CN118389242B - Cell screening device - Google Patents
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Abstract
Description
技术领域Technical Field
本发明涉及细胞筛选技术领域,尤其涉及一种细胞筛选装置。The present invention relates to the technical field of cell screening, and in particular to a cell screening device.
背景技术Background Art
细胞筛选是一种将不同类型的细胞或细胞亚群从混合物中分离出来的过程,通过细胞筛选可以获得纯净的细胞群,以进行进一步的研究或应用,目前常见的细胞筛选装置有流式细胞仪、细胞分选系统、磁珠分选系统以及微流控芯片等,其中以流式细胞仪使用较为常见。Cell screening is a process of separating different types of cells or cell subpopulations from a mixture. Through cell screening, pure cell populations can be obtained for further research or application. Currently, common cell screening devices include flow cytometers, cell sorting systems, magnetic bead sorting systems, and microfluidic chips, among which flow cytometers are more commonly used.
流式细胞仪的工作原理是通过将细胞悬浮在液体中,并通过鞘液对细胞的包被作用,使得细胞依次排列逐步经过激光束,当单个细胞经过激光束时,可依据细胞的大小、形状、表面标记和内部荧光等特性,测量并分析每个细胞,从而进行单个细胞的分析和分选。The working principle of flow cytometer is to suspend cells in liquid and coat the cells with sheath fluid so that the cells are arranged in sequence and gradually pass through the laser beam. When a single cell passes through the laser beam, each cell can be measured and analyzed based on its size, shape, surface markers, internal fluorescence and other characteristics, thereby performing single cell analysis and sorting.
在流式细胞仪使用过程中,需要避免气泡进入流动室中导致细胞发生偏移,影响激光束与细胞的正交状态,而目前气泡一般都是通过鞘液混入流动室中,因此对于鞘液中气泡的去除是在进行细胞筛选时重要的步骤,但现有的流式细胞仪一般不具备去除鞘液中气泡的功能,导致在筛选过程中的检测结果存在偏差,因此需要设计一种细胞筛选装置。During the use of flow cytometer, it is necessary to prevent bubbles from entering the flow chamber, causing cells to shift and affecting the orthogonal state of the laser beam and cells. Currently, bubbles are generally mixed into the flow chamber through the sheath fluid. Therefore, removing bubbles in the sheath fluid is an important step in cell screening. However, existing flow cytometers generally do not have the function of removing bubbles in the sheath fluid, resulting in deviations in the detection results during the screening process. Therefore, it is necessary to design a cell screening device.
发明内容Summary of the invention
针对现有技术的不足,本发明提供了一种细胞筛选装置,解决了上述背景技术中提出的鞘液中存在的气泡对检测结果造成影响的问题。In view of the deficiencies of the prior art, the present invention provides a cell screening device, which solves the problem that the bubbles in the sheath fluid affect the detection results as mentioned in the above background technology.
为了实现上述目的,本发明采用了如下技术方案:In order to achieve the above object, the present invention adopts the following technical solutions:
一种细胞筛选装置,包括安装在机体上的鞘液进管、细胞悬液管以及流动室,还包括:A cell screening device, comprising a sheath liquid inlet tube, a cell suspension tube and a flow chamber installed on a body, and also comprising:
缓冲部,所述缓冲部包括固定连通在鞘液进管上的缓冲管,所述缓冲管远离鞘液进管的一端固定连通有鞘液出管,所述鞘液出管另一端固定连通有喷嘴超声振荡器,且喷嘴超声振荡器与流动室固定连通,所述缓冲管内固定安装有隔板;A buffer part, the buffer part includes a buffer tube fixedly connected to the sheath liquid inlet tube, one end of the buffer tube away from the sheath liquid inlet tube is fixedly connected to the sheath liquid outlet tube, the other end of the sheath liquid outlet tube is fixedly connected to the nozzle ultrasonic oscillator, and the nozzle ultrasonic oscillator is fixedly connected to the flow chamber, and a partition is fixedly installed in the buffer tube;
气泡去除部,所述气泡去除部包括固定连通在缓冲管上的导管以及细管,所述导管与细管之间固定连通有文丘里管,且文丘里管上安装有搅动组件,所述细管上固定连通有单向排气管,所述单向排气管上安装有排气组件;A bubble removal unit, the bubble removal unit comprising a conduit fixedly connected to the buffer tube and a capillary tube, a venturi tube fixedly connected between the conduit and the capillary tube, a stirring assembly installed on the venturi tube, a one-way exhaust pipe fixedly connected to the capillary tube, an exhaust assembly installed on the one-way exhaust pipe;
pH测定部,所述pH测定部包括pH探针,所述缓冲管上固定安装有电推杆,且电推杆的输出端与pH探针之间固定安装有固定块,所述缓冲管上安装有与电推杆相配合的控制组件,且控制组件与搅动组件相配合。The pH measuring part includes a pH probe, an electric push rod is fixedly installed on the buffer tube, and a fixed block is fixedly installed between the output end of the electric push rod and the pH probe, a control component matched with the electric push rod is installed on the buffer tube, and the control component matches with the stirring component.
进一步的,所述鞘液进管以及导管均位于隔板左侧,所述细管以及鞘液出管均位于隔板右侧,所述导管的直径大于细管的直径。Furthermore, the sheath fluid inlet tube and the catheter are both located on the left side of the partition, the capillary tube and the sheath fluid outlet tube are both located on the right side of the partition, and the diameter of the catheter is greater than the diameter of the capillary tube.
进一步的,所述搅动组件包括密封转动连接在文丘里管上的转杆,所述转杆位于文丘里管内的一端固定安装有多个叶轮。Furthermore, the stirring assembly includes a rotating rod which is sealingly rotatably connected to the venturi tube, and a plurality of impellers are fixedly mounted on one end of the rotating rod which is located in the venturi tube.
进一步的,所述排气组件包括密封转动连接在单向排气管上的转轴,且转轴位于单向排气管内的一端固定安装有多个排气扇叶。Furthermore, the exhaust assembly includes a rotating shaft which is sealingly rotatably connected to the one-way exhaust pipe, and a plurality of exhaust fan blades are fixedly mounted on one end of the rotating shaft which is located in the one-way exhaust pipe.
进一步的,所述转杆以及转轴位于文丘里管和单向排气管外的一端之间安装有带传动结构。Furthermore, a belt transmission structure is installed between the rotating rod and the rotating shaft located between the venturi tube and one end outside the one-way exhaust pipe.
进一步的,所述带传动结构由带轮一、带轮二以及皮带组成,所述带轮一固定安装在转杆上,所述带轮二固定安装在转轴上,所述皮带套接在带轮一与带轮二之间,所述带轮一的直径大于带轮二的直径。Furthermore, the belt transmission structure consists of pulley one, pulley two and a belt, pulley one is fixedly mounted on a rotating rod, pulley two is fixedly mounted on a rotating shaft, the belt is sleeved between pulley one and pulley two, and the diameter of pulley one is greater than the diameter of pulley two.
进一步的,所述控制组件包括固定安装在缓冲管上的支杆,且支杆上滑动安装有固定齿条,所述转杆远离带轮一的一端固定安装有固定齿圈,且固定齿圈与固定齿条相啮合,所述支杆上固定安装有凸块,且凸块底部固定安装有与电推杆电性连接的触动开关,所述固定齿条顶部固定安装有与触动开关位置相对应的触动块。Furthermore, the control component includes a support rod fixedly mounted on the buffer tube, and a fixed rack is slidably mounted on the support rod, a fixed gear ring is fixedly mounted on the end of the rotating rod away from the pulley, and the fixed gear ring is meshed with the fixed rack, a protrusion is fixedly mounted on the support rod, and a touch switch electrically connected to the electric push rod is fixedly mounted at the bottom of the protrusion, and a touch block corresponding to the position of the touch switch is fixedly mounted on the top of the fixed rack.
进一步的,所述固定齿条的侧壁上固定安装有T形块,所述支杆的侧壁上开设有与T形块相配合的滑槽,所述滑槽与T形块之间安装有弹簧。Furthermore, a T-shaped block is fixedly mounted on the side wall of the fixed rack, a sliding groove matching with the T-shaped block is provided on the side wall of the support rod, and a spring is installed between the sliding groove and the T-shaped block.
进一步的,所述触动开关与触动块的最大间距等于固定齿条的长度。Furthermore, the maximum distance between the touch switch and the touch block is equal to the length of the fixed rack.
进一步的,所述缓冲管上开设有通孔,且通孔内固定安装有与pH探针密封滑动连接的密封圈。Furthermore, a through hole is provided on the buffer tube, and a sealing ring is fixedly installed in the through hole and is sealingly and slidably connected to the pH probe.
与现有的技术相比,本发明优点在于:Compared with the prior art, the present invention has the following advantages:
1:该细胞筛选装置具有去除鞘液中气泡的优点,避免气泡混入流动室内对细胞筛选结果造成影响,具体通过缓冲部与气泡去除部的配合,可对进入缓冲管的鞘液进行去气泡处理。1: The cell screening device has the advantage of removing bubbles in the sheath fluid, thereby preventing bubbles from mixing into the flow chamber and affecting the cell screening results. Specifically, through the cooperation of the buffer part and the bubble removal part, the sheath fluid entering the buffer tube can be debubbled.
2:该细胞筛选装置具有自动化程度高的优点,可在鞘液流动过程中自动完成对其的去气泡处理,具体通过搅动组件与排气组件的配合,可通过鞘液的流速变化为其搅动以及去气泡提供稳定驱动力,无需额外控制。2: The cell screening device has the advantage of a high degree of automation, and can automatically complete the debubbling process of the sheath fluid during its flow. Specifically, through the cooperation of the stirring component and the exhaust component, the flow rate change of the sheath fluid can provide a stable driving force for its stirring and debubbling without the need for additional control.
3:该细胞筛选装置具有自动测定鞘液pH值的优点,确保使用的鞘液处于指定的pH值范围,保证细胞筛选操作顺利进行,具体通过pH测定部的设计,可在搅动组件运行过程中自动完成对鞘液pH值的测定。3: The cell screening device has the advantage of automatically measuring the pH value of the sheath fluid, ensuring that the sheath fluid used is within the specified pH range and ensuring smooth cell screening operations. Specifically, through the design of the pH measuring part, the pH value of the sheath fluid can be automatically measured during the operation of the stirring component.
综上所述,本发明可在鞘液流动过程中利用鞘液流速变化自动完成对其的搅动、去气泡以及pH值测定处理,保证鞘液成分混合均匀,避免其内气泡混入流动室内对细胞筛选结果造成影响,确保了细胞筛选效果,整体自动化程度高,结构简单。In summary, the present invention can utilize the change in the sheath liquid flow rate during the flow of the sheath liquid to automatically complete the stirring, degassing and pH value measurement processing, thereby ensuring that the sheath liquid components are mixed evenly, preventing the bubbles in the sheath liquid from mixing into the flow chamber and affecting the cell screening results, thereby ensuring the cell screening effect, with a high overall degree of automation and a simple structure.
附图说明BRIEF DESCRIPTION OF THE DRAWINGS
图1为本发明提出的一种细胞筛选装置的结构示意图;FIG1 is a schematic structural diagram of a cell screening device proposed by the present invention;
图2为图1中鞘液进管及其连接组件的结构放大示意图;FIG2 is an enlarged schematic diagram of the structure of the sheath liquid inlet tube and its connecting components in FIG1 ;
图3为图2向下偏转一定角度后的结构示意图;FIG3 is a schematic diagram of the structure of FIG2 after being deflected downward by a certain angle;
图4为图3去除流动室及其连接组件后的结构示意图;FIG4 is a schematic diagram of the structure of FIG3 after removing the flow chamber and its connecting components;
图5为图4中缓冲管的内部结构示意图;FIG5 is a schematic diagram of the internal structure of the buffer tube in FIG4 ;
图6为图5中缓冲管及其上端组件的结构放大示意图;FIG6 is an enlarged schematic diagram of the structure of the buffer tube and its upper end assembly in FIG5;
图7为图6去除缓冲管后的结构放大示意图;FIG7 is an enlarged schematic diagram of the structure of FIG6 after the buffer tube is removed;
图8为图7去除文丘里管及其组件后结构放大示意图;FIG8 is an enlarged schematic diagram of the structure of FIG7 after the Venturi tube and its components are removed;
图9为图8的背面结构示意图;FIG9 is a schematic diagram of the back structure of FIG8 ;
图10为图8中固定齿圈及其连接组件的结构放大示意图。FIG. 10 is an enlarged schematic diagram of the structure of the fixed gear ring and its connecting components in FIG. 8 .
图中:1、机体;2、鞘液进管;3、鞘液出管;4、细胞悬液管;5、喷嘴超声振荡器;6、流动室;7、缓冲管;8、隔板;9、导管;10、细管;11、文丘里管;12、转杆;13、叶轮;14、单向排气管;15、转轴;16、排气扇叶;17、带传动结构;18、通孔;19、pH探针;20、固定块;21、电推杆;22、密封圈;23、支杆;24、滑槽;25、T形块;26、固定齿条;27、触动块;28、凸块;29、触动开关;30、固定齿圈;31、弹簧。In the figure: 1, body; 2, sheath liquid inlet tube; 3, sheath liquid outlet tube; 4, cell suspension tube; 5, nozzle ultrasonic oscillator; 6, flow chamber; 7, buffer tube; 8, partition; 9, catheter; 10, capillary; 11, venturi tube; 12, rotating rod; 13, impeller; 14, one-way exhaust pipe; 15, rotating shaft; 16, exhaust fan blade; 17, belt transmission structure; 18, through hole; 19, pH probe; 20, fixed block; 21, electric push rod; 22, sealing ring; 23, support rod; 24, slide groove; 25, T-block; 26, fixed rack; 27, touch block; 28, bump; 29, touch switch; 30, fixed gear ring; 31, spring.
具体实施方式DETAILED DESCRIPTION
参照图1,一种细胞筛选装置,包括安装在机体1上的鞘液进管2、细胞悬液管4以及流动室6,还包括:1 , a cell screening device includes a sheath liquid inlet tube 2, a cell suspension tube 4 and a flow chamber 6 installed on a body 1, and further includes:
缓冲部,缓冲部用于利用鞘液的输入压力对其搅动以及气泡去除操作提供驱动,缓冲部包括固定连通在鞘液进管2上的缓冲管7,气泡去除部,气泡去除部用于对鞘液中存在的气泡进行去除,气泡去除部包括固定连通在缓冲管7上的导管9以及细管10,pH测定部,pH测定部用于对鞘液的pH值进行测定,pH测定部包括pH探针19。A buffer section, the buffer section is used to utilize the input pressure of the sheath liquid to provide drive for its stirring and bubble removal operations, the buffer section includes a buffer tube 7 fixedly connected to the sheath liquid inlet tube 2, a bubble removal section, the bubble removal section is used to remove bubbles existing in the sheath liquid, the bubble removal section includes a catheter 9 and a capillary 10 fixedly connected to the buffer tube 7, and a pH measuring section, the pH measuring section is used to measure the pH value of the sheath liquid, and the pH measuring section includes a pH probe 19.
缓冲管7远离鞘液进管2的一端固定连通有鞘液出管3,鞘液出管3另一端固定连通有喷嘴超声振荡器5,且喷嘴超声振荡器5与流动室6固定连通,鞘液通过鞘液进管2进入缓冲管7中,经过搅动以及去气泡处理后通过鞘液出管3进入喷嘴超声振荡器5,在喷嘴超声振荡器5的作用下将鞘液中的各种成分彻底混合和均匀悬浮,确保在实验过程中细胞悬液与鞘液充分混合,同时喷嘴超声振荡器5还能对鞘液进行进一步的气泡处理工作,保证检测结果的准确度。One end of the buffer tube 7 away from the sheath liquid inlet tube 2 is fixedly connected to the sheath liquid outlet tube 3, and the other end of the sheath liquid outlet tube 3 is fixedly connected to the nozzle ultrasonic oscillator 5, and the nozzle ultrasonic oscillator 5 is fixedly connected to the flow chamber 6. The sheath liquid enters the buffer tube 7 through the sheath liquid inlet tube 2, and after being stirred and debubbled, enters the nozzle ultrasonic oscillator 5 through the sheath liquid outlet tube 3. Under the action of the nozzle ultrasonic oscillator 5, various components in the sheath liquid are thoroughly mixed and evenly suspended, ensuring that the cell suspension and the sheath liquid are fully mixed during the experiment. At the same time, the nozzle ultrasonic oscillator 5 can also perform further bubble processing on the sheath liquid to ensure the accuracy of the detection results.
参照图1-图10,缓冲管7内固定安装有隔板8,隔板8将缓冲管7分为两个部分,靠近鞘液进管2的一部分为进入腔、靠近鞘液出管3的一部分为排出腔,通过鞘液进管2进入缓冲管7内的鞘液先在进入腔内积蓄,随后通过导管9、文丘里管11以及细管10进入排出腔中,之后再通过鞘液出管3进入喷嘴超声振荡器5中并与细胞悬液混合,鞘液进管2以及导管9均位于隔板8左侧,细管10以及鞘液出管3均位于隔板8右侧,导管9的直径大于细管10的直径。Referring to Figures 1 to 10, a partition 8 is fixedly installed in the buffer tube 7, and the partition 8 divides the buffer tube 7 into two parts. A part close to the sheath liquid inlet tube 2 is an entrance cavity, and a part close to the sheath liquid outlet tube 3 is a discharge cavity. The sheath liquid entering the buffer tube 7 through the sheath liquid inlet tube 2 is first accumulated in the entrance cavity, and then enters the discharge cavity through the catheter 9, the Venturi tube 11 and the capillary 10, and then enters the nozzle ultrasonic oscillator 5 through the sheath liquid outlet tube 3 and mixes with the cell suspension. The sheath liquid inlet tube 2 and the catheter 9 are both located on the left side of the partition 8, and the capillary 10 and the sheath liquid outlet tube 3 are both located on the right side of the partition 8. The diameter of the catheter 9 is larger than the diameter of the capillary 10.
导管9与细管10之间固定连通有文丘里管11,文丘里管11的设计,可使得从导管9流入细管10的鞘液流速增大,且文丘里管11上安装有搅动组件,搅动组件包括密封转动连接在文丘里管11上的转杆12,转杆12位于文丘里管11内的一端固定安装有多个叶轮13,当鞘液在较大流速流动时,可通过其对叶轮13的冲击使得转杆12进行转动,同时鞘液与叶轮13的冲击可对鞘液进行一定的搅拌作用,并且鞘液与叶轮13冲击时其流速也会发生变化,而该部分的流速变化也会使得相遇部分形成一个混合区,混合区内的鞘液也会有起伏波动,提高鞘液内成分混合效果。A venturi tube 11 is fixedly connected between the catheter 9 and the capillary tube 10. The design of the venturi tube 11 can increase the flow rate of the sheath liquid flowing from the catheter 9 into the capillary tube 10, and a stirring assembly is installed on the venturi tube 11. The stirring assembly includes a rotating rod 12 that is sealed and rotatably connected to the venturi tube 11. A plurality of impellers 13 are fixedly installed on one end of the rotating rod 12 located in the venturi tube 11. When the sheath liquid flows at a relatively high flow rate, the rotating rod 12 can be rotated by its impact on the impeller 13. At the same time, the impact of the sheath liquid and the impeller 13 can stir the sheath liquid to a certain extent. When the sheath liquid impacts the impeller 13, its flow rate will also change. The change in the flow rate of this part will also form a mixing zone at the meeting part. The sheath liquid in the mixing zone will also fluctuate, thereby improving the mixing effect of the components in the sheath liquid.
细管10上固定连通有单向排气管14,单向排气管14的作用让的气体仅可通过单向排气管14向外排出,单向排气管14上安装有排气组件,排气组件包括密封转动连接在单向排气管14上的转轴15,且转轴15位于单向排气管14内的一端固定安装有多个排气扇叶16,转杆12以及转轴15位于文丘里管11和单向排气管14外的一端之间安装有带传动结构17,在转杆12因叶轮13发生转动时,通过带传动结构17的作用使得转轴15同时转动,此时单向排气管14内产生负压,使得鞘液中的气泡通过单向排气管14向外排出,实现气泡去除效果。A one-way exhaust pipe 14 is fixedly connected to the capillary 10. The one-way exhaust pipe 14 allows the gas to be discharged outward only through the one-way exhaust pipe 14. An exhaust assembly is installed on the one-way exhaust pipe 14, and the exhaust assembly includes a rotating shaft 15 which is sealed and rotatably connected to the one-way exhaust pipe 14, and a plurality of exhaust fan blades 16 are fixedly installed at one end of the rotating shaft 15 located in the one-way exhaust pipe 14. A belt drive structure 17 is installed between the rotating rod 12 and the end of the rotating shaft 15 located outside the venturi tube 11 and the one-way exhaust pipe 14. When the rotating rod 12 rotates due to the impeller 13, the rotating shaft 15 rotates at the same time through the action of the belt drive structure 17. At this time, negative pressure is generated in the one-way exhaust pipe 14, so that the bubbles in the sheath liquid are discharged outward through the one-way exhaust pipe 14, thereby achieving the bubble removal effect.
带传动结构17由带轮一、带轮二以及皮带组成,带轮一固定安装在转杆12上,带轮二固定安装在转轴15上,皮带套接在带轮一与带轮二之间,带轮一的直径大于带轮二的直径,通过带轮一与带轮二的直径差异,使得转杆12带动转轴15转动时的速度更快,从而可在一定程度上提高单向排气管14的排气效果。The belt drive structure 17 consists of a pulley 1, a pulley 2 and a belt. The pulley 1 is fixedly mounted on the rotating rod 12, the pulley 2 is fixedly mounted on the rotating shaft 15, the belt is sleeved between the pulley 1 and the pulley 2, the diameter of the pulley 1 is greater than the diameter of the pulley 2, and through the diameter difference between the pulley 1 and the pulley 2, the rotating rod 12 drives the rotating shaft 15 to rotate faster, thereby improving the exhaust effect of the one-way exhaust pipe 14 to a certain extent.
缓冲管7上固定安装有电推杆21,且电推杆21的输出端与pH探针19之间固定安装有固定块20,通过电推杆21的设计,可在其工作时使得pH探针19插入缓冲管7内,利用其对缓冲管7内的鞘液pH进行测定,由于pH探针19位于隔板8右侧,因此其测定的鞘液是经过充分混合以及去气泡处理后的,故而此时测定的pH值更为准确。An electric push rod 21 is fixedly installed on the buffer tube 7, and a fixed block 20 is fixedly installed between the output end of the electric push rod 21 and the pH probe 19. Through the design of the electric push rod 21, the pH probe 19 can be inserted into the buffer tube 7 when it is working, and the pH of the sheath liquid in the buffer tube 7 can be measured by it. Since the pH probe 19 is located on the right side of the partition 8, the sheath liquid measured by it is fully mixed and de-bubbled, so the pH value measured at this time is more accurate.
缓冲管7上开设有通孔18,且通孔18内固定安装有与pH探针19密封滑动连接的密封圈22,在密封圈22的设计下,可保证通孔18与pH探针19之间的密封效果,避免外界气体因pH探针19移动进入缓冲管7中。A through hole 18 is provided on the buffer tube 7, and a sealing ring 22 is fixedly installed in the through hole 18 and is sealingly and slidably connected to the pH probe 19. The design of the sealing ring 22 can ensure the sealing effect between the through hole 18 and the pH probe 19, and prevent external gas from moving into the buffer tube 7 due to the pH probe 19.
缓冲管7上安装有与电推杆21相配合的控制组件,且控制组件与搅动组件相配合,控制组件包括固定安装在缓冲管7上的支杆23,且支杆23上滑动安装有固定齿条26,转杆12远离带轮一的一端固定安装有固定齿圈30,且固定齿圈30与固定齿条26相啮合,支杆23上固定安装有凸块28,且凸块28底部固定安装有与电推杆21电性连接的触动开关29,固定齿条26顶部固定安装有与触动开关29位置相对应的触动块27,当触动开关29被触动块27进行触动时,电推杆21开始收缩使得pH探针19插入缓冲管7内部,反之,当触动开关29未被触动时,那么电推杆21则保持伸长状态,使得pH探针19位于缓冲管7外部。A control assembly that cooperates with the electric push rod 21 is installed on the buffer tube 7, and the control assembly cooperates with the stirring assembly. The control assembly includes a support rod 23 fixedly installed on the buffer tube 7, and a fixed rack 26 is slidably installed on the support rod 23. A fixed gear ring 30 is fixedly installed on the end of the rotating rod 12 away from the pulley, and the fixed gear ring 30 is meshed with the fixed rack 26. A protrusion 28 is fixedly installed on the support rod 23, and a touch switch 29 electrically connected to the electric push rod 21 is fixedly installed at the bottom of the protrusion 28. A touch block 27 corresponding to the position of the touch switch 29 is fixedly installed on the top of the fixed rack 26. When the touch switch 29 is touched by the touch block 27, the electric push rod 21 begins to contract so that the pH probe 19 is inserted into the buffer tube 7. Conversely, when the touch switch 29 is not touched, the electric push rod 21 remains in an extended state so that the pH probe 19 is located outside the buffer tube 7.
触动开关29以及pH探针19均为现有技术,其工作原理以及具体结构在此不做阐述。The touch switch 29 and the pH probe 19 are both prior art, and their working principles and specific structures are not described here.
固定齿条26的侧壁上固定安装有T形块25,支杆23的侧壁上开设有与T形块25相配合的滑槽24,滑槽24与T形块25之间安装有弹簧31,在滑槽24与T形块25的配合下,可保证固定齿条26与支杆23之间的连接稳定性,在弹簧31的作用下,可在固定齿条26未受外力时保持触动开关29处于未被触动状态。A T-shaped block 25 is fixedly installed on the side wall of the fixed rack 26, and a slide groove 24 cooperating with the T-shaped block 25 is opened on the side wall of the support rod 23. A spring 31 is installed between the slide groove 24 and the T-shaped block 25. With the cooperation of the slide groove 24 and the T-shaped block 25, the connection stability between the fixed rack 26 and the support rod 23 can be guaranteed. Under the action of the spring 31, the touch switch 29 can be kept in an untouched state when the fixed rack 26 is not subjected to external force.
触动开关29与触动块27的最大间距等于固定齿条26的长度,此处尺寸的设计好处在于,可保证固定齿条26受固定齿圈30推动发生上移时,能在最大移动位置时确保对触动开关29的触动效果,同时此时固定齿条26与固定齿圈30处于即将分离状态,使得固定齿圈30持续转动时不会因固定齿条26移动受限而受到较大转动阻力,确保转杆12的持续顺利转动。The maximum distance between the touch switch 29 and the touch block 27 is equal to the length of the fixed rack 26. The design advantage of the size here is that when the fixed rack 26 is pushed upward by the fixed gear ring 30, the touch switch 29 can be triggered at the maximum movement position. At the same time, the fixed rack 26 and the fixed gear ring 30 are about to separate, so that when the fixed gear ring 30 continues to rotate, it will not be subject to a large rotation resistance due to the limited movement of the fixed rack 26, thereby ensuring the continuous and smooth rotation of the rotating rod 12.
本发明中,一种细胞筛选装置的工作原理包括以下步骤:In the present invention, the working principle of a cell screening device includes the following steps:
样品准备:收集细胞样品,并进行必要的预处理,如细胞培养、离心等,确保样品的质量和纯度,将细胞样品悬浮在适当的缓冲液中支撑细胞悬液,另取一定容积的鞘液备用;Sample preparation: Collect cell samples and perform necessary pretreatment, such as cell culture and centrifugation, to ensure the quality and purity of the samples. Suspend the cell samples in an appropriate buffer to support the cell suspension, and take a certain volume of sheath fluid for standby use;
筛选处理:将细胞悬液通过细胞悬液管4输入流动室6中,将鞘液通过鞘液进管2输入缓冲管7中,并在经过缓冲管7的缓冲处理后通过鞘液出管3进入喷嘴超声振荡器5中与细胞悬液混合;Screening treatment: the cell suspension is input into the flow chamber 6 through the cell suspension tube 4, the sheath liquid is input into the buffer tube 7 through the sheath liquid inlet tube 2, and after the buffer treatment of the buffer tube 7, the sheath liquid is input into the nozzle ultrasonic oscillator 5 through the sheath liquid outlet tube 3 to be mixed with the cell suspension;
在鞘液作用下细胞依次逐个通过机体1内的激光系统,激光束逐个照射细胞,并激发其标记物发出荧光信号,光学系统捕获并记录这些信号,并根据信号的强度和荧光颜色对细胞进行识别和分类;Under the action of sheath fluid, cells pass through the laser system in the body 1 one by one, and the laser beam irradiates the cells one by one, and excites their markers to emit fluorescent signals. The optical system captures and records these signals, and identifies and classifies the cells according to the signal intensity and fluorescence color;
通过激光系统的细胞在不同电荷偏转板的作用下被分类收集,实现筛选处理;The cells passing through the laser system are classified and collected under the action of deflection plates with different charges to achieve screening processing;
鞘液的气泡去除处理:进入缓冲管7的鞘液会通过导管9从文丘里管11中进入细管10内,当鞘液流经文丘里管11时,其流速会增大,此时鞘液对叶轮13进行冲击使得转杆12发生转动,同时叶轮13的转动以及与鞘液的冲击效果可对鞘液进行充分混合,使得鞘液内部成分分布均匀,同时也便于其内气泡析出;Bubble removal process of sheath liquid: the sheath liquid entering the buffer tube 7 will enter the capillary tube 10 from the venturi tube 11 through the conduit 9. When the sheath liquid flows through the venturi tube 11, its flow rate will increase. At this time, the sheath liquid impacts the impeller 13 to make the rotating rod 12 rotate. At the same time, the rotation of the impeller 13 and the impact effect with the sheath liquid can fully mix the sheath liquid, so that the internal components of the sheath liquid are evenly distributed, and it is also convenient for the bubbles in it to precipitate;
转杆12转动时通过带传动结构17带动转轴15高速转动,此时在多个排气扇叶16的作用下从鞘液中析出的气泡通过单向排气管14排出,实现气泡去除操作;When the rotating rod 12 rotates, the rotating shaft 15 is driven to rotate at high speed through the belt transmission structure 17. At this time, the bubbles precipitated from the sheath liquid are discharged through the one-way exhaust pipe 14 under the action of multiple exhaust blades 16, thereby realizing the bubble removal operation;
鞘液的pH值测定:在转杆12转动过程中,固定齿圈30随之同时转动,此时与固定齿圈30啮合的固定齿条26会沿着支杆23上移,并带动其上触动块27对触动开关29进行触动,此时触动开关29发出电信号使得电推杆21收缩,pH探针19插入缓冲管7内,对进行充分混合且去除气泡后的鞘液进行pH的测定处理。Determination of pH value of sheath liquid: During the rotation of the rotating rod 12, the fixed gear ring 30 rotates simultaneously. At this time, the fixed rack 26 meshing with the fixed gear ring 30 will move up along the support rod 23, and drive the trigger block 27 thereon to trigger the trigger switch 29. At this time, the trigger switch 29 sends an electrical signal to cause the electric push rod 21 to contract, and the pH probe 19 is inserted into the buffer tube 7 to measure the pH value of the sheath liquid after it is fully mixed and the bubbles are removed.
以上显示和描述了本发明的基本原理和主要特征和本发明的优点。本行业的技术人员应该了解,本发明不受上述实施例的限制,上述实施例和说明书中描述的只是说明本发明的原理,在不脱离本发明精神和范围的前提下,本发明还会有各种变化和改进,这些变化和改进都落入要求保护的本发明范围内。The above shows and describes the basic principles and main features of the present invention and the advantages of the present invention. It should be understood by those skilled in the art that the present invention is not limited by the above embodiments, and the above embodiments and descriptions are only for explaining the principles of the present invention. Without departing from the spirit and scope of the present invention, the present invention may have various changes and improvements, and these changes and improvements all fall within the scope of the present invention to be protected.
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