CN208283346U - Thin-layer chromatographic analysis automatic intelligent detecting instrument - Google Patents
Thin-layer chromatographic analysis automatic intelligent detecting instrument Download PDFInfo
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- CN208283346U CN208283346U CN201820516945.2U CN201820516945U CN208283346U CN 208283346 U CN208283346 U CN 208283346U CN 201820516945 U CN201820516945 U CN 201820516945U CN 208283346 U CN208283346 U CN 208283346U
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Abstract
The utility model discloses thin-layer chromatographic analysis automatic intelligent detecting instrument, including chromatographic sheet box, expansion and colour developing liquid phase groove box, the rotation drive device of mobile chromatographic sheet, infusion pump solvent injection device, infusion pump sample sample spot sample device, dry and image capture apparatus.The utility model can automatically control sampling in thin-layer chromatographic analysis, point sample, expansion, colour developing, scanning, and real-time online carries out the qualitative or quantitative analysis of sample.The utility model energy real-time tracking sample composition changes with time and chemical reaction course, saves manpower, improves efficiency, and reduces human error.
Description
Technical field
The utility model relates to the chemical-biological sample separate analytical techniques of inorganic, organic compound or large biological molecule etc.
Field, and in particular to thin-layer chromatographic analysis automatic intelligent detecting instrument.
Background technique
At the beginning of red, orange, green, blue, yellow (ROGBY) (Chromatography) originates in eighties of last century, dosage is generally several to several hectogammas, is
A kind of more practical, effective microseparation analysis method.The most common liquid chromatography has thin-layered chromatography and column chromatography.It is former
Reason is the difference according to adsorbent of the same race to the adsorption capacity of various sample compositions, so that mobile phase (solvent) is flowed through solid phase and (inhales
Attached dose) during, separate the experimental technique of various composition.Common adsorbent has silica gel, aluminium oxide etc..Mobile phase solvent one
As have the various organic solvents such as water, methanol, ethyl alcohol, methylene chloride, ethyl acetate or many of solvent by a certain percentage
Mixed solution.Column chromatography is to insert absorbent particles in the pillar of glass or other materials to separate sample with solvent washing
Method.Can with the efficient liquid phase of high pressure washing chromatographic column (High Performance Liquid Chromatography,
HPLC) chromatographic to develop the resolution ratio for improving sample composition, so that column layer method is become most common chemical or biological sample
Method for separating and analyzing.
Thin-layered chromatography is that adsorbent is applied on chromatographic sheet, then adds sample on the sorbent, and solvent is logical
Cross that capillary phenomenon is unfolded on chromatographic sheet and sample composition is separated on chromatographic sheet.High performance thin layer chromatography method
The development of (High Performance Thin Layer Chromatography, HPTLC) can be used as in chemical analysis
Play increasingly consequence.The combination of thin-layered chromatography and other spectroscopic methodologies, the spectrodensitometry of digital camera, biology are anti-
It should shine, the exploitation of nano adsorption layer becomes better and better to the separating effect and detection accuracy of sample.In recent years to being applied in developing tank
Add external add-in power such as electromagnetic field, high pressure etc. and generate electrophoresis thin-layer chromatography (Pressurized Plannar
Electrochromatography PPEC), high pressure thin-layer chromatography (Overpressured Layer Chromatography
The methods of) OPLC the detection resolution of sample is also improved.(referring to Colin F.Poole, Instrumental Thin-
Layer Chromatography.Elsevier 2015)
Although in resolution ratio, thin-layered chromatography not as good as high performance liquid chromatography, thin-layered chromatography can be thin at one piece
Multiple samples are measured in parallel on layer chromatography plate, analysis speed is fast, and it is easy to operate simple, and can intuitively use image recording point
Analysis.In medicine, the fields such as pharmacy, biochemistry, health, environment, food, chemical industry are widely used.Thin-layer chromatography is frequently used for medium-height grass
Quality management and the native compound of medicine production and identification, identification, determination of foreign matter or the assay of chemicals.In drug
In research and development, thin layer chromatography is used to measure quantitative structure activity relationship (the Quantitative Structure of drug development
Activity Relationship, QSAR) the physical-chemical parameters (logP).In addition, thin-layer chromatography bioautography method
(TLC-Bioautography) it is applied to antibacterium, antimycotic drug screening.(referring to K.Ciura et al, Journal
of Chromatography A,1520(2017)9-22.)
In chemosynthesis reaction detection, initial compounds or generation of the thin-layered chromatography to chemical reaction are usually utilized
Compound is detected with the changes of contents in reaction time, to learn the process of chemosynthesis reaction.
The specific step of thin layer chromatography be sampling, point sample, expansion, drying volatilize sample or developing solvent, colour developing
The qualitative analysis or progress UV scanning observed under agent colour developing, ultraviolet lamp calculate the quantitative analysis of sample size.
Point sample: most classic method is sampled with glass capillary, by sample solution point to the chromatographic sheet prepared
On one end, until sample is fixed on the silica gel particle on chromatographic sheet.Solvent is volatilized completely with cool breeze and hot wind, energy
Preferably control arraying quality.
Expansion: chromatographic sheet one end with sample is put into the solvent of developing tank, sample point will be in solvent circle
On face, solvent can move expansion on chromatographic sheet, due to the adsorption capacity of each ingredient of sample it is different and in thin-layer chromatography
The Rf value (Rf) being unfolded on plate is different, achievees the purpose that separation.Rf value (Retardation factor, Rf) refers to and is opening up
In open procedure, the ratio between moving distance and mobile phase solvent moving distance of sample composition can be expressed with following formula:
Rf=Zx/ (Zf-Z0)
Wherein:
Zx, solvent front is at a distance from origin (at point sample)
Zf, solvent front is at a distance from mobile phase junction
Z0, origin is at a distance from chromatographic sheet and mobile phase junction
When with common chromatographic sheet, Rf value is related with its polarity size.The small Rf value of polarity is larger, and polarity is big
Rf value is smaller.
Colour developing: after chromatographic sheet expansion, chromatographic sheet is put into color developing agent or with color developing agent to chromatographic sheet
Be sprayed, the sample being adsorbed on chromatographic sheet made to react with color developing agent, under room temperature or heating condition sample at
Dividing can be absorbed visible light at expansion and color is presented.It, can be in chromatographic sheet adsorbent to the sample for having UV absorption
In fluorescence indicator, such as silica gel plate GF254 is added, generate uniform fluorescence background under the excitation of ultraviolet light, and sample spot
Point is then shown with dead color, and can be photographed to record.
Qualitative analysis: in the coloured situation of sample, the expansion separating resulting of sample can be directly observed with eyes.If
Sample is colourless but has UV absorption, can use fluorescent thin-layer chromatography plate and observes and records separating resulting in the UV lamp.In addition may be used
Judged with using color developing agent that the chromatographic sheet after expansion develops the color.
Quantitative analysis: the sample composition after usually quantifying chromatographic sheet expansion with thin layer chromatography scanning.Thin layer scanning is
With the sample point on visible light or ultraviolet light chromatographic sheet, absorbance (A) travelling with the exhibits on an exhibition tour of thin-layer chromatography spot is measured
The variation for opening distance obtains tlc scanning curve.Quantifying for thin layer chromatography scanning is based on Gu Charles Bell Ka-Man Ke
(kubelka-Munk) law.Ancient Charles Bell Ka-Man Ke law illustrates the diffusion parameter of stationary phase to sample component in spot
The influence of concentration and absorbance relationship.
Ancient Charles Bell Ka-Man Ke (kubelka-Munk) law theoretically can express (reference with following mathematical formulae
Spangenberg B.J.Planner Chromatography 2006；19；332):
Wherein:
R ∞, the absolute reflectance of infinite thickness adsorption layer
ε, molar absorption coefficient
S, the coefficient of dispersion
C, the spot concentration of thin-layer chromatography
N, mole sample size
A, speck area
D, adsorbent layer thickness
A, absorption coefficient
More accurate spectrographic technique is usually required to the sample amounts after expansion separation.On chromatographic sheet most often at present
Measuring method is multiband and diode matrix scanning and the measurement with digital camera to optical density.Quantitative approach, which has, returns
One method, external standard method, additional method and regression curve sizing technique.More commonly used is regression curve method, by standard solution various concentration
With sample solution point on same chromatographic sheet, thin layer scanning is carried out after expansion, obtains the regression curve of standard solution, and
Sample size is calculated with regression curve afterwards.Meanwhile thin-layer chromatography can also be combined with other spectroscopic methodologies such as mass spectrum, infrared and nuclear-magnetism
Resonance carries out more accurate identification and quantification to analysis sample.
In actual operation, thin layer chromatography test sample is required through point sample, and solvent expansion separation has
Chemical colour reaction step is needed, it is visual finally by human body eye, or scanned using spectrometer qualitative or quantitative to chemistry and raw
Object sample composition is analyzed.Each step will laboratory technician according to experiment flow step, step by step take action on one's own to operate.
Firstly, will on chromatographic sheet point sample, i.e., by sample solution with capillary glass tube drop on chromatographic sheet, wait point samples complete
Afterwards, chromatographic sheet is placed into developing tank and carries out solvent expansion.In this course, sample composition can be because of ingredient to chromatography
The adsorption capacity difference of chromatographic sheet adsorbent is separated.To it is after expansion as a result, if sample be it is colourless, need to utilize
Chemochromic reagent colour developing, i.e., taking-up after sample chromatographic sheet being put into developing solution, on hot plate heating colour developing.If
Sample composition has UV absorption, can save chemical colour reaction step, and irradiates lower observation with ultraviolet light, is remembered with hand or instrument
Record separating resulting.
What thin-layer chromatographic analysis operation at present substantially carried out in open environment.Outer bound pair by separation sample effects very
, the influence of solvent vapour, the influence and exhibition of temperature away from influence etc..Thin-layer chromatographic analysis
Overall process be offline many more manipulations, operating process and skill will affect the quality of chromatography.Thin-layer chromatographic analysis usually requires reality
The person of testing waits under lab, goes to complete these steps according to the experiment progress time, can consume laboratory technician's a large amount of time.Together
Sample, when detecting chemical reaction course, full laboratory technician's manual operations thin-layer chromatographic analysis, and need with chemical reaction into
Journey in real time detects chemical reactant and product, and the thin of multiple chemical reactions cannot be carried out simultaneously by limiting laboratory technician
Layer chromatography detection operation, in addition, the thin-layer chromatographic analysis of Qualitative thin-layer's chromatography especially chemosynthesis reaction is only with master
Record tlc analysis is seen as a result, without original objective record, quality management and the standardization of data cannot be preferably carried out and answer
With.
Although each step of thin-layered chromatography is by instrumentation, such as point sample instrument, developing instrument, multiple developing instrument, atomization colour developing
Instrument, UV scanning and integrator etc. have certain improvement to nonstandard operation and efficiency, still, in actual analysis operating process, this
A little instruments are usually the accurate processing for being directed to the chromatographic sheet of a step and the quantitative analysis carried out, and each step will be from
Line operation is completed.Point sample on chromatographic sheet, solvent expansion, dry, chemical colour reaction or UV scanning will be manually by thin layers
Chromatosheet is put into the instrument of each step, and operations is waited to complete, and whole process will be accompanied manually, need to use up operator
The extensive work time.Moreover, each step instrument is all typically used for handling larger lamellae (such as 10x20 centimetres or 20x20 lis
Rice) chromatography or more expensive or bulky and be all off-line operation, limit these instruments for thin-layer chromatography
Analyze the purposes for the simple qualitative comparison being most good at.
It is most efficient method by the on-line analysis detection of each step operation full-automation.Due to efficient liquid phase (HPLC)
The chromatographic column of chromatography can repeatedly be used for sample analysis and not have to replacement, and the sampling in operation, sample introduction divides into chromatographic column
It is easier full-automation from detection, the in-line analyzer of efficient liquid phase (HPLC) is begun to early in the eighties in last century
Device.Compared with the efficient liquid phase (HPLC) of column layer chromatography, the lamellae of thin-layered chromatography can typically be only used to one after point sample
Secondary sample analysis, the sample of opposed polarity needs different developing solvents, therefore thin-layer chromatography detection and analysis need to be replaced frequently
Chromatographic sheet and developing solvent, all the automation of analysis operation is opposite is difficult.With the on-line analysis of efficient liquid phase (HPLC)
It has been that the situation of normality is compared, thin-layered chromatography (TLC) lacks effective real-time on-line detecting method.
Moreover, it is noted that many analysis methods are also to be coated with a stratification on thin plate as thin-layered chromatography
Learn substance and separate purpose in order to which with this layer of chemical substance physically or chemically effect arrival occurs for sample, then to after separation at
Divide and carries out qualitative spectrometric or quantitative analysis.It is main have be usually used in analyze protein and the electrophoresis analytical method of DNA etc..These points
Analysis method operating process having the same, i.e. sampling, point sample, dyeing, image scanning, there is also off-line operation etc. is identical
The problem of.
Utility model content
To be solved in the utility model is the inefficient of existing thin layer chromatography, nonstandard artificial off-line operation
More, poor reproducibility and quality problem difficult to control are, and it is an object of the present invention to provide thin-layer chromatographic analysis automatic intelligent detecting instrument, by mesh
Sampling, point sample, expansion, colour developing, drying in preceding chromatographic sheet analytic approach, scanning or image capture record and quantitative calculating are more
A step or more instruments, complete and realize the thin layer of real-time online with an automatic detection instrument of the utility model
Chromatography determination method.
The automation thin-layer chromatography detection device that the real-time on-line detecting method of the utility model is utilized includes rack, storage
The chromatographic sheet box of chromatographic sheet is deposited, the liquid phase groove box of sample expansion supports the sample application platform of chromatographic sheet, mobile
The rotation drive device of chromatographic sheet, to the spot sample device that point sample position and point sample amount are accurately controlled, and to thin layer
The equipment and automatic control system of chromatosheet progress image capture and analysis.
What the solvent expansion slot device of the thin-layer chromatography detection intelligence instrument of the utility model usually carried out is longitudinally perpendicular
Development of Thin-Layer Chromatography mode, the liquid phase groove as expansion are made of the rectangular channel of side opening, and multiple liquid phase grooves are vertically folded
Add and constitutes liquid phase groove box.The bottom of liquid phase groove is connect with infusion pump, and infusion pump provides the injection and extraction of developing solvent.Infusion pump
Including but not limited to vacuum pump, peristaltic pump, reciprocating pump etc..
According to the specific analytical method of thin-layer chromatography, the mountable application electric field of liquid phase groove, electromagnetism, high-pressure installation carry out institute
The chromatography needed required device such as in electrophoresis tlc analysis (PPEC), the methods of high pressure tlc analysis (OPLC).
The liquid in-out mouth of infusion pump can be assembled in the appropriate location of liquid phase cell wall, accurately control liquid inlet volume, carry out gradient, expand horizontally
And 2D expansion (2dimensional TLC development).
The sample spot sample device of the utility model can be fixed in closed rack, by infusion pump, horizontal slide unit, longitudinal direction
Slide unit and sampling head are constituted.Horizontal slide unit and longitudinal slide unit are used to position the position of sampling head, sampling head and an infusion pump
Connection can more accurate control points sample amount raising point sample precision.In addition, the liquid feeding end of infusion pump connects sample solution, can periodically take out
Sample solution is taken to carry out point sample.Such a sampling infusion pump can be combined with crossover valve, while to the sample in multiple containers
Solution carries out parallel real-time online sample detection.For example, the infusion pump of sampler can be connect with multiple chemical reaction instruments
And real-time online detect multiple chemical reactants or product content and principal reaction process.
The image capture analytical equipment of the utility model, which has, carries out Image Acquisition to the chromatographic sheet after sample expansion
Equipment is including but not limited to ultraviolet, infrared scanner or industrial camera, ultraviolet lamp, fluorescent lamp, heating or blowing-dryer.Image
Capturing apparatus is typically mounted on the top of chromatographic sheet sample surface position, after solvent expansion, by UV scanning, ultraviolet photograph
It penetrates the operations such as take pictures or take pictures after developing the color and carries out image capture and record.
The utility model provide one simply and effectively automation rotation drive device by chromatographic sheet from thin layer color
Sample application platform is skidded off in spectrum plate box and carries out point sample, is then slided into, is skidded off liquid phase groove, is able to achieve one by one from chromatographic sheet box
It takes out one or more chromatographic sheets and carries out chromatography operation.Both ends in this rotation drive device possess thin layer color
Plate box and liquid phase groove box are composed, rotation drive device center has support and mobile chromatographic sheet sample application platform channel.It adjusts thin
The rotation direction of the position and rotation drive device of layer chromatography plate box and liquid phase groove box and sample application platform, can be by thin-layer chromatography
Plate, which is moved to above sample application platform and carries out point sample, moves into developing tank while injecting developping solution, carries out solvent expansion, then by thin layer
The top that chromatosheet is moved out to plate box, liquid phase groove box or sample application platform carries out the operation of multiple steps such as image capture.It is all this
A little thin-layer chromatographys, which test and analyze operation and controlled by automation control process, makes it possible on-line analysis.
The automatic control system of the utility model may include the control software and/or off line PLC control system of PC computer sum
To execute control mechanical movement instruction and sampling point sample operational order.PC computer has Image Acquisition and quantitative analysis soft simultaneously
Part.Quantitative analysis software can use regression curve quantitative approach, acquires the regression curve of standard sample and calculates recurrence song
Line formula carrys out the content of Real_time quantitative detection unknown sample.
The rotation driving that the utility model can combine multiple chromatographic sheet boxes, liquid phase groove box and institute's utility model fills
It sets, is able to achieve analysis hundreds of sample within the One step development period, realize that high-throughput diversified expansion mode is such as more
Secondary expansion, high pressure expansion, electromagnetism expansion etc. and improve chromatography quality and efficiency.
The utility model can be used for the fields such as medicine, pharmacy, biochemistry, health, environment, food, chemical industry it is high-throughput with
And real-time online thin-layer chromatographic analysis.For example, the utility model detecting instrument can be used to identify sample in Chinese herbal medicine production process
The real-time online component content of product detects to carry out quality management, and the multiple chemical reactions of detection can be automatically tracked with real-time online
Progress identifies the reaction terminating time by the quantitative detection to reactant or production object, so that it is anti-to automatically control chemistry synthesis
It answers.The utility model detecting instrument, which saves most of manual operation, can be used for dangerous work environment, such as have severe toxicity, strength
The real-time online sample composition of irritant gas, reflective substance etc. detects.
The utility model can be used for preparation of lamina chromatography.Since every piece of chromatographic sheet is all from rotation drive device
Recovery approach recycling, by acquisition expansion after recycle chromatographic sheet on adsorbent, with solvent extraction obtain separation sample at
Point.
In addition to the real-time detection method for thin layer chromatography, the utility model can be used for making using thin plate
For the analysis method of sample carrier, such as electrophoresis analytical method and cell dyeing analysis.Utilize plate box of the invention, liquid phase groove box
These analysis methods can be become to the automatic of real-time online with rotation drive device and corresponding image capture analytical equipment
Change determination method.
The utility model is realized by following technical operation schemes:
Thin-layer chromatographic analysis automatic intelligent detecting instrument real-time on-line detecting method, the detecting instrument used have storage
The chromatographic sheet box of muti-piece chromatographic sheet is deposited, the development system containing liquid phase groove box supports the point sample of chromatographic sheet flat
Chromatographic sheet is moved out to sample application platform from chromatographic sheet plate box and chromatographic sheet is moved into, removes liquid phase by platform
The rotation drive device of slot box, sample spot sample device and image capture and analytical equipment can be taken automatically by above-mentioned apparatus combination
Sample, the detection method for carrying out the operating procedure of thin-layer chromatographic analysis and realizing real-time online, the detection method include:
Step 1 after chromatographic sheet box and liquid phase groove box that chromatographic sheet is housed are put into rotation drive device, is adjusted
The relative altitude of chromatographic sheet box and liquid phase groove box and sample application platform makes chromatographic sheet incline by rotating rotation drive device
Tiltedly, chromatographic sheet is slided out on sample application platform from chromatographic sheet box and is carried out by sample spot sample device under the effect of gravity
Then point sample is slid into chromatographic sheet using same principle in the liquid phase groove of liquid phase groove box；It is thin in the rotation drive device
Layer chromatography plate box and liquid phase groove box are located at sample application platform both ends, and opening corresponds to, can be by thin layer by rotating rotation drive device
Chromatosheet is moved back and forth between chromatographic sheet box and liquid phase groove box by sample application platform；
Step 2 injects the thin layer color that developing solvent carries out various functions into one or more liquid phase grooves of liquid phase groove box
Spectral expansion after expansion is disposed, extracts the developing solvent in liquid phase groove out；The bottom of the liquid phase groove connect with infusion pump and into
The injection or extraction of row developping solution, the liquid phase groove can be injected or the colour developing of spraying color developing agent progress chromatographic sheet；
Step 3 makes chromatographic sheet skid off liquid phase groove using rotation drive device, into sample application platform, chromatographic sheet
Any place of box top platform, liquid phase groove box top platform carries out image capture analysis, and described image capturing apparatus includes but unlimited
In ultraviolet, infrared scanner, industrial camera, ultraviolet irradiation, drying equipment；
Step 4 is passed through in chromatographic sheet box using the chromatographic sheet that rotation drive device will test after analysis
Recovery approach skids off the recovery bin to lower section and automatic process will prepare similarly to detect operation to next piece of new chromatographic sheet；
The thin-layer chromatography detection point of real-time online is realized by the operational order that automatic control system executes step 1 to 4
Analysis.
Preferred embodiment, the method that point sample is carried out to the chromatographic sheet above sample application platform in the step 1 are as follows: starting sample
The horizontal slide unit of product spot sample device is moved horizontally to longitudinal slide unit the top of the chromatographic sheet to point sample, and longitudinal slide unit is point
Sample head is moved down into the set distance to the chromatographic sheet of point sample, and then the liquid outlet of sampling head treats the thin layer of point sample
Chromatosheet carries out point sample, and after the completion of point sample, sample spot sample device resets to home position.
The inlet of preferred embodiment, the sampling head of the sample spot sample device is connect with infusion pump, the sampling of infusion pump into
Liquid mouth is connect with multiple chemical reaction containers or with chemical synthesis container, and sample solution is passed through point sample by automatic time sampling
Head carries out point sample on chromatographic sheet.
The thin-layer chromatographic analysis automatic intelligent detecting instrument of the utility model, including chromatographic sheet box and liquid phase groove
Box is equipped with sample application platform between chromatographic sheet box and liquid phase groove box, the opening of chromatographic sheet box and liquid phase groove box is towards point
Sample platform, the chromatographic sheet box and liquid phase groove box are connected with the lifting sliding table for adjusting itself and sample application platform relative altitude, thin
Layer chromatography plate box, liquid phase groove box and sample application platform are rotated by the synchronous driving of rotation drive device, thin in conjunction with gravity realization
Transmission of the layer chromatography plate between chromatographic sheet box, liquid phase groove box and sample application platform, the liquid phase groove box are also connected with infusion pump.
Preferred embodiment, the chromatographic sheet box include the holding mechanism that multiple chromatographic sheets can be housed, the appearance
Receiving the top of mechanism is equipped with the recovery approach of chromatographic sheet, and the top of the chromatographic sheet box, which is equipped with, places or transfer thin layer
The chromatographic sheet box top platform of chromatosheet.
Preferred embodiment, the holding mechanism of the chromatographic sheet box are that multiple first U-boards and multiple first partitions are alternate
It is formed by stacking, the opening of the multiple first U-board stores chromatographic sheet in the gap of U-shaped board towards identical.
Preferred embodiment, the liquid phase groove box is by multiple second U-shaped boards and multiple second partitions are alternate is formed by stacking, described
The opening of multiple second U-shaped boards is towards identical, and the liquid phase groove box is used to that the solvent of chromatographic sheet to be unfolded, the liquid phase groove
The liquid phase groove box top platform placed or shift chromatographic sheet is equipped at the top of box, the liquid phase trench bottom in the liquid phase groove box is set
There is the manhole appendix of circulation developing solvent, the liquid phase groove of the liquid phase groove box can be used for developing tank or colour developing slot.
Preferred embodiment, the rotation drive device include frame, and the frame is for fixing and supporting sample application platform, thin layer
The left and right ends fixation of chromatosheet box, liquid phase groove box and lifting sliding table, frame is coated with bearing rod, one end of bearing rod and motor
Output axis connection, motor by bearing rod drive the forward and reverse rotation of frame, the lifting sliding table include the first lifting sliding table and
Second lifting sliding table, the first lifting sliding table drive chromatographic sheet box mobile, and the second lifting sliding table drives liquid phase groove box mobile.
Preferred embodiment, the detecting instrument further include the sample spot sample device above the rotation drive device, institute
It states sample spot sample device and point sample operation is carried out to chromatographic sheet, the sample spot sample device includes horizontal slide unit and is located at laterally
Longitudinal slide unit on slide unit, the longitudinal slide unit are equipped with sampling head, and the liquid outlet of the sampling head is located at described in point sample
The surface of sample application platform.
Preferred embodiment, the detecting instrument further include rack, and rotation drive device and sample spot sample device are mounted on and can seal
In the rack closed, the top or front and back of the rotation drive device are equipped with the Image Acquisition that image capture is carried out to chromatographic sheet
Equipment is additionally provided with the ultraviolet lamp being irradiated to chromatographic sheet above rack；It is additionally provided in the bottom of rack to thin layer
The recovery bin that chromatosheet is collected.
Preferred embodiment is additionally provided in rack and provides the first infusion pump of developing solvent or color developing agent to liquid phase groove, and described the
The inlet of one infusion pump is connect by pipeline with the container of developing solvent or developer, and liquid outlet passes through pipeline and liquid phase groove
Manhole appendix connection；It is additionally provided with the second infusion pump of sampling in rack, the inlet of the second infusion pump passes through pipeline and sample
Container connection, liquid outlet are connect by pipeline with sampling head.
Preferred embodiment, is additionally provided with air-heater in the pedestal, and the air outlet of the air-heater is to sample application platform, thin-layer chromatography
Plate box top platform and the air-supply of liquid phase groove box top platform, sample application platform, chromatographic sheet box top platform and liquid phase groove box top
It is also equipped with heating plate on the platform of face, heating colour developing is carried out to chromatographic sheet.
Preferred embodiment, the detecting instrument further include automatic control system, and the automatic control system can network intercommunication,
User is controlled or is monitored to detecting instrument by PC computer, work station, intelligent mobile terminal.
The utility model compared with prior art, has the following advantages and benefits:
1, the utility model is automatically performed to chromatographic sheet during chromatography with a series of automation procedure
Sampling, point sample, solvent expansion, colour developing, UV scanning image capture, data analysis multinomial step, improve work efficiency.
2, the utility model provides a simple and effective rotation drive device and method for chromatographic sheet from thin layer
It is moved to sample application platform in chromatosheet box, then chromatographic sheet is moved into, liquid phase groove box is removed and realizes the thin of real-time online
Layer chromatography method can allow laboratory technician to be detached from the off-line operation of specific tlc analysis, save the working time.
3, the utility model energy automatic set point sample amount and accurate positioning point sample position, reduce artificial error, reduce inspection
The skill requirement of survey personnel.
4, the instrument of the utility model can control temperature, humidity etc. and automation procedure by sample in the environment of closing
Analysis operation experiments standardization, increases reproducibility, reduces due to artificial brought fault.
5, the combination of the chromatographic sheet box of the utility model, liquid phase groove box and rotation drive device is able to achieve high throughput
Expansion mode can detect a large amount of samples within the same expansion period, while it is for example multiple also to can be carried out diversified expansion mode
Expansion, high pressure expansion, electromagnetism expansion etc. and improve chromatography quality.
6, the utility model controls developing solvent injection liquid phase groove by infusion pump, can be carried out gradient solvent injection, furthermore
Developing solvent accurately can also be controlled and carry out horizontal thin layer expansion and 2D expansion, improve expansion resolution ratio.
7, the utility model detects the reaction process of multiple synthesis chemical reactions by sample infusion pump simultaneously.Thin-layer chromatography
The design of plate box and developing tank box can carry out real-time online tracing detection to multiple chemical reaction courses simultaneously.
8, the utility model instrument can be connect with internet and Internet of Things, and real-time Transmission image and data information pass through
PC computer or mobile terminal realize the real time monitoring to sample detection.It is able to achieve under hazardous environment such as severe toxicity, strong stimulation gas
Or the sample detection of radiation material.
Detailed description of the invention
Attached drawing described herein is used to provide to further understand the utility model embodiment, constitutes the one of the application
Part does not constitute the restriction to the utility model embodiment.In the accompanying drawings:
Fig. 1 is the structural perspective of the detecting instrument of the utility model；
Fig. 2 is the structural front view of the detecting instrument of the utility model；
Fig. 3 is the structure top view of the rotation drive device of the utility model；
Fig. 4 is cross-sectional view of the Fig. 3 along the direction A-A；
Fig. 5 is the structural perspective of the rotation drive device of the utility model；
Fig. 6 is the structural perspective of the sample spot sample device of the utility model；
Fig. 7 is the structural perspective of the chromatographic sheet box of the utility model；
Fig. 8 is the structural perspective of the liquid phase groove box of the utility model；
Fig. 9 is the operation process chart of the detecting instrument of the utility model.
Label and corresponding parts title in attached drawing:
1- rotation drive device, 2- sample spot sample device, 3- motor, 4- bearing block, 5- rack, 6- recovery bin, 7- are ultraviolet
Lamp, 8- air-heater, the first infusion pump of 9a-, the second infusion pump of 9b-, 10- chromatographic sheet, 11- frame, 12- sample application platform, 13-
Chromatographic sheet box, 14- liquid phase groove box, the first lifting sliding table of 15-, the second lifting sliding table of 16-, 17- bearing rod, 21- are laterally sliding
Platform, 22- longitudinal slide unit, 23- sampling head, 131- first partition, the first U-board of 132-, 133- chromatographic sheet box top platform,
134- recovery approach, 141- second partition, the second U-board of 142-, 145- liquid phase groove box top platform, 146- liquid phase groove.
Specific embodiment
For the purpose of this utility model, technical solution and advantage is more clearly understood, below with reference to embodiment and attached drawing,
The utility model is described in further detail, and the exemplary embodiment and its explanation of the utility model are only used for explaining this
Utility model is not intended to limit the scope of the present invention.
Embodiment
Fig. 9 shows the work flow flow chart of the detecting instrument of the utility model, thin-layer chromatographic analysis automatic intelligent
Detecting instrument real-time on-line detecting method the following steps are included:
It is put into chromatographic sheet 10 in step 1, each U-board 132 in chromatographic sheet box 13, then by thin layer color
Spectrum plate box 13 is fixed in the first lifting sliding table 15 in rotation drive device 1.
Step 2, original state are the chromatographic sheet box top platform 133 and liquid phase groove box 14 of chromatographic sheet box 13
Liquid phase groove box top platform 145 is flushed with the top of sample application platform 12.Start automated procedures operation, 15 band of the first lifting sliding table
Dynamic chromatographic sheet box 13 moves up, until first containing chromatographic sheet 10 U-board in chromatographic sheet box 13
132 open end is completely exposed, so that the chromatographic sheet 10 in U-shaped board 132 can skid off, the second lifting sliding table 16 drives liquid phase
Slot box 14 moves up, until the second partition 141 in liquid phase groove 14 exposes.
Step 3 drives rotation drive device 1 to rotate towards 14 direction of liquid phase groove box by bearing rod 17, chromatographic sheet 10
It skids off from chromatographic sheet box 13 to above sample application platform 12, chromatographic sheet 10 is by the second partition 141 of liquid phase groove box 14
It is blocked on sample application platform 12,1 opposite direction of rotation drive device is then rotated to horizontal position.
The sliding block of step 4, mobile example spot sample device horizontal slide unit 21, by the sampling head 23 in longitudinal slide unit 22 from first
Beginning position is moved to the setting position of the top of rotation drive device 1, and the sampling head 23 declined in longitudinal slide unit 22 arrives thin layer color
Compose the setting position of plate 10.Start the second infusion pump 9b sampling, sample is injected in thin layer color by contact or cordless
It composes on plate 10.Sampling head 23 is moved back to initial position after point sample is complete, cleans sampling head 23, repeats aforesaid operations for the 2nd sample
Point on chromatographic sheet 10, until detection in need sample spot it is complete until.
The sample solvent that step 5, volatilize naturally point sample solvent or unlatching air-heater 8 volatilize on chromatographic sheet 10；Second
Lifting sliding table 16, which moves up liquid phase groove box 14, allows the notch of liquid phase groove box 14 to expose.Then it is rotated towards 14 direction of liquid phase groove box
Rotary driving device 1 allows chromatographic sheet 10 to slide into as in the liquid phase groove 146 of developing tank, rotation drive device 1 is rotated to perpendicular
The state of straight and liquid phase groove box 14 notch upward, the second lifting sliding table 16 moves down liquid phase groove box 14, so that sample application platform
12 side wall covers the notch of liquid phase groove box 14.
The first infusion pump 9a that step 6, starting are connect with expansion liquid container, inputs liquid phase groove for quantitative developing solvent
The solvent expansion of sample composition is carried out in 146.After being unfolded, developing solvent is taken out from liquid phase groove 146 with the first infusion pump 9a
Out.First lifting sliding table 15 moves down chromatographic sheet box 13, the chromatographic sheet box top platform of chromatographic sheet box 13
133 flush with sample application platform 12, and the second lifting sliding table 16 moves up liquid phase groove box 14, the slot of the developing tank of liquid phase groove box 14
Mouth exposes, and reversely rotates rotation drive device 1, the chromatographic sheet 10 in developing tank is allowed to skid off thin layer color by sample application platform
It composes on plate box top platform 133, rotation drive device 1 is then rotated to horizontality, to the chromatographic sheet 10 after expansion
Carry out solvent volatilization.Second lifting sliding table 16 moves down liquid phase groove box 14, the liquid phase groove box top platform of liquid phase groove box 14
145 flush with sample application platform 12, then rotate rotation drive device 1 towards 14 direction of liquid phase groove box, and chromatographic sheet 10 is allowed to slide into
On liquid phase groove box top platform 145, rotation drive device 1 is then rotated to horizontality, be then turned on ultraviolet lamp 7 irradiation and
Start image capture device and carries out UV scanning and image capture.It, will expansion if to carry out color development treatment to colourless Sample
Chromatographic sheet 10 afterwards slides into the liquid phase groove 146 for being used as colour developing, injection or spraying color developing agent according to same process, has handled
After drain color developing agent, chromatographic sheet 10 is slided into chromatographic sheet box top platform 133 or liquid phase groove box top with same process
Heating colour developing is carried out on face platform 145, then or simultaneously carries out the image capture of 10 sample of chromatographic sheet.
Step 7, the first lifting sliding table move up chromatographic sheet box 13, so that the recovery approach of chromatographic sheet box 13
134 is parallel with point sample operation post, rotates rotation drive device 1 towards 13 direction of chromatographic sheet box, by chromatographic sheet 10 from liquid
It is slid into recovery bin 6 on phase slot box top platform 145 by recovery approach 134.
Step 8 repeats step 2 to 7 according to automation procedure, skids off the 2nd piece of chromatographic sheet 10 with identical or different
Liquid phase groove 146 carries out the point sample of identical or different sample, and solvent expansion, solvent volatilization, colour developing and the automation of Image Acquisition are grasped
Make and realizes real-time online and detect.
Preferred embodiment scheme, all or part of apparatus are suitable for other sides for utilizing same thin plate sample analysis
Method includes but is not limited to electrophoretic analysis, and cell dyeing method etc. can use this all or part of apparatus structure thus by these
Determination method is implemented as the analysis method of real-time online.
As depicted in figs. 1 and 2, the utility model provides thin-layer chromatographic analysis automatic intelligent detecting instrument, including right
Chromatographic sheet 10 carries out mobile rotation drive device 1, and referring to figure 3., shown in Fig. 4, Fig. 5, rotation drive device 1 includes:
Frame 11, frame 11 are preferably but not limited to be preferably but not limited to for " Kan " shape, sample application platform 12 as " Jiong " shape, frame 11 or so two
The medium position at end is fixed to be coated with bearing rod 17, and the left and right ends of bearing rod 17 have worn bearing block 4 respectively, and bearing block 4 is solid
It is scheduled in rack 5.One end of bearing rod 17 is connect by shaft coupling with the rotor of output shaft axle of motor 3, and motor 3 is fixed on rack 5
On, motor 3 drives 1 forward and reverse rotation of rotation drive device by bearing rod 17.Sample application platform 12, point are fixedly mounted in frame 11
Sample platform 12 is 11 equipartition of frame at the first operating area, that is, 13 storage area of chromatographic sheet box and the second operating area
That is 14 storage area of liquid phase groove box, the first operating area and the second operating area are located at the left and right sides of frame 11.Frame 11
It is equipped with the first lifting sliding table 15 and chromatographic sheet box 13 in first operating area, the second lifting is equipped in the second operating area and is slided
Platform 16 and liquid phase groove box 14, for sample application platform 12, the first lifting sliding table 15 in the first operating area drives thin layer
Chromatosheet box 13 moves up and down, and chromatographic sheet box 13 is removably connected to the sliding shoe of the first lifting sliding table 15
On, chromatographic sheet box 13 is exhausted with chromatographic sheet 10, can be replaced in rear before detection or detection, to guarantee to sample
Product carry out prolonged lasting detection.For sample application platform 12, the second lifting sliding table 16 in the second operating area
Sliding shoe drives liquid phase groove box 14 to move up and down, and different chromatographic sheets 10 is sent into sample in liquid phase groove box 14 with realizing
The step of carrying out solvent expansion, colour developing.The open end of chromatographic sheet box 13 and the open end of liquid phase groove box 14 are both facing to point sample
Platform 12, in order to which chromatographic sheet 10 in chromatographic sheet box 13 from skidding off into any liquid phase groove box 14, sample application platform
The side wall at 12 both ends is adjacent with the open end of the open end of chromatographic sheet box 13 and liquid phase groove box 14 respectively, thin for closing
The open end of layer chromatography plate box 13 and the open end of liquid phase groove box 14, to open 13 open end of chromatographic sheet box and liquid phase groove
14 open end of box only need to be moved upwards up to setting position by the first lifting sliding table 15 and the second lifting sliding table 16.
It please refers to shown in Fig. 7, chromatographic sheet box 13 is that multiple first U-boards 132 and multiple first partitions 131 are alternate folded
Add, particular number can be increased or decreased according to specific works amount, and the opening direction of multiple U-boards 132 is identical, thin layer
The top of chromatosheet box 13 is equipped with the chromatographic sheet box top platform 133 placed or shift chromatographic sheet 10, thin-layer chromatography
The left and right sides of plate box top platform 133 and the opposite side of open end are equipped with coaming plate, prevent chromatographic sheet 10 in thin-layer chromatography
Deviate from plate box top platform 133.In the top of chromatographic sheet box top platform 133, installation air-heater 8 is to chromatographic sheet
10 are dried, and the most upper interlayer of chromatographic sheet box 13 is additionally provided with the recovery approach 134 of chromatographic sheet 10, recovery approach
134 left and right ends are equipped with the coaming plate thicker than 10 thickness of chromatographic sheet, and rear and front end communicates, convenient for the thin layer color after using
Spectrum plate 10 skids off rotation drive device 1 by recovery approach 134, falls on the inner centralized collection of recovery bin 6 of lower section.
Please refer to shown in Fig. 8, liquid phase groove box 14 be by multiple with the first U-shaped board 132 and alternate be superimposed of first partition 131 and
At, or second U-shaped board 142 thicker than the first U-shaped board 132 and first partition 131 and second partition 141 is alternate is formed by stacking,
The opening direction of liquid phase groove box 14 is identical, sample application platform 12 of the notch towards centre.It is equipped at the top of liquid phase groove box 14 and places or turn
The liquid phase groove box top platform 145 of chromatographic sheet 10 is moved, chromatographic sheet 10 can be on liquid phase groove box top platform 145
It taken pictures, recorded a video or is scanned by the image capture device that top is installed.The bottom of liquid phase groove box 14 is equipped with liquid manhole appendix, can
Developing solvent or color developing agent are injected liquid phase groove box 14 or developing solvent or developer from liquid phase by the first infusion pump 9a
It is extracted out in slot box 14.Liquid phase groove 146 as colour developing slot can also be equipped with spray channel on the side wall of liquid phase groove 146.Liquid phase groove
It can also be equipped with the positive and negative electrode of connection DC power supply in 146, DC electric field can be applied and carry out the thin layer solvent exhibition under electric field
It opens, and accelerates sample composition of the separation on chromatographic sheet 10.
Preferred embodiment scheme, as shown in Fig. 1 Fig. 2 and Fig. 6, thin-layer chromatographic analysis automatic intelligent detecting instrument is also wrapped
The sample spot sample device 2 for being located at 1 top of rotation drive device is included, sample spot sample device 2 carries out cooperateing with work with rotation drive device 1
Industry, the effect of sample spot sample device 2 are that, to the progress point sample operation of chromatographic sheet 10, sample spot sample device 2 includes horizontal slide unit
21 and the longitudinal slide unit 22 that is fixed in horizontal slide unit 21, sampling head 23 is fixed in longitudinal slide unit 22, and sampling head 23 goes out liquid
Mouth is in point sample positioned at the surface of sample application platform 12.When chromatographic sheet 10 needs to carry out point sample, horizontal slide unit 21 first
Longitudinal slide unit 22 is moved to the surface of sample application platform 12, the upper surface of sample application platform 12 placed the chromatographic sheet to point sample
10, next, longitudinal slide unit 22 drives sampling head 23 to move down, until liquid outlet and the chromatographic sheet 10 of sampling head 23 connect
Stop when close, can guarantee sample on chromatographic sheet 10 when sampling head 23 carries out point sample to chromatographic sheet 10 in this way
It repeats to be accurately positioned.Longitudinal slide unit 22 on sample spot sample device 2 in order not to interfere a series of rotations of rotation drive device 1,
Just pass through horizontal slide unit 21 in point sample to be moved to point sample above rotation drive device 1, according to the position pair set
Chromatographic sheet 10 carries out point sample.
Preferred embodiment scheme, as depicted in figs. 1 and 2, rotation drive device 1 and sample spot sample device 2 are mounted on rack 5
Interior, the top of rotation drive device 1, which is equipped with, is scanned/shooting/image capture device recorded a video, pedestal to chromatographic sheet 10
5 top is additionally provided with to the ultraviolet lamp 7 being irradiated above rotation drive device 1, and the bottom of rack 5 is additionally provided with to thin-layer chromatography
The recovery bin 6 that plate 10 is collected is additionally provided in rack 5 and provides the first infusion of developing solvent or color developing agent to liquid phase groove box 14
9a is pumped, the first infusion pump 9a is preferably but not limited to as peristaltic pump, reciprocating pump, vacuum pump etc..The feed liquor of first infusion pump 9a
Mouth is connect by pipeline with developing solvent or reagent container, and liquid outlet is connected by pipeline and the liquid manhole appendix of liquid phase groove 146
It connects, is additionally provided with air-heater 8 in pedestal 5, the air outlet of air-heater 8 passes through to ventilation shaft to thin above rotation drive device 1
Layer chromatography plate box top platform 133 or liquid phase groove box top platform 145 are blown, and the drying to chromatographic sheet 10 is accelerated.
Preferred embodiment scheme, the inlet of the second infusion pump 9b of thin-layer chromatographic analysis automatic intelligent detecting instrument with
Sample container is connected by pipeline, can carry out automatic sampling printing operation by automated procedures.In synthesis chemical reaction inspection
In survey, multiple reaction vessels can be connected simultaneously, the content for detecting reactant or product change with time and principal reaction into
Journey.
Preferred embodiment scheme, thin-layer chromatographic analysis automatic intelligent detecting instrument have automatic control system, the control
System processed includes but is not limited to PC computer, PLC control panel, motion control card, motor, motor driving plate and image analysis and storage
And sample software for calculation；The automatic control system is connect with internet and Internet of Things, user by PC computer, work station,
Intelligent mobile terminal is controlled or is monitored to detecting instrument.
Above-described specific embodiment, to the purpose of this utility model, technical scheme and beneficial effects carried out into
One step is described in detail, it should be understood that being not used to limit the foregoing is merely specific embodiment of the present utility model
Determine the protection scope of the utility model, within the spirit and principle of the utility model, any modification for being made equally is replaced
It changes, improve, should be included within the scope of protection of this utility model.
Claims (10)
1. thin-layer chromatographic analysis automatic intelligent detecting instrument, which is characterized in that thin including chromatographic sheet box and liquid phase groove box
Sample application platform is equipped between layer chromatography plate box and liquid phase groove box, the opening of chromatographic sheet box and liquid phase groove box is flat towards point sample
Platform, the chromatographic sheet box and liquid phase groove box are connected with the lifting sliding table for adjusting itself and sample application platform relative altitude, thin layer color
Compose plate box, liquid phase groove box and sample application platform are rotated by the synchronous driving of rotation drive device, in conjunction with gravity realization thin layer color
Transmission of the plate between chromatographic sheet box, liquid phase groove box and sample application platform is composed, the liquid phase groove box is also connected with infusion pump.
2. thin-layer chromatographic analysis automatic intelligent detecting instrument according to claim 1, which is characterized in that the thin layer color
Spectrum plate box includes the holding mechanism that multiple chromatographic sheets can be housed, and the top of the holding mechanism is equipped with chromatographic sheet
Recovery approach, the top of the chromatographic sheet box is equipped with placement or the chromatographic sheet box top of transfer chromatographic sheet is flat
Platform.
3. thin-layer chromatographic analysis automatic intelligent detecting instrument according to claim 2, which is characterized in that the thin layer color
The holding mechanism of spectrum plate box is multiple first U-boards and multiple first partitions are alternate is formed by stacking, the multiple first U-board
Opening stores chromatographic sheet in the gap of U-shaped board towards identical.
4. thin-layer chromatographic analysis automatic intelligent detecting instrument according to claim 1, which is characterized in that the liquid phase groove
Box is by multiple second U-shaped boards and multiple second partitions are alternate is formed by stacking, and the opening of the multiple second U-shaped board is towards phase
Together, the liquid phase groove box is used to that the solvent of chromatographic sheet to be unfolded, and placement or transfer thin layer are equipped at the top of the liquid phase groove box
The liquid phase groove box top platform of chromatosheet, the liquid phase trench bottom in the liquid phase groove box are equipped with the manhole appendix of circulation developing solvent,
The liquid phase groove of the liquid phase groove box can be used for developing tank or colour developing slot.
5. thin-layer chromatographic analysis automatic intelligent detecting instrument according to claim 1, which is characterized in that the rotation is driven
Dynamic device includes frame, and the frame is slided for fixing and supporting sample application platform, chromatographic sheet box, liquid phase groove box and lifting
The left and right ends fixation of platform, frame is coated with bearing rod, one end of bearing rod and the output axis connection of motor, and motor passes through bearing
Bar drives the forward and reverse rotation of frame, and the lifting sliding table includes the first lifting sliding table and the second lifting sliding table, the first lifting sliding table
Drive chromatographic sheet box mobile, the second lifting sliding table drives liquid phase groove box mobile.
6. thin-layer chromatographic analysis automatic intelligent detecting instrument according to claim 1, which is characterized in that the detector
Device further includes the sample spot sample device above the rotation drive device, the sample spot sample device to chromatographic sheet into
Row point sample operation, the sample spot sample device include horizontal slide unit and the longitudinal slide unit in horizontal slide unit, and the longitudinal direction is sliding
Platform is equipped with sampling head, and the liquid outlet of the sampling head is in point sample positioned at the surface of the sample application platform.
7. thin-layer chromatographic analysis automatic intelligent detecting instrument according to claim 1-6, which is characterized in that institute
Stating detecting instrument further includes rack, and rotation drive device and sample spot sample device are mounted in closed rack, the rotation
The top or front and back of driving device be equipped with to chromatographic sheet carry out image capture image capture device, the top of rack also
Equipped with the ultraviolet lamp being irradiated to chromatographic sheet；The recycling being collected to chromatographic sheet is additionally provided in the bottom of rack
Storehouse.
8. thin-layer chromatographic analysis automatic intelligent detecting instrument according to claim 7, which is characterized in that also set in rack
Have and provide the first infusion pump of developing solvent or color developing agent to liquid phase groove, the inlet of first infusion pump passes through pipeline and exhibition
The container connection of solvent or developer is opened, liquid outlet is connected by pipeline and the manhole appendix of liquid phase groove；Sampling is additionally provided in rack
The inlet of second infusion pump, the second infusion pump is connected by the container of pipeline and sample, and liquid outlet passes through pipeline and point
The connection of sample head.
9. thin-layer chromatographic analysis automatic intelligent detecting instrument according to claim 7, which is characterized in that in the rack
It is additionally provided with air-heater, the air outlet of the air-heater is to sample application platform, chromatographic sheet box top platform and liquid phase groove box top
Platform is blown, and is also equipped with heating plate to thin on sample application platform, chromatographic sheet box top platform and liquid phase groove box top platform
Layer chromatography plate carries out heating colour developing.
10. thin-layer chromatographic analysis automatic intelligent detecting instrument according to claim 7, which is characterized in that the detection
Instrument further includes automatic control system, and the automatic control system can network intercommunication, and user passes through PC computer, work station, intelligence
Energy mobile terminal is controlled or is monitored to detecting instrument.
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| CN201820516945.2U CN208283346U (en) | 2018-04-12 | 2018-04-12 | Thin-layer chromatographic analysis automatic intelligent detecting instrument |
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| CN201820516945.2U CN208283346U (en) | 2018-04-12 | 2018-04-12 | Thin-layer chromatographic analysis automatic intelligent detecting instrument |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108459133A (en) * | 2018-04-12 | 2018-08-28 | 成都爱恩通医药科技有限公司 | Thin-layer chromatographic analysis automatic intelligent detecting instrument and its real-time on-line detecting method |
| CN110531023A (en) * | 2019-10-08 | 2019-12-03 | 安徽理工大学 | A kind of thin-layer chromatography analyzer |
| CN110632239A (en) * | 2019-10-21 | 2019-12-31 | 清华大学 | A kind of sampling device and method for element speciation analysis based on thin-layer chromatography |
| CN111189955A (en) * | 2020-01-17 | 2020-05-22 | 浙江大学 | A method for determining the type of natural products based on the color change information in the TLC dyeing process |
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2018
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108459133A (en) * | 2018-04-12 | 2018-08-28 | 成都爱恩通医药科技有限公司 | Thin-layer chromatographic analysis automatic intelligent detecting instrument and its real-time on-line detecting method |
| WO2019196902A1 (en) * | 2018-04-12 | 2019-10-17 | 成都爱恩通医药科技有限公司 | Automated intelligent detection instrument for thin-layer chromatography and real-time online detection method thereof |
| CN108459133B (en) * | 2018-04-12 | 2024-04-23 | 成都爱恩通医药科技有限公司 | Automatic intelligent detection instrument for thin-layer chromatography analysis and real-time online detection method thereof |
| CN110531023A (en) * | 2019-10-08 | 2019-12-03 | 安徽理工大学 | A kind of thin-layer chromatography analyzer |
| CN110632239A (en) * | 2019-10-21 | 2019-12-31 | 清华大学 | A kind of sampling device and method for element speciation analysis based on thin-layer chromatography |
| CN111189955A (en) * | 2020-01-17 | 2020-05-22 | 浙江大学 | A method for determining the type of natural products based on the color change information in the TLC dyeing process |
| CN111189955B (en) * | 2020-01-17 | 2021-05-25 | 浙江大学 | Method for judging natural product category based on color change information in thin-layer chromatography dyeing process |
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