CN116474153B - A skin ulcer care film based on bamboo fiber-low eutectic solvent-gelatin - Google Patents

Abstract

The invention discloses a skin ulcer nursing film based on bamboo fiber-eutectic solvent-gelatin and a preparation method thereof. The bamboo fiber is from the stems of the plants in the bamboo family, gelatin is a film forming agent, and the eutectic solvent consists of choline chloride and ethylene glycol or 1, 2-propylene glycol or glycerol and is used as a bamboo fiber solvent, a film forming auxiliary agent and a penetration enhancer; in addition, bamboo chlorophyll or bamboo charcoal is used as a membrane function object, and a tape casting method is used for preparing the composite adhesive membrane. The preparation process is simple, the cost is low, the film forming property is strong, functional components are convenient to add, and the high-value development and the omnibearing utilization of bamboo resources are realized. The obtained composite film has good indexes such as flexibility, uniformity, color, transparency and the like, the adhesion and elasticity meet the use requirements, and the composite film is easy to attach; also has certain air permeability, moisture absorption and moisture retention. The anti-inflammatory, wound healing, hemostatic and anti-seepage effects of the bamboo chlorophyll and the bamboo charcoal can be cooperated with the anti-ulcer effect of the bamboo fiber, so that the film is multifunctional.

Description

A skin ulcer care film based on bamboo fiber-low eutectic solvent-gelatin

Technical Field

The invention belongs to the technical field of medical devices/medical dressings, and in particular relates to a composite component anti-ulcer skin care film with multiple functions and a preparation method thereof.

Background Art

The skin is the largest organ in the human body, and its main functions are to prevent bacteria and viruses from invading the body, regulate body temperature, and discharge sweat. Skin ulcers are common and frequently occurring surgical diseases, often caused by physical and chemical factors acting directly on tissues or microbial infections. Small-area ulcers are easier to heal on their own, but large-area skin ulcers are often characterized by long-term inability to heal, thus affecting the normal function of the body. The relevant tissues will have varying degrees of defects, liquefaction, and even infection and necrosis, accompanied by symptoms such as itching, pain, ulceration, exudation, and even bleeding. They are prone to protracted healing or recurrence, causing great pain and inconvenience to patients, and seriously affecting their work and quality of life. Therefore, it has become one of the long-standing problems in clinical medicine, and the currently available large-area nursing dressings with more comprehensive functions are very limited.

In recent years, more and more natural products have been found to have a series of effects such as anti-ulcer, anti-inflammatory, inhibiting bacterial growth and improving immunity. Related preparations and dressings have been well applied in clinical practice. At the same time, renewable biomass resources are increasingly valued. For example, new materials based on wood cellulose are widely concerned and researched and developed. Bamboo is a common economic crop in my country with a huge planting area and output. It also shows more and more medical and medicinal value in this field. In folk therapy, bamboo stems are peeled and ground into powder and directly applied to the ulcer surface (about 2-3 mm thick, covered with sterile gauze and fixed with adhesive tape) for treatment (Chinese Folk Therapy, 1999, 000 (005): 47). The bamboo fiber contained in bamboo stems (the average content in moso bamboo is about 22.7%) has natural moisture absorption, breathability and antibacterial properties. When used as a textile material such as underwear, it will not cause any allergic behavior to the skin. These characteristics are of great significance in the treatment of surface ulcers in the clinic, and also provide a basis for the development of more different anti-ulcer topical dressings.

Deep eutectic solvent (DES) is a new multi-purpose green solvent composed of hydrogen bond donor (HBD) and hydrogen bond acceptor (HBA) in a certain molar ratio. It exists in liquid form at room temperature and is currently in its infancy in the field of medicine. Due to its unique physical and chemical properties, it has been found to be able to dissolve a variety of poorly soluble macromolecules represented by cellulose. In addition, it has good biocompatibility and the ability to promote transmission and penetration, making it have great potential in the application direction of external transdermal absorption (Proc Natl Acad Sci USA, 2014, 111 (37): 13313-13318.).

Gelatin is a partial hydrolysis product of collagen. It is widely available, has a three-dimensional network structure that is easy to form, has good biocompatibility with the human body, and has the effect of stopping bleeding, so it is a good film-forming material. However, it has low strength and high brittleness after being dissolved in water. Once swollen, its strength and elastic modulus will decrease rapidly, which greatly limits its application. The introduction of plant fibers can often significantly enhance the flexibility and strength of gelatin. At the same time, the two have good compatibility and stability, and have the potential to be blended and compounded as a matrix for medical materials.

Although bamboo resources are very abundant, there are also many processing by-products from different bamboo-related industries that need to be effectively utilized. For example, bamboo powder in the building materials and furniture industries can be used as an important source of bamboo fiber. Bamboo charcoal generated by pyrolysis of discarded bamboo segments has a porous structure and high porosity. It has strong adsorption capacity and has antibacterial, hemostatic and coagulation effects (Science and Technology Review, 2010(16):113-117). The structure of chlorophyll in bamboo leaves is extremely similar to hemoglobin. It not only has a regenerative effect on skin tissue, but also has anti-inflammatory and wound healing effects (Journal of Northeast Agricultural University, 2009, 40(12):130-134). Using these two substances to treat skin ulcers and injuries can develop more new medical care materials and provide more options for clinical treatment of such diseases.

At present, there are some dressings for treating ulcer-type diseases, most of which are for oral ulcers, and very few are for skin ulcers on the body surface; at the same time, there are major problems such as complex formula and process, limited functions, special equipment required, and gelatin easy to lose water and harden. The present invention provides a composite film with low eutectic solvent, bamboo fiber and gelatin as main components and a preparation method thereof. It has a novel formula, simple preparation, comprehensive functions, and is easy to mass produce. At the same time, it solves the problem of easy dehydration and hardening of the material, and improves the comfort and air permeability of the wound contact; it not only improves the strength, functionality and therapeutic effect of the composite film, but also realizes the high-value development and multiple utilization of bamboo resources.

Summary of the invention

The purpose of the present invention is to provide a method for preparing a composite anti-ulcer skin care film with multiple functions, wherein the basic components involved include a low eutectic solvent, bamboo fiber and gelatin; the preparation process involved is simple, the composite film has comprehensive functions and stable properties, no special equipment is required during preparation, and the film has good development and application prospects. The mixture of the low eutectic solvent and bamboo fiber is compounded in the form of a transparent film using gelatin in a physical manner, which greatly expands the application of existing green solvents in the field of pharmaceutical materials. The good physical and chemical properties of each component in the preparation process reflect obvious combination advantages, and the overall properties, functional integration and mechanical strength of the bamboo fiber-low eutectic solvent-gelatin composite film are better than those of the bamboo fiber-gelatin composite material prepared under the same conditions, and functional materials such as bamboo chlorophyll and bamboo charcoal can be conveniently added to play a synergistic role in anti-ulcer applications. The composite film has a smooth appearance and good uniformity; it is also easy to cut and easy to achieve large-scale preparation. It has strong elasticity and adhesion during use, good thermal stability, air permeability, and moisture absorption and moisture retention properties, and also reflects the characteristics of being able to be used as a drug release carrier.

Technical solution: In order to achieve the above purpose, a preparation method of a composite multifunctional skin ulcer care film is proposed:

The composite multifunctional skin ulcer care film of the present invention is characterized in that the low eutectic solvent used as the bamboo fiber solvent, film-forming aid and penetration promoter includes but is not limited to one of choline chloride-ethylene glycol (1:2), choline chloride-1,2-propylene glycol (1:2) or choline chloride-glycerol (1:2).

The method for preparing the composite multifunctional skin ulcer care film is characterized in that the film material used is bamboo fiber.

The method for preparing the composite multifunctional skin ulcer care film is characterized in that the film-forming agent used is gelatin.

The specific preparation steps are as follows:

(1) Mix bamboo fiber and low eutectic solvent in a mass ratio of 1:10 to 1:15, heat at 110° C. for 1 to 2 hours to form a homogeneous solution; then add a saturated gelatin aqueous solution of 5 to 25 times the mass of the bamboo fiber, stir at 80° C. for 0.5 to 1 hour to prepare a membrane slurry;

(2) When a functional substance is to be added, chlorophyll or bamboo charcoal is added in a mass ratio of functional substance to membrane slurry of 5:100 to 15:100, and then stirred at room temperature to be evenly dispersed;

The functional substance in step (2) includes but is not limited to bamboo chlorophyll and bamboo charcoal;

(3) continuously evaporating the uniformly mixed system formed in (1) or (2) under vacuum conditions to remove water until the weight stops decreasing;

(4) Defoaming: decompression and degassing treatment in a vacuum drying environment;

(5) Film coating: Pour all the film slurry onto a smooth and clean glass surface, wait for it to flow naturally and control the film to form a 1-2 mm thick film;

(6) Standing and removing the film: Let the prepared film stand naturally for 24 to 48 hours, and remove the film after it is completely cured;

(7) Packaging: Cut the prepared film into certain sizes, sterilize it under ultraviolet light for 60 minutes, and then pack it and seal it for storage.

In the above-mentioned composite multifunctional skin ulcer care film preparation method, the film thickness range includes but is not limited to 1 to 2 mm; the thickness of the prepared film can be adjusted by controlling the film slurry volume according to actual needs. For example, the obtained film thickness can be 1.0 mm, 1.2 mm, 1.4 mm, 1.8 mm, 2.0 mm, etc.

BRIEF DESCRIPTION OF THE DRAWINGS

In order to more completely illustrate the technical solution of the specific embodiment of the present invention, the following briefly describes the drawings required in the embodiment. The drawings described are only part of the embodiment of the present invention. For those skilled in the art, it should be possible to obtain other drawings of the embodiment based on these drawings. Among them:

FIG1 is an appearance of a base film of a bamboo fiber-deep eutectic solvent-gelatin composite film (without adding functional substances) in the present invention;

FIG2 is an optical microscope image of the bamboo fiber-deep eutectic solvent-gelatin composite film of the present invention;

FIG3 is an atomic force microscopic phase diagram (left) and a three-dimensional diagram (right) of the bamboo fiber-deep eutectic solvent-gelatin composite film of the present invention;

FIG4 is a front view of a scanning electron microscope of a bamboo fiber-deep eutectic solvent-gelatin composite film of the present invention;

FIG5 is an infrared spectrum (FT-IR) diagram of the bamboo fiber-deep eutectic solvent-gelatin composite film of the present invention (a is bamboo chlorophyll; b is bamboo charcoal; c is a composite film with bamboo chlorophyll added; d is a composite film with bamboo charcoal added);

FIG6 is an air permeability experiment of the bamboo fiber-deep eutectic solvent-gelatin composite film of the present invention (a is a state where the gel film is tightly fitted to the funnel; b is an overall diagram of the air permeability experimental device; c is a phenomenon in which bubbles are generated in water after air flows through the film);

FIG. 7 shows the moisture absorption and moisture retention test results of the bamboo fiber-deep eutectic solvent-gelatin composite film of the present invention (a and b are moisture absorption curves under humidity of 43% and 81% respectively; c is the moisture retention curve under humidity of 0%).

DETAILED DESCRIPTION

The following is a detailed description of the present invention. Although specific embodiments of the present invention are shown, it should be understood that the present invention can be implemented in various forms and should not be limited by the embodiments set forth herein. On the contrary, these embodiments are provided to enable a more thorough understanding of the present invention and to fully convey the scope of the present invention to those skilled in the art.

This specification and claims do not use differences in terms as a way to distinguish components, but use differences in the functions of the components as the criteria for distinction. The "include" or "comprising" mentioned in this application is an open-ended term and should be interpreted as "including but not limited to". The embodiments described in the subsequent description are preferred embodiments of the present invention and are based on the general principles of the specification and are not intended to limit the scope of the present invention.

Example 1

Weigh 0.3g of bamboo fiber and 3.0g of low eutectic solvent, mix them, heat at 110°C for 1 hour to form a homogeneous solution; then add 7.5g of saturated gelatin aqueous solution, stir at 80°C for 0.5 hour to obtain a membrane slurry; the mass ratio of the bamboo fiber to the low eutectic solvent is 1:10, and the mass ratio of the saturated gelatin aqueous solution to the bamboo fiber powder is 25:1;

The membrane slurry is continuously evaporated to remove water under vacuum conditions until the weight stops decreasing, and then subjected to a decompression degassing treatment;

Pour the degassed membrane slurry onto a smooth and clean glass surface and wait for it to flow naturally. After standing naturally for 24 hours and completely solidifying, peel off the membrane to obtain a bamboo fiber-low eutectic solvent-gelatin composite membrane; after sterilization by ultraviolet light for 60 minutes, punch, package and seal for storage.

Example 2

Weigh 0.1g of bamboo fiber and 1.5g of a low eutectic solvent, mix them, and heat them at 110°C for 1 hour to form a homogeneous solution; then add 2.0g of a saturated aqueous solution of gelatin, and stir at 80°C for 1 hour to obtain a membrane slurry; the mass ratio of the bamboo fiber to the low eutectic solvent is 1:15, and the mass ratio of the saturated aqueous solution of gelatin to the bamboo fiber powder is 25:1;

The membrane slurry is continuously evaporated to remove water under vacuum conditions until the weight stops decreasing, and then subjected to a decompression degassing treatment;

Pour the degassed membrane slurry onto a smooth and clean glass surface and wait for it to flow naturally. After standing naturally for 24 hours and completely solidifying, peel off the membrane to obtain a bamboo fiber-low eutectic solvent-gelatin composite membrane; after sterilization by ultraviolet light for 60 minutes, punch, package and seal for storage.

Example 3

Weigh 0.4 g of bamboo fiber and 5.0 g of a low eutectic solvent, mix them, and heat them at 110° C. for 1 hour to form a homogeneous solution; then add 2.5 g of a saturated gelatin aqueous solution, and stir at 80° C. for 1 hour to obtain a membrane slurry; the mass ratio of the bamboo fiber to the low eutectic solvent is 1:12.5, and the mass ratio of the saturated gelatin aqueous solution to the bamboo fiber powder is 6.25:1;

The membrane slurry is continuously evaporated to remove water under vacuum conditions until the weight stops decreasing, and then subjected to a decompression degassing treatment;

Pour the degassed membrane slurry onto a smooth and clean glass surface and wait for it to flow naturally. After standing naturally for 24 hours and completely solidifying, peel off the membrane to obtain a bamboo fiber-low eutectic solvent-gelatin composite membrane; after sterilization by ultraviolet light for 60 minutes, punch, package and seal for storage.

Example 4

Weigh 0.5 g of bamboo fiber and 6.0 g of a low eutectic solvent, mix them, and heat them at 110° C. for 1 hour to form a homogeneous solution; then add 10.0 g of a saturated gelatin aqueous solution, and stir at 80° C. for 1 hour to obtain a membrane slurry; the mass ratio of the bamboo fiber to the low eutectic solvent is 1:12, and the mass ratio of the saturated gelatin aqueous solution to the bamboo fiber powder is 20:1;

The membrane slurry is continuously evaporated to remove water under vacuum conditions until the weight stops decreasing, and then subjected to a decompression degassing treatment;

Pour the degassed membrane slurry onto a smooth and clean glass surface and wait for it to flow naturally. After standing naturally for 24 hours and completely solidifying, peel off the membrane to obtain a bamboo fiber-low eutectic solvent-gelatin composite membrane; after sterilization by ultraviolet light for 60 minutes, punch, package and seal for storage.

Example 5

Weigh 0.2g of bamboo fiber and 2.8g of low eutectic solvent, mix them, heat at 110°C for 1 hour to form a homogeneous solution; then add 3.0g of saturated gelatin aqueous solution, stir at 80°C for 1 hour to obtain a membrane slurry; the mass ratio of the bamboo fiber to the low eutectic solvent is 1:14, and the mass ratio of the saturated gelatin aqueous solution to the bamboo fiber powder is 15:1;

The membrane slurry is continuously evaporated to remove water under vacuum conditions until the weight stops decreasing, and then subjected to a decompression degassing treatment;

Pour the degassed membrane slurry onto a smooth and clean glass surface and wait for it to flow naturally. After standing naturally for 24 hours and completely solidifying, peel off the membrane to obtain a bamboo fiber-low eutectic solvent-gelatin composite membrane; after sterilization by ultraviolet light for 60 minutes, punch, package and seal for storage.

Example 6

Weigh 0.4 g of bamboo fiber and 5.6 g of a low eutectic solvent, mix them, and heat them at 110° C. for 1 hour to form a homogeneous solution A; then add 3.0 g of a saturated gelatin aqueous solution, and stir at 80° C. for 0.5 hour to obtain a membrane slurry; the mass ratio of the bamboo fiber to the low eutectic solvent is 1:14, and the mass ratio of the saturated gelatin aqueous solution to the bamboo fiber powder is 7.5:1;

The membrane slurry is continuously evaporated to remove water under vacuum conditions until the weight stops decreasing, and then subjected to a decompression degassing treatment;

Pour the degassed membrane slurry onto a smooth and clean glass surface and wait for it to flow naturally. After standing naturally for 24 hours and completely solidifying, peel off the membrane to obtain a bamboo fiber-low eutectic solvent-gelatin composite membrane; after sterilization by ultraviolet light for 60 minutes, punch, package and seal for storage.

Example 7

Weigh 0.4 g of bamboo fiber and 5.2 g of a low eutectic solvent, mix them, and heat them at 110° C. for 1 hour to form a homogeneous solution; then add 3.5 g of a saturated gelatin aqueous solution, and stir at 80° C. for 1 hour to obtain a membrane slurry; the mass ratio of the bamboo fiber to the low eutectic solvent is 1:13, and the mass ratio of the saturated gelatin aqueous solution to the bamboo fiber powder is 8.75:1;

The membrane slurry is continuously evaporated to remove water under vacuum conditions until the weight stops decreasing, and then subjected to a decompression degassing treatment;

Pour the degassed membrane slurry onto a smooth and clean glass surface and wait for it to flow naturally. After standing naturally for 24 hours and completely solidifying, peel off the membrane to obtain a bamboo fiber-low eutectic solvent-gelatin composite membrane; after sterilization by ultraviolet light for 60 minutes, punch, package and seal for storage.

Example 8

Weigh 0.4 g of bamboo fiber and 4.4 g of a low eutectic solvent, mix them, and heat them at 110° C. for 1 hour to form a homogeneous solution; then add 10.0 g of a saturated gelatin aqueous solution, and stir at 80° C. for 1 hour to obtain a membrane slurry; the mass ratio of the bamboo fiber to the low eutectic solvent is 1:11, and the mass ratio of the saturated gelatin aqueous solution to the bamboo fiber powder is 25:1;

The membrane slurry is continuously evaporated under vacuum conditions to remove water until the weight stops decreasing;

Bamboo chlorophyll was weighed according to the mass ratio of bamboo chlorophyll: film slurry = 15:100, added to the film slurry after water removal, fully stirred at room temperature to make it evenly dispersed, and then decompressed and degassed;

Pour the degassed membrane slurry onto a smooth and clean glass surface and wait for it to flow naturally. After standing naturally for 24 hours and completely solidifying, peel off the membrane to obtain a bamboo fiber-low eutectic solvent-gelatin composite membrane loaded with bamboo chlorophyll; after sterilization by ultraviolet light for 60 minutes, punch, package and seal for storage.

Embodiment 9

Weigh 0.8g of bamboo fiber and 10.0g of low eutectic solvent, mix them, heat at 110°C for 1.5 hours to form a homogeneous solution; then add 4.8g of saturated gelatin aqueous solution, stir at 80°C for 1 hour to obtain a membrane slurry; the mass ratio of the bamboo fiber to the low eutectic solvent is 1:12.5, and the mass ratio of the saturated gelatin aqueous solution to the bamboo fiber powder is 6:1;

The membrane slurry is continuously evaporated to remove water under vacuum conditions until the weight stops decreasing, and then subjected to a decompression degassing treatment;

Bamboo chlorophyll was weighed according to the mass ratio of bamboo charcoal: film pulp = 10:100, added to the film pulp after water removal, fully stirred at room temperature to make it evenly dispersed, and then decompressed and degassed;

Pour the degassed membrane slurry onto a smooth and clean glass surface and wait for it to flow naturally. After standing naturally for 24 hours and completely solidifying, peel off the membrane to obtain a bamboo fiber-low eutectic solvent-gelatin composite membrane loaded with bamboo charcoal; after sterilization by ultraviolet light irradiation for 60 minutes, punch, package and seal for storage.

The functional material-loaded bamboo fiber-deep eutectic solvent-gelatin composite films prepared in Examples 8 to 9 were tested by infrared spectroscopy.

The infrared test results are shown in Figure 5. The following is a brief analysis of the infrared test results:

The upper figure shows the FT-IR spectra of bamboo chlorophyll (a) and gelatin film loaded with bamboo chlorophyll (c), and the lower figure shows the FT-IR spectra of bamboo charcoal (b) and gelatin film loaded with bamboo charcoal (d). In the typical signals of the chlorophyll spectrum, the absorption peaks near 1600 and 1276 cm ^-1 are generated by the C=C coupling vibration on the porphyrin ring and the stretching vibration of CN. In the spectrum of the composite film with chlorophyll added, relevant signals also appear at the corresponding wavenumbers, indicating that bamboo chlorophyll has been successfully loaded. Similarly, in the typical characteristic signals of the bamboo charcoal spectrum, the absorption peaks near 1650 and 1176 cm ^-1 are generated by the vibration of the aromatic ring skeleton and the stretching vibration of CO, respectively. Relevant signals can also be observed at the corresponding wavenumbers of the composite film spectrum with bamboo charcoal added, indicating that the membrane has been successfully loaded with bamboo charcoal.

The air permeability of the bamboo fiber-deep eutectic solvent-gelatin composite membrane loaded with functional substances prepared in Examples 8 to 9 was tested. A corresponding simple device was built based on the national standards GB/T228192008 and GBT7755.1-2018, and the air permeability was judged by observing whether bubbles were generated in the liquid layer at the tail end of the device after the air flow passed through the film. The specific results are shown in Figure 6.

The moisture absorption and moisture retention performance of the bamboo fiber-deep eutectic solvent-gelatin composite film loaded with functional substances prepared in Examples 8 to 9 were tested. The results are shown in Figure 7. The specific operation is as follows:

Moisture absorption experiment: 0.5g of the composite film was accurately weighed and placed in a wide-mouth weighing bottle with a diameter of 4cm, and then placed in a desiccator filled with a saturated aqueous solution of potassium carbonate (relative humidity was controlled as needed), with the overall temperature kept constant at 20°C. The moisture absorption rate was calculated as _Ra = 100*( _Wn - _Wo )/ _Wo ; where _Wo and _Wn were the masses of the composite film before and after 48h of placement.

Moisture retention experiment: 0.5g of composite film containing 10% water was accurately weighed and placed in a wide-mouth weighing bottle with a diameter of 4cm, and then placed in a desiccator pre-filled with silica gel, with the overall temperature kept constant at 20°C. The moisture retention rate (Rh%) was calculated as Rh% = 100* _Hn / _H0 ; where _H0 and _Hn are the mass of water in the composite film before and after 48h.

The overall properties and adhesion of the bamboo fiber-deep eutectic solvent-gelatin composite films described in Examples 1 to 9 were compared. The specific operation of the adhesion test is as follows:

Take two pieces of thermoplastic elastomer synthetic rubber (1cm*3cm, weighing about 5g), one of which is connected to the lower end of the tray on one side of the balance, and the other is fixed to the base of the balance. Then place the film (1cm*3cm) between the two pieces of rubber, press it tightly with a 50g weight for 5 seconds (in line with the pressing time of common patches), and then remove the weight; then continue to add weights to the tray on the other side of the balance to increase the gravity, and use the separation of the upper and lower pieces of rubber within 5 seconds as the standard. The mass of the added weight is used to measure the adhesion of the film. The test results are shown in Table 1.

Table 1, comparison results of overall properties and adhesion of bamboo fiber-deep eutectic solvent-gelatin composite films obtained in different embodiments:

Serial number Overall characteristics Adhesion force (g) Example 1 Good uniformity, good flexibility, good film-forming properties 38.6 Example 2 Good uniformity, good flexibility, good film-forming properties 32.5 Example 3 Good uniformity, good flexibility, good film-forming properties 33.1 Example 4 Poor uniformity, good flexibility, average film-forming properties 15.5 Example 5 Good uniformity, good flexibility, good film-forming properties 30.5 Example 6 Good uniformity, good flexibility, average film-forming properties 31.9 Example 7 Good uniformity, good flexibility, average film-forming properties 35.0 Example 8 Good uniformity, good flexibility, good film-forming properties 36.7 Embodiment 9 Good uniformity, poor flexibility, good film-forming properties 35.5

The chlorophyll accumulation permeability of the bamboo fiber-low eutectic solvent-gelatin composite film added with bamboo chlorophyll prepared in Example 8 was measured. The test conditions and results are shown in Table 2.

Table 2, conditions and results of the measurement of the accumulated permeation amount of the bamboo fiber-deep eutectic solvent-gelatin composite membrane loaded with bamboo chlorophyll through the polydimethylsiloxane (PDMS) membrane:

Claims (4)

1. The composite multifunctional skin ulcer nursing film is characterized by comprising a film material, a film forming agent, a eutectic solvent and an additive functional substance, wherein the film material is bamboo fiber, and the film forming agent is gelatin.

2. The composite multifunctional skin ulcer care patch of claim 1, wherein the eutectic solvent is choline chloride-ethylene glycol, choline chloride-1, 2-propanediol or choline chloride-glycerol, and the ratio of the two components is 1:2.

3. The composite multifunctional skin ulcer care patch of claim 1, wherein the addable functional substance is bamboo charcoal or bamboo chlorophyll.

4. The composite multifunctional skin ulcer nursing pad pasting of claim 1, wherein, after simple physical mixing, bamboo fiber and eutectic solvent are uniformly stirred together with gelatin solution, and then the composite membrane is prepared through the steps of water removal, defoaming, film coating and the like, the specific steps are as follows:

(1) Mixing bamboo fibers and eutectic solvent in a mass ratio of 1:10-1:15, and heating at 110 ℃ for 1-2 hours to form a homogeneous solution; adding gelatin saturated water solution with the mass of 5-25 times of that of bamboo fibers, and stirring at 80 ℃ for 0.5-1 hour to prepare membrane slurry;

(2) When the functional substance is required to be added, adding bamboo chlorophyll or bamboo charcoal according to the mass ratio of the functional substance to the membrane slurry=5:100-15:100, and fully stirring at room temperature to uniformly disperse the functional substance;

(3) Evaporating the uniform mixed system formed in the step (1) or the step (2) under vacuum to remove water until the weight is no longer reduced;

(4) Defoaming: vacuum defoaming treatment is carried out in a vacuum drying environment;

(5) And (3) coating: pouring all the film slurry on a smooth and clean glass plane, and forming a thick film with the thickness of 1-2 mm after the film slurry naturally flows in a casting way;

(6) Standing and membrane opening: naturally standing the prepared film for 24-48 hours, and uncovering the film after complete solidification;

(7) And (5) subpackaging: cutting the prepared composite film into a certain specification, irradiating with ultraviolet lamp for 60min for sterilization, packaging, and sealing for preservation.