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CN116893502B - Optical module and method for projecting split image pattern for microscope - Google Patents

Optical module and method for projecting split image pattern for microscope Download PDF

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Publication number
CN116893502B
CN116893502B CN202310958522.1A CN202310958522A CN116893502B CN 116893502 B CN116893502 B CN 116893502B CN 202310958522 A CN202310958522 A CN 202310958522A CN 116893502 B CN116893502 B CN 116893502B
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split image
light source
wedge
shaped mirror
lens
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CN116893502A (en
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陈家乐
张旭
浦栋麟
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Jiangsu Jihui Huake Intelligent Equipment Technology Co ltd
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Jiangsu Jihui Huake Intelligent Equipment Technology Co ltd
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    • GPHYSICS
    • G02OPTICS
    • G02BOPTICAL ELEMENTS, SYSTEMS OR APPARATUS
    • G02B21/00Microscopes
    • G02B21/36Microscopes arranged for photographic purposes or projection purposes or digital imaging or video purposes including associated control and data processing arrangements
    • G02B21/361Optical details, e.g. image relay to the camera or image sensor
    • GPHYSICS
    • G02OPTICS
    • G02BOPTICAL ELEMENTS, SYSTEMS OR APPARATUS
    • G02B21/00Microscopes
    • G02B21/06Means for illuminating specimens
    • G02B21/08Condensers
    • GPHYSICS
    • G02OPTICS
    • G02BOPTICAL ELEMENTS, SYSTEMS OR APPARATUS
    • G02B21/00Microscopes
    • G02B21/24Base structure
    • G02B21/241Devices for focusing
    • G02B21/244Devices for focusing using image analysis techniques

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  • Physics & Mathematics (AREA)
  • Chemical & Material Sciences (AREA)
  • Analytical Chemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Optics & Photonics (AREA)
  • Engineering & Computer Science (AREA)
  • Computer Vision & Pattern Recognition (AREA)
  • Multimedia (AREA)
  • Microscoopes, Condenser (AREA)

Abstract

The invention relates to the technical field of automatic measurement microscopes, in particular to an optical module for projecting split image patterns for a microscope, which comprises a scattering light source, a wedge-shaped mirror array, a pattern template, a first lens component, a beam-splitting prism, a second lens component and a third lens component, wherein the pattern template is arranged on the back of the wedge-shaped mirror array; the scattered light source can emit a point light source, the light source forms a clear pattern aligned left and right on the confocal plane after passing through the wedge-shaped mirror array, and when the camera is out of focus, a left-right staggered image can be acquired, so that the camera is used for assisting in focusing. The invention optimizes the pattern projected by the split image method, so that the optical module can be applied to three-dimensional reconstruction based on the focusing method.

Description

Optical module and method for projecting split image pattern for microscope
Technical Field
The invention relates to the technical field of automatic measurement microscopes, in particular to an optical module and a method for projecting split image patterns for a microscope.
Background
The camera autofocus technique is an important application in the machine vision field, in the microscope autofocus technique, the sharpness is generally determined by means of the image gradient of the measured object, so as to determine whether to focus, in the practical application, if the surface texture of the measured object has uncertainty, it is quite possible that the surface texture is weaker, whether to focus can not be determined by the self texture, so that the split image method assisted focusing technique is introduced, that is, two bilateral symmetrical patterns are actively projected on the surface of the measured object, the two bilateral patterns can move up and down along with the focusing change, and when the two patterns are aligned, the focusing is completed.
The existing microscopic image splitting method only projects a pattern in a fixed local area in a camera view field, so that the auxiliary focusing technology is only effective in the area where the left split image pattern and the right split image pattern meet, and is suitable for evaluating a plane object placed horizontally, and if a measured object has a certain height difference, the microscopic image splitting method cannot act on the area outside the projected pattern.
The three-dimensional morphology reconstruction under the microscope is an important application in the field of machine vision, and the three-dimensional reconstruction technology based on a focusing method can finish depth reconstruction by only relying on one camera, and is suitable for a use scene of the microscope. The technology needs to collect a group of image sequences at different heights, calculate the definition values of the local area at different heights through a focusing evaluation operator so as to draw a definition-depth curve, and obtain the depth of the area by judging the position of the wave crest.
However, the three-dimensional reconstruction technique based on the focusing method is seriously dependent on texture information of the surface of the object, and in practical application, because the object under the microscope has some areas with noise or poor texture, in these areas, the focus value calculated by the focus evaluation operator from each image is very low, and the peak value is difficult to find, so the depth of the reconstruction of the area tends to be low in accuracy. In combination with the principle of the split image method, a template pattern can be actively projected to strengthen the texture of the surface of an object, so that the accuracy of three-dimensional reconstruction of a focusing method is improved, but the pattern template used by the existing split image method is not suitable for three-dimensional reconstruction of the focusing method. In view of the above problems, the design of an optical module which can enable the split image method to act on the whole view field of the camera and can be used for assisting the three-dimensional reconstruction technology of the focusing method is of great significance to improving the automatic focusing technology and the three-dimensional reconstruction technology of the focusing method under a microscope.
Disclosure of Invention
The invention provides an optical module and a method for projecting split image patterns for a microscope, which aim to solve the technical problems in the background technology.
The technical scheme of the invention is as follows: the optical module for projecting split image patterns for microscopes comprises a scattering light source, a wedge-shaped mirror array, a pattern template, a first lens assembly, a beam splitting prism, a second lens assembly and a third lens assembly, wherein the wedge-shaped mirror array is arranged at a light source outlet of the scattering light source, the pattern template is arranged at the back of the wedge-shaped mirror array, the first lens assembly is arranged between the beam splitting prism and the wedge-shaped mirror array, the second lens assembly is arranged between the beam splitting prism and an object to be measured, the third lens assembly is arranged between the beam splitting prism and a camera, and the object to be measured is positioned on a focusing surface of the second lens assembly;
The scattered light source can emit uniform point light sources which are distributed in a plane, the point light sources form split image pattern light sources through the wedge-shaped mirror array and the pattern template, the first lens component refracts the split image pattern light sources into parallel light, the split image pattern light sources are reflected to the second lens component by the beam splitting prism, the split image patterns are projected on a focusing plane by the second lens component, and the camera collects a tested object with the split image patterns through the third lens component.
Further, the wedge-shaped mirror array comprises a plurality of wedge-shaped mirror units which are arranged in an array mode, and the split image pattern of the pattern template corresponds to the wedge-shaped mirror units which are arranged in the array mode.
Further, the wedge-shaped mirror unit comprises two triangular prisms, and the two triangular prisms are installed in a staggered and opposite mode.
Further, the split image pattern of the pattern template is square grids which are arranged in an array mode, and the side length of each square grid is smaller than half of the size of a focusing evaluation operator window in a focusing method.
Further, the second lens component and the third lens component are lenses with the same specification.
Further, the wedge mirror array is located at a focal length of the first lens assembly.
Further, the scattered light source comprises a light source, a lens and a light guide assembly, wherein the lens is arranged between the light source and the light guide assembly, the lens is used for converting light rays emitted by the light source into parallel light, and the light guide assembly is used for converting the parallel light into uniform point light sources.
Further, the light guide assembly adopts ground glass.
Another technical scheme of the invention is as follows: a method for projecting split image patterns in a microscope, which adopts any one of the optical modules for projecting split image patterns for a microscope, comprising:
s10: emitting uniform point light sources which are distributed in a plane through a scattering light source;
s20: generating a split image pattern light source by the point light source through the wedge-shaped mirror array and the pattern template;
s30: refracting split image pattern light sources emitted from the wedge-shaped mirror array into parallel light sources through a first lens assembly;
s40: the split image pattern light source is reflected by the measured object after passing through the beam splitting prism and projected onto the focusing surface through the second lens component;
S50: when the measured object is positioned on the focusing surface, the measured object and the projected split image pattern are reflected to the camera, and the camera collects an image obtained by overlapping the measured object and the split image pattern through the third lens assembly.
Further, it further includes the steps performed before step S10:
the light emitted by the light source is converted into parallel light through the lens, and the parallel light is converted into uniform point light sources through the light guide assembly.
The invention has the beneficial effects that: according to the invention, the wedge-shaped mirror array and the pattern template are combined with the scattering light source, the first lens component, the beam splitter prism, the second lens component and the third lens component, so that the problem that the traditional splitting image method can only be applied to a planar object is solved, the application range of the splitting image method is wider, the pattern projected by the splitting image method is optimized, and the optical module can be applied to three-dimensional reconstruction based on the focusing method.
Drawings
Fig. 1 is a schematic view of the overall structure of the present invention.
Fig. 2 is a structural example of a wedge mirror unit of the present invention.
Fig. 3 is a schematic diagram of a pattern template of the present invention.
Fig. 4 is a flow chart of the present invention.
Detailed Description
In order that those skilled in the art will better understand the present invention, a technical solution in the embodiments of the present invention will be clearly and completely described in the following with reference to the accompanying drawings, in which the described embodiments are only some embodiments of the present invention, and not all embodiments. All other embodiments, which can be made by those skilled in the art based on the embodiments of the present invention without making any inventive effort, shall fall within the scope of the present invention.
In an embodiment of the present invention, fig. 1 is a schematic structural diagram provided by a specific structure of an optical module for projecting a split image pattern for a microscope according to the present invention, and as shown in fig. 1, the present invention specifically includes: the light source comprises a scattering light source 10, a wedge-shaped mirror array 20, a pattern template 30, a first lens assembly 40, a beam splitting prism 50, a second lens assembly 60 and a third lens assembly 70, wherein the wedge-shaped mirror array 20 is arranged at a light source emission port of the scattering light source 10, the pattern template 30 is arranged at the back of the wedge-shaped mirror array 20, and the pattern template is required to be tightly attached to the wedge-shaped mirror array, so that light rays transmitted by the wedge-shaped mirrors have template patterns. The first lens assembly 40 is mounted between the splitting prism 50 and the wedge-shaped mirror array 20, in particular, the wedge-shaped mirror array 20 is located at a focal length of the first lens assembly 40.
The second lens assembly 60 is installed between the beam splitting prism 50 and the object 80 to be measured, the third lens assembly 70 is installed between the beam splitting prism 50 and the camera 90, and the object 80 to be measured is located on the focusing surface of the second lens assembly 60. The second lens assembly 60 and the third lens assembly 70 adopt lenses with the same specification, the second lens assembly 60 and the third lens assembly 70 are respectively positioned at the upper side and the lower side of the beam splitting prism 50, and the second lens assembly 60 and the third lens assembly 70 are coaxially arranged.
The light source propagation path of the present invention is: the scattering light source 10 can emit uniform point light sources which are distributed in a plane, the point light sources form split image pattern light sources through the wedge-shaped mirror array 20 and the pattern template 30, the first lens component 40 refracts the split image pattern light sources into parallel light, the split image pattern light sources are reflected to the second lens component 60 by the beam splitting prism 50, the split image pattern is projected on a focusing surface by the second lens component 60, and the camera 90 collects a measured object 80 with the split image pattern through the third lens component 70.
The wedge-shaped mirror array 20 includes a plurality of wedge-shaped mirror units arranged in an array, and the split image pattern of the pattern template 30 corresponds to the wedge-shaped mirror units arranged in the array. A plurality of wedge-shaped mirror units are arranged and combined in a rectangular mode to form a wedge-shaped mirror array, so that a plurality of independent split image patterns can be formed on a confocal plane, and the area of each split image pattern can cover the whole camera field of view. The split image pattern is generated by using the wedge-shaped mirror array and the pattern template, the wedge-shaped mirror array is arranged at a position which is one time away from the lens, the light rays pass through the wedge-shaped mirror and form clear patterns which are aligned left and right on the focusing surface, and when the camera is out of focus, the left and right staggered images can be acquired, so that the auxiliary focusing of the camera can be realized.
Specifically, as shown in fig. 2, the wedge-shaped mirror unit includes two triangular prisms, and the two triangular prisms are installed in a staggered and opposite manner, so that the left and right template patterns projected when the camera is out of focus can move in different directions, so as to realize image dislocation. As shown in fig. 3, the split image pattern of the pattern template 30 is square grids arranged in an array, and the square grids can be drawn by using a metal plating layer, so that the pattern part has high reflectivity and low light transmittance. The positions of each square are in one-to-one correspondence with the positions of the wedge-shaped mirror units, so that the projected patterns of each wedge-shaped mirror unit are the same. The template pattern can be used for three-dimensional reconstruction based on a focusing method, so that the side length of the square is less than half of the window size of a focusing evaluation operator in the focusing method, and the drawn square side length is 10 micrometers.
In one embodiment of the present disclosure, as shown in fig. 1, the diffuse light source 10 includes a light source 101, a lens 102 and a light guide assembly 103, the lens 102 is installed between the light source 101 and the light guide assembly 103, the lens 102 is used to convert light emitted from the light source 101 into parallel light,
In order to make the intensity of the light incident from each wedge-shaped mirror unit in the wedge-shaped mirror array the same, a lens 102 is used to change the point light source from the light source 101 into a uniform parallel light source. The light guide assembly 103 is used for converting parallel light into uniform point light sources, wherein the light guide assembly 103 adopts ground glass. Before the light passes through the wedge-shaped mirror array, the frosted glass is used for modulating the parallel light source in the step 3, and the parallel light source is scattered at the frosted glass, so that uniform point light sources distributed in a plane can be generated, and the light source finally entering each wedge-shaped mirror unit is the uniform point light source. It should be noted that the lens 102 and the first lens assembly 40 in the present embodiment use the same specification of lens.
Another technical scheme of the invention is as follows: a method for projecting split image patterns in a microscope, which adopts any one of the optical modules for projecting split image patterns for a microscope, comprising:
s10: emitting uniform point light sources distributed in a plane through the scattering light source 10;
S20: the point light source generates a split image pattern light source through the wedge mirror array 20 and the pattern template 30;
s30: refracting the split image pattern light source emitted from the wedge mirror array 20 into parallel light sources by the first lens assembly 40;
s40: the split image pattern light source is reflected by the measured object 80 after passing through the beam splitting prism 50 and projected onto a focusing surface through the second lens assembly 60;
S50: when the measured object 80 is located on the focusing plane, the measured object 80 and the projected split image pattern are reflected to the camera 90, and the camera 90 collects the image after the measured object 80 and the split image pattern are overlapped through the third lens assembly 70.
Before step S10, it is necessary to perform: the light emitted from the light source 101 is converted into parallel light by the lens 102, and the parallel light is converted into a uniform point light source by the light guide assembly 103.
The beneficial effects of the technical scheme are the same as those of an optical module for projecting split image patterns for a microscope, so that the description is omitted here.
Finally, it should be noted that the above-mentioned embodiments are only for illustrating the technical solution of the present invention, and not for limiting the same, and although the present invention has been described in detail with reference to examples, it should be understood by those skilled in the art that modifications and equivalents may be made to the technical solution of the present invention without departing from the spirit and scope of the technical solution of the present invention, and all such modifications and equivalents are intended to be encompassed in the scope of the claims of the present invention.

Claims (7)

1. An optical module for projecting split image patterns for microscopes is characterized by comprising a scattering light source (10), a wedge-shaped mirror array (20), a pattern template (30), a first lens component (40), a beam-splitting prism (50), a second lens component (60) and a third lens component (70), wherein the wedge-shaped mirror array (20) is arranged at a light source outlet of the scattering light source (10), the pattern template (30) is arranged at the back of the wedge-shaped mirror array (20), the first lens component (40) is arranged between the beam-splitting prism (50) and the wedge-shaped mirror array (20), the second lens component (60) is arranged between the beam-splitting prism (50) and an object to be measured (80), the third lens component (70) is arranged between the beam-splitting prism (50) and a camera (90), and the object to be measured (80) is positioned on a focusing surface of the second lens component (60);
The scattering light source (10) can emit uniform point light sources which are distributed in a plane, the point light sources form split image pattern light sources through the wedge-shaped mirror array (20) and the pattern template (30), the first lens component (40) refracts the split image pattern light sources into parallel light, the split image pattern light sources are reflected to the second lens component (60) by the beam splitting prism (50), the split image patterns are projected on a focusing plane by the second lens component (60), and the camera (90) collects a measured object (80) with the split image patterns through the third lens component (70);
The wedge-shaped mirror array (20) comprises a plurality of wedge-shaped mirror units which are arranged in an array manner, and the split image pattern of the pattern template (30) corresponds to the wedge-shaped mirror units which are arranged in the array manner;
the wedge-shaped mirror unit comprises two triangular prisms which are arranged in a staggered and opposite way;
The split image pattern of the pattern template (30) is square grids which are arranged in an array mode, and the side length of each square grid is smaller than half of the size of a focusing evaluation operator window in a focusing method.
2. The optical module for projecting split image patterns for microscopes according to claim 1, wherein the second lens assembly (60) and the third lens assembly (70) are lenses of the same specification.
3. An optical module for projecting split image patterns for microscopes as claimed in claim 1, characterized in that said wedge mirror array (20) is located at a focal length of the first lens assembly (40).
4. The optical module for projecting a split image pattern for a microscope according to claim 1, wherein the scattered light source (10) comprises a light source (101), a lens (102) and a light guide assembly (103), the lens (102) is installed between the light source (101) and the light guide assembly (103), the lens (102) is used for converting light rays emitted by the light source (101) into parallel light, and the light guide assembly (103) is used for converting the parallel light into uniform point light sources.
5. The optical module for projecting split image patterns for microscopes according to claim 4, wherein the light guide member (103) is made of ground glass.
6. A method for projecting split image patterns in a microscope, characterized in that an optical module for projecting split image patterns for a microscope according to claim 4 or 5 is used, comprising:
s10: emitting uniform point light sources distributed in a plane through a scattering light source (10);
s20: the point light source generates a split image pattern light source through the wedge-shaped mirror array (20) and the pattern template (30);
S30: refracting the split image pattern light source emitted from the wedge-shaped mirror array (20) into a parallel light source by a first lens assembly (40);
s40: the split image pattern light source is reflected by the measured object (80) after passing through the beam splitting prism (50) and projected onto the focusing surface through the second lens component (60);
S50: when the measured object (80) is positioned on the focusing surface, the measured object (80) and the projected split image pattern are reflected to the camera (90), and the camera (90) collects an image after the measured object (80) and the split image pattern are overlapped through the third lens assembly (70).
7. The method of projecting a split image pattern in a microscope as claimed in claim 6, further comprising, performed prior to step S10:
light emitted by the light source (101) is converted into parallel light through the lens (102), and the parallel light is converted into uniform point light sources through the light guide assembly (103).
CN202310958522.1A 2023-07-31 2023-07-31 Optical module and method for projecting split image pattern for microscope Active CN116893502B (en)

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Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN208421405U (en) * 2018-06-26 2019-01-22 吉林鸿锋机械电子设备有限公司 A kind of heavy caliber parallel light tube focal plane high-precision is split as autocollimatic marking apparatus
CN113467065A (en) * 2021-07-06 2021-10-01 深圳市卡提列光学技术有限公司 Automatic focusing system

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE2361692C3 (en) * 1973-03-02 1981-10-15 C. Reichert Optische Werke Ag, Wien Microscope with at least one photographic camera
US7813579B2 (en) * 2004-05-24 2010-10-12 Hamamatsu Photonics K.K. Microscope system
DE102008000116A1 (en) * 2008-01-21 2009-07-30 Seereal Technologies S.A. Illumination unit for a holographic reconstruction system

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN208421405U (en) * 2018-06-26 2019-01-22 吉林鸿锋机械电子设备有限公司 A kind of heavy caliber parallel light tube focal plane high-precision is split as autocollimatic marking apparatus
CN113467065A (en) * 2021-07-06 2021-10-01 深圳市卡提列光学技术有限公司 Automatic focusing system

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