CN117338654A - A kind of samphire mosquito repellent and antipruritic gel and preparation method thereof - Google Patents

Abstract

The invention discloses a Salicornia europaea mosquito-repellent itching-relieving gel and a preparation method thereof, wherein the Salicornia europaea mosquito-repellent itching-relieving gel comprises, by weight, 20-25 parts of Salicornia europaea juice, 0.3-0.5 part of wormwood extract, 0.5-0.6 part of peppermint oil, 0.6-0.7 part of carbomer, 4-5 parts of propylene glycol, 4-5 parts of glycerin, 1-2 parts of pH regulator, 1-2 parts of penetration enhancer, 0.1 part of preservative and 18-20 parts of deionized water. The Salicornia bigelovii mosquito-repellent itching-relieving gel has the characteristics of better mosquito-repellent, itching-relieving, detumescence and the like, has the advantages of natural and harmless raw materials, small skin irritation, quick drug absorption, lasting drug effect and the like, and is friendly to sensitive skin people. The product not only improves the high-value utilization of the medicinal and edible halophyte resource Salicornia bigelovii, but also widens the variety of mosquito-repellent itching-relieving products in the market, and has wide market prospect.

Description

A kind of samphire mosquito repellent and antipruritic gel and preparation method thereof

Technical field

The invention belongs to the field of daily chemical products, and specifically relates to a samphire mosquito repellent and antipruritic gel and a preparation method thereof.

Background technique

Salicornia belongs to the genus Salicornia of the family Chenopodiaceae, and is commonly known as sea asparagus or sea beans. This genus is distributed all over the world and has about fifty species. It grows in saline-alkali soil and can be irrigated directly with salt water. Samphire has a strong vitality and reproductive capacity. As a plant with dual uses as food and medicine, samphire is recorded in "Chinese Materia Medica". It has good effects on blood pressure, headaches, liver yin deficiency, etc. Salicornia contains a variety of active ingredients, such as flavonoids, alkaloids, steroids, etc. As a traditional Chinese herbal medicine, it has been used to calm the liver, lower lipids and blood pressure, activate blood circulation and fight inflammation, relieve constipation and other problems. Salicornia also It has the effect of losing weight. Salicornia is also used to treat skin conditions such as itchy skin and rashes. In recent years, studies have shown that Salicornia can inhibit apoptosis and protect human skin fibroblasts from oxidation by tert-butyl hydroperoxide. At the same time, samphire extract can reduce the synthesis of melanin in the human body, which can inhibit the activity of tyrosinase. Therefore, Salicornia has great potential in both food applications and medicinal applications.

A gel is a viscous liquid or semi-solid preparation that is combined with a suitable matrix and has certain gel properties. Gel ingredients include base, excipients and main drug. Gels have many advantages. Unlike common external preparations such as ointments, creams, and liniments, gels do not apply unevenly when using ointments and creams, causing contamination of clothes; Agents and liniments, highly volatile and easy to spread. Gels generally have a fine and stable texture, moderate fluidity, can be easily applied to the skin, have strong adhesion, are quickly absorbed by the human body, do not irritate the skin and mucous membranes, and the preparation process is simple and convenient. In addition, a wide range of drugs can be combined with the gel matrix, including crystalline or powdered chemical drugs, Chinese herbal medicine powders or extracts, etc.

However, there have been no reports on research on samphire-related mosquito repellent products.

Contents of the invention

In view of the shortcomings of the existing technology, the present invention provides a samphire mosquito repellent and anti-itching gel and a preparation method thereof, which have the advantages of natural and harmless raw materials, little irritation to the skin, fast drug absorption and long-lasting drug effect.

The present invention is achieved through the following technical solutions:

A kind of samphire mosquito repellent and anti-pruritic gel contains, by weight, 20 to 25 parts of samphire juice, 0.3 to 0.5 parts of mugwort extract, 0.5 to 0.6 parts of peppermint oil, and 0.6 to 0.7 parts of carbomer. 4 to 5 parts of propylene glycol, 4 to 5 parts of glycerin, 1 to 2 parts of pH regulator, 1 to 2 parts of penetration enhancer, 0.1 part of preservative, 18 to 20 parts of deionized water; among them, samphire juice, mugwort Extract and peppermint oil are the raw materials of the gel; carbomer is the gel base; propylene glycol and glycerin are the moisturizers.

Preferably, the pH adjuster is triethanolamine, the penetration enhancer is azone, and the preservative is sodium benzoate.

A preparation method of Samphire mosquito repellent and anti-itch gel, including the following steps:

Step 1) Preprocessing of raw materials: collect fresh seaweed, wash, dry and set aside; take the above-ground part of fresh mugwort, clean it, dry it at room temperature, crush it and set aside; take fresh mint, clean, dry and cut it into short sections for later use;

Step 2) Preparation of samphire juice: take the drained fresh samphire juice, crush it and squeeze the juice, filter the juice to remove the residue, add vitamin C, and prepare samphire juice for later use;

Step 3) Preparation of mugwort extract: Prepared by supercritical CO ₂ fluid extraction technology. Take the mugwort treated in step 1) and fill it evenly into the extraction reaction kettle. Use CO ₂ to repeatedly flush the air in the pipeline; liquefy it. The final CO ₂ is pumped into the mixer through a high-frequency plunger pump, the parameters are adjusted, and the mugwort extract is prepared for later use;

Step 4) Preparation of peppermint oil: Prepare by cooking method, take mint raw materials, extract according to the ratio of material to liquid ratio 1:8, keep it in a slightly boiling state for more than 4 hours, and dry and filter the prepared peppermint oil with anhydrous sodium sulfate. spare;

Step 5) Preparation of samphire gel: Weigh carbomer, propylene glycol, glycerin, triethanolamine, azone, sodium benzoate and deionized water in parts by weight; sprinkle the carbomer evenly on the surface of the deionized water , leave to swell for 24 hours; add corresponding amounts of propylene glycol, glycerin, azone, sodium benzoate, and the samphire juice, mugwort extract and mentha oil prepared in steps 1) to 3) into the gel matrix in sequence , add the reagent each time and stir it evenly with a mixer before adding the next reagent. Finally, slowly add distilled water and stir evenly to prepare the samphire gel;

Step 6) Adjusting the pH value of the samphire gel: Use triethanolamine to adjust the pH value of the samphire gel prepared in step 5), stir evenly, and adjust the pH value to 6-8;

Step 7) Sterilize the samphire gel treated in step 6) with hydroperoxide gel to obtain a green transparent gel. After filling, the finished product of samphire repellent and anti-itch gel is obtained .

Preferably, the amount of vitamin C added in step 2) is 0.5g/kg samphire.

Preferably, the settings of the control parameters in step 3) are as follows: control the CO ₂ flow rate to 25L/h, set the reactor separation pressure I to 10±0.2MPa, set the temperature to 55°C, and set the separation pressure II to 7±0.2 MPa, the temperature is 55℃.

Preferably, the amount of distilled water added in step 5) is: dilute to 50 parts by weight.

Preferably, the weight of the finished samphire mosquito repellent and anti-itch gel in step 7) is 50g.

The beneficial effects of the present invention are as follows:

The present invention is based on the medicinal properties of Salicornia, mugwort and mint, and flexibly uses the principle of synergy of active ingredients to explore the formula and production process. It mainly uses medicinal and food resources - the antibacterial, anti-inflammatory, detumescent, antipruritic and other characteristics of samphire, the aroma of mugwort, which can stimulate the orifices, activate collaterals and tendons, and stimulate local blood circulation, combined with the detoxifying, rash-relieving, cooling and soothing effects of mint. Efficacy of Itching Prepare mosquito repellent and anti-itch gel. The samphire mosquito repellent and anti-pruritic gel of the present invention has the characteristics of better mosquito repellent, anti-itching, and swelling reduction, and has the advantages of natural and harmless raw materials, little irritation to the skin, fast drug absorption, and long-lasting drug effect. And friendly to people with sensitive skin. This product not only improves the high-value utilization of samphire, a homologous medicinal and edible halophyte resource, but also broadens the types of mosquito repellent and anti-pruritic products on the market, and has broad market prospects.

Description of drawings

Figure 1 is the rutin standard curve in Example 2;

Figure 2 is a chromatogram of the blank receiving solution in the in vitro transdermal experiment in Example 3;

Figure 3 is a chromatogram of the receiving solution at the 2nd hour of the in vitro transdermal experiment in Example 3;

Figure 4 is a chromatogram of rutin in the skin after 12 hours of the in vitro transdermal experiment in Example 3.

Detailed ways

The present invention will be further described in detail below in conjunction with the accompanying drawings and specific embodiments.

Unless otherwise specified, the technical means used in the following examples are conventional means well known to those skilled in the art. Experimental methods without specifying specific conditions are all conventional methods in the art.

The materials, reagents, etc. used in the following examples can all be obtained from commercial sources unless otherwise specified.

The experimental animals used in the following examples were Kunming mice, purchased from Beijing Vitong Lever Laboratory Animal Technology Co., Ltd.

Example 1

A kind of samphire mosquito repellent and anti-pruritic gel, the formula is as follows: 20-25g samphire juice, 0.3-0.5g mugwort extract, 0.5-0.6g menthae oil, 0.6-0.7g carbomer, propylene glycol 4 ~5g, glycerin 4~5g, triethanolamine 1~2g, azone 1~2g, sodium benzoate 0.1g, deionized water 18~20g.

A method for preparing a samphire mosquito repellent and antipruritic gel. The specific steps are as follows:

(1) Raw material pretreatment: Collect fresh seaweed, wash the sediment and dry the water, and set aside; clean the upper part of the fresh mugwort grass to remove sediment and other impurities, dry it at room temperature, dry it in a vacuum drying oven, crush it and set aside ; Fresh mint, after removing sediment and other impurities, dry it in a vacuum drying oven, and cut it into 5mm short sections for later use.

(2) Preparation of the active substance of Salicornia samphire (Samphire samphire juice): Take the drained fresh samphire samphire and crush it with a wall breaker to squeeze the juice. Filter the juice with filter paper to remove the residue and add a certain amount of vitamin C for use. For color protection, the added amount is 0.5g/kg samphire to ensure the green color and light transmittance of the product.

(3) Preparation of wormwood extract (wormwood essential oil): Use supercritical CO ₂ fluid extraction technology to prepare wormwood essential oil, take an appropriate amount of wormwood and evenly fill it in the extraction reaction kettle, and use CO ₂ to repeatedly flush to remove the air in the pipeline . Pump the liquefied _CO2 into the mixer through a high-frequency plunger pump, control the _CO2 flow rate to 25L/h, set the reactor separation pressure I to 10±0.2MPa, set the temperature to 55°C, and set the separation pressure II to 7± 0.2MPa, the temperature is 55℃, prepare mugwort essential oil for later use.

(4) Preparation of peppermint oil: Use the cooking method to prepare peppermint oil, take an appropriate amount of peppermint raw materials, extract according to the ratio of material to liquid ratio 1:8, keep it in a slightly boiling state for more than 4 hours, and use anhydrous sodium sulfate to prepare the peppermint oil. Dry, filter and set aside.

(5) Salicornia gel production process: Sprinkle carbomer evenly on the surface of deionized water and let it stand for 24 hours to swell. Add corresponding amounts of propylene glycol, glycerin, azone, sodium benzoate, samphire active substances, mugwort essential oil and mentha oil into the gel matrix in turn. Stir evenly with a mixer each time you add the reagent before adding the next one. reagent, and finally slowly add distilled water to 50g, stir evenly, and prepare a samphire gel.

(6) Adjusting the pH value of the samphire gel: Use triethanolamine to adjust the pH value of the samphire gel, stir evenly, and adjust the pH value to 6-8.

(7) The samphire gel prepared above is sterilized by hydroperoxide gel to obtain an emerald green transparent gel. After filling, the finished samphire repellent and anti-itch gel product is obtained.

The gel formula and preparation method of this embodiment were determined through relevant experiments in Example 2.

Example 2 Orthogonal selection of samphire mosquito repellent and antipruritic gel matrix and verification

1. Screening of gel matrix

Select commonly used gel matrix sodium alginate, carbomer 940, polyvinyl alcohol, and sodium carboxymethyl cellulose as the gel matrix screening objects. Take 1g of each gel matrix and add 40 mL of distilled water to fully swell evenly, and let it stand for 24 hours to obtain the result. Different gel matrices. The prepared gel matrix was evaluated, and a comprehensive evaluation was conducted based on the main criteria of the sample's molding, color, taste, viscosity, uniformity, spreadability, and centrifugal stability at 3000 r·min ^-1 . The results are shown in Table 1.

Table 1 Comprehensive evaluation of gel matrix

As can be seen from Table 1, the four gel matrices all have good stability. The color of sodium alginate is relatively turbid, polyvinyl alcohol is difficult to dissolve, and carbomer 940 and sodium carboxymethylcellulose are better formed than the other two matrices. , colorless and odorless, with moderate viscosity, but the uniformity of sodium carboxymethyl cellulose is slightly worse than that of carbomer 940. Based on the comparison results between the four, it was determined to select carbomer 940 as the base of the samphire mosquito repellent and anti-itch gel.

2. Prescription composition and preparation method of gel

Prescription composition: Salicornia active ingredients, mugwort essential oil, peppermint oil, mulberry leaf extract, carbomer 940, propylene glycol, glycerin, triethanolamine, azone, sodium benzoate, distilled water.

Preparation method: 24 hours before preparing the gel, take Carbomer 940 according to the prescription and place it in distilled water to fully swell. Add propylene glycol and glycerin to the swollen gel matrix to adjust its moisturizing degree, and add an appropriate amount of azone to improve its permeability. Add the raw materials of samphire actives, mugwort essential oil, and mentha oil, then use triethanolamine to adjust the pH of the gel to a pH value between 6-8, and finally add a small amount of preservative sodium benzoate. Add distilled water to a fixed mass of the gel, and grind it evenly to obtain a samphire repellent and anti-itch gel.

3. Single factor investigation experimental design of gel matrix

After preliminary experiments, the fixed mass of the samphire mosquito repellent and anti-itch gel is set to 50g, in which the fixed amount of samphire active substance as the raw material of the gel is 40%, and the fixed amount of moxa essential oil and peppermint essential oil as auxiliary agents. The addition amounts are 0.6% and 0.6% respectively, the fixed addition amount of glycerol as a moisturizer is 8%, the fixed addition amount of azone as a penetration enhancer is 1%, the fixed addition amount of sodium benzoate as a preservative is 0.2%, and Screen and investigate the dosage of carbomer 940, propylene glycol, and triethanolamine in the blank gel.

4. Comprehensive evaluation method of gel matrix

There are multiple measurement indicators for evaluating the gel matrix. In this example, a comprehensive scoring method is used to analyze the appearance, viscosity, pH, uniformity, and greasiness of the gel matrix. The following are the five indicators. Scoring standard, the comprehensive score is the sum of the five indicator scores (total score 60 points).

Appearance: color is translucent (11-15 points); color is slightly turbid (6-10 points); color is turbid and opaque (0-5 points).

Viscosity: Moderate viscosity, it flows slowly after tilting (11-15 minutes); overall slightly thin or thick, but flows too fast or too slow after tilting (6-10 minutes); too thin or too thick, flows too fast or Not flowing (0 to 5 points).

Uniformity: There are no obvious particles, and the application is relatively delicate (11 to 15 points); there are some particles after spreading (6 to 10 points); it is not easy to spread, there are obvious particles or it is too thin (0 to 5 points).

Degree of greasiness: not obviously greasy (11 to 15 points); greasy or dry (6 to 10 points); too oily or too thin (0 to 5 points).

pH: pH is within the range of 6-8 (+), pH is outside the range of 6-8 (-).

(1) Screening of the amount of carbomer 940 added

Under the condition that the raw materials and other auxiliary materials are fixed, since Carbopol 940 will have an impact on the viscosity of the gel, as shown in Table 2, the addition amount of Carbopol 940 is 0.7%, 1.0%, etc. %, 1.3%, 1.6%, and 1.9% are variables, and the optimal addition amount is screened out.

Table 2 Carbomer 940 addition amount design table

The five samples in Table 2 were examined from the five indicators of appearance, viscosity, uniformity, greasiness, and pH. The comprehensive scores are shown in Table 3.

Table 3 Comprehensive scoring results of carbomer 940 addition amount

It can be seen from Table 3 that when the addition amount of carbomer 940 is between 1.0% and 1.3%, the gel produced has a good appearance, high viscosity, good uniformity, no obvious greasiness, and the pH is within the acceptable range of the human body. Inside.

(2) Screening of propylene glycol dosage

Under the condition that the raw materials and other auxiliary materials are fixed, since propylene glycol will have an impact on the greasiness and uniformity of the gel, as shown in Table 4, the addition amount of propylene glycol is 3%, 6%, 9%, 12%, and 15% are variables, and the optimal addition amount is screened out.

Table 4 Propylene glycol addition amount design table

The five samples in Table 4 were examined from the five indicators of appearance, viscosity, uniformity, greasiness, and pH. The comprehensive scores are shown in Table 5.

Table 5 Comprehensive scoring results of propylene glycol addition amount

It can be seen from Table 5 that when the addition amount of propylene glycol is between 9% and 12%, the state is optimal. When it is lower than 9% or higher than 12%, the greasiness will become worse and the uniformity will also decrease.

(3) Screening of dosage of triethanolamine

Under the condition that the raw materials and other auxiliary materials are fixed, since triethanolamine will affect the pH of the gel, as shown in Table 6, the addition amount of triethanolamine is 1.2%, 1.5%, 1.8%, 2.1%, and 2.4. % is a variable, and the optimal addition amount is screened out.

Table 6 Triethanolamine addition amount design table

The five samples in Table 6 were examined from the five indicators of appearance, viscosity, uniformity, greasiness, and pH. The comprehensive scores are shown in Table 7.

Table 7 Comprehensive scoring results of triethanolamine addition amount

It can be seen from Table 7 that when the added amount of triethanolamine is higher than 1.8%, the pH can reach the standard suitable for the human body. However, when it is higher than 2.1%, the viscosity of the gel will increase and the uniformity will become worse. Therefore, the added amount of triethanolamine At 1.8% to 2.1%, the gel is in better condition.

(4) Orthogonal design of gel matrix

Based on the analysis of the results of the three single factors of the gel: carbomer 940, propylene glycol, and triethanolamine, an orthogonal experiment was designed for the gel matrix. The comprehensive evaluation results were used as the analysis index. The obtained orthogonal experiment results are: The optimal technological formula of Salicornia mosquito repellent and anti-itch gel. The orthogonal optimization factors and levels are shown in Table 8, and the L ₉ (3 ³ ) orthogonal analysis results are shown in Table 9.

Table 8 Orthogonal optimization factors and horizontal design table

Table 9 Orthogonal experiment analysis results

It can be seen from Table 9 that the degree of the three factors that affect the matrix of Salicornia gelatin is A>B>C, that is, the addition of carbomer 940 has the most significant impact on the Salicornia gelatin, followed by the addition of propylene glycol. Amount, triethanolamine has the least impact on Salicornia gel. The optimal solution for the samphire gel matrix is A ₂ B ₁ C ₂ , that is, adding 1.3% carbomer 940, 9% propylene glycol and 2.0% triethanolamine.

5. Verification experiment

According to the results of orthogonal optimization, three batches of gels were prepared by adding 1.3% carbomer 940, 9% propylene glycol and 2.0% triethanolamine to other raw materials and excipients, and the results were evaluated based on their appearance, viscosity, uniformity and greasiness. In sensory evaluation, the comprehensive scores are all between 58 and 60 points, which is relatively stable. The measured pH values of the three batches of gels are shown in Table 10. They are all between 6 and 8, which is in line with the acceptable pH index for the human body.

Table 10 pH of optimal prescription

Take 5g of each of these three batches of gelling agent and place it in a centrifuge tube, and centrifuge it for 20 minutes at 3000r·min ^-1 . It is observed that there is no obvious layering or color change in the sample, indicating that the stability of the sample is good.

Take 5g of each of these three batches of gels and place them in a centrifuge tube and place them at 60°C for 2 hours. No obvious layering or color change was observed in the samples, indicating that the samples have good heat resistance.

Take 5g of each of these three batches of gels and place them in a centrifuge tube and place them at -18°C for 2 hours. No obvious layering or color change was observed in the samples, indicating that the samples have good cold resistance.

Through the above verification experiments, it is shown that the process for preparing the samphire mosquito repellent and anti-itch gel is stable and feasible.

6. Methodological investigation

(1) Preparation of rutin standard solution

Accurately weigh 5 mg of rutin into a 50 mL volumetric flask, add 95% ethanol to adjust the volume to the mark, shake and shake well, and heat in a water bath until completely dissolved to obtain rutin with a concentration of 0.1 mg/mL. Standard solution.

(2) Preparation of test solution

Take 5g of the sample into a 50mL volumetric flask, add 95% ethanol to adjust the volume to the mark, shake well and put some part into a centrifuge tube, centrifuge at 3000rpm for 10min, then take 4mL of the supernatant into 10mL In the volumetric flask, add 0.3mL 5% NaNO ₂ solution, 0.3mL 10% Al(NO ₃ ) ₃ solution and 4mL 5% NaOH solution respectively, shake and shake well (each solution needs to stand for 6 minutes) , finally add 95% ethanol to adjust the volume to the mark, shake well, and let it stand for 15 minutes to obtain the test solution.

(3) Establishment and drawing of standard curve

Take 0 mL, 1.0 mL, 2.0 mL, 3.0 mL, 4.0 mL, and 5.0 mL of rutin standard solution into a 10 mL volumetric flask respectively. Add 0.3mL 5% NaNO ₂ solution, 0.3mL 10% Al(NO ₃ ) ₃ solution and 4mL 5% NaOH solution respectively, shake and shake well (each solution needs to stand for 6 minutes), and finally add 95% Dilute the volume of ethanol to the mark, shake well, and let stand for 15 minutes. Add 0 mL of rutin standard solution as a blank control, and measure the absorbance of the standard solution with a UV spectrophotometer at 510 nm. Taking the absorbance (c) of the rutin reference substance as the abscissa and the absorbance (A) as the ordinate, the linear regression equation is obtained: y=0.012x+0.0076, R ² =0.9991. The specific result data are shown in Table 11, and the rutin standard curve is shown in Figure 1.

Table 11 Determination of absorbance value of rutin standard curve

(4) Determination of total flavonoid content in Salicornia mosquito repellent and antipruritic gel

Take three samples and prepare them according to the method of the test solution. The absorbance is measured and then added to the rutin standard curve to obtain the rutin concentration in the sample. The total amount of flavonoids in the sample is calculated. The results are shown in Table 12.

Table 12 Determination of flavonoid content of samples

Example 3 Study on the Stability and Transdermal Effect of Samphire Mosquito Repellent and Antipruritic Gel

1. Study on the stability of Salicornia mosquito repellent and antipruritic gel

(1) Determination of storage stability of samples

Take a batch of samples (Samphire mosquito repellent and antipruritic gel prepared in Example 1) to prepare a test solution and place it under room temperature, cool and dark conditions, and use a blank sample without adding rutin standard solution as the test solution. For comparison, the absorbance of the samples stored for 0, 30, 60, 120, and 180 days was measured under the condition of 510 nm (Table 13). The results showed that the absorbance of the samples, that is, the flavonoid content, was relatively stable.

Table 13 Sample stability test results

(2) Determination of gel stability under extreme conditions

① Heat resistance test: Place the three batches of prepared samphire gel in a water bath and place it at 60°C for 7 days. Take samples at 1, 3, 5, and 7 days respectively to inspect various contents and take samples. The average values are recorded in the table and the results are shown in Table 14.

Table 14 High Temperature Experiment Results

② High humidity test: Place three batches of Salicornia gel at 25°C and relative humidity (90±5)% for 7 days, take samples at 1, 3, 5, and 7 days respectively, conduct various tests, and take the average The values are recorded in the table and the results are shown in 15.

Table 15 High Humidity Experiment Results

③Light test: Place three batches of Salicornia gel in a light incubator with a light intensity of (4500±500) LX for 7 days, take samples at 1, 3, 5, and 7 days respectively, examine various indicators, and average The values are recorded in the table and the results are shown in 16.

Table 16 Lighting experiment results

It can be seen from Tables 14-16 that the samphire mosquito repellent and antipruritic gel prepared in Example 1 has no significant change in the various detection indicators of the gel after high temperature and high humidity tests, and the properties of the gel in the light test are: The changes indicate that the samphire gel is sensitive to light and long-term direct exposure to strong light should be avoided. Experimental results show that high temperature has little effect on the stability of Salicornia gel, and will reduce the viscosity of the gel on the 7th day; humidity also has little effect on stability; light will cause oxidative deterioration of Salicornia gel , the color of the surface of the samphire gel became lighter on the 5th day, and turned slightly yellow on the 7th day.

2. Transdermal research on Samphire's mosquito repellent and antipruritic gel

(1) In vitro transdermal experiment

Fill the receiving chamber of the Franz diffusion cell with 30% ethanol saline, fix the mouse skin between the receiving chamber and the supply chamber of the diffusion cell, with the stratum corneum facing upward, and the skin should be in perfect contact with the liquid surface. The diffusion volume of the receiving chamber is 25 mL, the diffusion area is 0.785 cm ² , the mixture is in a water bath at 37°C, and the magnetic stirrer is stirred at a speed of 300 r/min. Add 2g of Salicornia mosquito repellent and antipruritic gel prepared in Example 1 to the supply chamber, and sample 1 mL from the receiving chamber at 2, 4, 6, 8, 10, and 12 hours respectively. Add 1 mL of 30% ethanol saline receiving solution. Filter the removed sample with a 0.22μm microporous filter membrane. According to the chromatographic detection conditions: ZORBAX SB-C18 (4.6×150mm, 5μm), the mobile phase is methanol-0.4% phosphoric acid (45:55), and the flow rate is 1.0mL/ min, the detection wavelength is 370nm, the column temperature is 30°C, the analysis time is 20min, and the injection volume is 10μL. The results are shown in Table 17 and Figure 2-4.

(2) Results and discussion

Table 17 In vitro transdermal results

It can be seen from Table 17 that the accumulated amount of rutin in Salicornia gel increases with time within 12 hours, and the increase rate is relatively fast in the first 4 hours, and then increases slowly.

It can be seen from Figure 2 and Figure 3 that the retention time of rutin is 4.5 minutes.

It can be seen from Figure 4 that the rutin content retained in the mouse skin after 12 hours was 34.114 μg·cm ^-2 , and the rutin content retained in the skin was relatively high.

The above experimental results show that the Salicornia mosquito repellent and anti-pruritic gel has good transdermal effect, can maintain a certain drug concentration in the subcutaneous tissue, and can achieve the effect of relieving itching and reducing swelling.

Example 4 Research on the Mosquito Repellent Effect of Samphire Mosquito Repellent and Antipruritic Gel

1. Materials and methods

The purchased Aedes albopictus was used as the research object. The feeding conditions were: temperature 28±2℃, relative humidity (70±5)%, photoperiod of 12h light and 12h dark environment, and 5% sucrose solution as feed. . The product is the samphire mosquito repellent and antipruritic gel prepared in Example 1.

In this embodiment, a Y-type olfactometer is used to analyze the attractiveness of Salicornia mosquito repellent and antipruritic gel to Aedes albopictus. The treatment group took different doses (0.2, 0.4, 0.8, 1.6g) of gel and smeared it on absorbent cotton, and placed it on the top of one branch of the Y-type olfactory analyzer. At the same time, the same amount of alcohol was used on the top of the other branch. For blank control, the experimental time is fixed every day, from 9 am to 9 pm. During the experiment, the ambient temperature was controlled to be 26±1°C, the relative humidity was (70±5)%, clean air was used as the carrier gas, and the flow rate was 0.02m ³ /h. Before the experiment, Aedes albopictus was put into the experimental environment for 30 minutes to adapt. Two tubes were connected to the tops of the two branches of the Y-type olfactory instrument to introduce air. 30 Aedes albopictus mosquitoes were put directly from the Y-type olfactory instrument. Put the mouth of the tube into the tube and let it adapt for 10 minutes. Then start timing and record the time for 20 minutes. Record the number of Aedes albopictus in the two Y branch ends. The experiment is repeated 6 times. The gel of the Y branch is replaced every three experiments. and alcohol positions, which must be washed and dried after each treatment to eliminate the impact of the position of the tube wall on mosquitoes. Repellency rate (%) = [(control group - treatment group)/(control group + treatment group)] × 100%. The experimental results are shown in Table 18.

2. Results and discussion

Table 18 Repellent effect of Samphire repellent and antipruritic gel against Aedes albopictus

As shown in Table 18, the samphire mosquito repellent and antipruritic gel prepared in Example 1 has a good repellent effect on Aedes albopictus. As the dose of the gel increases, the repellent effect is significant, showing a significant dose dependence. It can be seen from the results that when the gel dose is 0.2g, the mosquito repellent effect on Aedes albopictus has reached 65.71%, and when the dose reaches 1.6g, the mosquito repellent effect reaches 92.45%, indicating that the Samphire repellent and anti-itch gel The agent has significant mosquito repellent effect. The main ingredients of this gel come from plant extracts. It has the advantages of being safe for the environment, safe for natural enemies, not prone to drug resistance, and has a wide range of sources. It provides new ideas and ways for the development and preparation of mosquito repellent products on the market, and has important Theoretical and practical implications.

The above embodiments are only some of the embodiments of the present invention, not all of them. Based on the embodiments of the present invention, all other embodiments obtained by those skilled in the art without creative efforts should fall within the protection scope of the present invention.

Claims (7)

1. The Salicornia bigelovii mosquito-repellent itching-relieving gel is characterized by comprising, by weight, 20-25 parts of Salicornia bigelovii juice, 0.3-0.5 part of wormwood extract, 0.5-0.6 part of peppermint oil, 0.6-0.7 part of carbomer, 4-5 parts of propylene glycol, 4-5 parts of glycerol, 1-2 parts of pH regulator, 1-2 parts of penetration enhancer, 0.1 part of preservative and 18-20 parts of deionized water; wherein, the Salicornia bigelovii juice, the mugwort extract and the peppermint oil are gel raw materials; carbomers are gel matrices; propylene glycol and glycerin are humectants.

2. The Salicornia mosquito repellent antipruritic gel of claim 1, wherein the pH adjuster is triethanolamine, the penetration enhancer is azone, and the preservative is sodium benzoate.

3. The preparation method of the Salicornia bigelovii mosquito repellent itching relieving gel as claimed in claim 1 or 2, which is characterized by comprising the following steps:

step 1) pretreatment of raw materials: collecting fresh Salicornia bigelovii, cleaning, airing water for standby; collecting fresh aerial parts of mugwort, cleaning, air drying at room temperature, drying, and pulverizing; taking fresh peppermint, cleaning, drying and cutting into short sections for standby;

step 2) preparation of the salicornia juice: taking fresh Salicornia bigelovii that is drained, crushing, squeezing juice, filtering juice to remove residues, and adding vitamin C to obtain Salicornia bigelovii juice for standby;

step 3) preparation of the mugwort extract: by supercritical CO ₂ The preparation by fluid extraction technology, the moxa treated in the step 1) is uniformly filled in an extraction reaction kettle, and CO is utilized ₂ Repeatedly flushing to remove air in the pipeline; liquefying the CO ₂ Pumping into a mixer by a high-frequency plunger pump, regulating parameters, and preparing the wormwood extract for later use; step 4) preparation of peppermint oil: the peppermint oil is prepared by adopting a steaming method, extracting the peppermint raw materials according to the ratio of 1:8, keeping the micro-boiling state for more than 4 hours, and drying the prepared peppermint oil by using anhydrous sodium sulfateFiltering for standby;

step 5) preparation of the Salicornia bigelovii gel: weighing carbomer, propylene glycol, glycerol, triethanolamine, azone, sodium benzoate and deionized water according to parts by weight; uniformly scattering carbomer on the surface of deionized water, standing and swelling for 24 hours; sequentially adding corresponding amounts of propylene glycol, glycerol, azone, sodium benzoate, the Salicornia Herbacea juice, the folium Artemisiae Argyi extract and the peppermint oil prepared in step 1) to step 3) into a gel matrix, adding the next reagent after uniformly stirring the reagents with a mixer each time, and finally slowly adding distilled water and uniformly stirring to prepare the Salicornia Herbacea gel;

step 6) regulating and controlling the pH value of the Salicornia gel: adjusting the pH value of the Salicornia bigelovii gel prepared in the step 5) by using triethanolamine, uniformly stirring, and adjusting the pH value to 6-8;

and 7) performing hydroperoxide gel sterilization on the Salicornia bigelovii gel processed in the step 6) to obtain emerald transparent gel, and filling to obtain the Salicornia bigelovii mosquito-repellent itching-relieving gel finished product.

4. The method for preparing the Salicornia bigelovii mosquito repellent antipruritic gel according to claim 3, wherein the addition amount of the vitamin C in the step 2) is 0.5g/kg of Salicornia bigelovii.

5. The method for preparing the Salicornia bigelovii mosquito repellent antipruritic gel according to claim 3, wherein the setting of the control parameters in step 3) is as follows: controlling CO ₂ The flow rate is 25L/h, the separation pressure I of the reaction kettle is set to be 10+/-0.2 MPa, the temperature is 55 ℃, the separation pressure II is set to be 7+/-0.2 MPa, and the temperature is 55 ℃.

6. The method for preparing the Salicornia bigelovii mosquito repellent antipruritic gel according to claim 3, wherein the distilled water is added in the following amount in the step 5): the volume is fixed to 50 parts by weight.

7. The preparation method of the Salicornia bigelovii mosquito-repellent itching-relieving gel according to claim 3, wherein the weight of the Salicornia bigelovii mosquito-repellent itching-relieving gel finished product in the step 7) is 50g.