CN118272574B - 棉花GaIAA14基因突变位点及应用 - Google Patents
棉花GaIAA14基因突变位点及应用 Download PDFInfo
- Publication number
- CN118272574B CN118272574B CN202410671655.5A CN202410671655A CN118272574B CN 118272574 B CN118272574 B CN 118272574B CN 202410671655 A CN202410671655 A CN 202410671655A CN 118272574 B CN118272574 B CN 118272574B
- Authority
- CN
- China
- Prior art keywords
- gaiaa
- cotton
- gene
- mutation
- sequence
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
- 229920000742 Cotton Polymers 0.000 title claims abstract description 18
- 206010064571 Gene mutation Diseases 0.000 title abstract description 4
- 108090000623 proteins and genes Proteins 0.000 claims description 27
- 230000001105 regulatory effect Effects 0.000 claims description 6
- 230000033228 biological regulation Effects 0.000 claims description 3
- 101150063006 IAA14 gene Proteins 0.000 abstract description 11
- SEOVTRFCIGRIMH-UHFFFAOYSA-N indole-3-acetic acid Chemical compound C1=CC=C2C(CC(=O)O)=CNC2=C1 SEOVTRFCIGRIMH-UHFFFAOYSA-N 0.000 abstract description 10
- 229930192334 Auxin Natural products 0.000 abstract description 8
- 239000002363 auxin Substances 0.000 abstract description 8
- 238000010362 genome editing Methods 0.000 abstract description 6
- 230000035613 defoliation Effects 0.000 abstract description 4
- 238000005096 rolling process Methods 0.000 abstract description 3
- 238000009395 breeding Methods 0.000 abstract description 2
- 230000001488 breeding effect Effects 0.000 abstract description 2
- 238000003306 harvesting Methods 0.000 abstract description 2
- 230000003827 upregulation Effects 0.000 abstract description 2
- 230000001737 promoting effect Effects 0.000 abstract 1
- 230000035772 mutation Effects 0.000 description 32
- 239000000463 material Substances 0.000 description 17
- 241000219146 Gossypium Species 0.000 description 12
- 102000004169 proteins and genes Human genes 0.000 description 9
- 101100313373 Aliivibrio fischeri (strain ATCC 700601 / ES114) tfoX1 gene Proteins 0.000 description 7
- 238000002703 mutagenesis Methods 0.000 description 6
- 231100000350 mutagenesis Toxicity 0.000 description 6
- 238000004458 analytical method Methods 0.000 description 5
- 238000000034 method Methods 0.000 description 5
- 238000012163 sequencing technique Methods 0.000 description 5
- 230000015556 catabolic process Effects 0.000 description 3
- 239000013599 cloning vector Substances 0.000 description 3
- 238000005520 cutting process Methods 0.000 description 3
- 238000006731 degradation reaction Methods 0.000 description 3
- 238000010586 diagram Methods 0.000 description 3
- 238000005516 engineering process Methods 0.000 description 3
- 239000000047 product Substances 0.000 description 3
- 238000013518 transcription Methods 0.000 description 3
- 230000035897 transcription Effects 0.000 description 3
- 241000588724 Escherichia coli Species 0.000 description 2
- 240000002024 Gossypium herbaceum Species 0.000 description 2
- 235000004341 Gossypium herbaceum Nutrition 0.000 description 2
- AGPKZVBTJJNPAG-WHFBIAKZSA-N L-isoleucine Chemical compound CC[C@H](C)[C@H](N)C(O)=O AGPKZVBTJJNPAG-WHFBIAKZSA-N 0.000 description 2
- KZSNJWFQEVHDMF-BYPYZUCNSA-N L-valine Chemical compound CC(C)[C@H](N)C(O)=O KZSNJWFQEVHDMF-BYPYZUCNSA-N 0.000 description 2
- KZSNJWFQEVHDMF-UHFFFAOYSA-N Valine Natural products CC(C)C(N)C(O)=O KZSNJWFQEVHDMF-UHFFFAOYSA-N 0.000 description 2
- 230000015572 biosynthetic process Effects 0.000 description 2
- 230000001276 controlling effect Effects 0.000 description 2
- 238000012217 deletion Methods 0.000 description 2
- 230000037430 deletion Effects 0.000 description 2
- 239000000499 gel Substances 0.000 description 2
- 230000006872 improvement Effects 0.000 description 2
- 229960000310 isoleucine Drugs 0.000 description 2
- AGPKZVBTJJNPAG-UHFFFAOYSA-N isoleucine Natural products CCC(C)C(N)C(O)=O AGPKZVBTJJNPAG-UHFFFAOYSA-N 0.000 description 2
- 238000011160 research Methods 0.000 description 2
- 238000006467 substitution reaction Methods 0.000 description 2
- 239000004474 valine Substances 0.000 description 2
- 241000219194 Arabidopsis Species 0.000 description 1
- 108700014010 Arabidopsis IAA8 Proteins 0.000 description 1
- 240000002791 Brassica napus Species 0.000 description 1
- 235000011293 Brassica napus Nutrition 0.000 description 1
- 241001073507 Callicarpa Species 0.000 description 1
- 241001136615 Callicarpa dichotoma Species 0.000 description 1
- 241000196324 Embryophyta Species 0.000 description 1
- 101150079901 IAA2 gene Proteins 0.000 description 1
- 101150032688 IAA7 gene Proteins 0.000 description 1
- 108700005075 Regulator Genes Proteins 0.000 description 1
- 108090000848 Ubiquitin Proteins 0.000 description 1
- 102000044159 Ubiquitin Human genes 0.000 description 1
- 239000011543 agarose gel Substances 0.000 description 1
- 229940024606 amino acid Drugs 0.000 description 1
- 150000001413 amino acids Chemical class 0.000 description 1
- 230000001580 bacterial effect Effects 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 238000006243 chemical reaction Methods 0.000 description 1
- 239000003153 chemical reaction reagent Substances 0.000 description 1
- 229930002875 chlorophyll Natural products 0.000 description 1
- 235000019804 chlorophyll Nutrition 0.000 description 1
- ATNHDLDRLWWWCB-AENOIHSZSA-M chlorophyll a Chemical compound C1([C@@H](C(=O)OC)C(=O)C2=C3C)=C2N2C3=CC(C(CC)=C3C)=[N+]4C3=CC3=C(C=C)C(C)=C5N3[Mg-2]42[N+]2=C1[C@@H](CCC(=O)OC\C=C(/C)CCC[C@H](C)CCC[C@H](C)CCCC(C)C)[C@H](C)C2=C5 ATNHDLDRLWWWCB-AENOIHSZSA-M 0.000 description 1
- 238000010367 cloning Methods 0.000 description 1
- 230000008645 cold stress Effects 0.000 description 1
- 238000007796 conventional method Methods 0.000 description 1
- 239000008380 degradant Substances 0.000 description 1
- 230000024346 drought recovery Effects 0.000 description 1
- 230000002349 favourable effect Effects 0.000 description 1
- 230000014509 gene expression Effects 0.000 description 1
- 238000010353 genetic engineering Methods 0.000 description 1
- 239000003617 indole-3-acetic acid Substances 0.000 description 1
- 239000003112 inhibitor Substances 0.000 description 1
- 230000011890 leaf development Effects 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 230000007246 mechanism Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 108020001580 protein domains Proteins 0.000 description 1
- 230000004853 protein function Effects 0.000 description 1
- 238000000746 purification Methods 0.000 description 1
- 238000011084 recovery Methods 0.000 description 1
- 230000004044 response Effects 0.000 description 1
- 238000007480 sanger sequencing Methods 0.000 description 1
- 230000035945 sensitivity Effects 0.000 description 1
- 238000011144 upstream manufacturing Methods 0.000 description 1
- 239000013598 vector Substances 0.000 description 1
- 238000012795 verification Methods 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
- C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
- C12Q1/6895—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for plants, fungi or algae
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6869—Methods for sequencing
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2600/00—Oligonucleotides characterized by their use
- C12Q2600/13—Plant traits
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2600/00—Oligonucleotides characterized by their use
- C12Q2600/156—Polymorphic or mutational markers
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Health & Medical Sciences (AREA)
- Wood Science & Technology (AREA)
- Analytical Chemistry (AREA)
- Zoology (AREA)
- Biotechnology (AREA)
- Microbiology (AREA)
- General Engineering & Computer Science (AREA)
- Physics & Mathematics (AREA)
- Biophysics (AREA)
- Immunology (AREA)
- Biochemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Molecular Biology (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Botany (AREA)
- Mycology (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
Abstract
本发明涉及棉花GaIAA14基因突变位点及应用，通过改变IAA14的2号外显子G393A，导致生长素的上调，并产生杯状叶表型，为IAA14特异性基因编辑提供新型候选靶点，促进棉花卷叶品种的选育，可以有效改善棉花群体密度大，易造成冠层郁蔽，影响群体光能利用率，后期脱叶不彻底，不利于机采的问题。
Description
技术领域
本发明涉及基因工程技术领域，具体地说，涉及棉花GaIAA14基因突变位点及应用。
背景技术
在农作物中进行定点可控的基因编辑，有助于快速、高效地获得优良性状的植株。新疆维吾尔自治区是我国棉花的主产区，“密、矮、早”栽培技术被广泛应用。在该种植模式下，群体密度大，易造成冠层郁蔽，影响群体光能利用率，后期脱叶不彻底，不利于机采。因此，针对棉花叶片发育机理开展研究，调控叶片形态，改善冠层结构，提高有效光能利用率和脱叶率，简化栽培措施具有重要意义。
Auxin/indole-3-acetic acid（Aux/IAA）基因家族是生长素的早期响应因子，属于转录抑制因子，负责调控生长素下游基因的转录。生长素浓度高时，AUX/IAA蛋白会形成AUX/IAA-生长素-SCFTIR1/AFB复合物，将泛素连接到AUX/IAA蛋白上，导致AUX/IAA蛋白的降解（WEIJERS et al., 2005; PARRY et al., 2009; TAN et al., 2007）。生长素浓度低时，AUX/IAA蛋白与ARF和TPL形成三元复合物，结合在生长素调控基因的上游启动子上，抑制其转录。AUX/IAA蛋白结构域II具有保守的“GWPPV（I）”基序，该基序被称为降解子，可结合TIR1型SCF并调控AUX/IAA蛋白的降解。AUX/IAA蛋白保守结构域II的降解子“GWPPV（I）”中的氨基酸变异往往导致显性或半显性突变，产生功能获得型突变体。例如甘蓝型油菜IAA2蛋白中“GWPPV”改变为“GWSPV”（TAN et al., 2023）和“GWPSV”（HUANG et al.,2020）、IAA7蛋白中“GWPPV”改变为“EWPPV”（WEI et al., 2021）、“EWLPV”（CHENG et al.,2019）以及拟南芥IAA8蛋白中“GWPPV”改变为“EWPPV”（WANG et al., 2013）都产生了叶片卷曲。前人研究表明，IAA14在侧根形成中发挥重要作用。拟南芥slr突变体IAA14基因发生单碱基变异，导致“GWPPV”改变为“GWPSV”，产生了无侧根、耐旱、冷胁迫敏感、叶绿素生物合成基因表达上调等表型。
调控棉花叶片形态，改善冠层结构，提高有效光能利用率和脱叶率具有重要意义，但目前尚没有通过改变IAA14得到新型棉花品种的相关研究。
发明内容
本发明涉及棉花GaIAA14基因单碱基变异及其应用，通过单碱基改变来调控相应基因/蛋白功能是基因编辑技术的基本要求和目标，目的基因的关键碱基和潜在的突变类型是进行精准基因编辑的基础。本发明通过改变IAA14的2号外显子G393A，导致生长素的上调，并产生杯状叶（卷叶的一种）表型，为IAA14特异性基因编辑提供新型候选靶点，促进卷叶品种的选育。
本发明的技术方案如下：
一种与调控棉花叶片形态相关的基因突变位点，其特征在于，所述突变为位点为IAA14基因的2号外显子第393位G＞A位点突变。
优选的是，所述棉花叶片形态为杯状叶形态。
IAA14基因第393位G＞A位点突变在棉花株型改良中的应用。
确定与调控棉花叶片形态相关的基因突变位点的方法，包括如下步骤：
EMS诱变材料CU及野生型材料SXY1中扩增Ga03G0409(GaIAA14) 的基因组序列，将所得PCR产物切胶回收，连接转化至TOPO平末端克隆载体，进行测序分析，得到突变位点并对突变位点进行分析，再对突变表型鉴定。
优选的是，使用体积分数0.6%的EMS溶液在28 ℃下黑暗处理野生型材料SXY1种子8 h，播种于大田，经多代自交纯化，获得稳定遗传的杯状叶突变体材料CU。
优选的是，PCR引物如序列1和序列2所示。使用KOD One TM PCR Master Mix-Blue-分别在EMS诱变材料CU及野生型材料SXY1中扩增Ga03G0409(GaIAA14) 的基因组序列。
PCR引物：
序列1 09F: ATGCTAGGAACTGAGCATGATTTGA
序列2 09R：TTAGACTCTGCTCTTGCATTTCTCC
优选的是，杯状叶基因GaIAA14，其基因组DNA (1559bp) 如序列3所示，CDS(747bp) 如序列4所示，GaIAA14单碱基G393A突变后基因组DNA (1559bp) 如序列5所示，CDS （747bp) 如序列6所示。
上述任一方案优选的是，单碱基G393A突变位于 GaIAA14的2号外显子，导致CDS发生由G到A的单碱基变异，导致 GaIAA14蛋白发生了由缬氨酸（V）到异亮氨酸（I）的替换，在三维构象上缺失了一段α螺旋。
上述任一方案优选的是，单碱基G393A突变导致CU突变体杯状叶表型。
为了鉴定有利于棉花株型改良的IAA14单碱基突变位点，本发明通过EMS诱变获取的一株亚洲棉杯状叶材料，鉴定到了IAA14基因的一个新型突变位点，可应用于基因编辑技术，定向形成杯状叶，创制适宜于密植的材料，大幅提高生产效率。
附图说明
为了更清楚地说明本发明实施例的技术方案，下面将对实施例中所需要使用的附图简单地介绍，应当理解，以下附图仅示出了本发明的实施例，因此不应被看作是对范围的限定，对于本领域普通技术人员来讲，在不付出创造性劳动的前提下，还可以根据这些附图获得其他相关附图。
图1为突变体CU与野生型SXY1中GaIAA14基因组序列比对图；
图2为突变体CU与野生型SXY1中GaIAA14基因CDS序列比对图；
图3为GaIAA14基因G393A突变导致CDS变异位点图；
图4为G393A突变导致GaIAA14蛋白α螺旋部分缺失结构图；
图5为单碱基G393A突变导致CU突变体杯状叶表型图。
具体实施方式
下述实施例中所使用的方法如无特殊说明，均为常规方法。
下述实施例中所用的材料、试剂等，如无特殊说明均可从商业途径获得。
实施例
本发明确定与调控棉花叶片形态相关的基因突变位点的方法为：在EMS诱变材料CU及野生型材料SXY1中扩增Ga03G0409(GaIAA14) 的基因组序列，将所得PCR产物切胶回收，连接转化至TOPO平末端克隆载体，进行测序分析，得到突变位点并对突变位点进行分析，再对突变表型鉴定。
（1）GaIAA4单碱基G393A突变测序验证
使用体积分数0.6%的EMS溶液在28 ℃下黑暗处理野生型材料SXY1种子8 h，播种于大田，经多代自交纯化，获得稳定遗传的杯状叶突变体材料CU。如图1所示，使用KOD OneTM PCR Master Mix-Blue-分别在EMS诱变材料CU及野生型材料SXY1中扩增Ga03G0409(GaIAA14) 的基因组序列。
PCR引物：
序列109F: ATGCTAGGAACTGAGCATGATTTGA
序列209R：TTAGACTCTGCTCTTGCATTTCTCC
将所得PCR产物切胶回收（步骤详见天根DP209-02/DP209-03普通琼脂糖凝胶DNA回收试剂盒说明书），连接至TOPO二代平末端克隆载体（5 min TA/Blunt-Zero CloningKit，25 ℃下反应5min），载体转化大肠杆菌，将大肠杆菌菌液送至擎科生物进行Sanger测序分析。结果表明，EMS诱变材料CU的GaIAA14基因第393bp处发生了由G至A的突变。同理进一步在上述材料中扩增Ga03G0409(GaIAA14)的CDS序列，测序结果如图2所示，G393A单碱基突变后Ga03G0409(GaIAA14)的CDS序列出现由G至A的突变。
（2）GaIAA14单碱基G393A突变位点分析
结合测序结果发现，单碱基G393A突变位于GaIAA14的2号外显子，导致CDS发生由G到A的单碱基变异 (图3)，导致GaIAA14蛋白发生了由缬氨酸（V）到异亮氨酸（I）的替换，在三维构象上缺失了一段α螺旋 (图4)。
（3）GaIAA14单碱基G393A突变表型鉴定
CU突变体中GaIAA14基因发生单碱基G393A突变，与野生型SXY1相比，CU突变体叶片的边缘向内向上卷曲，呈现杯状(图5中的C)，CU突变体子叶就呈现杯状(图5中的B)，整株所有真叶也都呈现杯状(图5中的A)。
本发明中杯状叶基因GaIAA14，其基因组DNA (1559bp) 如序列3所示，CDS(747bp) 如序列4所示，GaIAA14单碱基G393A突变后基因组DNA (1559bp) 如序列5所示，CDS（747bp) 如序列6所示。
序列3：
5’-ATGCTAGGAACTGAGCATGATTTGAACTTCAAAGAGACCGAGCTGTGTCTCGGCCTGCCTGGTGGAAGTGGTGGAGGAGGAGGAGCTGCCGCCGTCGGTGGTGGTGGTAAAAATGAAGTGGAAAGTTCCCCAAAGACCACCGGGAAAAGAGGCTACTCCGAGACTGTTGATTTGAAGCTCAATCTTCAGTCTAAAGAAGGTTGCATGGATCTGAATCAGAACCTAGAAAATGGTTCAAAAGAAAAGAATCATCTCCCTGCAAAACCACCAGCCAAgtatgtcaaaaaaaaaactaccattgaaacatattatatttgttttttttctatatttgtgtgtatatattgatttgtggttggattttaagGGCACAAGTATTGGGTTGGCCACCAGTTCGATCTTATAGGAAGAACATTATGGCTAACCAGAAGAACAACAGTGACGAAATTAGTGACAGAGCTAGCAGTGGCAGTGGGGGTGCAGCGTTTGTGAAGGTTTGCATGGATGGTGCACCATATTTGCGTAAAGTAGATTTGAAGATGTACAACAGCTACCAAAAGTTATCCGATGCCTTGGCTAAGATGTTCAGTTCCTTCACCATGGgtaagttttacggttcgaaccttaacatgcagcttagggttgatacttcatcatcatggatagatataacatttgttttttaagacaatgatatgatattttgagggttgtaatagtaagaaatctgagtaacttggtgggtttttttttttttttgaagGGAGTTATGGATGTGAAGGAATGATAGACTTCATGAATGAGAGCAAGTTGATGGATCTTCTCAACAGTTCTGATTATGTGCCAACCTATGAAGATAAGGATGGCGACTGGATGCTCGTGGGCGATGTCCCATGGGAgtatgtttcacatattttatatatatatatatatgcatgctagtcttcattagatatctccccacagtcatgcatgcacatcttttagcagctcacctcacctacaaattaatctgctttcctttcgatatatattctagtacacccagttaggtgaaaaaattaacatttctagtatataattgagtttttattaatttattaaacgtaaaatgataaaaatattttcataattatataacttactttatacataaaagaatgttttaataatttctcagattaagttagtgtccgattgacacgtaacattaactcaattatgaacttaatataattataaatatagataaaagttgatgtacttatagtacgtaattctatgcacttaacatatctacatcaaaaaattttaaatctaaatctacttaacttcattttttaacaaaatctcatcattttatttcttattttttcacagGATGTTTGTTGAATCATGTAAACGTTTGCGCATAATGAAAGGATCAGAGGCAATAGGGCTTGgtacgtttgattttttatttatatacatacatgaattcaattcacaaatgtaaagttaatttgttgatattaatttcagCTCCAAGAGCAATGGAGAAATGCAAGAGCAGAGTCTAA-3’
序列4：
5’-ATGCTAGGAACTGAGCATGATTTGAACTTCAAAGAGACCGAGCTGTGTCTCGGCCTGCCTGGTGGAAGTGGTGGAGGAGGAGGAGCTGCCGCCGTCGGTGGTGGTGGTAAAAATGAAGTGGAAAGTTCCCCAAAGACCACCGGGAAAAGAGGCTACTCCGAGACTGTTGATTTGAAGCTCAATCTTCAGTCTAAAGAAGGTTGCATGGATCTGAATCAGAACCTAGAAAATGGTTCAAAAGAAAAGAATCATCTCCCTGCAAAACCACCAGCCAAGGCACAAGTATTGGGTTGGCCACCAGTTCGATCTTATAGGAAGAACATTATGGCTAACCAGAAGAACAACAGTGACGAAATTAGTGACAGAGCTAGCAGTGGCAGTGGGGGTGCAGCGTTTGTGAAGGTTTGCATGGATGGTGCACCATATTTGCGTAAAGTAGATTTGAAGATGTACAACAGCTACCAAAAGTTATCCGATGCCTTGGCTAAGATGTTCAGTTCCTTCACCATGGGGAGTTATGGATGTGAAGGAATGATAGACTTCATGAATGAGAGCAAGTTGATGGATCTTCTCAACAGTTCTGATTATGTGCCAACCTAT
GAAGATAAGGATGGCGACTGGATGCTCGTGGGCGATGTCCCATGGGAGATGTTTGTTGAATCATGTAAACGTTTGCGCATAATGAAAGGATCAGAGGCAATAGGGCTTGCTCCAAGAGCAATGGAGAAATGCAAGAGCAGAGTCTAA-3’
序列5：
5’-ATGCTAGGAACTGAGCATGATTTGAACTTCAAAGAGACCGAGCTGTGTCTCGGCCTGCCTGGTGGAAGTGGTGGAGGAGGAGGAGCTGCCGCCGTCGGTGGTGGTGGTAAAAATGAAGTGGAAAGTTCCCCAAAGACCACCGGGAAAAGAGGCTACTCCGAGACTGTTGATTTGAAGCTCAATCTTCAGTCTAAAGAAGGTTGCATGGATCTGAATCAGAACCTAGAAAATGGTTCAAAAGAAAAGAATCATCTCCCTGCAAAACCACCAGCCAAgtatgtcaaaaaaaaaactaccattgaaacatattatatttgttttttttctatatttgtgtgtatatattgatttgtggttggattttaagGGCACAAGTATTGGGTTGGCCACCAATTCGATCTTATAGGAAGAACATTATGGCTAACCAGAAGAACAACAGTGACGAAATTAGTGACAGAGCTAGCAGTGGCAGTGGGGGTGCAGCGTTTGTGAAGGTTTGCATGGATGGTGCACCATATTTGCGTAAAGTAGATTTGAAGATGTACAACAGCTACCAAAAGTTATCCGATGCCTTGGCTAAGATGTTCAGTTCCTTCACCATGGgtaagttttacggttcgaaccttaacatgcagcttagggttgatacttcatcatcatggatagatataacatttgttttttaagacaatgatatgatattttgagggttgtaatagtaagaaatctgagtaacttggtgggtttttttttttttttgaagGGAGTTATGGATGTGAAGGAATGATAGACTTCATGAATGAGAGCAAGTTGATGGATCTTCTCAACAGTTCTGATTATGTGCCAACCTATGAAGATAAGGATGGCGACTGGATGCTCGTGGGCGATGTCCCATGGGAgtatgtttcacatattttatatatatatatatatgcatgctagtcttcattagatatctccccacagtcatgcatgcacatcttttagcagctcacctcacctacaaattaatctgctttcctttcgatatatattctagtacacccagttaggtgaaaaaattaacatttctagtatataattgagtttttattaatttattaaacgtaaaatgataaaaatattttcataattatataacttactttatacataaaagaatgttttaataatttctcagattaagttagtgtccgattgacacgtaacattaactcaattatgaacttaatataattataaatatagataaaagttgatgtacttatagtacgtaattctatgcacttaacatatctacatcaaaaaattttaaatctaaatctacttaacttcattttttaacaaaatctcatcattttatttcttattttttcacagGATGTTTGTTGAATCATGTAAACGTTTGCGCATAATGAAAGGATCAGAGGCAATAGGGCTTGgtacgtttgattttttatttatatacatacatgaattcaattcacaaatgtaaagttaatttgttgatattaatttcagCTCCAAGAGCAATGGAGAAATGCAAGAGCAGAGTCTAA-3’
序列6：
5’-ATGCTAGGAACTGAGCATGATTTGAACTTCAAAGAGACCGAGCTGTGTCTCGGCCTGCCTGGTGGAAGTGGTGGAGGAGGAGGAGCTGCCGCCGTCGGTGGTGGTGGTAAAAATGAAGTGGAAAGTTCCCCAAAGACCACCGGGAAAAGAGGCTACTCCGAGACTGTTGATTTGAAGCTCAATCTTCAGTCTAAAGAAGGTTGCATGGATCTGAATCAGAACCTAGAAAATGGTTCAAAAGAAAAGAATCATCTCCCTGCAAAACCACCAGCCAAGGCACAAGTATTGGGTTGGCCACCAATTCGATCTTATAGGAAGAACATTATGGCTAACCAGAAGAACAACAGTGACGAAATTAGTGACAGAGCTAGCAGTGGCAGTGGGGGTGCAGCGTTTGTGAAGGTTTGCATGGATGGTGCACCATATTTGCGTAAAGTAGATTTGAAGATGTACAACAGCTACCAAAAGTTATCCGATGCCTTGGCTAAGATGTTCAGTTCCTTCACCATGGGGAGTTATGGATGTGAAGGAATGATAGACTTCATGAATGAGAGCAAGTTGATGGATCTTCTCAACAGTTCTGATTATGTGCCAACCTATGAAGATAAGGATGGCGACTGGATGCTCGTGGGCGATGTCCCATGGGAGATGTTTGTTGAATCATGTAAACGTTTGCGCATAATGAAAGGATCAGAGGCAATAGGGCTTGCTCCAAGAGCAATGGAGAAATGCAAGAGCAGAGTCTAA-3’
以上所述仅为本发明的较佳实施例而已，并不用以限制本发明，凡在本发明的精神和原则之内所作的任何修改、等同替换和改进等，均应包含在本发明的保护范围之内。
Claims (2)
1.棉花GaIAA14基因在调控叶片形态中的应用，其特征在于，所述GaIAA14基因如序列5所示，所述调控为使棉花形成杯状叶。
2.如权利要求1所述的应用，其特征在于，GaIAA14基因CDS如序列6所示。
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202410671655.5A CN118272574B (zh) | 2024-05-28 | 2024-05-28 | 棉花GaIAA14基因突变位点及应用 |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202410671655.5A CN118272574B (zh) | 2024-05-28 | 2024-05-28 | 棉花GaIAA14基因突变位点及应用 |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN118272574A CN118272574A (zh) | 2024-07-02 |
| CN118272574B true CN118272574B (zh) | 2024-08-16 |
Family
ID=91638444
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202410671655.5A Active CN118272574B (zh) | 2024-05-28 | 2024-05-28 | 棉花GaIAA14基因突变位点及应用 |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN118272574B (zh) |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112226533A (zh) * | 2020-11-24 | 2021-01-15 | 石河子大学 | 与棉花卷叶性状相关的分子标记及其应用 |
| CN115976045A (zh) * | 2022-10-13 | 2023-04-18 | 三亚中国农业科学院国家南繁研究院 | 一种棉花GaTFL1基因及其应用和鉴定方法 |
Family Cites Families (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US9062322B2 (en) * | 2008-09-24 | 2015-06-23 | Basf Plant Science Gmbh | Plants having enhanced yield-related traits and a method for making the same |
| US10604769B2 (en) * | 2015-11-02 | 2020-03-31 | Monsanto Technology Llc | Cotton transgenic event MON 88702 and methods for detection and uses thereof |
| CN109486832B (zh) * | 2018-12-29 | 2021-11-23 | 中国农业科学院棉花研究所 | 一种创建有限生长株型棉花的方法 |
-
2024
- 2024-05-28 CN CN202410671655.5A patent/CN118272574B/zh active Active
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112226533A (zh) * | 2020-11-24 | 2021-01-15 | 石河子大学 | 与棉花卷叶性状相关的分子标记及其应用 |
| CN115976045A (zh) * | 2022-10-13 | 2023-04-18 | 三亚中国农业科学院国家南繁研究院 | 一种棉花GaTFL1基因及其应用和鉴定方法 |
Also Published As
| Publication number | Publication date |
|---|---|
| CN118272574A (zh) | 2024-07-02 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN111511920A (zh) | 与大豆中疾病抗性相关联的新颖的抗性基因 | |
| AU2016318051B2 (en) | Diplospory gene | |
| CA3175033A1 (en) | Autoflowering markers | |
| JP7659547B2 (ja) | 植物における還元糖含有量の調節（ｉｎｖ） | |
| KR20200136921A (ko) | 식물 내 아미노산 함량 조절 | |
| CN115125327A (zh) | 一种与玉米穗长相关的分子标记及其应用 | |
| CN115927381B (zh) | 一种油菜rna加工因子ncbp基因及其应用 | |
| CN116218876A (zh) | 一种调控水稻垩白的基因OsB12D3及其编码蛋白和应用 | |
| CN109912702A (zh) | 蛋白质OsARE1在调控植物抗低氮性中的应用 | |
| WO2022117024A1 (zh) | 孤雌生殖单倍体诱导基因及其应用 | |
| CN119120486B (zh) | 水稻基因sll4在调控水稻叶形中的应用 | |
| CN118272574B (zh) | 棉花GaIAA14基因突变位点及应用 | |
| CN103215289B (zh) | 导致西瓜两性花发育的基因a序列及其获得方法 | |
| CN118546947A (zh) | 一种温度响应的水稻垩白基因pgwc3及其编码蛋白和应用 | |
| CN118599895A (zh) | 一种调控水稻垩白的LOC_Os11g10170基因及其编码蛋白和应用 | |
| CN116814644B (zh) | 一个控制水稻分蘖数和花期的基因OsMADS22及其应用 | |
| CN103757018B (zh) | 与大白菜PHYB基因mRNA5′非翻译区突变相关的InDel标记及其应用 | |
| CN108239675B (zh) | 一种用于鉴定甜瓜单性花的分子标记物TJcM02及其应用 | |
| CN111073896A (zh) | 控制玉米籽粒灌浆的基因及编码产物、引物、载体和应用 | |
| CN117417957A (zh) | 一种增加水稻香味的方法 | |
| CN108531636B (zh) | 一种用于鉴定甜瓜单性花的分子标记物TJcM01及其应用 | |
| CN111172171A (zh) | 控制玉米株高和花期的基因及其应用 | |
| CN110724694B (zh) | 一种水稻育性基因saw1及其应用 | |
| CN116694799A (zh) | 水稻OsAUX5基因中与稻米必需氨基酸积累相关InDel的位点及应用 | |
| CN111518181B (zh) | 提高水稻产量的方法及其所用蛋白质 |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |