CN101431903B - Method for preparing ruminally protected choline - Google Patents

Abstract

The present invention relates to a method for preparing ruminally protected choline, which is not degraded by microorganisms, pH and water in the rumen, and thus can be stably delivered to and absorbed in the lower digestive tract. In particular, the present invention relates to a method for preparing double-coated ruminally protected choline comprising the steps of: preparing carrier-immobilized choline by immobilizing concentrated liquid choline on a carrier and then removing water from the immobilized choline; (b) preparing a first encapsulated choline by encapsulating the carrier-immobilized choline with a binder/coating material; (c) screening encapsulated choline having a size of 0.5-2.0mm from the first encapsulated choline; and (d) further encapsulating the screened encapsulated choline with any one or a combination of two or more selected from the group consisting of extremely hydrogenated oils or fats, and fatty acids having a melting point of higher than 40 ℃.

Description

Process for preparing rumination-protected choline

technical field

The present invention relates to a method for preparing rumination-protected choline, which is not degraded by microorganisms, pH and water in the rumen, and thus can be stably delivered to and absorbed in the lower digestive tract. Specifically, the present invention relates to a method for preparing double-coated ruminally protected choline comprising the steps of: (a) preparing the carrier by immobilizing concentrated liquid choline on the carrier and then removing water from the immobilized choline The choline of immobilization; (b) prepare the choline of encapsulation for the first time by the choline of immobilization of encapsulation carrier with adhesive/coating material; (c) screen the choline with 0.5-2.0mm from the choline of first encapsulation and (d) further encapsulate the screened encapsulated choline using any one or a combination of two or more selected from the group consisting of extremely hydrogenated oils or fats, and having a value higher than 40 Fatty acids with a melting point of °C.

Background technique

Choline is known to be an essential component in some livestock studies, but the choline requirement for lactating dairy cows remains unestablished. Also, most of choline is degraded in the rumen of ruminants, so there is a need to develop technology to protect choline from degradation so that choline can pass through the rumen and be absorbed and used in the small intestine.

The lactational response to choline is similar to that to methionine, an essential amino acid, and typical symptoms of choline deficiency include muscle weakness, development of fatty liver, renal hemorrhage, and similar symptoms.

Choline has been found to be the limiting nutritional factor in dairy cows through studies with choline injections. Also, in the case of choline deficiency, fatty liver occurs due to fatty mechanism and abnormal secretion mechanism of the liver, and when the injection of choline increases, the movement of fatty acid increases, thereby leading to a decrease in the occurrence of fatty liver.

It has been reported that choline affects blood sugar, plasma cholesterol, serum insulin and serum NEFA (non-esterified fatty acids), which greatly contributes to the improvement of milk production, milk composition, weight gain and carcass traits, but the addition of choline chloride to the feed Bases are mostly degraded in the rumen.

Ruminant microorganisms degrade phosphatidylcholine in feed to choline and phosphodiglycerides, and also degrade choline by converting choline to methane via trimethylamine as an intermediate metabolite. As a result, less than 1% of the choline in the diet is known to reach the small intestine.

Choline is an essential nutrient in livestock animals, but its effect cannot be observed in ruminants because it is degraded in the rumen. For this reason, attempts have been made for a long time to prevent the degradation of the choline component in the rumen.

For example, an encapsulated choline composition was reported for enhancing weight gain in ruminants (US 5,807,594). However, the application of the choline composition as an actual product will incur higher costs due to various materials used as an encapsulating agent for the choline composition.

Meanwhile, US5,496,571 discloses encapsulated choline compositions that can be fed to lactating cows, and US5,190,775 discloses that biologically active substances such as choline can be prevented from being degraded in the rumen when fed through a hydrophobic coating. However, said patent does not suggest a specific method of preparing the composition, and a simple coating or encapsulation with a hydrophobic substance according to said patent does not provide sufficient protection of the active substance from degradation by ruminant microorganisms in the rumen.

Furthermore, WO94/15480 discloses feeding ruminants an encapsulated choline composition to increase milk production. However, in this patent, a hydrophobic coating is also used and there is no specific mention of the method used to prepare the choline composition. Furthermore, WO96/08168 discloses a ruminant feed additive which can be prepared by encapsulating a liquid choline composition in a carrier and coating the composition with a fatty acid or fatty acid salt. However, its disadvantage is that when liquid choline is coated with fatty acid salt, fatty acid salt can react with carbon dioxide in the air.

In addition, a lipid coating (WO03/059088A1) and a double coating (US2005/0019413A1) to stabilize choline in the rumen are known. However, there is a disadvantage that the method of forming the coating is complicated.

As mentioned above, many patents are published related to choline compositions for enhancing weight gain and milk production, and most mention that choline is coated with a specific protective substance so that it is not degraded in the rumen. However, these patents do not specifically disclose methods for preparing choline compositions.

Also, among the rumination-protected choline products currently available on the market, there are products prepared by mixing choline with a protecting substance and subjecting the mixture to a cooling spraying step. In this case, however, production site costs are high and installation costs are high, and very cold air having a temperature of -60° C. is used, resulting in an increase in production costs.

In addition, since the product is prepared using a rapid cooling process, macro pores may be formed in the product, and operating conditions for product production are also complicated. In addition, the choline located on the surface of the rumination protected choline product cannot be protected, resulting in reduced product efficacy.

Therefore, there is an urgent need to develop techniques for preparing coated choline compositions that are stable against ruminant degradation and that can be fed to ruminants.

Therefore, the inventors of the present invention have made great efforts to solve the problem of choline degradation in the rumen of ruminants. As a result, the inventors of the present invention found that rumination-protected choline prepared by the following method was stably delivered to and absorbed in the lower digestive tract without being degraded by microorganisms, pH, and water in the rumen, thereby completing the present invention. Invention: immobilizing choline on a carrier and removing water from the immobilized choline, encapsulating the non-aqueous choline with a hydrophobic substance, and then further encapsulating said encapsulated choline with a substance selected from the group consisting of Extremely hydrogenated oils or fats, and fatty acids with a melting point above 40°C.

Contents of the invention

The object of the present invention is to provide a process for the preparation of double-coated ruminally protected choline which is not degraded by microorganisms, pH and water in the rumen of ruminants.

In order to achieve the above object, the present invention provides a method for preparing double-coated ruminant-protected choline, the method comprising the following steps: (a) by immobilizing concentrated liquid choline on a carrier and then Remove moisture to prepare the choline immobilized by the carrier; (b) prepare the choline encapsulated for the first time by using the choline immobilized by the adhesive/coating material encapsulation carrier; (c) screen the choline with Encapsulated choline with a size of 0.5-2.0 mm; and (d) further encapsulating the screened encapsulated choline using any one or a combination of two or more selected from the group consisting of extremely hydrogenated oils or fats, and Fatty acids having a melting point above 40°C.

The above and other objects, features and embodiments of the present invention will be better understood from the following detailed description and appended claims.

Detailed ways

The present invention relates to a process for the preparation of double-coated ruminally protected choline, which is not degraded by microorganisms, pH and water in the rumen of ruminants, so that it can be stably delivered to and absorbed in the lower digestive tract .

According to the present invention, in order to prepare double-coated ruminant-protected choline, concentrated liquid choline is immobilized on a carrier, and then water is removed therefrom to prepare carrier-immobilized choline.

From this point of view, the concentrated liquid choline is preferably selected from one of the following groups or a mixture of two or more thereof, which group includes choline chloride, dihydrocholine citrate, tricholine citrate base and choline bitartrate. The carrier is preferably a water-insoluble carrier selected from the group consisting of silicon, cellulose, starch and zeolite. More preferably the support is silicon.

Then, the carrier-immobilized choline is encapsulated with a binder/coating material to produce granular or needle-shaped first-encapsulated choline. The packaging of the carrier-immobilized choline is achieved by mixing and stirring the immobilized choline with the binder/coating material in a mixer such as a ribbon mixer, conventional blender, high-speed blender or V-blender, followed by extrusion extruder or screw extruder to extrude the mixture. Here, the choline and the binder/coating material are preferably mixed with each other in a weight ratio of 1:00.1-2.0. Also, the porous plate in the extruder preferably has a size of 0.5-2.5 mm, and the extruding cylinder in the extruder is preferably kept at a temperature of 10-60°C.

Furthermore, the binder/coating material is preferably any one or a mixture of two or more selected from the group consisting of monovalent fatty acid salts, divalent fatty acid salts, and fatty acid. The monovalent fatty acid salt is preferably fatty acid sodium or fatty acid potassium, and the divalent fatty acid salt is preferably selected from the group consisting of beeswax, PEG, surfactants, calcium stearate, zinc stearate and magnesium stearate. The fatty acid having a melting point above 40°C is preferably selected from the group consisting of hydrogenated oils, extremely hydrogenated oils, palmitic acid and stearic acid. More preferred are hydrogenated oils.

Then, the encapsulated choline having a particle size of 0.5-2.0 mm was screened from the first encapsulated choline. The screened encapsulated choline is further encapsulated with any one selected from the group or a combination of two or more, including extremely hydrogenated oils and fats, and fatty acids with a melting point higher than 40° C., thereby preparing a double-coated ruminant Protected choline.

The total amount of double coating content in the finally prepared double coating ruminant protected choline is preferably 15-55% (w/w) compared to the control.

The double-coated ruminant-protected choline prepared according to the present invention has the advantage that it does not exhibit the formation of macropores and has a uniform shape, which is a problem in products prepared according to existing commercial cooling spray processes .

Furthermore, products prepared according to the cooling spray process have the disadvantage that the choline is localized on the surface of the particles, thus reducing the stability in the rumen, whereas the double-coated rumen-protected choline prepared according to the present invention has the advantage that , since the choline-containing product after the first coating (encapsulation) step is further subjected to the second coating (encapsulation) step, the possibility of choline distribution on the surface of the product is reduced, so most of the choline contained in the product Can be kept stable. In addition, the present invention does not require the use of the refrigerant used in the cooling spray process, thereby reducing the production cost, and requires very low production site and installation costs, thereby reducing the production cost of the product.

When feeding ruminants with choline without rumination protection, most of the choline without protection will be degraded in the rumen, making the effect of feeding choline unpredictable and resulting in choline waste. On the other hand, when the invented ruminant-protected choline with increased stability is used in the farming industry, it can increase the utilization of nutrients by ruminants, thereby enabling an increase in livestock production and showing increased effect. Therefore, the ruminant-protected choline of the present invention may contribute to the development of feed additives capable of producing functional livestock.

Example

Hereinafter, the present invention will be described in further detail with reference to Examples. However, it should be understood that these examples are for illustrative purposes only and do not limit the scope of the invention.

Example 1: Preparation of choline for double-coated ruminant protection

Concentrated liquid choline is immobilized on a carrier such as silicon, cellulose, starch, zeolite or the like, and then water is removed therefrom, thereby preparing carrier-immobilized choline.

Then, an adhesive/coating material such as beeswax, PEG, hydrogenated oil, or the like is added to the carrier-immobilized choline, and then mixed in a mixer such as a ribbon mixer, ordinary mixer, high-speed mixer, or V-type mixer. Mix and agitate the immobilized choline with the binder/coating material in a mixer or similar device. The mixture is extruded through an extruder or a screw extruder, thereby preparing first encapsulated (coated) choline. Here, the temperature of the extrusion barrel in the extruder or screw extruder is maintained at 10-60°C.

Among the first encapsulated cholines, encapsulated cholines having a size of 0.5-2.0 mm were screened. Add one or a combination of two or more selected from the group consisting of extremely hydrogenated oils or fats, fatty acids with a melting point higher than 40°C, such as hydrogenated rapeseed oil, hydrogenated corn Oil, hydrogenated cottonseed oil, hydrogenated peanut oil, hydrogenated palm kernel oil, hydrogenated palm oil, hydrogenated palm stearic acid, hydrogenated sunflower oil, hydrogenated rapeseed oil, palmitic and stearic acids. The mixture containing the first encapsulated choline is subjected to a second encapsulation step in an encapsulation apparatus such as a high speed mixer, ribbon mixer, roedige mixer or fluid bed processor. A double-coated rumination-protected choline was thus prepared.

test case 1

1 kg of liquid hydrogenated oil (primary encapsulation material; adhesive/coating material) was mixed with 5 kg of choline, and 1 kg of the mixture was extruded through a perforated plate with a pore diameter of 1.0 mm to prepare a primary encapsulation material . 1 kg of the first packaging material prepared is placed in a high-speed mixer, and then 670 g of any one or a combination of two or more selected from liquid hydrogenated oils and fatty acids with a melting point higher than 40° C. is added to the high-speed mixer. Mixer as the second encapsulation material, thereby preparing the second encapsulation material. Here, the amount of the second packaging material is 40.1% of the total weight of the final produced second packaging material.

test case 2

1 kg of liquid hydrogenated oil (primary encapsulation material; adhesive/coating material) was mixed with 5 kg of choline, and 1 kg of the mixture was extruded through a perforated plate with a pore diameter of 1.2 mm to prepare a primary encapsulation material . 1 kg of the first packaging material prepared is placed in a high-speed mixer, and then 670 g of any one or a combination of two or more selected from liquid hydrogenated oils and fatty acids with a melting point higher than 40° C. is added to the high-speed mixer. Mixer as the second encapsulation material, thereby preparing the second encapsulation material. Here, the amount of the second packaging material is 40.1% of the total weight of the final produced second packaging material.

Test case 3

1 kg of liquid hydrogenated oil (primary encapsulation material; adhesive/coating material) was mixed with 5 kg of choline, and 1 kg of the mixture was extruded through a perforated plate with a pore size of 1.5 mm to prepare a primary encapsulation material . 1 kg of the first packaging material prepared is placed in a high-speed mixer, and then 670 g of any one or a combination of two or more selected from liquid hydrogenated oils and fatty acids with a melting point higher than 40° C. is added to the high-speed mixer. Mixer as the second encapsulation material, thereby preparing the second encapsulation material. Here, the amount of the second packaging material is 40.1% of the total weight of the final produced second packaging material.

Test case 4

1 kg of liquid hydrogenated oil (primary encapsulation material; adhesive/coating material) was mixed with 5 kg of choline, and 1 kg of the mixture was extruded through a perforated plate with a pore diameter of 2.0 mm to prepare a primary encapsulation material . 1 kg of the first packaging material prepared is placed in a high-speed mixer, and then 670 g of any one or a combination of two or more selected from liquid hydrogenated oils and fatty acids with a melting point higher than 40° C. is added to the high-speed mixer. Mixer as the second encapsulation material, thereby preparing the second encapsulation material. Here, the amount of the second packaging material is 40.1% of the total weight of the final produced second packaging material.

Test case 5

1 kg of monovalent fatty acid (primary encapsulation material; adhesive/coating material) was mixed with 5 kg of choline, and 1 kg of the mixture was extruded through a perforated plate with a pore size of 1.0 mm to prepare a primary encapsulation material. 1 kg of the first packaging material prepared is placed in a high-speed mixer, and then 670 g of any one or a combination of two or more selected from liquid hydrogenated oils and fatty acids with a melting point higher than 40° C. is added to the high-speed mixer. Mixer as the second encapsulation material, thereby preparing the second encapsulation material. Here, the amount of the second packaging material is 40.1% of the total weight of the final produced second packaging material.

Test case 6

1 kg of PEG (primary encapsulating material; adhesive/coating material) was mixed with 5 kg of choline, and 1 kg of the mixture was extruded through a perforated plate having a pore diameter of 1.0 mm to prepare a primary encapsulating material. 1 kg of the first packaging material prepared is placed in a high-speed mixer, and then 670 g of any one or a combination of two or more selected from liquid hydrogenated oils and fatty acids with a melting point higher than 40° C. is added to the high-speed mixer. Mixer as the second encapsulation material, thereby preparing the second encapsulation material. Here, the amount of the second packaging material is 40.1% of the total weight of the final produced second packaging material.

Test case 7

1 kg of Tween 80 (primary encapsulation material; adhesive/coating material) was mixed with 5 kg of choline, and 1 kg of the mixture was extruded through a perforated plate with a pore size of 1.0 mm to prepare a primary encapsulation material . 1 kg of the first packaging material prepared is placed in a high-speed mixer, and then 670 g of any one or a combination of two or more selected from liquid hydrogenated oils and fatty acids with a melting point higher than 40° C. is added to the high-speed mixer. Mixer as the second encapsulation material, thereby preparing the second encapsulation material. Here, the amount of the second packaging material is 40.1% of the total weight of the final produced second packaging material.

Test case 8

1 kg of Span80 (primary packaging material; adhesive/coating material) was mixed with 5 kg of choline, and 1 kg of the mixture was extruded through a perforated plate having a pore size of 1.0 mm to prepare a primary packaging material. 1 kg of the first packaging material prepared is placed in a high-speed mixer, and then 670 g of any one or a combination of two or more selected from liquid hydrogenated oils and fatty acids with a melting point higher than 40° C. is added to the high-speed mixer. Mixer as the second encapsulation material, thereby preparing the second encapsulation material. Here, the amount of the second packaging material is 40.1% of the total weight of the final produced second packaging material.

Test case 9

1 kg of liquid hydrogenated oil (primary encapsulation material; adhesive/coating material) was mixed with 5 kg of choline, and 1 kg of the mixture was extruded through a perforated plate with a pore diameter of 1.2 mm to prepare a primary encapsulation material . 1 kg of the first packaging material prepared is placed in a high-speed mixer, and then 500 g of any one or a combination of two or more selected from liquid hydrogenated oils and fatty acids with a melting point higher than 40° C. is added to the high-speed mixer. Mixer as the second encapsulation material, thereby preparing the second encapsulation material. Here, the amount of the second packaging material is 33.3% of the total weight of the final produced second packaging material.

Test case 10

1 kg of liquid hydrogenated oil (primary encapsulation material; adhesive/coating material) was mixed with 5 kg of choline, and 1 kg of the mixture was extruded through a perforated plate with a pore diameter of 1.2 mm to prepare a primary encapsulation material . 1 kg of the first packaging material prepared is placed in a high-speed mixer, and then 350 g of any one or a combination of two or more selected from liquid hydrogenated oils and fatty acids with a melting point higher than 40° C. is added to the high-speed mixer. Mixer as the second encapsulation material, thereby preparing the second encapsulation material. Here, the amount of the second packaging material is 25.9% of the total weight of the final produced second packaging material.

Test case 11

1 kg of liquid hydrogenated oil (primary encapsulation material; adhesive/coating material) was mixed with 5 kg of choline, and 1 kg of the mixture was extruded through a perforated plate with a pore diameter of 1.2 mm to prepare a primary encapsulation material . 1 kg of the first packaging material prepared is placed in a high-speed mixer, and then 250 g of any one or a combination of two or more selected from liquid hydrogenated oils and fatty acids with a melting point higher than 40° C. is added to the high-speed mixer. Mixer as the second encapsulation material, thereby preparing the second encapsulation material. Here, the amount of the second packaging material is 20.2% of the total weight of the final produced second packaging material.

Test case 12

1 kg of liquid hydrogenated oil (primary encapsulation material; adhesive/coating material) was mixed with 5 kg of choline, and 1 kg of the mixture was extruded through a perforated plate with a pore diameter of 1.2 mm to prepare a primary encapsulation material . 1 kg of the first packaging material prepared is placed in a high-speed mixer, and then 150 g of any one or a combination of two or more selected from liquid hydrogenated oils and fatty acids with a melting point higher than 40° C. is added to the high-speed mixer. Mixer as the second encapsulation material, thereby preparing the second encapsulation material. Here, the amount of the second packaging material is 13.0% of the total weight of the final produced second packaging material.

Test case 13

1 kg of liquid hydrogenated oil (primary encapsulation material; adhesive/coating material) was mixed with 5 kg of choline, and 1 kg of the mixture was extruded through a perforated plate with a pore diameter of 1.2 mm to prepare a primary encapsulation material . 1 kg of the first packaging material prepared is placed in a high-speed mixer, and then 50 g of any one or a combination of two or more selected from liquid hydrogenated oils and fatty acids with a melting point higher than 40° C. is added to the high-speed mixer. Mixer as the second encapsulation material, thereby preparing the second encapsulation material. Here, the amount of the second packaging material is 4.8% of the total weight of the final produced second packaging material.

Test case 14

2 kg of liquid hydrogenated oil (primary encapsulation material; adhesive/coating material) was mixed with 4 kg of choline, and 1 kg of the mixture was extruded through a perforated plate with a pore size of 2.0 mm to prepare a primary encapsulation material . 1 kg of the first packaging material prepared is placed in a high-speed mixer, and then 670 g of any one or a combination of two or more selected from liquid hydrogenated oils and fatty acids with a melting point higher than 40° C. is added to the high-speed mixer. Mixer as the second encapsulation material, thereby preparing the second encapsulation material. Here, the amount of the second packaging material is 40.1% of the total weight of the final produced second packaging material.

Test case 15

3 kg of liquid hydrogenated oil (primary encapsulation material; adhesive/coating material) was mixed with 3 kg of choline, and 1 kg of the mixture was extruded through a perforated plate with a pore size of 2.0 mm to prepare a primary encapsulation material . 1 kg of the first packaging material prepared is placed in a high-speed mixer, and then 670 g of any one or a combination of two or more selected from liquid hydrogenated oils and fatty acids with a melting point higher than 40° C. is added to the high-speed mixer. Mixer as the second encapsulation material, thereby preparing the second encapsulation material. Here, the amount of the second packaging material is 40.1% of the total weight of the final produced second packaging material.

Example 2: In vitro loss of choline for rumination protection

The in vitro loss of choline of each double-coated layer prepared in Test Examples 1-15 was measured in the following manner, and the results of the measurement are shown in Table 1 below.

In order to determine the in vitro stability of each double-coated choline prepared in Test Examples 1-15, each prepared ruminant-protected choline was placed in a test tube, and distilled water was added to the test tube to prepare 5% choline aqueous solution. The aqueous solution was stirred at 40°C ± 0.1°C for 2 hours using a JEIO TECH MC-11 multi-stirrer.

The stirred aqueous solution was filtered through No. 3 Whatman paper to collect only the solids. The solids were dried at 45°C ± 0.1°C for at least 12 hours to completely remove residual moisture.

The dried sample was cooled to room temperature in a Sanpla Dry Keeper, and 300 mg of the cooled sample was completely dissolved in a mixed solvent of chloroform and ethanol (1:1 v/v). Use 5% K2CrO4 as indicator and solution 0.1N _AgNO3 as titrant to determine the content of choline in the solution.

The measurement results are shown in Table 1 below. As shown in Table 1, there was little or no change in in vitro choline loss according to changes in the well size of the multiwell plate (Test Examples 1-4).

Regarding the type of primary encapsulating material, compared with the case of using Tween 80 or Span80, the in vitro loss of choline was shown to be reduced when monovalent fatty acid or divalent fatty acid PEG was used as the primary encapsulating material. As a result, it was observed that the use of liquid hydrogenated oil as the primary encapsulation material resulted in reduced loss of choline in vitro compared to the use of monovalent fatty acid, divalent fatty acid PEG, Tween 80 or Span80 (Test Examples 5-8).

With regard to the amount of secondary encapsulating material added, it can be seen that an increase in the amount of secondary encapsulating material added leads to a decrease in in vitro loss of choline (Test Examples 9-13).

Furthermore, with regard to the ratio of primary encapsulating material to choline, it was observed that an increase in the ratio of primary encapsulating material to choline resulted in a decrease in the loss of choline in vitro (test examples 14 and 15).

Table 1

test case In vitro loss (%) 1 10.41 2 7.91 3 9.57 4 10.75 5 28.71 6 45.11 7 52.74 8 54.46 9 21.95 10 31.55 11 42.20 12 49.50 13 62.24 14 4.57 15 4.36

As a result, it can be found that by selecting liquid hydrogenated oil as the first encapsulation material, mixing choline with the first encapsulation material at a ratio of 1:0.5 or 1:1, subjecting the mixture to the first encapsulation step and using Zeng's Quantities of secondary encapsulation material The double-coated ruminant-protected choline prepared by subjecting the first encapsulation material to a second encapsulation step was shown to minimize in vitro loss.

Example 3: Feeding Test of Choline for Rumination Protection

In vivo feeding tests were performed on the rumination-protected choline prepared in Test Example 15, which showed low in vitro loss in the test of Example 2 and had a suitable size and high choline content.

For the dairy cow feeding test using rumination-protected choline, 10 cows in the middle lactation period were selected as test animals and given a total mixed ration of a sufficient amount of the compound feed and fed heavily twice a day at 12-hour intervals. Furthermore, rumination-protected choline (25% choline content) was additionally fed to the cows in an amount of 40 g per cow (based on 10 g choline) when fed the total mixed ration. Cows were lactated twice daily and milk production was measured. Milk samples were taken from produced milk, and the milk composition and somatic cell number in the milk samples were determined using an automatic analyzer (LactoScopeR, MK2, DeltaInstruments, The Netherlands).

The measurement results are shown in Table 2 below. As shown in Table 2, in the case where rumination-protected choline was fed to the cow, the daily milk production of the cow increased by 9.9% from 29.2 kg before feeding to 32.1 kg after feeding, milk fat , milk protein, lactose and total solids also increased by 7.9%, 10.4%, 12.7% and 10.1%, respectively. Moreover, the number of somatic cells in the samples decreased by 61.9% from 167,000 cells/ml to 103,000 cells/ml due to feeding with rumination-protected choline, indicating that rumination-protected choline is effective in improving milk quality. In addition, the total amount of choline content in the samples increased by approximately 48.8% over the control, from 44.85 mg/ml to 66.75 mg/ml, indicating that rumination-protected choline enables the production of choline-enriched milk.

Table 2

Milk production (kg/day) Creamy milk protein lactose total solids Somatic cell number (10 ³ /me) Choline (mg/me) before feeding 29.2 3.43% 2.79% 4.49% 11.57% 167 44.85 after feeding 32.1 3.70% 3.08% 5.06% 12.74% 103 66.75

content change 109.9% 107.9% 110.4% 112.7% 110.1% 61.9% 148.8%

Industrial Applicability

As described in detail above, the present invention provides a process for the preparation of double-coated ruminant protected choline. The ruminant-protected choline prepared according to the present invention is not degraded by microorganisms, pH and water in the rumen, and thus can be stably delivered to and absorbed in the lower digestive tract. Also, when it is used to feed ruminant livestock, it can increase the utilization of nutrients by ruminant livestock, thereby increasing the yield of livestock, making it possible to produce functional livestock that produce choline-rich milk. In addition, the method for preparing rumination-protected choline of the present invention can be used to protect various components from being degraded by rumination, and can be used in the production of various new feed additives.

Although the invention has been described in detail with reference to specific features, it will be apparent to those skilled in the art that the description is for the preferred embodiment only and does not limit the scope of the invention. Therefore, the actual scope of the present invention will be defined by the appended claims and their equivalents.

Claims (16)

1. method for preparing the ruminally protected choline of two coatings, this method comprises the following steps:

(a) from fixing choline, remove moisture on the carrier then and prepare the fixing choline of carrier through the liquid choline that concentrates is fixed on;

(b) choline that bonding agent/coating material and carrier is fixing mixes in blender and stirs, and encapsulation prepares the encapsulated choline first time in the extruding machine then;

(c) screen encapsulated choline from the first time the encapsulated choline with 0.5-2.0mm size; With

(d) use and to be selected from down the encapsulated choline that organize any or two or more combination further encapsulate screening, its group comprises the oil or the fat of deep hydrogenation, and the aliphatic acid with the fusing point that is higher than 40 ℃.

2. the method for the choline that the two coatings of preparation according to claim 1 are ruminally protected; It is characterized in that; The liquid choline that concentrates is to be selected from down a kind of in organizing or two or more mixture wherein, and its group comprises Choline Chloride, dihydrogen citrate choline, citric acid three choline and choline bitartrate.

3. the method for the choline that the two coatings of preparation according to claim 1 are ruminally protected is characterized in that carrier is the water insoluble carrier that is selected from down group, and its group comprises silicon, cellulose, starch and zeolite.

4. the method for the choline that the two coatings of preparation according to claim 1 are ruminally protected; It is characterized in that; Binding agent/coating material is selected from down any of group or two or more mixture; Its group comprises unit price soap, divalence soap, and the aliphatic acid with the fusing point that is higher than 40 ℃.

5. the method for the choline that the two coatings of preparation according to claim 4 are ruminally protected is characterized in that unit price soap is sodium soap or aliphatic acid potassium.

6. the method for the choline that the two coatings of preparation according to claim 4 are ruminally protected is characterized in that, at least a during divalence soap is selected from down and organizes, and its group comprises surfactant, calcium stearate, zinc stearate and dolomol.

7. the method for the choline that the two coatings of preparation according to claim 4 are ruminally protected is characterized in that the aliphatic acid with the fusing point that is higher than 40 ℃ is selected from down group, and its group comprises hydrogenated oil and fat, deep hydrogenation oil, palmitic acid and stearic acid.

8. the method for the choline that the two coatings of preparation according to claim 1 are ruminally protected; It is characterized in that; The oil of said deep hydrogenation or fat and the aliphatic acid with the fusing point that is higher than 40 ℃ are selected from down group; Its group comprises hydrogenation rape oil, hydrogenated corn oil, cotmar, hydrogenated groundnut, hydrogenation palm kernel oil, HPO, hydrogenated palm stearic acid, hydrogenation sunflower oil, hydrogenated rapeseed oil, palmitic acid and stearic acid.

9. the method for the choline that the two coatings of preparation according to claim 1 are ruminally protected is characterized in that said choline and said binding agent/coating material are with 1: the weight ratio of 0.1-2.0 is mixed with each other.

10. the method for the choline that the two coatings of preparation according to claim 1 are ruminally protected is characterized in that blender is selected from down group, and its group comprises ribbon mixer, agitator, homogenizer and V-type blender.

11. the method for the choline that the two coatings of preparation according to claim 1 are ruminally protected is characterized in that the extruding machine is an extruder.

12. the method for the choline that the two coatings of preparation according to claim 1 are ruminally protected is characterized in that the choline of encapsulation is graininess or needle-like for the first time.

13. the method according to the ruminally protected choline of the two coatings of claim 1 or 11 described preparations is characterized in that the porous plate in the said extruding machine is of a size of 0.5-2.5mm.

14. the method for the choline that the two coatings of preparation according to claim 11 are ruminally protected is characterized in that the temperature of the recipient in the said extruder remains on 10-60 ℃.

15. the method for the choline that the two coatings of preparation according to claim 1 are ruminally protected; It is characterized in that; Step (d) is carried out through the packaging system second time that is selected from down group, comprises super mixer, ribbon mixer, ROEDIGE blender and fluid bed processor.

16. the method for the choline that the two coatings of preparation according to claim 1 are ruminally protected is characterized in that two coating levels are 15-55% (w/w).