CN117503658B

CN117503658B - Multi-effect composition containing recombinant collagen and preparation method and application thereof

Info

Publication number: CN117503658B
Application number: CN202311662932.8A
Authority: CN
Inventors: 郭朝万; 王宁; 孙云起; 裴运林; 江川霞; 蒲艳
Original assignee: Guangdong Marubi Biological Technology Co Ltd
Current assignee: Guangdong Marubi Biological Technology Co Ltd
Priority date: 2023-12-05
Filing date: 2023-12-05
Publication date: 2024-06-18
Anticipated expiration: 2043-12-05
Also published as: CN117503658A; WO2025118836A1

Abstract

本发明提供一种含有重组胶原蛋白的多效组合物及其制备方法和应用，所述多效组合物包括重组胶原蛋白、古朴阿苏果纳米胶囊、超分子木棉花和超分子肌肽。本发明提供的多效组合物中采用古朴阿苏果纳米胶囊，有效提高了功能活性成分的生物利用率，超分子肌肽能够保护胶原蛋白和弹性蛋白不受氧化应激损害，从而保持良好的皮肤结构，具备抗糖化作用，将具有抗氧化作用的超分子木棉花和超分子肌肽、胶原蛋白、古朴阿苏果提取物搭配使用，协同作用多效解决肌肤问题，在抗氧化、促进皮肤再生、抗糖化的同时进一步提高补水保湿的效果。The present invention provides a multi-effect composition containing recombinant collagen, a preparation method and an application thereof, wherein the multi-effect composition comprises recombinant collagen, cupuacu fruit nanocapsules, supramolecular kapok flowers and supramolecular carnosine. The cupuacu fruit nanocapsules are used in the multi-effect composition provided by the present invention, which effectively improves the bioavailability of functional active ingredients, and supramolecular carnosine can protect collagen and elastin from oxidative stress damage, thereby maintaining a good skin structure and having an anti-glycation effect. The supramolecular kapok flowers with an antioxidant effect are used in combination with supramolecular carnosine, collagen and cupuacu fruit extract, and the synergistic effect solves skin problems in a multi-effect manner, and further improves the effect of moisturizing and moisturizing while resisting oxidation, promoting skin regeneration and resisting glycation.

Description

Multi-effect composition containing recombinant collagen and preparation method and application thereof

Technical Field

The invention belongs to the technical field of skin care products, and particularly relates to a multi-effect composition containing recombinant collagen, a preparation method and application thereof.

Background

With the aging, the body functions are reduced, the skin epidermis tissue is dried and thinned, collagen and elastin are gradually lost, the fine mesh structure formed by interweaving collagen fibers and elastic fibers is damaged, wrinkles with different thicknesses can occur, and besides the natural aging of the body skin, many factors in the environment can accelerate the skin aging process, such as excessive sugar intake, ultraviolet radiation, bad life style and the like. Based on the factors influencing skin aging, active substances with functions of resisting saccharification, resisting oxidization, resisting photoaging, promoting skin repair and the like are of great significance for resisting skin aging.

The collagen has the stretching capability, can keep skin elasticity and promote cell growth, can play roles in moisturizing, repairing, resisting wrinkles and the like when being applied to skin care products, and has good biodegradability and biological cosmetology, so that the collagen is also increasingly applied to skin care products.

The ancient magnolia-assuo fruit is a fruit growing in the tropical rain forest of North Amazon of Brazil. Its fruit contains rich vitamins, cellulose and amino acids, and is a natural antioxidant. The natural grease similar to butter can be extracted from seeds, has rich vitamins (especially vitamin A and vitamin C), mineral substances, amino acids, fatty acids and flavonoids, has great benefits to human bodies, can lead the skin to be fine, moist and smooth after being smeared for a long time, has excellent moisturizing and skin repairing promoting effects, has good absorption to ultraviolet rays, has no stimulation to the skin, has good safety, can effectively absorb the ultraviolet rays in sunlight, has a certain nursing effect on preventing sunburn and redness of the skin after sun, and is an unprecedented food material and natural skin care product for local people.

The kapok extract is rich in triterpene components and flavonoid compounds, can remove free radicals, promote the synthesis of aquaporin 3 and hyaluronic acid, rapidly supplement moisture for skin cells, improve skin elasticity, and repair fine wrinkles. Meanwhile, the kapok is used as a traditional Chinese medicine component, has sweet and cool taste, has the effects of clearing heat and promoting diuresis, removing toxicity and stopping bleeding, and has certain anti-inflammatory and repairing effects after being externally applied.

CN102836093a discloses a face cream containing type I collagen, the mass percentage composition of the face cream is: 3-8% of glycerin, 1-3% of trehalose, 0.1-0.3% of methyl hydroxybenzoate, 0.05-0.2% of propyl hydroxybenzoate, 0.1-0.5% of allantoin, 0.5-2% of VC ethyl ether, 0.5-2% of vitamin B, 0.5-1% of dipotassium glycyrrhizinate, 1-2% of M68, 1-10% of A165, 1-2% of 16-18 alcohol, 200-2% of DC, 2-10% of GTCC, 1.5-10% of shea butter, 3-6% of Celastracea butter, 1-5% of 2EHP, 0.1-1% of vitamin A acetate, 0.4-1% of vitamin E acetate, 0.01-0.1% of piperine, 0.5-2% of type I collagen, 0.01-0.05% of essence, 0.1-0.5% of S-305 and 1-5% of LS 8740; has the effects of increasing skin tightness, reducing deep wrinkles, smoothing fine lines, compacting and improving skin elasticity from deep layers, and has obvious effects of moistening, moisturizing and resisting aging.

CN106109277a provides a moisturizing composition comprising an amino acid humectant, a cupola oil and an adjuvant. The content of the mangnolia officinalis and the perilla fruit oil is limited, and the mangnolia officinalis and the perilla fruit oil can fully play the role of moisturizing activity in the proportion, and have synergistic effect with an amino acid humectant, so that the moisturizing composition has good moisturizing effect.

CN113081874a discloses that kapok extract and decarboxylated carnosine are added separately to different cosmetic formulations to make personalized cosmetics for different skin conditions.

However, in the prior art, the ancient hackberry extract and kapok extract still have the problems of slow onset of action, insignificant effect and the like caused by low skin bioavailability.

Therefore, the development of a composition can match collagen with the extract of the ancient hackberry and the extract of the kapok to achieve various effects of promoting skin repair and regeneration, resisting the aging of the skin, resisting the oxidation, resisting the saccharification and the like, and is the research focus in the field.

Disclosure of Invention

Aiming at the defects of the prior art, the invention aims to provide a multi-effect composition containing recombinant collagen, a preparation method and application thereof, and the composition has various effects of promoting skin repair and regeneration, resisting aging, resisting oxidation, resisting saccharification and the like.

In order to achieve the aim of the invention, the invention adopts the following technical scheme:

In a first aspect, the invention provides a pleiotropic composition comprising recombinant collagen, wherein the pleiotropic composition comprises recombinant collagen, a cupro nanocapsule, supramolecular kapok and supramolecular carnosine.

The multi-effect composition provided by the invention has the functions of promoting skin regeneration, resisting oxidation, resisting light aging and the like, the ancient Chinese magnolia and perilla fruit nanocapsules are adopted, so that the bioavailability of functional active ingredients is effectively improved; the supermolecular carnosine can protect collagen and elastin from oxidative stress, so that a good skin structure is maintained, and the supermolecular kapok with an antioxidant effect and the supermolecular carnosine with an anti-saccharification effect are added, so that the supermolecular carnosine can play a synergistic effect with collagen and a mangnolia officinalis extract, and the effects of moisturizing and moisturizing are further improved while the oxidation is resisted.

Preferably, the multi-effect composition comprises 0.1-0.5 part by weight of recombinant collagen, for example, 0.2 part, 0.3 part, 0.4 part and the like, 1-10 parts by weight of Guo Po-A Su fruit nanocapsules, for example, 2 parts, 4 parts, 6 parts, 8 parts and the like, 1-10 parts by weight of supermolecule kapok, for example, 2 parts, 4 parts, 6 parts, 8 parts and the like, and 0.1-1 part by weight of supermolecule carnosine, for example, 0.2 parts, 0.4 parts, 0.6 parts, 0.8 parts and the like.

Preferably, the Guo's apo-su-fruit nanocapsules are prepared by a method comprising:

(1) Dissolving the ancient hackberry oil and the pramipexole in acetone to obtain a mixed solution;

(2) Dripping the mixed solution into distilled water under stirring, continuously stirring, and removing acetone by natural evaporation to obtain nanoparticles;

(3) And adding chitosan into the continuously stirred nano particles to obtain the Guo-aposu-fruit nano capsule.

Preferably, the pramipexole has a hydrophilic-lipophilic balance of 8-29, which may be, for example, 10, 12, 14, 16, 18, 20, 22, 24, 26, 28, etc.

Preferably, the molecular weight of the chitosan is 3-20kDa, for example, it may be 4kDa, 6kDa, 8kDa, 10kDa, 12kDa, 14kDa, 16kDa, 18kDa, etc.

Preferably, the mass ratio of the ancient aposu fruit oil to the pramipexole is 1 (3-5), and for example, the mass ratio can be 1:3.5, 1:4, 1:4.5 and the like.

Preferably, the mass to volume ratio of the Guo's assuo oil to the acetone is (4-6) mg/1 mL, for example, 4.5 mg/1 mL, 5 mg/1 mL, 5.5 mg/1 mL, etc.

Preferably, the distilled water is used in an amount of 4 times that of acetone.

Preferably, the particle size of the Guo's apo-su fruit nanocapsules is 30-60nm, for example, 35nm, 40nm, 45nm, 50nm, 55nm and the like.

Preferably, the supermolecular kapok is prepared by a method comprising: extracting kapok pollen powder with DES-TG aqueous solution.

Preferably, the feed liquid ratio of the kapok powder to the DES-TG aqueous solution is 1 (30-70), for example, 1:40, 1:50, 1:60 and the like.

Preferably, the DES-TG aqueous solution comprises betaine, glycerol and propylene glycol.

In a second aspect, the present invention provides a method for preparing a recombinant collagen-containing multi-effect composition according to the first aspect, the method comprising: mixing recombinant collagen, guo Po-A-Su-Guo nanocapsule, supramolecular kapok and supramolecular carnosine.

In a third aspect, the invention provides an antioxidant moisturizing emulsion, which comprises the following raw materials: the multi-effect composition comprising recombinant collagen, emollient, humectant, emulsifier, and thickener of the first aspect.

Preferably, the mass percentage of the multi-effect composition in the antioxidant moisturizing emulsion is 1-10%, for example, 2%, 4%, 6%, 8%, 10% and the like.

The numerical ranges recited herein include not only the recited point values, but also any point values between the recited numerical ranges that are not recited, and are limited to, and for the sake of brevity, the invention is not intended to be exhaustive of the specific point values that the recited range includes.

Compared with the prior art, the invention has the following beneficial effects:

The multi-effect composition provided by the invention adopts the ancient-bark-amomum-perilla fruit nanocapsules, so that the bioavailability of functional active ingredients is effectively improved; the supermolecular carnosine can protect collagen and elastin from oxidative stress, so that a good skin structure is maintained, and the supermolecular carnosine has an anti-glycation effect; the supramolecular kapok with the antioxidation function and supramolecular carnosine, collagen and the extract of the ancient magnolia and perilla fruit are matched for use, and the synergistic effect is multiple-effect to solve the skin problem, so that the effects of moisturizing and moisturizing are further improved while the antioxidation, the skin regeneration promotion and the saccharification resistance are realized.

Detailed Description

The technical scheme of the invention is further described by the following specific embodiments. It will be apparent to those skilled in the art that the examples are merely to aid in understanding the invention and are not to be construed as a specific limitation thereof.

The terms "comprising," "including," "having," "containing," or any other variation thereof, are intended to cover a non-exclusive inclusion. For example, a composition, step, method, article, or apparatus that comprises a list of elements is not necessarily limited to only those elements but may include other elements not expressly listed or inherent to such composition, step, method, article, or apparatus.

"Optional" or "any" means that the subsequently described event or event may or may not occur, and that the description includes both cases where the event occurs and cases where the event does not.

The indefinite articles "a" and "an" preceding an element or component of the invention are not limited to the requirement (i.e. the number of occurrences) of the element or component. Thus, the use of "a" or "an" should be interpreted as including one or at least one, and the singular reference of an element or component includes the plural reference unless the amount clearly dictates otherwise.

The description of the terms "one embodiment," "some embodiments," "exemplarily," "specific examples," or "some examples," etc., herein described means that a specific feature, structure, material, or characteristic described in connection with the embodiment or example is included in at least one embodiment or example of the present invention. In this document, the schematic representations of the above terms are not necessarily for the same embodiment or example.

In the present invention, percentages and percentages are by mass unless explicitly stated otherwise. Unless otherwise specified, all experimental procedures used are conventional and all materials, reagents, etc. used are commercially available.

Recombinant collagen: starting point biological company, fully human ^TM collagen, molecular weight 20-40KD;

ancient magnolia and perilla oil: craien, refined Butter；

Kapok: the pharmaceutical company of the bayer process,Kapok;

Carnosine: dragosine, germany, inc.

Preparation example 1

The preparation example provides a Guo Po and Suguo nano capsule, which is prepared by the following steps:

(1) 5mg of Guo Po-A Perilla fruit oil and 20mg of Pranik P85 are dissolved in 1mL of acetone to obtain a mixed solution;

(2) Dripping the mixed solution into 4mL of distilled water stirred at 400rpm, continuously stirring for 7h, and removing acetone by natural evaporation to obtain nano particles;

(3) To the continuously stirred nanoparticle was added 20mg chitosan (molecular weight 15 kDa) to obtain Guo Po-A-Suguo nanocapsules (particle size 40 nm).

Comparative preparation example 1

The comparative preparation example provides a Guo-aposu nanocapsule which is different from the preparation example only in that acetone is replaced by the same amount of ethanol, and other raw materials, amounts and preparation methods are referred to in preparation example 1.

Preparation example 2

The preparation example provides supermolecule kapok, and the preparation method comprises the following steps:

(1) Drying flos kapok, pulverizing, and sieving with 30 mesh sieve to obtain powder. Adding water into betaine, glycerol and propylene glycol according to a mass ratio of 20:60:3 to prepare a 40% supermolecule extract;

(2) 10g of kapok powder is taken, 1g of kapok powder and 40mL of supermolecule extracting solution are added, and high-voltage pulse extraction is carried out for 80 times with the voltage of 600V. And carrying out suction filtration after the extraction is finished to obtain the supermolecule kapok.

Preparation example 3

This preparation provides a supramolecular carnosine prepared with reference to example 2 of CN114306113 a.

Example 1

The present embodiment provides a multi-effect composition comprising:

0.3 part of recombinant collagen, 8 parts of a Guo-aposu fruit nano capsule of preparation example 1, 8 parts of supermolecule kapok of preparation example 2 and 0.3 part of supermolecule carnosine of preparation example 3; the raw materials are uniformly mixed according to the formula amount to obtain the multi-effect composition.

Example 2

The present embodiment provides a multi-effect composition comprising:

0.5 part of recombinant collagen, 6 parts of Guo-ao-a-su-fruit nanocapsules of preparation example 1, 10 parts of supramolecular kapok of preparation example 2 and 0.1 part of supramolecular carnosine of preparation example 3; the raw materials are uniformly mixed according to the formula amount to obtain the multi-effect composition.

Example 3

The present embodiment provides a multi-effect composition comprising:

0.1 part of recombinant collagen, 10 parts of Guo-ao-a-su-fruit nanocapsules of preparation example 1, 6 parts of supermolecule kapok of preparation example 2 and 0.5 part of supermolecule carnosine of preparation example 3; the raw materials are uniformly mixed according to the formula amount to obtain the multi-effect composition.

Example 4

The present embodiment provides a multi-effect composition comprising:

0.3 part of recombinant collagen, 3 parts of a Guo-aposu fruit nano capsule of preparation example 1,2 parts of supermolecule kapok of preparation example 2 and 0.9 part of supermolecule carnosine of preparation example 3; the raw materials are uniformly mixed according to the formula amount to obtain the multi-effect composition.

Example 5

The present embodiment provides a multi-effect composition comprising:

0.3 part of recombinant collagen, 12 parts of a Guo-aposu fruit nano capsule of preparation example 1, 12 parts of supermolecule kapok of preparation example 2 and 2 parts of supermolecule carnosine of preparation example 3; the raw materials are uniformly mixed according to the formula amount to obtain the multi-effect composition.

Example 6

This example provides a multi-effect composition that differs from example 1 only in that the Guo's apo-su-fruit nanocapsules of preparation example 1 are adjusted to be equal in amount to the Guo's apo-su-fruit nanocapsules of comparative preparation example 1, and other raw materials, amounts and preparation methods are referred to in example 1.

Comparative example 1

This comparative example provides a multi-effect composition which differs from example 1 only in that 8 parts of the cupola-attuo nanocapsules of preparation example 1 are replaced with 8 parts of cupola-attuo oil, and other raw materials, amounts and preparation methods are referred to in example 1.

Comparative example 2

This comparative example provides a multi-effect composition which differs from example 1 only in that 8 parts of the supermolecule kapok of preparation example 2 was replaced with 8 parts of kapok extract, and other raw materials, amounts and preparation methods are referred to in example 1.

Comparative example 3

This comparative example provides a multi-effect composition which differs from example 1 only in that 0.3 parts of supramolecular carnosine of preparation 3 is replaced by 0.3 parts of carnosine, and other materials, amounts and preparation methods are referred to in example 1.

Comparative example 4

This comparative example provides a multi-effect composition which differs from example 1 only in that no recombinant collagen was added, and other materials, amounts and preparation methods are referred to in example 1.

Comparative example 5

This comparative example provides a multi-effect composition that differs from example 1 only in that no guo pi arsu fruit nanocapsules are added, and other raw materials, amounts and preparation methods are referred to in example 1.

Comparative example 6

This comparative example provides a multi-effect composition which differs from example 1 only in that no supramolecular kapok is added, and other materials, amounts and methods of preparation are referenced in example 1.

Comparative example 7

This comparative example provides a multi-effect composition which differs from example 1 only in that no supramolecular carnosine is added, and other materials, amounts and methods of preparation are referred to in example 1.

Test example 1

Antioxidant test

DPPH radical scavenging ability evaluation: the ability to scavenge DPPH (1, 1-diphenyl-2-trinitrophenylhydrazine) free radical is the antioxidant ability of the reactive species to some extent. The higher the free radical scavenging rate, the stronger the antioxidant capacity and the stronger the anti-aging capacity. Thus, the anti-aging effect of the anti-aging composition can be judged by studying the DPPH radical scavenging ability of the composition.

The compositions prepared in examples and comparative examples were diluted with DMSO to a test sample of 2% by mass, and the following tests were performed:

Adding 2.0mL of DPPH test solution with the mass concentration of 45.8mg/L and a sample to be tested into a test tube, supplementing the total volume to 3mL by absolute ethyl alcohol, shaking uniformly, carrying out light-shielding reaction for 30min, and measuring absorbance at 517nm wavelength by using A1 cm cuvette, and marking as A10; adding 2mL of absolute ethyl alcohol and a sample to be detected with a corresponding volume into a test tube, supplementing the total volume to 3mL by using the absolute ethyl alcohol, and recording the measured absorbance as A11; adding 2mL of DPPH test solution and 1mL of absolute ethyl alcohol into the test tube, recording the measured absorbance as A12, and calculating the clearance (Y') of the solution to be tested to DPPH free radicals;

The calculation formula of the DPPH free radical clearance is as follows:

wherein A ₁₀ is the absorbance value of the system after DPPH is removed by the sample to be detected, A ₁₁ is the absorbance value of the system after DPPH is removed by the sample blank control, and A ₁₂ is the absorbance value of the reaction system before adding the medicine.

The test results are shown in Table 1.

Test example 2

Anti-glycation test

The test was performed using an advanced glycation end product clearance assay, and the following reagents were formulated separately: 0.1mol/LNaOH solution, 0.05mol/L PBS phosphate buffer (pH 7.4), 10mg/mL bovine serum albumin solution, 45mg/mL glucose solution, 0.25mmol/L aminoguanidine hydrochloride solution and 200mg/mL of the examples, comparative example were dissolved in water to obtain a sample solution to be tested.

PBS phosphate buffer solution, bovine serum albumin solution and glucose solution are placed in a constant temperature and humidity incubator, then the sample solution to be tested and aminoguanidine hydrochloride solution are added, and the incubation is carried out for 40 hours at 60 ℃. Taking out each sample solution, detecting the sample solution by using an enzyme-labeled instrument, and recording the fluorescence absorption value, wherein the fluorescence excitation/emission wavelength is 370/440 nm. 3 replicates were made for each sample and the results averaged, wherein AEGs inhibition was calculated using the following formula:

Wherein A _{Sample of} represents the fluorescence intensity of the test substance and the glucose solution; a _{Sample blank} represents the fluorescence intensity with and without the addition of the test substance; a _{Negative control} represents the fluorescence intensity of the glucose solution without the test substance; a _{Negative control for sugar deficiency} represents the fluorescence intensity without the test substance and without the glucose solution.

The test results are shown in Table 1.

Test example 3

Skin stratum corneum moisture content test

Test instrument: skin stratum corneum moisture content analyzer milipure;

test sample: an antioxidant moisturizing water emulsion comprises the following components: 5% of the multi-effect composition prepared in the example and the comparative example, 4% of squalane, 3% of glycerol, 5% of betaine, 2% of hyaluronic acid, 2% of sodium stearoyl lactate, 0.5% of guar gum and the balance of purified water;

Testing: 2 weeks (D ₂), 4 weeks (D ₄);

Test object: 30 healthy women aged 20-45;

Measurement area: the test position is near the temple of the right face of the volunteer;

Interpretation of test parameters:

Skin stratum corneum moisture content: the higher the value, the more moisture the skin's stratum corneum is. Units: comeometer Unit, abbreviated c.u. Moisture increase (%) = (D _n-D₀)×100％/D₀, where Dn may be the moisture content of the subject's skin at D ₂、D₄ and D ₀ is the initial moisture content of the subject's skin.

TABLE 1

According to the table data, when the multiple-effect composition adopts recombinant collagen, guo's-Apostigma nano capsule, supermolecule kapok and supermolecule carnosine to be matched according to a specific proportion, the four raw materials act synergistically to jointly promote the effects of antioxidation, saccharification resistance and moisture preservation, and the effect is optimal.

From examples 1 and 4-5, it is known that the antioxidant, anti-glycation and moisturizing effects are affected when the amount of the ancient apo-su fruit nanocapsules, the supramolecular kapok or the supramolecular carnosine in the composition is too much or too little; from examples 1 and 6, acetone is used as a solvent in the preparation of the gupo-assuo nanocapsules, the activity is best, the bioavailability is high, ethanol is used instead, and the skin care effect is affected to a certain extent.

From example 1 and comparative examples 1 to 3, it is understood that when crude oil of ancient hackberry, crude extract of kapok and crude carnosine are directly used as raw materials, the biological activity of the effective molecules in the multi-effect composition is reduced, and the effects of antioxidation, anti-glycation and moisture preservation are greatly reduced.

From example 1, comparative examples 4-7, it is apparent that the antioxidant, anti-glycation and moisturizing efficacy of the composition is greatly reduced when recombinant collagen, cupola sachalinensis nanocapsules, supramolecular kapok or supramolecular carnosine is arbitrarily absent from the composition.

The applicant states that the process of the invention is illustrated by the above examples, but the invention is not limited to, i.e. it does not mean that the invention has to be carried out in dependence on the above examples. It should be apparent to those skilled in the art that any modification of the present invention, equivalent substitution of raw materials for the product of the present invention, addition of auxiliary components, selection of specific modes, etc., falls within the scope of the present invention and the scope of disclosure.

The preferred embodiments of the present invention have been described in detail above, but the present invention is not limited to the specific details of the above embodiments, and various simple modifications can be made to the technical solution of the present invention within the scope of the technical concept of the present invention, and all the simple modifications belong to the protection scope of the present invention.

In addition, the specific features described in the above embodiments may be combined in any suitable manner, and in order to avoid unnecessary repetition, various possible combinations are not described further.

Claims

1. A multi-effect composition containing recombinant collagen, characterized in that, in parts by weight, the multi-effect composition consists of 0.1-0.5 parts of recombinant collagen, 1-10 parts of cupuacu fruit nanocapsules, 1-10 parts of supramolecular kapok flowers, and 0.1-1 parts of supramolecular carnosine;

The cupuacu fruit nanocapsules are prepared by the following method, which comprises:

(1) dissolving cupuacu oil and pluronic in acetone to obtain a mixed solution;

(2) dropping the mixed solution into distilled water that is being stirred, and continuing to stir, and removing acetone by natural evaporation to obtain nanoparticles;

(3) Adding chitosan to the continuously stirred nanoparticles to obtain the cupuacu fruit nanocapsules.

2. The multi-effect composition according to claim 1 is characterized in that, in parts by weight, the multi-effect composition consists of 0.1-0.5 parts of recombinant collagen, 6-10 parts of cupuacu fruit nanocapsules, 6-10 parts of supramolecular kapok flowers, and 0.1-0.5 parts of supramolecular carnosine.

3. The multi-effect composition according to claim 1, characterized in that the hydrophile-lipophile balance value of the Pluronic is 8-29.

4. The multi-effect composition according to claim 1, characterized in that the molecular weight of the chitosan is 3-20 kDa.

5. The multi-effect composition according to claim 1, characterized in that the mass ratio of the cupuacu oil to the pluronic is 1:(3-5).

6. The multi-effect composition according to claim 1, characterized in that the mass volume ratio of the cupuacu oil and acetone is (4-6) mg:1 mL.

7. The multi-effect composition according to claim 1, characterized in that the amount of distilled water is 4 times that of acetone.

8. The multi-effect composition according to claim 1, characterized in that the supramolecular kapok flower is prepared by the following method, which comprises: extracting kapok flower powder by using supramolecular extracting solution to obtain the supramolecular kapok flower.

9. The multi-effect composition according to claim 8, characterized in that the solid-liquid ratio of the kapok flower powder to the supramolecular extract is 1:(30-70).

10. The multi-effect composition according to claim 8, characterized in that the supramolecular extract comprises betaine, glycerol and propylene glycol.

11. A method for preparing the multi-effect composition containing recombinant collagen as claimed in any one of claims 1 to 10, characterized in that the preparation method comprises: uniformly mixing the recombinant collagen, cupuacu fruit nanocapsules, supramolecular kapok flowers and supramolecular carnosine to obtain the composition.

12. An antioxidant moisturizing lotion, characterized in that the raw materials of the antioxidant moisturizing lotion include: the multi-effect composition containing recombinant collagen according to any one of claims 1 to 10, an emollient, a moisturizer, an emulsifier and a thickener.

13. The antioxidant moisturizing emulsion according to claim 12, characterized in that the mass percentage of the multi-effect composition in the antioxidant moisturizing emulsion is 1-10%.