KR20110128385A - Herbal Composition and Preparation Method for Acne Improvement Using Madam Cleansing - Google Patents

Abstract

The present invention is a red-green ginseng, baekbokyeong, raw jihwang, honey, baekmun-dong, astronomical dong, phalanges, sulfur, turmeric, rhubarb, golden, gardenia and wormwood containing natural herbal-derived complex herbal extracts (MiDam Cheong GyeolAn, MDCGA) as an active ingredient ), the present invention relates to antibiotic of acne containing as an active ingredient, reproduction, whitening improvement and herbal cosmetic herbal composition, not midam cleanliness of the present invention (MiDamCheongGyeolAn, MDCGA) is the antioxidant of the antimicrobial, skin cells for Propionibacterium acnes, cell physiology It was invented for the purpose of developing a composition for promoting activity and improving skin regeneration and whitening.

1

Description

The herb medicine composition material and the manufacturing process for pimple skin-improvement uses by Midam CheongGyeolAn}

The present invention relates to a composition made using undamaged cleansers, and more particularly, to a composition for cosmetics, a composition for soap, and a method for producing the same.

The exact cause of acne is not known, but the causes of acne include increased sebum secretion, inflammation and inflammation caused by androgen, a hormone that stimulates sebaceous glands, and propionibacterium acnes , which develops and breaks down sebum and forms free fatty acids. It is known that it is caused by a combination of major factors such as abnormal keratinization and genetic predisposition. Aerobic skin flora is not the primary cause of inflammation, but has been shown to further expand the site of inflammation, affecting acne worsening. Treatment includes the use of oral antibiotics and retinoids, along with the application of external medications, scrubs, and chemical peels. In particular, inflammatory acne produced by bacterial infection can improve symptoms through antibiotic treatment, but recently antibiotics such as tetracycline, clindamycin, and erythromycin, which are mainly used to suppress P. acnes proliferation and reduce inflammatory response, have been used for a long time. This can cause side effects or tolerate the treatment, making it less effective. In addition, the combination of retinoids with potent solubilizing function and antibiotics for the purpose of inhibiting P. acnes proliferation may be effective in inhibiting acne formation.

A jadeite is a kind of drowning medicine that helps life and prevent blood, and according to consent, it has a great effect on maintaining and activating the functions of all organs in the human body. In addition, the jadeite is not only a medicinal agent and a medicinal agent, but in particular, the intestine has a weak stomach and fatigue, the stomach is weak and the indigestion is severe. It is said to be effective for patients who catch cold, breathe with bronchial asthma and have severe coughing of seawater. Especially, when the weak and weak are worn by the elderly, the gray hair is black, the teeth are newly sprouted and the life is extended. .

        On the other hand, according to the results of modern research on jadeite, jadeite has an antioxidant effect, which contributes to cell physiological activity and regeneration. In addition, jadeite is effective for alleviating the skin aging phenomenon, it is known to improve acne skin condition and have a skin whitening effect.

In order to overcome the problems of acne treatments, there is a need for a safe natural-derived oriental medicine approach.

Panax ginseng CAMeyer roots include ginseng saponins (ginsenoside Ra, Rb1, Rb2, Rb3, Rc, Rd, Re, Ro) having various physiological activities. It also contains many types of amino acids, sugars, vatamin, flavonoids, volatile essential oils and minerals. Ginseng has the effects of weakness and weakness due to lack of energy, weakness of memory, boredom, fatigue, sweaty symptoms and mental relaxation, and excellent effects on mental strength, thinking power, and erectile dysfunction. It is used as an anti-diabetic and analgesic medication. The pharmacological action of ginseng includes hypoglycemic action, anticancer effect, serum protein synthesis promotion, antibody production promotion action, central excitement action, anti-fatigue action. In addition, it is effective for psychogenic shock, and has a significant anticancer effect in patients with gastric cancer surgery in the middle and end stages. It is effective (Illustration of Herbal Medicine, Ilseok Seo, Shin Mingyo, Books Yeonglimsa, p. 440-441, 1998). Ginseng referred to herein includes red ginseng.

Poria cocos Wolf contains β-pachyman in the nucleus, accounting for about 93% of dry weight. It also contains pachymic acid, tumulosic acid, 3 β-hydroxylanosta-7, 9 (11), and 24-triene-2-acid of triterpeniod compounds. In addition, it contains plant rubber, chitin, protein, fat, sterol, lecithin, glucose, adenine, histidine, choline, β-pachyman degrading enzyme, lipase and proteinase. Efficacy is diuretic, antibacterial, digestive system acts to prevent ulcers and gastric acid. It also acts to lower blood sugar (a diagram on drug metabolism, information stone, edited by Shin Min-gyo, book published by Young Lim, p. 41-42, 1998).

Rehmannia glutinosa is a perennial herb of Hyunsam. The height is about 30cm, the leaves are gathered and long oval. In June-July, purple flowers bloom in total inflorescences, and the fruit bears an oval capsule. The root is medicinal and originated from China and distributed all over Korea. The main components of the rehmannia root are β-sitosterol and mannitol, and they contain small amounts of stigmasterol and trace amounts of campesterol, rehmanin, alkaloid, fatty acid, capalpol (catalpinoside, des-p- hydroxybenzolcatalpside), glucose and 0.0053% vitamin A group. It contains. The root also contains stachyose, 4.2% arginine and 3.0% γ-butyl amino acids. The taste is sweet and bitter and the nature is cold. Efficacy is clear, positive blood, jinjin, jigal. Heat alteration, consciousness, confusion, inconspicuous, cures ring, hemorrhage, nasal bleeding, sea bleeding, sputum, constipation (Schematics of metabolic medicine, information stone, Shin Min-kyo, Book Publishing Younglimsa, p.907-908) , 1998).

Honey is a refined saccharide substance formed gradually from the traditional honey and beekeeping of the seal family. The fresh one is a translucent thick liquid which becomes discolored and opaque when stored for a long time and becomes white granular crystals when stored in a cold dark place. The taste is sweet, mild and nontoxic. It softens the lungs, controls the sea water, and exercises the intestines, which is effective for constipation. In addition, it has good detoxification effect when you are addicted to taking raw cucumbers or fresh cucumbers.

Baekmundong ( Liriope platyphyllla ) is the tuber of lobular vegetation and constant velocity plant. Most of the commodities are cultivated. It is usually excavated in early May of the second or third year after cultivation, and wild cans are harvested from early April. Cut only the root muscles, wash them clean, dry them in the sun for 3 to 4 days, and stack them in a well-ventilated place to evaporate the water. At this time, dry once every three days. Repeat this two or three times, remove the roots and miscellaneous goods. The taste is sweet and the nature is cold. Enter the lungs, stomach and heart. As a component, the pulmonary muscle of pulmonary sinus contains various steroid saponids and its aglycone is ruscogenin. It also contains β-sitosterol, stigmasterol, and β-sitosterol-β-d-glucoside. The fruit contains ophioside, which is kaempferol-3-glucogalactoside. Pharmacological action lowered blood sugar, and there was some antibacterial effect on whitepocoid bacillus and Escherichia coli (Depicted in Chinese medicine metabolism, information stone, Shin Mingyo, Yeonglimsa Publishing Co., p. 177-178, 1998).

The astronomical dong is the root of Asparagus cochinchinensis , and it is good to dig in the fall and winter and to be in the winter. After cleansing, remove the beard root and classify according to the size. Boil it with boiling water so that the skin is easily peeled off or steam it. Take off the skin when it is soaked in fresh water and wash it cleanly. Or fumigation with sulfur and redo again. The taste is closed, the nature is cold and nontoxic. Enter the lungs, nerves. The roots contain asparagine, mucus, β-sitosterol and 5-methoxymethyl-furfural, and the glutinous constituents are steroid saponin, consisting of smilagenin, rhamnose, xylose and glucose. Efficacy includes antimicrobial activity, mosquitoes, insecticidal action of fly larvae and anti-tumor effect (figure of medicine, metabolic warfare, information stone, Shin Mingyo, Book Publishing Yeonglimsa, p. 164-165, 1998).

Lycium chinense The root of the goji berry. After the spring and after harvest, collect the mud, shake off the mud, peel off the skin and dry it in the sun. The taste is sweet and the nature is cold. Enters the lungs, liver and nerves. Root skin contains cinnamon acid and a large amount of phenolic substance, betaine. Betaine is found in the leaves and fruits of the wolfberry tree, but has been reported to be absent in the root bark. The root contains other substances that inhibit the activity of vitamin B ₁ , but the inhibitory action is released by cystein and vitamin C. Phalanges include β-sosterosterol, linoleic acid, linolenic acid, and melissic acid. Pharmacological action acts on the cardiovascular system, and when intravenous injection is given to dogs, cats, and rabbits who have anesthesia and tincture of the phalanges, a pronounced coercive action appears and the heart rate decreases, resulting in faster breathing. There are also hypoglycemic and antipyretic effects (figure of medicinal herbs, information stone, edited by Shin Min-kyo, Young-lim Publishing Co., p. 827-828, 1998).

Sulfur is a natural sulfur ore of the sulfur family, a mineral of natural elements. It is mainly obtained by smelting of substances or minerals. Properties are acidic, toxic and act on the kidneys and colon. The pharmacological action of the external effects of the antagonism and insecticides, so apply to the symptoms of improvement, ungjo, etc. This is because of the action of poisonous poisoning. The drug is mild in nature, and is not suitable for the general nourishment except for special combinations (Clinical Herbology, Shin Mingyo, Book Publishing Younglim, p. 860-861, 2000).

Turmeric ( Curcuma longa L. ) is a rhizome rhizome , boiled or steamed in autumn and winter and dried in the sun. Beauty is reported, sex is warm and nontoxic. Rain enters the liver. Root stock contains 4.6-6% of essential oils. Essential oils include turmerone 58%, zingerene 25%, phellandrene 1%, 1, 8-cineole 1%, sabinene 0.6%, borneol 0.5%, dehydroturmerone, and more curcumin 0.3% and other arabinose 1.1%, fructose It contains 12% glucose, 28% milk fat, starch and oxalate. Efficacy first is to increase the bile secretion to restore to normal. Secondly, it acts on the uterus and the uterus causes paroxysmal contraction and lasts 5-7 hours. It has other cholesterol-lowering effects on blood and also has antimicrobial and hepatitis viruses (figure pill metabolism, information stone, Shin Min-kyo, book publication Young Lim, p.257-258, 1998).

Husband ( Coptis japonica Makino ) is a rhizome root, and November is the best season for harvesting. Dig out the poles to remove roots, leaves, mustache roots, mud, etc. and dry them in the sun to remove the cork layer. The taste is sweet and the nature is cold. Enter the heart, liver, stomach, and colon. As a component, rhubarb contains alkaloids such as berberine 7-9% coptisine, worenine, palmatine and jateorrhizine. In addition, it contains obacunone and obaculactone. Efficacy is first, antimicrobial and antipathogenic action. Secondly, it acts on the circulatory system and acts as a coercion. At normal doses and at low doses, the heart was excited and the coronary blood flow increased, but at high doses the heart was inhibited. Third, small doses of berberine in mammals act on acetylcholine, which enhances the action of acetylcholine and antagonizes it when administered in large quantities. The fourth acts on the smooth muscle of the blood vessels to relax, but the uterus, bladder, bronchial, gastrointestinal smooth muscles, such as excitement. Fifth, it has a function of enhancing gallbladder function, promotes the production of bile, and dilutes its concentration, so that patients with chronic cholecystitis have a high therapeutic effect. -493, 1998)

Mugwort ( Artemisa princeps Pampan ) is a dried leaf of yellow sea wormwood and related plants. In spring and summer, the leaves are lush, but when the flowers have not yet bloomed, they are collected and dried in the sun or dried in the shade. Taste is bitter and temper is mild. It enters the nose, liver and nerves. Yellow sea wormwood contains essential oils and the highest content of essential oils is cineole, which accounts for 25 ~ 30%. In addition, β-caryophyllene, terpinen-4-ol, linalool, artemisia, alcohol, camphor, bornol, etc. are contained. Leaves contain tetracosanol, β-sosterosterol, l-chebulachitol, and l-inositol. First of all, pharmacological action is antibacterial action, or when fumigation with irises and manures or mixed with manures, manures and white papers, fumigation, Staphylococcus aureus, β-hemolytic streptococci, Escherichia coli, Proteus, Diphtheria, Typhus and Paratyphus , Pseudomonas aeruginosa, Bacillus subtilis, Clostridium alcaligenes, Mycobacterium tuberculosis all have bactericidal or inhibitory effects. Second, shorten the bleeding and coagulation time and the peat action becomes more pronounced. The third small amount (3 ~ 5g) promotes secretion of gastric juice and appetite, and in large quantities causes acute inflammation of the stomach, resulting in nausea and vomiting. 1015, 1998).

Golden ( Scutellaria baicalensis Geogri .) Is a perennial herbaceous golden root of Lamiaceae. It acts on the lungs, bile, stomach and colon. Gold is bitter and cold, dehumidifying and removing heat. It mainly acts on the lungs and is used for cough, sputum due to the heat of the lungs. Antipyretic, anti-inflammatory, diuretic, gastric secretion, antibacterial action. Baikalin contained in gold (Baicalin) is a sedative effect and hemostasis by reducing the permeability of capillaries. Baikal is hydrolyzed by decomposing glucuronic acid (dilution) and diuretic baikallein (Baicalein). In addition, bicarin has an excellent antibacterial effect, which also inhibits diphtheria bacteria, pertussis bacteria, and influenza viruses (Jin Bo-seop and Shin Mingyo, Dohae Herbal Medicine (Medicinal Herbs)), Younglimsa, pp855-856, 1998.

Gardenia jasminoides are the flavonoids of gardenin, pectin, tannin, crocin, crocetin, d-mannitol, and nonaco. Glycosides of various types of iridoide bones, in addition to acid (nonacosane) and beta-sitosterol, such as gardenoside, geniposide (genipin-1-glucoside), and genipine-1 It contains -β-d-genithiobioside and a small amount of shanzhiside and is also used as a gardoside, scandoside methyl ester, choline (choline) and ursolic acid. It also has a calming, blood pressure-lowering effect, constricts the gallbladder, increases bile secretion, and has an biliary effect. It has hemostatic effect and hemostatic effect of raw gardener is stronger than that of beginners (Jung Ji-sup and Shin Min-kyo)

Madam cleansers are concentrates or extracts thereof which have been concentrated by bathing a combination of red ginseng, turmeric, fueryeong, buckwheat and sulfur, turmeric, yellow lotus, gold, gardenia and wormwood as described above. With the combination of the red ginseng, jihwang, bokyeong, abdominal wheat, sulfur, turmeric, yellow lotus, gold, gardenia and wormwood, undamaged clean water has a variety of pharmacological activity. In particular, it has an effective effect in antibacterial activity, cell activity and skin regeneration.

There is a lot of research on the development of antimicrobial agents derived from natural products, but there are not many cases that actually lead to the demonstration and commercialization of efficacy. In addition, in the above-mentioned documents and research reports so far, there is no teaching or disclosed about the skin improvement effect and the skin regeneration effect on acne of the herbal combination extract. Therefore, the present inventors have completed the present invention by confirming the skin improvement effect and improvement of skin regeneration against acne in the clear clear eye.

The technical problem to be achieved by the present invention is to provide a composition made by using a bland cleansing, improved skin regeneration, cell physiological activity or antimicrobial efficacy against acne. Another technical problem to be achieved by the present invention is to provide a method for producing the composition as described above.

The present invention is an acne skin improvement, cell activity, skin regeneration and containing complex herbal extracts including red ginseng, baekbokyeong, raw jihwang, honey, baekmun-dong, astronomical dong, phalanges, sulfur, turmeric, yellow lotus, golden, gardenia and wormwood as an active ingredient A cosmetic herbal composition is provided (hereinafter referred to as MiDam Cheong GyeolAn, MDCGA).

        MDCGA of the present invention is optimized by optimizing the red ginseng, baekbokyeong, raw jihwang, white wheat, baekmun-dong, astronomical dong, phalanges, sulfur, turmeric, rhubarb, gold, gardenia and wormwood at an appropriate ratio to improve acne skin and increase cell physiological activity It not only has the effect of skin regeneration, but also has a very good effect in terms of skin safety with respect to the existing sulfur component.

1 is a flow chart of a method for manufacturing MDCGA according to an embodiment of the present invention.
Figure 2 is the antibacterial activity of unbleached cleansing against acne causing bacteria. 100 µl of propionibacterium acnes (A) and Stapylococcus aureus (B) were smeared, and 50 µl of 1% (a) and 10% (b) of unbleached clear solution was incubated at 37 ° C for 12 to 24 hours. It is a clear zone where microorganisms do not grow around the paper disk of the medium.
Figure 3 is a photograph verifying the improvement effect of the acne skin of the skin (A) of the subject's face before and after use for two months before and after using the cosmetic composition containing unclear cleansing solution.

In order to achieve the above object, the present invention acne-inducing bacteria containing a complex herbal extract containing red ginseng, baekbokyeong, raw jihwang, white wheat, makmundong, astronomical dong, phalanges, sulfur, turmeric, yellow lotus, gold, gardenia and wormwood as an active ingredient Inhibition of the production and improvement of skin regeneration and provides a cosmetic herbal composition.

The composition ratio of each herbal composition of the present invention is not limited thereto, but preferably, the individual weight composition ratio of red ginseng, baekbokyeong, saengjihwang, white wheat, makmundong, astronomical dong, phalanges, sulfur, turmeric, rhubarb, golden, gardenia and wormwood is about 1 ~ 10: 1 ~ 10: 1 ~ 10: 1 ~ 10: 0.1 ~ 1: 0.1 ~ 1: 0.1 ~ 1: 1 ~ 5: 0.1 ~ 1: 0.1 ~ 1: 0.1 ~ 1: 0.1 ~ 1: 0.1 ~ 1 More preferably, about 1 to 5: 1 to 5: 1 to 10: 1 to 10: 0.1 to 0.5: 0.1 to 0.5: 0.1 to 0.5: 1 to 3: 0.1 to 0.3: 0.1 to 0.3: 0.1 to 0.3: 0.1 to 0.3: 0.1 to 0.3, most preferably 1 to 2: 1 to 2: 1 to 10: 1 to 6: 0.1 to 0.3: 0.1 to 0.3: 0.1 to 0.3: 1 to 2: 0.1 to 0.2: 0.1 to 0.2: 0.1-0.2: 0.1-0.2: 0.1-0.2.

Herbal medicine composition for the treatment and prevention of acne containing uncleaned compound combination herbal extract of the present invention, the total weight of the composition comprises 0.1 to 50% by weight of the extract.

Hereinafter, the present invention will be described in detail.

Looking at the manufacturing process of the undamaged cleansing solution of the present invention, red ginseng, baekbokyeong, saengjihwang, makmundong, cheonmundong and phalanges. The blending ratio may be red ginseng powder 3Kg, Bokyeong powder 6kg raw turmeric 18kg, white wheat 10kg, Mcmundong 900g, cheonmundong 900g, phalanx 900g, sulfur 2kg, turmeric 300g, sulfur lotus 300g, golden 300g, gardenia 300g and mugwort 300g. However, the compounding ratio is not limited to the examples listed above, of course, can be added or subtracted as necessary.

Subsequently, the formulation is primaryly bathed with the exception of sulfur, turmeric, yellow lotus, gold, gardenia and wormwood. The primary bath is put into the formulation evenly mixed in a closed pot, it may proceed to the bath treatment at a temperature of 95 ℃ for about 3 days. The formulation is then cooled. The cooling time may be for example about 1 day.

Again, the cooled mixture is secondary bathed. The secondary bath can be bathed at a temperature of 90 degrees Celsius for 24 hours to remove excess water.

The cooled mixture is then powdered and then finely powdered after addition of powders of sulfur, turmeric, rhubarb, gold, gardenia and wormwood, resulting in an unclear cleansing solution.

Herbal medicine Scientific name Volume (g) Red ginseng Panax ginseng C.A.Meyer 3000 Baikyeongryeong Poria cocos wolf 6000 Living place Rehmannia glutino 18000 White wheat Honey 10000 Macmundong Liriope platyphyllla 600 Astronomical building Asparagus cochinchinensis 600 Phalanges Lycium chinense 600 brimstone sulfur 2000 curcuma Curcuma longa L. 300 goldthread Coptis japonica Makino 300 Gold Artemisa princeps Pampan 300 Gardenia Scutellaria baicalensis Geogri . 300 Mugwort Gardenia jasminoides 300

Such unclean cleaner products may have a content of 0.0001 to 30% by weight relative to the total weight of the composition according to the embodiments of the present invention. Preferably the unsealed cleansing product may have a content of from 0.001 to 20% by weight relative to the total weight of the composition.

More specifically, the composition according to some embodiments of the present invention can be applied to the cosmetic composition using the effects of excellent acne antimicrobial, acne skin improvement, stability of unbleached cleansing. In this case, the composition may have any one formulation selected from the group consisting of lotion, essence, lotion, cream, pack, gel, ointment, patch, soap formulation and spray.

When the composition is applied as a cosmetic composition, the composition may further comprise a carrier that can be accommodated in the cosmetic, in addition to the above-mentioned non-clear cleansing. The cosmetically acceptable carrier may be included in cosmetic preparations, and includes those having a degree of toxicity, stability, and irritation requirements to which the human body is adapted to contact with the skin. Carriers acceptable for cosmetics may be, for example, alcohols, oils, surfactants, fatty acids, silicone oils, wetting agents, moisturizers, viscosity modifiers, emulsions, stabilizers, sunscreens, colorants, perfumes, and the like, and one or more cosmetic agents selected from these. Carriers acceptable to may be included in the composition, but are not limited thereto.

The content of the carrier acceptable in the above cosmetics relative to the total weight of the composition may be, for example, 1 to 99% by weight, preferably 90 to 99% by weight. However, the present invention is not limited thereto, and the content may vary depending on the formulation of the above-described composition or the site to which the composition is applied.

In addition, the composition according to another embodiment of the present invention can be applied as a pharmaceutical composition using the effects of antibacterial, acne skin improvement, stability of the excellent acne bacterium in unclear cleanliness. The undamaged cleansing solution included in the composition may further have pharmacological effects such as acne dermatitis improvement and skin trouble improvement.

When the composition is applied as a pharmaceutical composition it may have any one formulation selected from the group consisting of solutions, suspensions, emulsions, lotions, and ointments. Pharmaceutical compositions comprising unclear clear eyes may be administered to the human body in a parenteral manner. Preferably it may be administered directly to the skin in a topical manner. In this case, the dosage may be, for example, 0.001 to 150 mg / kg per day, and may be administered once or in several divided doses. However, the dosage is only one example and may be modified according to the route of administration, the age, sex, weight, severity, etc. of the patient.

Hereinafter, the present invention will be described in detail by Examples, Formulation Examples and Experimental Examples.

However, the following Examples, Formulation Examples and Experimental Examples are merely illustrative of the present invention, and the content of the present invention is not limited by the following Examples, Formulation Examples and Experimental Examples.

Preparation Example 1 Preparation of Undamaged Cleansing Solution

The herbal medicines listed in Table 1 purchased by the modern pharmacist are firstly bathed in the combination with the herbal medicines except sulfur, turmeric, yellow lotus, gold, gardenia and wormwood. The primary bath is put into the formulation evenly mixed in a closed pot, it may proceed to the bath treatment at a temperature of 95 ℃ for about 3 days. The formulation is then cooled. The cooling time may be for example about 1 day.

Again, the cooled mixture is secondary bathed. The secondary bath can be bathed at a temperature of 90 degrees Celsius for 24 hours to remove excess water. After cooling, it is powdered. The powder is finely powdered by adding powders of sulfur, turmeric, yellow lotus, gold, gardenia and wormwood. The result is an unclear clean solution.

Experimental Example 1. Measurement of antimicrobial activity of unbleached clear eyes by Agar diffusion method

The agar diffusion method was used to measure the antimicrobial activity of unbleached clean solution against acne and inflammatory bacteria. Actinomyces Broth solid medium capable of growing Propionbacterium acnes bacteria was prepared, and 100 µl of Propionbacterium acnes culture medium was plated on the intermediate stage of exponential growth. In addition, 100 μl of Stapylococcus aureus was plated on a Nutrient Broth solid medium for Stapylococcus aureus , which is in the middle stage of the exponential growth phase, which is an inflammation of the skin. After drying the surface of the medium for 10 minutes to each microbial culture medium, a 10 mm diameter paper disk was placed on the medium, and 50 μl of a solution of 10% and 1% (w / v) diluted uncleaned solution of Preparation Example 1 was applied. . After 12-24 hours of incubation at 37 ° C, the diameters of the clear zones in which microorganisms do not grow around the paper disk were checked to compare the antimicrobial activity.

As a result of verifying the antibacterial activity using acne bacteria Propionibacterium acnes (KCTC 3320) and skin inflammation bacteria Stapylococcus aureus (KCTC 1928) as shown in FIG.

The addition of 1% of uncleaned eye drops resulted in about 11 and 10 mm of stop ring in Propionibacterium acnes and Stapylococcus aureus , respectively. The addition of 10% undamaged cleansing solution increased the size of the low ring to Propionibacterium acnes and showed high antimicrobial activity of 21mm. In addition, low ring of about 15 mm of Stapylococcus aureus was observed. It was confirmed that undamaged cleansing solution had high antibacterial activity against acne causing bacteria.

Experimental Example 2. Growth Inhibition Effect of Undamaged Cleansing Solution against Acne Bacteria

In this experiment, the antimicrobial activity of Propionibacterium acnes and Stapylococcus aureus of undamaged cleanse was examined by agar diffusion method, and the antimicrobial activity was directly applied to the culture medium. To find out. After preparing a medium (1.0%, 0.5%, 0.1%, w / v) containing uncleaned solution of Preparation Example 1, Propionbacterium acnes [medium: Actinomyces Broth] and Stapylococcus aureus , a major bacterium causing inflammation on the skin, were prepared. Medium used: Nutrient Broth] was added to the liquid medium at 1 × 10 ⁵ to 1 × 10 ⁶ CFU / ml, and samples were sampled at regular intervals with shaking culture at 37 ° C., followed by appropriate dilution with distilled water, and OD at 600 nm. The growth of bacteria was confirmed by measuring the value.

Figure 2. Growth inhibition effect of propionibacterium acnes at various concentrations of unbleached clear eyes.

Figure 2 shows the OD values measured by time of day for the effect of uncleaned solution on the growth of Propionibacterium acnes . The extract of unbleached cleansed eye was inoculated to 1 × 10 ⁵ cfu / ml by adding P.acnes culture medium to 0.1% ~ 1.0% (w / v). In the medium containing no fresh cleansing extract, the induction period of the bacteria was 14 hours, after which the log enters the logarithmic growth stage, which shows a sharp increase in cell mass. After 48 hours, the bacteria were observed to enter the stationary phase. However, medium containing S4 extract 0.1% (w / v) remained in the induction phase for up to 60 hours and showed a gradual increase in cell weight but no rapid increase in bacteria. This suggests that unclear cleanliness inhibits the growth of P. acnes even at low concentrations. No growth of bacteria was observed in the medium with more than 0.5% uncleaned eye. This means that 0.5% of unclean solution can completely inhibit the growth of P.acnes . In addition, it is considered that the extract of Unbleached Cleansing Oil can be used as a growth inhibitory substance against P. acnes because it has a considerably high growth inhibitory activity against the bacterium.

Stapylococcus aureus is often reported to form colonies in acne dermatitis and is related to the severity of acne dermatitis. Therefore, the focus is generally on reducing or killing the cells using antibiotics as well as chemical fungicides. However, most of them require new forms of antimicrobial agents due to their resistance to antibiotics and the cytotoxicity of chemical fungicides. Therefore, we tried to find out the antimicrobial effect of the fungus using the extract of undamaged cleansing solution in this experiment.

Figure 3. Growth inhibition effect of Stapylococcus aureus at various concentrations in unbleached clear eyes.

Figure 3 shows the effects of uncleaned solution on the growth of Stapylococcus aureus by measuring the OD value over time. Stapylococcus aureus showed faster growth rate than the other microorganisms used in the experiment, and showed a logarithmic growth period up to 12 hours after 1 hour induction at 1 × 10 ⁶ cfu / ml inoculation. No specific inhibitory activity was observed in the medium containing 0.1% to 1.0% of unclear cleanse, but as the concentration of uncleaned cleared was increased, the microorganisms delayed the time to enter the logarithmic growth phase from the induction phase and lowered the maximum cell weight in the culture. This shows that the higher the concentration of uncleaned eye drops, the more inhibitory effect the Stapylococcus aureus has on growth. In addition, unclear cleansing inhibits the growth of Stapylococcus aureus , but indicates that high concentration treatment is required to have an effect.

Experimental Example 3. Cytotoxicity Test of Unbleached Clean Solution

For cytotoxicity test of unbleached clear eye of Preparation Example 1, the cell line was a L929 cell line which is a kind of mouse fibroblasts. Cell culture was performed in Dulbecco's Modified Eagle's Medium (DEME) medium containing 10% fetal bovine serum (FBS) and 1% of 100 units / ml penicillin / streptomycin, and cultured in an incubator at 37 ° C. and 5% CO ₂ . The culture is changed every 3 days. The cultured cells were suspended with 1 × trypsin-EDTA and stained with 0.4% trypan blue to count the number of cells with a hemocytometer. Cytotoxicity test is used in the experiment after culturing the cultured cells by dispensing 120μl of cell suspension to 96 × 96 well plate to 5 × 10 ³ cells / well.

Cell lines cultured according to Mosmann's method were dispensed into 96 well plates to be 5 × 10 ³ cells / ml, respectively, and the cells were cultured for 24 hours and then S1 at 1, 10, 50, 100, 200, 500, 1000 ppm concentrations After incubation for 24 hours in the included culture medium 200μl / well containing MTT 200μl / ㎖ and then incubated for 3 hours again. After incubation, the culture medium was discarded, and 100 μl / well of DMSO was added thereto and left at room temperature for 5 minutes. After dissolving Formazan, the absorbance was measured at 570 nm with a microplate reader.

By using the MTT quantitative method, L929 cells were cultured in culture medium containing uncleaned solution at concentrations of 1, 10, 50, 100, 200, 500, and 1000 ppm for 24 hours, and then compared with the control group (Fig. 4). In the 100 ppm concentration of undamaged cleansing, the cell growth rate was increased to 3% compared to the control group, and it was confirmed that the increase was 7% at 200 ppm. At 500 ppm and 1000 ppm, cell viability decreased below 6 and 13% compared to the control group. It was found that unclear cleansing induced cytotoxicity above 500 ppm concentration in L929 cells.

Figure 4. Cytotoxicity test of various concentrations of unbleached clear eye in L929 cell line.

Preparation Example 2 Preparation of Cosmetic Composition Containing Unbleached Clean Solution

In order to prepare a cosmetic composition as a composition comprising the unbleached clean solution prepared by Preparation Example 1, a raw material as described in Table 2 below was prepared. First, raw materials No. 1 to 7 of Table 2 were mixed and dissolved at 70 ° C. to prepare a first part. In addition, raw materials No. 8 to 14 of Table 2 were mixed and dissolved at 70 ° C. to prepare a second part.

It was. The prepared second part was added to the first part, stirred first using a homomixer (Tokushu Kika, Japan) and then emulsified by adding raw material No. 15 in Table 2 below. Subsequently, bubbles were removed from the increased composition, and then cooled to room temperature to prepare a cosmetic composition of the cosmetic preparation.

Raw material number ingredient Addition amount (g) One Purified water 100 2 glycerin 8 3 Butylene glycol 4 4 Hyaluronic acid extract 5 5 Beta Glucan 7 6 Carbomer 0.1 7 Unclear cleanliness prepared by Preparation Example 1 3 8 Caprylic Gaprik Triglycerides 8 9 Squalene 5 10 Cetearyl Glucoside 1.5 11 Sorbitan stearate 0.4 12 Cerearyl alcohol One 13 antiseptic Quantity 14 Herb flavor Quantity 15 Triethanolamine 0.1

Comparative Production Example 1. Preparation of cosmetic composition containing no unclean clear solution

Except not mixing the raw material No. 7 of Table 2 to prepare a cosmetic composition according to Comparative Preparation Example 1 in the same manner as in Preparation Example 3.

Experimental Example 4. Stability test of the cosmetic composition

In order to confirm the safety of the cosmetic composition according to Preparation Example 2, Comparative Preparation Example 1 was carried out a human skin cumulative stimulation test. Specifically, a total of nine 24-hour cumulative patches were performed every other day on the upper forearm for each of the cosmetic compositions according to Preparation Example 2 and Comparative Preparation Example 1 for 20 healthy adult men and women. As the patch method, a Fin chamber method was used, and 15 µl of the cosmetic composition was dropped per chamber, and the patch was performed. The reactivity of the skin was divided into ±, +, and ++ according to the degree, and 1, 2, and 4 (highest scores) were given to each. In addition, the average reactivity was calculated according to Equation 6 below, and is shown in Table 3 together with the results of the reactivity. In Table 3 below it is determined to be a safe composition when the average reactivity is less than 3.

Equation 1

Average responsiveness = (number of respondents x responsiveness / total number of subjects x highest score x 100) / total number of tests

exam
matter Number of subjects with response Average responsiveness 1 week 2 weeks 3 weeks Primary Secondary 3rd 4th 5th 6th 7th 8th 9th ± + ++ ± + ++ ± + ++ ± + ++ ± + ++ ± + ++ ± + ++ ± + ++ ± + ++ Production Example 2 - - - - - - - - - - - - - - - - - - - - - - - - - - - 0.00 Comparative Preparation Example 1 - - - - - - - - - - - - - - - - - - - - - - - - - - - 0.00

"-" In Table 3 indicates that there is no corresponding person. As can be seen from Table 3, the cosmetic composition prepared by the method according to Preparation Example 2 showed no cumulative stimulation at all like the cosmetic composition according to Comparative Preparation Example 1. Therefore, it can be seen that the cosmetic composition according to Preparation Example 2 has safety.

Claims (7)

Antibacterial, regenerating, whitening improvement and cosmetic herbal composition of acne skin containing complex herbal extracts including red ginseng, baekbokyeong, saengjihwang, white wheat, makmundong, astronomical dong, phalanx, sulfur, turmeric, yellow lotus, gold, gardenia and wormwood as active ingredients .

The method of claim 1,
Red ginseng, Baekbokryeong, Saengjihwang, Baekmil, Mcmundong, Astronomical Dong, Jigolpi, Sulfur, Turmeric, Huangyan, Golden, Gardenia and Mugwort, individual weight composition ratio of 1-10: 1-10: 1-10: 1-10: 0.1-10: 0.1 ~ 1: 0.1 ~ 1: 1 ~ 5: 0.1 ~ 1: 0.1 ~ 1: 0.1 ~ 1: 0.1 ~ 1: 0.1 ~ 1 Naturally derived herbal composition.
The herbal composition for antibacterial, regenerating, whitening improvement and prevention of acne skin containing uncleaned clear solution of the present invention comprises 0.1 to 50% by weight of the extract based on the total weight of the composition.

A cosmetic composition comprising an unclear cleansing composition, wherein the uncleaning clearing composition has a content of 0.0001 to 30% by weight based on the total weight of the composition.

The method of claim 3, wherein
The composition is a cosmetic composition comprising one formulation selected from the group consisting of a lotion, essence, lotion, cream, pack, gel, ointment, patch and spray.

The method of claim 3, wherein
A composition for cosmetics further comprising at least one selected from the group consisting of alcohols, oils, surfactants, fatty acids, silicone oils, wetting agents, moisturizers, viscosity modifiers, emulsions, stabilizers, sunscreens, colorants and perfumes.

A soap composition comprising an unclean clean solution, wherein the unclean clean solution has a content of 0.0001 to 30% by weight based on the total weight of the composition.

The method of claim 6,
A soap composition further comprising at least one of a soap base, a skin moisturizer, an emulsifier, and a water softener.

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