CN117327757A - Preparation process method of Melioserin-sea cucumber collagen peptide freeze-dried powder - Google Patents

Abstract

The invention discloses a preparation process method of a Meili active element-sea cucumber collagen peptide freeze-dried powder, which uses sea cucumber as an extraction material, firstly cuts open sea cucumber, removes viscera, washes with water, puts in dilute alkaline water to soak and remove the impurity protein on the sea cucumber, then dissolves collagen with acetic acid aqueous solution, then adds enzyme to hydrolyze, so that the collagen forms small molecular peptide, removes bitter peptide, then filters and decolors with active carbon, finally concentrates and freeze-dries in vacuum, the main characteristics of the process are good water solubility, and the normal temperature aqueous phase can be dissolved; no peculiar smell or bitter taste; and the waste low-value sea cucumbers can be fully utilized as raw materials, can be used as raw materials of nutritional foods, cosmetics and joint repair products, and has low product cost.

Description

Preparation process method of Melioserin-sea cucumber collagen peptide freeze-dried powder

Technical Field

The invention relates to the technical field of collagen peptide freeze-dried powder production, in particular to a preparation process method of a melilotin-sea cucumber collagen peptide freeze-dried powder.

Background

Collagen (Collagen) is a high molecular protein, which is the protein most abundant in animals, and accounts for about 25-33% of human protein, corresponding to 6% of human weight, and it is distributed throughout various organs of the whole body, such as bones, cartilage, ligaments, skin, cornea, various fascia, etc. Its main function is to maintain the morphology and structure of skin and tissue organs, and also is an important raw material substance for repairing various damaged tissues. 70% -80% of organic matters in bones are collagen, and when bones are generated, enough collagen fibers must be synthesized to form a framework of the bones. Thus, collagen is known as bone in bones. It is also the main component in skin and accounts for more than 71% of the protein content in skin cells. The growth, repair and nutrition of the skin are all independent of collagen. The collagen enables cells to become plump, so that skin is full, skin elasticity and luster are maintained, skin fineness and smoothness are maintained, wrinkles are stretched, texture and transparency are presented, and aging is effectively prevented. Two major keys to skin health, wrinkle resistance and moisture retention, are both related to collagen. And also has obvious effect in repairing skin.

Collagen peptides widely used in foods, cosmetics and beverages as additives are derived from collagen, and the main sources of collagen are animal skin, bone, tendon, placenta, etc., and collagen sources of nursing animals are greatly impacted due to the continuous occurrence of diseases such as mad cow disease, mad sheep disease, etc. by invasion of pathogenic bacteria, so that it is necessary to consider to replace and prepare collagen peptides with new resources by new processes.

Sea cucumber is a generic term for animals of the class Stichopus japonicus of the phylum Echinodermata. Sea cucumber is commonly called as sea ginseng. Since ancient times, mountain ginseng and sea cucumber are almost named. The Ming dynasty "five miscellaneous groups" describes: sea cucumber is warm and tonic, and is a natural enemy of ginseng, so it is a sea cucumber. Sea cucumber is classified as a precious good product by rich nutrition, and is one of the famous "eight delicacies of sea taste" in China. It has the special effects of curing diseases of kidney deficiency, impotence, constipation due to intestinal dryness, phthisis, duodenal ulcer, poliomyelitis, epilepsy, etc., and has the functions of curing human body growth, curing wound, resisting inflammation, resisting cold, preventing tissue ageing, arteriosclerosis and diabetes, etc., and is a medicine and edible product with wider anti-tumor spectrum. Compendium of materia Medica initial Yi: taste Gan Cheng, tonify kidney meridian, nourish essence and marrow, relieve urination, strengthen yang and treat spasm. Diet Spectrum with rest: "nourishing yin, tonifying blood, strengthening yang, moistening dryness, regulating menstruation, nourishing fetus, and benefiting yield". The traditional Chinese medicine considers that the sea cucumber has the effects of tonifying kidney, replenishing vital essence, nourishing blood, moistening lung, relieving convulsion, calming heart, stopping bleeding, diminishing inflammation, relieving acid, stopping pain, nourishing brain and improving intelligence. Is mainly used for treating consumptive disease, weakness and ulcer, dry stool, dizziness and tinnitus, frequent urination, soreness of waist and debilitation, impotence, postpartum constipation, nocturnal emission and spermatorrhea, and the like. It is known that coastal fishermen think that people eat a sea cucumber every day, and the people can fully feel red light within one month, are full of energy, and can also treat coronary heart disease and cold resistance.

Modern medical research has considered that: the rich protein in sea cucumber is an important nutrient component. The sea cucumber has thorns and no thorns, and the protein of the sea cucumber accounts for more than 60 percent. Sea cucumber proteins mainly consist of collagen and elastin. The composition of the amino acid is various, including 20 kinds of glycine, glutamic acid, aspartic acid, alanine and the like, and the amino acid is rich in 8 kinds of essential amino acids which cannot be manufactured and synthesized by human bodies.

There are 1100 kinds of sea cucumbers, which are generally classified into thorns and no thorns, 100 kinds of sea cucumbers exist in the sea area of China, and 20 kinds of sea cucumbers can be eaten. Research shows that sea cucumber resources as marine organisms are rich, especially sea cucumbers in temperate and tropical seas are few to eat due to no thorns or bad taste, and development and application are needed.

The collagen peptide is prepared by utilizing low-value sea cucumbers with abundant resources and adopting an enzymolysis technology, and the water-soluble freeze-dried powder is formed by utilizing a freeze-drying technology, so that the collagen peptide has very broad market development and application prospects, and provides raw materials for preparing nutritional foods, cosmetics and repairing joint products.

Disclosure of Invention

In order to solve the technical problems, the invention adopts the following technical scheme: the invention aims to fully utilize low-value sea cucumbers as collagen resources and provide a process method for producing sea cucumber collagen peptide freeze-dried powder-melilotin.

The invention uses sea cucumber as extraction material, firstly dissects sea cucumber, removes viscera, washes with water, puts in dilute alkali water to soak and remove the impurity protein on the sea cucumber, then dissolves collagen with acetic acid water solution, then adds enzyme to hydrolyze, makes collagen form micromolecular peptide, removes bitter peptide, filters and decolors with active carbon, finally concentrates, freezes and dries in vacuum, thus obtaining sea cucumber collagen peptide freeze-dried powder-meracil.

The dilute alkaline water can be NaOH or NaHCO ₃ The weight ratio of the acetic acid (HAc) to water is 0.5-1%, and the concentration of the acetic acid (HAc) in the water solution is 0.5-1%; the enzyme-added hydrolysis is to add food enzyme and flavor enzyme successively, and the weight ratio of the enzyme to the skin ginseng is preferably 1:100.

The method comprises the following steps:

1. pi Can, manufacturing: cutting sea cucumber and removing viscera;

2. pi Can washing: removing viscous impurity protein by using dilute alkali;

3. pi Can hydrolysis: using dilute acid sol;

4. the enzymolysis comprises the steps of firstly hydrolyzing with compound food enzyme, and then removing bitter peptide with flavor enzyme;

5. decoloring: adsorbing and decoloring by using active carbon;

6. and (3) filtering: filtering with 5u pulp board;

7. and (3) freeze drying: lyophilizing with vacuum freeze dryer;

8. and (3) pulverizing and packaging: pulverizing, and vacuum packaging.

The process has the main characteristics of good water solubility, and can be dissolved in water phase at normal temperature; no peculiar smell or bitter taste; and the waste low-value sea cucumbers can be fully utilized as raw materials, can be used as raw materials of nutritional foods, cosmetics and joint repair products, and has low product cost.

Detailed Description

The technical solutions in the embodiments of the present invention are clearly and completely described, and it is obvious that the described embodiments are only some embodiments of the present invention, but not all embodiments. All other embodiments, which can be made by those skilled in the art based on the embodiments of the invention without making any inventive effort, are intended to be within the scope of the invention.

The invention uses sea cucumber as extraction material, firstly dissects sea cucumber, removes viscera, washes with water, puts in dilute alkali water to soak and remove the impurity protein on the sea cucumber, then dissolves collagen with acetic acid water solution, then adds complex enzyme to hydrolyze, makes collagen form micromolecular peptide, removes bitter peptide with flavor enzyme, then uses active carbon to filter and decolor, finally concentrates, freezes and dries in vacuum, thus obtaining sea cucumber collagen peptide-meracil.

The dilute alkaline water can be NaOH or NaHCO ₃ The weight ratio of the acetic acid (HAc) to water is 0.5-1%, and the concentration of the acetic acid (HAc) in the water solution is 0.5-1%; the enzyme-added hydrolysis is to add food enzyme and flavor enzyme successively, and the weight ratio of the enzyme to the skin ginseng is preferably 1:100.

The method comprises the following specific steps:

1. pi Can, manufacturing: cutting sea cucumber, removing viscera, cleaning with water, and taking out to obtain skin ginseng;

2. pi Can washing: soaking radix Ginseng Rubra in 1%NaOH water solution for 24 hr, repeating for at least three times continuously to remove impurity proteins from radix Ginseng Rubra;

3. pi Can hydrolysis: placing the processed radix Ginseng Rubra in a container containing 1% HAc aqueous solution (weight of radix Ginseng involves in acid liquor, etc.), and dissolving at 40-50deg.C;

4. enzymolysis: the glue solution is hydrolyzed by composite proteinase. Hydrolysis conditions: the enzyme addition amount is 1% of the weight of the skin ginseng, the hydrolysis time is 1-2 hours, and the temperature is 35-40 ℃. Ph7-8, then hydrolyzed with flavourzyme, hydrolysis conditions: the enzyme addition amount is 1% of the weight of the ginseng, the hydrolysis time is 1 hour, and the temperature is 35-37 ℃. Ph7-7.5, and heating to 85-100deg.C for sterilization.

5. Decoloring: and (3) decoloring the hydrolysate by using activated carbon under stirring for 2-3 hours.

6. And (3) filtering: filtration was performed with a 5u pulp board frame.

7. And (3) freeze-drying: spreading the filtrate on a tray, pre-cooling in a refrigerator below 5 ℃ and deep freezing below-20 ℃; and (3) loading the frozen sample into a freeze dryer, freezing in vacuum at the temperature of minus 54 ℃ and the vacuum degree of 5mtorr, and freeze-drying. And (3) taking out the dry powder after 16 hours until the sample reaches constant weight.

8. And (3) pulverizing and packaging: pulverizing with pulverizer, and vacuum sealing and packaging the lyophilized powder.

It will be apparent to those skilled in the art that various modifications and variations can be made to the present invention without departing from the spirit or scope of the invention. Thus, it is intended that the present invention also include such modifications and alterations insofar as they come within the scope of the appended claims or the equivalents thereof.

Claims (1)

1. A preparation process method of a Meliosin-sea cucumber collagen peptide freeze-dried powder is characterized by comprising the following steps: firstly cutting sea cucumber, removing viscera, washing with water, soaking in dilute alkaline water to remove impurity proteins on the sea cucumber, dissolving collagen in aqueous acetic acid solution, adding enzyme to hydrolyze the collagen to form micromolecular peptide, removing bitter peptide, filtering with active carbon to decolorize, concentrating, and vacuum freeze-drying to obtain sea cucumber collagen peptide-meracil;

the dilute alkaline water can be NaOH or NaHCO ₃ The weight ratio of the acetic acid (HAc) to water is 0.5-1%, and the concentration of the acetic acid (HAc) in the water solution is 0.5-1%; the enzyme-added hydrolysis is to add food enzyme and flavor enzyme successively, and the weight ratio of the enzyme to the skin ginseng is preferably 1:100.