CN202920687U - Biological inductive active dressing for skin wound and medical dressing compound - Google Patents
Biological inductive active dressing for skin wound and medical dressing compound Download PDFInfo
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- CN202920687U CN202920687U CN 201220205537 CN201220205537U CN202920687U CN 202920687 U CN202920687 U CN 202920687U CN 201220205537 CN201220205537 CN 201220205537 CN 201220205537 U CN201220205537 U CN 201220205537U CN 202920687 U CN202920687 U CN 202920687U
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Abstract
The utility model provides a medical dressing for protecting a skin wound and accelerating repair. The medical dressing comprises a biological inductive active dressing for the skin wound and a medical polyurethane adhesive film, wherein the biological inductive active dressing for the skin wound comprises a framework materials synthesized by a polyanion substance and a polycation substance; polypeptide and bonding polypeptide and hyaluronic acid are distributed in the hole of the framework material and/or polypeptide and bonding polypeptide and hyaluronic acid molecules are connected to the molecules of the framework material; and the pH value of the biological inductive active dressing for the skin wound is 5.8-6.4. The medical dressing for protecting a skin wound and accelerating repair provided by the utility model not only has the advantage of a wound coverage medical dressing, but also has the synergistic effect of bonding polypeptide and hyaluronic acid, so that the structural design of the medical dressing material is optimized without additional growth factors or living cells. The medical dressing material is endowed with the function of in-situ inducing tissue regeneration and repair.
Description
Technical field
This utility model relates to a kind of medical dressing, particularly relates to a kind of for skin wound protection, the medical dressing and the application thereof that promote reparation and the method for preparing this medical dressing.
Background technology
The wound surface of present clinical use covers dressing and mainly comprises traditional dressing, natural biological dressing, synthesising biological dressing three major types.Traditional dressing such as gauze, cotton pad etc. are the dressing of commonly using clinically, its advantage is: the protection wound surface, water absorption arranged, make simple, low price, but exist can't keep that wound surface is moistening and wound healing postpones, the dressing fiber easily comes off cause the wound surface foreign body reaction affect wound healing, wound surface granulation tissue easily grow into easy damaged when changing dressings in the mesh of gauze and affect wound healing and cause pain, pathogen easily by the dressing of infiltration cause traumatic infection, the workload of changing dressings greatly and the birth defect that wound healing is difficult to overcome without facilitation etc.; Natural biological dressing has from body skin, alloskin, radiated pig skin, amniotic membrane etc.At present the optimal method of flap coverage is the autologous transplanting skin, but to the extensive wound patient, and wretched insufficiency seems from body skin source; The permeability of alloskin is similar to patient's skin with adhesiveness, can effectively reduce antigenicity, reduces rejection, and price is high, the source is limited, preservation condition requires high clinical problem and ethnics Problem but exist; Although xenogenesis skin dressing source is relatively extensive, cheap, has the problems such as antigenicity, zest, viral infection, and is not suitable for suitability for industrialized production; Synthesising biological dressing mainly contains the dressing of chitin (or chitosan) class, the dressing of Sargassum acids, the dressing of fibroin albumen class, the dressing of Bacterial cellulose class, the dressing of collagen class now.The dressing of chitin (or chitosan) class has that histocompatibility is good, hemostatic function is good, anti-infection ability is strong, but adhesiveness and compliance are all relatively poor; Sargassum acids dressing moisture pick-up properties is high, can keep moistening environment of wound surface, cost is low, causes that wound surface damages again but easily harden; The dressing of fibroin albumen class has good cell and tissue affinity, oxygen flow permeability good, but its water absorption, toughness, mechanicalness and anti-microbial property have much room for improvement; Bacterial cellulose class dressing non-immunogenicity, good water-permeable and air permeable, strong with wound surface close adhesion, mechanical strength and plasticity come large-scale low-cost ground production but lack an effective fermentation system; Collagen class dressing good biocompatibility has the hemostasis coagulant, promotes the cell division differentiation, but stability is more weak, poor flexibility, matter are crisp, not water-fast, and derives from animal, has the danger of infection.Biological dressing development rapidly, various biological dressings continue to bring out, although various biological dressing has good performance in one aspect, all have defective more or less, do not have a kind of cheap, all very good dressing of various performances.At present, mainly contain three directions in the research of biological dressing: can improve wound healing with the material that reduces cicatrix by healing acceleration by adding; Work out performance as the skin substituent from the body skin; Research can promote the associated protein of skin healing.By building a kind of composite double layer dressing, different efficacies is given full play in inside and outside two-layer dressing: outer dressing can prevent that body fluid runs off, controls the moisture evaporation, anti-bacteria infects; Internal layer dressing promotes adhesion, the promotion tissue growth to wound surface, thereby makes up the deficiency of single dressing, is the present the latest development trend of biological dressing.
The utility model content
The purpose of this utility model is to provide active dressing of a kind of skin wound biotic induce and its preparation method and application, is used for solving problems of the prior art, and this dressing is that a kind of comfortableness is good and can promote the functional dressing of skin trauma reparation.
Reach to achieve these goals other relevant purposes; at first this utility model provides a kind of biological pluralgel film dressing; and then provide a kind of skin wound biotic induce active dressing, namely a kind of for the skin wound protection, promote the medical dressing complex that skin wound is repaired.
A kind of biological pluralgel film dressing, comprise the framework material that polyanionic materials and range of polycationic substances are synthetic, be furnished with in the hole of described framework material on the molecule of polypeptide and bonding polypeptide and hyaluronic acid and/or framework material and be connected with polypeptide and bonding polypeptide and hyaluronan molecule.
Better, described biological pluralgel film dressing is transparent membranaceous or sponge is membranaceous.
Further, the synthetic framework material of described polyanionic materials and range of polycationic substances is space network.
A kind of medical dressing complex for skin wound protection, the reparation of promotion skin wound; comprise the biological pluralgel film dressing that can directly contact skin wound that is sealed in packaging bag and away from the medical polyurethane adhesive film of skin wound, the both sides of described biological pluralgel film dressing also are coated with the PE film.
Better, described biological pluralgel film dressing, comprise the framework material that polyanionic materials and range of polycationic substances are synthetic, be furnished with in the hole of described framework material on the molecule of polypeptide and bonding polypeptide and hyaluronic acid and/or framework material and be connected with polypeptide and bonding polypeptide and hyaluronan molecule.
Better, described biological pluralgel film dressing is transparent membranaceous or sponge is membranaceous.
Better, put adjacent with the medical polyurethane adhesive film of biological pluralgel film dressing in described packaging bag.
Further, the synthetic framework material of described polyanionic materials and range of polycationic substances is space network.
Better, described packaging bag can be adopted aluminium plastic bag.
Medical dressing complex for skin wound protection, the reparation of promotion skin wound of the present utility model; in use; biological pluralgel film dressing directly contacts skin wound as internal layer; and the medical polyurethane adhesive film is placed in the skin of biological pluralgel film dressing; directly do not contact with skin wound; biological pluralgel film dressing and the outer field medical polyurethane adhesive film of internal layer are used in conjunction with; be convenient to medical personnel and carry out cutting according to the wound surface of difformity, different parts, have sliceable property.
Medical dressing complex for skin wound protection, the reparation of promotion skin wound of the present utility model comprises biological pluralgel film dressing and medical polyurethane adhesive film; Described biological pluralgel film dressing comprises following raw material components: framework material, hyaluronic acid, polypeptide and bonding polypeptide that polyanionic materials and range of polycationic substances are synthetic; Wherein, in the framework material that described polyanionic materials and range of polycationic substances are synthesized, the mass percent of range of polycationic substances is 60-80%, and the mass percent of polyanionic materials is 20-40%; In described biological pluralgel film dressing, the quality percentage composition that polypeptide and bonding polypeptide account for described framework material is 5-60%, and the quality percentage composition that hyaluronic acid accounts for described biological pluralgel film dressing soluble solids is 0.02-1%; The pH value of described biological pluralgel film dressing is 5.8-6.4, and namely pH value is 6.1 ± 0.3.
Better, described hyaluronic viscosity-average molecular weight is less than 500,000, and preferred viscosity-average molecular weight is 10000.Molecular weight plays the promotion angeogenesis less than 500,000 hyaluronic acid, and has the effect of moisturizing.
Better, described polypeptide and bonding polypeptide one or more polypeptide and bonding polypeptide that separation and Extraction purification obtains after hydrolysis or enzymolysis for collagen, fibronectin, vitronectin, laminin (also claiming laminin，LN), platelet being combined in albumen and fibroin albumen; Be preferably fish skin collagen the separation and Extraction purification obtains after hydrolysis or enzymolysis polypeptide and bonding polypeptide.The bonding polypeptide plays a part short cell adhesion, propagation, and has reduced the immunogenicity of large molecular weight protein, and the while polypeptide has good moisture retention and increases the pliable and tough and compliance of film.The method of said hydrolyzed or enzymolysis can adopt hydrolysis or enzyme solution commonly used in prior art.
Preferably, in described biological pluralgel film dressing, the quality percentage composition that polypeptide and bonding polypeptide account for described framework material is 10-50%.
Preferably, the viscosity-average molecular weight of described polypeptide and bonding polypeptide is less than 3000.
The sequence of bonding polypeptide described in the utility model (being the cell adhesion recognition sequence) is for containing RGDS, LDV, REDVs, RGDV, LRGDN, IKVAV, YIGSR, PDSGR, RYVVLPR, LGTIPG, RGD, LRE, RNIAEIIKDA, VTXG, DGEAs, the polypeptide of GPIbs aminoacid sequence.
Better, described polyanionic materials is polyanionic polysaccharide or polyanion polypeptide, comprises carboxymethyl chitosan, alginic acid (or alginate), collagen, hyaluronic acid, carboxymethyl cellulose, carboxymethyl starch, polyglutamic acid.
Preferably, described polyanionic materials is selected from carboxymethyl chitosan or/and polyglutamic acid.
Hyaluronic acid in polyanionic materials of the present utility model is selected from viscosity-average molecular weight greater than 500,000 hyaluronic acid.
Better, described range of polycationic substances is polycation polysaccharide or polycation polypeptide, comprises chitosan, polylysine.
Preferably, described range of polycationic substances is selected from chitosan.
Range of polycationic substances of the present utility model is mixed with certain proportion the cross-linked network that polyelectrolyte effect formation occurs with polyanionic materials and is guaranteed that framework material has suitable mechanical property and pliability, can improve the comfortableness of this medical dressing; Guarantee simultaneously this medical dressing with appropriate positive charge with play antibiotic and the hemostasis effect.
The pH value of biological pluralgel film dressing of the present utility model remains the faintly acid of 5.8-6.4, skin wound is played promote healing and bacteriostasis.
This utility model adopts the moisture-keeping function of polyglutamic acid, hyaluronic acid and polypeptide and has guaranteed that with the combination of outer polyurethane adhesive film this medical dressing has good moisture absorption, breathability, and has kept the required environment of skin wound " moist reparation ".
Another purpose of the present utility model is to provide a kind of for skin wound protection, the preparation method that promotes the medical dressing complex that skin wound is repaired and the preparation method of above-mentioned biological pluralgel film dressing.
A kind of preparation method of protecting, promote the medical dressing complex that skin wound is repaired for skin wound; comprise the preparation of biological pluralgel film dressing, described for skin wound protection, promote the preparation of the medical dressing complex of skin wound reparation specifically to comprise the steps:
(1) proportioning according to each raw material in biological pluralgel film dressing is dissolved in polyanionic materials in water for injection, and stirring at normal temperature adds glycerol to the polyanionic materials dissolve complete, and continues to stir, and obtains the solution of polyanionic materials;
(2) range of polycationic substances is added in the aqueous acid of solubilized range of polycationic substances according to the proportioning of each raw material in biological pluralgel film dressing or in water for injection, stirring at normal temperature obtains the solution of range of polycationic substances to being uniformly dissolved.
(3) proportioning according to each raw material in biological pluralgel film dressing is dissolved in polypeptide and bonding polypeptide in water for injection, and stirring at normal temperature is the rear solution that obtains polypeptide and bonding polypeptide evenly.
(4) proportioning according to each raw material in biological pluralgel film dressing adds hyaluronic acid in water for injection, and stirring at normal temperature is the hyaluronic solution of rear acquisition evenly.
(5) solution and the hyaluronic solution with solution, polypeptide and the bonding polypeptide of the solution of the polyanionic materials that obtains, range of polycationic substances carries out respectively high temperature sterilize.
(6) first with solution and the hyaluronic solution stirring mix homogeneously of solution, polypeptide and the bonding polypeptide of the polyanionic materials after autoclaving, obtain mixed solution A, solution with the range of polycationic substances after autoclaving is added dropwise in this mixed solution again, and stir while dripping, obtain mixed solution B, and the adjusting pH value is 5.8-6.4.
(7) mixed solution B is poured in stainless steel disc, curtain coating is even, is dried to gel film, is described biological pluralgel film dressing.
(8) gel film is cut to required specification, adopts the PE film to cover on the gel film both sides after cutting, and pack together with the medical polyurethane adhesive film of dimension, and sterilization treatment gets final product.
Better, in step (1), in the solution of described polyanionic materials, the concentration of polyanionic materials is 0.005-0.05g/ml; Better, the volume ratio of glycerol and water for injection is 1:(30-150); Preferably, the volume ratio of glycerol and water for injection is 1:(50-100).
Better, in step (1), the time of described stirring at normal temperature is as 1h; The time that described continuation is stirred is as 1h.
Better, in step (2), in the solution of described range of polycationic substances, the concentration of range of polycationic substances is 0.005-0.1g/ml.
Better, in step (2), the acid of described solubilized range of polycationic substances is as lactic acid, acetic acid, hydrochloric acid, citric acid or phosphoric acid; In step (2), in the aqueous acid of described solubilized range of polycationic substances, to be controlled to be the pH value of this aqueous acid be 1.0-4.0 to the addition of the acid of solubilized range of polycationic substances.
Better, in step (2), the time of described stirring at normal temperature, as be 4h.
Better, in step (3), in the solution of described polypeptide and bonding polypeptide, the concentration of polypeptide and bonding polypeptide is 0.005-0.1g/ml.
Better, in step (4), in described hyaluronic solution, hyaluronic concentration is 0.0002-0.002g/ml.
Better, in step (4), the time of the stirring of described room temperature, as be 1h.
Better, in step (5), the temperature of described high temperature is 100-130 ° of C; The time of described high temperature sterilize was as 30 minutes; Described high temperature sterilize can be placed in pressure cooker and carry out.
Better, in step (6), the mode that slowly drips is adopted in described dropping, speed that this slowly drips, as can drip for 10-60/minute.
Better, in step (7), the mode of described drying is preferably oven dry, natural drying and lyophilization, and oven dry and natural drying can guarantee the light transmission of this biology pluralgel film dressing, can observe directly the situation of wound;
Better, in step (7), the dry rear gel film that obtains also can be further crosslinked; Its crosslinked method is: the gel film of drying is put into the aqueous solution of water-soluble carbodiimide and N-hydroxy-succinamide or the aqueous solution of water-soluble carbodiimide and N-hydroxy thiosuccinimide, stirring reaction 15-25 hour, rinse to soak with distilled water and remove uncrosslinked composition, oven dry or lyophilization obtain hyaline membrane or sponge membrane.The mass body volume concentrations of the water-soluble carbodiimide in the aqueous solution of above-mentioned water-soluble carbodiimide and N-hydroxy-succinamide and N-hydroxy-succinamide is 1-3.5mg/ml.Above-mentioned water-soluble carbodiimide comprises the soluble derivative of 1-ethyl-(3-dimethylaminopropyl) carbodiimide hydrochloride or other carbodiimide.
Better, in step (8), the mode of described sterilization adopts irradiation sterilization or ethylene oxide sterilizing; Described package encapsulation can adopt aluminium plastic bag to carry out package encapsulation.
Biological plural gel dressing of the present utility model contacts with skin wound as internal layer, and is used in conjunction with outer field medical polyurethane adhesive film, is convenient to medical personnel and carries out cutting according to the wound surface of difformity, different parts, has sliceable property.
Medical dressing for skin wound protection, the reparation of promotion skin wound of the present utility model has following beneficial effect:
Medical dressing of the present utility model has following advantage: 1 aseptic, nontoxic, sensitization, not carcinogenic etc. not; 2 good moisture absorption, breathability: namely wound surface is soaked stagnant, keep again the environment of little wet, little acid; 3 good absorption, attaching property: the adsorbable tangible composition that oozes out, and certain pressure is arranged; 4 certain antibiotic properties: a large amount of wound surface needs antibiotic property, spectrum, penetrance, is difficult for producing drug resistance; 5 is certain hemostatic: oozing of blood is had certain anastalsis; 6 certain not viscosity: when changing dressings not bitterly; 7 directly urge more property: product itself has short more property, can directly promote the Healing of wound; 8 good usability: it is more convenient clinically to make, and is applicable to the dressing of difformity, different parts wound surface, sliceable.
Medical dressing for skin wound protection, the reparation of promotion skin wound of the present utility model not only has above-mentioned advantage; main is bonding polypeptide and hyaluronic synergism; make medical dressing material self structure design optimization; and do not need the appositional growth factor or active somatic cell, give the function of this medical dressing material in situ induced tissue Regeneration and Repair.
Description of drawings
Fig. 1 is shown as the cross-sectional view of the medical dressing complex of repairing for skin wound protection, promotion skin wound.
Fig. 2 is shown as the light transmittance collection of illustrative plates of gel film in embodiment 2.
The specific embodiment
Further set forth this utility model below in conjunction with specific embodiment, should be understood that these embodiment only are used for explanation this utility model and are not used in restriction protection domain of the present utility model.
Embodiment 1:
A kind of medical dressing complex for skin wound protection, the reparation of promotion skin wound as shown in Figure 1; comprise the biological pluralgel film dressing 2 that can directly contact skin wound that is sealed in packaging bag 1 and away from the medical polyurethane adhesive film 3 of skin wound, the both sides of described biological pluralgel film dressing 3 also are coated with PE film 4.
Described biological pluralgel film dressing, comprise the framework material that polyanionic materials and range of polycationic substances are synthetic, be furnished with in the hole of described framework material on the molecule of polypeptide and bonding polypeptide and hyaluronic acid and/or framework material and be connected with polypeptide and bonding polypeptide and hyaluronan molecule.
Described biological pluralgel film dressing is transparent membranaceous or sponge is membranaceous.
Put adjacent with the medical polyurethane adhesive film of biological pluralgel film dressing in described packaging bag.
Further, the synthetic framework material of described polyanionic materials and range of polycationic substances is space network.
Better, described packaging bag is aluminium plastic bag.
Medical dressing complex for skin wound protection, the reparation of promotion skin wound of the present utility model; in use; biological pluralgel film dressing directly contacts skin wound as internal layer; and the medical polyurethane adhesive film is placed in the skin of biological pluralgel film dressing; directly do not contact with skin wound; biological pluralgel film dressing and the outer field medical polyurethane adhesive film of internal layer are used in conjunction with; be convenient to medical personnel and carry out cutting according to the wound surface of difformity, different parts, have sliceable property.
Embodiment 2:
(1) measure 200ml water for injection, rapid stirring, and slowly progressively add 3g carboxymethyl chitosan (polyanionic materials), stirring at normal temperature 1h toward the centre; Treat that the carboxymethyl chitosan dissolve complete adds glycerol 2ml, continued stirring and dissolving 1 hour, obtain the carboxymethyl chitosan sugar juice;
(2) take 7g chitosan (range of polycationic substances), add 150ml water for injection, add 2800ul lactic acid after stirring, then rinse with 30ml water for injection the chitosan that pastes wall of cup, stirring at normal temperature 4h obtains chitosan solution;
(3) take 3g polypeptide and bonding polypeptide, be dissolved in 20ml water for injection, again with the polypeptide on 20ml water for injection flushing walls of beaker, obtain polypeptide and bonding polypeptide solution after stirring at normal temperature is even;
(4) take the 0.01g hyaluronic acid and be dissolved in 30ml water for injection, room temperature rapid stirring 1h obtains hyaluronic acid solution;
(5) respectively the solution of above-mentioned configuration is placed in pressure cooker high temperature sterilize 30 minutes; Wherein the temperature of high temperature is 121 degrees centigrade;
(6) carboxymethyl chitosan sugar juice, polypeptide and the bonding polypeptide solution mix and blend 1h after sterilizing, then add the hyaluronic acid solution stirring at normal temperature 1h after sterilization to form mixed liquor A; Draw mixed liquor A with dropper, splash in molten 0 liquid of chitosan of high-speed stirred, commencing speed is slow, after can accelerate gradually the speed that adds of mixed liquor A, stirring at normal temperature 15h obtains mixed solution B, and the adjusting pH value is 5.8-6.4, the mixed solution B for preparing is poured in stainless steel disc, and curtain coating is even, puts under 50 ℃, baking oven and is dried into gel film; Gel film crosslinked: the gel film of drying is put into water-soluble carbodiimide and N-hydroxy-succinamide aqueous solution (concentration of water-soluble carbodiimide and N-hydroxy-succinamide is 3.5mg/ml), stirring reaction 25 hours, rinse to soak with distilled water and remove uncrosslinked composition, oven dry obtains hyaline membrane.
(7) hyaline membrane is cut to suitable size, both sides cover with the PE film, and and the abbreviation of the PU(polyurethanes of dimension) pad pasting puts into aluminium plastic bag together, aluminium plastic bag sealed.
(8) adopting cobalt-60 irradiation sterilization to process gets final product.
The hyaline membrane of above-mentioned acquisition is tested, and obtains following assay:
(1) liquid-absorbent (reference standard YY/T 0471.1-2004 contact Wound dressing test method)
The gel film of known quality (W1) 5cmx5cm is placed in the experimental liquid (experimental liquid contains the villaumite of 142mMol sodium ion and 2.5mMol calcium ion) 30 minutes of 37 ℃, then clamps gel film one end with tweezers, the 30s that dangles, weighing (W2).
Result is calculated: absorbtivity=(quality-known quality after imbibition) * 100/25(g/100cm
2); Or: the * 100/W1(% of absorbance=(W2-W1)).
From acquired results as can be known this gel film absorbtivity be every 100cm
2Absorb liquid greater than 17 grams, absorbance can satisfy the requirement that general wound surface absorbs oozing of blood and sepage between 500%-800%.
(2) comfortableness (reference standard YY/T 0471.4-2004 contact Wound dressing test method)
From cutting out the sample of wide 25.0 ± 0.5mm for the examination material, after taking off sample, allow its lax 300s, do the parallel labelling that two spacings are 100 ± 10mm on sample, and spacing is equated.Measure the distance L 1 between two labellings, be accurate to 0.5mm.Be sandwiched in two chucks of extensiometer beyond the sample labelling, and make sample elongation 20% with the draw speed of 300 ± 10mm/min, record this maximum load ML, be accurate to 0.1N, keep 60s at this stretch position, take off sample from chuck, lax 300 ± 15s, the distance L 2 on the sample of resurveying between two labellings.
Result is calculated:
Extensibility computing formula: E=ML/2.5;
Permanent deformation computing formula: PS (%)=[(L2-L1)/L1] * 100.
From acquired results as can be known: this gel film hot strength 4.5 ~ 5.0 newton/centimetre, elongation at break is 60% ~ 80%, meets the comfortableness requirement.
(3) water vapour permeability test (reference standard YY/T 0471.2-2004 ventilated membrane dressing moisture-vapor transmission):
Add suitable quantity of water in rustless steel circle cup (sectional area is 10cm2), dressing is cut into the clamping plate size the same, cover at rim of a cup, put clamping plate, clamp dressing, the water in cup can only be contacted by dressing, weighing recording quality W1 with external environment condition; Put it in the baking oven of 37 ℃, put into simultaneously the silica gel of 1kg in baking oven to keep the drying of environment; Take out from baking oven after 24 hours, weighing recording quality W2, do 5 parallel, average;
Result is calculated: water vapour permeability (MVTR): X=(W1-W2) * 1000(g/ (m
2* 24h))
From acquired results as can be known: this gel film every square metre of moisture-vapor transmission every day is about 2000 grams.
(4) light transmittance test:
Film to be measured is cut into 5cm * 2cm size, is close to a side of cuvette, be placed in the sample cell of ultraviolet-uisible spectrophotometer, measure a light transmittance every 10nm in 380 ~ 780nm wave-length coverage.Do blank with empty ware.
The light transmittance of this gel film as shown in Figure 2, from acquired results as can be known: when wavelength during less than 410nm, light transmittance is less than 50%, when wavelength during less than 550nm, light transmittance is less than 80%, when 550nm was above, light transmittance can satisfy organizing the observation of blood oozing from the wound surface, inflammation and healing state between 80%-85% fully when wavelength.
(5) mouse fibroblast cell NIH-3T3 and gel film material external united cultivation:
Mouse embryo fibroblasts is with 5 * 10
6/ ml cell density is seeded in the gel film material surface, at 37 ℃, 5%CO
2Incubator in cultivated 1-5 days, change every other day liquid, utilize the adhesion growing state of phase contrast microscope observation of cell.
Cell is after plantation 1d on the gel film material, and most cells present adherent extended configuration, and cell is the shuttle type, illustrates that the gel film material surface can promote cell to the adhesion of material itself, makes cell can adhere to as early as possible material surface, difficult drop-off; After plantation 5d, cell has covered with the covering gel film surface substantially, and it is adherent that cell is the rib type at the gel film material surface.
Embodiment 3:
(1) measure 200ml water for injection, rapid stirring, and slowly progressively add 3g polyglutamic acid (polyanionic materials), stirring at normal temperature 1h toward the centre; Treat that the polyglutamic acid dissolve complete adds glycerol 2ml, continued stirring and dissolving 1 hour, obtain polyglutamic acid solution;
(2) take 7g chitosan (range of polycationic substances), add 150ml water for injection, add 2800ul lactic acid after stirring, rinse with 30ml water for injection the chitosan that pastes wall of cup again, the pH value of controlling the aqueous solution of the lactic acid that adds is 1.0-4.0, stirring at normal temperature 4h obtains chitosan solution;
(3) take 1g polypeptide and bonding polypeptide, be dissolved in 20ml water for injection, again with the polypeptide on 20ml water for injection flushing walls of beaker, obtain polypeptide and bonding polypeptide solution after stirring at normal temperature is even;
(4) take the 0.05g hyaluronic acid and be dissolved in 25ml water for injection, room temperature rapid stirring 1h obtains hyaluronic acid solution;
(5) respectively the solution of above-mentioned configuration is placed in pressure cooker high temperature sterilize 60 minutes; Wherein the temperature of high temperature is 116 degrees centigrade;
(6) polyglutamic acid solution, polypeptide and the bonding polypeptide solution mix and blend 1h after sterilizing, then add the hyaluronic acid solution stirring at normal temperature 1h after sterilization to form mixed liquor A; Draw mixed liquor A with dropper, splash in the chitosan solution of high-speed stirred, commencing speed is slow, after can accelerate gradually the speed that adds of mixed liquor A, stirring at normal temperature 15h obtains mixed solution B, and the adjusting pH value is 5.8-6.4, the mixed solution B for preparing is poured in stainless steel disc, and curtain coating is even, puts under 50 ℃, baking oven and is dried into gel film; Gel film crosslinked: the gel film of drying is put into water-soluble carbodiimide and N-hydroxy thiosuccinimide aqueous solution (concentration of water-soluble carbodiimide and N-hydroxy thiosuccinimide is 1mg/ml), stirring reaction 25 hours, rinse to soak with distilled water and remove uncrosslinked composition, oven dry obtains hyaline membrane.
(7) hyaline membrane is cut to suitable size, both sides cover with the PE film, and and the abbreviation of the PU(polyurethanes of dimension) pad pasting puts into aluminium plastic bag together, aluminium plastic bag sealed.
(8) adopting cobalt-60 irradiation sterilization to process gets final product.
The hyaline membrane of above-mentioned acquisition is tested, and obtains following assay:
(1) liquid-absorbent (reference standard YY/T 0471.1-2004 contact Wound dressing test method)
Method according to embodiment 2 is carried out the Liquid Absorption property testing.From acquired results as can be known this gel film absorbtivity be every 100cm
2Absorb liquid greater than 17 grams, absorbance can satisfy the requirement that general wound surface absorbs oozing of blood and sepage between 500%-800%.
(2) comfortableness (reference standard YY/T 0471.4-2004 contact Wound dressing test method)
Carry out the comfortableness test according to the method for embodiment 2.From acquired results as can be known: this gel film hot strength 4.5 ~ 5.0 newton/centimetre, elongation at break is 60% ~ 80%, meets the comfortableness requirement.
(3) water vapour permeability test (reference standard YY/T 0471.2-2004 ventilated membrane dressing moisture-vapor transmission):
Carry out the water vapour permeability test according to the method for embodiment 2.From acquired results as can be known: this gel film every square metre of moisture-vapor transmission every day is about 2000 grams.
(4) light transmittance test:
Carry out the light transmittance test according to the method for embodiment 2.From the acquired results light transmittance of this gel film as can be known: when wavelength during less than 410nm, light transmittance is less than 50%, when wavelength during less than 550nm, light transmittance is less than 80%, when wavelength when 550nm is above, light transmittance can satisfy organizing the observation of blood oozing from the wound surface, inflammation and healing state between 80%-85% fully.
(5) mouse fibroblast cell NIH-3T3 and gel film material external united cultivation:
Carry out the external associating culture experiment of mouse fibroblast cell NIH-3T3 and gel film material according to the method for embodiment 2.
From experimental result as can be known: cell is after gel film material plantation 1d, most cells present adherent extended configuration, and cell is the shuttle type, illustrates that the gel film material surface can promote cell to the adhesion of material itself, make cell can adhere to as early as possible material surface, difficult drop-off; After plantation 5d, cell has covered with the covering gel film surface substantially, and it is adherent that cell is the rib type at the gel film material surface.
Embodiment 4:
(1) measure 200ml water for injection, rapid stirring, and slowly progressively add 1.5g carboxymethyl chitosan and 1.5g polyglutamic acid (polyanionic materials), stirring at normal temperature 1h toward the centre; Treat that the hyaluronic acid dissolve complete adds glycerol 2ml, continued stirring and dissolving 1 hour, obtain the solution of carboxymethyl chitosan and polyglutamic acid;
(2) take 7g chitosan (range of polycationic substances), add 150ml water for injection, add 2800ul lactic acid after stirring, then rinse with 30ml water for injection the chitosan that pastes wall of cup, stirring at normal temperature 4h, the solution of acquisition chitosan;
(3) take 2g polypeptide and bonding polypeptide, be dissolved in 20ml water for injection, again with the polypeptide on 20ml water for injection flushing walls of beaker, obtain the solution of polypeptide and bonding polypeptide after stirring at normal temperature is even;
(4) take the 0.02g hyaluronic acid and be dissolved in 30ml water for injection, room temperature rapid stirring 1h obtains hyaluronic solution;
(5) respectively the solution of above-mentioned configuration is placed in pressure cooker high temperature sterilize 30 minutes; Wherein the temperature of high temperature is 125 degrees centigrade;
(6) carboxymethyl chitosan and polyglutamic acid solution, polypeptide and the bonding polypeptide solution mix and blend 1h after sterilizing, then add the hyaluronic acid solution stirring at normal temperature 1h after sterilization to form mixed liquor A; Draw mixed liquor A with dropper, splash in the chitosan solution of high-speed stirred, commencing speed is slow, after can accelerate gradually the speed that adds of mixed liquor A, stirring at normal temperature 15h obtains mixed solution B, and the adjusting pH value is 5.8-6.4, the mixed solution B for preparing is poured in stainless steel disc, and curtain coating is even, puts under 50 ℃, baking oven and is dried into gel film; Gel film crosslinked: the gel film of drying is put into water-soluble carbodiimide and N-hydroxy-succinamide aqueous solution (concentration of water-soluble carbodiimide and N-hydroxy-succinamide is 2.5mg/ml), stirring reaction 25 hours, rinse to soak with distilled water and remove uncrosslinked composition, oven dry obtains hyaline membrane.
(7) hyaline membrane is cut to suitable size, both sides cover with the PE film, and and the abbreviation of PU(polyurethanes) pad pasting puts into aluminium plastic bag together, aluminium plastic bag sealed.
(8) adopting cobalt-60 irradiation sterilization to process gets final product.
The hyaline membrane of above-mentioned acquisition is tested, and obtains following assay:
(1) liquid-absorbent (reference standard YY/T 0471.1-2004 contact Wound dressing test method)
Method according to embodiment 2 is carried out the Liquid Absorption property testing.From acquired results as can be known this gel film absorbtivity be every 100cm
2Absorb liquid greater than 17 grams, absorbance can satisfy the requirement that general wound surface absorbs oozing of blood and sepage between 500%-800%.
(2) comfortableness (reference standard YY/T 0471.4-2004 contact Wound dressing test method)
Carry out the comfortableness test according to the method for embodiment 2.From acquired results as can be known: this gel film hot strength 4.5 ~ 5.0 newton/centimetre, elongation at break is 60% ~ 80%, meets the comfortableness requirement.
(3) water vapour permeability test (reference standard YY/T 0471.2-2004 ventilated membrane dressing moisture-vapor transmission):
Carry out the water vapour permeability test according to the method for embodiment 2.From acquired results as can be known: this gel film every square metre of moisture-vapor transmission every day is about 2000 grams.
(4) light transmittance test:
Carry out the light transmittance test according to the method for embodiment 2.From the acquired results light transmittance of this gel film as can be known: when wavelength during less than 410nm, light transmittance is less than 50%, when wavelength during less than 550nm, light transmittance is less than 80%, when wavelength when 550nm is above, light transmittance can satisfy organizing the observation of blood oozing from the wound surface, inflammation and healing state between 80%-85% fully.
(5) mouse fibroblast cell NIH-3T3 and gel film material external united cultivation:
Carry out the external associating culture experiment of mouse fibroblast cell NIH-3T3 and gel film material according to the method for embodiment 2.
From experimental result as can be known: cell is after gel film material plantation 1d, most cells present adherent extended configuration, and cell is the shuttle type, illustrates that the gel film material surface can promote cell to the adhesion of material itself, make cell can adhere to as early as possible material surface, difficult drop-off; After plantation 5d, cell has covered with the covering gel film surface substantially, and it is adherent that cell is the rib type at the gel film material surface.
Embodiment 5:
Except the range of polycationic substances in step (2) is polylysine, the acid of the solubilized range of polycationic substances that adds is outside acetic acid and except the gel film of drying is further not crosslinked, all the other method steps are identical with embodiment 2, obtain the biological pluralgel film dressing of the present embodiment, are hyaline membrane.
The hyaline membrane of above-mentioned acquisition is tested, and obtains following assay:
(1) liquid-absorbent (reference standard YY/T 0471.1-2004 contact Wound dressing test method)
Method according to embodiment 2 is carried out the Liquid Absorption property testing.From acquired results as can be known this gel film absorbtivity be every 100cm
2Absorb liquid greater than 17 grams, absorbance can satisfy the requirement that general wound surface absorbs oozing of blood and sepage between 500%-800%.
(2) comfortableness (reference standard YY/T 0471.4-2004 contact Wound dressing test method)
Carry out the comfortableness test according to the method for embodiment 2.From acquired results as can be known: this gel film hot strength 4.5 ~ 5.0 newton/centimetre, elongation at break is 60% ~ 80%, meets the comfortableness requirement.
(3) water vapour permeability test (reference standard YY/T 0471.2-2004 ventilated membrane dressing moisture-vapor transmission):
Carry out the water vapour permeability test according to the method for embodiment 2.From acquired results as can be known: this gel film every square metre of moisture-vapor transmission every day is about 2000 grams.
(4) light transmittance test:
Carry out the light transmittance test according to the method for embodiment 2.From the acquired results light transmittance of this gel film as can be known: when wavelength during less than 410nm, light transmittance is less than 50%, when wavelength during less than 550nm, light transmittance is less than 80%, when wavelength when 550nm is above, light transmittance can satisfy organizing the observation of blood oozing from the wound surface, inflammation and healing state between 80%-85% fully.
(5) mouse fibroblast cell NIH-3T3 and gel film material external united cultivation:
Carry out the external associating culture experiment of mouse fibroblast cell NIH-3T3 and gel film material according to the method for embodiment 2.
From experimental result as can be known: cell is after gel film material plantation 1d, most cells present adherent extended configuration, and cell is the shuttle type, illustrates that the gel film material surface can promote cell to the adhesion of material itself, make cell can adhere to as early as possible material surface, difficult drop-off; After plantation 5d, cell has covered with the covering gel film surface substantially, and it is adherent that cell is the rib type at the gel film material surface.
Embodiment 6:
Polyanionic materials in step (1) is alginic acid or alginate, and all the other method steps are identical with embodiment 2, obtains the biological pluralgel film dressing of the present embodiment, is hyaline membrane.
The hyaline membrane of above-mentioned acquisition is tested, and obtains following assay:
(1) liquid-absorbent (reference standard YY/T 0471.1-2004 contact Wound dressing test method)
Method according to embodiment 2 is carried out the Liquid Absorption property testing.From acquired results as can be known this gel film absorbtivity be every 100cm
2Absorb liquid greater than 17 grams, absorbance can satisfy the requirement that general wound surface absorbs oozing of blood and sepage between 500%-800%.
(2) comfortableness (reference standard YY/T 0471.4-2004 contact Wound dressing test method)
Carry out the comfortableness test according to the method for embodiment 2.From acquired results as can be known: this gel film hot strength 4.5 ~ 5.0 newton/centimetre, elongation at break is 60% ~ 80%, meets the comfortableness requirement.
(3) water vapour permeability test (reference standard YY/T 0471.2-2004 ventilated membrane dressing moisture-vapor transmission):
Carry out the water vapour permeability test according to the method for embodiment 2.From acquired results as can be known: this gel film every square metre of moisture-vapor transmission every day is about 2000 grams.
(4) light transmittance test:
Carry out the light transmittance test according to the method for embodiment 2.From the acquired results light transmittance of this gel film as can be known: when wavelength during less than 410nm, light transmittance is less than 50%, when wavelength during less than 550nm, light transmittance is less than 80%, when wavelength when 550nm is above, light transmittance can satisfy organizing the observation of blood oozing from the wound surface, inflammation and healing state between 80%-85% fully.
(5) mouse fibroblast cell NIH-3T3 and gel film material external united cultivation:
Carry out the external associating culture experiment of mouse fibroblast cell NIH-3T3 and gel film material according to the method for embodiment 2.
From experimental result as can be known: cell is after gel film material plantation 1d, most cells present adherent extended configuration, and cell is the shuttle type, illustrates that the gel film material surface can promote cell to the adhesion of material itself, make cell can adhere to as early as possible material surface, difficult drop-off; After plantation 5d, cell has covered with the covering gel film surface substantially, and it is adherent that cell is the rib type at the gel film material surface.
Embodiment 7:
Polyanionic materials in step (1) is collagen, and all the other method steps are identical with embodiment 2, obtains the biological pluralgel film dressing of the present embodiment, is hyaline membrane.
The hyaline membrane of above-mentioned acquisition is tested, and obtains following assay:
(1) liquid-absorbent (reference standard YY/T 0471.1-2004 contact Wound dressing test method)
Method according to embodiment 2 is carried out the Liquid Absorption property testing.From acquired results as can be known this gel film absorbtivity be every 100cm
2Absorb liquid greater than 17 grams, absorbance can satisfy the requirement that general wound surface absorbs oozing of blood and sepage between 500%-800%.
(2) comfortableness (reference standard YY/T 0471.4-2004 contact Wound dressing test method)
Carry out the comfortableness test according to the method for embodiment 2.From acquired results as can be known: this gel film hot strength 4.5 ~ 5.0 newton/centimetre, elongation at break is 60% ~ 80%, meets the comfortableness requirement.
(3) water vapour permeability test (reference standard YY/T 0471.2-2004 ventilated membrane dressing moisture-vapor transmission):
Carry out the water vapour permeability test according to the method for embodiment 2.From acquired results as can be known: this gel film every square metre of moisture-vapor transmission every day is about 2000 grams.
(4) light transmittance test:
Carry out the light transmittance test according to the method for embodiment 2.From the acquired results light transmittance of this gel film as can be known: when wavelength during less than 410nm, light transmittance is less than 50%, when wavelength during less than 550nm, light transmittance is less than 80%, when wavelength when 550nm is above, light transmittance can satisfy organizing the observation of blood oozing from the wound surface, inflammation and healing state between 80%-85% fully.
(5) mouse fibroblast cell NIH-3T3 and gel film material external united cultivation:
Carry out the external associating culture experiment of mouse fibroblast cell NIH-3T3 and gel film material according to the method for embodiment 2.
From experimental result as can be known: cell is after gel film material plantation 1d, most cells present adherent extended configuration, and cell is the shuttle type, illustrates that the gel film material surface can promote cell to the adhesion of material itself, make cell can adhere to as early as possible material surface, difficult drop-off; After plantation 5d, cell has covered with the covering gel film surface substantially, and it is adherent that cell is the rib type at the gel film material surface.
Embodiment 8:
Polyanionic materials in step (1) is that (its viscosity-average molecular weight is 1,500,000 to hyaluronic acid, and all the other method steps are identical with embodiment 2, obtains the biological pluralgel film dressing of the present embodiment, is hyaline membrane.
The hyaline membrane of above-mentioned acquisition is tested, and obtains following assay:
(1) liquid-absorbent (reference standard YY/T 0471.1-2004 contact Wound dressing test method)
Method according to embodiment 2 is carried out the Liquid Absorption property testing.From acquired results as can be known this gel film absorbtivity be every 100cm
2Absorb liquid greater than 17 grams, absorbance can satisfy the requirement that general wound surface absorbs oozing of blood and sepage between 500%-800%.
(2) comfortableness (reference standard YY/T 0471.4-2004 contact Wound dressing test method)
Carry out the comfortableness test according to the method for embodiment 2.From acquired results as can be known: this gel film hot strength 4.5 ~ 5.0 newton/centimetre, elongation at break is 60% ~ 80%, meets the comfortableness requirement.
(3) water vapour permeability test (reference standard YY/T 0471.2-2004 ventilated membrane dressing moisture-vapor transmission):
Carry out the water vapour permeability test according to the method for embodiment 2.From acquired results as can be known: this gel film every square metre of moisture-vapor transmission every day is about 2000 grams.
(4) light transmittance test:
Carry out the light transmittance test according to the method for embodiment 2.From the acquired results light transmittance of this gel film as can be known: when wavelength during less than 410nm, light transmittance is less than 50%, when wavelength during less than 550nm, light transmittance is less than 80%, when wavelength when 550nm is above, light transmittance can satisfy organizing the observation of blood oozing from the wound surface, inflammation and healing state between 80%-85% fully.
(5) mouse fibroblast cell NIH-3T3 and gel film material external united cultivation:
Carry out the external associating culture experiment of mouse fibroblast cell NIH-3T3 and gel film material according to the method for embodiment 2.
From experimental result as can be known: cell is after gel film material plantation 1d, most cells present adherent extended configuration, and cell is the shuttle type, illustrates that the gel film material surface can promote cell to the adhesion of material itself, make cell can adhere to as early as possible material surface, difficult drop-off; After plantation 5d, cell has covered with the covering gel film surface substantially, and it is adherent that cell is the rib type at the gel film material surface.
Embodiment 9:
Polyanionic materials in step (1) is carboxymethyl cellulose, and all the other method steps are identical with embodiment 2, obtains the biological pluralgel film dressing of the present embodiment, is hyaline membrane.
The hyaline membrane of above-mentioned acquisition is tested, and obtains following assay:
(1) liquid-absorbent (reference standard YY/T 0471.1-2004 contact Wound dressing test method)
Method according to embodiment 2 is carried out the Liquid Absorption property testing.From acquired results as can be known this gel film absorbtivity be every 100cm
2Absorb liquid greater than 17 grams, absorbance can satisfy the requirement that general wound surface absorbs oozing of blood and sepage between 500%-800%.
(2) comfortableness (reference standard YY/T 0471.4-2004 contact Wound dressing test method)
Carry out the comfortableness test according to the method for embodiment 2.From acquired results as can be known: this gel film hot strength 4.5 ~ 5.0 newton/centimetre, elongation at break is 60% ~ 80%, meets the comfortableness requirement.
(3) water vapour permeability test (reference standard YY/T 0471.2-2004 ventilated membrane dressing moisture-vapor transmission):
Carry out the water vapour permeability test according to the method for embodiment 2.From acquired results as can be known: this gel film every square metre of moisture-vapor transmission every day is about 2000 grams.
(4) light transmittance test:
Carry out the light transmittance test according to the method for embodiment 2.From the acquired results light transmittance of this gel film as can be known: when wavelength during less than 410nm, light transmittance is less than 50%, when wavelength during less than 550nm, light transmittance is less than 80%, when wavelength when 550nm is above, light transmittance can satisfy organizing the observation of blood oozing from the wound surface, inflammation and healing state between 80%-85% fully.
(5) mouse fibroblast cell NIH-3T3 and gel film material external united cultivation:
Carry out the external associating culture experiment of mouse fibroblast cell NIH-3T3 and gel film material according to the method for embodiment 2.
From experimental result as can be known: cell is after gel film material plantation 1d, most cells present adherent extended configuration, and cell is the shuttle type, illustrates that the gel film material surface can promote cell to the adhesion of material itself, make cell can adhere to as early as possible material surface, difficult drop-off; After plantation 5d, cell has covered with the covering gel film surface substantially, and it is adherent that cell is the rib type at the gel film material surface.
Embodiment 10:
Polyanionic materials in step (1) is carboxymethyl starch, and all the other method steps are identical with embodiment 2, obtains the biological pluralgel film dressing of the present embodiment, is hyaline membrane.
The hyaline membrane of above-mentioned acquisition is tested, and obtains following assay:
(1) liquid-absorbent (reference standard YY/T 0471.1-2004 contact Wound dressing test method)
Method according to embodiment 2 is carried out the Liquid Absorption property testing.From acquired results as can be known this gel film absorbtivity be every 100cm
2Absorb liquid greater than 17 grams, absorbance can satisfy the requirement that general wound surface absorbs oozing of blood and sepage between 500%-800%.
(2) comfortableness (reference standard YY/T 0471.4-2004 contact Wound dressing test method)
Carry out the comfortableness test according to the method for embodiment 2.From acquired results as can be known: this gel film hot strength 4.5 ~ 5.0 newton/centimetre, elongation at break is 60% ~ 80%, meets the comfortableness requirement.
(3) water vapour permeability test (reference standard YY/T 0471.2-2004 ventilated membrane dressing moisture-vapor transmission):
Carry out the water vapour permeability test according to the method for embodiment 2.From acquired results as can be known: this gel film every square metre of moisture-vapor transmission every day is about 2000 grams.
(4) light transmittance test:
Carry out the light transmittance test according to the method for embodiment 2.From the acquired results light transmittance of this gel film as can be known: when wavelength during less than 410nm, light transmittance is less than 50%, when wavelength during less than 550nm, light transmittance is less than 80%, when wavelength when 550nm is above, light transmittance can satisfy organizing the observation of blood oozing from the wound surface, inflammation and healing state between 80%-85% fully.
(5) mouse fibroblast cell NIH-3T3 and gel film material external united cultivation:
Carry out the external associating culture experiment of mouse fibroblast cell NIH-3T3 and gel film material according to the method for embodiment 2.
From experimental result as can be known: cell is after gel film material plantation 1d, most cells present adherent extended configuration, and cell is the shuttle type, illustrates that the gel film material surface can promote cell to the adhesion of material itself, make cell can adhere to as early as possible material surface, difficult drop-off; After plantation 5d, cell has covered with the covering gel film surface substantially, and it is adherent that cell is the rib type at the gel film material surface.
Embodiment 11:
Polyanionic materials in step (1) is polyglutamic acid, and all the other method steps are identical with embodiment 1, obtains the biological pluralgel film dressing of the present embodiment, is hyaline membrane.
The hyaline membrane of above-mentioned acquisition is tested, and obtains following assay:
(1) liquid-absorbent (reference standard YY/T 0471.1-2004 contact Wound dressing test method)
Method according to embodiment 2 is carried out the Liquid Absorption property testing.From acquired results as can be known this gel film absorbtivity be every 100cm
2Absorb liquid greater than 17 grams, absorbance can satisfy the requirement that general wound surface absorbs oozing of blood and sepage between 500%-800%.
(2) comfortableness (reference standard YY/T 0471.4-2004 contact Wound dressing test method)
Carry out the comfortableness test according to the method for embodiment 2.From acquired results as can be known: this gel film hot strength 4.5 ~ 5.0 newton/centimetre, elongation at break is 60% ~ 80%, meets the comfortableness requirement.
(3) water vapour permeability test (reference standard YY/T 0471.2-2004 ventilated membrane dressing moisture-vapor transmission):
Carry out the water vapour permeability test according to the method for embodiment 2.From acquired results as can be known: this gel film every square metre of moisture-vapor transmission every day is about 2000 grams.
(4) light transmittance test:
Carry out the light transmittance test according to the method for embodiment 2.From the acquired results light transmittance of this gel film as can be known: when wavelength during less than 410nm, light transmittance is less than 50%, when wavelength during less than 550nm, light transmittance is less than 80%, when wavelength when 550nm is above, light transmittance can satisfy organizing the observation of blood oozing from the wound surface, inflammation and healing state between 80%-85% fully.
(5) mouse fibroblast cell NIH-3T3 and gel film material external united cultivation:
Carry out the external associating culture experiment of mouse fibroblast cell NIH-3T3 and gel film material according to the method for embodiment 2.
From experimental result as can be known: cell is after gel film material plantation 1d, most cells present adherent extended configuration, and cell is the shuttle type, illustrates that the gel film material surface can promote cell to the adhesion of material itself, make cell can adhere to as early as possible material surface, difficult drop-off; After plantation 5d, cell has covered with the covering gel film surface substantially, and it is adherent that cell is the rib type at the gel film material surface.
Embodiment 12:
(1) measure 200ml water for injection, rapid stirring, and slowly progressively add 2g carboxymethyl chitosan (polyanionic materials), stirring at normal temperature 1h toward the centre; Treat that the carboxymethyl chitosan dissolve complete adds glycerol 4ml, continued stirring and dissolving 1 hour, obtain the carboxymethyl chitosan sugar juice;
(2) take 8g chitosan (range of polycationic substances), add 150ml water for injection, add 2800ul lactic acid after stirring, then rinse with 30ml water for injection the chitosan that pastes wall of cup, stirring at normal temperature 4h obtains chitosan solution;
(3) take 5g polypeptide and bonding polypeptide, be dissolved in 40ml water for injection, again with the polypeptide on 20ml water for injection flushing walls of beaker, obtain polypeptide and bonding polypeptide solution after stirring at normal temperature is even;
(4) take the 0.006g hyaluronic acid and be dissolved in 30ml water for injection, room temperature rapid stirring 1h obtains hyaluronic acid solution;
(5) respectively the solution of above-mentioned configuration is placed in pressure cooker high temperature sterilize 30 minutes; Wherein the temperature of high temperature is 125 ° of C;
(6) carboxymethyl chitosan sugar juice, polypeptide and the bonding polypeptide solution mix and blend 1h after sterilizing, then add after sterilization thoroughly
Phaneroplasm acid solution stirring at normal temperature 1h forms mixed liquor A; Draw mixed liquor A with dropper, splash in the chitosan solution of high-speed stirred, commencing speed is slow, after can accelerate gradually the speed that adds of mixed liquor A, stirring at normal temperature 15h obtains mixed solution B, and the adjusting pH value is 5.8-6.4, the mixed solution B for preparing is poured in stainless steel disc, and curtain coating is even, puts under 50 ℃, baking oven and is dried into gel film; Gel film crosslinked: the gel film of drying is put into water-soluble carbodiimide and N-hydroxy-succinamide aqueous solution (concentration of water-soluble carbodiimide and N-hydroxy-succinamide is 3.5mg/ml), stirring reaction 15 hours, rinse to soak with distilled water and remove uncrosslinked composition, oven dry obtains hyaline membrane.
(7) hyaline membrane is cut to suitable size, both sides cover with the PE film, and and the abbreviation of the PU(polyurethanes of dimension) pad pasting puts into aluminium plastic bag together, aluminium plastic bag sealed.
(8) adopting cobalt-60 irradiation sterilization to process gets final product.
The hyaline membrane of above-mentioned acquisition is tested, and obtains following assay:
(1) liquid-absorbent (reference standard YY/T 0471.1-2004 contact Wound dressing test method)
Method according to embodiment 2 is carried out the Liquid Absorption property testing.From acquired results as can be known this gel film absorbtivity be every 100cm
2Absorb liquid greater than 17 grams, absorbance can satisfy the requirement that general wound surface absorbs oozing of blood and sepage between 500%-800%.
(2) comfortableness (reference standard YY/T 0471.4-2004 contact Wound dressing test method)
Carry out the comfortableness test according to the method for embodiment 2.From acquired results as can be known: this gel film hot strength 4.5 ~ 5.0 newton/centimetre, elongation at break is 60% ~ 80%, meets the comfortableness requirement.
(3) water vapour permeability test (reference standard YY/T 0471.2-2004 ventilated membrane dressing moisture-vapor transmission):
Carry out the water vapour permeability test according to the method for embodiment 2.From acquired results as can be known: this gel film every square metre of moisture-vapor transmission every day is about 2000 grams.
(4) light transmittance test:
Carry out the light transmittance test according to the method for embodiment 2.From the acquired results light transmittance of this gel film as can be known: when wavelength during less than 410nm, light transmittance is less than 50%, when wavelength during less than 550nm, light transmittance is less than 80%, when wavelength when 550nm is above, light transmittance can satisfy organizing the observation of blood oozing from the wound surface, inflammation and healing state between 80%-85% fully.
(5) mouse fibroblast cell NIH-3T3 and gel film material external united cultivation:
Carry out the external associating culture experiment of mouse fibroblast cell NIH-3T3 and gel film material according to the method for embodiment 2.
From experimental result as can be known: cell is after gel film material plantation 1d, most cells present adherent extended configuration, and cell is the shuttle type, illustrates that the gel film material surface can promote cell to the adhesion of material itself, make cell can adhere to as early as possible material surface, difficult drop-off; After plantation 5d, cell has covered with the covering gel film surface substantially, and it is adherent that cell is the rib type at the gel film material surface.
Embodiment 13:
(1) measure 200ml water for injection, rapid stirring, and slowly progressively add 4g carboxymethyl chitosan (polyanionic materials), stirring at normal temperature 1h toward the centre; Treat that the carboxymethyl chitosan dissolve complete adds glycerol 3ml, continued stirring and dissolving 1 hour, obtain the carboxymethyl chitosan sugar juice;
(2) take 6g chitosan (range of polycationic substances), add 80ml water for injection, add 2500ul lactic acid after stirring, then rinse with 20ml water for injection the chitosan that pastes wall of cup, stirring at normal temperature 4h obtains chitosan solution;
(3) take 5g polypeptide and bonding polypeptide, be dissolved in 30ml water for injection, again with the polypeptide on 20ml water for injection flushing walls of beaker, obtain polypeptide and bonding polypeptide solution after stirring at normal temperature is even;
(4) take the 0.01g hyaluronic acid and be dissolved in 30ml water for injection, room temperature rapid stirring 1h obtains hyaluronic acid solution;
(5) respectively the solution of above-mentioned configuration is placed in pressure cooker high temperature sterilize 30 minutes; Wherein the temperature of high temperature is 130 ° of C;
(6) carboxymethyl chitosan sugar juice, polypeptide and the bonding polypeptide solution mix and blend 1h after sterilizing, then add the hyaluronic acid solution stirring at normal temperature 1h after sterilization to form mixed liquor A; Draw mixed liquor A with dropper, splash in the chitosan solution of high-speed stirred, commencing speed is slow, after can accelerate gradually the speed that adds of mixed liquor A, stirring at normal temperature 15h obtains mixed solution B, and the adjusting pH value is 5.8-6.4, the mixed solution B for preparing is poured in stainless steel disc, and curtain coating is even, and natural drying becomes gel film; Gel film crosslinked: the gel film of drying is put into water-soluble carbodiimide and N-hydroxy-succinamide aqueous solution (concentration of water-soluble carbodiimide and N-hydroxy-succinamide is 3.5mg/ml), stirring reaction 20 hours, rinse to soak with distilled water and remove uncrosslinked composition, lyophilization obtains sponge membrane.
(7) sponge membrane is cut to suitable size, both sides cover with the PE film, and and the abbreviation of the PU(polyurethanes of dimension) pad pasting puts into aluminium plastic bag together, aluminium plastic bag sealed.
(8) adopting cobalt-60 irradiation sterilization to process gets final product.
The sponge membrane of above-mentioned acquisition is tested, and obtains following assay:
(1) liquid-absorbent (reference standard YY/T 0471.1-2004 contact Wound dressing test method)
Method according to embodiment 2 is carried out the Liquid Absorption property testing.From acquired results as can be known this gel film absorbtivity be every 100cm
2Absorb liquid greater than 17 grams, absorbance can satisfy the requirement that general wound surface absorbs oozing of blood and sepage between 500%-800%.
(2) comfortableness (reference standard YY/T 0471.4-2004 contact Wound dressing test method)
Carry out the comfortableness test according to the method for embodiment 2.From acquired results as can be known: this gel film hot strength 4.5 ~ 5.0 newton/centimetre, elongation at break is 60% ~ 80%, meets the comfortableness requirement.
(3) water vapour permeability test (reference standard YY/T 0471.2-2004 ventilated membrane dressing moisture-vapor transmission):
Carry out the water vapour permeability test according to the method for embodiment 2.From acquired results as can be known: this gel film every square metre of moisture-vapor transmission every day is about 2000 grams.
(4) light transmittance test:
Carry out the light transmittance test according to the method for embodiment 2.From the acquired results light transmittance of this gel film as can be known: when wavelength during less than 410nm, light transmittance is less than 50%, when wavelength during less than 550nm, light transmittance is less than 80%, when wavelength when 550nm is above, light transmittance can satisfy organizing the observation of blood oozing from the wound surface, inflammation and healing state between 80%-85% fully.
(5) mouse fibroblast cell NIH-3T3 and gel film material external united cultivation:
Carry out the external associating culture experiment of mouse fibroblast cell NIH-3T3 and gel film material according to the method for embodiment 2.
From experimental result as can be known: cell is after gel film material plantation 1d, most cells present adherent extended configuration, and cell is the shuttle type, illustrates that the gel film material surface can promote cell to the adhesion of material itself, make cell can adhere to as early as possible material surface, difficult drop-off; After plantation 5d, cell has covered with the covering gel film surface substantially, and it is adherent that cell is the rib type at the gel film material surface.
Above-described embodiment is illustrative principle of the present utility model and effect thereof only, but not is used for restriction this utility model.Any person skilled in the art scholar all can under spirit of the present utility model and category, modify or change above-described embodiment.Therefore, have in technical field under such as and know that usually the knowledgeable modifies or changes not breaking away from all equivalences of completing under spirit that this utility model discloses and technological thought, must be contained by claim of the present utility model.
Claims (8)
1. active dressing of skin wound biotic induce, it is characterized in that, comprise the framework material that polyanionic materials and range of polycationic substances are synthetic, be furnished with in the hole of described framework material on the molecule of polypeptide and bonding polypeptide and hyaluronic acid and/or framework material and be connected with polypeptide and bonding polypeptide and hyaluronan molecule.
2. the active dressing of skin wound biotic induce according to claim 1 is characterized in that, the active dressing of described skin wound biotic induce is transparent membranaceous or sponge is membranaceous.
3. the active dressing of skin wound biotic induce according to claim 1 is characterized in that the synthetic framework material of described polyanionic materials and range of polycationic substances is space network.
4. protect, promote for skin wound the medical dressing complex that skin wound is repaired for one kind; it is characterized in that; comprise the active dressing of the skin wound biotic induce that can directly contact skin wound that is sealed in packaging bag and away from the medical polyurethane adhesive film of skin wound, the both sides of the active dressing of described skin wound biotic induce also are coated with the PE film.
5. medical dressing complex according to claim 4, it is characterized in that, the active dressing of described skin wound biotic induce, comprise the framework material that polyanionic materials and range of polycationic substances are synthetic, be furnished with in the hole of described framework material on the molecule of polypeptide and bonding polypeptide and hyaluronic acid and/or framework material and be connected with polypeptide and bonding polypeptide and hyaluronan molecule.
6. medical dressing complex according to claim 4, is characterized in that, the active dressing of described skin wound biotic induce is transparent membranaceous or sponge is membranaceous.
7. medical dressing complex according to claim 4, is characterized in that, the synthetic framework material of described polyanionic materials and range of polycationic substances is space network.
8. according to claim 4-7 arbitrary described medical dressing complex, is characterized in that, put adjacent with the medical polyurethane adhesive film of biological pluralgel film dressing in described packaging bag.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201220205537 CN202920687U (en) | 2012-05-09 | 2012-05-09 | Biological inductive active dressing for skin wound and medical dressing compound |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201220205537 CN202920687U (en) | 2012-05-09 | 2012-05-09 | Biological inductive active dressing for skin wound and medical dressing compound |
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104784741A (en) * | 2015-04-23 | 2015-07-22 | 武汉市思泰利医疗器械发展有限公司 | Functional medical dressing containing chitosan and hydrocolloid |
| CN107502048A (en) * | 2017-09-16 | 2017-12-22 | 常州豪坦商贸有限公司 | A kind of preparation method of water-based ink |
| CN109045349A (en) * | 2018-09-27 | 2018-12-21 | 四川省原子能研究院 | A kind of hydrogel skin wound dressing and preparation method thereof that antibacterial heal-promoting closes |
| CN113174092A (en) * | 2021-05-17 | 2021-07-27 | 西安交通大学 | Polyion compound of hyaluronic acid-epsilon-polylysine hydrochloride, preparation method and application |
| CN114984304A (en) * | 2022-07-13 | 2022-09-02 | 温州市安多多医疗器械有限公司 | Antibacterial hemagglutination-stopping rubber powder and preparation method thereof |
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2012
- 2012-05-09 CN CN 201220205537 patent/CN202920687U/en not_active Expired - Lifetime
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104784741A (en) * | 2015-04-23 | 2015-07-22 | 武汉市思泰利医疗器械发展有限公司 | Functional medical dressing containing chitosan and hydrocolloid |
| CN107502048A (en) * | 2017-09-16 | 2017-12-22 | 常州豪坦商贸有限公司 | A kind of preparation method of water-based ink |
| CN109045349A (en) * | 2018-09-27 | 2018-12-21 | 四川省原子能研究院 | A kind of hydrogel skin wound dressing and preparation method thereof that antibacterial heal-promoting closes |
| CN113174092A (en) * | 2021-05-17 | 2021-07-27 | 西安交通大学 | Polyion compound of hyaluronic acid-epsilon-polylysine hydrochloride, preparation method and application |
| CN113174092B (en) * | 2021-05-17 | 2022-10-25 | 西安交通大学 | Polyion compound of hyaluronic acid-epsilon-polylysine hydrochloride, preparation method and application |
| CN114984304A (en) * | 2022-07-13 | 2022-09-02 | 温州市安多多医疗器械有限公司 | Antibacterial hemagglutination-stopping rubber powder and preparation method thereof |
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