CN117064773A

CN117064773A - Cosmetic composition with whitening effect

Info

Publication number: CN117064773A
Application number: CN202311259779.4A
Authority: CN
Inventors: 姜伟化; 王旋; 王琳
Original assignee: Wuxi Minowa Pharmaceutical Technology Co ltd
Current assignee: Wuxi Minowa Pharmaceutical Technology Co ltd
Priority date: 2023-09-27
Filing date: 2023-09-27
Publication date: 2023-11-17

Abstract

The invention discloses a cosmetic composition with whitening effect, which comprises 0.1-2.0% of 4-butylresorcinol, 0.01-0.5% of astaxanthin, 2.0-6.0% of grease, 0.2-5.0% of antioxidant, 0.5-5.0% of emulsifier and 100% of water. The grease is caprylic acid capric acid triglyceride; the antioxidant is selected from alpha-tocopherol acetate, ascorbyl tetraisopalmitate, sodium metabisulfite and the like; the emulsifier is selected from SIMULGEL EG, SEPIGEL305, etc. The composition has good stability, no irritation and high safety, can remarkably reduce skin melanin, has the effects of whitening and removing freckle, and is very suitable for daily skin care.

Description

Cosmetic composition with whitening effect

Technical Field

The invention belongs to the field of cosmetics, and particularly relates to a cosmetic composition with a whitening effect and a preparation method thereof.

Background

With the improvement of living standard, people pay more attention to nursing and maintaining facial skin. Many skin care products and cosmetics with whitening and freckle removing effects are popular with consumers, and the products mainly realize the whitening effect by means of only physical covering effect, exfoliating, resisting oxidation, inhibiting melanosome transportation, inhibiting tyrosinase expression and the like.

4-butyl resorcinol can be directly combined with the active center area of tyrosinase, so that the tyrosinase activity in skin is effectively inhibited, meanwhile, the skin-care composition has good oxidation resistance, the oxidation reaction in the melanin synthesis process is inhibited, the formation of melanin is blocked, the generation of melanin is reduced from the source, the skin color is whitened, and color spots and chloasma are removed. Natural astaxanthin is a carotenoid which is not a source of vitamin a and has powerful antioxidant properties, and by scavenging free radicals, it significantly reduces melanin deposition, also known as "super vitamin E". With the discovery of the antioxidant capacity and other biological activities of astaxanthin, astaxanthin is gradually applied to the fields of health food, cosmetics, medical preparations and the like.

Although a large number of components with whitening effect are known, the preparation of cosmetic preparations with stable properties and meeting the actual demands still needs further research and development.

Disclosure of Invention

The invention aims to provide a cosmetic composition with whitening and freckle removing effects and a preparation method thereof. The composition has good stability, no irritation to skin, high safety, excellent whitening mechanism, and can remarkably reduce melanin content of skin, improve skin complexion grade, and has obvious freckle removing and whitening effects, and is suitable for daily skin care of people.

The invention provides the following technical scheme:

in a first aspect, the present invention provides a cosmetic composition, wherein the composition comprises, in weight percent: 0.1 to 2.0 percent of 4-butyl resorcinol, 0.01 to 0.5 percent of astaxanthin, 2.0 to 6.0 percent of grease, 0.2 to 5.0 percent of antioxidant, 0.5 to 5.0 percent of emulsifier and 100 percent of water.

Preferably, the composition comprises, in weight percent of the composition: 0.2 to 1.0 percent of 4-butyl resorcinol, 0.02 to 0.2 percent of astaxanthin, 3.0 to 6.0 percent of grease, 0.5 to 2.0 percent of antioxidant, 1.0 to 3.0 percent of emulsifier and 100 percent of water.

The oil is selected from one or more of caprylic/capric triglyceride, isopropyl palmitate, coconut oil, olive oil, soybean oil, tea seed oil and isooctyl stearate; preferably, the fat is caprylic capric triglyceride.

The antioxidant is selected from one or more of tocopherol, alpha-tocopherol acetate, tocopherol nicotinate, ascorbic acid, tetraisopalmitate ascorbate, sodium ascorbyl phosphate, magnesium ascorbyl phosphate, sodium metabisulfite, sodium bisulphite, butyl hydroxy anisole and dibutyl hydroxy toluene; preferably, the antioxidant is selected from one or more of alpha-tocopherol acetate, tetraisopalmitate ascorbate and sodium metabisulfite.

The emulsifier is one or more selected from SIMULGEL EG, SEPIGEL305, SIMUGEL INS100, SEPIPLUS 400, SIMULGEL NS; preferably, the emulsifier is selected from one or both of SIMULGEL EG and SEPIGEL 305.

The astaxanthin is a commercial astaxanthin raw material and comprises 15% of haematococcus pluvialis (HAEMATOCOCCUS PLUVIALIS) oil, 15% of glycerol, 6% of PEG-100 hydrogenated castor oil, 188% of poloxamer, 3% of sodium erythorbate and 55% of water.

In some embodiments, the cosmetic compositions of the present invention further comprise one or more of a soothing anti-sensitization agent, a skin conditioning agent, a humectant, a preservative, a pH adjusting agent.

For example, the soothing anti-allergic agent is selected from bisabolol and/or panthenol; preferably, the soothing anti-allergic agent is bisabolol. The content of the relieving and anti-allergic agent is 0.2-1.0%; preferably 0.3 to 0.8%.

For example, the skin conditioning agent is squalane and/or squalane; preferably, the soothing anti-allergic agent is squalane. The content of the skin conditioning agent is 0.5-5.0%; preferably 1.0 to 3.0%.

For example, the humectant is selected from one or more of glycerin, propylene glycol, butylene glycol, sorbitol, sodium hyaluronate, allantoin; preferably, the humectant is selected from one or more of glycerin, propylene glycol, sodium hyaluronate, allantoin. The content of the humectant is 5.0-15.0%; preferably 8.0 to 12.0%.

For example, the preservative is selected from one or more of phenoxyethanol, benzyl alcohol, hexylene glycol, pentylene glycol, parabens, p-hydroxyacetophenone, and ethylhexyl glycerol; preferably, the preservative is selected from one or more of phenoxyethanol, parabens, ethylhexyl glycerol, and the like. The content of the preservative is 0.2-2.0%; preferably 0.5 to 1.5%.

For example, the pH adjuster is an acid selected from one or more of citric acid, malic acid, tartaric acid, and gallic acid, or a base selected from tromethamine and/or triethanolamine. The pH regulator is used in an amount suitable to regulate the pH of the cosmetic composition to 4.5-7.5, preferably to 5.0-7.0.

In some embodiments, one of ordinary skill in the art may further add one or more of a emollient, thickener, chelating agent, fragrance, according to common general knowledge in the art. The specific components and amounts may be selected in accordance with common general knowledge.

In some embodiments, the cosmetic composition of the present invention comprises, in weight percent, the following components: 0.1 to 1.0 percent of 4-butyl resorcinol, 0.02 to 0.2 percent of astaxanthin, 3.0 to 6.0 percent of caprylic/capric triglyceride, 0.1 to 1.0 percent of alpha-tocopherol acetate, 0.1 to 2.0 percent of ascorbyl tetraisopalmitate, 0 to 0.005 percent of sodium metabisulfite, 0.2 to 1.0 percent of bisabolol, 0.5 to 5.0 percent of squalane, 5.0 to 12.0 percent of glycerin, 0.05 to 0.5 percent of sodium hyaluronate, 0.5 to 1.5 percent of phenoxyethanol or Antimicro9010, 1.0 to 3.0 percent of SIMULMGEL EG and water to 100 percent.

In some embodiments, the cosmetic composition of the present invention comprises, in weight percent, the following components: 0.5 to 1.0 percent of 4-butyl resorcinol, 0.05 to 0.1 percent of astaxanthin, 3.0 to 5.0 percent of caprylic/capric triglyceride, 0.3 to 0.6 percent of alpha-tocopherol acetate, 0.5 to 1.0 percent of ascorbyl tetraisopalmitate, 0.003 to 0.005 percent of sodium metabisulfite, 0.3 to 0.8 percent of bisabolol, 1.0 to 3.0 percent of squalane, 8.0 to 10.0 percent of glycerin, 0.1 to 0.15 percent of sodium hyaluronate, 0.8 to 1.2 percent of phenoxyethanol or Antimicro9010, 1.5 to 2.0 percent of SIMULMEL EG and water to 100 percent.

In one exemplary embodiment, the cosmetic composition comprises the following components in weight percent: 4-butylresorcinol 0.5%, astaxanthin 0.05%, caprylic capric triglyceride 3.0-5.0%, alpha-tocopheryl acetate 0.5%, ascorbyl tetraisopalmitate 1.0%, sodium metabisulfite 0.005%, bisabolol 0.5%, squalane 2.0%, glycerol 10.0%, sodium hyaluronate 0.1%, phenoxyethanol or Antimicro 9010.0%, SIMULGEL EG 2.0%, and water to 100%.

In a second aspect, the present invention provides a method for preparing the above cosmetic composition, comprising the steps of:

1) Preparing an aqueous phase: mixing water, emulsifier, astaxanthin, humectant, and optionally other water-soluble components (if any), and stirring;

2) Preparing an oil phase: mixing 4-butylresorcinol, oil, antioxidant, and optionally other oil-soluble components (if any), and stirring well;

3) Emulsification: mixing the oil phase with the water phase, stirring for emulsification, and optionally shearing to obtain the cosmetic composition.

The above steps may be performed at room temperature. The shearing time may be 5 to 30 minutes, for example 10 minutes.

The cosmetic composition provided by the invention is an emulsion comprising 4-butylresorcinol, astaxanthin, grease, an antioxidant, an emulsifier, water and other components, has good stability, small irritation and high safety, can obviously reduce skin melanin, improve skin complexion grade, has the effects of whitening, removing freckles, locking water and moisturizing, and is suitable for long-term skin care. The preparation method of the composition provided by the invention has simple and rapid process, adopts cold-blending emulsification, does not need heating, shortens the production time, improves the production efficiency, and is more suitable for industrial production.

Detailed Description

The invention provides a cosmetic composition containing 4-butylresorcinol and astaxanthin, which has the effects of whitening, removing freckles and protecting skin. The composition is found to have poor stability in the research process, and the problems of yellowing, transparentization, particle aggregation, layering and the like of the emulsion can occur in the storage process of different formulas. The inventor researches and screens the components of the effective components, the oil phase, the water phase, the dosage thereof and the like through repeated experiments, finally obtains the composition protected by the invention, has the advantages of good stability, low irritation and high safety, and can be used for daily whitening and skin care of people.

Unless defined otherwise, technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this invention belongs. When trade names are presented herein, it is intended to refer to their corresponding commercial products or active ingredients thereof.

In the present invention, 4-butylresorcinolThe effect of reducing melanin production is achieved by inhibiting the formation of tyrosinase and tyrosinase-related protein-1.

The terms "comprising," "including," "having," "containing," or "involving," and other variations thereof herein, are inclusive or open-ended and do not exclude additional unrecited elements or method steps.

Unless otherwise indicated, the components of the present invention are present in weight percent or parts by weight.

In the invention, the content of the effective components 4-butyl resorcinol and astaxanthin in the composition can be respectively 0.1-2.0% and 0.01-0.5%; preferably, the content thereof is 0.2 to 1.0% and 0.02 to 0.2%, respectively, for example 0.5% and 0.05%, respectively. The composition with the content range has the advantages of light pink to pink appearance, attractive and stable color, obvious whitening effect, no irritation, high safety and suitability for long-term use.

The inventor discovers that the composition prepared by adopting caprylic/capric triglyceride as grease is a pink uniform emulsion through screening oil phase components, and keeps stable character in the standing process. When mineral oil, castor oil or isopropyl myristate was used as the oil, the appearance of the resulting samples was uneven or unstable after standing, indicating that these oils were unsuitable for preparing stable and homogeneous emulsion compositions. In the present invention, the preferred oil component is caprylic capric triglyceride.

Further, the present inventors have conducted screening by using an emulsifier conventionally used for emulsion. When SIMULGEL EG or SEPIGEL305 is used as the emulsifier, the composition is prepared as a pink homogeneous emulsion with stable properties. And DEMO-90V+DECAGLYN 1-OV and DECAGLLYN 1-M+RH40、When RH40+ soybean phospholipids and Pemulen TR-1+ span 80 are used as emulsifying agents respectively, the obtained emulsion composition is obviously unstable, and demulsification, transparency, layering, pH drop or particle aggregation phenomena respectively occur in a short time, so that the emulsifying agents are not suitable for preparing the cosmetic composition. In the present invention, preferred emulsifiers are SIMULTEL EG and SEPIGEL305, preferably SIMULTEL EG.

Further, the present inventors screened different types of emulsifiers, and when SIMULGEL 600 was used as an emulsifier, the emulsion composition was discolored after standing at 40℃for 14 days, and the pink color was remarkably discolored to be white-yellow; when SIMULGEL FL is used as the emulsifier, the emulsion composition turns significantly yellow and the emulsion is slightly transparent. Therefore none of the above emulsifiers are suitable for preparing the cosmetic compositions of the present invention.

In addition, the inventors also screened antioxidants to obtain the antioxidants of the invention. While testing different formulations, no stable compositions were obtained. For example, when the formulation contains 0.2% dipotassium glycyrrhizinate, the composition can be significantly demulsified and quickly discolored to yellow when left at 40 ℃ for 7 days; when the formulation contains 0.5% of p-hydroxyacetophenone, the color of the composition becomes significantly yellow; when the formula contains 0.002% of disodium ethylenediamine tetraacetate, the composition can obviously change color and become transparent during the standing process; when 0.002% magnesium ascorbyl phosphate is included in the formulation, the composition will delaminate during the rest of the composition, resulting in unstable compositions.

Particle size D of the composition of the invention ₅₀ Is 2 to 7. Mu.m, preferably 3 to 5. Mu.m. The viscosity is 3000-6000 mPa.s. Comfortable use, easy application, and no stickiness.

The features and advantages of the cosmetic composition of the present invention will be described in further detail below with reference to specific examples. It is to be understood that the following examples are illustrative only and are not to be construed as limiting the scope of the invention. All techniques implemented based on the above description of the invention are intended to be included within the scope of the invention.

The adjuvants and reagents used in the examples below are commercially available or may be prepared by known methods. In a specific embodiment, astaxanthin is a commercially available material, available from Rui algae Biotechnology (Suzhou) Co., ltd., product number CWSE-1C, in a black red color liquid, and comprises 15% Haematococcus pluvialis (HAEMATOCOCCUS PLUVIALIS) oil, 15% glycerol, 6% PEG-100 hydrogenated castor oil, 188% poloxamer, 3% sodium erythorbate, and 55% water;

SEPIGEL305 refers to the product sold by SEPPIC corporation under the trade name SEPIGELAnd C13-14 isoparaffin (and) laurylether-7; SIMULGEL EG refers to SIMULGEL sold by SEPPIC corporation under the trade name SIMULGELSodium acrylate/sodium acryloyldimethyl taurate copolymer (and) isohexadecane (and) polysorbate-80; SIMUGEL FL refers to the product sold by SEPPIC company under the name SIMUGEL->Hydroxyethyl acrylate/sodium acryloyldimethyl taurate copolymer (and isohexadecane (and) polysorbate-60; SIMULGEL 600 refers to SIMULGEL +.>Acrylamide/sodium acryloyldimethyl taurate copolymer/isohexadecane/polysorbate-80. The preservative used in the present invention has the trade name +.>Is a compound of phenoxyethanol and ethylhexyl glycerol, and is purchased from Guangdong Dimei New Material technology Co.

The "/" in the tables of the present invention indicates that the corresponding components are not contained; or no corresponding data was measured.

The content test method of the 4-butyl resorcinol comprises the following steps: high Performance Liquid Chromatography (HPLC) method, instrument: waters Arc; chromatographic column: agilent Zorlanx-SB C18; the test conditions were as follows: column temperature: 35 ℃; flow rate: 1.0mL/min; detection wavelength: 280nm; run time: for 10min; sample injection amount: 10. Mu.L; mobile phase a phase: 0.1% formic acid-water; and B phase: acetonitrile; a diluent: 50% acetonitrile-water.

Stability investigation:

acceleration test: placing the sample in a sealed clean container, performing 6 months test at 40+ -2deg.C, sampling at 14 days, 1, 2, 3, and 6 months respectively, and comparing appearance, pH, particle size, viscosity, and content of the sample with 0 day. The sample container is an impermeable container and a glass penicillin bottle, so that the humidity condition of the test is not required. Experimental data for only a part of time points are shown in the following examples according to circumstances.

Appearance: samples were placed at different time points for visual appearance changes at 0 days and 40 ℃ ± 2 ℃ after preparation, respectively.

pH: the samples were placed in appropriate containers for use according to the direct assay in the GB/T13531 standard. Two standard buffer solutions were selected, calibrated under a temperature compensation system, the electrodes were rinsed with purified water, then blotted dry with filter paper, the electrodes carefully inserted into the sample, the electrodes immersed, and the reading recorded after the pH reading stabilized. After reading, the electrode is thoroughly cleaned for standby. The measurement result of the pH value is expressed as an average value of two measurements, and is accurate to 0.1.

Particle size: using a Markov 3000 laser diffraction particle diameter instrument, selecting a wet test, cleaning the instrument, taking 1mL of sample, putting into a dispersion tank, starting measurement after the shading degree value is stable, measuring for three times by the instrument, outputting a result, calculating an average value, recording, and selecting D ₅₀ (μm) as sample particle size data.

Viscosity: the determination was performed using an NDJ-8S series digital viscometer. The sample is placed in a suitable container for use. And a number 4 rotor is selected according to the range table, and 30 revolutions per minute is set. After the instrument is adjusted to be horizontal, the rotor is vertically inserted into the sample through the lifting knob until the liquid level is level with the middle part of the rotor groove. And starting measurement, outputting data to be output by the instrument, recording, and representing the measurement result as the average value of the two measurements. The viscosity is preferably in the range of 3000 to 6000 mPas.

Example 1 cosmetic composition

The formulation of the cosmetic composition is shown in table 1.

The preparation method comprises the following steps:

1) Preparing an oil phase: according to the formula shown in Table 1, 4-butyl resorcinol, grease (caprylic/capric triglyceride), antioxidant (alpha-tocopherol acetate, ascorbyl tetraisopalmitate), skin conditioner (squalane) and preservative (Antimicro 9010) are stirred and dissolved at room temperature, and are uniformly dispersed;

2) Preparing an aqueous phase: dispersing emulsifier (SIMULGEL EG) in all or part of water according to the formula shown in table 1, adding astaxanthin, humectant (glycerin) and optional other water-soluble components (such as sodium metabisulfite), and stirring uniformly;

3) Emulsification: adding the oil phase into the water phase, stirring for 30min, optionally adding water to the prescribed amount, and shearing for 10min to obtain the cosmetic composition in the form of emulsion.

TABLE 1 formulation of cosmetic compositions

The experimental results show that the emulsion compositions of the formulas 1 to 3 are respectively placed at 40 ℃ for 6 months, have no obvious change in appearance, are pink uniform emulsion, have no granular feel and layering phenomenon, and have no obvious change in particle size. The pH of the composition remained stable and did not change significantly. The viscosity is basically kept stable without obvious change. The emulsion compositions of formulas 1-3 have good stability.

Example 2 cosmetic composition

The formulation of the cosmetic composition is shown in table 2.

The preparation method comprises the following steps: reference is made to example 1.

TABLE 2 formulation of cosmetic compositions

The experimental results show that the emulsion composition of the formula 4-the formula 6 has no obvious change in appearance after being placed for 6 months at 40 ℃, is light pink uniform emulsion, has no granular feel and layering phenomenon, and has no obvious change in particle size. The pH of the composition remained stable without significant changes. The viscosity is basically kept stable without obvious change. The content of 4-butyl resorcinol is stable. The emulsion compositions of formulas 4-6 have good stability.

Example 3 cosmetic composition

The formulation of the cosmetic composition is shown in table 3.

TABLE 3 formulation of cosmetic compositions

The experimental results show that the emulsion composition of the formula 7-the formula 9 has no change in appearance after being placed for 6 months at 40 ℃, is light pink uniform emulsion, has no granular feel and layering phenomenon, and has no obvious change in particle size. The pH of the composition remained stable and did not change significantly. The viscosity is kept stable without obvious change. The content of 4-butyl resorcinol is stable. The emulsion compositions of formulas 7-9 have good stability.

Comparative example 1 cosmetic composition

Formulations 10 to 12 of the cosmetic compositions are shown in Table 4.

TABLE 4 formulation of cosmetic compositions

The compositions of formulas 10 to 12 respectively adopt mineral oil, castor oil and isopropyl myristate which are conventionally used in emulsion as grease, and experimental results show that the appearance of the compositions is obviously changed, the properties are uneven or unstable after the compositions are placed at normal temperature of 25 ℃ or 40 ℃ for a short time, so that the grease is not suitable for being used in the cosmetic composition of the invention. The preferred oil component of the present invention is caprylic capric triglyceride.

Comparative example 2 cosmetic composition

Formulations 13 to 16 of the cosmetic compositions are shown in Table 5.

TABLE 5 formulation of cosmetic compositions

The compositions of formulas 16-18 respectively adopt DEMO-90V+DECAGLYN 1-OV and DECAGLYN 1-M +

RH40、As the result of experiments, RH40+ soybean phosphatide and Pemulen TR-1+ span 80 are used as emulsifying agents, and the obtained emulsion composition is obviously unstable and has the phenomena of demulsification, transparency, layering, pH drop or particle aggregation respectively in a short time. Therefore, these emulsifiers are not suitable for use in the cosmetic compositions of the present invention.

Comparative example 3 cosmetic composition

Formulations 17 to 19 of the cosmetic compositions are shown in Table 6.

TABLE 6 formulation of cosmetic compositions

Formulas 17-19 further examined the effect of different emulsifier types on cosmetic compositions. The experimental result shows that when SIMULGEL 600 is used as an emulsifier, the emulsion composition changes color after being placed for 14 days at 40 ℃, the pink color obviously changes into white yellow; when SIMULGEL FL is used as the emulsifier, the emulsion composition turns significantly yellow and the emulsion is slightly transparent; when SEPIGEL305 was used as the emulsifier, the emulsion composition turned slightly yellow. Formulations 17-19 remained stable for a relatively long period of time at ambient conditions (data not shown), but were less stable at 40 ℃. In the present invention, the emulsifier SEPIGEL305 is an optional emulsifier, but a preferred emulsifier is simullel EG.

Test example 1 skin patch test

The cosmetic composition of the present invention was subjected to a human skin patch test for safety and irritation. The test is entrusted to a third party analysis and detection organization (Shanghai micro-spectrum detection technology group).

1. Materials and methods

1. Test article: cosmetic composition formulation 8

2. Negative control: blank + filter control

3. The subject: 30 persons total, male 1 person, female 29 person, age 26-60 years, average age 47.57+ -8.75 years, meet the volunteer selection criteria of the subjects.

4. Plaque test method: selecting qualified plaque test equipment, placing 0.020-0.025 ml of test object into plaque test equipment by a closed type banon test method, applying a hyposensitization tape to the forearm curved side of a subject, removing the test object 24 hours later, observing skin reaction respectively 0.5, 24 and 48 hours later, and recording the result according to the skin reaction grading standard in cosmetic safety technical Specification (2015).

2. Test results

The skin response grading criteria for the skin closed patch test are shown in Table 7. The results of the human skin patch test are shown in Table 8.

TABLE 7 skin response grading Standard for skin closed Patch test

TABLE 8 human skin patch test results

The test results of the human skin patch show that all subjects do not have skin adverse reactions, so that the cosmetic composition provided by the invention has no irritation to skin, is high in safety, and is suitable for daily skin care.

Test example 2 whitening and freckle-removing efficacy test of cosmetic composition

The present test example conducted a whitening and freckle-removing efficacy test on the cosmetic composition of the present invention.

1. Materials and methods

1. Test article: cosmetic composition of inventive formulation 8

2. Negative control: blank control

3. Positive control: 7% of ascorbic acid (vitamin C) products are prepared according to the formula of annex I of a cosmetic freckle removing and whitening efficacy test method-a first method ultraviolet-induced human skin blackening model freckle removing and whitening efficacy test method in cosmetic safety technical Specification (2015 edition).

4. The subject: 30 people total, 10 men, 20 women, 18-54 years of age, and average age of 36.10+/-12.41 years, and meet the volunteer selection standard of the subjects.

5. The test method comprises the following steps: the test was carried out according to the specific requirements of cosmetic safety Specification (2015 edition). Selecting the back of the subject as the test area not smaller than 0.5cm ² And should be located within each of the application areas. MED of the test site of the subject is determined. The same irradiation spot was irradiated with a day 1 with 0.75 times MED dose for 4 consecutive days using a solar simulator. The 4 days after the end of irradiation are skin darkening periods without any treatment. On the 5 th day after the irradiation, the skin of each test area is smeared with corresponding test objects according to a random table, and the test objects are connectedThe subjects were continuously applied for at least 4 weeks, and the skin color was visually evaluated and instrumentally detected at 1 week, 2 weeks, 3 weeks and 4 weeks after the application, and the ITA value (representing the darkness of skin color), MI value (representing the melanin content of skin) and visual skin color grade improvement degree were detected, respectively, and were statistically analyzed, wherein one item having significance compared with the negative control group was considered to have a whitening effect.

2. Test results

Statistical analysis results of ITA DEG and MI at each time point before and after the use of each group of samples are shown in Table 9; the results of the statistical analysis of the improvement degree of the visual skin tone grade are shown in Table 10.

TABLE 9 statistical analysis results of ITA℃and MI at various time points before and after use

Note that: compared with the negative control group, the MI value regression coefficient P of the formula 8 is less than 0.05, and has significant difference.

TABLE 10 statistical analysis of visual skin tone grade improvement levels at various time points before and after use

Note that: compared with a negative control group, the visual skin color grade regression coefficient P of the formula 8 is less than 0.05, and has a significant difference.

The experimental result shows that the product of the formula 8 has an effect of improving the skin depth; the improvement degree of the MI value of the skin is obviously better than that of a negative control group in weeks 2, 3 and 4, and has obvious difference, so that the composition of the invention can obviously reduce the melanin content in the skin; the degree of improvement in the visual complexion grade of the skin was significantly better at weeks 3 and 4 than the negative control group, indicating that the composition of the invention can significantly improve the skin complexion. In summary, the composition of the invention has remarkable whitening effect.

The inventor performs extensive experimental study on various components and proportions in the composition formula, and performs screening, testing and comparison through a large number of experiments to obtain the cosmetic composition with the whitening and freckle removing effects. The composition can be placed for 6 months at high temperature, does not generate obvious color change, does not generate adverse phenomena such as demulsification, layering, particle aggregation and the like, can keep long-term stability of the cosmetic composition at normal temperature of 25 ℃, and has very good stability. In addition, the human body experiment result shows that the cosmetic composition formula of the invention does not generate irritation to skin after being used, has good safety, can obviously reduce the black content of the skin, obviously improve the skin complexion grade, and has obvious freckle removing and whitening effects.

The above examples and test examples are merely illustrative of the features and advantages that the cosmetic compositions of the present invention possess. Various modifications and substitutions of the described embodiments will occur to those skilled in the art without the need for inventive faculty in view of the present disclosure, and are intended to be included within the scope of the following claims without departing from the spirit and principles of the invention.

Claims

1. A cosmetic composition with whitening effect comprises the following components in percentage by weight: 0.1 to 2.0 percent of 4-butyl resorcinol, 0.01 to 0.5 percent of astaxanthin, 2.0 to 6.0 percent of grease, 0.2 to 5.0 percent of antioxidant, 0.5 to 5.0 percent of emulsifier and 100 percent of water.

2. The cosmetic composition of claim 1, wherein the oil is selected from one or more of caprylic capric triglyceride, isopropyl palmitate, coconut oil, olive oil, soybean oil, tea seed oil, isooctyl stearate; the antioxidant is selected from one or more of tocopherol, alpha-tocopherol acetate, tocopherol nicotinate, ascorbic acid, tetraisopalmitate ascorbate, sodium ascorbyl phosphate, magnesium ascorbyl phosphate, sodium metabisulfite, sodium bisulphite, butyl hydroxy anisole and dibutyl hydroxy toluene; the emulsifier is selected from one or more of SIMULGEL EG, SEPIGEL305, SIMUGEL INS100, SEPIPLUS 400, SIMULGEL NS.

3. The cosmetic composition of claim 1, comprising the following components in weight percent: 0.2 to 1.0 percent of 4-butyl resorcinol, 0.02 to 0.2 percent of astaxanthin, 3.0 to 6.0 percent of grease, 0.5 to 2.0 percent of antioxidant, 1.0 to 3.0 percent of emulsifier and 100 percent of water.

4. A cosmetic composition according to any one of claims 1 to 3 wherein the oil is caprylic capric triglyceride; the antioxidant is selected from one or more of alpha-tocopherol acetate, ascorbyl tetraisopalmitate and sodium metabisulfite; the emulsifier is selected from one or both of SIMULGEL EG and SEPIGEL 305.

5. The cosmetic composition of any one of claims 1-4, further comprising one or more of a soothing anti-sensitization agent, a skin conditioning agent, a moisturizing agent, a preservative, a pH adjuster.

6. The cosmetic composition of claim 5, wherein the soothing anti-allergic agent is selected from the group consisting of bisabolol and panthenol; the skin conditioning agent is selected from squalane and squalane; the humectant is one or more selected from glycerol, propylene glycol, butanediol, sorbitol, sodium hyaluronate and allantoin; the preservative is one or more selected from phenoxyethanol, benzyl alcohol, hexanediol, pentanediol, p-hydroxybenzoates and p-hydroxyacetophenone; the pH regulator is acid or alkali.

7. The cosmetic composition of claim 6, wherein the cosmetic composition comprises, in weight percent: 0.2 to 1.0 percent of release anti-allergic agent, 0.5 to 5.0 percent of skin conditioning agent, 5.0 to 15.0 percent of humectant and 0.2 to 2.0 percent of preservative;

preferably, the cosmetic composition comprises: 0.3 to 0.8 percent of release anti-allergic agent, 1.0 to 3.0 percent of skin conditioning agent, 8.0 to 12.0 percent of humectant and 0.5 to 1.5 percent of preservative.

8. The cosmetic composition according to any one of claims 1 to 7, comprising, in weight percent: 0.2 to 1.0 percent of 4-butyl resorcinol, 0.02 to 0.2 percent of astaxanthin, 3.0 to 6.0 percent of caprylic/capric triglyceride, 0.1 to 1.0 percent of alpha-tocopherol acetate, 0.1 to 2.0 percent of ascorbyl tetraisopalmitate, 0 to 0.005 percent of sodium metabisulfite, 0.2 to 1.0 percent of bisabolol, 0.5 to 5.0 percent of squalane, 5.0 to 12.0 percent of glycerin, 0.05 to 0.5 percent of sodium hyaluronate, 0.5 to 1.5 percent of phenoxyethanol or Antimicro9010, 1.0 to 3.0 percent of SIMULMEL EG and water to 100 percent;

preferably, the cosmetic composition comprises the following components in weight percent: 0.5 to 1.0 percent of 4-butyl resorcinol, 0.05 to 0.1 percent of astaxanthin, 3.0 to 5.0 percent of caprylic/capric triglyceride, 0.3 to 0.6 percent of alpha-tocopherol acetate, 0.5 to 1.0 percent of ascorbyl tetraisopalmitate, 0.003 to 0.005 percent of sodium metabisulfite, 0.3 to 0.8 percent of bisabolol, 1.0 to 3.0 percent of squalane, 8.0 to 10.0 percent of glycerin, 0.1 to 0.15 percent of sodium hyaluronate, 0.8 to 1.2 percent of phenoxyethanol or Antimicro9010, 1.5 to 2.0 percent of SIMULMEL EG and water to 100 percent;

preferably, the cosmetic composition comprises the following components in weight percent: 4-butylresorcinol 0.5%, astaxanthin 0.05%, caprylic capric triglyceride 3.0-5.0%, alpha-tocopheryl acetate 0.5%, ascorbyl tetraisopalmitate 1.0%, sodium metabisulfite 0.005%, bisabolol 0.5%, squalane 2.0%, glycerol 10.0%, sodium hyaluronate 0.1%, phenoxyethanol or Antimicro 9010.0%, SIMULGEL EG 2.0%, and water to 100%.

9. The cosmetic composition of any one of claims 1 to 8, wherein the astaxanthin comprises 15% haematococcus pluvialis (HAEMATOCOCCUS PLUVIALIS) oil, 15% glycerol, 6% PEG-100 hydrogenated castor oil, 188% poloxamer, 3% sodium erythorbate, 55% water.

10. A method of preparing the cosmetic composition of any one of claims 1 to 9, comprising the steps of:

1) Preparing an aqueous phase: mixing water, emulsifier, astaxanthin, humectant and optionally other water-soluble components, and stirring uniformly;

2) Preparing an oil phase: mixing 4-butyl resorcinol, grease, antioxidant and optionally other oil-soluble components, and uniformly stirring;

3) Emulsification: mixing the oil phase with the water phase, stirring, emulsifying, and shearing to obtain the cosmetic composition.

Preferably, the above steps are performed at room temperature.