CN117837424A - A method for controlling crop Laodelphax striatellus - Google Patents

Abstract

The present invention provides a method for controlling gray leafhoppers on crops, and relates to the technical field of pest control. The present invention effectively controls the occurrence and development of gray leafhoppers on crops through an insecticide resistance management strategy combined with a specific insecticide composition, protects the growth and yield of crops, avoids the use of a single insecticide leading to enhanced resistance of gray leafhoppers, can reduce the amount and cost of insecticides, and reduce pollution and harm to the environment and human body.

Description

A method for controlling crop sporehoppers

Technical Field

The invention relates to the technical field of pest control, and in particular to a method for controlling the brown planthopper of crops.

Background technique

The gray leafhopper is a major pest that harms crops. It mainly sucks the leaves and stems of crops, causing nutrient loss in rice and affecting rice growth and yield. At the same time, the secretions of the gray leafhopper can also contaminate the leaves of crops, leading to the occurrence of diseases such as anthrax. The occurrence and development of the gray leafhopper is affected by environmental factors such as temperature, humidity, and light.

At present, the main method of controlling gray leafhoppers is to use chemical insecticides for spray control. Commonly used chemical insecticides include neurotoxic insecticides, mesoionic insecticides and cholinergic neurotoxic insecticides. Neurotoxic insecticides mainly interfere with the nerve conduction of pests, causing them to paralyze or die. Mesoionic insecticides mainly destroy the cell membrane of pests, causing them to lose their physiological functions. Cholinergic neurotoxic insecticides mainly inhibit the acetylcholinesterase of pests, causing their nervous system to be overexcited and die. These chemical insecticides each have their advantages and disadvantages, but if a single insecticide is used, it is easy to cause gray leafhoppers to develop resistance, reduce the insecticidal effect, and also increase the dosage and cost of insecticides, causing greater pollution and harm to the environment and human body. Therefore, there is a need for a method for controlling crop leafhoppers that can reasonably use insecticides with different mechanisms of action, avoid the resistance of gray leafhoppers, improve the insecticidal effect, reduce the dosage and cost of insecticides, and reduce the pollution and harm to the environment and human body to overcome the problems existing in the existing prevention and control of gray leafhoppers in crops.

Summary of the invention

To solve the above problems, the present invention provides a method for controlling gray leafhoppers on crops. The method can effectively control the occurrence and development of gray leafhoppers on crops and protect the growth and yield of crops through an insecticide resistance management strategy combined with a specific insecticide composition; it can reasonably use insecticides with different mechanisms of action to avoid the use of a single insecticide that leads to enhanced resistance of gray leafhoppers; it can reduce the dosage and cost of insecticides and reduce pollution and harm to the environment and human body.

The method for controlling the crop Laodelphax striatellus of the present invention comprises the following steps:

S1. Use pesticides for spraying in the early stages of crop growth to control the initial occurrence of Laodelphax striatellus;

S2. Use pesticides for spraying in the middle of crop growth to control the high incidence period of Laodelphax striatellus;

S3. In the later stage of crop growth, use pesticides for spraying to eliminate the remaining Laodelphax striatellus;

Each spray contains 0.1% to 3% of verapamil hydrochloride, which improves the killing effect of gray leafhoppers, reduces the amount of insecticides used and prevents gray leafhoppers from developing resistance.

The crops are one or more of rice, corn, oats, wheat and barley.

Furthermore, the insecticide is one or more of acetamiprid, clothianidin, nitenpyram, imidacloprid, ethiprole, sulfoxaflor, spinosad, spinetoram, avermectin, lepidomycin, buprofezin, pymetrozine, and fipronil.

Furthermore, the insecticide is one or more of clothianidin, nitenpyram, sulfoxaflor, buprofezin, and pymetrozine.

Furthermore, the insecticide is one or more of wettable powder, suspension, emulsion and aqueous solution.

Compared with the prior art, the beneficial technical effects of the present invention are:

The present invention can effectively control the occurrence and development of crop ligninators by combining a specific insecticide composition with an insecticide resistance management strategy, and protect the growth and yield of crops. The insecticide composition of the present invention includes insecticides with different action mechanisms, which can produce synergistic or antagonistic effects on different physiological processes and targets of ligninators, enhance insecticidal effects, and reduce resistance risks.

The present invention adds a small amount of verapamil hydrochloride to the pesticide, which can increase the toxicity of the pesticide, significantly enhance the effect of the pesticide, reduce the dosage of the pesticide, and avoid the generation of resistance. At the same time, it can also reduce the impact of the pesticide on non-target organisms and reduce environmental pollution.

The method of the invention is applicable to a variety of crops and can be widely used in agricultural production to improve the quality and yield of crops, increase farmers' income and promote agricultural development.

Detailed ways

The invention provides a method for controlling gray leafhoppers in crops, which is achieved by combining an insecticide resistance management strategy with a specific insecticide composition.

The method for controlling the crop Laodelphax striatellus of the present invention comprises the following steps:

S1. Use pesticides for spraying in the early stages of crop growth to control the initial occurrence of Laodelphax striatellus;

S2. Use pesticides for spraying in the middle of crop growth to control the high incidence period of Laodelphax striatellus;

S3. In the later stage of crop growth, use pesticides for spraying to eliminate the remaining Laodelphax striatellus;

Each spray contains 0.1% to 3% of verapamil hydrochloride, which improves the killing effect of gray leafhoppers, reduces the amount of insecticides used and prevents gray leafhoppers from developing resistance.

The crops are one or more of rice, corn, oats, wheat and barley.

In one embodiment, the insecticide is one or more of acetamiprid, clothianidin, nitenpyram, imidacloprid, ethiprole, sulfoxaflor, spinosad, spinetoram, avermectin, lepidomycin, buprofezin, pymetrozine, and fipronil.

In one embodiment, the insecticide is one or more of cypermethrin, clothianidin, nitenpyram, sulfoxaflor, buprofezin, and pymetrozine.

In one embodiment, the pesticide is one or more of a wettable powder, a suspension, an emulsion, and an aqueous solution.

In the insecticide of the present invention, acetamiprid, clothianidin, nitenpyram, imidacloprid, ethiprole, sulfoxaflor are all neonicotinoid insecticides, which mainly act on the nicotinic acetylcholine receptors of insects, overactivate them, cause excitement of the nervous system, and cause insect paralysis, refusal to feed, and death. The structures of these insecticides all contain pyridine or thiazole rings, but the specific action sites and lipophilicity are different, which affects their activity and selectivity. Spinosad, ethyl spinetoram, avermectin, and lepidomectin are all biological insecticides, produced by actinomycetes, and mainly act on the glutamate-gated chloride channels of insects, increase the chloride ion permeability of nerve cells, and cause insect paralysis or death. The structures of these insecticides all contain macrolide rings, but the specific substituents and sugar groups are different, which affects their stability and water solubility. Buprofezin is a pyridine (pyridine imine) or triazone insecticide that mainly acts on the insect's unique proprioceptor, the chord organ, causing behavioral disorders in insects, causing them to stop feeding and eventually starve to death. Buprofezin contains a thiazine ring and a ketone group in its structure, which has high lipophilicity and stability. Pymetrozine is a neonicotinoid insecticide that mainly acts on the nicotinic acetylcholine receptors of insects, causing them to overactivate, causing excitement of the nervous system, leading to paralysis, refusal to feed, and death of insects. Pymetrozine contains a thiazole ring and a thioether bond in its structure, which has high activity and selectivity. Fipronil is a new type of phenylurea insecticide that mainly acts on the Reye's muscle calcium ion channel of insects, interfering with muscle contraction, causing paralysis or death of insects. Fipronil contains a fluorine atom and an amide bond in its structure, which has high lipophilicity and stability.

It can be seen that different types of insecticides have their own advantages and disadvantages. Therefore, it is difficult to achieve good control effects by using a single type of insecticide to control gray leafhoppers in crops, and it is easy for gray leafhoppers to develop resistance, making subsequent control work difficult. Therefore, in order to effectively control gray leafhoppers, a variety of insecticides should be reasonably selected and combined with specific insecticide resistance management strategies to improve insecticidal effects, reduce resistance risks, reduce environmental pollution, and reduce the impact on non-target organisms.

In addition, a small amount of verapamil hydrochloride is added to the insecticide in the present invention. Verapamil hydrochloride can inhibit the ABC transporter protein of the gray leafhopper to significantly enhance the effect of the insecticide, reduce the amount of the insecticide, and avoid the generation of resistance.

In addition, it was found during the research process that the insecticide composition and insecticide adjuvant of the present invention are only applicable to Hemiptera pests, preferably Laodelphax striatellus, and have limited control effects on other types of pests, such as flies, moths, mosquitoes and flies. Therefore, the method of the present invention is mainly used for the control of Laodelphax striatellus in crops.

The technical solution provided by the present invention is further described below in conjunction with embodiments.

Example 1

In the early stage of rice growth, use 10% cypermethrin wettable powder for preventive spraying, with a dosage of 20g per mu and 20 kg of water for spraying to control the initial occurrence of gray leafhoppers. In the middle stage of rice growth, use a mixture of 10% thiamethoxam wettable powder and 10% pymetrozine wettable powder, with a dosage of 20g and 12g per mu respectively, and 15-20 kg of water for control spraying to control the high incidence period of gray leafhoppers. In the late stage of rice growth, use 25% clothianidin wettable powder for field cleaning spraying, with a dosage of 15g per mu and 40 kg of water to eliminate the remaining gray leafhoppers.

Each spray contains 0.3% verapamil hydrochloride, which improves the killing effect of gray leafhoppers, reduces the amount of insecticides used and prevents gray leafhoppers from developing resistance.

Example 2

In the early stage of rice growth, use 10% sulfoxaflor wettable powder for preventive spraying, with a dosage of 16g per mu and 20 kg of water to control the initial occurrence of gray leafhoppers. In the middle stage of rice growth, use a mixture of 10% thiamethoxam wettable powder and 10% pymetrozine wettable powder for control spraying, with a dosage of 20g and 15g per mu respectively, and 20 kg of water to control the high incidence period of gray leafhoppers. In the late stage of rice growth, use nitenpyram wettable powder for field cleaning spraying, with a dosage of 10g per mu and 20 kg of water to eliminate the remaining gray leafhoppers.

Each spray contains 0.5% verapamil hydrochloride, which improves the killing effect of gray leafhoppers, reduces the amount of insecticides used and prevents gray leafhoppers from developing resistance.

Example 3

In the early stage of rice growth, use 10% nitenpyram wettable powder for preventive spraying, with a dosage of 10g per mu and 20 kg of water to control the initial occurrence of gray leafhoppers. In the middle stage of rice growth, mix 10% thiamethoxam wettable powder and 25% clothianidin wettable powder for control spraying, with a dosage of 25g and 15g per mu respectively and 20 kg of water to control the high incidence period of gray leafhoppers. In the late stage of rice growth, use 10% sulfoxaflor wettable powder for field cleaning spraying, with a dosage of 16g per mu and 15 kg of water to eliminate the remaining gray leafhoppers.

Each spray contains 1.2% verapamil hydrochloride, which improves the killing effect of gray leafhoppers, reduces the amount of insecticides used and prevents gray leafhoppers from developing resistance.

Test Example 1

The rice seedlings were immersed in a certain concentration of insecticide composition solution, and then the rice seedlings were naturally dried until there was no liquid on the surface, and then covered with a PC tube, and the 3rd-instar nymphs of the gray planthopper were transferred to the rice seedlings. Each concentration was repeated 3 times, and the temperature was maintained at 25°C and the humidity was 75%. The number of dead insects was recorded after 72 hours. After testing, the LC ₅₀ of different insecticide compositions is as follows:

LC ₅₀ 95% Confidence Interval Resistance Ratio (RR) Efficiency ratio Cyclocloprid 8.37 5.101-12.094 247.3 — Cyclocloprid + Verapamil 2.82 1.723-4.574 226.5 2.97 Clothianidin 5.38 2.976-8.111 40.6 — Clothianidin + Verapamil 2.38 1.628-3.265 31.1 2.26 Nitenpyram 1.69 0.772-2.174 2.2 — Nitenpyram + Verapamil 0.84 0.453-1.729 1.3 2.01 Sulfoxaflor 1.98 1.022-3.532 5.0 — Sulfoxaflor + Verapamil 1.11 0.873-1.656 3.8 1.79 Buprofezin 155.26 80.114-215.621 103.5 — Buprofezin + Verapamil 63.37 30.199-103.251 88.4 2.45 Pymetrozine 13.01 7.213-18.582 3.2 — Pymetrozine + Verapamil 6.41 3.608-9.141 2.6 2.03

Note: Resistance multiple: RR≤5.0, sensitive level; 5.0<RR≤10.0, low level resistance; 10.0<RR≤100.0, medium level resistance; RR>100, high level resistance.

Resistance ratio (RR) = _LC50 value/ _LC50 value of sensitive population.

Test Example 2

The test method was appropriately adjusted according to the different animal species, and a similar method was used to determine the synergistic ratio of the composition of Test Example 1. The results are as follows:

(1) Spodoptera litura larvae:

(2) Leafminer

Efficiency ratio Cyclocloprid — Cyclocloprid + Verapamil 0.453 Clothianidin — Clothianidin + Verapamil 0.537 Nitenpyram — Nitenpyram + Verapamil 0.256 Sulfoxaflor — Sulfoxaflor + Verapamil 0.603 Buprofezin — Buprofezin + Verapamil 0.072 Pymetrozine — Pymetrozine + Verapamil 0.038

The control effects of thiamethoxam and pymetrozine on Spodoptera litura and leafminer are not obvious, and they are generally not used for the control of Spodoptera litura and leafminer, so the synergistic effect is also very limited.

The principles and implementation methods of the present invention are described in this article using specific examples. The description of the above embodiments is only used to help understand the method and core ideas of the present invention. At the same time, for those skilled in the art, according to the ideas of the present invention, there will be changes in the specific implementation methods and application scope. In summary, the content of this specification should not be understood as limiting the present invention.

Claims (5)

1. A method for controlling crop Laodelphax striatellus, comprising the following steps: S1. Use pesticides for spraying in the early stages of crop growth to control the initial occurrence of Laodelphax striatellus; S2. Use pesticides for spraying in the middle of crop growth to control the high incidence period of Laodelphax striatellus; S3. In the later stage of crop growth, use pesticides for spraying to eliminate the remaining Laodelphax striatellus; Each spray contains 0.1% to 3% verapamil hydrochloride. 2. A method for controlling brown planthoppers in crops according to claim 1, characterized in that the crops are one or more of rice, corn, oats, wheat, and barley. 3. A method for controlling the brown planthopper of crops according to claim 1, characterized in that the insecticide is one or more of acetamiprid, clothianidin, nitenpyram, imidacloprid, ethiprole, sulfoxaflor, spinosad, spinetoram, avermectin, lepidomycin, buprofezin, pymetrozine, and fipronil. 4. A method for controlling the brown planthopper of crops according to claim 3, characterized in that the insecticide is one or more of clothianidin, nitenpyram, sulfoxaflor, buprofezin, and pymetrozine. 5. A method for controlling the brown planthopper of crops according to claim 1, characterized in that the insecticide is one or more of a wettable powder, a suspension, an emulsion, and an aqueous solution.