CN118777621B - Filtering anticoagulation blood vessel clot auxiliary device - Google Patents
Filtering anticoagulation blood vessel clot auxiliary device Download PDFInfo
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- CN118777621B CN118777621B CN202411224837.4A CN202411224837A CN118777621B CN 118777621 B CN118777621 B CN 118777621B CN 202411224837 A CN202411224837 A CN 202411224837A CN 118777621 B CN118777621 B CN 118777621B
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Classifications
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/86—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving blood coagulating time or factors, or their receptors
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D61/00—Processes of separation using semi-permeable membranes, e.g. dialysis, osmosis or ultrafiltration; Apparatus, accessories or auxiliary operations specially adapted therefor
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
- B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
- B01L3/5021—Test tubes specially adapted for centrifugation purposes
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/5302—Apparatus specially adapted for immunological test procedures
- G01N33/5304—Reaction vessels, e.g. agglutination plates
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/573—Immunoassay; Biospecific binding assay; Materials therefor for enzymes or isoenzymes
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
- G01N2333/90—Enzymes; Proenzymes
- G01N2333/914—Hydrolases (3)
- G01N2333/948—Hydrolases (3) acting on peptide bonds (3.4)
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Abstract
The invention provides a filtering anticoagulation blood vessel clot auxiliary device which comprises a centrifuge tube, wherein a buoyancy ring is arranged on the inner wall of the centrifuge tube, the buoyancy ring is of a hollow annular structure, the outer edge of the buoyancy ring is connected with the upper part of an elastic ring, the elastic ring is an annular body, the annular body of the buoyancy ring and the annular body of the elastic ring are arranged in a tangent way, a plurality of buoyancy ring holes are arranged on the outer surface of the buoyancy ring, a plurality of elastic ring holes are arranged in an inner cavity of the elastic ring, each buoyancy ring hole is connected with the adjacent elastic ring hole through a traction wire, the traction wire forms a retractive shape through the buoyancy ring holes and the elastic ring holes, the elastic ring is subjected to centripetal elastic deformation under the traction action of the traction wire, the buoyancy ring forms inward and downward pretightening force through the elastic deformation of the elastic ring and the traction action of the traction wire, and the cross section edges of the buoyancy ring and the traction wire are enclosed into a curved triangle.
Description
Technical Field
The invention relates to the technical field of medical equipment, in particular to a filtering anticoagulation blood vessel clot auxiliary device.
Background
Anticoagulation detection is a common blood test, and can be used for evaluating blood coagulation function and detecting related diseases by detecting a plurality of medical indexes including prothrombin time, activated partial thromboplastin time and thrombin time through anticoagulation detection.
In anticoagulation, the prothrombin time, the activated partial thromboplastin time, and the thrombin time index are mostly measured by plasma, so that anticoagulation blood vessels are required, and blood in the anticoagulation blood vessels is centrifuged, and then upper plasma is collected for sampling and measurement. Anticoagulants are generally contained in anticoagulants, and when anticoagulation blood collection is carried out, the blood and the anticoagulants in an anticoagulation tube are required to be fully and uniformly mixed in time, so that the collected blood is in a non-coagulation state.
Because fresh whole blood contains coagulation factors such as platelets, when a hemostix takes blood, the blood is not timely shaken or sufficiently shaken, and other irregular operations are not carried out, so that the collected blood is not timely or sufficiently mixed with an anticoagulant in an anticoagulation vessel, blood clots are generated by blood coagulation, and an anticoagulation detection result is error due to the existence of the blood clots. Macroscopic blood clot inspectors can screen and process in time, but for tiny blood clots, inspectors cannot distinguish in time, and then the workload of inspectors is increased.
Disclosure of Invention
In view of the above problems in the prior art, the present invention provides an auxiliary device for filtering anticoagulated blood vessel clot, which can enable an inspector to screen out anticoagulated blood which has generated a micro clot in time.
The auxiliary device comprises a centrifuge tube, wherein a buoyancy ring is arranged on the inner wall of the centrifuge tube, the buoyancy ring is of a hollow annular structure, the outer edge of the buoyancy ring is connected with the upper part of an elastic ring, the elastic ring is an annular body, the ring body of the buoyancy ring and the ring body of the elastic ring are tangentially arranged, a plurality of buoyancy ring holes are arranged on the outer surface of the buoyancy ring, a plurality of elastic ring holes are arranged in an inner cavity of the elastic ring, each buoyancy ring hole is connected with the adjacent elastic ring hole through a traction wire, the traction wire forms a folded shape through the buoyancy ring holes and the elastic ring holes, the elastic ring is elastically deformed centripetally under the traction action of the traction wire, the buoyancy ring forms inward downward pretightening force through the elastic deformation of the elastic ring and the traction action of the traction wire, the elastic ring, the cross section edges of the buoyancy ring and the traction wire enclose a curved triangle, the cross section edge of the buoyancy ring forms a curved edge, the curved edge is an inner curved edge, the curved edge is a smooth edge, the cross section of the curved edge is a smooth ring, and the cross section of the annular liner is connected with the annular liner, and the cross section of the liner is smooth;
When the traction wire is in a traction and tension state, the elastic ring is subjected to centripetal bending deformation, the upper part of the inner cavity of the elastic ring is clung to the outer surface of the buoyancy ring, the outer diameter of the elastic ring is larger than the inner diameter of the centrifugal tube, and the elastic ring and the centrifugal tube form interference fit;
The outer wall of the smooth lining is attached to the inner wall of the centrifuge tube, the lower end face of the annular body of the smooth lining is provided with a filtering membrane in a connecting mode, the filtering membrane is an elastic membrane, the whole elastic membrane is hemispherical, the elastic membrane is a white visible membrane, and the buoyancy ring, the elastic ring and the smooth lining are made of silica gel;
the traction wire is a medical absorbable fiber wire, the medical absorbable fiber wire contains albumin, a breaking point is arranged in the middle of the traction wire, when the traction wire breaks, the outer surface of the elastic ring is attached to the wall of the centrifugal tube, the elastic ring is in an extending posture, and the outer diameter of the buoyancy ring is smaller than the inner diameter of the centrifugal tube.
The filtering anticoagulation blood vessel clot auxiliary device, wherein the density of the silica gel is 0.3g/cm 3～0.8g/cm3.
Above-mentioned filtering anticoagulation blood vessel clot auxiliary device, wherein, smooth bush adopts the hard silica gel material, smooth bush's surface is smooth surface.
The filtering and anticoagulation blood vessel clot auxiliary device is characterized in that the white visible membrane is a nylon microporous membrane, the nylon microporous membrane is of a porous network structure, and the aperture range of the nylon microporous membrane is 6-12 mu m.
Above-mentioned filtering anticoagulation blood vessel clot auxiliary device, wherein, the centrifuging tube inner wall is equipped with the snap ring, the snap ring with the distance at centrifuging tube top is 1/3~1/2 of centrifuging tube total length.
Above-mentioned filtration anticoagulation blood vessel clot auxiliary device, wherein, the blood volume that the centrifuging tube can hold is 2mL ~5mL.
Above-mentioned filtering anticoagulation blood vessel clot auxiliary device, wherein, buoyancy circle smooth bush reaches the integrated configuration that filtering membrane formed is after the centrifugation by the snap ring is spacing, filtering membrane's the minimum with the distance of centrifuging tube bottom is greater than 1/3 of centrifuging tube total length.
The invention has the beneficial effects that when the anticoagulant liquid is centrifuged, the density of the buoyancy ring, the elastic ring and the smooth lining is lower than that of blood so as to generate buoyancy, meanwhile, as albumin in the medical absorbable albumin wire can be subjected to enzymolysis reaction with enzyme in the blood so as to be absorbed, and meanwhile, as the tension of the traction wire is increased during centrifugation, the traction wire is rapidly broken at a breaking point, so that the buoyancy ring can outwards and upwards overturned, and upwards strengthening force is generated with the buoyancy of the buoyancy ring, the combined structure formed by the buoyancy ring, the elastic ring, the smooth lining and the filtering membrane can antagonize the downward impact force of red blood cells during centrifugation so as to upwards move, and simultaneously, tiny blood clots can be intercepted on the filtering membrane, and a inspector can timely screen and distinguish the anticoagulated blood which has generated tiny clots, and extract plasma samples for examination of prothrombin time, activated partial thrombin time and thrombin time anticoagulation items.
Drawings
FIG. 1 is a front view of a filtered anticoagulant vascular clot assist device in accordance with one embodiment of the present invention prior to anticoagulant addition;
FIG. 2 is an enlarged view of FIG. 1 at A;
FIG. 3 is a front view of a filtered anticoagulant vascular clot assist device according to an embodiment of the present invention after centrifugation;
FIG. 4 is a three-dimensional schematic view of a composite structure formed by buoyancy rings, elastic rings, smooth bushings and filtering membranes;
FIG. 5 is a bottom view of the combined buoyancy ring, elastic ring, smooth bushing and filtering membrane structure;
FIG. 6 is a three-dimensional schematic of a buoyancy ring;
FIG. 7 is an enlarged cross-sectional view of the buoyancy ring, the elastic ring, the buoyancy ring annulus, the elastic ring annulus, and the pull wire.
The reference numerals are 1-centrifuge tube, 2-filtering membrane, 3-clasp, 4-buoyancy ring, 4 a-buoyancy ring annular hole, 5-smooth lining, 6-elastic ring, 6 a-elastic ring annular hole, 7-traction wire, 8-erythrocyte sedimentation layer and 9-plasma layer.
Detailed Description
The invention will be described more fully hereinafter with reference to the accompanying examples in order to facilitate an understanding of the invention, but the invention may be embodied in many different forms and is not limited to the examples described herein. Rather, these embodiments are provided so that this disclosure will be thorough and complete.
Unless defined otherwise, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this invention belongs. The terminology used herein in the description of the invention is for the purpose of describing particular embodiments only and is not intended to be limiting of the invention. The term "and/or" as used herein includes any and all combinations of one or more of the associated listed items.
1-7, A filtering anticoagulation blood vessel clot auxiliary device, including centrifuging tube 1, centrifuging tube 1 inner wall is equipped with buoyancy circle 4, and buoyancy circle 4 is hollow annular structure, and hollow setting lightens buoyancy circle 4 bulk density, further increases buoyancy of buoyancy circle 4, and the outer edge of buoyancy circle 4 links up with the upper portion of elastic ring 6.
In the embodiment, the elastic ring 6 is an annular body, the ring body of the buoyancy ring 4 is tangential to the ring body of the elastic ring 6, a plurality of buoyancy ring holes 4a are formed on the outer surface of the buoyancy ring 4, a plurality of elastic ring holes 6a are formed in the inner cavity of the elastic ring 6, each buoyancy ring hole 4a is connected with the adjacent elastic ring hole 6a through a traction wire 7, the traction wire 7 forms a folded-back shape through the buoyancy ring holes 4a and the elastic ring holes 6a, the elastic ring 6 is subjected to centripetal elastic deformation under the traction action of the traction wire 7, the buoyancy ring 4 forms inward downward pretightening force under the traction action of the elastic ring 6 and the traction wire, the cross section edges of the elastic ring 6, the buoyancy ring 4 and the traction wire 7 are enclosed into a curved triangle, the curved edge of the curved triangle is an inward concave curved edge, the lower part of the elastic ring 6 is connected with the upper part of the smooth lining 5, the smooth lining 5 is an annular body, and the outer edge of the annular cross section of the smooth lining 5 is in an arc profile.
In this embodiment, when the traction wire 7 is in a traction and tension state, the elastic ring 6 is subjected to centripetal bending deformation, the upper part of the inner cavity of the elastic ring 6 is clung to the outer surface of the buoyancy ring 4, and pressure is generated on the elastic ring 6, so that the outer diameter of the elastic ring 6 is larger than the inner diameter of the centrifuge tube 1, the elastic ring 6 and the centrifuge tube 1 form interference fit, the ring body of the elastic ring 6 can be tightly attached to the inner wall of the centrifuge tube 1, and the combined structure formed by the buoyancy ring 4, the elastic ring 6, the smooth lining 5 and the filtering membrane 2 is placed at the bottom of the centrifuge tube 1.
The outer wall of the smooth lining 5 is attached to the inner wall of the centrifuge tube 1, the lower end face of the annular body of the smooth lining 5 is connected with the filtering membrane 2, the filtering membrane 2 is an elastic membrane, the whole of the elastic membrane is hemispherical, and the hemispherical design and elastic arrangement of the filtering membrane 2 can buffer the impact force of erythrocytes under the action of centrifugal force, so that the damage of erythrocyte membranes is avoided.
In this embodiment, the elastic membrane is a white visual membrane, so that the inspector can observe blood clots trapped on the white visual membrane, and the buoyancy ring 4, the elastic ring 6 and the smooth lining 5 are made of silica gel.
In this embodiment, the traction wire 7 is a medical absorbable fiber wire, and the medical absorbable fiber wire contains albumin, and the albumin in the medical absorbable albumin wire can be subjected to enzymolysis reaction with enzymes in blood so as to be absorbed.
In this embodiment, a breaking point is provided in the middle of the traction wire 7, and the traction wire 7 breaks due to the enzymolysis of albumin and the increase of tension applied during centrifugation. When the traction wire 7 breaks, the elastic ring 6 is in an extending posture, the outer surface of the elastic ring 6 is attached to the wall of the centrifuge tube 1, blood is prevented from being left in a gap between the inner wall of the centrifuge tube 1 and the outer surface of the elastic ring 6, the outer diameter of the buoyancy ring 4 is smaller than the inner diameter of the centrifuge tube 1, and accordingly floating resistance of the buoyancy ring 4 is reduced.
Through above-mentioned structure setting for this device is when carrying out centrifugation to anticoagulation liquid, through buoyancy circle 4, elastic ring 6, smooth bush 5's density is less than the density of blood and then the buoyancy that produces, traction wire 7 fracture, elastic ring 6 is released and is the extension gesture, make buoyancy circle 4 produce outwards ascending upset, and produce ascending stiffening force with buoyancy of buoyancy circle 4, make buoyancy circle 4, elastic ring 6, smooth bush 5 and filter membrane 2 the integrated configuration that forms can antagonize the decurrent impact force of red blood cell when centrifuging, and then upward movement, simultaneously, tiny blood clot can be intercepted on visual membrane, the inspection personnel can in time screen and distinguish the anticoagulation blood that has produced tiny clot.
Furthermore, in the present embodiment, the buoyancy ring 4, the elastic ring 6 and the smooth bushing 5 are made of silica gel with a density of 0.3g/cm 3～0.8g/cm3, so that the buoyancy ring 4 can generate a large buoyancy in blood.
In this embodiment, the smooth bushing 5 is made of hard silica gel. The outer surface of the smooth lining 5 is a smooth surface, so that the smooth lining 5 before centrifugation can be tightly attached to the inner wall of the centrifuge tube 1, the sliding friction force born by the outer surface of the smooth lining 5 is reduced during centrifugation, and further the rising resistance of the smooth lining 5 is reduced, and the inner surface of the smooth lining 5 is a smooth surface, so that erythrocytes or blood clots slide onto the filter membrane 2 along the inner wall of the smooth lining 5 during centrifugation.
In this embodiment, the filtering membrane 2 is preferably a nylon micro-pore filtering membrane, the nylon micro-pore filtering membrane is in a porous network structure, the pore diameter of the nylon micro-pore filtering membrane 2 ranges from 6 μm to 12 μm, and the size of a single red blood cell can smoothly pass through the single red blood cell to intercept micro blood clots, meanwhile, due to the elastic arrangement of the nylon micro-pore filtering membrane 2, the extrusion force of the red blood cell when passing through the filtering membrane 2 can be reduced, so that the damage of the red blood cell membrane is caused.
In this embodiment, the centrifuging tube 1 inner wall is equipped with snap ring 3, the distance of snap ring 3 and centrifuging tube 1 top is 1/3~1/2 of centrifuging tube 1 total length, the blood volume that centrifuging tube 1 can hold is 2mL ~5mL, the integrated configuration that buoyancy ring 4, smooth bush 5 and filtration membrane 2 formed is spacing by snap ring 3 after centrifuging, the minimum of filtration membrane 2 is greater than 1/3 of centrifuging tube 1 total length with the distance of centrifuging tube 1 bottom, the height of red blood cell sedimentation layer 8 after centrifuging is less than 1/3 of centrifuging tube 1 total length, consequently, in the anticoagulation after centrifuging, filtration membrane 2 can stay in the lower floor of plasma layer 9, be convenient for inspection personnel observe whether the nylon has intercepted little blood clot on dragon microporous filtration membrane 2.
The working procedure of this embodiment is as follows:
1. the blood sampling personnel adopts the anticoagulation vessel to collect blood of a patient, and timely shakes and mixes the anticoagulation vessel uniformly, so that the blood sample and the anticoagulation agent are fully mixed to obtain the anticoagulation sample.
2. For samples suspected of having a small blood clot, the present device may be used for clot detection. The whole body composed of the buoyancy ring 4, the elastic ring 6, the smooth lining 5 and the filtering membrane 2 which are pulled by the pulling wire 7 is placed at the bottom of the centrifuge tube 1 by a detector, the clamping ring 3 is clamped at 1/3-1/2 of the inner wall of the centrifuge tube 1, an anticoagulated blood sample which is required to be subjected to blood clot detection is poured into the centrifuge tube 1 of the device, the tube cover is tightly covered, the tube cover is placed into the centrifuge, and the parameter is set to 3000r/min for 15 minutes. Through buoyancy circle 4, elastic ring 6, smooth bush 5's density is less than the buoyancy that the density produced of blood, simultaneously, owing to the fracture of traction wire 7, the decurrent traction force of centripetal of elastic ring 6 and buoyancy circle 4 disappears, elastic ring 6 is released and is the extension gesture, drive buoyancy circle 4 simultaneously and take place outwards ascending upset for the integrated configuration that buoyancy circle 4, elastic ring 6, smooth bush 5 and filtration membrane 2 formed can antagonize the downward impact force of red blood cell when centrifuging, and then upward movement, simultaneously, tiny blood clot can be held back on filtration membrane 2.
3. After centrifugation, according to the condition of the filtering anticoagulation blood clot auxiliary device, screening and distinguishing whether blood clots are generated in anticoagulation or not, wherein the three conditions are as follows:
(1) When the buoyancy ring 4 is limited by the clasp ring 3, the combined structure formed by the buoyancy ring 4, the elastic ring 6, the smooth lining 5 and the filtering membrane 2 is positioned at the lower part of the plasma layer 9, and no blood clot exists on the filtering membrane 2, so that anticoagulation is effective anticoagulation, and anticoagulation detection can be further carried out by taking the plasma on the upper layer of the plasma.
(2) When the buoyancy ring 4 is limited by the clasp 3 or is not limited by the clasp 3, the combined structure formed by the buoyancy ring 4, the elastic ring 6, the smooth lining 5 and the filtering membrane 2 is positioned at the lower part of the plasma layer 9, and blood clots are trapped on the filtering membrane 2, so that anticoagulation is ineffective anticoagulation.
(3) When the buoyancy ring 4 is not limited by the clasp 3, the filtering membrane 2 is not lifted to the plasma layer 9, and it can be judged that anticoagulation blood is ineffective, the buoyancy ring 4 is clamped by the sterile clamp holder, the combined structure formed by the buoyancy ring 4, the elastic ring 6, the smooth lining 5 and the filtering membrane 2 is lifted to the plasma layer 9, and further anticoagulation is confirmed to be ineffective anticoagulation.
The foregoing examples illustrate only a few embodiments of the invention and are described in detail herein without thereby limiting the scope of the invention. It should be noted that it will be apparent to those skilled in the art that several variations and modifications can be made without departing from the spirit of the invention, which are all within the scope of the invention. Accordingly, the scope of protection of the present invention is to be determined by the appended claims.
Claims (4)
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| CN202411224837.4A CN118777621B (en) | 2024-09-03 | 2024-09-03 | Filtering anticoagulation blood vessel clot auxiliary device |
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| CN202411224837.4A CN118777621B (en) | 2024-09-03 | 2024-09-03 | Filtering anticoagulation blood vessel clot auxiliary device |
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| CN118777621B true CN118777621B (en) | 2025-03-04 |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN221208137U (en) * | 2023-11-30 | 2024-06-25 | 天津市肿瘤医院（天津医科大学肿瘤医院） | Detection device convenient for observing anticoagulation micro clot |
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| US20030205538A1 (en) * | 2002-05-03 | 2003-11-06 | Randel Dorian | Methods and apparatus for isolating platelets from blood |
| CN106582908B (en) * | 2016-11-30 | 2019-09-13 | 深圳市达科为生物工程有限公司 | A kind of platelet rich plasma fast separation device and method |
| CN206444635U (en) * | 2016-12-28 | 2017-08-29 | 上海源培生物科技股份有限公司 | Point liquid type centrifuge tube provided with buoyancy post |
| DE102019121723A1 (en) * | 2019-08-13 | 2021-02-18 | Sarstedt Ag & Co. Kg | Separation bodies and methods for separating blood plasma and blood cells |
| MX2022004999A (en) * | 2019-10-31 | 2022-07-27 | Eclipse Medcorp Llc | Systems, methods and apparatus for separating components of a sample. |
| CN112932483A (en) * | 2021-02-02 | 2021-06-11 | 西安交通大学医学院第一附属医院 | Sodium citrate anticoagulation tube with function of detecting micro-clots |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN221208137U (en) * | 2023-11-30 | 2024-06-25 | 天津市肿瘤医院（天津医科大学肿瘤医院） | Detection device convenient for observing anticoagulation micro clot |
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