CN117368500A - Sample analyzer and control method - Google Patents
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- CN117368500A CN117368500A CN202210763507.7A CN202210763507A CN117368500A CN 117368500 A CN117368500 A CN 117368500A CN 202210763507 A CN202210763507 A CN 202210763507A CN 117368500 A CN117368500 A CN 117368500A
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- 238000000034 method Methods 0.000 title claims abstract description 47
- 238000001514 detection method Methods 0.000 claims abstract description 445
- 230000003204 osmotic effect Effects 0.000 claims abstract description 294
- 210000003743 erythrocyte Anatomy 0.000 claims abstract description 249
- 210000004369 blood Anatomy 0.000 claims abstract description 122
- 239000008280 blood Substances 0.000 claims abstract description 122
- 230000001276 controlling effect Effects 0.000 claims abstract description 98
- 238000002331 protein detection Methods 0.000 claims abstract description 90
- 230000000875 corresponding effect Effects 0.000 claims abstract description 46
- 238000011895 specific detection Methods 0.000 claims abstract description 9
- 238000006243 chemical reaction Methods 0.000 claims description 434
- 239000007788 liquid Substances 0.000 claims description 230
- 239000003153 chemical reaction reagent Substances 0.000 claims description 168
- 108090000623 proteins and genes Proteins 0.000 claims description 19
- 102000004169 proteins and genes Human genes 0.000 claims description 19
- 238000004891 communication Methods 0.000 claims description 11
- 238000005406 washing Methods 0.000 claims description 6
- 238000005138 cryopreservation Methods 0.000 claims description 4
- 239000000523 sample Substances 0.000 description 227
- 238000012360 testing method Methods 0.000 description 35
- 238000004140 cleaning Methods 0.000 description 22
- 239000003795 chemical substances by application Substances 0.000 description 19
- 238000010586 diagram Methods 0.000 description 10
- 210000000265 leukocyte Anatomy 0.000 description 10
- 210000004027 cell Anatomy 0.000 description 9
- 239000000243 solution Substances 0.000 description 8
- 238000012546 transfer Methods 0.000 description 8
- 239000012530 fluid Substances 0.000 description 6
- 239000012085 test solution Substances 0.000 description 6
- 108010054147 Hemoglobins Proteins 0.000 description 5
- 102000001554 Hemoglobins Human genes 0.000 description 5
- 239000003085 diluting agent Substances 0.000 description 5
- 230000008595 infiltration Effects 0.000 description 5
- 238000001764 infiltration Methods 0.000 description 5
- 210000003651 basophil Anatomy 0.000 description 4
- 239000003219 hemolytic agent Substances 0.000 description 4
- 239000004816 latex Substances 0.000 description 4
- 229920000126 latex Polymers 0.000 description 4
- 238000004458 analytical method Methods 0.000 description 3
- 210000001772 blood platelet Anatomy 0.000 description 3
- 230000002949 hemolytic effect Effects 0.000 description 3
- 239000000815 hypotonic solution Substances 0.000 description 3
- 230000002035 prolonged effect Effects 0.000 description 3
- 238000009666 routine test Methods 0.000 description 3
- 238000010998 test method Methods 0.000 description 3
- 206010018910 Haemolysis Diseases 0.000 description 2
- 238000010521 absorption reaction Methods 0.000 description 2
- 238000004820 blood count Methods 0.000 description 2
- 210000000170 cell membrane Anatomy 0.000 description 2
- HVYWMOMLDIMFJA-DPAQBDIFSA-N cholesterol Chemical compound C1C=C2C[C@@H](O)CC[C@]2(C)[C@@H]2[C@@H]1[C@@H]1CC[C@H]([C@H](C)CCCC(C)C)[C@@]1(C)CC2 HVYWMOMLDIMFJA-DPAQBDIFSA-N 0.000 description 2
- 238000004590 computer program Methods 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 210000003617 erythrocyte membrane Anatomy 0.000 description 2
- 230000008588 hemolysis Effects 0.000 description 2
- 230000031700 light absorption Effects 0.000 description 2
- 238000005259 measurement Methods 0.000 description 2
- 238000012545 processing Methods 0.000 description 2
- 238000010186 staining Methods 0.000 description 2
- 208000015710 Iron-Deficiency Anemia Diseases 0.000 description 1
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- 208000036696 Microcytic anaemia Diseases 0.000 description 1
- 208000002903 Thalassemia Diseases 0.000 description 1
- 238000003491 array Methods 0.000 description 1
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- 210000001124 body fluid Anatomy 0.000 description 1
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- 235000012000 cholesterol Nutrition 0.000 description 1
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Classifications
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
- G01N35/00584—Control arrangements for automatic analysers
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
- G01N35/10—Devices for transferring samples or any liquids to, in, or from, the analysis apparatus, e.g. suction devices, injection devices
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- Physics & Mathematics (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Investigating Or Analysing Biological Materials (AREA)
Abstract
The embodiment of the application discloses a sample analyzer and a control method, wherein the method is applied to the sample analyzer and comprises the following steps: and determining the working mode of the sample analyzer, and controlling a functional module of the sample analyzer to execute corresponding actions according to the working mode so as to realize specific item detection, wherein the functional module at least comprises a dispensing device and a detection device, and the specific detection item comprises at least one of specific protein detection and blood routine detection and erythrocyte osmotic fragility detection. The control method provided by the embodiment of the application can be realized, the sample analyzer can not only carry out erythrocyte osmotic fragility detection on the sample, but also realize detection of at least one of specific protein detection and blood routine detection, compared with the separate detection in the prior art, the detection efficiency is improved, the detection cost is reduced, the detection workload is reduced, and the time required by sample detection is saved.
Description
Technical Field
The present application relates to the field of medical devices, and in particular, to a sample analyzer and a control method.
Background
Erythrocyte osmotic fragility, i.e. the tensile strength of erythrocyte membranes in hypotonic solutions, and the decrease of membrane cholesterol may be the root cause of the increase of cell membrane fragility, and the measurement of erythrocyte osmotic fragility is an important reference index in clinic for microcytic anaemia such as thalassemia, iron deficiency anaemia, etc., but if a sample is only subjected to erythrocyte osmotic fragility detection, fewer reference indexes will appear, thus leading to inaccurate detection results.
In the prior art, different detection instruments are generally adopted to separately detect samples, such as specific protein detection and erythrocyte osmotic fragility detection, and comprehensive analysis is performed on detection results of the specific protein detection and the erythrocyte osmotic fragility detection, so that the accuracy of the detection results is improved, but the mode of separate detection is high in cost and high in detection workload.
Disclosure of Invention
The embodiment of the application mainly aims to provide a sample analyzer and a control method, and aims to reduce detection cost and detection workload.
In a first aspect, embodiments of the present application provide a sample analyzer, comprising:
a dispensing device for transferring a sample and/or a reagent;
a reaction device having at least two reaction parts for providing reaction sites for the samples and/or the reagents transferred by the dispensing device, so that the samples and the corresponding reagents in the corresponding reaction parts are mixed to form a sample liquid to be tested, wherein the sample liquid to be tested comprises at least one of a specific protein detection liquid and a blood routine detection liquid, and a red blood cell permeation fragility detection liquid;
a detection device for detecting a specific protein in the specific protein detection liquid and/or detecting a blood routine in the blood routine detection liquid in the reaction part, and detecting a red blood cell osmotic fragility in the red blood cell osmotic fragility detection liquid in the reaction part;
Control means, in communication with said dispensing means and said detecting means, for:
determining a working mode of the sample analyzer, and controlling a functional module of the sample analyzer to execute corresponding actions according to the working mode so as to realize specific item detection, wherein the functional module at least comprises the dispensing device and the detection device, and the specific detection item comprises at least one of specific protein detection and blood routine detection, and erythrocyte osmotic fragility detection.
In a second aspect, embodiments of the present application provide a control method applied to a sample analyzer, where the sample analyzer includes a dispensing device, a reaction device, and a detection device; the dispensing device is used for transferring samples and/or reagents; a reaction device having at least two reaction parts for providing reaction sites for the samples and/or the reagents transferred by the dispensing device, so that the samples and reagents in the corresponding reaction parts are mixed to form a sample liquid to be tested, wherein the sample liquid to be tested comprises at least one of a specific protein detection liquid and a blood routine detection liquid, and a red blood cell permeation fragility detection liquid; the detection device is used for carrying out specific protein detection on the specific protein detection liquid in the reaction part and/or carrying out blood routine detection on the blood routine detection liquid, and carrying out red blood cell osmotic fragility detection on the red blood cell osmotic fragility detection liquid in the reaction part;
The method comprises the following steps: determining a working mode of the sample analyzer, and controlling a functional module of the sample analyzer to execute corresponding actions according to the working mode so as to realize specific item detection, wherein the functional module at least comprises the dispensing device and the detection device, and the specific detection item comprises at least one of specific protein detection and blood routine detection, and erythrocyte osmotic fragility detection.
The application provides a sample analyzer and a control method, wherein the sample analyzer comprises a dispensing device, a reaction device, a detection device and a control device, wherein the dispensing device is used for transferring samples and/or reagents; the reaction device is provided with at least two reaction parts, wherein the reaction parts are used for providing reaction places for the samples and/or the reagents transferred by the dispensing device so as to enable the samples and the corresponding reagents in the corresponding reaction parts to be mixed to form a sample liquid to be tested, and the sample liquid to be tested comprises at least one of a specific protein detection liquid and a blood routine detection liquid and a red blood cell permeation fragility detection liquid; the detection device is used for carrying out specific protein detection on the specific protein detection liquid in the reaction part and/or carrying out blood routine detection on the blood routine detection liquid, and carrying out red blood cell osmotic fragility detection on the red blood cell osmotic fragility detection liquid in the reaction part; the control device is in communication connection with the dispensing device and the detecting device and is used for: determining a working mode of the sample analyzer, and controlling a functional module of the sample analyzer to execute corresponding actions according to the working mode so as to realize specific item detection, wherein the functional module at least comprises the dispensing device and the detection device, and the specific detection item comprises at least one of specific protein detection and blood routine detection, and erythrocyte osmotic fragility detection. In this embodiment, the sample analyzer is provided with at least two kinds of mode, under different mode, the sample analyzer can carry out corresponding specific project and detect to this specific detection project includes at least one of specific protein detection and blood routine detection, and red blood cell infiltration friability detects, namely, this sample analyzer both can carry out red blood cell infiltration friability detection to the sample, can realize the detection of at least one of specific protein detection and blood routine detection again, compare in prior art's separately detects, detection cost has been reduced when improving detection efficiency, detection work load has been reduced, and sample detection required time has been practiced thrift.
It is to be understood that both the foregoing general description and the following detailed description are exemplary and explanatory only and are not restrictive of the disclosure of embodiments of the present application.
Drawings
In order to more clearly illustrate the technical solutions of the embodiments of the present application, the drawings needed in the description of the embodiments will be briefly introduced below, and it is obvious that the drawings in the following description are some embodiments of the present application, and other drawings may be obtained according to these drawings without inventive effort for a person skilled in the art.
FIG. 1 is a schematic block diagram of a sample analyzer according to an embodiment of the present application;
FIG. 2 is a schematic structural view of a dispensing device of a sample analyzer according to an embodiment of the present application;
FIGS. 3-4 are timing diagrams of a sample analyzer in a first mode of operation according to embodiments of the present application;
FIGS. 5-7 are timing diagrams of the operation of the sample analyzer in the second operation mode according to the embodiments of the present application;
fig. 8 is a flowchart of steps of a control method of a sample analyzer according to an embodiment of the present application.
Detailed Description
The following description of the embodiments of the present application will be made clearly and fully with reference to the accompanying drawings, in which it is evident that the embodiments described are some, but not all, of the embodiments of the present application. All other embodiments, which can be made by one of ordinary skill in the art without undue burden from the present disclosure, are within the scope of the present disclosure.
In the description of the present application, unless explicitly stated and limited otherwise, the terms "mounted," "connected," and "connected" are to be construed broadly, and may be, for example, fixedly connected, detachably connected, or integrally connected; can be mechanically or electrically connected; can be directly connected or indirectly connected through an intermediate medium, and can be communication between two elements. The specific meaning of the terms in this application will be understood by those of ordinary skill in the art in a specific context.
The flow diagrams depicted in the figures are merely illustrative and not necessarily all of the elements and operations/steps are included or performed in the order described. For example, some operations/steps may be further divided, combined, or partially combined, so that the order of actual execution may be changed according to actual situations.
Some embodiments of the present application will be described in detail below with reference to the accompanying drawings, and the following examples and features of the examples may be combined with each other without conflict.
Referring to fig. 1, a sample analyzer 100 is provided for analyzing a sample to be tested to obtain a corresponding analysis result.
As shown in fig. 1, the sample analyzer 100 includes a sample supply device 10, a dispensing device 20, a reagent supply device 30, a reaction device 40, a detection device 60, and a control device 70.
The sample supply device 20 is used for providing a sample to be detected, the reagent supply device 30 is used for providing a reagent reacting with the sample, the dispensing device 20 is used for transferring the sample and/or the reagent, the reaction device 40 is provided with at least two reaction parts 401, and the reaction parts 401 are used for providing reaction sites for the sample and/or the reagent transferred by the dispensing device 20 so as to mix the sample and the reagent in the corresponding reaction parts 401 to form a sample liquid to be detected.
Wherein the sample to be tested includes, but is not limited to, blood samples, and the reagents include, but are not limited to, hemolysis agents, diluents, latex reagents, erythrocyte osmotic fragility detection reagents, and the like.
The detection device 60 is configured to detect the sample liquid to be detected in the reaction portion 401, so as to obtain a corresponding detection result. Illustratively, the test sample fluid includes at least one of a specific protein test fluid and a blood routine test fluid, and a red blood cell osmotic fragility test fluid. The detection device 60 detects a specific protein in the specific protein detection liquid and/or a blood routine in the reaction part 401, and detects a red blood cell osmotic fragility in the red blood cell osmotic fragility detection liquid in the reaction part 401. That is, when the detection device 60 detects a specific protein in the specific protein detection liquid in the reaction portion 401, a specific protein detection result of the sample to be measured can be obtained, when the detection device 60 detects a blood routine in the specific protein detection liquid in the reaction portion 401, a blood routine detection result of the sample to be measured can be obtained, and when the detection device 60 detects a red blood cell osmotic fragility in the red blood cell osmotic fragility detection liquid in the reaction portion 401, a red blood cell osmotic fragility detection result of the sample to be measured can be obtained.
Referring to fig. 2, in some embodiments, the dispensing device 20 includes a pipette needle 201, a driving assembly 202, and a pipette driving part 203, wherein the driving assembly 202 is used for supporting the pipette needle 201 and driving the pipette needle 201 to move. For example, the pipetting needle 201 is moved in two or three dimensions in space by a two or three dimensional drive assembly 202, whereby the pipetting needle 201 can be moved to aspirate samples carried by the sample supply 20 and/or reagents supplied by the reagent supply 30.
The pipetting driving unit 203 is configured to quantitatively aspirate a sample through the pipetting needle 201, for example, the pipetting needle 201 is moved into a sample tube loaded with a blood sample on the sample supply device 20 under the driving of the driving unit 202, aspirate the blood sample to be measured under the driving of the pipetting driving unit 203, and convey the blood sample to be measured into the reaction unit 401 of the reaction device 40, so that the blood sample to be measured aspirated by the dispensing device 20 and a reagent provided by the reagent supply device 20 are mixed in the reaction unit 401 to prepare a sample to be measured, which is also referred to as a sample solution to be measured.
In some embodiments, the pipetting drive unit 203 comprises a tube 2031 and a power unit 2033, wherein the tube 2031 is used for transporting a fluid medium, one end of the tube 2031 is communicated with the pipetting needle 201, and the other end is communicated with the power unit 2033, so that the flow direction of the fluid medium in the tube 2031 is changed by the power unit 2033, and the pipetting needle 201 can transfer samples and/or reagents.
It will be appreciated that depending on the body fluid to be tested and the item to be tested, the sample and the reagent may be added in different ways, for example, the sample and the reagent may be added by using the pipetting needle 201, or the sample may be added by using the pipetting needle 201, the reagent may be added by using the reagent needle, or only the sample may be added by using the pipetting needle 201, or the reagent may be added by other ways, for example, the reagent may be connected to a reagent container carrying the reagent by a dedicated line to add the reagent to the reaction part 401.
In some embodiments, the sample analyzer 100 further comprises a cryopreservation device 60, the cryopreservation device 60 for cryopreserving reagents, wherein the reagents include, but are not limited to, at least one of a specific protein reagent and a red blood cell permeable fragility reagent.
Illustratively, the specific protein reagent includes a hemolyzing agent and a latex reagent, it being understood that the temperature of the latex reagent needs to be stabilized at a specific value to ensure that the activity of the reagent is maintained, and thus the specific protein reagent needs to be stored in the refrigerator 60 at a time other than the time of sucking and filling the latex reagent.
Meanwhile, in order to fully utilize the space of the refrigerator 60, a portion of the erythrocyte permeable fragile reagent is placed in the refrigerator 60 to reduce the volume of the sample analyzer.
In some embodiments, the sample analyzer 100 further comprises a cleaning device for cleaning target devices, including but not limited to the pipetting needle 201 and the reaction portion 401.
In some embodiments, the control device 70 includes at least a processor 701, a memory 702, a communication interface (not shown), and an I/O interface (not shown). The processor 701, memory 702, communication interfaces, and I/O interfaces communicate over a bus. The processor 701 may be a central processing unit (Central Processing Unit, CPU) which may also be other general purpose processors, digital signal processors (Digital Signal Processor, DSP), application specific integrated circuits (Application Specific Integrated Circuit, ASIC), field-programmable gate arrays (Field-Programmable Gate Array, FPGA) or other programmable logic devices, discrete gate or transistor logic devices, discrete hardware components, or the like. Wherein the general purpose processor may be a microprocessor or the processor may be any conventional processor or the like.
Various computer programs to be executed by the processor 701, such as an operating system and application programs, are stored in the memory 702, and data necessary for executing the computer programs. During the course of the test analysis of a particular test item, data stored locally, if desired, may be stored in the memory 702. The I/O interface includes, but is not limited to, serial interfaces such as USB, IEE394 or RS-232C, parallel interfaces such as SCSI, IDE or IE84, and analog signal interfaces composed of D/a converters, and the like. An input device is coupled to the I/O interface and a user may input data directly to the control device 70 using the input device, including but not limited to a keyboard, mouse, touch screen, or control buttons. The display device may be communicatively coupled to the control device 70 via an I/O interface for relevant information presentation. The communication interface may be any communication protocol known so far, and the communication interface communicates with the outside through a network, and the control device 70 may transmit data between any device connected through the network through the communication interface in a preset communication protocol.
In some embodiments, the control device 70 is configured to:
the operation mode of the sample analyzer 100 is determined, and the functional module of the sample analyzer 100 is controlled to perform corresponding actions according to the operation mode to realize specific item detection, wherein the functional module at least comprises a dispensing device 20 and a detection device 50, and the specific detection item comprises at least one of specific protein detection and blood routine detection, and erythrocyte osmotic fragility detection.
Illustratively, the sample analyzer 100 is preconfigured with at least two modes of operation, and in different modes of operation, the control device 70 controls the dispensing device 20 and the detection device 50 to perform different corresponding actions to effect detection of different specific items.
Illustratively, the operation modes include a first operation mode, the reaction part 401 includes a first reaction part, a second reaction part, and a third reaction part, and the control device 70 includes, in controlling the functional module of the sample analyzer 100 to perform corresponding actions according to the operation modes to implement a specific item detection process:
in a state in which the sample analyzer 100 is in the first operation mode, the control device 70 controls the dispensing device 20 to respectively fill the first reaction part, the second reaction part, and the third reaction part with the sample and/or the corresponding reagent to form the erythrocyte osmotic fragility detection liquid in the first reaction part, the specific protein detection liquid in the second reaction part, and the blood routine detection liquid in the third reaction part;
The control detecting device 50 detects the erythrocyte osmotic fragility of the erythrocyte osmotic fragility detecting liquid of the first reaction part, detects the specific protein of the specific protein detecting liquid of the second reaction part, and detects the blood routine of the blood routine detecting liquid in the third reaction part.
It should be appreciated that when the sample analyzer 100 is in the first operation mode, the sample analyzer 100 is at least used for performing erythrocyte osmotic fragility detection, specific protein detection and blood routine detection on a sample to be detected.
Specifically, in a state in which the sample analyzer 100 is in the first operation mode, the control device 70 controls the dispensing device 20 to fill the first reaction part, the second reaction part, and the third reaction part with the sample and/or the corresponding reagent, respectively, to form the erythrocyte osmotic fragility detection liquid in the first reaction part, to form the specific protein detection liquid in the second reaction part, and to form the blood regular detection liquid in the third reaction part. Wherein the first reaction part, the second reaction part and the third reaction part are separately arranged. After the corresponding detection solutions are formed in the first reaction part, the second reaction part and the third reaction part, respectively, the control device 70 controls the detection device 50 to perform the erythrocyte osmotic fragility detection on the erythrocyte osmotic fragility detection solution of the first reaction part, to perform the specific protein detection on the specific protein detection solution of the second reaction part and to perform the blood routine detection on the blood routine detection solution in the third reaction part.
By providing the first, second and third reaction units in the reaction device, and filling the respective reaction units with the sample and/or the corresponding reagent by the dispensing device 20, and performing the corresponding detection operations on the detection liquids in the first, second and third reaction units, respectively, the sample analyzer 100 can efficiently perform erythrocyte osmotic fragility detection, specific protein detection and blood routine detection on the sample. Compared with the separate detection in the prior art, the method has the advantages that the detection efficiency is improved, the detection cost is reduced, the detection workload is reduced, and the time required by sample detection is saved.
It is understood that each of the first, second and third reaction sections includes at least one reaction vessel, such as a reaction cell, such that the sample and reagents are mixed within the reaction cell to form a reaction solution.
It is also understood that blood routine tests include, but are not limited to, blood five-class, white blood cell count, hemoglobin concentration tests. In the case of five-stage Blood sorting, there may be a plurality of reaction vessels corresponding to the reaction unit, for example, 5 reaction vessels, namely, DIFF (Differential) Cell, WBC (White Blood Cell), HGB (Hemoglobin) Cell, RBC (Red Blood Cell) Cell, and RINSE (flushing) Cell. The DIFF cell is used for four classification of white blood cells by adopting a light absorption method and an electrical impedance method: the classifying hemolytic agent dissolves erythrocytes and stains leukocytes, after a period of time, the staining reaction is terminated by a subsequently added diluent, and the instrument distinguishes four groups of lymphocytes, monocytes, eosinophils and neutrophils by measuring the volume and light absorption value of the leukocytes. WBC pools were used for white blood cell count and basophil classification: the blood sample is mixed with a certain amount of basophilic hemolytic agent, erythrocytes are dissolved by the hemolytic agent, other leukocytes except basophils are significantly contracted, and basophils exist as large cells, and the basophils are distinguished from other leukocytes by measuring the volume (impedance method). HGB cell was used for hemoglobin measurement: the surface active agent in the haemoglobin hemolytic agent breaks the cell membrane of the red blood cells, so that the haemoglobin is released and oxidized into methemoglobin, and forms a stable derivative with the haemolytic agent, the derivative has maximum absorption performance at the wavelength of 540nm, and the instrument can calculate the content of the haemoglobin by detecting the absorption peak value. RBC pools were used for RBC (Red Blood Cell) and PLT (platelets) counts: the diluent is a buffer solution with certain conductivity, can dilute a blood sample, and can keep the original form within a certain time after the blood cells are diluted, so that the diluent can be used for counting red blood cells, platelets and the like. The RINSE pool is used for waste liquid treatment, needle cleaning and the like.
In some embodiments, the control device 70 is further configured to:
in a state in which the sample analyzer 100 is in the first operation mode, the dispensing device 20 is controlled to add the first osmotic fragility detection reagent to the first reaction part;
after the addition of the first osmotic fragility detection reagent is completed, controlling the dispensing device 20 to draw the sample into the sample container and add the sample into the first reaction part carrying the first osmotic fragility detection reagent so as to form a first erythrocyte osmotic fragility detection liquid in the first reaction part; and controlling the detecting means 50 to perform a first detecting operation of the erythrocyte osmotic fragility on the first erythrocyte osmotic fragility detecting liquid in the first reaction part;
in the process of performing the first detection operation of erythrocyte osmotic fragility, controlling the dispensing device 20 to add a sample to the third reaction part so as to form a blood routine detection liquid in the third reaction part, and controlling the detecting device 50 to perform a blood routine detection operation on the blood routine detection liquid in the third reaction part;
after the completion of the operation of adding the sample to the third reaction part, the dispensing device 20 is controlled to add the sample to the second reaction part carrying the first specific protein detection reagent;
after the operation of adding the sample to the second reaction part is completed and after the first detection operation of erythrocyte fragility is completed, controlling the dispensing device 20 to add the sample to the first reaction part carrying the second osmotic fragility detection reagent so as to form a second erythrocyte osmotic fragility detection liquid in the first reaction part; and controls the detecting device 50 to perform a second detecting operation of the erythrocyte osmotic fragility on the second erythrocyte osmotic fragility detecting liquid in the first reaction part;
During execution of the erythrocyte osmotic fragility second detection operation, the dispensing device 20 is controlled to add the second specific protein detection reagent to the second reaction part carrying the mixed liquid of the first specific protein detection reagent and the sample so that the specific protein detection liquid is formed in the second reaction part; and controls the detection device 50 to detect a specific protein in the specific protein detection liquid in the second reaction part. Referring to fig. 3, fig. 3 is a timing diagram of a workflow of the sample analyzer 100 in the first operation mode according to the embodiment of the present application.
For ease of illustration in the relevant timing diagrams of embodiments of the present application, E1 represents a first osmotic fragility detection reagent; e2 represents a second osmotic fragility detection reagent; r1 represents a first specific protein detection reagent; r2 a second specific protein detection reagent; s represents a sample, and in the present embodiment, a blood sample is taken as an example. Further, it is easy to recognize the execution operation of the dispensing device 20 in the entire timing chart, and the timing portion executed by the dispensing device 20 is displayed in a gray scale pattern.
In this embodiment, the erythrocyte osmotic fragility is measured by a scattering nephelometry, that is, the ratio between the difference of the scattered light of the reaction cup after the same sample is treated by the first osmotic fragility detection reagent and the difference of the scattered light of the reaction cup after the same sample is treated by the second osmotic fragility detection reagent is calculated, so that the hemolysis rate of the sample, that is, erythrocyte fragility detection data can be obtained.
Specifically, in a working procedure sequence of the sample analyzer 100 in the first working mode, the control device 70 first controls the dispensing device 20 to add the first osmotic fragility detection reagent to the first reaction part, then after the addition of the first osmotic fragility detection reagent is completed, controls the dispensing device 20 to draw the sample to be detected from the preset sample container, and adds the sample to the first reaction part carrying the first osmotic fragility detection reagent, so that the sample is mixed with the first osmotic fragility detection reagent in the first reaction part to form the first erythrocyte osmotic fragility detection liquid. After the first erythrocyte osmotic fragility test solution is formed in the first reaction part, the control device 70 controls the test device 50 to perform the erythrocyte osmotic fragility first test operation on the first erythrocyte osmotic fragility test solution in the first reaction part.
In the process of the detecting device 50 performing the first detecting operation of the erythrocyte osmotic fragility, the control device 70 controls the dispensing device 20 to add the sample to the third reaction part so that the blood routine detecting liquid is formed in the third reaction part, and controls the detecting device 50 to perform the blood routine detecting operation on the blood routine detecting liquid in the third reaction part. After the addition of the sample to the third reaction section is completed, the control device 70 controls the dispensing device 20 to add the sample to the second reaction section carrying the first specific protein detection reagent. After the operation of adding the sample to the second reaction part is completed and after the first detection operation of erythrocyte fragility is completed, the control device 70 controls the dispensing device 20 to add the sample to the first reaction part carrying the second osmotic fragility detection reagent so that the second erythrocyte osmotic fragility detection liquid is formed in the first reaction part, and controls the detection device 50 to perform the second detection operation of erythrocyte osmotic fragility on the second erythrocyte osmotic fragility detection liquid in the first reaction part. During the execution of the second detection operation of erythrocyte osmotic fragility, the control device 70 controls the dispensing device 20 to add the second specific protein detection reagent to the second reaction part carrying the mixed liquid of the first specific protein detection reagent and the sample so that the specific protein detection liquid is formed in the second reaction part; and controls the detection device 50 to perform a specific protein detection operation on the specific protein detection liquid in the second reaction part.
Optionally, the first specific protein detection reagent is added to the second reaction section through a conduit.
Optionally, a second osmotic fragility test agent is added to the first reaction section via a line.
Illustratively, the first osmotic fragility test agent and the second osmotic fragility test agent may be pre-configured hypotonic solutions of different concentrations, and the tensile strength of the erythrocyte membrane in the hypotonic solutions of different concentrations may be detected by mixing the sample with the first osmotic fragility test agent, mixing the sample with the second osmotic fragility test agent, and performing the corresponding test operations.
It is known that after the first detection operation of the erythrocyte osmotic fragility and the second detection operation of the erythrocyte osmotic fragility of the sample are completed, the detection result of the erythrocyte osmotic fragility can be obtained according to the detection results obtained by the two detection operations.
The control device 70 controls the dispensing device 20 and the detecting device 50 to sequentially complete the steps, so that the dispensing device and the detecting device 50 work in a highly matched mode, the detection efficiency of completing erythrocyte osmotic fragility detection, specific protein detection and blood routine detection of a sample to be detected is improved, the detection cost is reduced, the detection workload is reduced, and the time required by sample detection is saved.
It will be appreciated that the pipetting needle 201 of the dispensing device 20 for performing sample and/or reagent movement is the same pipetting needle, i.e. when the pipetting needle 201 of the dispensing device 20 is used for performing both sample movement and reagent movement, the pipetting needle 201 needs to perform a cleaning operation before performing different transfer tasks to ensure accuracy of the detection result, e.g. after performing sample movement, if reagent movement is to be performed by the pipetting needle 201, the needle cleaning operation is performed before performing reagent movement; alternatively, after reagent transfer is performed by the pipette needle 201, if sample transfer is to be performed, a needle cleaning operation is performed; alternatively, after the first reagent transfer is performed, the pipette needle 201 performs a needle cleaning operation if the second reagent transfer is to be performed; alternatively, after the first sample transfer, the pipette needle 201 may perform a needle cleaning operation if the second sample transfer is to be performed. It is also understood that the reaction part is required to be cleaned to ensure the accuracy of the detection result if the second reaction solution is to be continuously mixed after the first reaction solution is mixed. For example, after the first reaction part is formed with the first erythrocyte osmotic fragility test solution, if the reagent and the sample are required to be added to form the second erythrocyte osmotic fragility test solution, the first reaction part is required to be cleaned.
For example, the sample analyzer 100 further includes, before the control device 70 controls the dispensing device 20 to add a sample to the second reaction section carrying the first specific protein detection reagent: the first specific protein detection reagent may be added to the second reaction part, and specifically, the first specific protein detection reagent may be added by the control device 70 controlling the dispensing device 20, or may be added by another device in the sample analyzer 100.
For example, the control device 70 may further include, before controlling the dispensing device 20 to add the sample to the first reaction section carrying the second osmotic fragility detection reagent: the cleaning device is controlled to clean the first reaction part so as to discharge the first erythrocyte osmotic fragility detection liquid in the first reaction part. Thereby avoiding the pollution of the first erythrocyte osmotic fragility detection liquid to the second erythrocyte osmotic fragility detection operation.
For example, the control device 70 further includes, after controlling the detecting device 50 to perform the second detecting operation of the erythrocyte osmotic fragility of the second erythrocyte osmotic fragility detecting liquid in the first reaction part: the cleaning device is controlled to clean the first reaction part and the pipetting needle so as to discharge the second erythrocyte osmotic fragility detection liquid in the first reaction part.
Similarly, in some embodiments, the control device 70 further includes, after controlling the detection device 50 to perform a specific protein detection operation on the specific protein detection liquid in the second reaction portion: the cleaning device is controlled to clean the second reaction part and the pipetting needle so as to discharge the specific protein detection liquid in the second reaction part.
It should be understood that, after the detection operation is completed, the pipetting needle and the corresponding reaction portion are cleaned by the cleaning device, so that the residual detection liquid is prevented from being remained in the reaction device 40 and the dispensing device 20 for a long time, which results in corrosion of the reaction device 40 and the dispensing device 20, and the service life of the sample analyzer 100 is prolonged.
It will be appreciated that when the dispensing device 20 is used only to add sample to the reaction portion 40, it may be used to draw a sufficient amount of sample into the sample tube at one time, thereby allowing for detection of at least two different items for one sample collection, and saving sample collection procedures.
In some embodiments, control device 70, in controlling dispensing device 20 to add a first osmotic fragility test agent to a first reaction site, comprises:
controlling the dispensing device 20 to add a first amount of a first osmotic fragility-detecting reagent to the first reaction part to perform a first washing operation on the first reaction part;
After the first washing operation is completed, the dispensing device 20 is controlled to add a second amount of the first osmotic fragility-detecting reagent to the first reaction part.
Specifically, the control device 70 first controls the dispensing device 20 to add a first amount of the first osmotic fragility test agent to the first reaction part in controlling the addition of the first osmotic fragility test agent to perform a first washing operation on the first reaction part. Then, after the first washing operation is completed, the control device 70 controls the dispensing device 20 to add the second amount of the first osmotic fragility-detecting agent to the first reaction part again.
It should be understood that, the first osmotic fragility detection reagent is added to the first reaction part in a first amount, so as to clean the first reaction part, so as to avoid the original impurities in the first reaction part from polluting the first osmotic fragility detection liquid, and after the cleaning of the first reaction part is completed, the control device 70 controls the dispensing device 20 to add the first osmotic fragility detection reagent to the first reaction part in a second amount, so that the first osmotic fragility detection reagent in the second amount is mixed with the subsequently added sample to form the first osmotic fragility detection reagent, and the detection result obtained by the first osmotic fragility detection operation is more accurate. Optionally, the second amount is greater than the first amount.
Referring to fig. 4, in some embodiments, the control device 70 is further configured to:
in a state in which the sample analyzer 100 is in the first operation mode, controlling the dispensing device 20 to suck the sample into the sample container, and after the sample sucking operation is completed, controlling the dispensing device 20 to add the sample into the first reaction portion carrying the first osmotic fragility detection reagent so as to form a first erythrocyte osmotic fragility detection liquid in the first reaction portion; and controlling the detecting means 50 to perform a first detecting operation of the erythrocyte osmotic fragility on the first erythrocyte osmotic fragility detecting liquid in the first reaction part;
in the process of performing the first detection operation of erythrocyte osmotic fragility, controlling the dispensing device 20 to add a sample to the third reaction part so as to form a blood routine detection liquid in the third reaction part, and controlling the detecting device 50 to perform a blood routine detection operation on the blood routine detection liquid in the third reaction part;
after the execution of the operation of adding the sample to the third reaction part is completed, the dispensing device 20 is controlled to add the sample to the second reaction part carrying the first specific protein detection reagent;
after the operation of adding the sample to the second reaction part is completed, controlling the dispensing device 20 to add the sample to the first reaction part carrying the second osmotic fragility detection reagent so that the second erythrocyte osmotic fragility detection liquid is formed in the first reaction part; and controls the detecting device 50 to perform a second detecting operation of the erythrocyte osmotic fragility on the second erythrocyte osmotic fragility detecting liquid in the first reaction part;
During execution of the erythrocyte osmotic fragility second detection operation, the dispensing device 20 is controlled to add the second specific protein detection reagent to the second reaction part carrying the mixed liquid of the first specific protein detection reagent and the sample so that the specific protein detection liquid is formed in the second reaction part; and controls the detection device 50 to detect a specific protein in the specific protein detection liquid in the second reaction part.
Alternatively, both the first osmotic fragility test agent and the second osmotic fragility test agent are added to the first reaction part through a pipe, and the first specific protein test agent is added to the second reaction part through a pipe.
Fig. 4 is a timing diagram of another workflow of the sample analyzer 100 in the first operation mode according to the embodiment of the present application.
As shown in fig. 4, specifically, in another operational sequence in which the sample analyzer 100 is in the first operational mode, the control device 70 first controls the dispensing device 20 to aspirate the sample to be tested from the sample container, and after the sample aspiration operation is completed, controls the dispensing device 20 to add the sample to the first reaction portion carrying the first osmotic fragility test reagent so that the first erythrocyte osmotic fragility test solution is formed in the first reaction portion, and then controls the testing device 50 to perform the first erythrocyte osmotic fragility test operation on the first erythrocyte osmotic fragility test solution in the first reaction portion. Meanwhile, in performing the first detection operation of erythrocyte osmotic fragility, the control device 70 controls the dispensing device 20 to add a sample to the third reaction part carrying the blood routine detection reagent so that the blood routine detection liquid is formed in the third reaction part, and controls the detection device 50 to perform the blood routine detection operation on the blood routine detection liquid in the third reaction part. Among these, blood routine test reagents include, but are not limited to, diluents, hemolyzing agents, staining agents.
After the execution of the operation of adding the sample to the third reaction part is completed, the control device 70 controls the dispensing device 20 to add the sample to the second reaction part carrying the first specific protein detection reagent, and after the completion of the operation of adding the sample to the second reaction part, the control device 70 controls the dispensing device 20 to add the sample to the first reaction part carrying the second osmotic fragility detection reagent so that the second erythrocyte osmotic fragility detection liquid is formed in the first reaction part, and then controls the detection device 50 to execute the erythrocyte osmotic fragility second detection operation on the second erythrocyte osmotic fragility detection liquid in the first reaction part. In performing the second detection operation of erythrocyte osmotic fragility, the control device 70 also controls the dispensing device 20 to add the second specific protein detection reagent to the second reaction part carrying the mixed liquid of the first specific protein detection reagent and the sample, so that the specific protein detection liquid is formed in the second reaction part, and controls the detection device 50 to perform the specific protein detection on the specific protein detection liquid in the second reaction part.
The control device 70 controls the dispensing device 20 and the detecting device 50 to sequentially complete the steps, so that the dispensing device and the detecting device 50 work in a highly matched mode, the detecting efficiency of erythrocyte osmotic fragility detection, specific protein detection and blood routine detection on a sample is improved, the detecting cost is reduced, the detecting workload is reduced, and the time required by sample detection is saved.
In some embodiments, the control device 70, prior to controlling the dispensing device 20 to add the sample to the first reaction portion carrying the second osmotic fragility detection reagent, further comprises: the cleaning device is controlled to clean the first reaction part so as to discharge the first erythrocyte osmotic fragility detection liquid in the first reaction part. Thereby avoiding the pollution of the first erythrocyte osmotic fragility detection liquid to the second erythrocyte osmotic fragility detection operation.
In some embodiments, the control device 70 further includes, after controlling the detecting device 50 to perform the first detecting operation of the erythrocyte osmotic fragility detecting liquid in the first reaction part: the cleaning device is controlled to clean the first reaction part and the pipetting needle so as to discharge the second erythrocyte osmotic fragility detection liquid in the first reaction part.
Similarly, in some embodiments, the control device 70 further includes, after controlling the detection device 50 to perform a specific protein detection operation on the specific protein detection liquid in the second reaction portion: the cleaning device is controlled to clean the second reaction part and the pipetting needle so as to discharge the specific protein detection liquid in the second reaction part.
It should be appreciated that after the detection operation is completed, the pipetting needle and the corresponding reaction portion are cleaned by the cleaning device, so that the residual detection liquid can be prevented from being remained in the reaction device and the dispensing device for a long time, the reaction device and the dispensing device are corroded, and the service life of the sample analyzer 100 is prolonged.
Referring to fig. 5, in some embodiments, the operation modes include a second operation mode, the reaction portion includes a first reaction portion, a second reaction portion, and a third reaction portion, and the control device 70 includes, in controlling the functional modules of the sample analyzer 100 to execute corresponding actions according to the operation modes to implement a specific item detection process:
in a state in which the sample analyzer 100 is in the second operation mode, the control device 70 controls the dispensing device 20 to respectively fill the first reaction part, the second reaction part, and the third reaction part with the sample and/or the corresponding reagent to form a first erythrocyte osmotic fragility detection liquid in any one of the first reaction part and the second reaction part, a second erythrocyte osmotic fragility detection liquid in the other one, and a blood routine detection liquid in the third reaction part;
the control detecting means 50 performs a first detecting operation of the erythrocyte osmotic fragility of the first erythrocyte osmotic fragility detecting liquid in the first reaction part or the second reaction part, and performs a second detecting operation of the erythrocyte osmotic fragility of the second erythrocyte osmotic fragility detecting liquid in the first reaction part or the second reaction part, and performs a blood routine detecting operation of the blood routine detecting liquid in the third reaction part.
It should be appreciated that, when the sample analyzer 100 is in the second operation mode, the sample analyzer 100 is at least used for performing erythrocyte osmotic fragility detection and blood routine detection on the sample to be detected.
Specifically, in a state in which the sample analyzer 100 is in the first operation mode, the control device 70 controls the dispensing device 20 to fill the first reaction part, the second reaction part, and the third reaction part with the sample and/or the corresponding reagent, respectively, to form the first erythrocyte osmotic fragility detection liquid and the second erythrocyte osmotic fragility detection liquid in the first reaction part and the second reaction part, respectively, and to form the blood regular detection liquid in the third reaction part. Wherein the first reaction part, the second reaction part and the third reaction part are separately arranged. After the corresponding detection liquids are formed in the first reaction part, the second reaction part and the third reaction part, respectively, the control device 70 controls the detection device 50 to perform the first detection operation of the erythrocyte osmotic fragility and the second detection operation of the erythrocyte osmotic fragility on the first erythrocyte osmotic fragility detection liquid and the second erythrocyte osmotic fragility detection liquid in the first reaction part and the second reaction part, respectively, and to perform the blood routine detection on the blood routine detection liquid in the third reaction part.
It is understood that the red blood cell osmotic fragility test procedure includes at least a red blood cell osmotic fragility first test procedure and a red blood cell osmotic fragility first test procedure. Through carrying out the red blood cell infiltration friability detection operation of two parts respectively in first reaction portion and second reaction portion, greatly improved the detection efficiency of red blood cell infiltration friability detection operation, when realizing red blood cell infiltration friability detection and blood routine detection effectively, reduced detection cost, reduced detection work load to the required time of sample detection has been practiced thrift.
In some embodiments, the control device 70 is further configured to:
in a state in which the sample analyzer 100 is in the second operation mode, the dispensing device 20 is controlled to add the first osmotic fragility detection reagent to the second reaction part;
after the addition of the first osmotic fragility detection reagent is completed, controlling the dispensing device 20 to draw the sample into the sample container and add the sample into the first reaction part carrying the first osmotic fragility detection reagent so as to form a first erythrocyte osmotic fragility detection liquid in the first reaction part; and controlling the detecting means 50 to perform a first detecting operation of the erythrocyte osmotic fragility on the first erythrocyte osmotic fragility detecting liquid in the first reaction part;
In the process of performing the first detection operation of erythrocyte osmotic fragility, controlling the dispensing device 20 to add a sample to the third reaction part so as to form a blood routine detection liquid in the third reaction part, and controlling the detecting device 50 to perform a blood routine detection operation on the blood routine detection liquid in the third reaction part;
after the operation of adding the sample to the third reaction part by the dispensing device 20 is completed, controlling the dispensing device 20 to add the second osmotic fragility detection reagent and the sample to the first reaction part so as to form a second erythrocyte osmotic fragility detection liquid in the first reaction part; and controls the detecting means 50 to perform the second detecting operation of the erythrocyte osmotic fragility with respect to the second erythrocyte osmotic fragility detecting liquid in the first reaction part.
Fig. 5 is a timing diagram of another workflow of the sample analyzer 100 in the second operation mode according to the embodiment of the present application.
As shown in fig. 5, specifically, in the state where the sample analyzer 100 is in the second operation mode, the control device 70 first controls the dispensing device 20 to add the first osmotic fragility detection reagent to the second reaction part. After the addition of the first osmotic fragility detection reagent is completed, the control device 70 controls the dispensing device 20 to draw the sample into the sample container and to add the sample into the second reaction part carrying the first osmotic fragility detection reagent so as to form a first erythrocyte osmotic fragility detection liquid in the second reaction part, and then controls the detection device 50 to perform an erythrocyte osmotic fragility first detection operation on the first erythrocyte osmotic fragility detection liquid in the second reaction part;
In performing the first detection operation of erythrocyte osmotic fragility, the control device 70 controls the dispensing device 20 to add a sample to the third reaction part so that a blood routine detection liquid is formed in the third reaction part, and controls the detection device 50 to perform a blood routine detection operation on the blood routine detection liquid in the third reaction part. After the completion of the operation of adding the sample to the third reaction portion by the dispensing device 20, the control device 70 controls the dispensing device 20 to add the second osmotic fragility detection reagent and the sample to the first reaction portion so that the second erythrocyte osmotic fragility detection liquid is formed in the first reaction portion, and then controls the detection device 50 to perform the erythrocyte osmotic fragility second detection operation on the second erythrocyte osmotic fragility detection liquid in the first reaction portion.
In some embodiments, the control device 70 further includes, after controlling the detecting device 50 to perform the second detecting operation of the erythrocyte osmotic fragility of the second erythrocyte osmotic fragility detecting liquid in the first reaction part: the cleaning device is controlled to clean the first reaction part and the pipetting needle so as to discharge the second erythrocyte osmotic fragility detection liquid in the first reaction part.
Similarly, in some embodiments, the control device 70 further includes, after controlling the detection device 50 to perform the first detection operation of erythrocyte osmotic fragility on the specific protein detection liquid in the second reaction part: the cleaning device is controlled to clean the second reaction part and the pipetting needle so as to discharge the specific protein detection liquid in the second reaction part.
It should be appreciated that after the detection operation is completed, the pipetting needle and the corresponding reaction portion are cleaned by the cleaning device, so that the residual detection liquid can be prevented from being remained in the reaction device and the dispensing device for a long time, the reaction device and the dispensing device are corroded, and the service life of the sample analyzer 100 is prolonged.
In other embodiments, the operation modes include a second operation mode, the reaction part includes a first reaction part and a third reaction part, and the control device 70 includes, in controlling the functional module of the sample analyzer 100 to perform corresponding actions according to the operation mode to implement a specific item detection process:
in a state in which the sample analyzer 100 is in the second operation mode, the control device 70 controls the dispensing device 20 to fill the first reaction part and the third reaction part with the sample and/or the corresponding reagent, respectively, to form a erythrocyte osmotic fragility detection liquid in the first reaction part and a blood routine detection liquid in the third reaction part;
the control detecting device 50 detects the erythrocyte osmotic fragility of the erythrocyte osmotic fragility detecting liquid in the first reaction part and detects the blood routine of the blood routine detecting liquid in the third reaction part.
Referring to fig. 6, in some embodiments, the control device 70 is further configured to:
In a state in which the sample analyzer 100 is in the second operation mode, the dispensing device 20 is controlled to add the first osmotic fragility detection reagent to the first reaction part;
after the addition of the first osmotic fragility detection reagent is completed, controlling the dispensing device 20 to draw the sample into the sample container and add the sample into the first reaction part carrying the first osmotic fragility detection reagent so as to form a first erythrocyte osmotic fragility detection liquid in the first reaction part; and controlling the detecting means 50 to perform a first detecting operation of the erythrocyte osmotic fragility on the first erythrocyte osmotic fragility detecting liquid in the first reaction part;
in the process of performing the first detection operation of erythrocyte osmotic fragility, controlling the dispensing device 20 to add a sample to the third reaction part so as to form a blood routine detection liquid in the third reaction part, and controlling the detecting device 50 to perform a blood routine detection operation on the blood routine detection liquid in the third reaction part;
after the execution of the operation of adding the sample to the third reaction part is completed, controlling the dispensing device 20 to add the second osmotic fragility detection reagent and the sample to the first reaction part so as to form a second erythrocyte osmotic fragility detection liquid in the first reaction part; and controls the detecting means 50 to perform the second detecting operation of the erythrocyte osmotic fragility with respect to the second erythrocyte osmotic fragility detecting liquid in the first reaction part.
Fig. 6 is a timing diagram of another workflow of the sample analyzer 100 in the second operation mode according to the embodiment of the present application.
As shown in fig. 6, specifically, in another workflow sequence in which the sample analyzer 100 is in the second operation mode state, the control device 70 first controls the dispensing device 20 to add the first osmotic fragility detection reagent to the first reaction part. After the addition of the first osmotic fragility test agent is completed, the control device 70 controls the dispensing device 20 to draw the sample into the sample container and to add the sample to the first reaction part carrying the first osmotic fragility test agent so that the first erythrocyte osmotic fragility test liquid is formed in the first reaction part, and then controls the test device 50 to perform the erythrocyte osmotic fragility first test operation on the first erythrocyte osmotic fragility test liquid in the first reaction part.
In performing the first detection operation of erythrocyte osmotic fragility, the control device 70 controls the dispensing device 20 to add a sample to the third reaction part so that a blood routine detection liquid is formed in the third reaction part, and controls the detection device 50 to perform a blood routine detection operation on the blood routine detection liquid in the third reaction part. After the execution of the operation of adding the sample to the third reaction part is completed, the control device 70 controls the dispensing device 20 to add the second osmotic fragility detection reagent and the sample to the first reaction part so that the second erythrocyte osmotic fragility detection liquid is formed in the first reaction part, and then controls the detection device 50 to execute the erythrocyte osmotic fragility second detection operation on the second erythrocyte osmotic fragility detection liquid in the first reaction part.
The control device 70 controls the dispensing device 20 and the detecting device 50 to sequentially complete the steps, so that the dispensing device and the detecting device 50 work in a highly matched mode, the detecting efficiency of erythrocyte osmotic fragility detection and blood routine detection on a sample is improved, the detecting cost is reduced, the detecting workload is reduced, and the time required by sample detection is saved.
In some embodiments, the control device 70 further includes, after controlling the detecting device 50 to perform the first detecting operation of the erythrocyte osmotic fragility detecting liquid in the first reaction part: the cleaning device is controlled to clean the first reaction part so as to discharge the first erythrocyte osmotic fragility detection liquid in the first reaction part. Thereby avoiding the pollution of the first erythrocyte osmotic fragility detection liquid to the second erythrocyte osmotic fragility detection operation.
Referring to fig. 7, in an embodiment, the control device 70 is further configured to:
in a state in which the sample analyzer 100 is in the second operation mode, controlling the dispensing device 20 to aspirate a sample into the sample container and to add the sample to the first reaction portion carrying the first osmotic fragility detection reagent so as to form a first erythrocyte osmotic fragility detection liquid in the first reaction portion; and controlling the detecting means 50 to perform a first detecting operation of the erythrocyte osmotic fragility on the first erythrocyte osmotic fragility detecting liquid in the first reaction part;
In the process of performing the first detection operation of erythrocyte osmotic fragility, controlling the dispensing device 20 to add a sample to the third reaction part so as to form a blood routine detection liquid in the third reaction part, and controlling the detecting device 50 to perform a blood routine detection operation on the blood routine detection liquid in the third reaction part;
after the execution of the first detection operation of erythrocyte osmotic fragility is completed, controlling the dispensing device 20 to add a sample to the first reaction part carrying the second osmotic fragility detection reagent so as to form a second erythrocyte osmotic fragility detection liquid in the first reaction part; and controls the detecting means 50 to perform the second detecting operation of the erythrocyte osmotic fragility with respect to the second erythrocyte osmotic fragility detecting liquid in the first reaction part.
Fig. 7 is a timing diagram of still another workflow of the sample analyzer 100 in the second operation mode according to the embodiment of the present application.
As shown in fig. 7, specifically, in still another operation flow sequence in which the sample analyzer 100 is in the second operation mode, the control device 70 first controls the dispensing device 20 to aspirate the sample into the sample container and to add the sample to the first reaction portion carrying the first osmotic fragility detection reagent so that the first erythrocyte osmotic fragility detection liquid is formed in the first reaction portion, and then controls the detection device 50 to perform the erythrocyte osmotic fragility first detection operation on the first erythrocyte osmotic fragility detection liquid in the first reaction portion.
In performing the first detection operation of erythrocyte osmotic fragility, the control device 70 controls the dispensing device 20 to add a sample to the third reaction part so that a blood routine detection liquid is formed in the third reaction part, and controls the detection device 50 to perform a blood routine detection operation on the blood routine detection liquid in the third reaction part. After the completion of the first erythrocyte osmotic fragility test operation, the control device 70 controls the dispensing device 20 to add a sample to the first reaction part carrying the second osmotic fragility test reagent so that the second erythrocyte osmotic fragility test liquid is formed in the first reaction part, and then controls the test device 50 to perform the second erythrocyte osmotic fragility test operation on the second erythrocyte osmotic fragility test liquid in the first reaction part.
In some embodiments, the control device 70 further includes, after controlling the detecting device 50 to perform the first detecting operation of the erythrocyte osmotic fragility detecting liquid in the first reaction part: the cleaning device is controlled to clean the first reaction part so as to discharge the first erythrocyte osmotic fragility detection liquid in the first reaction part. Thereby avoiding the pollution of the first erythrocyte osmotic fragility detection liquid to the second erythrocyte osmotic fragility detection operation.
In some embodiments, the reagents include a specific protein reagent and a red blood cell permeable fragile reagent, and the sample analyzer 100 further includes a cryopreservation device for cryopreserving at least one of the specific protein reagent and the red blood cell permeable fragile reagent.
Referring to fig. 8, the present application further provides a control method applied to the sample analyzer 100 provided in any embodiment of the present application, where the control method provided in the present application specifically includes:
step S101: determining an operating mode of the sample analyzer;
step S102: and controlling a functional module of the sample analyzer to execute corresponding actions according to the working mode so as to realize specific item detection, wherein the functional module at least comprises a dispensing device and a detection device, and the specific detection item comprises at least one of specific protein detection and blood routine detection and erythrocyte osmotic fragility detection.
Specifically, the sample analyzer comprises a dispensing device, a reaction device and a detection device; the dispensing device is used for transferring samples and/or reagents; the reaction device is provided with at least two reaction parts, the reaction parts are used for providing reaction places for samples and/or reagents transferred by the dispensing device, so that the samples and the reagents in the corresponding reaction parts are mixed to form a sample liquid to be tested, and the sample liquid to be tested comprises at least one of a specific protein detection liquid and a blood conventional detection liquid and a red blood cell permeation fragility detection liquid; the detection device is used for carrying out specific protein detection on the specific protein detection liquid in the reaction part and/or carrying out blood routine detection on the blood routine detection liquid, and carrying out red blood cell permeation fragility detection on the red blood cell permeation fragility detection liquid in the reaction part;
It should be noted that, for convenience and brevity of description, specific working procedures of the control method of the sample analyzer described above may refer to corresponding working procedures of the sample analyzer, and will not be described herein.
It is to be understood that the terminology used in the description of the present application is for the purpose of describing particular embodiments only and is not intended to be limiting of the application. As used in this specification and the appended claims, the singular forms "a," "an," and "the" are intended to include the plural forms as well, unless the context clearly indicates otherwise.
It should also be understood that the term "and/or" as used in this specification and the appended claims refers to any and all possible combinations of one or more of the associated listed items, and includes such combinations. It should be noted that, in this document, the terms "comprises," "comprising," or any other variation thereof, are intended to cover a non-exclusive inclusion, such that a process, method, article, or system that comprises a list of elements does not include only those elements but may include other elements not expressly listed or inherent to such process, method, article, or system. Without further limitation, an element defined by the phrase "comprising one … …" does not exclude the presence of other like elements in a process, method, article, or system that comprises the element.
The foregoing embodiment numbers of the present application are merely for describing, and do not represent advantages or disadvantages of the embodiments. While the invention has been described with reference to certain preferred embodiments, it will be understood by those skilled in the art that various changes and substitutions of equivalents may be made and equivalents will be apparent to those skilled in the art without departing from the scope of the invention. Therefore, the protection scope of the present application shall be subject to the protection scope of the claims.
Claims (12)
1. A sample analyzer, comprising:
a dispensing device for transferring a sample and/or a reagent;
a reaction device having at least two reaction parts for providing reaction sites for the samples and/or the reagents transferred by the dispensing device, so that the samples and the corresponding reagents in the corresponding reaction parts are mixed to form a sample liquid to be tested, wherein the sample liquid to be tested comprises at least one of a specific protein detection liquid and a blood routine detection liquid, and a red blood cell permeation fragility detection liquid;
a detection device for detecting a specific protein in the specific protein detection liquid and/or detecting a blood routine in the blood routine detection liquid in the reaction part, and detecting a red blood cell osmotic fragility in the red blood cell osmotic fragility detection liquid in the reaction part;
Control means, in communication with said dispensing means and said detecting means, for:
determining a working mode of the sample analyzer, and controlling a functional module of the sample analyzer to execute corresponding actions according to the working mode so as to realize specific item detection, wherein the functional module at least comprises the dispensing device and the detection device, and the specific detection item comprises at least one of specific protein detection and blood routine detection, and erythrocyte osmotic fragility detection.
2. The sample analyzer of claim 1, wherein the operation modes include a first operation mode, the reaction portion includes a first reaction portion, a second reaction portion, and a third reaction portion, and the control device includes, in controlling the functional module of the sample analyzer to perform corresponding actions to achieve a specific item detection process according to the operation modes:
in a state that the sample analyzer is in the first working mode, the control device controls the dispensing device to respectively fill the first reaction part, the second reaction part and the third reaction part with the sample and/or corresponding reagent so as to form a red blood cell osmotic fragility detection liquid in the first reaction part, a specific protein detection liquid in the second reaction part and a blood routine detection liquid in the third reaction part;
Controlling the detection device to perform erythrocyte osmotic fragility detection on the erythrocyte osmotic fragility detection liquid of the first reaction part, performing specific protein detection on the specific protein detection liquid of the second reaction part, and performing blood routine detection on the blood routine detection liquid in the third reaction part.
3. The sample analyzer of claim 2, wherein the control device is further configured to:
controlling the dispensing device to add a first osmotic fragility detection reagent to the first reaction part in a state that the sample analyzer is in the first working mode;
after the addition of the first osmotic fragility detection reagent is completed, controlling the dispensing device to suck the sample into a sample container, and adding the sample into the first reaction part carrying the first osmotic fragility detection reagent so as to form a first erythrocyte osmotic fragility detection liquid in the first reaction part; and controlling the detection device to perform a first detection operation of erythrocyte osmotic fragility on a first erythrocyte osmotic fragility detection liquid in the first reaction part;
in the process of executing the first detection operation of the erythrocyte osmotic fragility, controlling the dispensing device to add the sample to the third reaction part so as to form blood routine detection liquid in the third reaction part, and controlling the detecting device to execute the blood routine detection operation on the blood routine detection liquid in the third reaction part;
After the addition of the sample to the third reaction portion is completed, controlling the dispensing device to add the sample to the second reaction portion carrying a first specific protein detection reagent;
after the operation of adding the sample to the second reaction part is completed and after the first detection operation of erythrocyte fragility is completed, controlling the dispensing device to add the sample to the first reaction part bearing a second osmotic fragility detection reagent so as to form a second erythrocyte osmotic fragility detection liquid in the first reaction part; and controlling the detection device to execute a second detection operation of the erythrocyte osmotic fragility on a second erythrocyte osmotic fragility detection liquid in the first reaction part;
controlling the dispensing device to add a second specific protein detection reagent to the second reaction part carrying the mixed liquid of the first specific protein detection reagent and the sample during the execution of the second detection operation of erythrocyte osmotic fragility, so that a specific protein detection liquid is formed in the second reaction part; and controlling the detection device to detect the specific protein in the specific protein detection liquid in the second reaction part.
4. A sample analyzer according to claim 3, wherein the control means, in controlling the dispensing means to add a first osmotic fragility detection reagent to the first reaction part, comprises:
Controlling the dispensing device to add a first amount of the first osmotic fragility-detecting reagent to the first reaction part to perform a first washing operation on the first reaction part;
after the first washing operation is completed, the dispensing device is controlled to add a second amount of the first osmotic fragility detection reagent to the first reaction part.
5. The sample analyzer of claim 4, wherein the second amount is greater than the first amount.
6. The sample analyzer of claim 2, wherein the control device is further configured to:
controlling the dispensing device to suck the sample into a sample container in a state that the sample analyzer is in the first working mode, and controlling the dispensing device to add the sample into the first reaction part carrying the first osmotic fragility detection reagent after the sample sucking operation is completed so as to form a first erythrocyte osmotic fragility detection liquid in the first reaction part; and controlling the detection device to perform a first detection operation of erythrocyte osmotic fragility on a first erythrocyte osmotic fragility detection liquid in the first reaction part;
in the process of executing the first detection operation of the erythrocyte osmotic fragility, controlling the dispensing device to add the sample to the third reaction part so as to form blood routine detection liquid in the third reaction part, and controlling the detecting device to execute the blood routine detection operation on the blood routine detection liquid in the third reaction part;
After the execution of the operation of adding the sample to the third reaction part is completed, controlling the dispensing device to add the sample to the second reaction part carrying a first specific protein detection reagent;
after the operation of adding the sample to the second reaction part is completed, controlling the dispensing device to add the sample to the first reaction part carrying a second osmotic fragility detection reagent so as to form a second erythrocyte osmotic fragility detection liquid in the first reaction part; and controlling the detection device to execute a second detection operation of the erythrocyte osmotic fragility on a second erythrocyte osmotic fragility detection liquid in the first reaction part;
controlling the dispensing device to add a second specific protein detection reagent to the second reaction part carrying the mixed liquid of the first specific protein detection reagent and the sample during the execution of the second detection operation of erythrocyte osmotic fragility, so that a specific protein detection liquid is formed in the second reaction part; and controlling the detection device to detect the specific protein in the specific protein detection liquid in the second reaction part.
7. The sample analyzer of claim 1, wherein the operation modes include a second operation mode, the reaction portion includes a first reaction portion, a second reaction portion, and a third reaction portion, and the control device includes, in controlling the functional module of the sample analyzer to perform corresponding actions to achieve a specific item detection process according to the operation modes:
In a state in which the sample analyzer is in the second operation mode, the control device controls the dispensing device to fill the first reaction part, the second reaction part, and the third reaction part with the sample and/or the corresponding reagent, respectively, so as to form a first erythrocyte osmotic fragility detection liquid in any one of the first reaction part and the second reaction part, form a second erythrocyte osmotic fragility detection liquid in the other one, and form a blood routine detection liquid in the third reaction part;
controlling the detection device to perform a first detection operation of the erythrocyte osmotic fragility of a first erythrocyte osmotic fragility detection liquid in the first reaction part or the second reaction part, perform a second detection operation of the erythrocyte osmotic fragility of a second erythrocyte osmotic fragility detection liquid in the first reaction part or the second reaction part, and perform a blood routine detection of the blood routine detection liquid in the third reaction part.
8. The sample analyzer of claim 7, wherein the control device is further configured to:
controlling the dispensing device to add a first osmotic fragility detection reagent to the second reaction part in a state that the sample analyzer is in the second working mode;
After the addition of the first osmotic fragility detection reagent is completed, controlling the dispensing device to suck the sample into a sample container, and adding the sample into the second reaction part carrying the first osmotic fragility detection reagent so as to form a first erythrocyte osmotic fragility detection liquid in the second reaction part; and controlling the detection device to perform a first detection operation of the erythrocyte osmotic fragility on the first erythrocyte osmotic fragility detection liquid in the second reaction part;
in the process of executing the first detection operation of the erythrocyte osmotic fragility, controlling the dispensing device to add the sample to the third reaction part so as to form blood routine detection liquid in the third reaction part, and controlling the detecting device to execute the blood routine detection operation on the blood routine detection liquid in the third reaction part;
after the operation of adding the sample to the third reaction part by the dispensing device is completed, controlling the dispensing device to add a second osmotic fragility detection reagent and the sample to the first reaction part so as to form a second erythrocyte osmotic fragility detection liquid in the first reaction part; and controlling the detecting device to execute a second detecting operation of the erythrocyte osmotic fragility on a second erythrocyte osmotic fragility detecting liquid in the first reaction part.
9. The sample analyzer of claim 1, wherein the operation modes include a second operation mode, the reaction portion includes a first reaction portion and a third reaction portion, and the control device includes, in controlling the functional module of the sample analyzer to perform corresponding actions according to the operation modes to implement a specific item detection process:
in a state that the sample analyzer is in the second working mode, the control device controls the dispensing device to respectively fill the first reaction part and the third reaction part with the sample and/or the corresponding reagent so as to form a red blood cell osmotic fragility detection liquid in the first reaction part and a blood routine detection liquid in the third reaction part;
controlling the detection device to perform erythrocyte osmotic fragility detection on the erythrocyte osmotic fragility detection liquid of the first reaction part, and performing blood routine detection on the blood routine detection liquid in the third reaction part.
10. The sample analyzer of claim 9, wherein the control device is further configured to:
controlling the dispensing device to add a first osmotic fragility detection reagent to the first reaction part in a state that the sample analyzer is in the second working mode;
After the addition of the first osmotic fragility detection reagent is completed, controlling the dispensing device to suck the sample into a sample container, and adding the sample into the first reaction part carrying the first osmotic fragility detection reagent so as to form a first erythrocyte osmotic fragility detection liquid in the first reaction part; and controlling the detection device to perform a first detection operation of erythrocyte osmotic fragility on a first erythrocyte osmotic fragility detection liquid in the first reaction part;
in the process of executing the first detection operation of the erythrocyte osmotic fragility, controlling the dispensing device to add the sample to the third reaction part so as to form blood routine detection liquid in the third reaction part, and controlling the detecting device to execute the blood routine detection operation on the blood routine detection liquid in the third reaction part;
after the sample is added to the third reaction part, controlling the dispensing device to add a second osmotic fragility detection reagent and the sample to the first reaction part so as to form a second erythrocyte osmotic fragility detection liquid in the first reaction part; and controlling the detection device to execute a second detection operation of the erythrocyte osmotic fragility on a second erythrocyte osmotic fragility detection liquid in the first reaction part;
Or in a state that the sample analyzer is in the second working mode, controlling the dispensing device to suck the sample to a sample container, and adding the sample to the first reaction part loaded with the first osmotic fragility detection reagent so as to form a first erythrocyte osmotic fragility detection liquid in the first reaction part; and controlling the detection device to perform a first detection operation of erythrocyte osmotic fragility on a first erythrocyte osmotic fragility detection liquid in the first reaction part;
in the process of executing the first detection operation of the erythrocyte osmotic fragility, controlling the dispensing device to add the sample to the third reaction part so as to form blood routine detection liquid in the third reaction part, and controlling the detecting device to execute the blood routine detection operation on the blood routine detection liquid in the third reaction part;
after the execution of the first detection operation of the erythrocyte osmotic fragility is finished, controlling the dispensing device to add the sample to the first reaction part bearing the second osmotic fragility detection reagent so as to form a second erythrocyte osmotic fragility detection liquid in the first reaction part; and controlling the detecting device to execute a second detecting operation of the erythrocyte osmotic fragility on a second erythrocyte osmotic fragility detecting liquid in the first reaction part.
11. The sample analyzer of any one of claims 1-10, wherein the reagent comprises a specific protein reagent and a red blood cell permeable fragile reagent, the sample analyzer further comprising a cryopreservation device for cryopreserving at least one of the specific protein reagent and the red blood cell permeable fragile reagent.
12. A control method applied to a sample analyzer, which is characterized in that the sample analyzer comprises a dispensing device, a reaction device and a detection device; the dispensing device is used for transferring samples and/or reagents; a reaction device having at least two reaction parts for providing reaction sites for the samples and/or the reagents transferred by the dispensing device, so that the samples and reagents in the corresponding reaction parts are mixed to form a sample liquid to be tested, wherein the sample liquid to be tested comprises at least one of a specific protein detection liquid and a blood routine detection liquid, and a red blood cell permeation fragility detection liquid; the detection device is used for carrying out specific protein detection on the specific protein detection liquid in the reaction part and/or carrying out blood routine detection on the blood routine detection liquid, and carrying out red blood cell osmotic fragility detection on the red blood cell osmotic fragility detection liquid in the reaction part;
The method comprises the following steps:
determining a working mode of the sample analyzer, and controlling a functional module of the sample analyzer to execute corresponding actions according to the working mode so as to realize specific item detection, wherein the functional module at least comprises the dispensing device and the detection device, and the specific detection item comprises at least one of specific protein detection and blood routine detection, and erythrocyte osmotic fragility detection.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202210763507.7A CN117368500A (en) | 2022-06-30 | 2022-06-30 | Sample analyzer and control method |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202210763507.7A CN117368500A (en) | 2022-06-30 | 2022-06-30 | Sample analyzer and control method |
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| Publication Number | Publication Date |
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| CN117368500A true CN117368500A (en) | 2024-01-09 |
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| CN202210763507.7A Pending CN117368500A (en) | 2022-06-30 | 2022-06-30 | Sample analyzer and control method |
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| Country | Link |
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| CN (1) | CN117368500A (en) |
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