CN116391806B - Coated tannic acid preparation for weaned piglets and preparation method and use thereof - Google Patents
Coated tannic acid preparation for weaned piglets and preparation method and use thereof Download PDFInfo
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- CN116391806B CN116391806B CN202310407918.7A CN202310407918A CN116391806B CN 116391806 B CN116391806 B CN 116391806B CN 202310407918 A CN202310407918 A CN 202310407918A CN 116391806 B CN116391806 B CN 116391806B
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- tannic acid
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- weaned pigs
- coated
- hesperidin
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- 235000015097 nutrients Nutrition 0.000 description 1
- 230000004792 oxidative damage Effects 0.000 description 1
- 230000036211 photosensitivity Effects 0.000 description 1
- 229920000642 polymer Polymers 0.000 description 1
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- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
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- A23K10/30—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
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Abstract
The invention belongs to the technical field of feed additives, and particularly relates to a coated tannic acid preparation for weaned pigs, and a preparation method and application thereof. Aiming at the problems of high diarrhea rate, low feed intake, reduced immune function and poor growth vigor of the existing weaned pigs, the invention provides a coated tannic acid preparation for weaned pigs. The coated tannic acid preparation for weaned pigs comprises, by weight, 70-80% of main materials and 20-30% of auxiliary materials, wherein the main materials are mixtures of tannic acid, chlorogenic acid and hesperidin, and the auxiliary materials are mixtures of corn starch, beta-cyclodextrin, sodium alginate, palm oil, acrylic resin and hydroxypropyl methylcellulose acetate succinate. The invention also provides a preparation method and application of the preparation. The preparation provided by the invention can improve the palatability of feed, reduce the diarrhea rate of weaned pigs, repair damaged intestinal tracts of weaned pigs, maintain the balance of intestinal microbial thalli and improve the disease resistance of weaned pigs.
Description
Technical Field
The invention belongs to the technical field of feed additives, and particularly relates to a coated tannic acid preparation for weaned pigs, and a preparation method and application thereof.
Background
In order to increase the number of litter size in the year, avoid the cross infection of sow and piglet diseases, increase economic benefit and generally implement early weaning on piglets in production. However, early weaning can cause stress to piglets, affect the morphological structure of intestinal tissues and the balance of intestinal microorganisms of the piglets, and easily cause problems of decreased feed intake, disorder of digestive system, diarrhea and the like of the piglets. How to avoid diarrhea of weaned pigs and improve intestinal health and disease resistance of the weaned pigs is a focus of continuous attention in the industry.
Along with the great reduction of zinc oxide consumption in feeds in China and the prohibition of adding growth promoting medicine feed additives, how to effectively prevent and control diarrhea of piglets through other ways becomes one of the common difficulties in the industry. Under the background of comprehensive resistance limiting and resistance forbidding, the replacement resistance product becomes an urgent need for healthy development in the field of weaned piglet cultivation.
The hydrolyzed tannin is also called tannic acid or tannic acid, has a relative molecular weight of 500-3000, and can be hydrolyzed into ellagic acid, gallic acid and glucose. The hydrolyzed tannin is used as a polyphenol compound and has stronger biological characteristics and pharmacological activity. Research at home and abroad shows that the application potential of the hydrolyzed tannin in animal nutrition is huge, and the hydrolyzed tannin from sweet chestnut wood is widely applied as a feed additive in Europe. The abuse of antibiotics in current animal farming production processes leads to serious food safety problems and the creation of superbacteria. The hydrolyzed tannin is used as a substance with pharmacological activity generated by plants, has the functions of bacteriostasis, antioxidation and free radical capture, and can be used as a feed additive for replacing antibiotics to be applied to animal feed. At present, researches show that 0.7-1.5kg/t of hydrolyzed tannin is added into daily ration of weaned pigs, so that diarrhea can be reduced, production performance can be improved, and the consumption of zinc oxide can be reduced and antibiotics can be replaced. However, because the tannic acid with high content can be combined with and deposited on the sialoadhesin, coarse wrinkles and dry feel are caused, the novel problems of strong bitter taste, poor palatability, low feed intake and the like are easily caused in the weaning period of piglets, so that the immune function of the piglets is influenced, and other related diseases are easily caused.
Aiming at the problems, the invention aims to produce a novel additive specially suitable for improving the disease resistance of weaned pigs by using tannic acid, chlorogenic acid and hesperidin in a matched manner and adopting a reasonable coating technology.
Chlorogenic acid, also known as caffeic tannic acid, is widely found in dicotyledonous plants and ferns, especially eucommia ulmoides, honeysuckle, chrysanthemum and other plants in a rich content. Few varieties of plant origin are available, and the supply of chlorogenic acid in large quantities is limited due to the limitations of the yield and content of existing plants, the price still being high. In addition, chlorogenic acid is independently used as a feed additive, so that the effect in production practice of the chlorogenic acid still can not completely replace chemical antibiotics, and the disease resistance is poor.
Hesperidin, also called hesperidin, is a flavonoid substance widely existing in citrus fruits, has a chemical structure of dihydroflavonoid oxyside, is weak acid, and is obtained by extracting to obtain a crude product which is light yellow powder. The pure product is white needle-like crystal and is difficult to dissolve in water. The hesperidin has broad-spectrum antibacterial effect on common food-polluting bacteria, and has remarkable inhibitory effect on bacillus subtilis, salmonella typhimurium, shigella flexneri, streptococcus hemolyticus, vibrio cholerae and the like, so that the hesperidin is widely applied to food additives and food processing.
At present, the report of using tannic acid, chlorogenic acid and hesperidin together as weaned pig additives is not seen, and development is needed.
Disclosure of Invention
The invention aims to solve the technical problems that diarrhea, reduced feed intake and reduced immune function are easy to occur in the existing weaned pigs.
The technical scheme for solving the technical problems is to provide the coated tannic acid preparation for weaned pigs. The coated tannic acid preparation for weaned pigs comprises, by weight, 70-80% of main materials and 20-30% of auxiliary materials, wherein the main materials are mixtures of tannic acid, chlorogenic acid and hesperidin, and the auxiliary materials are mixtures of corn starch, beta-cyclodextrin, sodium alginate, palm oil, acrylic resin and hydroxypropyl methylcellulose acetate succinate.
Further, in the coated tannic acid preparation for weaned pigs, the weight ratio of tannic acid, chlorogenic acid and hesperidin in the main material is 6-10:2-4:0.5-3.
Preferably, in the coated tannic acid preparation for weaned pigs, the weight ratio of tannic acid, chlorogenic acid and hesperidin in the main material is 9:3:2.
Wherein, in the coated tannic acid preparation for weaned pigs, the tannic acid is tannic acid with the purity of more than or equal to 50% extracted from gallnut, tara, chestnut or quebracho.
Wherein, in the coated tannic acid preparation for weaned pigs, the purity of chlorogenic acid is more than or equal to 95 percent.
Wherein, in the coated tannic acid preparation for weaned pigs, the purity of the hesperidin is more than or equal to 20 percent.
In the coated tannic acid preparation for weaned pigs, the weight ratio of corn starch, beta-cyclodextrin, sodium alginate, palm oil, acrylic resin and hydroxypropyl methylcellulose acetate succinate in the auxiliary materials is 7-9:1-4:2-3:3-5:0.5-3:2-6. The preferred weight ratio is 8:2:2:4:1:4.
Wherein, in the coated tannic acid preparation for weaned pigs, the acrylic resin is at least one of acrylic resin I, II and III.
The invention also provides a preparation method of the coated tannic acid preparation for weaned pigs, which comprises the following steps:
a. Weighing beta-cyclodextrin and sodium alginate, dissolving in hot water, adding tannic acid, chlorogenic acid, hesperidin and corn starch, uniformly mixing, and performing spray granulation to obtain microspheres;
b. Heating and melting palm oil, acrylic resin and hydroxypropyl methylcellulose acetate succinate to prepare spray slurry, adding microspheres into a fluidized bed, spraying slurry on the bottom of the fluidized bed at the temperature of 45-60 ℃ for 15-20 minutes, and coating and discharging.
In the preparation method of the coated tannic acid preparation for weaned pigs, the granulating temperature in the step a is 95-110 ℃, and the rotating speed is 600-700 rpm.
In the preparation method of the coated tannic acid preparation for weaned pigs, the particle size of the microspheres in the step a is 90-100 meshes.
The invention also provides application of the coated tannic acid preparation for weaned pigs, and the coated tannic acid preparation is used for preparing an additive or feed for improving the immunity of the weaned pigs.
In the application, the usage amount of the coated tannic acid preparation for the weaned pigs is 200-500 mg/kg of basic ration.
The beneficial effects of the invention are as follows:
The invention provides a coated tannic acid preparation for weaned pigs, which is particularly aimed at the physiological characteristics of weaned pigs, adopts a mode of combining tannic acid, chlorogenic acid and hesperidin, and the hesperidin can neutralize bitter and astringent tastes of tannic acid and chlorogenic acid, improve feed palatability, and can reduce diarrhea rate of weaned pigs, repair damaged intestinal tracts of weaned pigs, maintain microbial cell balance of intestinal tracts and improve disease resistance of weaned pigs by combining the tannic acid, the chlorogenic acid and the hesperidin. Meanwhile, the preparation is coated by a special method, and the bitter taste of tannic acid and chlorogenic acid can be further covered by selecting proper auxiliary materials and coating materials, so that the preparation can be controlled and released slowly, is not degraded in the stomach, and can be degraded directly to the colon part of the small intestine. The coating material adopted by the invention is nontoxic and harmless, can be digested, absorbed and utilized by piglets, and does not influence the absorption of other nutrient elements.
Detailed Description
The invention designs a tannic acid additive special for weaned pigs aiming at the phenomena of easy diarrhea, poor feeding rate, low immune function and the like of the weaned pigs. The conventional tannic acid preparation only adopts tannic acid or coats tannic acid, the bitter taste of the tannic acid can influence the palatability of the feed, the feed intake is reduced, the tannic acid reaching the intestinal tract only depends on astringency, the regulation effect on diarrhea of piglets is realized, and the immunity of the piglets cannot be comprehensively improved.
In order to solve the problems, tannic acid, chlorogenic acid and hesperidin are reasonably matched, the antibacterial and anti-inflammatory effects and good convergence effects of the tannic acid are fully utilized, the intestinal health of piglets is improved, and the diarrhea rate of the piglets is reduced. Meanwhile, chlorogenic acid can promote the expression of intestinal epithelial cells so as to play an antibacterial role, and has a very strong anti-inflammatory effect. Meanwhile, the chlorogenic acid and the hesperidin have antioxidant effect, can maintain the diversity of intestinal microbiota of weaned pigs, cover up the bitter taste of tannic acid and the chlorogenic acid, do not absorb moisture, are not easy to oxidize, and have good product stability, convenient storage and use and good fluidity.
The invention also adopts special coating materials for coating the tannic acid, the chlorogenic acid and the hesperidin, wherein the coating materials are a mixture of corn starch, beta-cyclodextrin, sodium alginate, palm oil, acrylic resin and hydroxypropyl methylcellulose acetate succinate.
Corn starch can improve dispersibility, so that tannic acid, chlorogenic acid and hesperidin are more stable in property and better in dispersibility, and are easier to mix evenly when being mixed in bulk raw materials.
Beta-cyclodextrin, which has a hydrophobic cavity, is capable of forming an inclusion complex, and can reduce oxidation, deactivate photosensitivity and thermosensitive and reduce volatility by forming a complex of tannic acid, chlorogenic acid and hesperidin. Meanwhile, the beta-cyclodextrin can neutralize the bitter taste of tannic acid and improve the palatability of the feed additive.
Sodium alginate is a high-molecular aldehyde acid formed by the aldehyde group of beta-D-mannuronic acid through glycosidic bond, and is a natural macromolecular carbohydrate, and has the advantages of no toxicity, good disintegration effect, good water resistance and stability and easy storage. The enteric-coated tablet has good enteric-coated effect and stable enteric-coated performance, and is suitable for industrial production.
The acrylic resin has the advantages of safety, simple operation, good drying performance, good stability and the like, can reduce the use of a large amount of auxiliary materials, and has good flexibility when the acrylic resin is mixed with hydroxypropyl methylcellulose acetate succinate for use and no plasticizer is added, thereby being suitable for molding.
The hydroxypropyl methylcellulose acetate succinate is used as an enteric coating material and a high polymer carrier, has good film forming property, does not need to add plasticizer, and has good solubility in the upper part of small intestine. Can effectively avoid the degradation of the active ingredients of tannic acid, chlorogenic acid and hesperidin in the stomach, and can be directly re-dispersed to the rear end of the intestine, thereby having good slow-release and controlled-release effects. Meanwhile, the existence of an appropriate amount of acetyl groups in the material can improve the flexibility of a free film of the HPMCAS coating material, improve the acid resistance and prevent materials from agglomerating and bonding. Meanwhile, the existence of a proper amount of succinyl also improves the dissolution performance of the free film of the HPMCAS coating material.
The following examples are provided to further illustrate embodiments of the present invention and are not intended to limit the scope of the invention to the examples.
The raw materials and equipment used in the examples are all common commercial products.
EXAMPLE 1 preparation of coated tannic acid preparation for weaned piglets of the invention
The preparation method comprises the steps of weighing 4.5kg of tannic acid, 1.5kg of chlorogenic acid, 1kg of hesperidin, 1.143kg of corn starch, 0.286kg of beta-cyclodextrin, 0.286kg of sodium alginate, 0.571kg of palm oil, 0.143kg of acrylic resin and 0.571kg of hydroxypropyl methylcellulose acetate succinate.
Dissolving beta-cyclodextrin and sodium alginate in hot water, adding tannic acid, chlorogenic acid, hesperidin and corn starch, uniformly mixing, and performing spray granulation at a temperature of 95 ℃ and a rotating speed of 700rpm to obtain microspheres with a particle size of 90-100 meshes;
Heating and melting palm oil, acrylic resin and hydroxypropyl methylcellulose acetate succinate to obtain slurry, adding the microspheres into a fluidized bed, spraying slurry on the bottom boiling fluidized bed at 45 ℃ for 20 minutes, and coating and discharging.
EXAMPLE 2 preparation of coated tannic acid preparation for weaned piglets of the invention
Respectively weighing 480g of tannic acid, 160g of chlorogenic acid, 160g of hesperidin, 70g of corn starch, 10g of beta-cyclodextrin, 20g of sodium alginate, 30g of palm oil, 10g of acrylic resin and 60g of hydroxypropyl methylcellulose acetate succinate.
Dissolving beta-cyclodextrin and sodium alginate in hot water, adding tannic acid, chlorogenic acid, hesperidin and corn starch, uniformly mixing, and performing spray granulation at 110 ℃ and 600rpm to obtain microspheres with the particle size of 90-100 meshes;
heating and melting palm oil, acrylic resin and hydroxypropyl methylcellulose acetate succinate to obtain slurry, adding the microspheres into a fluidized bed, spraying slurry on the bottom boiling fluidized bed at 60 ℃ for 15 minutes, and coating and discharging.
EXAMPLE 3 preparation of coated tannic acid preparation for weaned piglets of the invention
Respectively weighing 5kg of tannic acid, 1kg of chlorogenic acid, 1.5kg of hesperidin, 0.9kg of corn starch, 0.4kg of beta-cyclodextrin, 0.3kg of sodium alginate, 0.5kg of palm oil, 0.2kg of acrylic resin and 0.2kg of hydroxypropyl methylcellulose acetate succinate.
Dissolving beta-cyclodextrin and sodium alginate in hot water, adding tannic acid, chlorogenic acid, hesperidin and corn starch, uniformly mixing, and performing spray granulation at 100 ℃ at 650rpm to obtain microspheres with the particle size of 90-100 meshes;
Heating and melting palm oil, acrylic resin and hydroxypropyl methylcellulose acetate succinate to obtain slurry, adding the microspheres into a fluidized bed, spraying slurry on the bottom boiling fluidized bed at 50deg.C for 20 min, and coating and discharging.
Comparative example 4 preparation of Single coated tannic acid preparation
Comparative example 4 was divided into 3 groups, 4-1 groups were coated with tannic acid only, 4-2 groups were coated with chlorogenic acid only, 4-3 groups were coated with hesperidin only, tannic acid content of tannic acid group was 4.5kg, acid content of chlorogenic acid group was 1.5kg, and hesperidin content of hesperidin group was 1kg. The components, the amounts and the preparation methods of the adopted auxiliary materials are the same as those of the example 1.
Comparative example 5 preparation of tannic acid formulations using different coating materials
Comparative example 5 was divided into 7 groups, and the coating method was the same as in example 1 except that the main materials were tannic acid 4.5kg, chlorogenic acid 1.5kg, and hesperidin 1 kg.
The coating material formulation and amounts for each group are shown in table 1 below.
TABLE 1 preparation of tannic acid formulations (kg) with different coating materials
Comparative example 6 coating was performed without the coating method of the present invention
The preparation method comprises the steps of weighing 4.5kg of tannic acid, 1.5kg of chlorogenic acid, 1kg of hesperidin, 1.143kg of corn starch, 0.286kg of beta-cyclodextrin, 0.286kg of sodium alginate, 0.571kg of palm oil, 0.143kg of acrylic resin and 0.571kg of hydroxypropyl methylcellulose acetate succinate.
Dissolving beta-cyclodextrin and sodium alginate in hot water, adding tannic acid, chlorogenic acid, hesperidin and corn starch, uniformly mixing, and performing spray granulation at a temperature of 95 ℃ and a rotating speed of 700rpm to obtain microspheres with a particle size of 90-100 meshes;
Heating palm oil, acrylic resin and hydroxypropyl methylcellulose acetate succinate for melting, mixing the melted liquid with the microspheres, freezing and spraying, and coating for discharging.
Test example 7 stability analysis of each coated tannic acid preparation
And (3) simulating pig stomach and intestine digestion in vitro by using a dissolution tester, and measuring the digestive tract release amount of the tannic acid preparation by taking the accumulated release percentage of the in vitro release degree as an evaluation index.
The gastrointestinal dissolution rates of the respective examples and comparative examples are shown in table 2 below.
TABLE 2 gastrointestinal cumulative Release Rate (%)
| Stomach 1h | Stomach 2h | Stomach 4h | Intestine 1h | Intestine 2h | Intestine 4h | Intestine 8h | |
| Example 1 | 0.35 | 1.24 | 2.76 | 15.44 | 25.74 | 55.83 | 77.53 |
| Example 2 | 0.33 | 1.57 | 2.53 | 13.12 | 24.93 | 53.11 | 75.13 |
| Example 3 | 0.46 | 1.42 | 2.90 | 14.97 | 23.22 | 55.12 | 75.03 |
| Comparative example 4-1 | 0.38 | 1.33 | 2.87 | 15.11 | 26.12 | 54.17 | 72.10 |
| Comparative example 4-2 | 0.39 | 1.45 | 2.90 | 16.23 | 25.95 | 57.12 | 74.18 |
| Comparative examples 4 to 3 | 0.41 | 1.49 | 2.56 | 15.60 | 26.74 | 57.29 | 69.66 |
| Comparative example 5-1 | 1.39 | 1.98 | 3.54 | 18.23 | 23.30 | 51.11 | 71.24 |
| Comparative example 5-2 | 0.35 | 1.76 | 2.69 | 15.74 | 22.78 | 49.48 | 72.02 |
| Comparative examples 5 to 3 | 0.37 | 1.43 | 2.73 | 15.98 | 25.30 | 54.89 | 75.11 |
| Comparative examples 5 to 4 | 3.45 | 12.09 | 22.23 | 34.89 | 42.81 | 64.02 | 72.10 |
| Comparative examples 5 to 5 | 3.78 | 11.75 | 23.04 | 35.01 | 44.67 | 65.09 | 73.23 |
| Comparative examples 5 to 6 | 1.34 | 5.78 | 13.22 | 25.20 | 38.12 | 56.39 | 67.19 |
| Comparative examples 5 to 7 | 7.35 | 15.83 | 33.02 | 44.24 | 56.12 | 63.45 | 68.12 |
| Comparative example 6 | 2.98 | 4.23 | 10.55 | 34.13 | 41.23 | 54.24 | 63.92 |
As shown in the experimental results of Table 2, the coating agents and the coating method of the invention are adopted in examples 1-3 and comparative example 4, so that extremely low dissolution rate in the stomach can be basically ensured, and the dissolution rate in the intestinal tract is over 70% after continuous disintegration, and the dissolution effect is good. In comparative example 5, since the coating materials were selected in different compositions and ratios, a part of the group was dissolved in the stomach, and the effective functional components reaching the intestinal tract were reduced. Especially, the degradation rate in the stomach is high and reaches 33.02%, and when any one of acrylic resin or hydroxypropyl methylcellulose acetate succinate is absent in the coating material, the dissolution rate in the stomach is also greatly improved, so that the coating material disclosed by the invention can have good effect of not degrading in the stomach and degrading intestinal tracts only when the coating material is used according to the formula amount by requiring specific components. The results of comparative example 6 also show the importance of the coating method, which also affects the gastrointestinal dissolution of the active ingredient, and the method of the present invention can reduce the dissolution rate in the stomach more than other coating methods.
Test example 8 influence of each coated tannic acid preparation on weaned pigs
70 Healthy three-way-bred 30-day-old weaned pigs were selected, initially weighing (8.97+ -1.48) kg, randomly divided into 14 groups of 5 replicates each, and 1 pig each replicate. The coated tannic acid preparations prepared in examples 1 to 3 and comparative examples 4 to 6 were added to daily ration, respectively, and 250mg/kg of the coated tannic acid preparation was added to the daily ration based on the feeding amount.
The test period is 33d, wherein the pre-test period is 5d. Adopts single-column feeding, and can freely feed and drink water. The test ration adopts common commercial weaned piglet basic ration.
The daily feeding amount and the residual amount are accurately recorded, and the 8:00 piglet in the morning is weighed on a hollow basis on the 1 st day to the 28 th day of the formal experiment. Each group was collected from the anterior vena cava, anticoagulated with heparin sodium, and centrifuged at 4 ℃ and 3000r/min for 20min to separate plasma. The visceral tissues were removed by slaughter after blood sampling, and the lengths of jejunum and ileum and colon were measured.
Growth performance was calculated according to the following formula average daily gain = full phase gain/day; average daily feed intake = full phase feed intake/day.
The effect of tannic acid formulation of the present invention on growth performance of weaned pigs is shown in table 3 below.
TABLE 3 Effect of tannic acid formulations on growth performance of weaned piglets
From the results shown in Table 3, it is clear that the feeding of the compound tannic acid preparation of the invention can more effectively improve the feed intake and daily gain of weaned pigs and improve the growth performance of weaned pigs compared with the feeding of the preparation with single component and the feeding of the compound preparation with other coating components in comparative example 4. In the experimental group fed with the compound tannic acid preparation, the diarrhea rate of piglets is also obviously reduced, diarrhea piglets basically do not appear, and in the preparation group fed with a single component, diarrhea of piglets still appears.
According to the kit, the activities of total antioxidant capacity (T-AOC), total superoxide dismutase (T-SOD), catalase (CAT), glutamic pyruvic transaminase (ALT) and glutamic oxaloacetic transaminase (AST) in blood plasma are determined by adopting a colorimetric method. The effect of tannic acid formulation of the present invention on plasma biochemical index of weaned pigs is shown in table 4 below.
TABLE 4 influence of tannic acid formulation on plasma Biochemical index of weaned piglets (U/ml)
From the results shown in Table 4, it is apparent that the compound preparation of the present invention can significantly enhance the antioxidant function of piglet plasma compared with the single use of tannic acid, chlorogenic acid or hesperidin. The possible reason for enhancing the oxidation resistance of piglets is that the feed intake of piglets can be improved by adding the tannic acid, chlorogenic acid and hesperidin composite preparation, enough protein and energy are taken in to promote the growth and development of organisms, in addition, chlorogenic acid and hesperidin have the oxidation resistance function and are matched for use, and the tannic acid is combined, so that the oxidation resistance of organisms can be improved, the lipid reaction level of cell membranes can be reduced, the integrity of cell membranes can be protected, the normal liver function can be maintained, and ALT and AST can be reduced from escaping from tissue cells.
The effect of the tannic acid preparation of the present invention on the morphology of intestinal mucosa tissue of weaned pigs is shown in table 5 below.
TABLE 5 influence of tannic acid formulation on intestinal mucosal tissue morphology (um) of weaned pigs
| Jejunum villus height | Ileum villus height | Height of colon villus | |
| Example 1 | 305 | 296 | 231 |
| Example 2 | 311 | 287 | 227 |
| Example 3 | 308 | 298 | 229 |
| Comparative example 4-1 | 265 | 244 | 198 |
| Comparative example 4-2 | 254 | 253 | 195 |
| Comparative examples 4 to 3 | 233 | 245 | 201 |
| Comparative example 5-1 | 287 | 265 | 211 |
| Comparative example 5-2 | 285 | 271 | 205 |
| Comparative examples 5 to 3 | 292 | 267 | 206 |
| Comparative examples 5 to 4 | 279 | 262 | 212 |
| Comparative examples 5 to 5 | 276 | 271 | 215 |
| Comparative examples 5 to 6 | 278 | 267 | 216 |
| Comparative examples 5 to 7 | 241 | 255 | 199 |
| Comparative example 6 | 287 | 251 | 215 |
As shown in Table 5, the compound tannic acid preparation of the present invention can increase the villus height of jejunum, ileum and colon of piglets, promote intestinal villus development and reduce villus damage. The result shows that the compound tannic acid preparation has the effect of relieving the intestinal stress response of the weaned piglets. The action mechanism is possibly related to the antioxidation function, and the oxidation damage to the intestinal tract is avoided by reducing the organism oxidation stress level, so that the intestinal tract development is promoted, and the integrity of the morphological structure of the intestinal tract is improved.
In conclusion, the coated tannic acid preparation for weaned pigs designed by the invention can avoid degradation in stomach, has good dissolution rate of active ingredients in intestinal tracts, can promote weaned pigs to increase feed intake and daily gain, enhance growth performance, improve antibacterial capability and antioxidant level, reduce oxidative damage of intestinal tracts, improve morphological and structural integrity of intestinal tracts, keep organisms healthier, and has good practical significance.
Claims (7)
1. The coated tannic acid preparation for weaned pigs is characterized by comprising, by weight, 70-80% of main materials and 20-30% of auxiliary materials, wherein the main materials are a mixture of tannic acid, chlorogenic acid and hesperidin, the weight ratio of tannic acid to chlorogenic acid to hesperidin in the main materials is 6-10:2-4:0.5-3, the auxiliary materials are a mixture of corn starch, beta-cyclodextrin, sodium alginate, palm oil, acrylic resin and hydroxypropyl methylcellulose acetate succinate, and the weight ratio of corn starch, beta-cyclodextrin, sodium alginate, palm oil, acrylic resin and hydroxypropyl methylcellulose acetate succinate in the auxiliary materials is 7-9:1-4:2-3:3-5:0.5-3:2-6, and the preparation method of the coated tannic acid preparation for weaned pigs comprises the following steps:
a. Weighing beta-cyclodextrin and sodium alginate, dissolving in hot water, adding tannic acid, chlorogenic acid, hesperidin and corn starch, uniformly mixing, and performing spray granulation to obtain microspheres;
b. Heating and melting palm oil, acrylic resin and hydroxypropyl methylcellulose acetate succinate to prepare spray slurry, adding microspheres into a fluidized bed, spraying slurry on the bottom of the fluidized bed at the temperature of 45-60 ℃ for 15-20 minutes, and coating and discharging.
2. The coated tannic acid preparation for weaned pigs according to claim 1, wherein the weight ratio of tannic acid, chlorogenic acid and hesperidin in the main material is 9:3:2.
3. The coated tannic acid preparation for weaned pigs according to claim 1, wherein the tannic acid is tannic acid with a purity of not less than 50% obtained by extraction of gallnut, tara, chestnut or quebracho, the chlorogenic acid has a purity of not less than 95%, and the hesperidin has a purity of not less than 20%.
4. The coated tannic acid preparation for weaned pigs according to claim 1, wherein the weight ratio of corn starch, beta-cyclodextrin, sodium alginate, palm oil, acrylic resin and hydroxypropyl methylcellulose acetate succinate in the auxiliary materials is 8:2:2:4:1:4.
5. The coated tannic acid preparation for weaned pigs according to claim 1, wherein the granulating temperature in the step a is 95-110 ℃ and the rotating speed is 600-700 rpm.
6. The coated tannic acid preparation for weaned pigs according to claim 1, wherein the particle size of the microspheres in the step a is 90-100 meshes.
7. Use of the coated tannic acid preparation for weaned pigs according to any one of claims 1-6 for preparing an additive or feed for improving immunity of weaned pigs.
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| CN107125440A (en) * | 2016-02-29 | 2017-09-05 | 湖南晶天科技实业有限公司 | A kind of feed addictive enteric coating tannic acid and preparation method thereof and a kind of feed |
| CN108077179A (en) * | 2017-12-29 | 2018-05-29 | 霍邱县运发牧业有限责任公司 | A kind of method for breeding for improving piglet survival ratio |
| CN115227696A (en) * | 2022-07-29 | 2022-10-25 | 广州傲农生物科技有限公司 | Compound acidifier for resisting diarrhea and promoting growth of piglets as well as preparation method and application thereof |
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| US20180133243A1 (en) * | 2016-11-16 | 2018-05-17 | Holista Colltech Ltd | Method and composition for crude formulations of fortified sugar for glycemic control |
| CN111248351A (en) * | 2020-03-21 | 2020-06-09 | 天津瑞益瑞美生物技术有限公司 | Coated compound acidifier and preparation method thereof |
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| CN107125440A (en) * | 2016-02-29 | 2017-09-05 | 湖南晶天科技实业有限公司 | A kind of feed addictive enteric coating tannic acid and preparation method thereof and a kind of feed |
| CN108077179A (en) * | 2017-12-29 | 2018-05-29 | 霍邱县运发牧业有限责任公司 | A kind of method for breeding for improving piglet survival ratio |
| CN115227696A (en) * | 2022-07-29 | 2022-10-25 | 广州傲农生物科技有限公司 | Compound acidifier for resisting diarrhea and promoting growth of piglets as well as preparation method and application thereof |
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