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CN118393164A - Sample analyzer and control method thereof - Google Patents

Sample analyzer and control method thereof Download PDF

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Publication number
CN118393164A
CN118393164A CN202410377214.4A CN202410377214A CN118393164A CN 118393164 A CN118393164 A CN 118393164A CN 202410377214 A CN202410377214 A CN 202410377214A CN 118393164 A CN118393164 A CN 118393164A
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CN
China
Prior art keywords
sample
bubble sensor
sampling needle
detection
amount
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Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
CN202410377214.4A
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Chinese (zh)
Inventor
王超
舒一馨
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Maccura Medical Electronics Co Ltd
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Maccura Medical Electronics Co Ltd
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Publication date
Application filed by Maccura Medical Electronics Co Ltd filed Critical Maccura Medical Electronics Co Ltd
Priority to CN202410377214.4A priority Critical patent/CN118393164A/en
Publication of CN118393164A publication Critical patent/CN118393164A/en
Pending legal-status Critical Current

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    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
    • G01N35/10—Devices for transferring samples or any liquids to, in, or from, the analysis apparatus, e.g. suction devices, injection devices
    • G01N35/1009—Characterised by arrangements for controlling the aspiration or dispense of liquids
    • G01N35/1016—Control of the volume dispensed or introduced
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00—Sampling; Preparing specimens for investigation
    • G01N1/02—Devices for withdrawing samples
    • G01N1/10—Devices for withdrawing samples in the liquid or fluent state
    • G01N1/14—Suction devices, e.g. pumps; Ejector devices
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
    • G01N21/25—Colour; Spectral properties, i.e. comparison of effect of material on the light at two or more different wavelengths or wavelength bands
    • G01N21/31—Investigating relative effect of material at wavelengths characteristic of specific elements or molecules, e.g. atomic absorption spectrometry
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
    • G01N21/47—Scattering, i.e. diffuse reflection
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N29/00—Investigating or analysing materials by the use of ultrasonic, sonic or infrasonic waves; Visualisation of the interior of objects by transmitting ultrasonic or sonic waves through the object
    • G01N29/02—Analysing fluids
    • G01N29/028—Analysing fluids by measuring mechanical or acoustic impedance
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
    • G01N35/00584—Control arrangements for automatic analysers
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
    • G01N35/10—Devices for transferring samples or any liquids to, in, or from, the analysis apparatus, e.g. suction devices, injection devices
    • G01N35/1002—Reagent dispensers

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  • Physics & Mathematics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Biochemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Analytical Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Pathology (AREA)
  • Spectroscopy & Molecular Physics (AREA)
  • Acoustics & Sound (AREA)
  • Hydrology & Water Resources (AREA)
  • Automatic Analysis And Handling Materials Therefor (AREA)

Abstract

The embodiment of the invention provides a sample analyzer and a control method thereof, wherein the sample analyzer comprises a sampling needle, a liquid path pipe and a driver which are sequentially connected, and a first bubble sensor is arranged at a preset position on the liquid path pipe; the processor is used for: the control driver drives the sampling needle to absorb the sample from the sample carrying container, wherein the absorption amount is the sum of the detection dosage and the auxiliary judgment amount; after the sample suction is finished, if a sample exists on the section where the first bubble sensor is located, judging that the suction detection dosage is sufficient; if no sample exists on the section where the first bubble sensor is located, the insufficient detection dosage of the suction is judged. After the sample suction is finished, whether the sucked sample quantity for detection is sufficient or not is judged through a bubble sensor arranged on the liquid path pipe, so that the cost is low, erroneous judgment can be effectively avoided, and the judgment accuracy is improved.

Description

Sample analyzer and control method thereof
Technical Field
The embodiment of the invention relates to the technical field of medical equipment, in particular to a sample analyzer and a control method thereof.
Background
Sample analyzers are high-sensitivity, high-specificity analyzers for analyzing a specific sample and obtaining a corresponding analysis result, and find wide application in clinical tests, such as testing various indexes of blood, urine or other body fluid samples. The workflow of sample analysis is: firstly, conveying a sample tube to the lower part of a sampling needle, controlling a moving mechanism to drive the sampling needle to descend from an original point position by a fixed height, enabling the sampling needle to extend into the sample tube to suck samples, and then supplying the sucked samples to a detection module for detection; or the sucked sample is supplied to a reaction container, and the sample is mixed with a reagent in the reaction container to form a reaction liquid for detection. The sample sucked by the sampling needle is insufficient due to the difference of the sample amounts in different sample tubes or the possible movement errors of the moving mechanism. Whether the sample sucked by the sampling needle is sufficient or not directly influences the supply quantity of the subsequent sample and influences the accuracy of the detection result.
At present, a liquid level detection method is mainly adopted for judging the sample size, the liquid level detection method which is the mainstream in the industry at present is a capacitance detection method, and for example, a patent with the publication number of CN204027651U and the name of test tube sample size detection device is judged by adopting the capacitance detection method. Specifically, the detection device comprises a liquid level sensing circuit board, the liquid level sensing circuit board comprises a capacitance sensing driving circuit, an analog signal processing circuit and a level signal circuit which are sequentially connected, a sampling needle is made of conductive materials, the upper portion of the sampling needle is fixed on a sampling needle fixing frame in an insulating mode, the sampling needle is electrically connected with the capacitance sensing driving circuit of the liquid level sensing circuit board through a conducting wire, and a signal of the level signal circuit is connected to a main controller to realize liquid level detection. However, the device needs to perform insulation treatment on the sampling needle, and a special liquid level sensing circuit board is adopted, so that the cost is high, and misjudgment can be caused by the conditions that liquid films exist on the liquid level, bubbles exist on the liquid films, the sampling needle contacts the container wall, small liquid drops exist on the container wall above the liquid level, and the like.
Disclosure of Invention
The embodiment of the invention provides a sample analyzer and a control method thereof, which can judge whether the amount of a sample for detection sucked after the sample suction is finished is sufficient or not, and avoid the influence of the insufficient amount of the sample for detection sucked on the detection of a subsequent sample.
In a first aspect, an embodiment of the present invention provides a sample analyzer, including: the device comprises a sampling needle, a liquid path pipe, a first bubble sensor, a driver and a processor;
the sampling needle, the liquid path pipe and the driver are sequentially connected, and the first bubble sensor is arranged at a preset position on the liquid path pipe;
The processor is used for:
the control driver drives the sampling needle to absorb the sample from the sample bearing container, wherein the absorption amount is the sum of the detection dosage and the auxiliary judgment amount, and the auxiliary judgment amount is determined according to the content volume of the sampling needle and the position of the first bubble sensor;
after the sample suction is finished, judging whether a sample exists on the section where the first bubble sensor is positioned according to the electric signal output by the first bubble sensor;
If the section where the first bubble sensor is located is provided with a sample, judging that the sucked detection dosage is sufficient;
if no sample exists on the section where the first bubble sensor is located, the insufficient detection dosage of the suction is judged.
In one embodiment, the processor is further configured to:
if the detected amount of the suction is determined to be sufficient, the sampling needle is controlled to discharge the sample into the sample-carrying container before the supply of the sample to the detection module is controlled, and the discharge amount is an auxiliary determination amount.
In one embodiment, the liquid path tube further comprises a second bubble sensor, wherein the second bubble sensor is arranged on the liquid path tube, and the distance between the second bubble sensor and the sampling needle is smaller than the distance between the first bubble sensor and the sampling needle.
In one embodiment, the processor is further configured to:
And when the section where the first bubble sensor is positioned is provided with a sample or the section where the second bubble sensor is positioned is provided with a sample, judging that the sucked detection dosage is sufficient.
In one embodiment, the processor is further configured to determine the auxiliary decision amount as follows:
And controlling the driver to drive the sampling needle to suck air, continuously monitoring whether a sample exists on the section where the first bubble sensor is positioned according to the electric signal output by the first bubble sensor in the process, stopping sucking the sample when no sample exists on the section is monitored, and determining the sucking amount at the moment as the auxiliary judgment amount.
In a second aspect, an embodiment of the present invention provides a method for controlling a sample analyzer, including:
The control driver drives the sampling needle to absorb the sample from the sample carrying container, wherein the absorption amount is the sum of the detection dosage and the auxiliary judgment amount;
after the sample suction is finished, judging whether a sample exists on the section where the first bubble sensor is positioned according to an electric signal output by the first bubble sensor, wherein the first bubble sensor is arranged at a preset position on a liquid path pipe connected with a sampling needle;
If the section where the first bubble sensor is located is provided with a sample, judging that the sucked detection dosage is sufficient;
if no sample exists on the section where the first bubble sensor is located, the insufficient detection dosage of the suction is judged.
In one embodiment, if the detected amount of suction is determined to be sufficient, the method further comprises:
Before the sample is supplied to the detection module, the sampling needle is controlled to discharge the sample into the sample carrying container, and the discharge amount is an auxiliary judgment amount.
In one embodiment, the method further comprises:
when a sample exists on the section where at least one of the first bubble sensor and the second bubble sensor is located, the sufficient detection dosage is judged to be absorbed, the second bubble sensor is arranged on the liquid path pipe, and the distance between the second bubble sensor and the sampling needle is smaller than the distance between the first bubble sensor and the sampling needle.
In one embodiment, the method further comprises determining the auxiliary decision amount prior to performing the sample detection as follows:
And controlling the driver to drive the sampling needle to suck air, continuously monitoring whether a sample exists on the section where the first bubble sensor is positioned according to an electric signal output by the first bubble sensor in the process, stopping sucking the sample when no sample exists on the section is monitored, and determining the sucking amount at the moment as an auxiliary judgment amount.
In a third aspect, embodiments of the present invention provide a computer-readable storage medium having stored therein computer-executable instructions which, when executed by a processor, are adapted to carry out a method of controlling a sample analyzer according to any of the second aspects.
The embodiment of the invention provides a sample analyzer and a control method thereof, wherein the sample analyzer comprises: the device comprises a sampling needle, a liquid path pipe, a first bubble sensor, a driver and a processor; the sampling needle, the liquid path pipe and the driver are sequentially connected, and the first bubble sensor is arranged at a preset position on the liquid path pipe; the processor is used for: the control driver drives the sampling needle to absorb the sample from the sample bearing container, wherein the absorption amount is the sum of the detection dosage and the auxiliary judgment amount, and the auxiliary judgment amount is determined according to the content volume of the sampling needle and the position of the first bubble sensor; after the sample suction is finished, judging whether a sample exists on the section where the first bubble sensor is positioned according to the electric signal output by the first bubble sensor; if the section where the first bubble sensor is located is provided with a sample, judging that the sucked detection dosage is sufficient; if no sample exists on the section where the first bubble sensor is located, the insufficient detection dosage of the suction is judged. After the sample is sucked, whether the detected consumption of suction is sufficient or not is judged through the bubble sensor arranged on the liquid path pipe, so that the cost is low, and misjudgment caused by the conditions that liquid films exist on the liquid surface, bubbles exist on the liquid films, sampling needles contact the container wall, small liquid drops exist on the container wall above the liquid surface and the like can be effectively avoided, so that the judgment accuracy is improved, and further the accuracy of sample analysis results is improved.
Drawings
The accompanying drawings, which are incorporated in and constitute a part of this specification, illustrate embodiments consistent with the invention and together with the description, serve to explain the principles of the invention.
FIG. 1 is a schematic diagram of a portion of a sample analyzer according to an embodiment of the present invention;
FIG. 2 is a schematic view of a portion of a sample analyzer according to another embodiment of the present invention;
FIG. 3 is a flowchart of a control method of a sample analyzer according to an embodiment of the present invention;
fig. 4 is a flowchart of a control method of a sample analyzer according to another embodiment of the present invention.
Specific embodiments of the present invention have been shown by way of the above drawings and will be described in more detail below. The drawings and the written description are not intended to limit the scope of the inventive concepts in any way, but rather to illustrate the inventive concepts to those skilled in the art by reference to the specific embodiments.
Detailed Description
The application will be described in further detail below with reference to the drawings by means of specific embodiments. Wherein like elements in different embodiments are numbered alike in association. In the following embodiments, numerous specific details are set forth in order to provide a better understanding of the present application. However, one skilled in the art will readily recognize that some of the features may be omitted, or replaced by other elements, materials, or methods in different situations. In some instances, related operations of the present application have not been shown or described in the specification in order to avoid obscuring the core portions of the present application, and may be unnecessary to persons skilled in the art from a detailed description of the related operations, which may be presented in the description and general knowledge of one skilled in the art.
Furthermore, the described features, operations, or characteristics of the description may be combined in any suitable manner in various embodiments. Also, various steps or acts in the method descriptions may be interchanged or modified in a manner apparent to those of ordinary skill in the art. Thus, the various orders in the description and drawings are for clarity of description of only certain embodiments, and are not meant to be required orders unless otherwise indicated.
The numbering of the components itself, e.g. "first", "second", etc., is used herein merely to distinguish between the described objects and does not have any sequential or technical meaning. The term "coupled" as used herein includes both direct and indirect coupling (coupling), unless otherwise indicated.
In the prior art, the motor operation pulse of the driver is controlled to control the sucked detection dosage, but the sucked detection dosage is the detection dosage which is expected to be sucked and is not the actual sucked detection dosage of the sampling needle. Due to the fact that the sample amounts in different sample tubes are different or the movement errors and the like of the moving mechanism possibly exist, the detection dosage actually sucked by the sampling needle is insufficient. For the existing liquid level detection method, a large number of abnormal conditions may exist, for example, the liquid level of a sample carrying container (such as a test tube) has a liquid film, bubbles exist, a sampling needle contacts the container wall, and small liquid drops exist on the container wall above the liquid level, so that the liquid level detection is misjudged, and the subsequent detection dosage actually sucked does not reach the detection dosage required to be sucked. In addition, for the existing liquid level detection method, even if the liquid level height can be accurately detected to judge that the sample amount in the sample tube meets the requirement, if the moving mechanism for driving the sample needle to move in the subsequent sampling process has a movement error and other reasons, the sample needle does not drop to the preset height, the insufficient detection dosage can be caused.
The effect of insufficient detection volume of aspiration will be described below using a urine analyzer as an example. Urine analyzers include three detection principles: the three detection principles of urine tangible detection, physical detection and urine dry chemical detection can be integrated on one instrument, and can be independently or combined on different urine analysis instruments.
If the amount of the sample for detection sucked by the urine analyzer is sufficient, once misjudgment is carried out, the insufficient urine component detection sample can be caused to influence the accuracy of the urine tangible detection result, or the insufficient physical detection sample can cause the inaccuracy of the physical detection result, or the successful detection can be influenced because the sample can not be normally dropped on the dry chemical test strip in the dry chemical detection.
Fig. 1 is a schematic diagram of a portion of a sample analyzer according to an embodiment of the invention. As shown in fig. 1, the sample analyzer provided in this embodiment may include: a sampling needle 11, a liquid path tube 12, a first bubble sensor 13, a driver 15 and a processor 16; as shown, the sampling needle 11, the liquid path tube 12, and the driver 15 are sequentially connected, and the first bubble sensor 13 is disposed at a preset position on the liquid path tube 12. Wherein the sampling needle 11 is used for transferring samples and/or reagents. It will be appreciated that the sample analyzer also includes a needle movement mechanism (not shown) for supporting the sampling needle and driving the sampling needle in horizontal and vertical movement. The processor 16 is electrically connected to the first bubble sensor 13 and the driver 15, respectively, for: the control driver drives the sampling needle to absorb the sample from the sample bearing container, wherein the absorption amount is the sum of the detection dosage and the auxiliary judgment amount, and the auxiliary judgment amount is determined according to the content of the sampling needle and the position of the first bubble sensor; after the sample suction is finished, judging whether a sample exists on the section where the first bubble sensor is positioned according to the electric signal output by the first bubble sensor; if the section where the first bubble sensor is located is provided with a sample, judging that the sucked detection dosage is sufficient; if no sample exists on the section where the first bubble sensor is located, the insufficient detection dosage of the suction is judged.
The sample needle stretches into the sample bearing container to suck the sample below the sample liquid level, the sample liquid level gradually descends along with the sample suction, and after the sample suction is completed, if the sample suction opening of the sample needle is still positioned below the liquid level (including the liquid level), the detection dosage sucked by the sample needle is enough, and the sample suction opening of the sample needle is full of the sample; if the sample sucking port of the sample needle is positioned above the liquid level, the suction condition exists in the second half section of the sample sucking, the detection dosage of the sample needle sucking is insufficient, and an air section exists at the sample sucking port of the sample needle. The invention fixedly sets a first bubble sensor on a liquid path pipe connected with a sampling needle, and controls a driver to drive the sampling needle to suck a sample from a sample bearing container; the suction quantity is the sum of the detection quantity and the auxiliary judgment quantity, and the auxiliary judgment quantity is determined according to the content of the sampling needle and the position of the first bubble sensor; after the sample suction is finished, judging whether a sample exists on the section where the first bubble sensor is positioned according to the electric signal output by the first bubble sensor, so as to judge whether the sample exists at the sample suction port of the sampling needle after the sampling needle sucks the sample amount, and further judge whether the sucked detection amount is sufficient.
Compared with the prior art that whether the sample amount in the sample bearing container is sufficient or not before the sample is sucked, the method can effectively avoid misjudgment caused by the conditions that the liquid level is provided with a liquid film, the liquid film is provided with bubbles, the sampling needle is contacted with the container wall, the container wall above the liquid level is provided with small liquid drops and the like. In addition, the application can identify the condition of insufficient sample suction quantity caused by the motion error of the moving mechanism, avoid the influence on the subsequent detection caused by the insufficient sample suction quantity of the sampling needle, and is beneficial to improving the accuracy of the sample analysis result. Meanwhile, the application does not need to carry out insulation treatment on the sampling needle and adopt a special liquid level sensing circuit board, and the sampling needle is not required to be electrically connected with the liquid level sensing circuit board through a lead, so that the detection cost is low.
The detection amount in the present embodiment may be determined by the type of sample detection, for example, a correspondence relationship between the detection type and the detection amount may be established in advance; the auxiliary determination is determined by the content of the sampling needle and the position of the first bubble sensor.
Further, the sample analyzer in this embodiment further includes a detection module, which may be a dry urine chemical detection module, a tangible urine detection module, or a physical urine detection module. If the amount of the sucked test solution is determined to be sufficient, the test module may be supplied with the sample, for example, a dry test strip in the dry chemical test module may be dropped with the sample, or the sample may be supplied to the urine tangible test module or the physical test module through a pipeline. And if the detected amount of the suction is insufficient, alarming. On the basis of the above embodiments, the processor in the sample analyzer provided in this embodiment is further configured to: the multi-sucked sample is recovered before the sample is supplied to the detection module, specifically, the sample is discharged from the sampling needle to the sample carrying container, and the discharge amount is an auxiliary judgment amount. Through spit out auxiliary judgement volume's sample to the sample in carrying the container, only leave the sample that detects the quantity and be used for carrying out sample analysis, can avoid sampling needle front end not to have the sample and exist the air and lead to dropping the condition that exists the drip to the test strip in the dry chemical detection module of urine when dripping the sample, can guarantee again that the sample detects the quantity, avoided the waste of sample. This is because in the present application, in order to detect whether or not the amount of detection sucked is sufficient, the amount of sampling needle sucked is the sum of the amount of detection and the auxiliary determination amount. If the section of the first bubble sensor is provided with a sample, the sufficient detection dosage is judged, namely the sampling needle sample sucking port does not have air after the detection dosage is absorbed, but the sampling needle sample sucking port does not have air after the detection dosage is absorbed+the auxiliary judgment quantity. If the sampling needle sucks the sample, and air exists, the situation that the sample is leaked when the sample is dropped on the test paper strip in the urine dry chemical detection module can be caused. The auxiliary judgment amount is discharged before the sample is supplied to the detection module, so that the situation of missing drop can be effectively avoided, the normal operation of drop samples is ensured, the accuracy of drop sample results is ensured, the sample detection dosage is ensured, and the waste of the sample is avoided.
If bubbles adhere to the wall of the liquid path in the sample sucking process, the first bubble sensor may be misjudged, and in order to further improve the judgment accuracy, in this embodiment, a compact installation manner of two bubble sensors is adopted, so as to avoid misjudgment of the first bubble sensor caused by the above situation. Fig. 2 is a schematic view of a portion of a sample analyzer according to another embodiment of the present invention. As shown in fig. 2, the sample analyzer provided in this embodiment further includes a second bubble sensor 17 based on the embodiment shown in fig. 1, where the second bubble sensor 17 is disposed on the liquid path tube 12, and a distance between the second bubble sensor 17 and the sampling needle 11 is smaller than a distance between the first bubble sensor 13 and the sampling needle 11, that is, the second bubble sensor is disposed below the first bubble sensor, or the second bubble sensor is disposed between the sampling needle and the first bubble sensor. Wherein the distance between the second bubble sensor 17 and the first bubble sensor 13 can be as small as possible on the basis of meeting the requirements of the bubble sensor installation space to ensure that both bubble sensors are compactly installed.
In the sample analyzer using two bubble sensors mounted in a compact manner, if one of the two bubble sensors is judged to be liquid, it can be judged that the amount of detection sucked is sufficient, and the sample can be normally supplied to the detection module. That is, the processor for sample analysis in this embodiment is also configured to: and when the section where the first bubble sensor is positioned is provided with a sample or the section where the second bubble sensor is positioned is provided with a sample, judging that the detection sample amount is sufficient.
The sample analyzer provided by the embodiment is further based on the embodiment, and by adopting two bubble sensors, the false judgment of the bubble sensors caused by the fact that individual bubbles adhere to the wall of the liquid path in the sample sucking process is effectively avoided, and the judgment accuracy is further improved.
The bubble sensor in this embodiment may be a photoelectric bubble sensor or an ultrasonic bubble sensor. When the bubble sensor is a photoelectric bubble sensor, the bubble sensor comprises a light emitter and a light receiver which are fixedly arranged on two sides of a liquid path pipe, the liquid path pipe adopts a light-transmitting liquid path pipe, the relation between output voltage and illumination intensity can be obtained according to the volt-ampere characteristic of the photoelectric effect of the bubble sensor through the photoelectric effect of the photoelectric device of the bubble sensor, and when bubbles exist in the light-transmitting liquid path pipe, the light receiver can receive light intensity to change due to the fact that light reflection and absorption of different mediums on light are different, so that the change of the output voltage is caused. The processor can distinguish different media in the liquid path pipe according to the electric signals output by the bubble sensor, so as to judge whether the section where the bubble sensor is positioned has air or a sample. When the bubble sensor is an ultrasonic bubble sensor, the bubble sensor comprises an ultrasonic emitter and an ultrasonic receiver which are fixedly arranged on two sides of the liquid path pipe, and the light transmittance of the liquid path pipe is not limited. The ultrasonic bubble sensor utilizes the principle that acoustic impedances of ultrasonic waves in liquid and gas are different to realize identification and detection of bubbles or liquid. At this time, ultrasonic waves are emitted and received by the bubble sensor, and the acoustic impedance is different in different media, so that the output electric signal is changed. When the bubble exists, the ultrasonic wave can be reflected back and can not reach the receiving end due to the large change of the impedance of the sound wave, so that the bubble exists on the section where the bubble sensor is located. The bubble sensor in this embodiment is not limited in type as long as the bubble sensor can be used for identifying and detecting bubbles or liquid in a certain section of the liquid path pipe. In order to reduce the cost, it is preferable that the first bubble sensor and the second bubble sensor are photoelectric bubble sensors.
On the basis of any of the above embodiments, how the auxiliary determination amount is determined will be further described below. The auxiliary determination amount X is determined by the content volume of the sampling needle and the mounting position of the first bubble sensor. The first bubble sensor is arranged at a preset position on the liquid path pipe. If the internal volume of the sampling needle 11 is a, and the internal volume of the pipeline between the preset position on the liquid pipeline 12 and the sampling needle is b, the auxiliary determination quantity X is more than or equal to a+b. Preferably, the auxiliary determination amount X > a+b. The driver should particularly emphasize that the auxiliary determination amount in this embodiment is preferably larger than (a+b) in order to avoid the influence of bubbles generated during the critical level sample suction on the sample detection result. The critical level refers to a level of liquid in the sample-carrying container below the level of the sample. If the sampling needle sample suction port descends to be above the critical liquid level, insufficient detection dosage is caused; if the sample suction port just descends to the critical liquid level, the detection dosage sucked in theory is just enough; if the sample sucking port is lowered below the critical liquid level, the sucked detection dosage is enough. Although the sample sucking port just descends to the critical liquid level, the detection amount sucked in theory is just enough, namely the sample sucking port of the sampling needle is filled with samples; however, in actual operation, bubbles may exist at the sample suction port of the sampling needle after the detection amount is sucked due to the reasons of liquid level fluctuation, liquid level tension and the like. Under the condition, the detection is carried out, the sufficient detection dosage can be possibly identified, but samples are further supplied to the detection module, for example, the dry chemical test paper in the urine dry chemical detection module drops, so that the condition that the sampling needle hangs liquid and the dropping amount is inaccurate or the dropping fails can be caused, and the normal operation of the detection or the accuracy of the detection result is affected. When the sample sucking port of the sampling needle descends to the critical liquid level for sucking samples, the liquid section mixed with bubbles at the end of the sample sucking port can pass through the first bubble sensor and move into a pipeline behind the first bubble sensor, so that the detection result is insufficient in the sucked detection dosage, and the influence of the bubbles generated when the sampling needle descends to the critical liquid level of the sample liquid level in the sample bearing container for sucking samples on the detection result of the sample is avoided. The larger the auxiliary judgment amount is, the influence of bubbles generated during critical liquid level sample suction on subsequent detection can be effectively avoided, but the longer the driver sample suction time is caused by the excessive auxiliary judgment amount, so the auxiliary judgment amount can be properly increased under the condition of time permission. The conventional recommended amount X is not less than 100. Mu.l, preferably X is not less than 100. Mu.l.
In an alternative implementation, the processor of the sample analyzer provided in this embodiment may be further configured to determine the auxiliary determination amount before performing the sample detection according to the following steps: and controlling the driver to drive the sampling needle to suck the special reagent from the reagent container, continuously monitoring whether the special reagent exists on the section where the first bubble sensor is positioned according to the electric signal output by the first bubble sensor in the process, stopping sucking the reagent when the special reagent exists on the section is monitored, and determining the sucking quantity at the moment as the auxiliary judgment quantity. It should be noted that, because the sampling needle is filled with the common cleaning liquid before the sample is sucked, both the sampling needle and the liquid path are filled with the common cleaning liquid. Therefore, in order to accurately determine the auxiliary determination amount, it is necessary to use a specific reagent different from the ordinary cleaning liquid. In order to further reduce the cost, in another alternative implementation, the processor of the sample analyzer provided in this embodiment may be further configured to determine the auxiliary determination amount before performing the sample detection according to the following steps: and controlling the driver to drive the sampling needle to suck air, continuously monitoring whether a sample exists on the section where the first bubble sensor is positioned according to the electric signal output by the first bubble sensor in the process, stopping sucking the sample when no sample exists on the section is monitored, and determining the sucking amount at the moment as the auxiliary judgment amount. If the speed of sucking air is too high, bubbles are easily generated, thereby causing inaccurate auxiliary judgment. Therefore, in order to avoid the bubble generation of the common cleaning liquid filled in the liquid path when sucking air, the sucking speed should be controlled to suck slowly. Fig. 3 is a flowchart of a control method of a sample analyzer according to an embodiment of the present invention.
Further, the driver is a syringe or a plunger pump, and the syringe and the plunger pump are driven by a motor to work.
As shown in fig. 3, the control method of the sample analyzer provided in this embodiment may include:
s301, controlling a driver to drive a sampling needle to suck samples from a sample bearing container, wherein the sucking quantity is the sum of the detection dosage and the auxiliary judgment quantity.
S302, judging whether a sample exists on the section where the first bubble sensor is located according to an electric signal output by the first bubble sensor after sample suction is completed, wherein the first bubble sensor is arranged at a preset position on a liquid path pipe connected with a sampling needle. If it is determined that the section where the first bubble sensor is located has a sample according to the electrical signal output by the first bubble sensor, step S303 is executed; if it is determined that there is no sample on the section where the first bubble sensor is located according to the electrical signal output by the first bubble sensor, step S304 is performed. Specifically, when the first bubble sensor is a photoelectric bubble sensor, the relation between the output voltage and the illumination intensity can be obtained according to the volt-ampere characteristic of the photoelectric device of the first bubble sensor through the photoelectric effect of the photoelectric device of the first bubble sensor, and when bubbles exist in the liquid path pipe, the light intensity received by the photosensitive device can be changed due to the fact that light reflection and absorption of different mediums on the light are different, so that the change of the output voltage is caused. The processor can distinguish different media in the liquid path pipe according to the electric signal output by the first bubble sensor, so as to judge whether the section of the first bubble sensor is provided with air or a sample.
And S303, if the section where the first bubble sensor is located has a sample, judging that the sucked detection amount is sufficient, and controlling to supply the sample to the detection module. The sample can be discharged from the sampling needle into the sample-carrying container before the sample is supplied to the detection module, and the discharge amount can be controlled to be an auxiliary determination amount. After the detection module completes the detection, step S305 is executed, and a cleaning process is entered, so as to prepare for the detection of the subsequent sample.
And S304, if no sample exists on the section where the first bubble sensor is located, judging that the sucked detection dosage is insufficient, alarming and not supplying the sample to the detection module, and executing the step S305.
S305, cleaning, including cleaning the sampling needle and the liquid pipeline, so as to prepare for subsequent sample analysis and detection.
According to the control method of the sample analyzer, the driver is controlled to drive the sampling needle to suck samples from the sample bearing container, and the sucking amount is the sum of the detection amount and the auxiliary judgment amount; after the sample suction is finished, judging whether a sample exists on the section where the first bubble sensor is positioned according to an electric signal output by the first bubble sensor, wherein the first bubble sensor is arranged at a preset position on a liquid path pipe connected with a sampling needle; if the section where the first bubble sensor is located is provided with a sample, judging that the sucked detection dosage is sufficient, and controlling the sample to be supplied to the detection module; if no sample exists on the section where the first bubble sensor is located, the insufficient detection dosage is judged to be absorbed, an alarm is given, and no sample is supplied to the detection module. After the sample is sucked, whether the sucked sample amount is sufficient or not is judged through a bubble sensor arranged on the liquid path pipe, so that the cost is low, and misjudgment caused by the conditions that a liquid film exists on the liquid surface, bubbles exist on the liquid film, a sampling needle contacts the container wall, small liquid drops exist on the container wall above the liquid surface and the like can be effectively avoided. In addition, the application can also identify the condition of insufficient sample suction quantity caused by the motion error of the moving mechanism, and avoid the influence on the subsequent detection caused by insufficient sample suction quantity of the sampling needle. Meanwhile, the application does not need to carry out insulation treatment on the sampling needle and adopt a special liquid level sensing circuit board, and the sampling needle is not required to be electrically connected with the liquid level sensing circuit board through a lead, so that the detection cost is low and the control is simple.
On the basis of the above embodiment, the control method of the sample analyzer provided in this embodiment may further include: and controlling the sampling needle to discharge the sample into the sample carrying container, wherein the discharge amount is an auxiliary judgment amount. Specifically, the multi-suction sample is recovered before the sampling needle is controlled to supply the sample to the detection module, specifically, the sampling needle is controlled to discharge the sample into the sample carrying container, and the discharge amount is an auxiliary judgment amount. Through spit out auxiliary judgement volume's sample to sample bearing container in, only leave the sample that detects the quantity and be used for carrying out sample analysis, can avoid sampling needle front end not to have the sample and exist the condition that leaks the drip in the dry chemical detection of urine, can guarantee again that the sample detects the quantity and has avoided the waste of sample.
If bubbles adhere to the wall of the liquid path in the sample sucking process, the first bubble sensor may be misjudged, and in order to further improve the judging accuracy, a compact mounting manner of two bubble sensors may be adopted, so as to avoid misjudgment of the first bubble sensor caused by the above situation. For a sample analyzer employing two compact mounting modes of bubble sensors, the following is specific: the second bubble sensor is arranged on the liquid path pipe, and the distance between the second bubble sensor and the sampling needle is smaller than the distance between the first bubble sensor and the sampling needle. The control method of the sample analyzer may further include: when a sample exists on the section where one of the first bubble sensor and the second bubble sensor is located, the sufficient detection amount is judged to be absorbed. That is, only one bubble sensor is needed to judge that the sample exists, the sucked detection dosage is judged to be sufficient, and the sample can be normally supplied to the detection module.
The auxiliary determination amount may be determined according to the content of the sampling needle and the position of the first bubble sensor. Fig. 4 is a flowchart of a control method of a sample analyzer according to another embodiment of the present invention. As shown in fig. 4, in the control method of the sample analyzer provided in this embodiment, before performing sample detection, the auxiliary determination amount may be determined according to the following steps:
s401, controlling a driver to drive a sampling needle to suck air.
S402, judging whether a sample exists according to the electric signal output by the first bubble sensor. If not, executing step S404; if yes, step S403 is executed.
S403, if it is determined that the section of the first bubble sensor has a sample according to the electrical signal output by the first bubble sensor, the step S402 is continuously performed.
S404, if it is determined that the section of the first bubble sensor has no sample according to the electrical signal output by the first bubble sensor, the step S405 is continuously executed.
And S405, stopping sample suction, calculating the total sample suction amount according to motor motion pulses of the driver, and determining the total sample suction amount as an auxiliary determination amount.
According to the control method of the sample analyzer, before sample detection is carried out, the driver is controlled to drive the sampling needle to suck air, in the process, whether samples exist on the section where the first bubble sensor is located or not is continuously monitored according to the electric signal output by the first bubble sensor, and when no samples exist on the section, sample suction is stopped, and the suction amount at the moment is determined to be an auxiliary judgment amount. Through the process, the auxiliary judgment quantity of each instrument can be automatically debugged, and the influence of the difference of the sampling needle and the liquid pipeline on the judgment of whether the detection dosage of the subsequent suction is sufficient or not is avoided.
The embodiment of the invention also provides a computer readable storage medium, on which a computer program is stored, the computer program being executed by a processor to implement the technical solution of any of the method embodiments described above.
The various embodiments in this disclosure are described in a progressive manner, and identical and similar parts of the various embodiments are all referred to each other, and each embodiment is mainly described as different from other embodiments.
The scope of the present disclosure is not limited to the above-described embodiments, and it is apparent that various modifications and variations can be made to the present disclosure by those skilled in the art without departing from the scope and spirit of the disclosure. Such modifications and variations are intended to be included herein within the scope of the following claims and their equivalents.

Claims (10)

1. A sample analyzer, comprising: the device comprises a sampling needle, a liquid path pipe, a first bubble sensor, a driver and a processor;
the sampling needle, the liquid path pipe and the driver are connected in sequence, and the first bubble sensor is arranged at a preset position on the liquid path pipe;
The processor is configured to:
controlling the driver to drive the sampling needle to suck a sample from a sample bearing container, wherein the sucking quantity is the sum of the detection dosage and an auxiliary judgment quantity, and the auxiliary judgment quantity is determined according to the content of the sampling needle and the position of the first bubble sensor;
After the sample suction is finished, judging whether a sample exists on the section where the first bubble sensor is positioned according to the electric signal output by the first bubble sensor;
If the section where the first bubble sensor is located is provided with a sample, judging that the sucked detection dosage is sufficient;
and if no sample exists on the section where the first bubble sensor is located, judging that the sucked detection dosage is insufficient.
2. The sample analyzer of claim 1, further comprising a detection module, the processor further configured to:
and if the sucked detection amount is judged to be sufficient, before the sample is controlled to be supplied to the detection module, the sampling needle is controlled to discharge the sample into the sample carrying container, and the discharge amount is the auxiliary judgment amount.
3. The sample analyzer of claim 1, further comprising a second bubble sensor disposed on the liquid path tube, and wherein a distance between the second bubble sensor and the sampling needle is less than a distance between the first bubble sensor and the sampling needle.
4. The sample analyzer of claim 3, wherein the processor is further configured to:
And when the section where the first bubble sensor is positioned is provided with a sample or the section where the second bubble sensor is positioned is provided with a sample, judging that the sucked detection dosage is sufficient.
5. The sample analyzer of any one of claims 1-4, wherein the processor is further configured to determine the auxiliary decision amount by:
And controlling the driver to drive the sampling needle to suck air, continuously monitoring whether a sample exists on the section where the first bubble sensor is positioned according to the electric signal output by the first bubble sensor in the process, stopping sucking the sample when no sample exists on the section is monitored, and determining the sucking amount at the moment as the auxiliary judgment amount.
6. A method for controlling a sample analyzer, comprising:
The control driver drives the sampling needle to absorb the sample from the sample carrying container, wherein the absorption amount is the sum of the detection dosage and the auxiliary judgment amount;
After the sample suction is finished, judging whether a sample exists on the section where the first bubble sensor is positioned according to an electric signal output by the first bubble sensor, wherein the first bubble sensor is arranged at a preset position on a liquid path pipe connected with the sampling needle;
If the section where the first bubble sensor is located is provided with a sample, judging that the sucked detection dosage is sufficient;
and if no sample exists on the section where the first bubble sensor is located, judging that the sucked detection dosage is insufficient.
7. The method of claim 6, wherein if the detected amount of suction is determined to be sufficient, the method further comprises:
And before the sample is supplied to the detection module, the sampling needle is controlled to discharge the sample into the sample carrying container, and the discharge amount is the auxiliary judgment amount.
8. The method of claim 6, wherein the method further comprises:
When a sample exists on the section where at least one of the first bubble sensor and the second bubble sensor is located, the sufficient sucking detection dosage is judged, the second bubble sensor is arranged on the liquid path pipe, and the distance between the second bubble sensor and the sampling needle is smaller than the distance between the first bubble sensor and the sampling needle.
9. The method according to any one of claims 6-8, further comprising determining the auxiliary determination amount according to the steps of:
And controlling the driver to drive the sampling needle to suck air, continuously monitoring whether a sample exists on the section where the first bubble sensor is positioned according to the electric signal output by the first bubble sensor in the process, stopping sucking the sample when no sample exists on the section is monitored, and determining the sucking amount at the moment as the auxiliary judgment amount.
10. A computer readable storage medium having stored therein computer executable instructions which when executed by a processor are adapted to carry out the method of controlling a sample analyser according to any of claims 6 to 9.
CN202410377214.4A 2024-03-29 2024-03-29 Sample analyzer and control method thereof Pending CN118393164A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN202410377214.4A CN118393164A (en) 2024-03-29 2024-03-29 Sample analyzer and control method thereof

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN202410377214.4A CN118393164A (en) 2024-03-29 2024-03-29 Sample analyzer and control method thereof

Publications (1)

Publication Number Publication Date
CN118393164A true CN118393164A (en) 2024-07-26

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Family Applications (1)

Application Number Title Priority Date Filing Date
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Country Status (1)

Country Link
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