2) Heating 20 parts of soybean wax to 60 ℃ to melt, heating 10 parts of soybean phospholipid oil to melt, adding 3 parts of fructose, mixing soybean phospholipid oil and fructose at 70 ℃, stirring until the materials are brown yellow, and mixing the materials with the melted soybean wax to obtain the coating liquid.

3) The weight of the core-spun material and the weight of the coating liquid are fed by adopting the dry mass of the core-spun material and the coating liquid of 6:5. And (3) carrying out bottom coating by adopting a fluidization coating machine, cooling to room temperature after coating material spraying is finished, and sieving to avoid adhesion. Sieving with 15-25 mesh sieve to obtain rumen bypass coating preparation.

Example 5

1) The core material was prepared as in example 1.

2) Heating 20 parts of soybean wax to 50 ℃ for melting, heating 10 parts of soybean phospholipid oil for melting, adding 3 parts of fructose, mixing soybean phospholipid oil and fructose at 60 ℃, stirring until the materials are brown yellow, and mixing the materials with the melted soybean wax to obtain the coating liquid.

3) The weight of the core-spun material and the weight of the coating liquid are fed by adopting the mass of the dry matters of the core-spun material and the coating liquid to be 7:4. And (3) carrying out bottom coating by adopting a fluidization coating machine, cooling to room temperature after coating material spraying is finished, and sieving to avoid adhesion. Sieving with 15-25 mesh sieve to obtain rumen bypass coating preparation.

Example 6

1) The core material was prepared as in example 1.

2) Heating 18 parts of stearic acid to 70 ℃ to melt, heating 9 parts of soybean phospholipid oil to melt, adding 2 parts of xylose, mixing soybean phospholipid oil and xylose at 60 ℃ and stirring until the material is brown yellow, and mixing the material with the melted stearic acid to obtain the coating liquid.

Comparative example 1

1) The core material was prepared as in example 1.

2) 20 Parts of hydrogenated vegetable oil is taken and heated to 70 ℃ to melt, so as to obtain coating liquid.

3) The weight of the core-spun material and the weight of the coating liquid are fed by adopting the dry mass of the core-spun material and the coating liquid of 6:4. And (3) carrying out bottom coating by adopting a fluidization coating machine, cooling to room temperature after coating material spraying is finished, and sieving to avoid adhesion. Sieving for 15-25 days to obtain rumen bypass coating preparation.

Comparative example 2

1) The core material was prepared as in example 1.

2) Heating 18 parts of hydrogenated palm oil to 70 ℃ to melt, and uniformly mixing 9 parts of soybean phospholipid oil after heating to melt to obtain coating liquid.

Comparative example 3

1) The core material was prepared as in example 1.

2) Heating 10 parts of hydrogenated palm oil to 70 ℃ for melting, adding 2 parts of xylose after heating and melting 8 parts of soybean lecithin oil, mixing and stirring the soybean lecithin oil and the xylose at 80 ℃ until the material is brown yellow, and mixing the material and the melted hydrogenated palm oil to obtain the coating liquid.

Comparative example 4

1) The core material was prepared as in example 1.

2) 25 Parts of hydrogenated palm oil are taken, the temperature is heated to 70 ℃ to melt, 8 parts of soybean lecithin oil is heated to melt, 2 parts of xylose is added, the soybean lecithin oil and the xylose are mixed and stirred at 80 ℃ until the materials are brown yellow, and the materials are mixed with the melted hydrogenated palm oil to obtain the coating liquid.

Comparative example 5

1) The core material was prepared as in example 1.

2) Heating 18 parts of hydrogenated palm oil to 70 ℃ for melting, adding 2 parts of xylose after heating and melting 4 parts of soybean lecithin oil, mixing and stirring the soybean lecithin oil and the xylose at 70 ℃ until the material is brown yellow, and mixing the material and the melted hydrogenated palm oil to obtain the coating liquid.

3) The weight of the core-spun material and the weight of the coating liquid are fed by adopting the mass of the dry matters of the core-spun material and the coating liquid to be 5:4. And (3) carrying out bottom coating by adopting a fluidization coating machine, cooling to room temperature after coating material spraying is finished, and sieving to avoid adhesion. Sieving with 15-25 mesh sieve to obtain rumen bypass coating preparation.

Comparative example 6

1) The core material was prepared as in example 1.

2) Heating 18 parts of hydrogenated palm oil to 70 ℃ for melting, heating 16 parts of soybean lecithin oil for melting, adding 2 parts of xylose, mixing soybean lecithin oil and xylose at 70 ℃, stirring until the material is brown yellow, and mixing the material with the melted hydrogenated palm oil to obtain the coating liquid.

Comparative example 7

1) The core material was prepared as in example 1.

2) Heating 18 parts of hydrogenated palm oil to 70 ℃ for melting, adding 1 part of xylose after heating and melting 8 parts of soybean lecithin oil, mixing and stirring the soybean lecithin oil and the xylose at 70 ℃ until the material is brown yellow, and mixing the material and the melted hydrogenated palm oil to obtain the coating liquid.

Comparative example 8

1) The core material was prepared as in example 1.

2) Heating 18 parts of hydrogenated palm oil to 70 ℃ for melting, adding 5 parts of xylose after heating and melting 8 parts of soybean lecithin oil, mixing and stirring the soybean lecithin oil and the xylose at 70 ℃ until the material is brown yellow, and mixing the material and the melted hydrogenated palm oil to obtain the coating liquid.

Comparative example 9

1) The core material was prepared as in example 1.

2) Heating 18 parts of hydrogenated palm oil to 80 ℃ for melting, adding 2 parts of xylose after heating and melting 8 parts of soybean lecithin oil, mixing and stirring the soybean lecithin oil and the xylose at 50 ℃ until the material is brown yellow, and mixing the material and the melted hydrogenated palm oil to obtain the coating liquid.

Comparative example 10

1) The core material was prepared as in example 1.

2) Heating 20 parts of hydrogenated palm oil to 70 ℃ for melting, adding 2 parts of xylose after heating and melting 8 parts of soybean lecithin oil, mixing and stirring the soybean lecithin oil and the xylose at 100 ℃ until the material is brown yellow, and mixing the material and the melted hydrogenated palm oil to obtain the coating liquid.

The rumen bypass effect and intestinal release rate of each example and comparative example are measured as shown in Table 1 below.

TABLE 1 rumen bypass effect and intestinal Release Rate measurement results

As can be seen from the comparison of each example with comparative examples 1 and 2, comparative example 1 only adds one hydrogenated vegetable oil, and comparative example 2 does not use reducing sugar, which means that no Maillard reaction can occur without using soybean phospholipid oil or reducing sugar, resulting in a great reduction of the rumen bypass rate for 16 hours and the small intestine release rate for 24 hours, and poor rumen bypass rate and intestinal release effect.

As is evident from the comparison of examples with comparative examples 3 and 4, the use of 10 parts and 25 parts of grease (hydrogenated palm oil) respectively, the use of lower and higher amounts of grease resulted in poor material blocking and intestinal release.

As is clear from comparison of each example with comparative example 5 and comparative example 6, the use of 4 parts and 10 parts of soybean lecithin oil respectively, the lower and higher amounts of soybean lecithin oil resulted in poor intestinal release effect and material adhesion.

As is evident from the comparison of examples with comparative examples 7 and 8, the use of 1 part of xylose and 5 parts of xylose, respectively, resulted in either a lower xylose dosage or a higher xylose dosage, which resulted in either a failure to form Maillard reaction or an excessive reaction, which resulted in failure of intestinal tract to release.

As can be seen from the comparison of examples 9 and 10, the high temperature melting temperatures of soybean phospholipid oil and xylose are respectively 50 ℃ and 100 ℃, and the temperature of the reduction reaction is higher or lower, so that the Maillard reaction cannot be moderate, and oxidation-resistant beneficial factors cannot be formed or harmful substances cannot be excessively formed.

The measuring method comprises the following steps:

Rumen bypass rate was determined using a semi-in nylon bag method.

Nylon bags with the aperture of 40um and the size of 12cmx10cm are numbered in advance, washed, dried at 65 ℃ for 48 hours and then weighed. 5g of the sample was weighed with an analytical balance and placed in a nylon bag of known weight. Nylon bags are put in, namely nylon ropes are used for binding nylon bags filled with samples on different iron chains, each iron chain is bound with 12 nylon bags at most, and 3 nylon bags at each time point of each sample are used as parallel. According to the principle of putting and taking out at the same time, putting an iron chain bound with a nylon bag into the rumen sac through a rumen fistula, tying one end of an iron rod on the fistula cover through a nylon rope, putting one iron chain in each fistula, and taking out the nylon bag after fermenting for the same time in vivo. And (3) cleaning, namely immediately flushing the taken nylon bag under running water until the water flowing out of the nylon bag is clear and bright, and lightly squeezing out excessive water by hands after cleaning. And (3) drying, namely drying the nylon bag for 48 hours at the temperature of 45 ℃, taking out, weighing, and transferring the nylon bag into a sealed bag for subsequent analysis.

Rumen degradation rate = (weight of sample in bag-weight after digestion at each time point of rumen)/weight of sample in bag x 100%.

Gastric and intestinal digestion, pepsin and pancreatin digestion tests were performed according to the modified three-step in vitro method suggested by Gargallo et al. The rumen stability test method is adopted to prepare a residue sample after digestion for 16 hours, 0.5g of residue after in vitro rumen degradation for 16 hours is weighed into a fiber bag (ANKOM-FN 57), and the fiber bag is filled into culture bottles after sealing, and each bottle is filled with 30 fiber bags at most. Each group was set with 3 replicates, nine groups, 27 fiber bags. 2L of hydrochloric acid solution with pH=1.9 containing 1g/L pepsin (P-7000, sigma) was added to the flask, and the solution was preheated in advance. The flasks were placed in an in vitro simulated fermentation incubator (Daisy II, ankom, USA) and spun at 39℃for 1h. 2L of pre-warmed 0.5mol/L phosphate buffer containing 3g/L trypsin and 50mg/L thymol was added and the flask was placed in an in vitro simulated fermentation incubator (Daisy II, ankom, USA) and spun at 39℃for 24h. The fiber bag is taken out and washed to be clear with water, and is baked in a 65 ℃ oven for 48 hours to constant weight.

Small intestine degradation rate= (weight of sample before small intestine digestion-weight of sample after small intestine digestion)/weight of sample before digestion x 100%.

Industrial applicability

The rumen bypass coating preparation provided by the embodiment of the invention is mainly applied to the field of agriculture and livestock, can be used for preparing feed additives for ruminants and preparing medicines for ruminants, and consists of rumen bypass nutrients, soybean phospholipid oil, grease, reducing sugar, an adhesive and a dispersing agent, wherein after the nutrients are granulated by adopting a granulating, ball-throwing and rounding device, the nutrients are coated in a bottom spraying mode by adopting a fluidized bed granulating and coating machine. And the Maillard reaction product is generated by utilizing the Maillard reaction, and the Maillard product and grease are mixed and sprayed, so that the degradation in the small intestine is promoted and the release rate of the small intestine is improved on the premise of ensuring high rumen bypass rate.

The foregoing description of embodiments of the invention has been presented for purposes of illustration and description, and is not intended to be exhaustive or limited to the embodiments disclosed. Many modifications and variations will be apparent to those of ordinary skill in the art without departing from the scope and spirit of the various embodiments described. The terminology used herein was chosen in order to best explain the principles of the embodiments, the practical application, or the technical improvements in the marketplace, or to enable others of ordinary skill in the art to understand the embodiments disclosed herein.

Claims

1. The ruminant rumen bypass coating preparation comprises a core material and a coating, and is characterized in that a coating liquid for preparing the coating comprises the following components in parts by weight:

8-10 parts of soybean lecithin oil;

2-3 parts of reducing sugar;

18-20 parts of grease;

the coating liquid is prepared by the following method:

2. The rumen bypass coating preparation according to claim 1, wherein the soybean phospholipid oil is a solid paste mainly containing lecithin, cephalin, inositol phospholipid, phosphatidylserine, phosphatidic acid.

3. The rumen bypass coating formulation of claim 1, wherein the reducing sugar is one or more of glucose, fructose, galactose, lactose, xylose.

4. The rumen bypass coating formulation of claim 1, wherein the lipid is a fatty acid having greater than or equal to 14 carbon atoms.

5. The rumen bypass coating formulation of claim 4, wherein the lipid is one or more of stearic acid, glyceryl tristearate, glyceryl tripalmitate, coconut oil, hydrogenated palm oil, soy wax and carnauba wax, hydrogenated soy oil, eicosanoic acid, iso-oleic acid, myristic acid, lauric acid, palmitic acid.

6. A method of preparing the rumen bypass coating formulation according to any one of claims 1 to 5, comprising:

7. The preparation method according to claim 6, wherein the rumen bypass coating preparation is obtained by cooling to room temperature after spraying the coating material and sieving with a 15-25 mesh sieve.

8. A device for realizing the preparation method as claimed in claim 6 or 7, which is characterized by comprising a multifunctional fluid bed granulating and coating machine, wherein the material yield is more than 99%, the water content of a finished product is less than 0.5%, fluidization and air flow conveying are combined through bottom spraying operation, homodromous liquid spraying and nozzle embedding are adopted, the probability of particle fluidization passing through spray mist particles is high, and the particles are highly dense in a liquid spraying area.

9. Use of the rumen bypass coating formulation according to any one of claims 1 to 5 in the preparation of a feed additive for ruminants.

10. Use of the rumen bypass coating formulation of any one of claims 1 to 5 in the manufacture of a medicament for ruminants.