CN116803404B - Application of water-soluble tomato concentrate in preparation of hair loss reducing and hair growing products - Google Patents
Application of water-soluble tomato concentrate in preparation of hair loss reducing and hair growing products Download PDFInfo
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Abstract
The application relates to application of water-soluble tomato concentrate in preparing a product for reducing alopecia and growing hair. Researchers of the application find through research that the water-soluble tomato concentrate can promote proliferation of hair papilla cells; and inhibit apoptosis of papilla cells; promoting the expression of IGF-1 by active factors and reducing the expression of TGF-beta 1, and the water-soluble tomato concentrate has the effects of promoting the hair follicle to maintain the growing period and inhibiting the entering of the retrograde period, so that the water-soluble tomato concentrate has good effects of preventing alopecia and promoting hair growth. The application provides a new use for water-soluble tomato concentrate, and a new product for reducing hair loss and/or hair growth.
Description
Technical Field
The application relates to the technical field of biological medicine, in particular to application of a water-soluble tomato concentrate in preparing a product for reducing alopecia and growing hair.
Background
Hair is a natural high molecular keratin fiber, which can be divided into four parts of hair papilla, hair follicle and Mao Genhe hair shaft from bottom to top. It is chemically inert but sensitive to light, boiling water, acids, bases, oxidants and reductants. The physiological characteristics and functions of hair are mainly determined by the papilla, hair follicle, sebaceous glands, etc. below the scalp epidermis.
Hair follicles are constantly replaced in the cycle of each of the catagen and telogen phases of the growing phase, and there is constant new hair growth. The hair papilla is located at the basal part of the hair follicle bulb, is the most important dermal component of the hair follicle, and consists of important structures such as hair papilla cells, extracellular matrix, vascular nerves and the like. It not only plays an important regulatory role in the follicular cycle, but also maintains and induces it.
Currently, many products for treating and preventing alopecia comprise western medicines and traditional Chinese medicines. The western medicine products are mainly oral finasteride and external minoxidil, which are both hair restorer approved by FDA, and have obvious effect, but have higher price and obvious side effects, such as finasteride is easy to cause male dysfunction, minoxidil is easy to cause dermatitis and allergy, and drug resistance is easy to generate after long-term use; most of traditional Chinese medicine products have complex composition and slow effect. Therefore, it is necessary to provide a new drug which can effectively solve the problem of alopecia.
Disclosure of Invention
Based on the above, the application provides a new application of the water-soluble tomato concentrate, and relates to the application of the water-soluble tomato concentrate in preparing products for reducing alopecia and growing hair.
The specific technical scheme is as follows:
in a first aspect of the application there is provided the use of a water-soluble tomato concentrate in the manufacture of a product for reducing hair loss and/or hair growth.
In some of these embodiments, the reducing hair loss and/or growing hair comprises promoting proliferation of hair papilla cells.
In some embodiments, the reducing hair loss and/or growing hair comprises inhibiting apoptosis of hair papilla cells.
In some of these embodiments, the reducing hair loss and/or growing hair comprises promoting expression of the active factor IGF-1.
In some of these embodiments, the reducing hair loss and/or hair growth comprises reducing expression of the active factor TGF- β1.
In some embodiments, the product further comprises other substances beneficial to hair growth and hair care, including one or more of cowberry fruit extract, grape seed extract, saw palmetto extract, wheat germ extract, semen Cuscutae extract, ginseng radix extract, flos Matricariae Chamomillae extract, curcuma rhizome extract, herba Silybi Mariani extract, semen Panici Miliacei extract, oleum Lini, DHA, EPA, and vitamin E.
In some embodiments, the hair loss and/or hair growth reducing product dosage form is an external dosage form or an internal dosage form, including solutions, emulsions, and suspensions.
In some of these embodiments, the internal dosage forms include solutions, powders, tablets, granules, capsules, emulsions, suspensions, gel candies, and flakes.
In some of these embodiments, the product further comprises a health food or pharmaceutically acceptable adjuvant.
In some embodiments, the adjunct includes one or more of a flavor, a preservative, a flavoring agent, a thickener, a binder, a disintegrant, a lubricant, and a diluent.
Researchers of the application find through research that the water-soluble tomato concentrate can promote proliferation of hair papilla cells; and inhibit apoptosis of papilla cells; promoting the expression of IGF-1 by active factors and reducing the expression of TGF-beta 1, and the water-soluble tomato concentrate has the effects of promoting the hair follicle to maintain the growing period and inhibiting the entering of the retrograde period, so that the water-soluble tomato concentrate has good effects of preventing alopecia and promoting hair growth. The application provides new use of water-soluble tomato concentrate, and provides a new product for reducing alopecia and promoting hair growth.
The research shows that the water-soluble tomato concentrate is applied to the preparation of products for reducing alopecia and hair growth, has obvious drug effect and wide application prospect, and has great popularization and application value.
Drawings
FIG. 1 is a graph showing the TUNEL staining results of cells treated with water-soluble tomato concentrate at various concentrations in example 1;
FIG. 2 is a standard graph of the active factor VEGF in example 1;
FIG. 3 is the effect on the hair growth cycle of C57BL 6 mice observed after administration of the different components of example 1.
Detailed Description
The use of the water-soluble tomato concentrate of the present application for the preparation of hair loss and hair growth products is described in further detail below in connection with the specific examples. The present application may be embodied in many different forms and is not limited to the embodiments described herein. Rather, these embodiments are provided so that this disclosure will be thorough and complete.
Unless defined otherwise, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this application belongs. The terminology used herein in the description of the application is for the purpose of describing particular embodiments only and is not intended to be limiting of the application.
The term "and/or," "and/or," as used herein, includes any one of two or more of the listed items in relation to each other, as well as any and all combinations of the listed items in relation to each other, including any two of the listed items in relation to each other, any more of the listed items in relation to each other, or all combinations of the listed items in relation to each other.
In the present application, "first aspect", "second aspect", etc. are used for descriptive purposes only and are not to be construed as indicating or implying a relative importance or quantity, nor as implying an importance or quantity of the indicated technical features. Moreover, "first," "second," etc. are for non-exhaustive list description purposes only, and it should be understood that no closed limitation on the number is made.
In the application, the technical characteristics described in an open mode comprise a closed technical scheme composed of the listed characteristics and also comprise an open technical scheme comprising the listed characteristics.
The weights of the relevant components mentioned in the description of the embodiments of the present application may refer not only to the specific contents of the components, but also to the proportional relationship between the weights of the components, so long as the contents of the relevant components in the description of the embodiments of the present application are scaled up or down within the scope of the disclosure of the embodiments of the present application. Specifically, the weight described in the specification of the embodiment of the present application may be mass units known in the chemical industry field such as μ g, mg, g, kg.
In the present application, a numerical range (i.e., a numerical range) is referred to, and optional numerical distributions are considered to be continuous within the numerical range and include two numerical endpoints (i.e., a minimum value and a maximum value) of the numerical range and each numerical value between the two numerical endpoints unless otherwise specified. Where a numerical range merely refers to integers within the numerical range, including both end integers of the numerical range, and each integer between the two ends, unless otherwise indicated, each integer is recited herein as directly, such as where t is an integer selected from 1-10, and where t is any integer selected from the group of integers consisting of 1, 2, 3, 4, 5, 6, 7, 8, 9, and 10. Further, when a plurality of range description features or characteristics are provided, these ranges may be combined. In other words, unless otherwise indicated, the ranges disclosed herein are to be understood to include any and all subranges subsumed therein.
The temperature parameter in the present application is not particularly limited, and may be a constant temperature treatment or a treatment within a predetermined temperature range. The constant temperature process allows the temperature to fluctuate within the accuracy of the instrument control.
The water-soluble tomato concentrate (Water soluble tomato concentrate, WSTC) is obtained by extracting from ripe tomatoes, physically removing fat-soluble components from tomatoes, and concentrating the water-soluble active components, the preparation method of which is known to those skilled in the art. WSTC mainly contains nucleoside, phenol, flavonoid and other components beneficial to human body, researches show that the WSTC has the effects of resisting platelet aggregation and improving blood microcirculation, and the application of the water-soluble tomato concentrate in the prior art mainly focuses on preventing thrombotic cardiovascular diseases, and the application of the water-soluble tomato concentrate serving as an active ingredient in products for reducing alopecia and hair care is not reported.
The transformation of the hair follicle growth cycle at various stages is controlled by the interaction between the hair follicle keratinocytes (hair follicle epithelial cells) and dermal papilla fibroblasts, epithelial and mesenchymal tissues. Dermal papilla is essential for inducing and maintaining the periodic circulation of hair follicles, and its size (determined by the number of stroma cells in the hair bulb portion) determines the size of hair bulbs in the growing period, the length of the growing period, and the length and diameter of hair shafts. If the hair papilla is absent, or cannot be brought into close contact with the hair matrix keratinocytes thereabove, the growth phase will be difficult to maintain. Hair papilla cells are known to secrete a variety of growth factors and cytokines such as insulin-like growth factor (IGF) -1 (to maintain and promote hair growth), fibroblast Growth Factor (FGF) -7, FGF-5S (to delay the growth phase of hair follicles in the absence), hepatocyte Growth Factor (HGF) (to stimulate cellular DNA synthesis, promote the growth of a variety of epithelial and melanocytes, to lengthen the hair shaft), vascular Endothelial Growth Factor (VEGF) (to promote proliferation and migration of hair follicle cells), and the like to induce proliferation of matrix, dermal papilla vasculature cells, increase the number of extracellular matrix of dermal papilla, and maintain hair follicles in the growth phase.
The present application provides the use of a water-soluble tomato concentrate for the preparation of a product for reducing hair loss and/or hair growth.
In one specific example, reducing hair loss and/or growing hair includes promoting proliferation of hair papilla cells.
In one specific example, reducing hair loss and/or hair growth includes promoting expression of the active factor IGF-1.
In one specific example, reducing hair loss and/or hair growth comprises reducing expression of the active factor TGF- β1.
In some embodiments, the product further comprises other substances beneficial to hair growth and hair care, wherein the substances comprise one or more of cowberry fruit extract, grape seed extract, saw palmetto extract, wheat germ extract, semen Cuscutae extract, ginseng radix extract, flos Matricariae Chamomillae extract, curcuma rhizome extract and herba Silybi Mariani extract.
In some embodiments, the hair loss and/or hair growth reducing product dosage form is an external dosage form or an internal dosage form, and the external dosage form comprises a solution, an emulsion and a suspension.
In some embodiments, the aforementioned internal dosage forms include solutions, powders, tablets, granules, capsules, emulsions, suspensions, gel candies, and flakes.
In a specific example, the hair loss and/or hair growth reducing product is in the form of a solution consisting essentially of a water-soluble tomato concentrate and a solvent.
Specifically, the solvent is water, but is not limited thereto.
In a specific example, the dosage of the water-soluble tomato concentrate in the hair loss and/or hair growth reducing product is 62.5 μg/mL to 1000 μg/mL. Alternatively, the dosage of the water-soluble tomato concentrate in the hair loss reducing and/or hair growing product is 500 μg/mL. It will be appreciated that the specific concentration may be adjusted as desired depending on the condition or individual situation.
In some embodiments, the above-described products further comprise a health food or pharmaceutically acceptable adjuvant.
In some embodiments, the above-described adjuvants include one or more of fragrances, preservatives, flavoring agents, thickening agents, binders, disintegrants, lubricants, and diluents.
In some embodiments, the binder comprises one or more of povidone K30, copovidone, sodium carboxymethylcellulose, hydroxypropyl cellulose, and hypromellose.
In some embodiments, the lubricant comprises one or more of magnesium stearate, talc, and polyethylene glycols.
In some embodiments, the diluent comprises one or more of microcrystalline cellulose, isomalt, starch, dextrin, lactose, pregelatinized starch, mannitol, sorbitol, and erythritol.
In some embodiments, the disintegrant comprises one or more of crospovidone, sodium carboxymethyl starch, croscarmellose sodium, low substituted hydroxypropyl cellulose, and dry starch.
In some embodiments, the method for preparing the hair loss and/or hair growth reducing product comprises the following steps: mixing the water-soluble tomato concentrate with a solvent to prepare a product for reducing hair loss and/or hair growth. It will be appreciated that the method of preparation may be adapted as desired, depending on the dosage form or formulation.
The following are specific examples
Reagents and apparatus used in the examples, unless otherwise specified, are all routine choices in the art. The experimental methods without specific conditions noted in the examples were carried out according to conventional conditions, such as those described in the literature, books, or recommended by the manufacturer.
Example 1
1. Effect of water-soluble tomato concentrate on proliferation of dermal papilla cells
1.1 preparation of Water-soluble tomato concentrate
The method as described in patent "CN200980148421.6" of p Luo Weisi natural products limited: tomato extracts were prepared using as starting material a commercially available cold crushed tomato pulp having a Brix of 28-30 ° (i.e. 28-30% solids, w/w) of <0.350AU (Brix) with a browning index (absorbance at 420m of a solution with a concentration of 12.5g soluble solids/L). The slurry was diluted with ultrapure water (1:5) and large particulate matter was removed by centrifugation and then clarified at room temperature using a Westfalia MSB-14 separator (centrifugal disk clarifier). Smaller particulate material is then removed by microfiltration at a temperature of no more than 45 ℃ to give a clear pale yellow solution free of insoluble, sedimentable (spin-down) solids and capable of passing through a 0.2 μm filter without loss of soluble solids. The solution was concentrated by evaporation to a 65 ° brix slurry under carefully controlled conditions and at temperatures not exceeding 50 ℃ to limit non-enzymatic browning reactions. A rapid pasteurization step (t=105 ℃,3 seconds) was introduced at the beginning of the evaporation step. The final product was characterized by a browning index <0.600AU and a total plate count of microorganisms <1000.
In order to obtain a raw material containing a more concentrated bioactive ingredient, the sugar in the product is removed as described below. A column of L30L resin containing FPX66 resin (Rohm and Haas) was prepared and equilibrated in ultrapure water at 4 ℃. The above material was diluted to about 8 Brix with ultrapure water and passed through the resin column at a flow rate of about 260L/min, with the temperature maintained at 4 ℃. The column filtrate was discarded. Once all the required material has passed through the column, about 130L of flush water is passed through the column and discarded. Thereafter, the compounds that have remained on the resin are eluted by the following steps: 130L of hot water (75 ℃) was passed through the column, then 130L of 80% ethanol was passed through the column, and then another 130L of hot water was passed through the column. All eluting materials were retained and combined to give about 400L of an approximately 25% ethanol solution containing the compound of interest. The dilute solution containing the compound of interest was concentrated by reverse osmosis using a Trisep AC5 membrane at a temperature of about 30 ℃. The ethanol/water solvent passes through the membrane while all compounds dissolved therein remain within the membrane. Once the dilute solution was concentrated 10-fold, i.e. the volume was reduced to 40-50L, diafiltration was started, during which ultrapure water was added to the retentate at the same rate as the permeate removal rate. Thus, the ethanol concentration of the solution will gradually decrease from 25% to <5%.
The ethanol solution containing about 15-20% solids was then spray dried using an Anhydro spray dryer to give a golden fine powder with a water content of <6%, which is the final enriched water-soluble tomato extract.
The water-soluble tomato extract mainly comprises: (a) glycosylated phenolic acid or phenolic ester or derivative thereof; (b) glycosylated flavonoid; and (c) a nucleoside. The water-soluble tomato extract is separated from the pericarp, seed fragments, high molecular weight proteins, pectin and carotenoids (e.g. lycopene) by means of separation, and is a gaseous lipid stabilized in the form of droplets in an aqueous solution in the presence of pectin and proteins. I.e. the water-soluble tomato extract used in the present application is substantially free of lycopene.
1.2 principle of experiment
The CCK-8 cell viability detection kit (CK 04, dojindo, japan) contains WST-8 (2- (2-methoxy-4-nitrophenyl) -3- (4-nitrophenyl) -5- (2, 4-disulfophenyl) -2H-tetrazolium monosodium salt), and when the WST-8 is oxidized and reduced by intracellular dehydrogenase in the presence of an electronic carrier, water-soluble orange yellow formazan dye generated can be dissolved in a tissue culture medium, the color depth of the orange yellow formazan dye is in direct proportion to cell proliferation and in inverse proportion to cytotoxicity, the color depth of the orange yellow formazan dye is in linear relation to the cell number of the same cell, and the OD value is measured at the wavelength of 450nm by using an enzyme-labeled instrument, so that the number of living cells can be indirectly reflected.
1.3 Experimental methods
Taking cultured primary rat papilla cells, and adjusting cell suspension concentration to 5.0X10 4 And inoculating the cells to a 96-well cell culture plate for 24 hours per ml, and carrying out CCK8 experiments after the cells are attached. Adding culture medium containing 8 kinds of water-soluble tomato concentrates with different concentrations into experimental group, and parallel control group containing 5 kinds of common tomato powder with different concentrations (product of fructus Lycopersici Esculenti after heat crushing, pulping, vacuum concentrating, spray drying); the blank group is added with a culture medium without medicines, after incubation for 48 hours, CCK8 detection reagent is added, and after incubation is continued for 4 hours, OD value of each hole at 450nm wavelength is detected.
Cell proliferation rate calculation method: cell proliferation% = (experimental OD-blank OD)/(control OD-blank OD) ×100%.
1.4 experimental results
The experimental results of the effect of the water-soluble tomato concentrate on dermal papilla cell proliferation are shown in table 1.
TABLE 1
Note that: "/" indicates that proliferation was not detected.
As shown in Table 1, the water-soluble tomato concentrate can stimulate proliferation of hair papilla cells at 62.5-1000. Mu.g/mL, and the proliferation-promoting effect of 500. Mu.g/mL is optimal, compared with the control group, the proliferation-promoting rate of the water-soluble tomato concentrate is 188%, and the common tomato powder basically does not stimulate proliferation of hair papilla cells, and the high concentration can inhibit cell proliferation.
2. Effect of water-soluble tomato concentrate on reducing dermal papilla apoptosis
2.1 principle of experiment
Deoxyribonucleotide terminal transferase mediated gap end labeling (terminal-deoxynucleotidyl transferase mediated nick end labeling, TUNEL): when cells undergo apoptosis, some DNA endonucleases are activated, which cleave the genomic DNA between nucleosomes. Upon cleavage of genomic DNA, the exposed 3'-OH can be catalyzed by terminal deoxynucleotidyl transferase with the addition of green fluorescent probe Fluorescein (FITC) -labeled dUTP (Fluorescein-dUTP), so that apoptotic cells can be detected specifically and accurately by fluorescence microscopy or flow cytometry, while normal or proliferating cells have little cleavage of DNA and thus no 3' -OH formation and can be stained very rarely.
2.2 Experimental methods
Three optimal drug concentrations of 250 mug/mL, 500 mug/mL and 750 mug/mL obtained by CCK8 experiments are selected, the culture medium containing water-soluble tomato concentrate is added into an experimental group, the culture medium without drug is added into a blank group, and after incubation and culture for 48 hours, 125 mug/mL of common tomato powder is used in a parallel control group. Staining was performed with TUNEL staining kit (C1086, beyotime, china), and observation and photographing were performed with a fluorescence microscope.
2.3 experimental results
The apoptosis rate of the control group is 7.90+/-2.13%, the apoptosis rate of the tomato concentrate treated with 500 mug/mL is 3.42+/-1.48% (p < 0.05), the apoptosis rates of the tomato concentrate treated with 250 mug/mL and 750 mug/mL are 8.49+/-2.45% (p > 0.05) and 24.40+/-7.13%; the apoptosis rate of the common tomato powder treated with 125 mug/mL is 8.21+/-1.54% (p > 0.05).
FIG. 1 is a graph of the results of TUNEL staining of cells after treatment with water-soluble tomato concentrate at various concentrations, as described in connection with FIG. 1 and the above results: tomato concentrate at low concentration (250 μg/mL) is not sufficient for hair papilla anti-apoptotic capacity, but combined with its effect of promoting cell proliferation, the overall benefit to cells is greater than bad. While high concentrations (750 μg/mL) of tomato concentrate, while helpful in proliferation of hair papilla cells, also induced apoptosis, affecting the overall life span of the cells. The tomato concentrate with medium concentration (500 mug/mL) has the effects of promoting proliferation and division of cells on hair papilla cells, resisting apoptosis on hair papilla cells, promoting cell growth and prolonging cell life. And the common tomato powder cannot play a remarkable role.
3. Effect of Water-soluble tomato concentrate on dermal papilla cell growth factor expression
3.1 Experimental methods
Three optimal drug concentrations of 250 μg/mL, 500 μg/mL and 750 μg/mL obtained by CCK8 experiments were selected, the experimental group was added with the medium containing the water-soluble tomato concentrate, the blank group was added with the medium containing no drug, after incubation for 48 hours, the cell culture supernatant was taken and ELISA was performed using VEGF (EMC 103.96, neobioscience, china), IGF-1 (EMC 125.96, neobioscience, china), FGF-7 (XY 9M1023, XYBIotechnology, china) and TGF- β1 kit (EMC 107b.96, neobioscience, china).
3.2 experimental results
The standard curve is drawn, taking VEGF as an example, and the results are shown in Table 2 and FIG. 2, wherein the X-axis of the abscissa in FIG. 2 represents the concentration of water-soluble tomato concentrate and the Y-axis of the ordinate represents the OD value.
TABLE 2
Similarly, standard curves for each cytokine can be obtained according to the instructions of the kit, and the factor concentration calculated according to the standard curves, and the results are shown in Table 3.
TABLE 3 Table 3
From the above results, it can be seen that the water-soluble tomato concentrate can increase VEGF (P>0.05）、IGF-1（Represents p<0.05 And significantly reduces TGF- β1 production (+)>Represents p<0.05，/> Representing P<0.01）。
The catagen (catagen) of the hair cycle is an apoptosis-driven process, accompanied by terminal differentiation, proteolysis and matrix remodeling. There are studies showing that VEGF accelerates hair regeneration and increases the size of hair follicles and hair shafts by inducing perifollicular angiogenesis, improving head blood circulation and nutrient supply; IGF-1 is an important factor for regulating hair follicle mitosis and growth differentiation, and the signal transduction pathway has the effects of affecting hair follicle proliferation, tissue remodeling, hair growth cycle conversion and hair follicle differentiation, and up-regulating IGF-1 contributes to the restoration of dermal hair follicle papilla cells and maintains hair induction capacity. Meanwhile, as inhibitors of keratinocyte proliferation and inducers of keratinocyte apoptosis, transforming growth factor beta 1 (TGF-beta 1) has been proposed to play an important role in the regulation of the catagen phase, and the water-soluble tomato concentrate of the present application is capable of significantly reducing TGF-beta 1 production (p < 0.05) in primary papilla, helping to induce or maintain the growth phase of hair follicles, helping to achieve self-remodeling of hair follicles.
The results show that the water-soluble tomato concentrate can obviously promote proliferation of hair papilla cells, inhibit apoptosis of hair papilla cells, especially at 500 mug/mL, promote expression of VEGF and IGF-1 in hair papilla, and reduce expression of TGF-beta 1, and the water-soluble tomato concentrate has the effects of promoting hair follicle to maintain a growing period and inhibiting entering a retrograde period, so that the water-soluble tomato concentrate has good effects of preventing alopecia and promoting hair growth.
4. Effect of Water-soluble tomato concentrate on hair growth in mouse alopecia models
4.1 Experimental methods
Experimental animals: c57BL 6 mice, females, 8 weeks, 15-20g, syngeneic. The skin color pink was selected and the hair was a resting stage mouse. The phase change of the hair period can be judged according to the skin color of the mice, the skin in the hair growth period is black, the retrograde period is gray, and the resting period is pink. Establishing a murine alopecia model: mixing rosin and paraffin at a ratio of 1:1, heating to melt, uniformly coating on the back of a mouse, wherein the coating area is about 3cm multiplied by 3cm, cooling and solidifying, and removing the back hair. The anagen phase is confirmed to be in the telogen phase based on the skin color.
Grouping 8 mice confirmed to be in resting stage; wherein: experimental group: gastric administration of 500mg/mL water-soluble tomato concentrate; positive control group: 2% minoxidil solution; negative control group: purifying the water.
The administration mode is as follows: the medicine is filled into stomach and is administrated 1 time a day, the daily administration amount is 0.15mL, and the medicine is continuously administrated for 21 days.
Results observations and recordings: the skin and hair color changes of the mice are observed visually every day, and the time from the administration to the beginning of blackening of the skin color of the hair removal area at the back of each animal is recorded; the negative control group records the time from day 1 after depilation to the onset of skin darkening in the dehairing area; the dehairing area begins to darken to the time of full hair growth.
4.2 experimental results
As a result, as shown in fig. 3, the mice of the experimental group developed a black local skin on day 5 after depilation, the hair was grown in the anagen phase, the hair was grown after about 10 days, the skin color was changed to pink (telogen phase) again via gray (anagen phase) on day 21, and the skin black and gray remained for about 16 days in total; the positive control mice gradually appear black on local skin on day 5 after dehairing, hair enters anagen phase, new hair grows after 9 days, skin color changes to pink (rest phase) through gray (retrogressive phase) on day 21, and skin black and gray last for 16 days; the local skin of the mice in the negative control group changed from pink to black on the 6 th day after depilation, new hair grew out after 11 days, and the skin color changed from gray to pink again after 20 days, and the skin black and gray lasted for 14 days.
The experiment shows that compared with the mice in the negative control group, the skin color of the mice in the experiment group is changed from pink to black 24 hours in advance, the skin color of the mice in the experiment group is changed from black to gray 24 hours later, the time of the growing period of the hair follicle is about 48 hours longer than that of the mice in the negative control group, the result is similar to that of the positive control group, and the solvent group and the negative control group have no obvious difference, so that the oral water-soluble tomato concentrate can promote the hair follicle to enter the growing period, prolong the growing period and delay the entering the retrograde period.
In conclusion, under the experimental conditions, the water-soluble tomato concentrate has the effects of promoting hair follicles to enter a growing period, prolonging the growing period and delaying entering a withdrawal period for a mouse alopecia model, so that the water-soluble tomato concentrate has good effects of preventing alopecia and promoting hair growth.
The technical features of the above-described embodiments may be arbitrarily combined, and all possible combinations of the technical features in the above-described embodiments are not described for brevity of description, however, as long as there is no contradiction between the combinations of the technical features, they should be considered as the scope of the description.
The above examples merely illustrate a few embodiments of the present application, which are convenient for a specific and detailed understanding of the technical solutions of the present application, but should not be construed as limiting the scope of the claims. It should be noted that it will be apparent to those skilled in the art that several variations and modifications can be made without departing from the spirit of the application, which are all within the scope of the application. It should be understood that, based on the technical solutions provided by the present application, those skilled in the art may obtain technical solutions through logical analysis, reasoning or limited experiments, which are all within the scope of protection of the appended claims. The scope of the patent is therefore intended to be covered by the appended claims, and the description and drawings may be interpreted as illustrative of the contents of the claims.
Claims (5)
1. Use of a water-soluble tomato concentrate for the preparation of an oral product for reducing hair loss and/or hair growth, characterized in that the preparation method of the water-soluble tomato concentrate comprises the steps of:
(1) Diluting cold crushed tomato pulp with browning index less than 0.350AU and brix of 28-30 DEG with ultrapure water, wherein the volume ratio of the cold crushed tomato pulp to ultrapure water is 1:5, centrifuging and clarifying at room temperature by using a Westfalia MSB-14 separator, removing smaller particulate matters by microfiltration at a temperature of not more than 45 ℃ to obtain a clarified pale yellow solution which does not contain insoluble and sedimentation solids and can pass through a 0.2 mu m filter, concentrating the solution into Bai Lidu pulp with temperature of not more than 50 ℃ by evaporation, introducing a rapid pasteurization step with a temperature of 105 ℃ for 3 seconds at the beginning of the evaporation step, and obtaining an intermediate product with browning index less than 0.600AU and total microbial plate count of < 1000;
(2) Preparing 130L of resin column containing FPX66 resin, balancing in ultrapure water at 4 ℃, diluting the intermediate product to have a Brix of 8 DEG, loading the resin column at 260L/min, maintaining the temperature at 4 ℃, once all substances are adsorbed on the resin column, eluting with 130L of washing water, discarding the eluent, then eluting with 130L of hot water at 75 ℃, then eluting with 130L of 80% ethanol, finally eluting with 130L of hot water at 75 ℃, collecting and combining the eluents to obtain 400L of ethanol dilute solution with a concentration of 25%, concentrating the 400L of ethanol dilute solution with a concentration of 25% by reverse osmosis by using Trisep AC5 membrane at 30 ℃, allowing ethanol/water solvent to pass through the membrane, keeping all compounds dissolved in the membrane, once the dilute solution is concentrated to 1/10 of the original volume, namely the volume of the solution is reduced to 40L, beginning to permeate, adding ultrapure water into the retentate at the same rate as the removal rate of the filtered solution, finally gradually reducing the concentration of ethanol dilute solution from 25% to a concentration of <5% by reverse osmosis, and finally obtaining the water-soluble fine powder with a concentration of <6% by spray of water-mist of water-soluble tomato.
2. The use according to claim 1, wherein the product further comprises other substances having an auxiliary effect on hair growth and hair growth, the substances comprising one or more of cowberry fruit extract, grape seed extract, saw palmetto extract, wheat germ extract, semen Cuscutae extract, ginseng extract, chamomile extract, turmeric extract, silybum marianum extract, millet extract, linseed oil, DHA, EPA and vitamin E.
3. The use according to claim 2, wherein the dosage form of the oral product comprises solutions, powders, tablets, granules, capsules, emulsions and suspensions.
4. The use according to claim 3, wherein the product further comprises a health food or a pharmaceutically acceptable adjuvant.
5. The use according to claim 4, wherein the adjuvants comprise one or more of fragrances, preservatives, flavourings, thickeners, binders, disintegrants, lubricants and diluents.
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| CN202311071232.1A CN116803404B (en) | 2023-08-24 | 2023-08-24 | Application of water-soluble tomato concentrate in preparation of hair loss reducing and hair growing products |
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Citations (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR20090012565A (en) * | 2007-07-30 | 2009-02-04 | 대전보건대학 산학협력단 | Hair loss prevention and hair growth promoting composition containing a natural extract |
| JP2009155291A (en) * | 2007-12-27 | 2009-07-16 | Lion Corp | Hair-growing agent for preventing stress-related depilation |
| CN102271697A (en) * | 2008-10-31 | 2011-12-07 | 普罗维斯天然产物有限公司 | Fruit extracts |
| KR101112053B1 (en) * | 2011-06-20 | 2012-02-13 | 신라대학교 산학협력단 | Method for obtaining purified lycopene and water-soluble lycopene derived from tomato |
| CN102641353A (en) * | 2012-05-15 | 2012-08-22 | 何超文 | Hair growth conditioner rich in plant flavonoids |
| KR20130060487A (en) * | 2011-11-30 | 2013-06-10 | 주식회사 에코마인 | Method of extracting lycopene from tomato, lycopene from tomato using the same and cosmetic composition containing the lycopene from tomato for preventing hair loss and promoting hair growth |
| CN107158386A (en) * | 2017-05-31 | 2017-09-15 | 段新方 | Prevent and treat the medicine of alopecia |
| CN110693749A (en) * | 2019-11-05 | 2020-01-17 | 中国中医科学院中药研究所 | Cosmetic composition containing tomato extract and preparation method thereof |
| KR102502664B1 (en) * | 2022-09-23 | 2023-02-23 | 주식회사 에이바이오머티리얼즈 | Cosmetic composition for improving skin and hair condition containing Solanum Lycopersicum(Tomato) Fruit exosome, Brassica Oleracea Italica(Broccoli) exosome |
Family Cites Families (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CA3042720A1 (en) * | 2016-11-02 | 2018-05-11 | Provexis Natural Products Limited | Water soluble tomato extract protects against adverse effects of air pollution |
-
2023
- 2023-08-24 CN CN202311071232.1A patent/CN116803404B/en active Active
Patent Citations (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR20090012565A (en) * | 2007-07-30 | 2009-02-04 | 대전보건대학 산학협력단 | Hair loss prevention and hair growth promoting composition containing a natural extract |
| JP2009155291A (en) * | 2007-12-27 | 2009-07-16 | Lion Corp | Hair-growing agent for preventing stress-related depilation |
| CN102271697A (en) * | 2008-10-31 | 2011-12-07 | 普罗维斯天然产物有限公司 | Fruit extracts |
| KR101112053B1 (en) * | 2011-06-20 | 2012-02-13 | 신라대학교 산학협력단 | Method for obtaining purified lycopene and water-soluble lycopene derived from tomato |
| KR20130060487A (en) * | 2011-11-30 | 2013-06-10 | 주식회사 에코마인 | Method of extracting lycopene from tomato, lycopene from tomato using the same and cosmetic composition containing the lycopene from tomato for preventing hair loss and promoting hair growth |
| CN102641353A (en) * | 2012-05-15 | 2012-08-22 | 何超文 | Hair growth conditioner rich in plant flavonoids |
| CN107158386A (en) * | 2017-05-31 | 2017-09-15 | 段新方 | Prevent and treat the medicine of alopecia |
| CN110693749A (en) * | 2019-11-05 | 2020-01-17 | 中国中医科学院中药研究所 | Cosmetic composition containing tomato extract and preparation method thereof |
| KR102502664B1 (en) * | 2022-09-23 | 2023-02-23 | 주식회사 에이바이오머티리얼즈 | Cosmetic composition for improving skin and hair condition containing Solanum Lycopersicum(Tomato) Fruit exosome, Brassica Oleracea Italica(Broccoli) exosome |
Non-Patent Citations (1)
| Title |
|---|
| 水溶性番茄浓缩物Fruitflow通过调控血小板PI3K/Akt和MAPKs信号通路抑制其活化、聚集及血栓形成;凡蝶;田泽众;马熙麟;左晓;牙甫礼;杨燕;;中山大学学报(医学科学版)(02);243-250 * |
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| CN116803404A (en) | 2023-09-26 |
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