KR101917774B1 - A preservative composition of cosmetics comprising Nigella sativa seed extract as an active ingredient - Google Patents

Abstract

The present invention relates to a composition for preserving cosmetic products containing a black cumin seed extract as an active ingredient. The composition for preserving the cosmetic products is excellent in antibacterial and antifungal effects as compared with conventionally used chemical preservatives, and also shows outstanding preservative effects when processed as cosmetic ingredients, without harmfulness to human body such as skin irritation, thereby being substituted for a chemical preservative and useful for cosmetic compositions and compositions for preserving cosmetic products.

Description

[0001] The present invention relates to a preservative composition for preserving cosmetics containing an extract of blackcumin, as an active ingredient,

To a cosmetic preserving composition containing an extract of blackcumin seed as an active ingredient.

Many products, such as foods, medicines, and cosmetics, generally contain a chemical preservative or preservative to prevent decay by microorganisms or bacteria and to kill microorganisms over time in order to maintain quality for a long time. Examples of such chemical preservatives or preservatives include parabens, imidazolidinyl urea, phenoxyethanol, paraoxyanionic acid esters, benzalkonium chloride, benzoic acid, sodium benzoate, methyl paraoxybenzoate, ethyl paraoxybenzoate, Sorbic acid, potassium sorbate, sodium sorbate, chlorobutanol, benzethonium chloride, phenol, cresol, chlorocresol and the like are generally used.

Particularly, it is essential to use preservatives or preservatives to prevent deterioration in cosmetics or medicines and to suppress the propagation of bacteria and microorganisms, to preserve them during the distribution period and to maintain purity, and it is necessary to use preservatives or preservatives in the cosmetics or medicines Even safer parabens preservatives have been shown to be effective against skin allergies (Andrea Counti et al., Contact Dermatitis, 1997, 37; 35-36) and the potential for environmental hormones (Edwin et al., Toxicology and Applied Pharmacology, 1998, . In addition, food preservatives are also disallowed within acceptable standards and new problems such as rapid and chronic toxic mutagenesis due to persistent accumulation in the body are emerging (Shin Dong-hwa, Food Science and Technology, 1990, 23 (4) 68 -72).

As described above, parabens are widely used as preservatives for food and cosmetics, which inhibit the growth of microorganisms even at a concentration that does not harm human body. Parabens are widely used as preservatives for foodstuffs and cosmetics, and include parahydroxybenzoate, butyl parahydroxybenzoate, Isobutyl oxybenzoate, isopropyl p-oxybenzoate and propyl paraoxybenzoic acid, and various parabens such as methyl paraben, ethyl paraben, isobutyl paraben, isopropyl paraben, and propyl paraben are used as the abbreviation.

As described above, since the harmfulness of parabens has been known, consumers have been reluctant to use parabens-containing cosmetics. Accordingly, some cosmetics companies have introduced paraben-free products, but also use other kinds of chemical preservatives that replace parabens In fact. In addition, recently, the kinds of preservatives that can be used in medicines or cosmetics are gradually decreasing, and the amount of preservatives used is also decreasing.

It is necessary to develop a natural preservative that can replace chemical preservatives due to the above-mentioned problems. However, it is necessary to use a large amount of natural preservatives due to the dark color, odor, antimicrobial activity, I can not do that.

Black Cumin Seed is called black seed or black seed of Nigella sativa. It is a plant belonging to the family Ranunculaceae. Origin is a unique plant that has been used as herbal medicine for over 2,000 years in the Middle East, Southwest Asia or Southeast Asia. It is also called the "covenant to repair all diseases except death". In fact, there are many different types of drugs such as Thymoquinone, Thymohydroquinone, Nigellimine-N-oxide, Nigellicine, Nigellidine, Nigellone, Dithymoquinone, Thymol, Arvacrol, 6-methoxycoumarin, 7-hydroxy- coumarin, Oxy-coumarin, Alpha-hedrin, Sterylglucoside, fatty acids, Essential amino acids, Ascorbic acid, Iron and calcium.

Among these, the thymoquinone component exerts a remarkable effect in the treatment of fungi, and is also used to treat athlete's foot, fungus, and mold vaginitis. In addition, it is known that it has the effect of alleviating psoriasis and acne. In the field of medicine, it is known to help improve immune diseases, asthma and allergy. It is also used for skin diseases such as inflammation and eczema.

One aspect of the present invention is to provide a composition for preserving cosmetics comprising a blackcumin seed extract as an active ingredient.

Another aspect of the present invention is to provide a cosmetic composition comprising the preservative composition.

According to one aspect of the present invention,

Glyceryl mono fatty acid esters; At least one polyhydric alcohol selected from the group consisting of 1,2-hexanediol, pentylene glycol, 1,2-octanediol, ethylhexyl glycerin, 1,3-butyleneglycol and glycerin; And a Nigella sativa seed extract, which is a composition for preserving cosmetics,

The glyceryl mono fatty acid ester is contained in the composition in an amount of 30 to 85 parts by weight, the polyhydric alcohol is contained in 15 to 70 parts by weight,

Wherein the blackcumin seed extract is contained in an amount of 0.01 to 1 part by weight based on 100 parts by weight of the total of the glyceryl mono fatty acid ester and the polyhydric alcohol.

According to another aspect of the present invention,

Glyceryl mono fatty acid esters; At least one polyhydric alcohol selected from the group consisting of 1,2-hexanediol, pentylene glycol, 1,2-octanediol, ethylhexyl glycerin, 1,3-butyleneglycol and glycerin; And a Nigella sativa seed extract, which is a composition for preserving cosmetics,

The glyceryl mono fatty acid ester is contained in the composition in an amount of 30 to 85 parts by weight, the polyhydric alcohol is contained in 15 to 70 parts by weight,

The cosmetic composition comprising 0.01 to 5 parts by weight of a composition for preserving cosmetics, wherein the blackcumin seed extract is contained in an amount of 0.01 to 1 part by weight based on 100 parts by weight of the total of the glyceryl mono fatty acid ester and the polyhydric alcohol.

The composition for preserving cosmetics containing a blackcumin seed extract as an active ingredient is excellent in antibacterial and antifungal effects compared to conventional chemical preservatives and has excellent antiseptic effect when it is prepared into a cosmetic, It is not harmful to humans and can be usefully used in cosmetic preservation compositions and cosmetic compositions, and chemical preservatives can be substituted.

BRIEF DESCRIPTION OF THE DRAWINGS Fig. 1 is an image showing the result of evaluating the flushing force of a lotion prepared using a composition for preserving cosmetics containing an extract of blackcumin seed as an active ingredient. Fig.
Fig. 2 is an image showing the result of evaluating the antifouling force of a toner prepared by using a composition for preserving cosmetics containing a blackcumin seed extract as an active ingredient. Fig.

Hereinafter, the present invention will be described in detail.

The term " antibacterial " as used in the present invention means to kill or inhibit or inhibit microorganisms harmful to human body such as bacteria or fungi. The antimicrobial power leads to a preservative effect that prevents corruption from microorganisms.

It is an object of the present invention to provide a preservative composition containing a natural substance capable of replacing a conventional chemical preservative or preservative, and in particular, to provide a composition for preserving cosmetics applicable to cosmetics.

In order to accomplish this, one aspect of the present invention provides a composition for preserving cosmetics comprising a blackcumin seed extract as an active ingredient.

Specifically,

Glyceryl mono fatty acid esters; At least one polyhydric alcohol selected from the group consisting of 1,2-hexanediol, pentylene glycol, 1,2-octanediol, ethylhexyl glycerin, 1,3-butyleneglycol and glycerin; And a Nigella sativa seed extract, which is a composition for preserving cosmetics,

The glyceryl mono fatty acid ester is contained in the composition in an amount of 30 to 85 parts by weight, the polyhydric alcohol is contained in 15 to 70 parts by weight,

Wherein the blackcumin seed extract is contained in an amount of 0.01 to 1 part by weight based on 100 parts by weight of the total of the glyceryl mono fatty acid ester and the polyhydric alcohol.

When the glyceryl mono fatty acid ester is contained in an amount of less than 30 parts by weight, the synergistic effect may be lowered and the preservative effect may be lowered. When the glyceryl mono fatty acid ester is included in an amount exceeding 85 parts by weight, There is a problem in usage.

If the polyhydric alcohol is contained in an amount of less than 15 parts by weight, there may be a problem of poor stability in use, a poor synergistic effect of preservation, and a problem of less than synergistic effect of preservation. When the polyhydric alcohol is contained in an amount exceeding 70 parts by weight, There may be a problem that the preservation synergy effect is lowered.

When the blackcumin seed extract is contained in an amount of less than 0.01 based on 100 parts by weight of the total amount of the glyceryl mono fatty acid ester and the polyhydric alcohol in the composition, there may be a problem that the preservation synergy effect is lowered, and when the content is more than 1 part by weight, There may be a problem causing skin irritation.

In the composition for preserving cosmetics, glyceryl mono fatty acid ester is a naturally occurring ingredient and has no irritation to the skin, helps to manage smoothly and flexibly, has antibacterial effect, and is added for synergy with blackcumin seed extract do. However, it has a disadvantage that phase stability is poor when it is contained in a large amount.

The polyhydric alcohol is added to enhance the phase stability of the blackcumin seed extract and the glyceryl mono fatty acid ester.

In terms of improving the preservation effect of the composition of the present invention,

The glyceryl mono fatty acid ester may be included in the composition in an amount of 45 to 65 parts by weight, the polyhydric alcohol may be included in 35 to 55 parts by weight,

The glyceryl mono fatty acid ester may be contained in the composition in an amount of 50 to 60 parts by weight, and the polyhydric alcohol may be contained in 40 to 50 parts by weight.

The blackcumin seed extract may be included in the composition in an amount of 0.1 to 1 part by weight based on 100 parts by weight of the total of the glyceryl mono fatty acid ester and the polyhydric alcohol, and may be included in an amount of 0.1 to 0.5 part by weight.

In the present invention, the polyhydric alcohol may be a combination of 1,2-hexanediol and ethylhexyl glycerin.

The glyceryl mono fatty acid ester may be contained in the composition in an amount of 30 to 85 parts by weight, the 1,2-hexanediol may be contained in the range of 14 to 55 parts by weight, the ethylhexyl glycerin may be included in the range of 1 to 15 parts by weight,

The composition may include 45 to 65 parts by weight of glyceryl mono fatty acid ester, 30 to 50 parts by weight of 1,2-hexanediol, 5 parts by weight of ethylhexyl glycerin,

The glyceryl mono fatty acid ester may be contained in the composition in an amount of 50 to 60 parts by weight, the 1,2-hexanediol may be contained in 35 to 45 parts by weight, and the ethylhexyl glycerin may be included in 5 parts by weight.

The glyceryl mono fatty acid ester is not particularly limited but may be one containing a C _8-18 glycerin derivative and may be glyceryl monocaprylate (C8), glyceryl monolaurate (C12), glyceryl monomyrin (C14), glyceryl monopalmitate (C16), glyceryl monostearate (C18), and glyceryl monooleate (C18: 1).

The blackcumin seed extract may be extracted according to a conventional extraction method, or a commercial product may be used. In the present invention, any one selected from the group consisting of C _1-4 lower alcohol and water or a combination thereof is obtained as an extraction solvent, but the present invention is not limited thereto. The solvent of the prepared blackcumin seed extract can be removed by filtration and concentration. The filtration may be performed under reduced pressure using a filter paper, and the concentration may be carried out by using a vacuum concentrator such as a rotary evaporator.

The cosmetic may be any one selected from the group consisting of lotion, toner, skin, lotion, emulsion, cream, essence, serum, gel, and pack; Make-up cosmetics selected from lipsticks, tints, lip gloss, lip balm, viscose cream, foundation, powder, mascara, eye shadow and eyeliner; Skin exfoliation scrub, cleansing water, cleansing oil, cleansing cream and foam cleansing; Hair cosmetics, hair cosmetics, hair shampoos, rinse, and treatments; And body cosmetics selected from the group consisting of body scrubs, body lotions, body creams, and body creams, but the present invention is not limited thereto.

In particular, the composition for preserving cosmetics of the present invention is a preservative composition containing a natural substance capable of replacing a conventional chemical preservative, and exhibits a similar or remarkably excellent effect as a chemical preservative by using a blackcumin seed extract (Experiment See Example 1), and can be used as a preservative composition which can replace a chemical preservative.

On the other hand, in the composition for preserving cosmetics of the present invention, when the blackcumin seed extract is used alone, strong skin irritation is induced and it can not be used alone (see Experimental Example 2).

However, the composition for preserving cosmetics of the present invention is excellent in anti-bacterial and antifungal effect by mixing glyceryl mono fatty acid ester, 1,2hexanediol and ethylhexyl glycerin in a specific composition ratio, (See Experimental Example 2), and can be usefully used as a composition for preserving cosmetics.

The composition exhibits an antibacterial or antifungal effect. In this case, the bacterium may be at least one selected from the group consisting of Escherichia coli and Staphlyococcus aureus, and the fungus may be selected from the group consisting of Candida albicans and Aspergillus niger . However, the bacterium and fungus are only examples, and the antibacterial effect exhibited by the composition is not limited to the bacteria.

The composition for preserving cosmetics may be prepared by mixing each composition.

Another aspect of the present invention provides a cosmetic composition comprising the composition for preserving cosmetics.

Specifically, it is a composition for preserving cosmetics comprising glyceryl mono fatty acid ester, 1,2hexanediol, ethylhexyl glycerin and Nigella sativa seed extract,

The glyceryl mono fatty acid ester is contained in 30 to 80 parts by weight, the 1,2-hexanediol is contained in 15 to 55 parts by weight, the ethylhexyl glycerin is contained in 1 to 15 parts by weight,

Wherein the blackcumin seed extract is contained in an amount of 0.01 to 1 part by weight based on 100 parts by weight of the total of the glyceryl mono fatty acid ester, 1,2 hexanediol and ethylhexyl glycerin, and 0.01 to 5 parts by weight of the composition for preserving cosmetics / RTI >

Wherein the cosmetic composition is any cosmetic composition selected from the group consisting of lotion, toner, skin, lotion, emulsion, cream, essence, serum, gel, and pack; Make-up cosmetics selected from lipsticks, tints, lip gloss, lip balm, viscose cream, foundation, powder, mascara, eye shadow and eyeliner; Skin exfoliation scrub, cleansing water, cleansing oil, cleansing cream and foam cleansing; Cosmetics for hair, which is selected from the group consisting of shampoos, rinses and treatments; And a cosmetic for body which is any one selected from the group consisting of body scrubs, body lotions, body creams, and body creams, but is not limited thereto.

The cosmetic composition of the present invention may further contain, in addition to the compound represented by the formula (1) of the present invention or a pharmaceutically acceptable salt thereof, a fatty substance, an organic solvent, a solubilizing agent, a thickening agent and a gelling agent, a softening agent, A foaming agent, a fragrance, a surfactant, water, an ionic or nonionic emulsifier, a filler, a sequestering and chelating agent, a preservative, a vitamin, a barrier, a wetting agent, A lipophilic active agent, a lipid vesicle, or any other ingredient conventionally used in compositions for improving skin aesthetics.

In addition, the components can be introduced in amounts commonly used in the dermatology field.

Furthermore, the cosmetic composition according to the present invention can be used as a cosmetic composition in the form of a solution, an external ointment, a cream, a foam, a nutritional lotion, a softening water, a pack, a soft water, But are not limited to, formulations such as suspensions, emulsions, pastes, gels, lotions, powders, soaps, surfactant-containing cleansing, oils, powder foundations, emulsion foundations, wax foundations, patches or sprays .

In addition, the cosmetic composition of the present invention may further comprise at least one cosmetically acceptable carrier incorporated in a cosmetic composition for general skin, and examples thereof include oil, water, a surfactant, a moisturizer, a lower alcohol, A thickening agent, a chelating agent, a coloring matter, an antiseptic, a perfume, and the like may be appropriately compounded, but the present invention is not limited thereto.

The cosmetically acceptable carrier to be contained in the cosmetic composition of the present invention varies depending on the formulations. When the formulation of the present invention is an ointment, a paste, a cream or a gel, the carrier component may be an animal oil, a vegetable oil, a wax, a paraffin, a starch, a tracer, a cellulose derivative, polyethylene glycol, silicone, bentonite, silica, talc, zinc oxide Mixtures of these may be used.

When the formulation of the present invention is a powder or a spray, lactose, talc, silica, aluminum hydroxide, calcium silicate, polyamide powder or a mixture thereof may be used as the carrier component, Propellants such as fluorohydrocarbons, propane / butane or dimethyl ether.

When the formulation of the present invention is a solution or an emulsion, a solvent, a solubilizing agent or an emulsifying agent is used as a carrier component, and examples thereof include water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl benzoate, -Butyl glycol oil, and in particular fatty acid esters of cottonseed oil, peanut oil, corn oil, olive oil, castor oil and sesame oil, glycerol aliphatic ester, polyethylene glycol or sorbitan can be used.

When the formulation of the present invention is a suspension, a carrier such as water, a liquid diluent such as ethanol or propylene glycol, a suspension such as ethoxylated isostearyl alcohol, polyoxyethylene sorbitol ester and polyoxyethylene sorbitan ester, Crystalline cellulose, aluminum metahydroxide, bentonite, agar or tracant, etc. may be used.

When the formulation of the present invention is a soap, alkali metal salts of fatty acids, fatty acid hemiesters, fatty acid protein hydrolizates, isethionates, lanolin derivatives, aliphatic alcohols, vegetable oils, glycerol, sugars and the like are used as carrier components .

Hereinafter, the present invention will be described in detail with reference to Examples and Experimental Examples.

However, the following Examples and Experimental Examples are merely illustrative of the present invention, and the present invention is not limited to the following Examples and Experimental Examples.

Preparation Example 1 Preparation of blackcumin seed extract

Blackcumin seed pulverized water and an aqueous ethanol solution containing 0 to 90% (v / v) ethanol are put into a reflux extractor and heated. The extract was filtered, and the obtained extract was concentrated under reduced pressure using a rotary evaporator to obtain a blackcumin seed extract.

≪ Example A > Preparation of a composition for preserving cosmetics containing blackcumin seed extract as an active ingredient

The cosmetic preserving compositions of Examples A-1 to A-7 were prepared by mixing the blackcumin seed extract, glyceryl monocaprylate, 1,2-hexanediol and ethylhexyl glycerin obtained in Preparation Example 1 above. The composition ratios are shown in Table 1 below. The criteria for the compositional ratios below are parts by weight.

Material name Example
A-1 Example
A-2 Example
A-3 Example
A-4 Example
A-5 Example
A-6 Example
A-7 Black Cumin seed extract 0.5 0.3 0.1 0.3 0.3 0.3 0.3 Glyceryl monocaprylate 70.0 65.0 65.0 60.0 55.0 50.0 45.0 1,2-hexanediol 25.0 30.0 30.0 35.0 40.0 45.0 50.0 Ethylhexyl glycerin 5.0 5.0 5.0 5.0 5.0 5.0 5.0

≪ Example B > Production of Lotion

Lotions were prepared using a composition for preserving cosmetics containing the blackcumin seed extract of the present invention as an active ingredient. Specifically, the lotions of Examples B-1 to B-3 were prepared using the composition prepared in Example A, and the composition ratios thereof are shown in Table 2 below. The lotion prepared in Example A-4 and the lotion prepared in Example A-5 were used in Example B-2 and Example A-6. Example B-3. The criteria for the compositional ratios below are parts by weight.

No Material name content (%) One Water 78.69 2 Glycerin 4.00 3 Butylene Glycol 3.00 4 Disodium EDTA 0.02 5 Xanthan Gum 0.05 6 Acrylates / C10-30 Alkyl Acrylate Crosspolymer 0.10 8 Caprylic / Capric Triglyceride 3.00 9 Cyclomethicone 5.00 10 Glyceryl Stearate, PEG-100 Stearate 2.00 11 Sorbitan Stearate 1.00 12 Cetyl Alcohol 0.50 13 Stearic Acid 0.50 14 Glyceryl Stearate SE 0.50 15 Polysorbate 60 0.50 16 Triethanolamine 0.14 17 Example A-4 or Example A-5 or Example A-6 1.00

≪ Example C > Preparation of Toner

Toner was prepared by using the composition for preserving cosmetics containing the blackcumin seed extract of the present invention as an active ingredient. Specifically, the toners of Examples C-1 to C-3 were prepared using the composition prepared in Example A, and the composition ratios thereof are shown in Table 3 below. The toner prepared in Example A-4 and the toner prepared in Example A-5 were the same as those in Example C-2 and Example A-6, Example C-3.

No Material name content (%) One Water 93.18 2 Dipropylene Glycol 2.5 3 Glycerin 2 4 Methtyl Gluceth20 One 5 Carbomer 0.05 6 Triethanolamine 0.05 8 Sodium PCA 0.1 9 PEG-60 Hydrogenated Castor Oil 0.1 10 Disodium EDTA 0.02 11 Example A-4 or Example A-5 or Example A-6 1.00

<Experimental Example 1> Evaluation of antimicrobial effect of the composition

MIC (Minimum Inhibitory Concentration) test was performed to evaluate the antibacterial effect of the composition for preserving cosmetics containing the blackcumin seed extract of the present invention as an active ingredient. The results are shown in Table 4 below .

Specifically, two kinds of bacteria and two fungi for each of the compositions of Examples A-1 to A-7 were subjected to broth dilution method using Tryptic soy broth and Saburaud dextrose broth medium to obtain the minimum inhibitory concentration (MIC ) Test. Two types of bacteria are Escherichia coli (ATCC 8739) and Staphlyococcus aureus (ATCC 6538), and two fungi are Candida albicans (ATCC 10231) and Aspergillus niger (ATCC 16404) . (C8), 1,2-hexanediol, ethylhexylglycerin, glycerin, phenoxyethanol, methylparaben (Comparative Examples 1 to 8) were used to compare the antibacterial activity The antimicrobial activity of each of two bacteria and two fungi was measured individually.

Material name Minimum inhibitory concentration (%) E. coli S. aureus Candida albicans Aspergillus niger Example A-1 0.4 0.1 0.125 0.1 Example A-2 0.4 0.2 0.125 0.1 Example A-3 0.4 0.2 0.15 0.125 Example A-4 0.4 0.05 0.05 0.05 Example A-5 0.4 0.05 0.05 0.05 Example A-6 0.4 0.05 0.025 0.05 Example A-7 0.5 0.075 0.05 0.05 Comparative Example 1 Black Cumin seed extract 0.06 0.06 0.01 0.01 Comparative Example 2 Glyceryl monocaprylate 0.2 0.05 0.125 0.05 Comparative Example 3 1,2-hexanediol 1.0 2.0 1.0 0.5 Comparative Example 4 Ethylhexyl glycerin 0.4 0.1 0.05 0.05 Comparative Example 5 1,3-butylene glycol 10.0 14.0 10.0 14.0 Comparative Example 6 glycerin 28.0 35.0 30.0 40.0 Comparative Example 7 Phenoxyethanol 0.3 0.6 0.3 0.3 Comparative Example 8 Methyl paraben 0.1 0.2 0.1 0.1

As a result of the minimum inhibitory concentration (MIC) test, it was confirmed that the composition of Example A of the present invention inhibited bacteria and fungi at a low concentration. In particular, it shows excellent preservative effects by inhibiting bacteria and fungi at a similar or better level than the chemical preservatives of Comparative Examples 5 to 8, which have been widely used as conventional preservatives. As a result, As shown in FIG. Particularly, phenoxyethanol and methylparaben of Comparative Examples 7 and 8 exhibited excellent antibacterial effect in Comparative Examples, but since they are highly irritating to the skin and used in recent years, they are also useful as natural preservatives have.

More specifically, when Examples A-2, A-4, A-5, A-6 and A-7 were compared, even when the blackcumin seed extract was added in the same manner, glyceryl monocaprylate, , 2-hexanediol, and ethylhexyl glycerin. Specifically, when 65 parts by weight of glyceryl monocaprylate and 30 parts by weight of 1,2-hexanediol were used (Example A-2), 45 parts by weight of glyceryl monocaprylate, When 50 to 60 parts by weight of glyceryl mono-caprylate and 35 to 45 parts by weight of 1,2-hexanediol were used than 50 parts by weight of hexanediol (Example A-7), the antibacterial effect was remarkable .

In addition, when Examples A-2 and A-3 are compared, when the composition ratio of glyceryl monocaprylate, 1,2-hexanediol, and ethylhexyl glycerin is the same, When contained in large amounts, the antibacterial activity against bacteria is the same, but the antimicrobial activity against fungi is more excellent.

From the above results, it can be seen that the composition of the present invention exhibits an optimum antimicrobial effect when each of the four compositions is contained at an appropriate composition ratio.

When the blackcumin seed extract of Comparative Example 1 alone is used, it exhibits the most excellent antibacterial effect. However, when used alone, there is a problem that the skin irritation is so severe that it can not be used in clinical practice (see Experimental Example 2) The compositions of Examples A-4 to A-6 of the present invention show better effects against fungi than the case of Example 2 alone, and the glyceryl monocaprylate is a chemical substance, and the present invention is a natural substance preservative The glyceryl monocaprylate is not intended for the purposes of the present invention.

Experimental Example 2 Skin stimulation test of composition

In order to evaluate the applicability of the composition for preserving cosmetics containing the blackcumin seed extract of the present invention as an active ingredient, a human body application test was conducted, and the results are shown below.

Specifically, this test was performed by Chungbuk Provincial Cosmetic Clinical Research Support Center and performed by the Standard of Operating Procedure (SOP) of Chungcheongbukdo Cosmetic Clinical Research Support Center. The test procedure is as follows.

1. Selection of test subjects

1) Selection Criteria

The following criteria were selected.

a. Male and female adults aged 20 to 55

(b) Those who have fully explained the matters to be disclosed to the subjects described by the State Examiner and who voluntarily signed and signed the Clinical Examination Attendance Agreement

c. Healthy person who does not have grade or chronic physical disease including skin disease

d. Those who can follow up during the test period

2) Exemption criteria

The following subjects were excluded from the test subjects by interviewing the subjects.

end. Pregnant or lactating women and women who are pregnant

I. A person who has been using a skin contour containing steroids for one month or more to treat skin diseases

All. Those who have not passed six months after taking the same exam. Persons with sensitive, irritable skin

hemp. Persons with skin abnormalities such as dots, acne, erythema, and capillary dilatation on the test site. Those who used the same or similar cosmetics or medicines on the test site within 3 months prior to the start of the study

four. Significant nutritionist

Ah. Patients who have received skin care or skin care within 3 months prior to the start of the study. Those who are deemed to be unsuitable for the examination by the State Expert

2. Test method

The test for evaluating the primary skin irritation reaction of cosmetics can be selected from among the 24 hour or 48 hour patches depending on the researcher's tendency or the characteristics of the test substance. In the case of the human body test, the stimulation response was evaluated by a reading process over a period of three hours after the 24-hour patch. In the case of 24-hour patches, two readings are generally performed. However, in the present test, repeated and continuous observation of the intensity of the reaction is repeated to overcome the technical limitations of the single-pouch test by evaluating the stimulation and sensitization of the Acute & Total 3 readings were selected for evaluation. The method used in this test was a patch adhering step to cause a skin irritation reaction and a visual evaluation step to skin irritation and sensitization by an expert.

1) Number of subjects

The test subjects were selected by 30 persons who did not have the exclusion criteria.

2) Test site

The test site was placed on the back of the subject.

3) Research schedule and procedures

end. Visit 1 (0 days, start date)

The test subjects were selected according to the test subjects' selection and exclusion criteria, and the skin condition of the test subjects was checked and tested according to the test methods.

I. Visit 2 (1 day, 30 minutes after patch removal)

Visual evaluation and photographing were carried out to determine the skin reaction according to the test method.

All. Visit 3 (2 days, after patch removal 24 hours)

Visual evaluation and photographing were carried out to determine the skin reaction according to the test method.

la. Visit 4 (3 days, 48 hours after patch removal)

Visual evaluation and photographing were carried out to determine the skin reaction according to the test method.

3. Measurement and evaluation methods

1) Preparation stage

For the evaluation, the subjects were stabilized for 30 minutes in a waiting room under constant temperature and humidity (22 ± 2 ℃, RH 40 ~ 60%) to adapt the skin surface temperature and humidity to the environment of the measurement space. The test site was photographed, and the skin response was confirmed by two skilled researchers under the same conditions each time.

2) Measurement step

end. Attach patch

The back part of the subject was wiped with 70% ethanol and dried. Then, 25 μl of the test product was dropped on IQ Ultra and fixed on the test site (etc.).

I. Check skin irritation

After 2, 3, and 4 hours, patch removal was performed using Frosch & Kligman's assessment method based on the reading criteria of the International Contact Dermatitis Research Group (ICDRG) after 30 minutes, 24 hours, and 48 hours The degree of stimulation was observed. The evaluation criteria are shown in Table 5 below. In order to obtain the objectivity of the visual evaluation, the degree of skin irritation is' Hee Cheol Eun, Byung Kook Kim, Seo Dae Heon and others. (A) Study on the results of adventitious agent test except for cosmetic and perfume antigens in contact dermatitis patients. Korean Journal of Dermatology 1997; 35 (2): 279-283.

Degree of stimulation Score Types of skin symptoms or signs ± 0.5 Doubtful skin sign such as weak erythema + One Slight erythema, either spotty or diffuse ++ 2 Moderate uniform erythema +++ 3 Intense erythema with edema ++++ 4 Intense erythema with edema & vesicles - (negative) - Negative reaction IR (irritant
reaction) - Other types of stimuli (including adhesive stimuli) NT (not tested) - Stop the test due to the occurrence of the stimulus reaction or other reasons

All. How to calculate skin irritation

The average stimulation index was calculated according to the skin irritation index calculation formula described below and the evaluation criteria according to the average stimulation index are shown in Table 6. 'S. M. An, H. Ham, E. J. Choi et al. Primary irritation index and safety zone of cosmetics: retrospective analysis of skin patch tests in 7440 Korean women during 12 years. Based on the product safety zone (Table 7) using mean stimulation index and standard deviation as shown in International Journal of Cosmetic Science, 2014, 36, 62-67, the mean response index R value of z ≤ 1.00 is shown in Table 7 , The final product was judged to be a safe product and predicted to be highly likely to induce skin irritation in the case of use beyond the predicted value.

[formula]

A: The sum of observations at each reading - Σ (Each Score: 0.5, 1, 2, 3, 4)

B: The maximum score of skin irritation used in the test

C: Total number of subjects participating in the test

D: total read degree

Mean skin irritation index Judgment grade 0.00 to 0.86 Slight grade 0.87 to 2.43 Mild grade 2.42-3.43 Moderate grade 3.44 ~ Severe grade

la. Predicting primary stimulation potential

Cosmetics are not stimulated only by the contents of the product itself and the skin, but there is a possibility that skin irritation may be caused by various factors such as actual product use behavior, race, climate, skin condition of the subject The safety test is called the prediction test (Prediction test) and the safety can not be emphasized any more.

In the case of the stimulation patch test currently being conducted due to the skin irritation phenomenon caused by various factors such as cosmetics, there is no criterion for the suitability of the globally known products. However, according to recent research results, based on the results of 10 years patches for Korean women, it may be possible to use a safety zone considering the use of actual products and use it as a criterion for the possibility of stimulation.

In addition, in general, when a test is performed for a test subject of 50 or less subjects, a test product in which a reaction occurs in a frequency exceeding 20% The test product observed in more than 10% of the test subjects can be predicted as a test product which is likely to cause significant stimulation.

Therefore, in the case of this test, the safety zone (Table 7), the stimuli-induced product with a frequency of more than 20% of the test subjects, the test product in which more than +2 stimuli are read at 10% In the case of one, it was judged to be a test product with potential for stimulation. In this case, the test product was judged as 'non-conforming'.

Product Category Safety zone R value of average reaction index with z ≤ 1.00 Residue
Leave-on



toner 0.87 Lotion and cream 1.12 foundation 0.97 Sunscreen 0.83 Lip product 0.98 Body lotion and cream 0.94 Face Powder, Eye Shadow 1.02 Eyeliner, mascara 2.96 Remove with tissue
Tissue-off Makeup remover 1.36 Pack and massage cream 1.33 Wash it out yesterday
Wash-off Facial Cleanser 3.20 Soap and bath salts 3.98 Shampoo, conditioner and hair treatment 2.82

- 30 X 0.2 = 6.0 Therefore, in the case of this test, the product stimulated by more than 6 subjects

- test products that were observed at> 10% of the total subjects

4. Test results

1) Average skin irritation index

The average stimulation index was calculated according to the evaluation criteria, and the skin irritation intensity was evaluated according to the judgment criteria. According to the product, the safety zone, the stimuli caused by the frequency of the test subject exceeding 20%, and the test product which is observed in more than 10% of the total subjects, And the final product of the product is predicted and shown in Table 8 below.

Name of sample Sum of skin irritation reaction intensity skin
Stimulation
Indices skin
Irritation degree
Judgment
Rating Safety
zone exam
Subject
20%
Excess +2
More than
10%
More than Compulsory
Judgment Primary Secondary Third Example A-6 0.50 0.00 0.00 0.14 slightly fitness fitness fitness fitness Comparative Example 1 24.50 17.50 12.50 15.14 Severe incongruity incongruity incongruity incongruity DW
(Voice contrast) 0.00 0.00 0.00 0.00 slightly fitness fitness fitness fitness 1% SLS
(Positive contrast) 5.00 5.00 2.50 3.47 Severe fitness incongruity fitness incongruity

As shown in the above table,

The composition for preserving cosmetics containing the blackcumin seed extract of the present invention as an active ingredient has a skin irritation index of 0.14 which is significantly smaller than that of the positive control group and is superior to that of the blackcumin seed extract alone Or less. &Lt; / RTI &gt;

From this, it can be seen that the blackcumin seed extract alone can not be used alone because of its strong skin irritation.

<Experimental Example 3> Evaluation of the buoyancy of cosmetics containing the composition

In order to evaluate whether or not a cosmetic composition containing the blackcumin seed extract of the present invention as an active ingredient was applied to a cosmetic product and exhibited a preservative effect, the antifouling strength of the lotion and toner prepared in Examples B and C , And the results are shown in Table 9 and Figs. 1 and 2.

Specifically, the lotion and the toner were fixed at the same total 1% in order to compare the flushing force of the lotion with the six kinds of toners prepared in Experimental Examples B and C, and a challenge test was performed. Experimental methods were tested according to "Preservation Testing of Cosmetics" in CTFA Technical Guide. E. coli (ATCC 8739), P. aeruginosa (ATCC 9027) and S. aureus (ATCC 25923) were used for the inoculated bacteria, and Candida albicans (ATCC 10231) and Aspergillus niger (ATCC 16404) were used for the fungi. The number of bacteria was measured after 3 days, 7 days, 14 days, and 28 days in the judgment criteria, and the results are shown in Table 9 below.

Cosmetics Elapsed Days 0 days 3 days 7 days 14 days 28th Example B-1
1% applied bacteria + - - - - mold + + + + - Example B-2
1% applied bacteria + - - - - mold + + + + - Example B-3
1% applied bacteria + - - - - mold + + + - - Example C-1
1% applied bacteria + - - - - mold + + + + - Example C-2
1% applied bacteria + - - - - mold + + + + - Example C-3
1% applied bacteria + - - - - mold + + + + -

-: Less than 100 CFU / ml of contaminating microorganism

+: 100CFU / ml or more of contaminated microorganisms

BRIEF DESCRIPTION OF THE DRAWINGS Fig. 1 is an image showing the result of evaluating the flushing force of a lotion prepared using a composition for preserving cosmetics containing an extract of blackcumin seed as an active ingredient. Fig.

Fig. 2 is an image showing the result of evaluating the antifouling force of a toner prepared by using a composition for preserving cosmetics containing a blackcumin seed extract as an active ingredient. Fig.

As shown in Table 9, Figs. 1 and 2,

In the case of bacteria (bacteria), the contaminant microorganism on day 0 was at least 100 CFU / ml but fell below 100 CFU / ml from day 3, indicating that the cosmetic preserving composition of the present invention is excellent in antibacterial effect,

In the case of mold (fungus), the contaminating microorganism was at least 100 CFU / ml from day 14, but fell below 100 CFU / ml from day 28, indicating that the cosmetic preserving composition of the present invention has excellent antifungal effect.

Accordingly, the composition for preserving cosmetics containing the blackcumin seed extract of the present invention as an active ingredient can be usefully used as a preservative or preservative in cosmetics by applying it to cosmetics.

Claims (9)

Glyceryl mono fatty acid esters; At least one polyhydric alcohol selected from the group consisting of 1,2-hexanediol, pentylene glycol, 1,2-octanediol, ethylhexyl glycerin, 1,3-butyleneglycol and glycerin; And a Nigella sativa seed extract, which is a composition for preserving cosmetics,
The glyceryl mono fatty acid ester is contained in the composition in an amount of 50 to 60 parts by weight, the polyhydric alcohol is contained in 40 to 50 parts by weight,
Wherein the blackcumin seed extract is contained in an amount of 0.1 to 0.5 parts by weight based on 100 parts by weight of the total of the glyceryl mono fatty acid ester and the polyhydric alcohol.
The method according to claim 1,
Wherein the glyceryl mono fatty acid ester contains a glycerin derivative of C _8-18 , and exhibits an antibacterial or antifungal effect.
The method according to claim 1,
The glyceryl mono fatty acid ester is selected from the group consisting of glyceryl monocaprylate (C8), glyceryl monolaurate (C12), glyceryl monomyristate (C14), glyceryl monopalmitate (C16), glyceryl monostearate C18), and glyceryl monooleate (C18: 1). The composition for preserving cosmetics according to claim 1, wherein the cosmetic composition has an antibacterial or antifungal effect.
The method according to claim 1,
Wherein the blackcumin seed extract is obtained by using any one selected from the group consisting of C _1-4 lower alcohols and water or a combination thereof as an extraction solvent, wherein the composition exhibits an antibacterial or antifungal effect.
The method according to claim 1,
The cosmetic may be any one selected from the group consisting of lotion, toner, skin, lotion, emulsion, cream, essence, serum, gel, and pack; Make-up cosmetics selected from lipsticks, tints, lip gloss, lip balm, viscose cream, foundation, powder, mascara, eye shadow and eyeliner; Skin exfoliation scrub, cleansing water, cleansing oil, cleansing cream and foam cleansing; Hair cosmetics, hair cosmetics, hair shampoos, rinse, and treatments; And body cosmetics selected from the group consisting of body scrubs, body lotions, body creams, and body creams.
delete The method according to claim 1,
Wherein the bacterium is at least one selected from the group consisting of Escherichia coli and Staphlyococcus aureus and the fungus is selected from the group consisting of Candida albicans and Aspergillus niger Wherein the cosmetic composition exhibits an antibacterial or antifungal effect.
Glyceryl mono fatty acid esters; At least one polyhydric alcohol selected from the group consisting of 1,2-hexanediol, pentylene glycol, 1,2-octanediol, ethylhexyl glycerin, 1,3-butyleneglycol and glycerin; And a Nigella sativa seed extract, which is a composition for preserving cosmetics,
The glyceryl mono fatty acid ester is contained in the composition in an amount of 50 to 60 parts by weight, the polyhydric alcohol is contained in 40 to 50 parts by weight,
Wherein the blackcumin seed extract is contained in an amount of from 0.1 to 0.5 parts by weight based on 100 parts by weight of the total of the glyceryl mono fatty acid ester and the polyhydric alcohol in an amount of 0.01 to 5 parts by weight / RTI &gt;
9. The method of claim 8,
Wherein the cosmetic composition is any cosmetic composition selected from the group consisting of lotion, toner, skin, lotion, emulsion, cream, essence, serum, gel, and pack; Make-up cosmetics selected from lipsticks, tints, lip gloss, lip balm, viscose cream, foundation, powder, mascara, eye shadow and eyeliner; Skin exfoliation scrub, cleansing water, cleansing oil, cleansing cream and foam cleansing; Cosmetics for hair, which is selected from the group consisting of shampoos, rinses and treatments; And a cosmetic for body which is any one selected from the group consisting of body scrubs, body lotions, body creams, and body creams, and at least one cosmetic composition selected from the group consisting of body creams, body lotions, body creams and body creams.

Images