CN117546802A - A large Daphnia locomotor behavior testing device - Google Patents

Abstract

The invention relates to a daphnia magna movement behavior testing device, which comprises a box body, wherein the box body is provided with a door body capable of being opened and closed, a turntable and a driving assembly for driving the turntable to rotate and position are arranged in the box body, a plurality of culture bottles capable of being taken and placed are arranged on the periphery of the upper surface of the turntable, a pretreatment chamber and a testing chamber which are close to each other are fixedly arranged on one side in the box body, the pretreatment chamber is surrounded by a light shielding plate, an inlet is arranged on one side wall of the testing chamber, an outlet is arranged on one side wall of the testing chamber, a communication port is arranged between the pretreatment chamber and the testing chamber, when the turntable rotates, each culture bottle sequentially passes through the inlet, the communication port and the outlet, a sensor assembly is arranged in the pretreatment chamber, a variable light source and a camera are fixedly arranged in the testing chamber, and the driving assembly, the sensor assembly, the variable light source and the camera are electrically connected with a controller. The integrated design has the advantages that the disturbance caused by conversion between parallel experiments is avoided; the integrated level is high and the throughput is high, and a plurality of groups of experimental tests can be completed by one-time setting, so that the test efficiency is greatly improved.

Description

Daphnia magna movement behavior testing device

Technical Field

The invention belongs to the field of daphnia magna movement behavior test, and particularly relates to a daphnia magna movement behavior test device.

Background

With the increased human activity, more and more chemicals are synthesized and used and enter the environmental medium, creating a potential threat to the ecosystem and human health. Implementing contaminant monitoring and understanding the multidimensional nature of chemical contaminants in a body of water is one of the important links to coping with chemical contamination. The daphnia magna is a zooplankton representative species which is most widely distributed and researched in a freshwater ecological system, so that the species is utilized to research the multidimensional toxicity and the water ecology suitability of pollutants, and the daphnia magna has the characteristics of uniformity of an experimental method, universality of experimental results and the like.

The daphnia magna kinematics study includes swimming time, speed, acceleration, vitality, jump frequency, swimming distance/track, steering frequency/angle, resting time, sinking rate, and movement behavior in the vertical direction of daphnia magna. Since the distribution of daphnia magna in a body of water and the locomotor behavior are usually closely related to the hydrophysics conditions to which they are adapted, such as illumination, temperature, salinity, water flow, etc. Therefore, the ecological environment of the water body can be monitored and evaluated according to the research result of the daphnia magna movement behavior. In the past, research of the effect of pollutants on the life history traits of daphnia magna is one of the most common and reliable assessment means. In recent years, however, research on the behavioral influence of pollutants on daphnia magna is more and more focused, and the research on the characteristics of life history is more in observation index and longer in flow; on the other hand, the response is quicker because the behavioural response trigger threshold is lower. Therefore, the research on the influence of pollutants on the behavior of daphnia magna is more suitable for rapid discrimination (applicable to both high and low concentrations).The behavior research index system of the daphnia magna developed at present comprises: swimming time, speed, acceleration, vigor, jump frequency, swimming distance/trajectory, steering frequency/angle, resting time, sinking rate, etc. The corresponding research means mainly comprise image recording and analysis, recording and/or analysis software and hardware applied to specific practice compriseAnd->Etc.

The method is a common video analysis software and is used for researching the movement behavior of daphnia magna. UsingThe software for researching the movement behavior of the daphnia magna needs to be subjected to six steps of data acquisition, video import, daphnia magna marking, movement tracking, movement track analysis, data export and analysis. The data acquisition firstly needs to record the motion trail of daphnia magna through imaging equipment (such as an underwater camera) to form a video material. Importing the acquired videoIn software, by->Marking means in the software mark daphnia magna, then +.>The software will track the movements of daphnia magna and record +.>A motion trail graph of daphnia magna can be generated. Finally, analysis using a software measurement toolThe movement speed, movement direction and other relevant parameters of the daphnia magna. The defects are that: the process of data acquisition and recording of the motion trail of daphnia magna is relatively complex, and the acquired video quality requirement is higher.

Daphnia Toximeter daphnia magna toxicity instrument is high-sensitivity toxicity detection equipment, which uses daphnia magna as a detection organism, detects the influence of a water sample on the quantity, moving speed, swimming height and circular frequency of daphnia magna, continuously detects the influence of a detected sample on the activity of daphnia magna by using a camera shooting and graph line analysis technology, and further determines the toxicity of the water sample. The device is used for observing and recording the reaction and behavior change of daphnia magna by exposing daphnia magna samples to a substance to be detected. The method comprises the steps of activity, feeding capacity, growth and development, reproductive capacity and other indexes of daphnia magna. By observing and analyzing these indexes, the toxicity level of the substance to be tested can be estimated. By using Daphnia Toximeter, potentially toxic substances in the environment, such as industrial waste water, pesticides, heavy metals, etc., can be detected to assess the environmental quality of the body of water and the health of the ecosystem. The defects are that: the method is mainly used for monitoring the reaction and behavior of daphnia magna under poison exposure, so as to analyze and judge the toxicity of the water sample, but is relatively weak in the aspect of daphnia magna motion trail imaging, the motion trail (especially the trail in the vertical direction) of daphnia magna cannot be intuitively displayed, meanwhile, the device structure is complex, the operation difficulty is high, and the high-flux and integrated test cannot be realized.

Disclosure of Invention

The invention provides a daphnia magna movement behavior testing device, which aims to overcome the problems in the prior art.

The technical scheme for solving the technical problems is as follows: the utility model provides a daphnia magna motion behavior testing arrangement, its includes the box, the box has the door body that can open and close, be equipped with carousel and drive in the box the drive assembly that the carousel rotated and was fixed a plurality of removable blake bottles of putting are equipped with to the periphery of carousel upper surface in circumference upper interval, still fixed pretreatment chamber and the test chamber that is equipped with each other in one side in the box, the pretreatment chamber is enclosed by the light screen and is equipped with the import on a lateral wall, be equipped with the export on the lateral wall of test chamber, be equipped with the intercommunication on the light screen between pretreatment chamber and the test chamber pretreatment chamber and the intercommunication mouth of test chamber, during carousel rotation, each the blake bottle can be passed through in proper order import, intercommunication mouth and export, be equipped with in the pretreatment chamber be used for right liquid in the blake bottle includes pH value and dissolved oxygen value's environmental parameter carries out automatic acquisition's sensor assembly, the fixed variable light source and the camera that is equipped with in the test chamber, drive assembly, sensor assembly, variable light source and all are connected with the controller electricity.

On the basis of the technical scheme, the invention can be improved as follows.

Further, the middle part level of box is fixed with the mounting panel, drive assembly includes brake motor and position sensor, the output of brake motor is vertical upwards to pass behind the centre bore of mounting panel with the center fixed connection of carousel that the level set up, brake motor with mounting panel fixed connection, position sensor and box fixed connection, the carousel in circumference interval be equipped with position sensor complex inductive head, inductive head triggers when position sensor, brake motor stall makes the carousel location.

Further, the box includes outer frame and locates the inner support in the outer frame, be fixed with on the outer frame and constitute the shielding plate of box wall, the inner support is located the mounting panel with between the box diapire in order to support fixedly the mounting panel.

Further, the temperature control assembly is used for regulating and controlling the temperature in the box body, and the temperature control assembly is arranged in the box body or arranged outside the box body and communicated with the box body.

Further, the camera is fixedly connected with the box body through a hanging rod, the camera is located above the middle area of the turntable and can shoot a culture bottle located in the testing chamber, and the variable light source is fixed on the lower surface of the top wall of the testing chamber and located between the camera and the culture bottle to be shot.

Further, a plurality of placing grooves used for placing and limiting the culture bottles are uniformly arranged on the periphery of the upper surface of the rotary table at intervals, two limiting convex columns are arranged at two ends of the bottom of each placing groove, outer extension edges are arranged at two ends of the bottom of the culture bottles, and limiting holes corresponding to the limiting convex columns are formed in the outer extension edges.

Further, the inlet and the communication port are both provided with self-resetting shading strips, when the culture bottle rotates along with the turntable, the self-resetting shading strips can be extruded to open the inlet and the communication port, and after the culture bottle is separated from the self-resetting shading strips, the self-resetting shading strips can automatically close the inlet and the communication port.

Further, the sensor assembly includes a pH sensor, a dissolved oxygen sensor, and an automatic elevation part, both of which are driven to be elevated by the automatic elevation part to be downwardly inserted into or upwardly separated from the culture flask in the pretreatment chamber.

Further, the box body is cuboid or square, the door body is arranged at the top of the box body, the rotary table is a circular table, and the area of the upper surface of the rotary table covered by the pretreatment chamber and the test chamber is 1/4 to 1/3 of the total area of the upper surface of the rotary table.

Further, be equipped with in the box with the power, drive assembly, sensor assembly, variable light source, camera and controller all with the power electricity is connected, the door body include two with the articulated door plant of box, be equipped with the handle on the door plant, two the door plant is split setting.

Compared with the prior art, the invention has the beneficial effects that:

through the integrated structural design, the device avoids disturbance caused by conversion between parallel experiments, and simultaneously can effectively avoid external interference; the device has high integration degree, and integrates pretreatment, experimental test and data analysis to a high degree; the device has the advantage of high flux, can complete a plurality of groups of experimental tests by one-time setting, and greatly improves the data collection efficiency; in addition, the device can provide better video imaging and track pictures for capturing the motion track of daphnia magna, and the whole operation of the device is simpler and more convenient.

Drawings

Fig. 1 is an isometric view of a daphnia magna movement behavior testing device provided by the invention;

FIG. 2 is an isometric view of the test device of FIG. 1 without the shielding plate on the wall of the housing;

FIG. 3 is a front view of the test device of FIG. 2;

FIG. 4 is a top view of the test device of FIG. 2;

FIG. 5 is an isometric view of the testing device of FIG. 1 without the door body, front wall, right wall, and inner and outer frames shown;

FIG. 6 is a top view of the test device of FIG. 5;

FIG. 7 is an isometric view of a shutter plate in the test apparatus of FIG. 5;

FIG. 8 is an isometric view of the structure of the camera, variable light source, etc. above the rotor of FIG. 5;

FIG. 9 is a rear elevational view of the structure illustrated in FIG. 8;

FIG. 10 is a top view of the structure shown in FIG. 8;

fig. 11 is an enlarged schematic view of the structure within the dashed circle a in fig. 8.

In the drawings, the list of components represented by the various numbers is as follows:

1. a case; 2. a door body; 3. a turntable; 4. a culture bottle; 5. a light shielding plate; 6. a pretreatment chamber; 7. a test chamber; 8. an inlet; 9. an outlet; 10. a communication port; 11. a variable light source; 12. a camera; 13. a controller; 14. a mounting plate; 15. a brake motor; 16. a position sensor; 17. an induction head; 18. an outer frame; 19. an inner bracket; 20. a placement groove; 21. limiting convex columns; 22. an outer extension edge; 23. a limiting hole; 24. a power supply; 25. a handle.

Detailed Description

The principles and features of the present invention are described below with reference to the drawings, the examples are illustrated for the purpose of illustrating the invention and are not to be construed as limiting the scope of the invention.

In the description of the present invention, if terms indicating orientations such as "upper", "lower", "left", "right", "top", "bottom", "inner", "outer", etc. are used, the indicated orientations or positional relationships are based on the orientations or positional relationships shown in the drawings, only for convenience in describing the present invention and simplifying the description, and do not indicate or imply that the indicated devices or elements must have a specific orientation, be configured and operated in the specific orientation, and thus are not to be construed as limiting the present invention.

As shown in fig. 1 to 11, the invention provides a device for testing daphnia operation, which comprises a box body 1, wherein the box body 1 is provided with a door body 2 which can be opened and closed, a rotary table 3 and a driving component which drives the rotary table 3 to rotate and position are arranged in the box body 1, a plurality of culture bottles 4 which can be taken and placed are arranged at intervals on the periphery of the upper surface of the rotary table 3 in the circumferential direction, a pretreatment chamber 6 and a testing chamber 7 which are close to each other are fixedly arranged on one side in the box body 1, the pretreatment chamber 6 is surrounded by a shading plate 5, an inlet 8 is arranged on one side wall of the pretreatment chamber 6, an outlet 9 is arranged on one side wall of the testing chamber 7, a communication port 10 which is communicated with the pretreatment chamber 6 and the testing chamber 7 is arranged on the shading plate 5, when the rotary table 3 rotates, each culture bottle 4 sequentially passes through the inlet 8, the communication port 10 and the outlet 9, a sensor component which is used for carrying out the acquisition of environmental parameters including a pH value and an automatic dissolved oxygen value on liquid in the culture bottle 4 is arranged in the pretreatment chamber 6, a sensor component which is fixedly connected with a camera 11, a sensor component which is fixedly connected with a sensor component 11, and a variable light source 11. The variable light source 10 selects an LED lamp that can vary the color of the lamp light.

When the door body is opened, each culture bottle filled with the culture solution and the large daphnia can be manually placed at the corresponding position around the turntable (the culture solution containing corresponding components is prepared in advance according to the experiment or test requirement or the liquid and the large daphnia are directly put into the culture bottle for testing when unknown liquid is tested), then the door body is closed, the testing device is started, the controller controls the driving assembly, the sensor assembly, the variable light source, the camera and the like to operate according to the set program, each culture bottle rotates and is positioned along with the turntable, each culture bottle can enter the pretreatment chamber from the inlet for dark adaptation and environmental parameter acquisition (pH value, dissolved oxygen value and the like) in the whole testing process, then enters the testing chamber from the communication port for observation and recording of movement behaviors through the camera, and finally exits from the testing chamber from the outlet. The test is continuous and gradual, and when the daphnia magna in the culture flask is observed in the test room, the daphnia magna in a plurality of culture flasks is subjected to dark adaptation and/or environmental parameter acquisition in the pretreatment room.

It can be understood that daphnia magna is a type of zooplankton, and the device provided by the invention can be used for observing and testing other zooplankton, and daphnia magna can be widely understood as a representative of the type of zooplankton.

In one embodiment of the present invention, as shown in fig. 2, 5 and 8, a mounting plate 14 is horizontally fixed at the middle of the case 1, the driving assembly includes a brake motor 15 and a position sensor 16, an output end of the brake motor 15 vertically passes through a central hole of the mounting plate 14 upwards and is fixedly connected with the center of the horizontally arranged turntable 3, the brake motor 15 is fixedly connected with the mounting plate 14, the position sensor 16 is fixedly connected with the case 1, the turntable 3 is circumferentially provided with induction heads 17 matched with the position sensor 16 at intervals, and when the induction heads 17 trigger the position sensor 16, the brake motor 15 stops transmitting to position the turntable 3.

It should be noted that, the position sensor may be a metal proximity switch, the whole rotating disc or at least one circle of outer edge of the rotating disc may be made of non-metal material, and a plurality of induction heads (such as screws) made of metal material are uniformly spaced on the outer edge cylindrical surface of the rotating disc, each induction head corresponds to a position of a culture bottle on the rotating disc, when the rotating disc rotates, the induction heads are made to approach the position sensor and are instantly triggered to the regular position sensor, the position sensor gives a signal to the pre-controller, the controller immediately controls the brake motor to stop rotating, then the rotating disc stops rotating and the culture bottle on the rotating disc has 1 place in the testing chamber, and 2-3 or more culture bottle placing slots are arranged in the pre-processing chamber (preferably, as shown in fig. 10, there are three pre-processing stations, one detection station), when the controller receives the signal of the position sensor, besides controlling the motor to stop rotating, the camera is synchronously started to observe and shoot, the variable light source is controlled by the controller to start or adjust different frequencies of light to the culture bottles in the testing chamber according to the test requirements, the controller is started up for a time period of setting up again for the test wheel when the controller starts up the test wheel to observe the next time for the rotation of the test motor.

In one embodiment of the present invention, the case 1 includes an outer frame 18 and an inner bracket 19 disposed in the outer frame 18, a shielding plate forming a wall of the case 1 is fixed on the outer frame 18, and the inner bracket 19 is disposed between the mounting plate 14 and a bottom wall of the case 1 to support and fix the mounting plate 14.

The outer frame and the inner bracket are preferably aluminum profiles or aluminum alloy profiles, the shielding plate is preferably a light-tight plastic plate, a thin steel plate and the like, and the shielding plate and the outer frame are fixed through screws or rivets. The inner bracket is not only used for supporting the mounting plate so as to bear the weight after the motor is mounted, but also comprises a plurality of struts extending from the outer frame into the box, and the struts are used for supporting and fixing a light shielding plate forming the pretreatment chamber and the testing chamber, and are used for fixing a camera or a variable light source.

In one embodiment of the present invention, the temperature control device further comprises a temperature control component for controlling the temperature in the box body 1, wherein the temperature control component is arranged in the box body 1 or arranged outside the box body 1 and is communicated with the inside of the box body 1.

It can be understood that the temperature control component is an existing structure (such as a common thermostat or an air conditioning component) and can regulate and control and maintain the temperature in the box body so as to meet the requirement of a tester for observing and recording the movement behaviors of the daphnia magna at different temperatures. The temperature control assembly includes temperature sensors, heating and/or cooling components, the specific structure, installation and control of which are well known to those skilled in the art and will not be described in detail herein.

In one embodiment of the present invention, as shown in fig. 6, 8 and 9, the camera 12 is fixedly connected to the case 1 through a hanging rod (for example, connected to a supporting rod extending downwards from the top of the outer frame), the camera 12 is located above the middle area of the turntable 3 and can shoot the culture flask 4 located in the test chamber 7, and the variable light source 11 is fixed to the lower surface of the top wall of the test chamber 7 and is located between the camera 12 and the culture flask 4 to be shot.

It can be understood that, in order to more clearly shoot the movement behavior of the daphnia magna, the camera can be provided with a position adjusting power component, the power component is connected with the controller, and the position of the camera can be automatically controlled and adjusted by the controller or manually adjusted by the controller to more clearly and accurately shoot the movement of the daphnia magna.

In one embodiment of the present invention, as shown in fig. 11, a plurality of placement grooves 20 for placing and limiting the culture bottles 4 are uniformly arranged at intervals on the periphery of the upper surface of the turntable 3, two limiting convex columns 21 are respectively arranged at two ends of the bottom of each placement groove 20, two outer extension edges 22 are arranged at two ends of the bottom of the culture bottle 4, and limiting holes 23 corresponding to the limiting convex columns 21 are arranged on the outer extension edges 22.

It should be noted that, after spacing protruding insert the spacing hole on the outer edge of culture flask (cell) bottom both sides, can not produce the displacement when the culture flask receives the effort of horizontal direction, reaches the purpose of stable placing, when the culture flask need be taken off, the manual work upwards pulls the culture flask from the door body department that the box top was opened, can make culture flask and spacing projection disengage.

In one embodiment of the present invention, the inlet 8 and the communication port 10 are provided with self-resetting light shielding strips, when the culture flask 4 rotates along with the turntable 3, the self-resetting light shielding strips can be extruded to open the inlet 8 and the communication port 10, and after the culture flask 4 is separated from the self-resetting light shielding strips, the self-resetting light shielding strips can automatically close the inlet 8 and the communication port 10.

It can be understood that in order to ensure a better dark environment in the pretreatment chamber so as to carry out dark adaptation pretreatment on the daphnia magna in the culture flask entering the pretreatment chamber, the inlet and the communication port of the pretreatment chamber are preferably closed and shielded when no culture flask passes, so that the self-resetting shielding strip is arranged to achieve the aim, when the culture flask passes, the self-resetting shielding strip is pressed to open the inlet (the communication port) or is opened through a power mechanism which is additionally arranged, and when the culture flask breaks away from, the inlet and the communication port are automatically closed.

In one embodiment of the present invention, the sensor assembly includes a pH sensor, a dissolved oxygen sensor, and an automatic elevation part, both of which are elevated by the automatic elevation part to be inserted downward or to be moved upward away from the culture flask 4 in the pretreatment chamber 6.

The automatic lifting part can automatically lift and finish the collection of the environmental parameters including the pH value, the dissolved oxygen value and the like of the liquid in the culture bottle only when the culture bottle is aligned below the automatic lifting part. The automatic lifting part can be linked with the position sensor, when the position sensor is triggered, the automatic lifting part is triggered and performs the actions of descending first and then collecting data according to a set program, and then ascending and resetting.

In one embodiment of the present invention, the box 1 is cuboid or square, the door 2 is arranged at the top of the box 1, the turntable 3 is a circular disk, and the pretreatment chamber 6 and the test chamber 7 cover the upper surface of the turntable 3 in an area of 1/4 to 1/3 of the total upper surface area of the turntable 3.

It should be noted that, above-mentioned structural design is favorable to whole testing arrangement's miniaturization, can realize that a plurality of blake bottles are parallel to be tested under the same environment, avoids the device volume too big and occupy great space again.

In one embodiment of the present invention, the power source 24 is disposed in the case 1, the driving assembly, the sensor assembly, the variable light source 11, the camera 12 and the controller 13 are all electrically connected to the power source 24, the door 2 includes two door panels hinged to the case 1, the door panels are provided with handles 25, and the two door panels are disposed in a split manner.

It can be understood that the parts of the whole device which need electric driving can be powered by a power supply, the power supply can be a lithium battery which can be charged and discharged repeatedly, and besides the form with the built-in power supply, the testing device provided by the invention can also directly use the commercial power as a driving power supply.

The foregoing description of the preferred embodiments of the invention is not intended to limit the invention to the precise form disclosed, and any such modifications, equivalents, and alternatives falling within the spirit and scope of the invention are intended to be included within the scope of the invention.

Claims (10)

1. The utility model provides a large daphnia motion behavior testing device, its characterized in that, including box (1), box (1) has door body (2) that can open and close, be equipped with carousel (3) and drive in box (1) carousel (3) rotation and the drive assembly of location, the periphery of carousel (3) upper surface is equipped with a plurality of removable blake bottles (4) of putting in circumference upper interval, box (1) inboard still is fixed and is equipped with pretreatment chamber (6) and test chamber (7) next to each other, pretreatment chamber (6) are enclosed by light screen (5) and are equipped with import (8) on a lateral wall, be equipped with export (9) on a lateral wall of test chamber (7), be equipped with on light screen (5) between pretreatment chamber (6) and test chamber (7) and intercommunication in advance pretreatment chamber (6) and test chamber (7), when carousel (3) rotate, each blake bottle (4) all can pass through in proper order import (8), intercommunication mouth (10) and export (9) are equipped with import (8) on a lateral wall, be equipped with on a lateral wall of test chamber (7) and one side of being equipped with import (8), sensor group (7) can be used for carrying out in-house (12) the internal-phase change sensor system oxygen sensor (12) to dissolve in the environment (11), the driving assembly, the sensor assembly, the variable light source (11) and the camera (12) are all electrically connected with the controller (13).

2. The daphnia magna movement behavior testing device according to claim 1, characterized in that the middle part of the box body (1) is horizontally fixed with a mounting plate (14), the driving assembly comprises a brake motor (15) and a position sensor (16), the output end of the brake motor (15) vertically passes through a central hole of the mounting plate (14) upwards and then is fixedly connected with the center of the horizontally arranged turntable (3), the brake motor (15) is fixedly connected with the mounting plate (14), the position sensor (16) is fixedly connected with the box body (1), the turntable (3) is circumferentially provided with an induction head (17) matched with the position sensor (16) at intervals, and when the induction head (17) triggers the position sensor (16), the brake motor (15) stops rotating to enable the turntable (3) to be positioned.

3. The daphnia sport behavior testing device according to claim 2, wherein the box body (1) comprises an outer frame (18) and an inner bracket (19) arranged in the outer frame (18), a shielding plate forming the box wall of the box body (1) is fixed on the outer frame (18), and the inner bracket (19) is arranged between the mounting plate (14) and the bottom wall of the box body (1) to support and fix the mounting plate (14).

4. The daphnia sport behavior testing device according to claim 1, further comprising a temperature control assembly for regulating and controlling the temperature in the box body (1), wherein the temperature control assembly is arranged in the box body (1) or arranged outside the box body (1) and communicated with the inside of the box body (1).

5. The daphnia magna sport behavior testing device according to claim 1, wherein the camera (12) is fixedly connected with the box body (1) through a hanging rod, the camera (12) is located above the middle area of the turntable (3) and can shoot a culture bottle (4) located in the testing chamber (7), and the variable light source (11) is fixed on the lower surface of the top wall of the testing chamber (7) and located between the camera (12) and the culture bottle (4) to be shot.

6. The daphnia magna sport behavior testing device according to claim 1, wherein a plurality of placing grooves (20) used for placing and limiting the culture bottles (4) are uniformly arranged on the periphery of the upper surface of the rotary table (3) at intervals, two limiting convex columns (21) are arranged at two ends of the bottom of each placing groove (20), outer extension edges (22) are arranged at two ends of the bottom of the culture bottles (4), and limiting holes (23) corresponding to the limiting convex columns (21) are formed in the outer extension edges (22).

7. The daphnia magna sport behavior testing device according to claim 1, wherein the inlet (8) and the communication port (10) are both provided with self-resetting shading strips, when the culture flask (4) rotates along with the rotary table (3), the self-resetting shading strips can be extruded to open the inlet (8) and the communication port (10), and after the culture flask (4) is separated from the self-resetting shading strips, the self-resetting shading strips can automatically close the inlet (8) and the communication port (10).

8. The daphnia magna sport behavior testing device according to claim 1, wherein the sensor assembly comprises a pH sensor, a dissolved oxygen sensor and an automatic lifting part, and the pH sensor and the dissolved oxygen sensor are driven by the automatic lifting part to lift so as to be inserted downwards or upwards away from the culture flask (4) in the pretreatment chamber (6).

9. The daphnia magna sport behavior testing device according to claim 1, wherein the box body (1) is cuboid or square, the door body (2) is arranged at the top of the box body (1), the rotary table (3) is a circular plate, and the area covering the upper surface of the rotary table (3) is 1/4 to 1/3 of the total surface area of the upper surface of the rotary table (3) covered by the pretreatment chamber (6) and the testing chamber (7).

10. The daphnia sport behavior testing device according to any one of claims 1 to 9, wherein a power supply (24) is arranged in the box body (1), the driving component, the sensor component, the variable light source (11), the camera (12) and the controller (13) are all electrically connected with the power supply (24), the door body (2) comprises two door plates hinged with the box body (1), a handle (25) is arranged on each door plate, and the two door plates are arranged in a split mode.