CN118203539A - Plant moisturizing composition and preparation method and application thereof - Google Patents
Plant moisturizing composition and preparation method and application thereof Download PDFInfo
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- CN118203539A CN118203539A CN202410276021.XA CN202410276021A CN118203539A CN 118203539 A CN118203539 A CN 118203539A CN 202410276021 A CN202410276021 A CN 202410276021A CN 118203539 A CN118203539 A CN 118203539A
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Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
- A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
- A61K8/9783—Angiosperms [Magnoliophyta]
- A61K8/9794—Liliopsida [monocotyledons]
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
- A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
- A61K8/9783—Angiosperms [Magnoliophyta]
- A61K8/9789—Magnoliopsida [dicotyledons]
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/40—Chemical, physico-chemical or functional or structural properties of particular ingredients
- A61K2800/59—Mixtures
- A61K2800/592—Mixtures of compounds complementing their respective functions
- A61K2800/5922—At least two compounds being classified in the same subclass of A61K8/18
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Veterinary Medicine (AREA)
- Animal Behavior & Ethology (AREA)
- General Health & Medical Sciences (AREA)
- Public Health (AREA)
- Engineering & Computer Science (AREA)
- Biotechnology (AREA)
- Botany (AREA)
- Microbiology (AREA)
- Mycology (AREA)
- Birds (AREA)
- Epidemiology (AREA)
- Dermatology (AREA)
- Cosmetics (AREA)
Abstract
The invention discloses a plant moisturizing composition, a preparation method and application thereof, and relates to the technical field of cosmetics. The invention provides a plant moisturizing composition which comprises the following components in parts by weight: 10-20 parts of sea buckthorn, 5-15 parts of tremella, 5-15 parts of corn silk and 2-10 parts of lophatherum gracile. The four plants of the invention are all medicinal and edible traditional Chinese medicines, have mild taste, accord with the current theory of 'homology of make-up and food', and do not cause burden to skin. The four plants of sea buckthorn, tremella, corn silk and lophatherum gracile are combined together for use, and certain effects are exerted in the formula respectively, so that the four plants are indispensable.
Description
Technical Field
The invention relates to the technical field of cosmetics, in particular to a plant moisturizing composition and a preparation method and application thereof.
Background
Moisturizing is the most basic step in skin care, and when skin is at a higher moisture content, the cells in the stratum corneum are closely and orderly arranged, with an intact skin barrier; on the contrary, when the moisture content of skin is lost, the skin becomes dry, and the barrier function is easily destroyed, so that the conditions of accelerated aging, fine lines, large pores and the like occur. In addition, moisture preservation plays a vital role in the exertion of functional products such as whitening and anti-aging, and is a basis and a key for relieving various skin problems.
Natural plant skin care, which is a concept of pursuing safe and comfortable skin care experience, becomes a more and more touted skin care trend in the years. The plant cosmetic has the characteristics of mild effect, small toxic and side effects, strong pertinence, lasting effect and the like, wherein the plant active ingredients are key components for playing the main roles of the plant cosmetic. The polysaccharide is an active polysaccharide extracted from natural plants, and has pharmacological effects in regulating immunity, delaying aging, resisting tumor and virus, etc. Meanwhile, the polysaccharide is used as a polymer carbohydrate polymer with various physiological activities and special health care functions, the structure of the polymer carbohydrate polymer contains a large number of hydroxyl groups, the polymer carbohydrate polymer can be combined with water in a hydrogen bond mode, meanwhile, the polymer carbohydrate polymer has good film forming property, and a water-locking film can be formed on the surface of the skin to prevent the water loss of the skin, so that the effect of lasting moisture preservation is achieved.
Many products currently claimed to moisturize only temporarily increase the moisture content of the stratum corneum, and treat the symptoms without the root cause. Therefore, the development of a plant moisturizing composition capable of comprehensively exerting moisturizing, preventing water loss from inside to outside and supplementing nutrition from outside to inside has very important practical value.
Disclosure of Invention
Based on the above, the present invention aims to overcome the above-mentioned shortcomings of the prior art and provide a plant moisturizing composition capable of preventing water loss from inside to outside and supplementing nutrition from outside to inside, and performing moisturizing in all directions, and a preparation method and application thereof.
In order to achieve the above purpose, the technical scheme adopted by the invention is as follows: the plant moisturizing composition comprises the following components in parts by weight: 10-20 parts of sea buckthorn, 5-15 parts of tremella, 5-15 parts of corn silk and 2-10 parts of lophatherum gracile.
White fungus: the tremella polysaccharide is a fruiting body of tremella fungus of basidiomycota, is taken as a traditional edible fungus in China, is a good health care product with homology of medicine and food, and contains tremella polysaccharide as an active ingredient, and has the effects of delaying aging, resisting radiation, enhancing immunity and the like. Sea buckthorn: is fruit of Hippophae of Elaeagnaceae, belongs to medicinal and edible plant, is rich in vitamins, saccharides, proteins, flavone and phenolic compounds, and has antioxidant, antiaging and immunity regulating effects. Corn silk: is a natural health care product with the homology of medicine and food, which is prepared from the columella and the stigma of the maize of the Gramineae family, contains abundant trace components, minerals, vitamins and the like, contains active components such as polysaccharide, flavone, sterol, organic acid and the like, and has the effects of resisting bacteria, diminishing inflammation, resisting oxidation and the like. Lophatherum gracile: the plant is dried stem and leaf of Lophatherum gracile of Gramineae, is a medicinal and edible plant, mainly contains flavonoid, triterpene, polysaccharide, etc., and has antibacterial, antiinflammatory, antioxidant, and antiaging effects.
The inventor finds that the 4 plants of sea buckthorn, tremella, corn silk and lophatherum gracile are combined together in the practical experimental process, and each of the 4 plants plays a certain role in the formula, which is indispensable. Tremella, which is called as having the effects of beautifying and tendering skin, has flat nature and sweet and light taste, is used as a monarch drug in the prescription, and the tremella polysaccharide which is rich in tremella polysaccharide can form a layer of protective film on the surface of skin to lock the moisture of the skin; sea buckthorn has sour and sweet nature and slight cold, can moisten lung and promote salivation, clear heat and detoxify, nourish yin and moisten dryness, and the like, and is used as ministerial drug in the prescription to play a role in moisturizing in cooperation with tremella; lophatherum gracile is sweet in nature, light in taste, cold in nature, capable of returning to heart, stomach and small intestine channels, has the effects of clearing heart fire, relieving restlessness, promoting urination and treating stranguria, is rich in various active ingredients and natural moisturizing factors, and is used as an adjuvant in the formula; corn silk, sweet and bland in flavor, neutral in nature, can regulate water passage and regulate all herbs as guiding drugs.
Preferably, the weight ratio of the corn silk and the lophatherum gracile= (1-3): 1. further preferably, the weight ratio of the corn silk and the lophatherum gracile=1: 1.
The inventor finds that under the condition that the monarch and minister formula is determined in the practical experiment process, the proportion of the two medicines has great influence on the moisturizing effect of the final moisturizing composition, and the reason is probably that the polysaccharide type and the structure in corn silk and lophatherum gracile can cooperate with sea buckthorn and tremella under the proportion, so that the better moisturizing effect is exerted.
In addition, the invention provides a preparation method of the plant moisturizing composition, which comprises the following steps:
(1) Pretreating sea buckthorn, tremella, corn silk and lophatherum gracile, adding water for reflux extraction, filtering, and concentrating under reduced pressure to obtain a concentrated solution;
(2) And adding ethanol into the concentrated solution, refrigerating, filtering/centrifuging, and drying to obtain the plant moisturizing composition.
Preferably, in the step (1), the ratio of the feed to the liquid after adding water is 1: (25-35), reflux-extracting for 1.5-2.5h; in the step (2), the alcohol concentration is 75-85% after ethanol is added, the refrigerating temperature is 2-6 ℃, and the refrigerating time is 10-14h.
Further preferably, in the step (1), the ratio of the feed to the liquid after adding water is 1:30, reflux extraction time is 2 hours; in the step (2), the alcohol concentration is 80% after the ethanol is added, the refrigerating temperature is 4 ℃, and the refrigerating time is 12 hours.
The inventor finds that the refrigerating temperature is 2-6 ℃, the refrigerating time is 10-14h, the precipitation effect of the alcohol precipitation polysaccharide is good, and the precipitation effect of the alcohol precipitation polysaccharide can be influenced under the condition of too high or too low temperature.
And when the refrigerating temperature is 4 ℃, the precipitation effect of the alcohol precipitation polysaccharide is optimal. In general, an excessively high temperature may damage the spatial structure of the alcohol precipitation polysaccharide, affect the precipitation effect thereof, and an excessively low temperature may affect the solubility of the alcohol precipitation polysaccharide, and also affect the precipitation effect thereof. The precipitation temperature is 4 ℃, and the precipitation effect of the alcohol precipitation polysaccharide is optimal.
Further, the invention provides application of the plant moisturizing composition in preparing cosmetics.
The invention provides a cosmetic, which comprises the plant moisturizing composition.
The invention provides a moisturizing essence, which comprises the plant moisturizing composition, wherein the weight percentage of the plant moisturizing composition in the moisturizing essence is 3-10%.
The invention provides a moisturizing facial cream which comprises the plant moisturizing composition, wherein the weight percentage of the plant moisturizing composition in moisturizing fine flowers is 3-10%.
Compared with the prior art, the invention has the beneficial effects that:
1. The four plants of the invention are all medicinal and edible traditional Chinese medicines, have mild taste, accord with the current theory of 'homology of make-up and food', and do not cause burden to skin. The four plants of sea buckthorn, tremella, corn silk and lophatherum gracile are combined together for use, and certain effects are exerted in the formula respectively, so that the four plants are indispensable.
2. The plant moisturizing composition prepared by the invention has a macromolecular structure, can form a water locking film on the surface of skin to prevent the outward loss of skin moisture, and simultaneously small molecular substances can permeate the skin to have the effects of resisting oxidation, delaying aging and the like, so that the skin problem is improved from inside, and the moisturizing advantage is obvious.
Drawings
FIG. 1 is a standard graph of glucose content measured by the phenol-sulfuric acid method;
fig. 2 is a graph showing the change of tail area before and after zebra fish treatment.
Detailed Description
For a better description of the objects, technical solutions and advantages of the present invention, the present invention will be further described with reference to the accompanying drawings and specific embodiments. In the examples, the experimental methods used are conventional methods unless otherwise specified, and the materials, reagents, etc. used, unless otherwise specified, are commercially available.
The following description of the raw materials used in the examples and comparative examples is provided, but is not limited to these materials: sea buckthorn, tremella, corn silk, lophatherum gracile, mesona chinensis, liquorice, raspberry and papaya are all purchased from Guangzhou Kangsheng medical company, and meet the medicinal standard of Chinese pharmacopoeia of 2020 edition. The following medicinal materials are used:
White fungus: fruiting bodies of Tremella fuciformis berk (Tremella fuciformis Berk.) belonging to Tremellaceae genus;
Sea buckthorn: dried mature fruit of Hippophae rhamnoides (Hippophae rhamnoides l.) belonging to the family Elaeagnaceae;
Lophatherum gracile: dried stem and leaf of Lophatherum gracile (Lophatherum gracile Brongn.) belonging to Gramineae;
Corn silk: a dried flower column of the gramineous plant maize (Zea mays l.);
mesona chinensis benth: whole grass of Mesona chinensis (Mesona chinensis benth.) belonging to genus Mesona of family Labiatae.
Licorice root: dried roots of Glycyrrhiza uralensis (Glycyrrhiza uralensis Fisch.) or Glycyrrhiza glabra (Glycyrrhiza inflata Bat.) or Glycyrrhiza glabra (Glycyrrhiza glabra L.) of Leguminosae.
Examples 1 to 4 and comparative examples 1 to 10
Example 1
The plant moisturizing composition provided by the embodiment of the invention comprises the following raw materials: sea buckthorn 10g, tremella 10g, corn silk 10g and lophatherum gracile 10g.
The preparation method of the plant moisturizing composition of the embodiment 1 comprises the following steps:
(1) Pretreating sea buckthorn, tremella, corn silk and lophatherum gracile, adding water for reflux extraction, filtering, and concentrating under reduced pressure to obtain a concentrated solution; the feed liquid ratio after adding water is 1:30, reflux extraction time is 2 hours;
(2) Adding ethanol into the concentrated solution, refrigerating, filtering/centrifuging, and drying to obtain the plant moisturizing composition; the alcohol concentration is 80% after adding ethanol, the refrigerating temperature is 4 ℃, and the refrigerating time is 12h.
Example 2
The only difference between this example and example 1 is that the plant moisturizing composition of this example is as follows: 12g of sea buckthorn, 8g of tremella, 8g of corn silk and 12g of lophatherum gracile.
Example 3
The only difference between this example and example 1 is that the plant moisturizing composition of this example is as follows: 16g of sea buckthorn, 14g of tremella, 6g of corn silk and 4g of lophatherum gracile.
Example 4
The only difference between this example and example 1 is that the plant moisturizing composition of this example is as follows: sea buckthorn 18g, tremella 6g, corn silk 12g and lophatherum gracile 4g.
Comparative example 1
The only difference between this comparative example and example 1 is that the plant moisturizing composition raw materials are as follows: sea buckthorn 30g.
Comparative example 2
The only difference between this comparative example and example 1 is that the plant moisturizing composition raw materials are as follows: 30g of tremella.
Comparative example 3
The only difference between this comparative example and example 1 is that the plant moisturizing composition raw materials are as follows: 30g of corn silk.
Comparative example 4
The only difference between this comparative example and example 1 is that the plant moisturizing composition raw materials are as follows: 30g of lophatherum gracile.
Comparative example 5
The only difference between this comparative example and example 1 is that the plant moisturizing composition raw materials are as follows: sea buckthorn 10g, tremella 10g and corn silk 10g.
Comparative example 6
The only difference between this comparative example and example 1 is that the plant moisturizing composition raw materials are as follows: sea buckthorn 10g, tremella 10g and lophatherum gracile 10g.
Comparative example 7
The only difference between this comparative example and example 1 is that the plant moisturizing composition raw materials are as follows: 10g of tremella, 10g of corn silk and 10g of lophatherum gracile.
Comparative example 8
The only difference between this comparative example and example 1 is that the plant moisturizing composition raw materials are as follows: sea buckthorn 10g, corn silk 10g and lophatherum gracile 10g.
Comparative example 9
The only difference between this comparative example and example 1 is that the plant moisturizing composition raw materials are as follows: sea buckthorn 10g, tremella 10g, corn silk 10g and mesona chinensis 10g.
Comparative example 10
The only difference between this comparative example and example 1 is that the plant moisturizing composition raw materials are as follows: sea buckthorn 10g, tremella 10g, lophatherum gracile 10g and liquorice 10g.
Application example 1
The moisture-preserving essence comprises the following components in percentage by weight: water: the balance, butanediol: 8%, glycerol polyether-26: 3%, 1, 2-hexanediol: 0.5%, PEG-40 hydrogenated castor oil: 0.02%, disodium EDTA: 0.05%, phenoxyethanol: 0.2%, yeast extract: 0.15%, octanoyl hydroxamic acid 0.2%, xanthan gum: 0.15%, citric acid: 0.04%, ethylhexyl glycerol: 0.1% of the moisturizing composition prepared in example 1: 5%.
Application example 2
Moisturizing facial cream, water: the balance, chlorophenyl glyoxyl: 0.15%, hydrogenated lecithin: 0.2%, palmitic acid: 0.3%, cetostearyl alcohol: 2%, glycerol: 2%, citric acid glyceride: 1%, almond oil: 5.5%, tocopherol: 0.1% of polydimethylsiloxane: 3%, phenoxyethanol: 0.6%, beeswax: 3.5% of the moisturizing composition prepared in example 1: 5%.
Effect verification
Test example 1 polysaccharide content determination
The test process comprises the following steps: and dissolving the extracts with the same quality in water, and measuring the polysaccharide content. Glucose is used as standard solution, and a phenol-concentrated sulfuric acid method is adopted. And drawing a standard curve by taking the glucose concentration (mug/mL) as an abscissa and the absorbance value (A) as an ordinate to obtain a regression equation.
Polysaccharide content (%) = (measured concentration x dilution x reaction liquid volume/mass of sample) ×100%. The content of polysaccharide is measured by phenol-sulfuric acid method, firstly, preparing a series of concentration solutions by using standard glucose as a standard curve, and specifically comprises the following steps: dissolving and precisely weighing 10.00mg of D-glucose with ultrapure water, transferring to a 100.00mL volumetric flask, adding ultrapure water to the scale, and shaking to obtain 0.1mg/mL glucose solution; and precisely transferring 0.0,0.2,0.4,0.6,0.8,1.0,1.2mL 0.1mg/mL of D-glucose solution into a 25mL colorimetric tube with a plug, respectively adding 0.5mL of 5% phenol, shaking uniformly, rapidly adding 2.0mL of concentrated sulfuric acid, sealing, shaking uniformly, carrying out boiling water bath for 20min, taking out, cooling to room temperature, measuring the absorbance value at 490nm, and preparing a standard curve chart 1 according to the absorbance value and the concentration. The polysaccharide content of each extract solution was measured in the same manner as in the standard curve preparation.
Test results: as shown in table 1.
TABLE 1
Test example 2 moisture retention test
The test process comprises the following steps: the extracts, glycerol and betaine are respectively weighed and prepared into solutions with the mass fraction of 5% by deionized water, about 2g of the solutions are respectively weighed and placed on a constant-weight glassware, the glassware is placed in a constant-temperature and constant-humidity box with the condition of 25 ℃ and 60%, the water content before being placed is designated as M 0, the water content after being placed for a plurality of hours is designated as M n, and the moisture retention of the sample is calculated according to the moisture retention=M n/M0 ×100%. The higher the water loss rate, the worse the water retention of the humectant. The smaller the water loss rate is, the stronger the water retention of the humectant is, and the better the long-term moisturizing effect is. The water loss rate is the change value in the table, the moisture retention rate is 100% at 0h, the moisture loss is caused with the increase of time, the moisture retention rate is reduced, and the water loss rate is the water loss rate when the water loss rate is different from the moisture retention rate at 0 h.
Water loss = 100% -12h moisture retention.
Test results: as shown in table 2.
TABLE 2
From table 2, the moisturizing effect of each example was compared with that of the conventional moisturizing agent glycerin and betaine by a weighing method. The results demonstrate that example 1 has better moisturizing effect.
The moisture-preserving effect measured by a specific gravity method can be seen that the content of the polysaccharide is not necessarily in direct proportion to the moisture-preserving effect, so that the increase of the content of the polysaccharide in a combination of the compositions is not necessarily the optimal solution for achieving the moisture-preserving effect, and other plants are matched to cooperatively play the moisture-preserving effect. Under the common cooperation of the specific sea buckthorn, tremella, corn silk and lophatherum gracile, the moisturizing effect is excellent, the polysaccharide extracted from different plants is different in structure, and the primary structure, the higher structure, the substituent, the molecular mass and other structural characteristics of the polysaccharide extracted from different plants can influence the activity of the polysaccharide.
Test example 3 Zebra fish moisturizing experiment
The test process comprises the following steps: the experiment of moisturizing zebra fish in vivo is verified by the example and glycerin and betaine. Wild zebra fish larvae of 72hpf (72 hours after fertilization) which develop normally are randomly selected to lie on a glass slide and photographed under a split microscope. The zebra fish embryos are then transferred to 96-well cell culture plates, 1 tail/well, corresponding solution is added to each well, a zebra fish dehydration model is established by using 2% concentration NaCl solution, and the zebra fish embryos are placed in a constant temperature incubator at 28 ℃ for 15min. After 15min, zebra fish were placed on a slide to lie flat and photographed under a microscope.
Specifically, the blank group: 200 μl embryo culture broth; model group: 100 μl embryo culture solution+100 μl 4% aqueous sodium chloride solution;
Example 1:100 μl of 0.2% composition 1 embryo culture solution+100 μl of 4% aqueous sodium chloride solution;
example 2:100 μl of 0.2% composition 2 embryo culture solution+100 μl of 4% aqueous sodium chloride solution;
example 3:100 μl of 0.2% composition 3 embryo culture solution+100 μl of 4% aqueous sodium chloride solution;
example 4:100 μl of 0.2% composition 4 embryo culture solution+100 μl of 4% aqueous sodium chloride solution;
Glycerol: 100 μl of 0.2% glycerol embryo culture solution+100 μl of 4% sodium chloride aqueous solution;
Betaine: 100 μl of 0.2% betaine embryo culture solution+100 μl of 4% sodium chloride aqueous solution;
The zebra fish tail area was measured with Image J and the tail area change rate (%) was calculated as follows: tail area change rate (%) = (post-dewatering area-pre-dewatering area)/pre-dewatering area×100%. The more positive the tail change area rate is, the better the moisturizing effect on the zebra fish is.
The humectant is reused on the basis of model building, and whether the humectant can improve the water retention capacity of the zebra fish under the condition that NaCl dehydrates the zebra fish is examined, so that the result is compared with a model group, and the improvement condition is obvious from the result.
Test results: as shown in table 3.
TABLE 3 Table 3
Group of | Tail area change rate (%) |
Blank control group | -0.67 |
Model group | -10.73 |
Example 1 | -0.31 |
Example 2 | -0.47 |
Example 3 | -0.35 |
Example 4 | -0.32 |
Glycerol | -0.73 |
Betaine (betaine) | -0.93 |
The moisturizing effect is necessary for cosmetics, the polyalcohol is a moisturizing agent commonly used for cosmetics, such as glycerin and is used as a traditional moisturizing agent, the moisturizing effect is good, and compared with the traditional moisturizing agent glycerin and betaine through Ma Banma moisturizing experiments, the moisturizing effect is verified to be good.
As can be seen from the results of the zebra fish moisturizing experiments in table 3 and fig. 2, the composition can achieve the effect similar to that of the glycerin and betaine moisturizing agent, which indicates that the plant composition can be used as a whole for replacing the glycerin, betaine and other moisturizing agents, and can achieve the effect similar to that of the glycerin and betaine moisturizing agent and other effects of the plant.
Test example 4 evaluation of moisturizing efficacy of human body
The test process comprises the following steps: the skin stratum corneum moisture content was measured on a 3cm×3cm mark area on the inner side of the forearm of a subject using a skin multi-probe tester Courage & Khazaka MPA10, the higher the stratum corneum moisture content indicated the higher the skin water storage, the better the moisturizing effect and the more perfect the skin barrier function.
Subject basic information
Screening 31 qualified subjects (7 men and 24 women) mainly with mixed and dry skin, wherein 0 subjects fall off, and 31 subjects finish trial tests for 2 hours according to requirements, wherein the ages are 30-47 years, the average age is 38.6+/-4.5 years, and the effective rate is 100%.
Test flow
31 Panelists were screened, the arms were cleaned after visit, the areas where samples and controls were used were specified, the basal values were tested, and the blank control areas were smeared with purified water and tested 2 hours after product trial.
(1) Before use: screening-check-in, informed consent-cleaning of forearm-labeled area (3 cm x 3cm, 1cm apart per area) -defining areas for sample and control use-sitting for 20 minutes-testing basal values of skin stratum corneum moisture content of labeled area;
(2) And (3) sample coating: weighing and coating samples by workers, and recording the sample coating quantity and time; post-use index test: after 2 hours of use of the sample, the marked area was tested for skin stratum corneum moisture content.
(3) Data analysis: the data analysis used SPSS21.0 statistical software. The SW method was used to check the significance of the normal distribution of data prior to statistics. Sig (double sided) >0.05 then normal distribution, otherwise non normal distribution. The normal distribution data adopts a paired sample T test analysis method to carry out differential analysis on the baseline value (T0: before using the product) and the return visit value (T2 h) of each parameter, the non-normal distribution is applied to Wilcoxon rank sum test, and the statistical significance difference is marked by p < 0.05.
And comparing the sample area with the blank control area, wherein normal distribution data adopts T test to compare the change amount (T2 h-T0 h) of each parameter of the sample area and the blank control area, non-normal distribution adopts Wilcoxon rank sum test to compare the change amount (T2 h-T0 h) of each parameter of the sample area and the blank control area, differential analysis is carried out, and statistical significance difference is marked by p < 0.05. The parameters of the sample area are better than those before the use or the change amount is better than that of the blank control area, and the parameters have statistical significance, so that the sample has corresponding effects.
Test results: as shown in table 4.
TABLE 4 Table 4
Saccharide isomerate is produced by converting edible corn saccharide into a saccharide complex similar to the carbohydrate complex (NMF) found in human skin stratum corneum. It can combine with epsilon-amino group of lysine in keratinocyte keratin, and this unique binding mechanism makes it difficult to wash away as moisturizing active ingredient, and continuously improves skin hydration. The moisturizing effect of the four plants can be improved by carrying out disassembly and extraction on the formula and experimental evaluation, so that the effect is better than that of saccharide isomers when the four plants are used together, and the four plants respectively exert certain effect in the formula.
Finally, it should be noted that the above embodiments are only for illustrating the technical solution of the present invention and not for limiting the scope of the present invention, and although the present invention has been described in detail with reference to the preferred embodiments, it should be understood by those skilled in the art that the technical solution of the present invention may be modified or substituted equally without departing from the spirit and scope of the technical solution of the present invention.
Claims (9)
1. The plant moisturizing composition is characterized by comprising the following components in parts by weight: 10-20 parts of sea buckthorn, 5-15 parts of tremella, 5-15 parts of corn silk and 2-10 parts of lophatherum gracile.
2. The plant moisturizing composition according to claim 1, wherein the weight ratio of corn silk and lophatherum gracile is 1 = (1-3).
3. The plant moisturizing composition of claim 1, wherein the weight ratio of corn silk to lophatherum gracile = 1:1.
4. A method for preparing the plant moisturizing composition according to any one of claims 1 to 3, comprising the steps of:
(1) Pretreating sea buckthorn, tremella, corn silk and lophatherum gracile, adding water for reflux extraction, filtering, and concentrating under reduced pressure to obtain a concentrated solution;
(2) And adding ethanol into the concentrated solution, refrigerating, filtering/centrifuging, and drying to obtain the plant moisturizing composition.
5. The method of producing a plant moisturizing composition according to claim 4, wherein in the step (1), the ratio of the feed liquid after adding water is 1: (25-35), reflux-extracting for 1.5-2.5h; in the step (2), the alcohol concentration is 75-85% after ethanol is added, the refrigerating temperature is 2-6 ℃, and the refrigerating time is 10-14h.
6. Use of a plant moisturizing composition as claimed in any one of claims 1 to 3 for the preparation of cosmetics.
7. A cosmetic comprising the plant moisturizing composition of any one of claims 1 to 3.
8. A moisturizing concentrate comprising the plant moisturizing composition of any one of claims 1-3, wherein the plant moisturizing composition comprises 3-10% by weight of the moisturizing concentrate.
9. A moisturizing facial cream comprising the plant moisturizing composition of any one of claims 1 to 3, wherein the plant moisturizing composition comprises 3 to 10% by weight of the moisturizing facial cream.
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