CN117694341A - Application of ricinine, rodenticide and preparation method and application thereof - Google Patents
Application of ricinine, rodenticide and preparation method and application thereof Download PDFInfo
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N25/00—Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests
- A01N25/002—Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests containing a foodstuff as carrier or diluent, i.e. baits
- A01N25/004—Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests containing a foodstuff as carrier or diluent, i.e. baits rodenticidal
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N25/00—Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests
- A01N25/12—Powders or granules
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/34—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one nitrogen atom as the only ring hetero atom
- A01N43/40—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one nitrogen atom as the only ring hetero atom six-membered rings
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01P—BIOCIDAL, PEST REPELLANT, PEST ATTRACTANT OR PLANT GROWTH REGULATORY ACTIVITY OF CHEMICAL COMPOUNDS OR PREPARATIONS
- A01P11/00—Rodenticides
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
- Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change
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Abstract
The invention relates to the technical field of biology, in particular to application of ricinine, a rodenticide, a preparation method and application thereof. The invention provides application of ricinine in preparation of rodenticide, wherein the effective concentration of ricinine is 0.45-1.80%; the invention also provides a rodenticide which comprises ricinine, base bait, a stabilizer and a bittering agent. The ingestion coefficient of the rodenticide prepared by the invention is more than 0.52, the rodenticide rate can reach 100% when the laboratory tests the rodenticide on the 9 th day, and the field test results show that the rodenticide rate reaches 95.17% when the rodenticide is 21 days; the rodenticide provided by the invention is safe to use, store and transport, and has qualified stability.
Description
Technical Field
The invention relates to the technical field of biology, in particular to application of ricinine, a rodenticide, a preparation method and application thereof.
Background
The current rodenticide is mostly anticoagulation rodenticide, or 4-hydroxycoumarin, or indandiones such as rodenticide ether, rodenticide, diphacinone, bromadiolone, triad, etc., and the principle is that vitamin K can be competitively inhibited in the body of the mice to block the synthesis of prothrombin, thereby causing fatal internal hemorrhage to die.
In addition, there are a small number of fumigating rodenticides such as chloropicrin, aluminum phosphide, acute rodenticide fluoroacetamide and the like which are used in specific places such as small-sized closed environments and the like, and the toxicity is forbidden due to the too high.
With the wide application of chemical rodenticides, feeding refusal occurs, so that the anticoagulation rodenticide has reduced control effect. With the improvement of environmental safety requirements and the development of technology, biological rodenticides are important points of future research and development, for example, C-type botulinum toxin has good effect of preventing and killing mice such as plateau mice, rabbits, zokors and the like in field tests, and has been registered. But few alternative biological rodenticides.
Disclosure of Invention
The invention aims to provide application of ricinine, and the rodenticide prepared from ricinine has the characteristics of obvious rodenticide effect, environment friendliness, easiness in degradation, no residue, high ecological compatibility and simple preparation process.
In order to achieve the above object, the present invention provides the following technical solutions:
the invention provides application of ricinine in preparation of rodenticide.
Preferably, the effective concentration of ricinine is 0.45-1.80%.
The invention also provides a rodenticide, which comprises the following components in parts by weight: 0.45-1.80 parts of ricinine, 97-99 parts of base bait, 0.1-0.3 part of stabilizer and 0.002-0.02 part of bittering agent.
Preferably, the base bait is one or more of corn flour, milk powder, peanut cake and vegetable oil; the stabilizer is dibutyl hydroxyl toluene; the bittering agent is benidialium.
The invention also provides a preparation method of the rodenticide, which comprises the following steps:
(1) Mixing ricinine, base bait, stabilizer and bittering agent to obtain a mixture;
(2) Granulating the mixture, and drying to obtain the rodenticide.
Preferably, the drying method is dryer heating; the temperature of the drying is 55-60 ℃.
Preferably, the parameters in the granulation process are set as follows: the feeding speed is 10-20 kg/min, the rotating speed is 8-10 r/min, the discharging speed is 10-20 kg/min, and the pressure is 2-3 kg/cm 2 The temperature is 20-30 ℃.
The invention also provides application of the rodenticide in preventing and controlling rodenticide.
The invention also provides application of the rodenticide prepared by the preparation method in preventing and controlling rodenticide.
The invention has the beneficial effects that:
the invention adopts ricinine to prepare the plant source rodenticide to control the domestic mice, which belongs to the first time in China at present, and has remarkable social benefit, especially environmental benefit.
According to the invention, ricinine is used for preparing a plant source rodenticide, through experimental research, the effective concentration of the ricinine is in the range of 0.45-1.8%, wherein the 0.9% mortality rate and palatability are optimal, in laboratory experiments, rats reach a death peak in 5-6 days, the 9 th death rate reaches 100%, the ingestion coefficient is 0.59, and the requirements of the A-level pharmacodynamic standard of not less than 0.3 are met; the field test results show that the rate of killing mice in 21 days reaches 95.17 percent.
The extracted ricinine (effective concentration 10%) has acute oral LD on rats 50 68.26-76.96 mg/kg, is moderate in toxicity, has no acute irritation to rabbit skin and light irritation to rabbit eyes, and is safe to use, store and transport.
The rodenticide prepared by the method has the advantages of heat storage at 54+/-2 ℃ and 14d decomposition rate of 4.30%, less than 10% of requirements, and qualified stability.
Detailed Description
The invention provides application of ricinine in preparation of rodenticide.
In the invention, the effective concentration of ricinine is 0.45-1.80%, preferably 1.125%.
The invention also provides a rodenticide, which comprises the following components in parts by weight:
0.45 to 1.80 parts, preferably 1.125 parts, of ricinine,
97 to 99 parts of base bait, preferably 98 parts,
0.1 to 0.3 part, preferably 0.2 part, of stabilizer,
0.002 to 0.02 parts, preferably 0.011 parts of bittering agent.
In the invention, the base bait is one or more of corn flour, milk powder, peanut cake and vegetable oil, and the stabilizer is dibutyl hydroxy toluene; the bittering agent is benidialium.
In the invention, when the base bait is corn flour, milk powder, peanut cake and vegetable oil, the mass ratio of the corn flour to the milk powder to the peanut cake to the vegetable oil is 40-44:0.5-1.5:2-4:3-5, preferably 42:1:3:4.
In the invention, the extraction process of ricinine comprises the following steps: adding 5L of deionized water into 500g of castor meal, decocting for 3h, filtering, and placing the filtrate at 25 ℃ to obtain ricinine extracting solution; the ricinine extracting solution passes through a D101 macroporous adsorption resin column, the volume of the macroporous adsorption resin column is 500mL, the diameter is 5cm, the diameter-to-diameter ratio is 1:10, after the adsorption is finished, 4BV is eluted by water at the flow rate of 3.5BV/h for removing impurities, 4BV is eluted by 32% ethanol, 32% ethanol eluent is collected, and the solvent is recovered to 50mL under reduced pressure at 67 ℃ and minus 0.12MPa, thus obtaining the ricinine.
The invention also provides a preparation method of the rodenticide, which comprises the following steps:
(1) Mixing ricinine, base bait, stabilizer and bittering agent to obtain a mixture;
(2) Granulating the mixture, and drying to obtain the rodenticide.
In the invention, the drying method is that the dryer heats; the temperature of the drying is 55-60 ℃, preferably 57.5 ℃.
In the present invention, the parameters in the granulation process are set as follows: the feeding speed is 10-20 kg/min, preferably 15kg/min, the rotating speed is 8-10 r/min, preferably 9r/min, the discharging speed is 10-20 kg/min, preferably 15kg/min, and the pressure is 2-3 kg/cm 2 Preferably 2.5kg/cm 2 The temperature is 20 to 30 ℃, preferably 25 ℃.
The invention also provides application of the rodenticide in preventing and controlling rodenticide.
The invention also provides application of the rodenticide prepared by the preparation method in preventing and controlling rodenticide.
The technical solutions provided by the present invention are described in detail below with reference to examples, but they should not be construed as limiting the scope of the present invention.
Example 1 rodenticide
Extracting ricinine: adding 5L of deionized water into 500g of castor meal, decocting for 3h, filtering, and placing the filtrate at 25 ℃ to obtain ricinine extracting solution; the ricinine extracting solution passes through a D101 macroporous adsorption resin column, the volume of the macroporous adsorption resin column is 500mL, the diameter is 5cm, the diameter-to-diameter ratio is 1:10, after the adsorption is finished, 4BV is eluted by water at the flow rate of 3.5BV/h for removing impurities, 4BV is eluted by 32% ethanol, 32% ethanol eluent is collected, and the solvent is recovered to 50mL under reduced pressure at 67 ℃ and minus 0.12MPa, thus obtaining the ricinine.
The preparation method of the rodenticide comprises the following steps:
(1) 0.45kg of ricinine, 86kg of corn meal, 8kg of vegetable oil, 1kg of milk powder, 4kg of peanut cake and 0.30kg of dibutyl hydroxy toluene; 0.02kg of benidiammonium to obtain a mixture;
(2) The mixture is fed at a speed of 13kg/min, a speed of 8r/min, a discharge speed of 13kg/min and a pressure of 2kg/cm 2 Granulating at 20 ℃, and heating and drying by a dryer at 60 ℃ to obtain the rodenticide.
Detecting by high pressure liquid chromatography, wherein the effective concentration of ricinine obtained by extraction is 10%; the effective concentration of ricinine in rodenticide is 0.45%.
Example 2 rodenticide
The step of extracting ricinine is described in example 1;
the preparation method of the rodenticide comprises the following steps:
(1) 0.9kg of ricinine, 82kg of corn meal, 8kg of vegetable oil, 2kg of milk powder, 6kg of peanut cake and 0.25kg of dibutyl hydroxy toluene; 0.018kg of benidiammonium;
(2) The mixture is fed at a feeding speed of 15kg/min, a rotating speed of 9r/min, a discharging speed of 15kg/min and a pressure of 3kg/cm 2 Granulating at 25 ℃, and heating and drying by a 55 ℃ dryer to obtain the rodenticide.
The effective concentration of ricinine in rodenticide is 0.90% by high pressure liquid chromatography detection.
Example 3 rodenticide
The step of extracting ricinine is described in example 1;
the preparation method of the rodenticide comprises the following steps:
(1) 1.8kg of ricinine, 82kg of corn meal, 6kg of vegetable oil, 3kg of milk powder, 8kg of peanut cake and 0.20kg of dibutyl hydroxy toluene; 0.01kg of benidiammonium;
(2) The mixture was fed at a feed rate of 17kg/min, a rotational speed of 10r/min, a discharge rate of 17kg/min and a pressure of 2.5kg/cm 2 Granulating at 30 ℃, and heating and drying by a dryer at 57 ℃ to obtain the rodenticide.
The effective concentration of ricinine in rodenticide is 1.80% by high pressure liquid chromatography detection.
Experimental example 1 deratization test
1. Laboratory test
1.1 test mice: 40 SD rats, male and female halves (purchased from the university of medical science laboratory animal center, lanzhou, weight 100-120 g).
1.2 test method: referring to "method for testing efficacy of rodenticide for pesticide registration to control domestic mice and evaluation" NY/T1152-2006, 4 cages were set up, respectively as example 1 group, example 2 group, example 3 group, and control group, each cage was randomly placed with 10 normal SD rats, each half of male and female. 50g of the rodenticide described in examples 1-3 and 50g of the mouse feed were placed in the cages symmetrically, respectively, and the positions of bait consumption, replenishment and exchange of bait boxes were recorded at regular daily intervals. And continuously observing for 7d, removing rodenticide, recovering normal feeding for 14d, observing and recording daily death condition, and calculating the feeding coefficient and death rate. A control group was set, which was fed only, and the rest was the same.
The calculation formula is as follows:
murine density = positive number of powder pieces/number of effective powder pieces,
mortality = (pre-test murine density-post-test murine density)/pre-test murine density,
feeding coefficient = rodenticide consumption/feed consumption, grading standard: class a: ingestion coefficient > 0.3, grade b: the ingestion coefficient is more than or equal to 0.1 and less than or equal to 0.3;
corrected mortality = (example mortality-control mortality)/(1-control mortality), grading criterion: class a: corrected mortality > 90%, grade B: the correction mortality rate is more than or equal to 80% and less than or equal to 90%.
1.3 experimental results: the results of the indoor drug efficacy measurement are shown in table 1.
TABLE 1 results of indoor efficacy measurements of ricinine rodenticides at different concentrations
As can be seen from Table 1, in examples 1 to 3, rats reached a peak of death on days 5 to 8 and had a mortality rate of 90% or more on days 9 to 12, and the rat killing effect was remarkable; the ingestion coefficients are all above 0.52, and are higher than the requirements of the drug effect standard ingestion coefficient class A of 0.30, which indicates that the rodenticide has good palatability;
the effect of the rodenticide is most remarkable when the effective concentration of ricinine is 0.9%, the feeding coefficient is 0.59, and the death rate of rats reaches 100% at 9 d.
2. Outdoor test
2.1 test address: the Sichuan Luzhou pig farm is composed of 110 pig houses, 9 pig houses with higher visual mouse density are selected, 3 pig houses are selected randomly, and the pig houses respectively represent a test area (the bait put in is rodenticide described in example 1), a control medicament area (the bait put in is 0.005% bromadiuron wax block rodenticide, purchased from Guangxi Long Huaxin sanitary insecticide Co., ltd., lot number 20210912) and a blank control area (equal amount of pig feed is put in a bait put-in mode).
2.2 test method: the powder method is calculated by 300 blocks, and the positive rate is more than or equal to 20 percent. The pre-use powder method of the experiment investigated the density of mice (positive powder/effective powder) for dominant species of mice. Every 15m of the room in the test area 2 3 stacks of the corresponding baits (as described in 2.1) were placed, and 1 stack of rodenticide described in example 1 was placed at 8m intervals outdoors, 15g each stack, and placed in a bait box. Continuous 5 days of feeding and checking bait consumption and supplementation. The administration mode of the control medicament area and the blank control area is the same as that of the control medicament area. The densities of the test and control areas were investigated 21 days after feeding, using x 2 The test pair was statistically analyzed, with P > 0.05, without differences.
2.3 detection results: the results of the on-site drug effect detection are shown in Table 2.
TABLE 2 results of in situ comparative efficacy test of ricinine rodenticide
As can be seen from table 2, the rodenticide described in example 1 has a good rodenticide effect with the control agent, and the corrected rodenticide rate is 95.17% which is consistent (no statistical difference) with the rodenticide of the control agent.
Experimental example 2 stability test
The method is carried out according to the method related to the method for measuring the heat storage stability of pesticides in GB/T19136-2021. 20g of the rodenticides described in examples 1 to 3 (corresponding to samples 1 to 3, respectively) were stored in 25mL test tubes, placed in an incubator at a set temperature of 54 ℃ + -2 ℃ and after 14 days of heat storage, the content of the active ingredient after heat storage of the samples was detected, and compared with the content of the active ingredient before heat storage, the decomposition rate of heat storage was calculated, and the decomposition rate of heat storage= (content before heat storage-content after heat storage)/content before heat storage, the results are shown in Table 3.
TABLE 3 results of thermal storage stability test of ricinine rodenticide samples
The result shows that the product has the average decomposition rate of 4.30 percent at the temperature of 54+/-2 ℃ and 14 days, less than 10 percent, and qualified stability.
Experimental example 3 toxicology test
The detection basis is as follows: GB15670-1995 method for the registered toxicology test of pesticides.
1. Acute oral toxicity test
1.1 test animals: SD species of rats (purchased from animal center of laboratory of Orchidaceae university, common grade) have a weight of 100 g-120 g.
1.2 detection of samples: ricinine (effective concentration 10%) obtained by the extraction method described in example 1 is taken, diluted and mixed uniformly with distilled water, and divided into 4 concentration groups of 25mg/kg, 50mg/kg, 100mg/kg and 200mg/kg according to 2.0 geometric progression.
1.3 test method: taking out the large part without abnormality after feeding (temperature 25 ℃ and humidity 55%) for one weekWhite mice were fasted for 12 hours before exposure to the toxin without water, body weight was measured, and then randomly divided into 4 groups of 10 animals (male and female halves). The stomach was irrigated once per oral, and the control group was irrigated with distilled water, according to 1.0mL/100g body weight. After administration, the presence or absence of toxic symptoms such as tremors, convulsions and dyspnea are observed, and the animal death number is recorded after continuous observation for 2 weeks. LD calculation according to modified koehne method 50 。
1.4 test results: the results of the acute oral toxicity test are shown in Table 4.
TABLE 4 acute oral toxicity test results of 10% ricinine mother liquor
The results showed that the high concentration group of mice showed toxic symptoms in the part of the mice about 2 hours after administration, which was marked by listlessness and Mao Pengsong. The poisoning symptoms are obvious after 24-72 hours of contamination, agitation, paroxysmal dyspnea and convulsion, and then the patient is listlessness and is not contracted until death. The control group rats did not develop toxic symptoms.
Rat acute oral LD of ricinine (effective concentration 10%) extracted by extraction method described in example 1 50 : female 76.96mg/kg; male 68.26mg/kg. The toxicity is moderate according to the pesticide toxicity grading standard in China. The raw materials used for preparing the preparation are described as safe to use.
2. Acute skin irritation test
2.1 test animals: new Zealand white rabbits (purchased from the laboratory animal center of Beijing) with a weight of 2 kg-3 kg.
2.2 test method:
1) 4 healthy white rabbits are selected and are subjected to experiment after being fed for 24 hours in an experimental environment (the temperature is 25 ℃ and the humidity is 55 percent);
2) Shearing two sides of the spine of each rabbit by 2X 6cm 2 ；
3) After 24 hours, 0.5mL of ricinine (the effective concentration is 10%) extracted by the extraction method described in the example 1 is coated on one side of the spine of each rabbit to remove hair skin, and then gauze is covered and fixed by adhesive tape, and the other side of the spine is coated with distilled water as a control;
4) After the medicine is applied for 4 hours, the medicine is washed by warm clear water and is gently wiped by gauze so as to remove the residual medicine on the skin;
5) And (3) observing and recording the formation of erythema and edema of the tested skin 1h, 24h and 72h after removing ricinine, and recovering the erythema and edema of the tested skin 7d and 14 d.
2.3 test results: the results of the acute skin irritation test are shown in Table 5.
TABLE 5 results of 10% ricinine mother liquor acute skin irritation test
Erythema formation: after ricinine is removed, the skin to be tested is not formed with erythema for 1-72 hours; edema formation conditions: the skin to be tested is not subjected to edema formation for 1-72 h after ricinine is removed. Skin of the control group was not abnormal. The raw materials used for preparing the preparation are described as safe to use.
3. Eye irritation test
3.1 test animals: new Zealand white rabbits (purchased from the laboratory animal center of Beijing) with a weight of 2 kg-3 kg.
3.2 test method:
1) 4 healthy white rabbits are selected and are subjected to experiment after being fed for 24 hours in an experimental environment (the temperature is 25 ℃ and the humidity is 55 percent);
2) Gently pulling down the eyelid on one side of each rabbit, dripping 0.1mL ricinine (effective concentration 10%) extracted by the extraction method described in example 1 into conjunctival sac, immediately gently closing the eyelid for 1min, and using distilled water for the other eye as control;
3) The stimulation response degree of cornea, iris and conjunctiva is observed at 1h, 24h, 48h and 72h after administration, and the response recovery condition is observed at 4d and 7 d;
4) The eyes are not washed within 24 hours after administration.
3.3 test results: the results of the eye irritation test are shown in table 6.
TABLE 6 eye irritation test results for 10% ricinine mother liquor
The conjunctival blood vessel of the tested animal is engorged with blood for 1-12 hours after the point medicine is taken, and the blood is bright red, and slight edema is accompanied with a small amount of secretion; no abnormalities were seen in iris and cornea. Congestion was still seen in the conjunctival vessels of the animals at 24h, and the animals were substantially restored to normal at 48 h. The eyes of the control group were normal. The raw materials used for preparing the preparation are described as safe to use.
As can be seen from the above examples, the invention provides the application of ricinine, a rodenticide, and the preparation method and application thereof, the ingestion coefficient of the rodenticide prepared by the invention is above 0.52, which is higher than the requirement that the A-level pharmacodynamic standard is not lower than 0.3, and the on-site test detection result shows that the rodenticide has a rodenticide rate of 95.17% in 21 days; the rodenticide provided by the invention is safe to use, store and transport, and has qualified stability.
The foregoing is merely a preferred embodiment of the present invention and it should be noted that modifications and adaptations to those skilled in the art may be made without departing from the principles of the present invention, which are intended to be comprehended within the scope of the present invention.
Claims (9)
1. The application of ricinine in preparing rodenticide.
2. The use according to claim 1, wherein the effective concentration of ricinine is 0.45-1.80%.
3. The rodenticide is characterized by comprising the following components in parts by weight: 0.45-1.80 parts of ricinine, 97-99 parts of base bait, 0.1-0.3 part of stabilizer and 0.002-0.02 part of bittering agent.
4. A rodenticide according to claim 3, wherein the base bait is one or more of corn flour, milk powder, peanut cake and vegetable oil; the stabilizer is dibutyl hydroxyl toluene; the bittering agent is benidialium.
5. A process for the preparation of a rodenticide according to claim 3 or 4, comprising the steps of:
(1) Mixing ricinine, base bait, stabilizer and bittering agent to obtain a mixture;
(2) Granulating the mixture, and drying to obtain the rodenticide.
6. The method of claim 5, wherein the method of drying is dryer heating; the temperature of the drying is 55-60 ℃.
7. The method according to claim 6, wherein the parameters in the granulation process are set as follows: the feeding speed is 10-20 kg/min, the rotating speed is 8-10 r/min, the discharging speed is 10-20 kg/min, and the pressure is 2-3 kg/cm 2 The temperature is 20-30 ℃.
8. Use of a rodenticide according to claim 3 or 4 for controlling rodenticides.
9. The application of the rodenticide prepared by the preparation method of any one of claims 5-7 in controlling rodenticide.
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Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1077840A (en) * | 1993-03-16 | 1993-11-03 | 大连市中山卫生技术服务部 | Lumpy deratization poison bait and preparation method thereof |
| CN1644057A (en) * | 2004-12-21 | 2005-07-27 | 仲恺农业技术学院 | Plant toxin for killing rats |
| CN101883492A (en) * | 2007-10-01 | 2010-11-10 | 巴斯夫欧洲公司 | Rodenticide mixture |
| CN111602678A (en) * | 2020-06-03 | 2020-09-01 | 丁柯中 | Biological rodenticide with multiple deratization effects and preparation method thereof |
| WO2022103263A1 (en) * | 2020-11-11 | 2022-05-19 | Metameta Research B.V. | Rodenticide |
-
2024
- 2024-02-04 CN CN202410152980.0A patent/CN117694341A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1077840A (en) * | 1993-03-16 | 1993-11-03 | 大连市中山卫生技术服务部 | Lumpy deratization poison bait and preparation method thereof |
| CN1644057A (en) * | 2004-12-21 | 2005-07-27 | 仲恺农业技术学院 | Plant toxin for killing rats |
| CN101883492A (en) * | 2007-10-01 | 2010-11-10 | 巴斯夫欧洲公司 | Rodenticide mixture |
| CN111602678A (en) * | 2020-06-03 | 2020-09-01 | 丁柯中 | Biological rodenticide with multiple deratization effects and preparation method thereof |
| WO2022103263A1 (en) * | 2020-11-11 | 2022-05-19 | Metameta Research B.V. | Rodenticide |
Non-Patent Citations (3)
| Title |
|---|
| 刘起勇 等: "《有害生物防治员国家职业标准远程培训教材（初、中级）》", 31 May 2021, 军事医学科学出版社, pages: 173 * |
| 郑雪莉 等: "小剂量蓖麻碱对小鼠毒性作用试验研究", 《西北林学院学报》, vol. 19, no. 4, 31 December 2004 (2004-12-31), pages 97 - 99 * |
| 高振川 等: "《猪饲料添加剂安全使用》", 31 December 2010, 金盾出版社, pages: 35 * |
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