CN116172196A - A health drink containing broad bean (with pods) extract - Google Patents

Abstract

The invention discloses a health drink for improving Parkinson's syndrome, the active ingredient is broad bean (with pods) extract and natural plant mixed extract, the ratio of the two is (1‑8):1; natural plant mixed The composition of the extract is as follows by weight: 10-15 parts of Tianma, 10-15 parts of Astragalus, 5-10 parts of Angelica, 2-3 parts of Chuanxiong, 3-5 parts of Magnolia officinalis, 5-10 parts of Poria cocos, 1-2 parts of licorice Compared with the prior art, the health drink prepared by the present invention has a synergistic effect after the increased natural plant combination extract is combined with the broad bean (with pods) extract, which makes up for the single function of the active ingredient of the single broad bean extract. It is more suitable for the health care needs of the Parkinson's population.

Description

A health drink containing broad bean (with pods) extract

Technical Field

The invention relates to health care product technology, and in particular to a health care beverage containing broad bean (with pods) extract and a preparation method thereof.

Background Art

In recent years, there have been more and more studies on the physiological activities of L-dopamine, proanthocyanidins and flavonoids extracted from broad beans. The proanthocyanidins have antioxidant, antibacterial, anti-cancer and anti-mutagenic physiological activities. Flavonoids also have lipid-lowering, anti-atherosclerotic, anti-tumor and anti-osteoporosis effects. People have always had the habit of eating broad beans to relieve Parkinson's disease.

However, a single broad bean extract health product has a single effect and cannot provide a relatively comprehensive health function. The present invention adds a variety of natural substances that are both medicinal and edible. Through the synergistic effect of natural ingredients, the efficacy range of broad bean extract health products is expanded, providing Parkinson's patients with more abundant choices.

Summary of the invention

The object of the present invention is achieved in this way.

A health drink for improving Parkinson's syndrome, wherein the active ingredients are broad bean (with pod) extract and natural plant mixed extract.

The plant components in the natural plant mixed extract are as follows by weight: 10-15 parts of Gastrodia elata, 10-15 parts of Astragalus membranaceus, 5-10 parts of Angelica sinensis, 2-3 parts of Chuanxiong, 3-5 parts of Magnolia officinalis, 5-10 parts of Poria cocos, and 1-2 parts of Radix Glycyrrhizae Preparata.

The preparation method of the health drink containing broad bean (with pods) extract is as follows:

The preparation method of broad bean (with pods) extract is as follows: fresh broad beans (with pods) are screened and weighed, washed and boiled with 1-2 times the amount of water for 1 minute, filtered to remove the water and then squeezed the juice, and the juice was filtered with a 400-mesh filter cloth. 0.01%-0.05% sodium pyrosulfite and 0.05-0.2% disodium edetate are added to the filtrate to obtain the broad bean (with pods) extract.

The preparation method of other natural plant mixed extracts is as follows: add the formula amount of Gastrodia elata, Astragalus, Angelica sinensis, Chuanxiong, Magnolia officinalis, Poria cocos, and Glycyrrhiza uralensis to the extraction tank, add 5-10 times the total weight of the above plants in water, soak for 0.5-1.0 hours, boil the soaked plants and start timing, boil for 2 hours and release the boiling liquid; then add 5-10 times the total amount of food water for the plants, continue to boil for 90 minutes, and release the boiling liquid. Combine the boiling liquids, filter with gauze, and take the filtrate and decompress and concentrate it to an extract with a density of 1.1-1.3.

Broad bean (with pods) extract and other natural plant mixed extracts are mixed in a certain ratio of (1-8):1, 0.05%-0.1% acesulfame potassium and 0.05%-0.1% potassium sorbate are added, and the mixture is instantaneously killed and then filled to obtain a health beverage.

Parkinson's disease belongs to the category of "tremor syndrome" in traditional Chinese medicine. The disease is located in the brain and involves the liver, kidney and spleen. Traditional Chinese medicine believes that the pathogenesis of this disease is liver and kidney deficiency and insufficient qi and blood. Generally, the method of nourishing the liver and kidney, replenishing qi and blood, and extinguishing wind and stopping tremor is adopted.

In the present invention, Gastrodia elata is the main drug, which has the effects of calming wind and stopping spasm, calming liver yang, and dispelling wind and unblocking collaterals. Astragalus is the assistant drug, which invigorates qi and consolidates the exterior, supports toxins and discharges pus. Magnolia officinalis, Poria cocos and Chuanxiong are adjuvant drugs. Poria cocos strengthens the spleen and earth, helps the acquired qi and blood to form, Magnolia officinalis regulates qi and widens the intestines, prevents qi stagnation, and Chuanxiong activates blood circulation and promotes qi, can open and disperse, ascends to the head, reaches the limbs, descends to the sea of blood, and can promote qi and blood. The three work together to make qi and blood flow and the meridians unblocked. Radix Glycyrrhizae Preparata is the guiding drug, which harmonizes various drugs and invigorates the spleen and replenishes qi. After the natural plant combination extract is combined with the broad bean (with pods) extract, a synergistic effect is exerted, which makes up for the single function of the effective ingredient of the single broad bean extract, and is more suitable for the health care needs of Parkinson's disease population.

DETAILED DESCRIPTION

In order to better illustrate the purpose, technical solutions and advantages of the present invention, the present invention will be further described below in conjunction with specific embodiments.

Example 1: Preparation of health beverage

The preparation method of broad bean (with pods) extract is as follows: fresh broad beans (with pods) are screened and weighed to 16.3 kg, washed and boiled with 1-2 times the amount of water for 1 minute, filtered to remove the water and then squeezed the juice, and the juice was filtered with a 400-mesh filter cloth. 0.01% sodium pyrosulfite and 0.2% disodium edetate were added to the filtrate to obtain the broad bean (with pods) extract.

The preparation method of other natural plant mixed extracts is as follows: add 12 parts of Gastrodia elata, 12 parts of Astragalus, 8 parts of Angelica sinensis, 2 parts of Chuanxiong, 3 parts of Magnolia officinalis, 5 parts of Poria cocos, and 1 part of Glycyrrhiza uralensis to an extraction tank, add 8 times the total weight of the above plants in water, soak for 0.5 hours, boil the soaked plants and start timing, boil for 2 hours and release the boiled liquid; then add 5 times the total amount of food water for the plants, continue to boil for 90 minutes, and release the boiled liquid. Combine the boiled liquids, filter with gauze, and take the filtrate and concentrate it under reduced pressure to an extract with a relative density of 1.17 at 25 degrees Celsius.

Take 4 parts of broad bean (with pods) extract and 1 part of other natural plant mixed extracts, add 0.05% acesulfame potassium and 0.05% potassium sorbate, kill at 130°C for 30 seconds and then fill into 50ml/bottle to obtain a health drink containing broad bean (with pods) extract.

Example 2: Pharmacodynamics Experiment

Thirty adult healthy mice were divided into three groups: blank group, model group, and experimental group, with 10 mice in each group.

Experimental grouping: 30 adult mice were divided into 3 groups, 10 mice in each group, blank control group (normal saline gavage for 1-21 days, normal saline intraperitoneal injection for 22-29 days), model group (normal saline gavage for 1-21 days, MPTP (Note: MPTP is 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine, the same below) for 22-29 days), drug administration group (health drink of the present invention for 1-21 days, MPTP for 22-29 days)

Dosage and administration method: Normal saline (oral gavage/intraperitoneal injection): 30 mg/kg/d

Health care beverage (intragastric administration): 1 ml/kg, the beverage was prepared as in Example 1.

MPTP (intraperitoneal injection): 30 mg/kg/day

Dosing frequency: Once a day

Behavioral testing methods:

Pole climbing test: Take a smooth wooden pole with a diameter of 1 cm, a height of 50 cm, and a wooden ball with a diameter of 3 cm on the top. During the test, the mice were placed on the cork ball, and the time required for the mice to climb to the bottom of the pole was recorded and statistically analyzed. The climbing time of all mice was measured on the 21st day of drug administration, and the climbing time was measured again 1 hour after drug administration on the 29th day.

Rotating axis test: Place mice on a rotating rod and test the time from the start of the rotation of the rod to the time the mice fall off the rod. The rotating axis time of all mice was measured on the 21st day of administration, and on the 29th day, 1 hour after administration, their rotating axis time was measured.

Grip strength test: Place the test mice on a tensile tester, grab the tail of the mouse, pull it backward in a straight line, and record the grip strength data. Measure the grip strength of all mice on the 21st day of administration, and measure the pulling force again on the 29th day after administration.

Efficacy results:

The data show that: through the mouse Parkinson's model, the drug group can significantly increase the climbing time and rotation time of the model group mice, and increase the pulling force of the mice. This shows that the health drink has a significant therapeutic effect on Parkinson's model mice.

Example 3: Pharmacodynamics Experiment

Thirty healthy adult mice were divided into three groups: blank group, model group, and experimental group, with 10 mice in each group.

Experimental groups: 30 adult mice were divided into 3 groups, 10 mice in each group, blank control group (normal saline gavage for 1-21 days, normal saline intraperitoneal injection for 22-21 days), model group 1 (normal saline gavage for 1-21 days, arecoline modeling), model group 2 (normal saline gavage for 1-21 days, oxotremorine modeling), drug administration group (health drink of the present invention for 1-21 days, arecoline modeling).

Dosage and administration method: Normal saline (oral gavage/intraperitoneal injection): 30 mg/kg/d

Health care beverage (intragastric administration): 1 ml/kg, the beverage was prepared as in Example 1.

Arecoline (intraperitoneal injection): 30 mg/kg/day

Oxotremorine (intraperitoneal): 0.30 mg/kg/day

Dosing frequency: Once a day

Behavioral detection: The time required for each mouse from the onset of muscle tremor that is clearly visible to the naked eye to its disappearance is recorded as the tremor duration.

Efficacy results:

The data show that mice developed obvious tremors after intraperitoneal injection of arecoline and oxidotremorine, and the tremor duration of the arecoline group was longer. However, the groups given the health drink prepared by the present invention in advance all had significantly shortened tremor duration, indicating that the health drink of the present invention has a significant improvement effect on the tremor formed by simulating Parkinson's disease. It has a relieving effect on the tremor formed by the arecoline modeling and the tremor formed by the oxidotremorine modeling, indicating that the health drink has a significant therapeutic effect on Parkinson's disease model mice.

Claims (5)

1. A health drink for improving Parkinson's syndrome, characterized in that the active ingredient is broad bean (with pods) extract and natural plant mixed extract. 2. The health drink for improving Parkinson's syndrome according to claim 1, characterized in that, the plant components in the natural plant mixed extract are as follows by weight: 10-15 parts of Gastrodia elata, 10-15 parts of Radix Astragali , Angelica 5-10 parts, Chuanxiong 2-3 parts, Magnolia officinalis 3-5 parts, Poria cocos 5-10 parts, licorice 1-2 parts. 3. The health drink for preventing and treating Parkinson's syndrome according to claim 1, characterized in that the weight ratio of broad bean (with pods) extract and other natural plant mixed extracts is (1-8):1. 4. The health drink for preventing and treating Parkinson's syndrome according to claim 1 is characterized in that the preparation method is as follows: the preparation method of the broad bean (with pods) extract is as follows: fresh broad beans (with pods) are screened and weighed Heavy, after cleaning, boil with 1-2 times the amount of water for 1min, filter the water and squeeze the juice, filter the juice with a 400-mesh filter cloth, add 0.01%-0.05% sodium metabisulfite, 0.05-0.2% Disodium dioic acid, to obtain broad bean (containing pods) extract; the preparation method of the natural plant mixed extract is as follows: Gastrodia elata, astragalus, angelica, Chuanxiong, Magnolia officinalis, Poria cocos, licorice are added in the extraction tank, and the above-mentioned 5-10 times the amount of water of the total plant weight, soak for 0.5-1.0 hours, start timing after the soaked plants are boiled, and decoct for 2 hours to release the cooking liquid; then add food water of 5-10 times the total amount of plants, Continue to decoct for 90 minutes, release the cooking liquid; combine the cooking liquid, filter it with gauze, take the filtrate and concentrate it under reduced pressure to an extract with a relative density of 1.1-1.3 at 25 degrees Celsius; take broad bean (with pods) extract and other natural plants for mixed extraction Mix the ingredients, add 0.05%-0.1% acesulfame-K, 0.05%-0.1% potassium sorbate, fill it after killing instantly, and obtain the health product beverage. 5. according to claim 1, be used for preventing and treating the health drink of Parkinson's syndrome, it is characterized in that, the weight ratio of broad bean (bean pod) extract and other natural plant mixed extracts described in the preparation method is (1-8 ):1.