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CN103976203A - Jelly capable of maintaining beauty and keeping young and preparation method thereof - Google Patents

Jelly capable of maintaining beauty and keeping young and preparation method thereof Download PDF

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CN103976203A
CN103976203A CN201410238237.3A CN201410238237A CN103976203A CN 103976203 A CN103976203 A CN 103976203A CN 201410238237 A CN201410238237 A CN 201410238237A CN 103976203 A CN103976203 A CN 103976203A
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CN103976203B (en
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张志强
候长红
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Shandong Haiyu Biotechnology Co ltd
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Shandong Hai Yu Bioisystech Co Ltd
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    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23LFOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES, NOT OTHERWISE PROVIDED FOR; PREPARATION OR TREATMENT THEREOF
    • A23L33/00Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
    • A23L33/10Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
    • A23L33/105Plant extracts, their artificial duplicates or their derivatives
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23LFOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES, NOT OTHERWISE PROVIDED FOR; PREPARATION OR TREATMENT THEREOF
    • A23L21/00Marmalades, jams, jellies or the like; Products from apiculture; Preparation or treatment thereof
    • A23L21/10Marmalades; Jams; Jellies; Other similar fruit or vegetable compositions; Simulated fruit products
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23VINDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2002/00Food compositions, function of food ingredients or processes for food or foodstuffs

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  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)

Abstract

本发明公开了一种美容养颜的果冻及其制备方法,果冻原料为:骨胶原蛋白100份、透明质酸10-15份、蜂王浆15-20份、葡萄糖胺20-25份、香菇15-20份、猴头菇5-10份、土瓜根5-10份、栗子内果皮15-20份、茯苓10-15份、芝麻5-10份、草莓20-25份、芹菜15-20份、槐花1-5份、红曲5-10份、玉米10-15份、麦麸5-10份、红豆5-10份、海藻10-15份、鱼骨1-5份、鱼皮1-5份、鱼鳞1-5份、葡萄糖酸钙1-5份、葡萄糖酸锌0.1-0.3份。本发明配方独特,将胶原蛋白制成果冻状,服用和携带更加方便。通过各成分的协同作用,具有很好的美容养颜和保健作用。The invention discloses a jelly for beauty and beauty and a preparation method thereof. The raw materials of the jelly are: 100 parts of bone collagen, 10-15 parts of hyaluronic acid, 15-20 parts of royal jelly, 20-25 parts of glucosamine, and 15-20 parts of shiitake mushrooms. Hericium erinaceus 5-10 parts, melon root 5-10 parts, chestnut endocarp 15-20 parts, Poria cocos 10-15 parts, sesame 5-10 parts, strawberry 20-25 parts, celery 15-20 parts, Sophora japonica 1-5 parts, red yeast rice 5-10 parts, corn 10-15 parts, wheat bran 5-10 parts, red beans 5-10 parts, seaweed 10-15 parts, fish bones 1-5 parts, fish skin 1- 5 parts, fish scale 1-5 parts, calcium gluconate 1-5 parts, zinc gluconate 0.1-0.3 parts. The invention has a unique formula, and the collagen is made into a jelly shape, which is more convenient to take and carry. Through the synergistic effect of each component, it has good beauty care and health care effects.

Description

一种美容养颜的果冻及其制备方法A kind of jelly for beauty and beauty and preparation method thereof

技术领域 technical field

本发明涉及一种含有胶原蛋白的保健食品,具体涉及一种具有美容养颜功效的可食用的胶原蛋白果冻及其制备方法,属于食品和保健品技术领域。 The invention relates to a health food containing collagen, in particular to an edible collagen jelly with beauty and beauty effects and a preparation method thereof, belonging to the technical field of food and health products.

背景技术 Background technique

果冻是一种西方食品,它一般由增稠剂(海藻酸钠、琼脂、食用明胶、卡拉胶等)并加入各营养成分、香精、着色剂、甜味剂等制备而成。果冻虽然外观可爱,但一般情况下其所含有的营养物质过于缺乏,因此近年来果冻的成份中又增加了牛奶、巧克力以及其它一些营养物质,从而在满足人们口欲的情况下,也不至于导致营养缺乏。 Jelly is a western food, which is generally prepared by thickeners (sodium alginate, agar, edible gelatin, carrageenan, etc.) and adding various nutrients, flavors, colorants, sweeteners, etc. Although the appearance of jelly is cute, its contained nutrients are generally too lacking. Therefore, in recent years, milk, chocolate and some other nutrients have been added to the composition of jelly, so that it will not be too much when satisfying people's appetite. lead to nutritional deficiencies.

胶原蛋白(Collagen)是动物体内含量最丰富的蛋白质,它属于不溶性纤维型蛋白质,也是细胞外基质中的一类,主要存在于结缔组织中。它具有很强的伸张能力,是韧带和肌键的主要成份,还是细胞外基质的主要组成成分。胶原蛋白在人体内有重要的作用,例如:保湿、滋养、亮肤、紧肤、防皱等美肤作用,还具有丰胸、保养头发、防癌抗癌、保持眼角膜透明等作用,是女性保持健康、美丽的重要成分。 Collagen is the most abundant protein in animals. It is an insoluble fibrous protein and a type of extracellular matrix, mainly found in connective tissue. It has a strong stretching ability, is the main component of ligaments and muscle bonds, and is also the main component of extracellular matrix. Collagen plays an important role in the human body, such as: moisturizing, nourishing, skin brightening, firming, anti-wrinkle and other skin beautification effects. It also has breast enhancement, hair maintenance, cancer prevention and anti-cancer, and keeps the cornea transparent. Important ingredients to maintain health and beauty.

女性年龄超过25岁后,身体内的胶原蛋白就会大量流失,随之肌肤会出现毛孔粗大、皮肤暗沉、色斑、皱纹等问题。为了保持肌肤弹性,口服胶原蛋白已经成为现今流行的保健产品。目前,胶原蛋白一般是胶囊、颗粒或者粉剂,虽然服用较简单,但是口感一般,且保健作用较为单一,美容效果起效慢。 When a woman is over 25 years old, a large amount of collagen in the body will be lost, followed by problems such as enlarged pores, dull skin, pigmentation, and wrinkles. In order to maintain skin elasticity, oral collagen has become a popular health care product nowadays. At present, collagen is generally in the form of capsules, granules or powders. Although it is relatively simple to take, the taste is average, and the health care effect is relatively simple, and the beauty effect is slow to take effect.

发明内容 Contents of the invention

本发明的目的是提供一种美容养颜的果冻,其口感酸甜爽滑,服用和携带方便,且营养丰富,能促进人体更好地吸收胶原蛋白,快速起到美容养颜的作用。 The object of the present invention is to provide a jelly for beautifying and beautifying the skin, which has a sweet and sour taste, is convenient to take and carry, and is rich in nutrition, can promote better absorption of collagen by the human body, and quickly play the role of beautifying and beautifying the skin.

本发明的另一目的是提供该果冻的制备方法,该方法简单易行,将各原料特殊加工,充分提取了原料中的营养成分,使其口感、营养更佳,且产品成功率高,便于工业化大生产。 Another object of the present invention is to provide a preparation method of the jelly, which is simple and easy, and the raw materials are specially processed to fully extract the nutritional components in the raw materials, so that the taste and nutrition are better, and the product has a high success rate, which is convenient Industrialized mass production.

本发明产品主要针对女性消费者,通过以下技术方案实现: The product of the present invention is mainly aimed at female consumers, and is realized through the following technical solutions:

一种美容养颜的果冻,包括以下重量份的原料:骨胶原蛋白100份、透明质酸10-15份、蜂王浆15-20份、葡萄糖胺20-25份、香菇15-20份、猴头菇5-10份、土瓜根5-10份、栗子内果皮15-20份、茯苓10-15份、芝麻5-10份、草莓20-25份、芹菜15-20份、槐花1-5份、红曲5-10份、玉米10-15份、麦麸5-10份、红豆5-10份、海藻10-15份、鱼骨1-5份、鱼皮1-5份、鱼鳞1-5份、葡萄糖酸钙1-5份、葡萄糖酸锌0.1-0.3份。 A jelly for beauty and beauty, comprising the following raw materials in parts by weight: 100 parts of bone collagen, 10-15 parts of hyaluronic acid, 15-20 parts of royal jelly, 20-25 parts of glucosamine, 15-20 parts of shiitake mushroom, Hericium erinaceus 5-10 parts, 5-10 parts of soil melon root, 15-20 parts of chestnut endocarp, 10-15 parts of poria cocos, 5-10 parts of sesame, 20-25 parts of strawberry, 15-20 parts of celery, 1-5 parts of Sophora japonica 5-10 parts of red yeast rice, 10-15 parts of corn, 5-10 parts of wheat bran, 5-10 parts of red beans, 10-15 parts of seaweed, 1-5 parts of fish bones, 1-5 parts of fish skin, and 1 part of fish scale -5 parts, 1-5 parts of calcium gluconate, 0.1-0.3 parts of zinc gluconate.

上述配方中,各原料按照特殊比例组合,效果最佳,优选包括以下重量份的原料:骨胶原蛋白100份、透明质酸12份、蜂王浆18份、葡萄糖胺22份、香菇16份、猴头菇6份、土瓜根6份、栗子内果皮16份、茯苓12份、芝麻6份、草莓24份、芹菜18份、槐花2份、红曲6份、玉米14份、麦麸6份、红豆6份、海藻14份、鱼骨3份、鱼皮3份、鱼鳞3份、葡萄糖酸钙2份、葡萄糖酸锌0.2份。 In the above formula, each raw material is combined according to a special ratio, and the effect is the best, preferably including the following raw materials in parts by weight: 100 parts of bone collagen, 12 parts of hyaluronic acid, 18 parts of royal jelly, 22 parts of glucosamine, 16 parts of shiitake mushrooms, Hericium erinaceus 6 parts of mushroom, 6 parts of soil melon root, 16 parts of chestnut inner peel, 12 parts of poria cocos, 6 parts of sesame, 24 parts of strawberry, 18 parts of celery, 2 parts of Sophora japonica, 6 parts of red yeast rice, 14 parts of corn, 6 parts of wheat bran , 6 parts of red beans, 14 parts of seaweed, 3 parts of fish bones, 3 parts of fish skin, 3 parts of fish scales, 2 parts of calcium gluconate, and 0.2 parts of zinc gluconate.

上述配方中,原料中还可以加入调味剂。 In the above formula, flavoring agents can also be added to the raw materials.

上述配方中,所用透明质酸分子量为5000-10000道尔顿。 In the above formula, the molecular weight of hyaluronic acid used is 5000-10000 Daltons.

上述配方中,所用鱼骨、鱼皮和鱼鳞优选为咸水鱼的鱼骨、鱼鳞和鱼皮,例如罗非鱼、黄花鱼、带鱼等。 In the above formula, the fish bones, fish skin and fish scales used are preferably the fish bones, fish scales and fish skins of saltwater fish, such as tilapia, yellow croaker, hairtail and the like.

本发明美容养颜的果冻的制备方法,包括以下步骤: The preparation method of the jelly for beautifying and nourishing skin of the present invention comprises the following steps:

(1)将香菇、猴头菇、土瓜根、栗子内果皮、茯苓粉碎成细粉,加入其质量3-5倍的水,微波提取2-3h; (1) Grind shiitake mushrooms, Hericium erinaceus, soil melon root, chestnut endocarp, and poria cocos into fine powder, add water 3-5 times its mass, and microwave for 2-3 hours;

(2)微波提取后过滤,向提取液中加入木瓜蛋白酶和纤维素酶,在50-60℃下酶解0.5-1h,酶解以后进行冷灭酶,过滤,取滤液备用; (2) After microwave extraction, filter, add papain and cellulase to the extract, enzymatically hydrolyze at 50-60°C for 0.5-1h, after enzymolysis, cold inactivate the enzyme, filter, and take the filtrate for later use;

(3)将草莓、芹菜切成小块,与芝麻、槐花混合,加入它们质量3-4倍的水榨成汁,向所得汁液中加入葡萄糖酸钙和葡萄糖酸锌,搅拌均匀,然后按照3-5%的接种量加入混合菌,在28-35℃下发酵10-12h,发酵完后过滤,取滤液备用; (3) Cut strawberries and celery into small pieces, mix them with sesame seeds and Sophora japonica, add water 3-4 times their mass to squeeze out juice, add calcium gluconate and zinc gluconate to the obtained juice, stir well, and then follow Add 3-5% of the inoculum to the mixed bacteria, ferment at 28-35°C for 10-12 hours, filter after fermentation, and take the filtrate for later use;

(4)将玉米、海藻、鱼骨、鱼皮、鱼鳞洗净、切碎,红豆磨成粗粉,然后将它们与麦麸、红曲混合,在混合物中加入它们质量6-8倍的水,加热水煮,水沸腾15min后停止,混合液温度降至35-50℃时加入纤维素酶、果胶酶和淀粉酶,酶解0.5-1h,酶解以后进行冷灭酶;然后将温度控制在30-45℃,向混合液中加入木瓜蛋白酶、胰蛋白酶和脂肪酶,酶解1.5-2.5h,酶解以后进行冷灭酶,过滤; (4) Wash and chop corn, seaweed, fish bones, fish skin, and fish scales, and grind red beans into coarse powder, then mix them with wheat bran and red yeast rice, and add water 6-8 times their mass in the mixture , heat and boil in water, stop after the water boils for 15 minutes, add cellulase, pectinase and amylase when the temperature of the mixture drops to 35-50°C, enzymatic hydrolysis for 0.5-1h, and then cool the enzyme after enzymatic hydrolysis; then lower the temperature Control at 30-45°C, add papain, trypsin and lipase to the mixed solution, enzymatic hydrolysis for 1.5-2.5h, after enzymatic hydrolysis, cold-kill the enzyme, and filter;

(5)将步骤(4)的滤液按照5-8%的接种量接种酵母菌,在25-30℃发酵3-4h,过滤取滤液备用; (5) Inoculate the filtrate of step (4) with yeast according to the inoculum amount of 5-8%, ferment at 25-30°C for 3-4 hours, filter and take the filtrate for later use;

(6)将步骤(2)、(3)、(5)的溶液混合,加入真空浓缩机中,低温真空浓缩至原体积的2/3; (6) Mix the solutions of steps (2), (3), and (5), add them to a vacuum concentrator, and concentrate them in vacuum at low temperature to 2/3 of the original volume;

(7)向浓缩液中加入骨胶原蛋白、透明质酸、蜂王浆、葡萄糖胺,加热至50-60℃使各成分充分溶解,然后将溶液倒入模具中,冷却后即得果冻。 (7) Add bone collagen, hyaluronic acid, royal jelly, and glucosamine to the concentrated solution, heat to 50-60°C to fully dissolve the ingredients, then pour the solution into the mold, and get the jelly after cooling.

步骤(1)中,采用微波提取,使有效成分可以更为快速、全面的溶于水中。微波功率为15-20w,微波频率为2450 MHz,提取温度为80-100℃。 In step (1), microwave extraction is used, so that the active ingredients can be dissolved in water more quickly and comprehensively. The microwave power is 15-20w, the microwave frequency is 2450 MHz, and the extraction temperature is 80-100°C.

步骤(2)中,通过酶的作用,将大分子物质进行降解,更便于人体吸收。木瓜蛋白酶用量为提取液的1.5‰,纤维素酶的用量为提取液的3.5‰。 In step (2), macromolecular substances are degraded through the action of enzymes, which is more convenient for human body to absorb. The dosage of papain is 1.5‰ of the extract, and the dosage of cellulase is 3.5‰ of the extract.

步骤(3)中,将草莓等原料用混合菌进行发酵,经过发酵除去了对人体不利的成分,发酵后的产物更便于吸收。所用混合菌为酵母菌、双歧杆菌和红曲霉菌的混合物,酵母菌:双歧杆菌:红曲霉菌的质量比为2.5:1:4.3。 In step (3), strawberries and other raw materials are fermented with mixed bacteria, and the ingredients that are harmful to the human body are removed through fermentation, and the fermented products are easier to absorb. The mixed bacteria used is a mixture of yeast, bifidobacteria and monascus, and the mass ratio of yeast: bifidobacteria: monascus is 2.5:1:4.3.

步骤(4)中,通过加热对原料进行除味,通过酶的作用,将大分子物质进行降解,更便于人体吸收。纤维素酶的用量为混合液质量的0.5‰、果胶酶的用量为混合液质量的0.8‰,淀粉酶的用量为混合液质量的1.5‰;木瓜蛋白酶的用量为混合液质量的3.5‰、胰蛋白酶的用量为混合液质量的1.8‰,脂肪酶的用量为混合液质量的0.8‰。 In step (4), the raw materials are deodorized by heating, and the macromolecular substances are degraded by the action of enzymes, which is more convenient for human body to absorb. The consumption of cellulase is 0.5‰ of the quality of the mixed solution, the consumption of pectinase is 0.8‰ of the quality of the mixed solution, the consumption of amylase is 1.5‰ of the quality of the mixed solution; the consumption of papain is 3.5‰ of the quality of the mixed solution, The dosage of trypsin is 1.8‰ of the quality of the mixture, and the dosage of lipase is 0.8‰ of the quality of the mixture.

步骤(5中,通过发酵进一步去除原料中的腥味。 In step (5), the fishy smell in the raw material is further removed by fermentation.

步骤(6)中,浓缩温度为50-65℃,真空度为0.095-0.1MPa。 In step (6), the concentration temperature is 50-65° C., and the vacuum degree is 0.095-0.1 MPa.

步骤(2)和(4)中,冷灭酶方法为:在室温下,250-300MPa压力下灭酶4-7分钟。 In steps (2) and (4), the method of cold inactivating the enzyme is: inactivate the enzyme at room temperature and under a pressure of 250-300 MPa for 4-7 minutes.

本发明突破传统理念,将胶原蛋白制成果冻状,选择骨胶原蛋白作为胶原蛋白的原料,其为低份子胶原蛋白,有助吸收,能补充胶原蛋白流失,扩大细胞储水空间,为肌肤补充水份,柔化肌肤细胞,令肌肤更弹性、水润、光泽、紧致 。此外,配方中含有多种对人体有益的成分,通过它们之间的协同起效,能提供较全面的营养,同时起到很好的美容、保健的作用。 The present invention breaks through the traditional idea, makes collagen into jelly, and chooses bone collagen as the raw material of collagen, which is low-molecular-weight collagen, which can help absorption, supplement collagen loss, expand cell water storage space, and replenish skin. Moisture, softens skin cells, making skin more elastic, moist, shiny and firm. In addition, the formula contains a variety of ingredients that are beneficial to the human body. Through their synergistic effect, they can provide more comprehensive nutrition, and at the same time play a very good role in beauty and health care.

本发明透明质酸为低份子透明质酸,能提高肌肤锁水度,防止水份流失,改善干燥及皱纹等问题,维持肌肤弹性及紧致。葡萄糖胺能加速软骨细胞的再生与修复,提升皮肤弹力和光泽度 。香菇、猴头菇、土瓜根、栗子内果皮、茯苓中含有对人体有益的营养成分,尤其是能够保肝护肝,具有很好的祛斑作用。葡萄糖酸钙、葡萄糖酸锌能补充人体重要的微量元素成分。此外,芝麻、草莓、芹菜、槐花、红曲、玉米、麦麸、红豆、海藻、鱼骨、鱼皮、鱼鳞、蜂王浆中含有大量的维生素、氨基酸、微量元素、膳食纤维、多糖、碳水化合物、抗氧化等成分,能够防止细胞氧化,增强细胞活力,提高人体免疫力。 The hyaluronic acid of the present invention is a low-molecular-weight hyaluronic acid, which can improve skin moisture retention, prevent moisture loss, improve dryness and wrinkles, and maintain skin elasticity and firmness. Glucosamine can accelerate the regeneration and repair of cartilage cells, and improve skin elasticity and gloss. Lentinus edodes, Hericium erinaceus, soil melon root, chestnut endocarp, and poria cocos contain nutrients that are beneficial to the human body, especially can protect the liver and liver, and have a good freckle removal effect. Calcium gluconate and zinc gluconate can supplement the important trace elements of the human body. In addition, sesame, strawberry, celery, Sophora japonica, red yeast rice, corn, wheat bran, red bean, seaweed, fish bone, fish skin, fish scale, royal jelly contain a lot of vitamins, amino acids, trace elements, dietary fiber, polysaccharides, carbohydrates , anti-oxidation and other ingredients can prevent cell oxidation, enhance cell vitality, and improve human immunity.

本发明采用特殊的制备方法对原料进行处理,很好的提取了原料中的有用成分,并通过处理去除了原料中的腥、苦等异味,口感更佳。此外,用各种菌株或酶对原料进行处理,使原料中的有效成分更易于被人体快速吸收,效果更好。 The invention adopts a special preparation method to process the raw materials, and the useful components in the raw materials are well extracted, and the fishy, bitter and other peculiar smells in the raw materials are removed through the treatment, and the taste is better. In addition, the raw materials are treated with various bacterial strains or enzymes, so that the active ingredients in the raw materials are more easily absorbed by the human body and the effect is better.

本发明优点为: Advantage of the present invention is:

1、本发明配方独特,将胶原蛋白制成果冻状,辅以其他成分,口感更佳,更易于被消费者接受,服用和携带更加方便。通过各成分的协同作用,本发明产品可以起到增强免疫力、排毒、养胃、保肝、通便、修复、抗氧化等作用,具有很好的美容养颜和保健作用。 1. The formula of the present invention is unique. The collagen is made into a jelly shape and supplemented with other ingredients. The taste is better, it is easier to be accepted by consumers, and it is more convenient to take and carry. Through the synergistic effect of each component, the product of the invention can enhance immunity, detoxify, nourish the stomach, protect the liver, laxative, repair, and resist oxidation, and has good beauty and health effects.

2、本发明以胶原蛋白为主,因为胶原蛋白的性能,产品中无需加入卡拉胶、明胶等增稠剂即可制成果冻,成型性好。 2. The present invention is mainly based on collagen, because of the properties of collagen, the product can be made into jelly without adding thickeners such as carrageenan and gelatin, and has good formability.

3、本发明以骨胶原蛋白作为胶原蛋白的原料,被人体食用后不会受到人体各种酶及酸碱物质水解,直接进入小肠,被小肠所吸收,快速进入人体血液循环系统,发挥生物学功能。 3. The present invention uses bone collagen as the raw material of collagen. After being eaten by the human body, it will not be hydrolyzed by various enzymes and acid-base substances in the human body. It will directly enter the small intestine, be absorbed by the small intestine, and quickly enter the human blood circulation system to exert biological Function.

4、本发明能从整体上提高人体的细胞活性,提高人体免疫力,尤其是对便秘、色斑、皮肤暗沉、皮肤缺水人群有特效,能够美白亮肤、防皱紧肤、修复肌肤、加速角质层的更新、补水锁水。 4. The present invention can improve the cell activity of the human body as a whole, improve the immunity of the human body, especially have special effects on people with constipation, pigmentation, dull skin, and dehydrated skin, and can whiten and brighten the skin, prevent wrinkles and firm the skin, and repair the skin , Accelerate the renewal of the stratum corneum, replenish water and lock water.

具体实施方式 Detailed ways

下面,通过具体实施例对本发明进行进一步的说明,应该明白的是,下述说明仅是为了解释本发明,并不对其内容进行限定。如无特殊说明,下述实施例所用原料均为重量份。 Below, the present invention will be further described through specific examples. It should be understood that the following description is only for explaining the present invention, and not limiting its content. Unless otherwise specified, the raw materials used in the following examples are all parts by weight.

本发明所用骨胶原蛋白购自湖州珍露生物制品有限公司;所用的纤维素酶、果胶酶、淀粉酶、脂肪酶选自宁夏和氏璧生物技术有限公司;所用的木瓜蛋白酶、胰蛋白酶选自上海楷洋生物技术销售总公司。所用菌种购自通派(上海)生物科技有限公司。 The bone collagen used in the present invention is purchased from Huzhou Zhenlu Biological Products Co., Ltd.; used cellulase, pectinase, amylase, and lipase are selected from Ningxia Heshibi Biotechnology Co., Ltd.; used papain and trypsin are selected from From Shanghai Kaiyang Biotechnology Sales Corporation. The strains used were purchased from Tongpai (Shanghai) Biotechnology Co., Ltd.

下述实施例中,所用透明质酸分子量在5000-10000道尔顿之间。 In the following examples, the molecular weight of hyaluronic acid used is between 5000-10000 Daltons.

实施例1Example 1

按照以下配方选取原料:骨胶原蛋白100份、透明质酸12份、蜂王浆18份、葡萄糖胺22份、香菇16份、猴头菇6份、土瓜根6份、栗子内果皮16份、茯苓12份、芝麻6份、草莓24份、芹菜18份、槐花2份、红曲6份、玉米14份、麦麸6份、红豆6份、海藻14份、鱼骨3份、鱼皮3份、鱼鳞3份、葡萄糖酸钙2份、葡萄糖酸锌0.2份。 Select raw materials according to the following formula: 100 parts of bone collagen, 12 parts of hyaluronic acid, 18 parts of royal jelly, 22 parts of glucosamine, 16 parts of shiitake mushrooms, 6 parts of Hericium erinaceus, 6 parts of soil melon root, 16 parts of chestnut endocarp, Poria cocos 12 parts, 6 parts of sesame, 24 parts of strawberry, 18 parts of celery, 2 parts of Sophora japonica, 6 parts of red yeast rice, 14 parts of corn, 6 parts of wheat bran, 6 parts of red bean, 14 parts of seaweed, 3 parts of fish bone, 3 parts of fish skin 2 parts, 3 parts of fish scales, 2 parts of calcium gluconate, 0.2 parts of zinc gluconate.

采用以下方法制备果冻: Prepare the jelly using the following method:

(1)将香菇、猴头菇、土瓜根、栗子内果皮、茯苓粉碎成细粉,加入其质量4倍的水,在90℃下微波提取2-3h,微波功率为15-20w,微波频率为2450 MHz; (1) Grind shiitake mushrooms, erinaceus erinaceus, soil melon root, chestnut endocarp, and poria cocos into fine powder, add water 4 times its mass, and extract by microwave at 90°C for 2-3h, with a microwave power of 15-20w, microwave The frequency is 2450 MHz;

(2)微波提取后过滤,向提取液中加入木瓜蛋白酶和纤维素酶,在55℃下酶解1h,木瓜蛋白酶用量为提取液质量的1.5‰,纤维素酶的用量为提取液质量的3.5‰,酶解以后进行冷灭酶(在室温下,250-300MPa压力下灭酶4-7分钟),过滤,取滤液备用; (2) After microwave extraction, filter, add papain and cellulase to the extract, and enzymatically hydrolyze it at 55°C for 1 hour. ‰, after enzymatic hydrolysis, cold-inactivate the enzyme (inactivate the enzyme at room temperature, under 250-300MPa pressure for 4-7 minutes), filter, and take the filtrate for later use;

(3)将草莓、芹菜切成小块,与芝麻、槐花混合,加入它们质量3倍的水榨成汁,向所得汁液中加入葡萄糖酸钙和葡萄糖酸锌,搅拌均匀,然后按照3-5%的接种量加入酵母菌、双歧杆菌和红曲霉菌的混合物(酵母菌:双歧杆菌:红曲霉菌的质量比为2.5:1:4.3),在30℃下发酵11h,发酵完后过滤,取滤液备用; (3) Cut strawberries and celery into small pieces, mix them with sesame seeds and Sophora japonica, add water three times their mass and squeeze them into juice, add calcium gluconate and zinc gluconate to the resulting juice, stir well, and then follow the 3- Add 5% of the inoculum to the mixture of yeast, bifidobacteria and monascus (the mass ratio of yeast: bifidobacteria: monascus is 2.5:1:4.3), ferment at 30°C for 11 hours, after fermentation Filter and get the filtrate for subsequent use;

(4)将玉米、海藻、鱼骨、鱼皮、鱼鳞洗净、切碎,红豆磨成粗粉,然后将它们与麦麸、红曲混合,在混合物中加入它们质量6倍的水,加热水煮,水沸腾15min后停止,混合液温度降至45℃时加入纤维素酶、果胶酶和淀粉酶(纤维素酶的用量为混合液质量的0.5‰、果胶酶的用量为混合液质量的0.8‰,淀粉酶的用量为混合液质量的1.5‰),酶解0.5h,酶解以后进行冷灭酶;然后将温度控制在40℃,向混合液中加入木瓜蛋白酶、胰蛋白酶和脂肪酶(木瓜蛋白酶的用量为混合液质量的3.5‰、胰蛋白酶的用量为混合液质量的1.8‰,脂肪酶的用量为混合液质量的0.8‰),酶解2h,酶解以后进行冷灭酶,过滤; (4) Wash and chop corn, seaweed, fish bones, fish skin, and fish scales, and grind red beans into coarse powder, then mix them with wheat bran and red yeast rice, add water 6 times their mass to the mixture, and heat Boil in water, stop after boiling for 15 minutes, add cellulase, pectinase and amylase when the temperature of the mixture drops to 45°C (the amount of cellulase is 0.5‰ of the quality of the mixture, and the amount of pectinase is 0.8‰ of the mass of amylase, and the amount of amylase is 1.5‰ of the mass of the mixed solution), enzymatic hydrolysis for 0.5h, after enzymatic hydrolysis, the enzyme is cooled; then the temperature is controlled at 40°C, and papain, trypsin and Lipase (the dosage of papain is 3.5‰ of the mass of the mixture, the dosage of trypsin is 1.8‰ of the mass of the mixture, the dosage of lipase is 0.8‰ of the mass of the mixture), enzymatic hydrolysis for 2 hours, and cooling after enzymolysis Enzyme, filter;

(5)将步骤(4)的滤液按照8%的接种量接种酵母菌,在28℃发酵3-4h,过滤取滤液备用; (5) Inoculate yeast with the filtrate of step (4) according to the inoculation amount of 8%, ferment at 28°C for 3-4 hours, filter and take the filtrate for later use;

(6)将步骤(2)、(3)、(5)的溶液混合,加入真空浓缩机中,低温真空浓缩至原体积的2/3,浓缩温度为55℃,真空度为0.095-0.1MPa; (6) Mix the solutions of steps (2), (3), and (5), add them to a vacuum concentrator, and concentrate them in a low-temperature vacuum to 2/3 of the original volume. The concentration temperature is 55°C, and the vacuum degree is 0.095-0.1MPa ;

(7)向浓缩液中加入骨胶原蛋白、透明质酸、蜂王浆、葡萄糖胺,加热至50-60℃使各成分充分溶解,然后将溶液倒入模具中,冷却后即得果冻,每粒果冻20g。 (7) Add bone collagen, hyaluronic acid, royal jelly, and glucosamine to the concentrated solution, heat to 50-60°C to fully dissolve the ingredients, then pour the solution into the mold, and get jelly after cooling. 20g.

实施例2Example 2

按照以下配方选取原料:骨胶原蛋白100份、透明质酸10份、蜂王浆20份、葡萄糖胺20份、香菇20份、猴头菇5份、土瓜根10份、栗子内果皮15份、茯苓15份、芝麻5份、草莓25份、芹菜15份、槐花5份、红曲5份、玉米15份、麦麸5份、红豆10份、海藻10份、鱼骨5份、鱼皮1份、鱼鳞5份、葡萄糖酸钙1份、葡萄糖酸锌0.3份。制备方法同实施例1。 Select raw materials according to the following formula: 100 parts of bone collagen, 10 parts of hyaluronic acid, 20 parts of royal jelly, 20 parts of glucosamine, 20 parts of shiitake mushrooms, 5 parts of Hericium erinaceus, 10 parts of soil melon root, 15 parts of chestnut endocarp, Poria cocos 15 parts, 5 parts of sesame, 25 parts of strawberry, 15 parts of celery, 5 parts of Sophora japonica, 5 parts of red yeast rice, 15 parts of corn, 5 parts of wheat bran, 10 parts of red bean, 10 parts of seaweed, 5 parts of fish bone, 1 part of fish skin 5 parts of fish scales, 1 part of calcium gluconate, and 0.3 parts of zinc gluconate. The preparation method is the same as in Example 1.

实施例3Example 3

按照以下配方选取原料:骨胶原蛋白100份、透明质酸15份、蜂王浆15份、葡萄糖胺25份、香菇15份、猴头菇10份、土瓜根5份、栗子内果皮20份、茯苓10份、芝麻10份、草莓20份、芹菜20份、槐花1份、红曲10份、玉米10份、麦麸10份、红豆5份、海藻15份、鱼骨1份、鱼皮5份、鱼鳞1份、葡萄糖酸钙5份、葡萄糖酸锌0.1份。制备方法同实施例1。 Select raw materials according to the following formula: 100 parts of bone collagen, 15 parts of hyaluronic acid, 15 parts of royal jelly, 25 parts of glucosamine, 15 parts of shiitake mushrooms, 10 parts of Hericium erinaceus, 5 parts of melon root, 20 parts of chestnut endocarp, poria cocos 10 parts, 10 parts of sesame, 20 parts of strawberry, 20 parts of celery, 1 part of sophora japonica, 10 parts of red yeast rice, 10 parts of corn, 10 parts of wheat bran, 5 parts of red bean, 15 parts of seaweed, 1 part of fish bone, 5 parts of fish skin 1 part, 1 part of fish scale, 5 parts of calcium gluconate, 0.1 part of zinc gluconate. The preparation method is the same as in Example 1.

对比例comparative example

按照以下配方选取原料:骨胶原蛋白100份、透明质酸12份、蜂王浆18份、葡萄糖胺22份、香菇16份、猴头菇6份、土瓜根6份、栗子内果皮16份、茯苓12份、芝麻6份、草莓24份、芹菜18份、槐花2份、红曲6份、玉米14份、麦麸6份、红豆6份、海藻14份、鱼骨3份、鱼皮3份、鱼鳞3份、葡萄糖酸钙2份、葡萄糖酸锌0.2份。 Select raw materials according to the following formula: 100 parts of bone collagen, 12 parts of hyaluronic acid, 18 parts of royal jelly, 22 parts of glucosamine, 16 parts of shiitake mushrooms, 6 parts of Hericium erinaceus, 6 parts of soil melon root, 16 parts of chestnut endocarp, Poria cocos 12 parts, 6 parts of sesame, 24 parts of strawberry, 18 parts of celery, 2 parts of Sophora japonica, 6 parts of red yeast rice, 14 parts of corn, 6 parts of wheat bran, 6 parts of red bean, 14 parts of seaweed, 3 parts of fish bone, 3 parts of fish skin 2 parts, 3 parts of fish scales, 2 parts of calcium gluconate, 0.2 parts of zinc gluconate.

制备方法为: The preparation method is:

(1)将香菇、猴头菇、芝麻、草莓、芹菜、槐花、红曲、海藻加水磨浆,得浆液; (1) Grinding shiitake mushrooms, Hericium erinaceus, sesame, strawberry, celery, Sophora japonica, red yeast rice, and seaweed with water to obtain slurry;

(2)将土瓜根、栗子内果皮、茯苓加水煎煮,过滤取煎煮液; (2) Add water to decoct the soil melon root, chestnut endocarp and poria cocos, filter to get the decoction;

(3)将玉米、麦麸、红豆加水磨浆,然后加热煮沸15-20min,过滤取滤液; (3) Grind the corn, wheat bran and red beans with water, then heat and boil for 15-20 minutes, and filter to get the filtrate;

(4)将鱼骨、鱼皮、鱼鳞清洗干净,真空干燥,然后粉碎成细粉; (4) Clean the fish bones, fish skin, and fish scales, dry them in vacuum, and then crush them into fine powder;

(5)将上述浆液、煎煮液、滤液,鱼骨、鱼皮、鱼鳞的细粉混合,加入骨胶原蛋白、透明质酸、蜂王浆、葡萄糖胺、葡萄糖酸钙和葡萄糖酸锌,加热至50-60℃使各成分充分溶解,然后将溶液倒入模具中,冷却后即得果冻。每粒果冻20g。 (5) Mix the above slurry, decoction, filtrate, fine powder of fish bones, fish skin and fish scales, add bone collagen, hyaluronic acid, royal jelly, glucosamine, calcium gluconate and zinc gluconate, heat to 50 -60 ℃ to fully dissolve the ingredients, then pour the solution into the mold, and after cooling, the jelly is obtained. 20g per jelly.

下面列举一部分本发明果冻的效果实验。 Below enumerate a part of the effect experiment of jelly of the present invention.

一、口感测试1. Taste test

在本市随机选取100名市民免费品尝本发明实施例1和对比例的产品,实施例1和对比例的产品原料配方一致,只是制备方法不同。其中口感得分为1-10分,1分表示口感最差,10分表示口感最好。所有尝试者均表示本发明产品酸甜爽口,无异味,而对比例产品具有一些苦、腥味,口感不是很好。具体得分情况如下: Randomly select 100 citizens in this city to taste the products of embodiment 1 of the present invention and comparative examples free of charge. The product raw material formulas of embodiment 1 and comparative examples are consistent, but the preparation method is different. The taste score ranges from 1 to 10, with 1 representing the worst taste and 10 representing the best taste. All tryers all represent that the product of the present invention is sweet and sour, and has no peculiar smell, while the comparative example product has some bitterness, fishy smell, and the mouthfeel is not very good. The specific scores are as follows:

二、美容养颜作用研究2. Research on the effect of beauty and beauty

1、在本市寻找501位女性进行自愿性实验,年龄在25-45之间,平均年龄为35.4岁。实验者具有不同的肌肤问题,经过统计,实验者具有以下肌肤问题中的至少一种,80%以上具有三种以上:1、毛孔粗糙,缺少弹性;2、皮肤干燥、缺水;3、肤色不均匀、暗沉、无光泽;4、面部有黄褐斑、雀斑等色斑;5、眼睛有细纹;6、面部有皱纹;7、皮肤有痘痘、痘印。 1. Looking for 501 women in this city to conduct voluntary sex experiments, aged between 25-45, with an average age of 35.4 years. The experimenters have different skin problems. According to statistics, the experimenters have at least one of the following skin problems, and more than 80% have three or more: 1. Rough pores and lack of elasticity; 2. Dry and dehydrated skin; 3. Skin color Uneven, dull, dull; 4. Melasma, freckles and other spots on the face; 5. Fine lines on the eyes; 6. Wrinkles on the face; 7. Acne and acne marks on the skin.

2、将以上实验者按照肌肤、年龄状态平均分为三组,各组之间无统计学差异。各组实验者分别食用实施例1、2的果冻和对比例的果冻。每天一次,每天睡前服用,每次2颗果冻。 2. The above experimenters were divided into three groups on average according to their skin and age status, and there was no statistical difference among the groups. Each group of experimenters ate the jelly of Examples 1 and 2 and the jelly of Comparative Example respectively. Once a day, before going to bed, take 2 jelly each time.

3、使用效果 3. Use effect

3.1服用一周后,各组实验者均反馈使用后皮肤光泽度增加,皮肤干燥得到很好的改善。 3.1 After taking it for a week, the experimenters in each group reported that the skin gloss increased after use, and the dryness of the skin was well improved.

3.2服用3个月后,各组实验者皮肤毛孔粗糙、色斑、痘痘、痘印、暗沉现象有了不同程度的改善,具有结果如下表1。 3.2 After taking it for 3 months, the rough skin pores, pigmentation, acne, acne marks, and dullness of the experimenters in each group have been improved to varying degrees, and the results are shown in Table 1 below.

上述显效、有效、无效判断标准为: The above criteria for judging markedly effective, effective and ineffective are:

显效:皮肤变白、变嫩,肤色变均匀,无毛孔,痘印、色斑基本消失,痘痘变小或消失; Significant effect: the skin becomes whiter and tenderer, the skin tone becomes even, without pores, acne marks and spots basically disappear, and acne becomes smaller or disappears;

有效:皮肤变白、变嫩,肤色变均匀,毛孔缩小明显,色斑、痘印淡化50%以上,痘痘缩小50%以上; Effective: the skin becomes whiter and tenderer, the skin tone becomes even, the pores shrink significantly, the stains and acne marks fade by more than 50%, and the acne shrinks by more than 50%;

无效:无以上效果。 Invalid: None of the above effects.

3.3服用1年后,各组实验者皱纹有了不同程度的改善,具体结果如下表2。 3.3 After taking it for one year, the wrinkles of the experimenters in each group have improved to varying degrees, and the specific results are shown in Table 2 below.

上述显效、有效、无效判断标准为: The above criteria for judging markedly effective, effective and ineffective are:

显效:皮肤紧致度增加,眼周轻微皱纹消失,眼袋得到明显改善,面部和眼部较深皱纹减轻50%以上; Significant effect: skin firmness increases, slight wrinkles around the eyes disappear, bags under the eyes are significantly improved, and deep wrinkles on the face and eyes are reduced by more than 50%;

有效:皮肤紧致度增加,眼周轻微皱纹消失,眼袋有所改善,面部和眼部较深皱纹减轻30%以上; Effective: Skin firmness increases, slight wrinkles around the eyes disappear, bags under the eyes are improved, and deep wrinkles on the face and eyes are reduced by more than 30%;

无效:无以上效果。 Invalid: None of the above effects.

3.4在一年服用时间内,各组实验者均为不良副作用出现,这表明本发明产品是安全的。 3.4 Within one year of taking, the experimenters in each group all had adverse side effects, which shows that the product of the present invention is safe.

三、对通便作用的研究3. Research on laxative effect

1、随机选取本市某医院收治的171名便秘患者,其中男性55例,女性116例,年龄在18-75岁之间,平均年龄45.6岁,病程2年-5年,平均2.5年。 1. Randomly select 171 constipation patients admitted to a hospital in this city, including 55 males and 116 females, aged between 18-75 years old, with an average age of 45.6 years, and a course of disease of 2-5 years, with an average of 2.5 years.

2、将患者平均分为三组,各组之间无统计学差异。各组患者分别食用实施例1、2和对比例的果冻,每天一次,每天早起空腹服用,每次2颗果冻。 2. The patients were divided into three groups on average, and there was no statistical difference among the groups. Patients in each group ate the jelly of Examples 1 and 2 and the comparative example, once a day, and took it on an empty stomach every morning, 2 jellies each time.

3、连续服用7天后,各组患者便秘程度有了不同程度的缓解,具体见表3。 3. After taking it continuously for 7 days, the degree of constipation of patients in each group has been alleviated to varying degrees, see Table 3 for details.

上述显效、有效、无效判断标准为: The above criteria for judging markedly effective, effective and ineffective are:

显效:排便次数为 2 天内排便 1 次,排便较治疗前明显通畅,短期内未复发; Markedly effective: the frequency of defecation is 1 within 2 days, and the defecation is obviously smoother than before treatment, and there is no recurrence in a short period of time;

有效:排便次数为 3 天内排便 l 次,排便较前通畅但排便时仍有一定的困难; Effective: The frequency of defecation is 1 time within 3 days, and the defecation is smoother than before, but there are still some difficulties in defecation;

无效:排便次数、性状无明显改善。 Ineffective: No obvious improvement in the frequency and character of defecation.

Claims (10)

1.一种美容养颜的果冻,其特征是包括以下重量份的原料:骨胶原蛋白100份、透明质酸10-15份、蜂王浆15-20份、葡萄糖胺20-25份、香菇15-20份、猴头菇5-10份、土瓜根5-10份、栗子内果皮15-20份、茯苓10-15份、芝麻5-10份、草莓20-25份、芹菜15-20份、槐花1-5份、红曲5-10份、玉米10-15份、麦麸5-10份、红豆5-10份、海藻10-15份、鱼骨1-5份、鱼皮1-5份、鱼鳞1-5份、葡萄糖酸钙1-5份、葡萄糖酸锌0.1-0.3份。 1. A jelly for beauty treatment, characterized in that it comprises the following raw materials in parts by weight: 100 parts of bone collagen, 10-15 parts of hyaluronic acid, 15-20 parts of royal jelly, 20-25 parts of glucosamine, 15-20 parts of shiitake mushrooms Hericium erinaceus 5-10 parts, melon root 5-10 parts, chestnut endocarp 15-20 parts, Poria cocos 10-15 parts, sesame 5-10 parts, strawberry 20-25 parts, celery 15-20 parts, Sophora japonica 1-5 parts, red yeast rice 5-10 parts, corn 10-15 parts, wheat bran 5-10 parts, red beans 5-10 parts, seaweed 10-15 parts, fish bones 1-5 parts, fish skin 1- 5 parts, fish scale 1-5 parts, calcium gluconate 1-5 parts, zinc gluconate 0.1-0.3 parts. 2.根据权利要求1所述的美容养颜的果冻,其特征是包括以下重量份的原料:骨胶原蛋白100份、透明质酸12份、蜂王浆18份、葡萄糖胺22份、香菇16份、猴头菇6份、土瓜根6份、栗子内果皮16份、茯苓12份、芝麻6份、草莓24份、芹菜18份、槐花2份、红曲6份、玉米14份、麦麸6份、红豆6份、海藻14份、鱼骨3份、鱼皮3份、鱼鳞3份、葡萄糖酸钙2份、葡萄糖酸锌0.2份。 2. The beauty jelly according to claim 1, characterized in that it comprises the following raw materials in parts by weight: 100 parts of bone collagen, 12 parts of hyaluronic acid, 18 parts of royal jelly, 22 parts of glucosamine, 16 parts of shiitake mushrooms, 6 parts of head mushroom, 6 parts of soil melon root, 16 parts of chestnut endocarp, 12 parts of poria cocos, 6 parts of sesame, 24 parts of strawberry, 18 parts of celery, 2 parts of Sophora japonica, 6 parts of red yeast rice, 14 parts of corn, 6 parts of wheat bran 6 parts of red beans, 14 parts of seaweed, 3 parts of fish bones, 3 parts of fish skin, 3 parts of fish scales, 2 parts of calcium gluconate, and 0.2 parts of zinc gluconate. 3.根据权利要求1或2所述的美容养颜的果冻,其特征是:原料中还包括调味剂。 3. The beauty jelly according to claim 1 or 2, characterized in that: the raw materials also include flavoring agents. 4.一种权利要求1或2所述的美容养颜的果冻的制备方法,其特征是包括以下步骤: 4. A method for preparing the jelly for beauty treatment according to claim 1 or 2, characterized in that it comprises the following steps: (1)将香菇、猴头菇、土瓜根、栗子内果皮、茯苓粉碎成细粉,加入其质量3-5倍的水,微波提取2-3h; (1) Grind shiitake mushrooms, Hericium erinaceus, soil melon root, chestnut endocarp, and poria cocos into fine powder, add water 3-5 times its mass, and microwave for 2-3 hours; (2)微波提取后过滤,向提取液中加入木瓜蛋白酶和纤维素酶,在50-60℃下酶解0.5-1h,酶解以后进行冷灭酶,过滤,取滤液备用; (2) After microwave extraction, filter, add papain and cellulase to the extract, enzymatically hydrolyze at 50-60°C for 0.5-1h, after enzymolysis, cold inactivate the enzyme, filter, and take the filtrate for later use; (3)将草莓、芹菜切成小块,与芝麻、槐花混合,加入它们质量3-4倍的水榨成汁,向所得汁液中加入葡萄糖酸钙和葡萄糖酸锌,搅拌均匀,然后按照3-5%的接种量加入混合菌,在28-35℃下发酵10-12h,发酵完后过滤,取滤液备用; (3) Cut strawberries and celery into small pieces, mix them with sesame seeds and Sophora japonica, add water 3-4 times their mass to squeeze out juice, add calcium gluconate and zinc gluconate to the obtained juice, stir well, and then follow Add 3-5% of the inoculum to the mixed bacteria, ferment at 28-35°C for 10-12 hours, filter after fermentation, and take the filtrate for later use; (4)将玉米、海藻、鱼骨、鱼皮、鱼鳞洗净、切碎,红豆磨成粗粉,然后将它们与麦麸、红曲混合,在混合物中加入它们质量6-8倍的水,加热水煮,水沸腾15min后停止,混合液温度降至35-50℃时加入纤维素酶、果胶酶和淀粉酶,酶解0.5-1h,酶解以后进行冷灭酶;然后将温度控制在30-45℃,向混合液中加入木瓜蛋白酶、胰蛋白酶和脂肪酶,酶解1.5-2.5h,酶解以后进行冷灭酶,过滤; (4) Wash and chop corn, seaweed, fish bones, fish skin, and fish scales, and grind red beans into coarse powder, then mix them with wheat bran and red yeast rice, and add water 6-8 times their mass in the mixture , heat and boil in water, stop after the water boils for 15 minutes, add cellulase, pectinase and amylase when the temperature of the mixture drops to 35-50°C, and enzymatically hydrolyze for 0.5-1h, and then cool the enzyme after enzymatic hydrolysis; Control at 30-45°C, add papain, trypsin and lipase to the mixed solution, enzymatic hydrolysis for 1.5-2.5h, after enzymatic hydrolysis, cold-kill the enzyme, and filter; (5)将步骤(4)的滤液按照5-8%的接种量接种酵母菌,在25-30℃发酵3-4h,过滤取滤液备用; (5) Inoculate the filtrate of step (4) with yeast according to the inoculum amount of 5-8%, ferment at 25-30°C for 3-4 hours, filter and take the filtrate for later use; (6)将步骤(2)、(3)、(5)的溶液混合,加入真空浓缩机中,低温真空浓缩至原体积的2/3; (6) Mix the solutions of steps (2), (3), and (5), add them to a vacuum concentrator, and concentrate them in vacuum at low temperature to 2/3 of the original volume; (7)向浓缩液中加入骨胶原蛋白、透明质酸、蜂王浆、葡萄糖胺,加热至50-60℃使各成分充分溶解,然后将溶液倒入模具中,冷却后即得果冻。 (7) Add bone collagen, hyaluronic acid, royal jelly, and glucosamine to the concentrated solution, heat to 50-60°C to fully dissolve the ingredients, then pour the solution into the mold, and get the jelly after cooling. 5.根据权利要求4所述的制备方法,其特征是:步骤(1)中,微波功率为15-20w,微波频率为2450 MHz,提取温度为80-100℃。 5. The preparation method according to claim 4, characterized in that: in step (1), the microwave power is 15-20w, the microwave frequency is 2450 MHz, and the extraction temperature is 80-100°C. 6.根据权利要求4所述的制备方法,其特征是:步骤(2)中,木瓜蛋白酶用量为提取液的1.5‰,纤维素酶的用量为提取液的3.5‰。 6. The preparation method according to claim 4, characterized in that: in step (2), the amount of papain is 1.5‰ of the extract, and the amount of cellulase is 3.5‰ of the extract. 7.根据权利要求4所述的制备方法,其特征是:步骤(3)中,所用混合菌为酵母菌、双歧杆菌和红曲霉菌的混合物,酵母菌:双歧杆菌:红曲霉菌的质量比为2.5:1:4.3。 7. The preparation method according to claim 4, characterized in that: in step (3), the mixed bacteria used is a mixture of yeast, bifidobacterium and monascus, yeast: bifidobacteria: monascus The mass ratio is 2.5:1:4.3. 8.根据权利要求4所述的制备方法,其特征是:步骤(4)中,纤维素酶的用量为混合液质量的0.5‰、果胶酶的用量为混合液质量的0.8‰,淀粉酶的用量为混合液质量的1.5‰;木瓜蛋白酶的用量为混合液质量的3.5‰、胰蛋白酶的用量为混合液质量的1.8‰,脂肪酶的用量为混合液质量的0.8‰。 8. The preparation method according to claim 4, characterized in that: in step (4), the dosage of cellulase is 0.5‰ of the quality of the mixture, the dosage of pectinase is 0.8‰ of the quality of the mixture, and the dosage of amylase The consumption of papain is 1.5‰ of the quality of the mixture; the consumption of papain is 3.5‰ of the quality of the mixture, the amount of trypsin is 1.8‰ of the quality of the mixture, and the consumption of lipase is 0.8‰ of the quality of the mixture. 9.根据权利要求4所述的制备方法,其特征是:步骤(6)中,浓缩温度为50-65℃,真空度为0.095-0.1MPa。 9. The preparation method according to claim 4, characterized in that: in step (6), the concentration temperature is 50-65°C, and the vacuum degree is 0.095-0.1MPa. 10.根据权利要求4所述的制备方法,其特征是:步骤(2)和(4)中,冷灭酶方法为:在室温下,250-300MPa压力下灭酶4-7分钟。 10. The preparation method according to claim 4, characterized in that: in steps (2) and (4), the method of cold inactivating the enzyme is: inactivating the enzyme at room temperature under a pressure of 250-300 MPa for 4-7 minutes.
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CN106360534A (en) * 2016-09-19 2017-02-01 蒋丽红 Skin-caring and beauty-maintaining jelly
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CN112335863B (en) * 2020-09-17 2023-04-18 山东华熙海御生物医药有限公司 Hyaluronic acid jelly and preparation method thereof

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