CN116036384A - Preparation method of self-oxygenated antibacterial filling and fixing material - Google Patents

Abstract

The invention discloses a preparation method of a self-oxygen-supplying antibacterial filling fixing material, which comprises the following steps: 1) Dissolving the medicine in hydrogen peroxide solution, namely solution A; preparing polyethylene glycol solution, namely solution B; preparing a catalase solution, namely a solution C; 2) Respectively adding montmorillonite into the solution A, the solution B and the solution C, stirring, filtering and vacuum drying; repeating for multiple times to obtain a hydrogen peroxide/antibiotics zero-order release carrier; 3) Preparing a filling fixing material component D; 4) Preparing a filling fixing material component E; 5) Mixing the filling and fixing material component D and the filling and fixing material component E, stirring, and filling the open fracture part by injection. The method can fully fill and fix the wound part, prevent infection or treat the wound part in an antibacterial way, and avoid secondary damage of the wound part caused by external factors during first-aid transportation.

Description

Preparation method of self-oxygenated antibacterial filling and fixing material

technical field

The invention belongs to the technical field of preparation of biomedical materials, and relates to a preparation method of a self-oxygenation antibacterial filling and fixing material.

Background technique

At present, the number of patients with open trauma caused by various natural disasters is increasing. Although they have received rich medical technical support in the later stage of treatment, traditional splint external fixation is still mainly used in the pre-hospital emergency period to fix the wound internally. The effect is very small and the fixation efficiency is low, which may even lead to secondary trauma or other problems, and then aggravate the injury. There are certain shortcomings, and first aid is especially important for post-trauma treatment. Pre-emergency, reducing the probability of side effects during the first aid period, improving the effect of later treatment, and solving the problems of trauma pre-hospital first aid techniques are urgently needed to be overcome. After the trauma occurs, due to environmental reasons, it is easy to cause bacterial infection at the wound site. If antibacterial treatment is not carried out in time, the wound site will develop into a purulent wound. If the situation is more serious, it will be shocked and amputated, and even the spread of severe bacterial infection will cause death. Therefore, The material needs to have an antibacterial effect. In addition, the most important thing is that the patient needs to ensure that the fixed part has a certain amount of oxygen during the transfer, so as to avoid the accelerated bacterial proliferation and the anoxic death of normal cells caused by the lack of oxygen content after filling and fixing. Therefore, the development of a A self-oxygenated antibacterial material that can be used for filling and fixing wound sites becomes the key to solving the above problems.

The Chinese invention patent "A Slow-release Oxygen Microsphere and Its Preparation Method, Application Method and Application in the Treatment of Diabetic Foot and Tumor Diseases" (application number 202210868565.6, publication date 2022.10.21, publication number CN 115212183A) discloses a Slow-release oxygen microspheres and their preparation method, use method and application in the treatment of diabetic foot and tumor diseases, including an inner core and a shell wrapped outside the inner core, the inner core contains H ₂ O ₂ solution dissolved with CaCl ₂ and PVP, and the outer shell Including hydrogel and catalase and stem cells adhered to the surface of the hydrogel, the hydrogel uses sodium alginate, agarose, hyaluronic acid (HA), chitosan, carrageenan (CRG), Gellan gum (GG), a kind of gellan gum (GG), has stable oxygen release performance, high loading capacity, and low loss rate, which can effectively solve the disadvantage that the amount of oxygen in the release process is difficult to control, thereby slowly releasing oxygen in an anoxic microenvironment and improving The therapeutic effect of stem cells. However, although the oxygen-releasing microspheres have the effect of controlling oxygen release, there is an initial small burst release phenomenon, which easily causes cellular oxygen poisoning. Chinese invention patent "A Slow and Controlled Oxygen Release Microsphere and Its Preparation Method and Application" (application number 201810174515.1, publication date 2018.07.24, publication number CN 108310470 A) discloses a slow and controlled oxygen release microsphere and its preparation method and purposes, the slow and controlled oxygen release microspheres are gelatin-based microspheres, containing peroxide inorganic particles inside the microspheres, and the peroxide inorganic substances are selected from CaO ₂ , MgO ₂ , NaCO ₃ , SrO ₂ , BaO _2. K ₂ O ₂ , Na ₂ O ₂ . The disclosed slow-controlled oxygen release material has the advantages of controllable size, long oxygen release time, controllable oxygen release time, low burst release rate, low cytotoxicity, low immunogenicity, relatively simple preparation process, and easy industrialization. The clinical application of bone defect repair has great application prospects, but the microspheres still have a short-term burst drug release phenomenon, which is easy to cause cellular oxygen poisoning in the initial oxygen supply stage.

Due to the negative effects of burst release, such as high local concentration and insufficient release concentration in the later period, it is impossible to maintain a stable therapeutic effect. Therefore, an oxygen release carrier with an accurate zero-order release kinetic model was designed to avoid the negative effects of burst release. The side effects make sense. In addition, in order to meet the good antibacterial effect, the ideal drug release should also meet the zero-order release model to avoid side effects caused by drug burst release. Therefore, based on the current emergency needs, it is urgent to prepare an ideal zero-order release oxygen-supplying antibacterial open wound internal filling and fixing emergency material, which has great clinical application value.

Contents of the invention

The purpose of the present invention is to provide a preparation method of a self-oxygenated antibacterial filling and fixing material, which solves the problem of anti-infection treatment and improvement caused by the unsatisfactory filling and fixing first aid material used in the wound site during the pre-hospital first aid of trauma in the prior art. And the problem of insufficient first aid effect.

The technical solution adopted in the present invention is a preparation method of a self-oxygenated antibacterial filling and fixing material, which is specifically implemented according to the following steps:

Step 1, preparing a hydrogen peroxide solution, dissolving the drug in the hydrogen peroxide solution according to the mass volume percentage of 5-15 mg/ml, referred to as solution A; preparing a polyethylene glycol solution, referred to as solution B; preparing a catalase solution, referred to as solution C;

Step 2, first add montmorillonite to solution A, after ultrasonic dispersion, carry out stirring with hydrogen peroxide and drug loading, after the stirring is completed, suction filter, and vacuum dry; then redisperse the dried montmorillonite in solution B , carry out PEG coating and stirring, suction filtration after stirring, and vacuum drying; finally, redisperse the dried montmorillonite in solution C, carry out stirring with catalase, suction filtration after stirring, and vacuum drying; repeat This step is repeated multiple times to obtain a zero-order release carrier loaded with hydrogen peroxide/antibiotics;

Step 3, mix the PMMA powder, the initiator BPO, and the hydrogen peroxide/antibiotic zero-order release carrier obtained in step 2 to obtain the filling and fixing material component D;

Step 4, uniformly mixing MMA, N,N-dimethyl-p-toluidine, and hydroquinone to obtain the filling and fixing material component E;

Step 5, mix the filling and fixing material component D obtained in step 3 with the filling and fixing material component E obtained in step 4, stir, and fill the open fracture site by injection, the filling and fixing material component D and the filling and fixing material component E After the reaction is over, it's done.

The beneficial effects of the present invention include the following advantages:

1) Using montmorillonite with water absorption as the matrix, hydrogen peroxide, antibiotics, PEG and catalase are loaded on the surface of montmorillonite by layer-by-layer self-assembly technology. First of all, the water absorption of montmorillonite can ensure that the material absorbs blood tissue fluid and then expands during the filling process, so that the PMMA material expands, and the compressive stress generated by the expansion makes the filling more compact and compresses the surrounding bleeding tissue to stop bleeding, achieving dual filling and hemostasis. Purpose; secondly, the layer-by-layer self-assembly technology relies on dynamic hydrogen bonds to compound all substances. Hydrogen peroxide and antibiotics are released depending on the concentration difference. When hydrogen peroxide is released, it is decomposed by catalase to generate oxygen. Oxygen supply ensures sufficient oxygen content in the wound site, prevents the growth and reproduction of various bacteria, and keeps the wound part dry; in addition, the self-oxygen supply function can make tissue cells receive more sufficient oxygen and avoid cell hypoxic death during fixation , At the same time, the release of antibiotics can carry out broad-spectrum antibacterial on the wound site, avoiding or performing prophylactic or antibacterial treatment on the wound site.

2) The prepared oxygen/antibiotic release carrier is a multilayer self-assembly based on dynamic hydrogen bonds. The release rate of hydrogen peroxide and antibiotics is related to the disintegration of hydrogen bonds between layers. At the same time, the molecular weight of PEG is used as the disintegration rate decision switch to make The disintegration of hydrogen bonds is controlled, thereby controlling the release of drugs. At the same time, the disintegration rate of PEG is constant, and finally the release of oxygen and antibiotics is zero-order release and is not affected by other factors.

3) The PMMA fixation material with good biocompatibility is used as the matrix. On the one hand, it is because of its good biocompatibility, which ensures the safety of human tissue during the filling of the wound site. Secondly, the PMMA material has certain plasticity. It can be shaped arbitrarily according to the wounded part. The PMMA material is self-curing and has high mechanical properties after curing. After being fully fixed, it can protect the filling part and avoid secondary damage caused by other reasons during transportation.

Detailed ways

The preparation method of the self-oxygenation antibacterial filling and fixing material of the present invention is implemented according to the following steps:

Step 1, prepare hydrogen peroxide solution, dissolve the drug in the hydrogen peroxide solution according to the mass volume percentage of 5-15 mg/ml, referred to as solution A; prepare polyethylene glycol (PEG) solution, referred to as solution B; prepare catalase solution, referred to as solution B; Solution C;

The volume fraction of the hydrogen peroxide solution is 20-40%, the concentration of the polyethylene glycol (PEG) solution is 75-115 mg/ml, and the concentration of the catalase solution is 100-400 μg/ml;

The drug is one of vancomycin hydrochloride, levofloxacin hydrochloride, tetracycline hydrochloride or ceftriaxone sodium;

PEG selects one of PEG2000, PEG4000, PEG6000, PEG8000 or PEG10000.

Step 2, first add montmorillonite to solution A, after ultrasonic dispersion, carry out stirring with hydrogen peroxide and drug loading, after stirring, filter with suction and vacuum dry; then redisperse the once-dried montmorillonite in solution B PEG-coated stirring, after the stirring, suction filtration, and vacuum drying; finally, the secondary dried montmorillonite was redispersed in solution C, carrying out stirring with catalase, after the stirring, suction filtration, vacuum drying Drying; repeat this step several times, and use the layer-by-layer self-assembly technology to obtain the hydrogen peroxide/antibiotic zero-order release carrier;

The quality of montmorillonite is 20-40% of the volume of solution A, the time for hydrogen peroxide and drug loading and stirring is 1-2 hours, the time for PEG coating and stirring is 1-2 hours, and the time for carrying catalase is 1-2 hours. ～2h, the number of repeated cycles is 3～8 times.

Step 3, mix the PMMA powder, the initiator BPO, and the hydrogen peroxide/antibiotic zero-order release carrier obtained in step 2 to obtain the filling and fixing material component D for emergency use;

Among them, the mass ratio of the PMMA powder, the initiator BPO and the hydrogen peroxide/antibiotic zero-order release carrier is 49.95-69.85:0.05-0.15:30-50.

Step 4, mixing MMA, N,N-dimethyl-p-toluidine, and hydroquinone evenly to obtain the filling and fixing material component E for emergency use;

Wherein, the mass ratio of MMA, N,N-dimethyl-p-toluidine and hydroquinone is 96.5-99.5:0.3-3:0.1-0.5.

Step 5, mix the filling and fixing material component D obtained in step 3 with the filling and fixing material component E obtained in step 4 according to a solid-liquid ratio of 1 to 2 g/ml, stir, and fill the open fracture site by injection. After the reaction between component D of the fixing material and component E of the filling and fixing material is completed, the wound site can be further fixed.

The preparation method of the self-oxygenation antibacterial filling and fixing material of the present invention first adopts the dynamic hydrogen bond layer-by-layer self-assembly technology to load hydrogen peroxide, antibiotics, PEG and catalase on montmorillonite to prepare a zero-order release carrier, and then PMMA powder, initiator BPO, and hydrogen peroxide/antibiotic zero-order release carrier are mixed evenly to obtain filling and fixing material component D, and MMA, N,N-dimethyl-p-toluidine, and hydroquinone are mixed evenly to get filling and fixing Material component E, and finally mix the two components to obtain a zero-order release oxygen-supplying antibacterial internal filling and fixing first aid material for open fractures. The open fracture can be fully filled by injection packing, and the filling part can be fixed after PMMA hardens .

Example 1

The preparation method of the self-oxygen supply antibacterial filling and fixing material of the present invention, the specific process is:

Step 1, prepare a hydrogen peroxide solution with a volume fraction of 20%, and dissolve the drug in the hydrogen peroxide solution at a mass volume percentage of 5 mg/ml at the same time, referred to as solution A; prepare a polyethylene glycol (PEG) solution with a concentration of 75 mg/ml, referred to as solution B; prepare a catalase solution with a concentration of 100 μg/ml, referred to as solution C; the drug is vancomycin hydrochloride; PEG is PEG2000;

Step 2: First, according to 20% of the solution volume (mg/ml), ultrasonically disperse a certain mass of montmorillonite evenly in solution A, then stir for 1 hour to carry out hydrogen peroxide and drug loading, after the stirring is completed, suction filter and vacuum dry ; Then redisperse the once-dried montmorillonite in solution B for PEG coating and stirring, the stirring time is 1h, suction filter after the stirring, and vacuum-dry; finally, re-disperse the second-dried montmorillonite in the solution Carry out the carrying of catalase in C, the stirring time is 1h, suction filtration after stirring, and vacuum drying; repeat this step 3 times, utilize the layer-by-layer self-assembly technology to obtain the hydrogen peroxide/antibiotic zero-order release carrier;

Step 3, mix the PMMA powder, the initiator BPO and the hydrogen peroxide/antibiotic zero-order release carrier obtained in step 2 evenly according to the mass ratio of 49.95:0.05:50 to obtain the filling and fixing material component D;

Step 4: Mix MMA, N,N-dimethyl-p-toluidine, and hydroquinone evenly in a mass ratio of 96.5:3:0.5 to obtain the filling and fixing material component E;

Step 5, mix the filling and fixing material component D obtained in step 3 with the filling and fixing material component E obtained in step 4 according to a solid-to-liquid ratio of 1 g/ml, stir, and fill the open wound site by injection, and the fixing material to be filled After component D reacts with component E of the filling and fixing material, the wound site can be further fixed.

Example 2

The concrete process of the preparation method of self-oxygen supply antibacterial filling and fixing material of the present invention is:

Step 1, prepare a hydrogen peroxide solution with a volume fraction of 40%, and dissolve the drug in the hydrogen peroxide solution at a mass volume percentage of 15 mg/ml at the same time, referred to as solution A; prepare a polyethylene glycol (PEG) solution with a concentration of 115 mg/ml, referred to as solution B; prepare the catalase solution of concentration 400 μ g/ml, be called for short solution C; Medicine selects levofloxacin hydrochloride for use; PEG is PEG4000;

Step 2, according to 40% (mg/ml) of the volume of the solution, ultrasonically disperse a certain mass of montmorillonite evenly in the solution A, then stir for 2 hours to carry out hydrogen peroxide and drug loading, after the stirring is completed, suction filter and vacuum dry; Then redisperse the once-dried montmorillonite in solution B for PEG-coating and stirring, the stirring time is 2h, suction filter after the stirring, and vacuum-dry; finally, re-disperse the second-dried montmorillonite in solution C Carry out the carrying of catalase in the medium, the stirring time is 2h, suction filtration after stirring, and vacuum drying; repeat this step 8 times, utilize the layer-by-layer self-assembly technology to obtain the hydrogen peroxide/antibiotic zero-order release carrier;

Step 3, mix the PMMA powder, the initiator BPO and the hydrogen peroxide/antibiotic zero-order release carrier obtained in step 2 evenly according to the mass ratio of 69.85:0.15:30 to obtain the filling and fixing material component D;

Step 4: Mix MMA, N,N-dimethyl-p-toluidine, and hydroquinone evenly in a mass ratio of 99.5:0.4:0.1 to obtain the filling and fixing material component E;

Step 5, mix the filling and fixing material component D obtained in step 3 with the filling and fixing material component E obtained in step 4 according to the solid-liquid ratio of 2 g/ml, stir, and fill the open fracture site by injection, and the fixing material to be filled After component D reacts with component E of the filling and fixing material, the wound site can be further fixed.

Example 3

The concrete process of the preparation method of self-oxygen supply antibacterial filling and fixing material of the present invention is:

Step 1, prepare a hydrogen peroxide solution with a volume fraction of 25%, and dissolve the drug in the hydrogen peroxide solution at a mass volume percentage of 7.5 mg/ml at the same time, referred to as solution A; prepare a polyethylene glycol (PEG) solution with a concentration of 85 mg/ml, referred to as Solution B; prepare a catalase solution with a concentration of 200 μg/ml, referred to as solution C; the drug is tetracycline hydrochloride, and the PEG is PEG6000;

Step 2, according to 25% (mg/ml) of the volume of the solution, ultrasonically disperse a certain mass of montmorillonite in the solution A, and then stir for 2 hours to carry out hydrogen peroxide and drug loading, after the stirring is completed, suction filtration, vacuum drying, Then redisperse the once-dried montmorillonite in solution B for PEG coating and stirring, the stirring time is 2h, suction filter after the stirring, and vacuum-dry, and finally re-disperse the second-dried montmorillonite in solution C Carry out the carrying of catalase in the medium, the stirring time is 2h, suction filtration after stirring, and vacuum drying; repeat this step 4 times, utilize layer-by-layer self-assembly technology to obtain carrying hydrogen peroxide/antibiotics zero-order release carrier;

Step 3, mix the PMMA powder, the initiator BPO and the hydrogen peroxide/antibiotic zero-order release carrier obtained in step 2 evenly according to the mass ratio of 64.85:0.15:35 to obtain the filling and fixing material component D;

Step 4: Mix MMA, N,N-dimethyl-p-toluidine, and hydroquinone evenly in a mass ratio of 98.5:1:0.5 to obtain the filling and fixing material component E;

Step 5, mix the filling and fixing material component D obtained in step 3 with the filling and fixing material component E obtained in step 4 according to a solid-to-liquid ratio of 1.5 g/ml, stir, and fill the open fracture site by injection, to be filled and fixed After the reaction between the material component D and the filling and fixing material component E is completed, the wound site can be further fixed.

Example 4

The concrete process of the preparation method of self-oxygen supply antibacterial filling and fixing material of the present invention is:

Step 1, prepare a hydrogen peroxide solution with a volume fraction of 30%, and dissolve the drug in the hydrogen peroxide solution at a mass volume percentage of 10 mg/ml at the same time, referred to as solution A; prepare a polyethylene glycol (PEG) solution with a concentration of 115 mg/ml, referred to as solution B; prepare a catalase solution with a concentration of 300 μg/ml, referred to as solution C; the drug is ceftriaxone sodium, and the PEG is PEG8000;

Step 2: According to 30% (mg/ml) of the solution volume, ultrasonically disperse a certain mass of montmorillonite in solution A, then stir for 1.5 hours to carry out hydrogen peroxide and drug loading, after the stirring is completed, suction filter and vacuum dry ; Then redisperse the once-dried montmorillonite in solution B for PEG coating and stirring, the stirring time is 2h, suction filter after stirring, and dry in vacuum; finally re-disperse the second-dried montmorillonite in the solution Carry out the carrying of catalase in C, the stirring time is 1.5h, suction filtration after stirring, and vacuum drying; repeat this step 5 times, use the layer-by-layer self-assembly technology to obtain the hydrogen peroxide/antibiotic zero-order release carrier;

Step 3, mix the PMMA powder, the initiator BPO and the hydrogen peroxide/antibiotic zero-order release carrier obtained in step 2 evenly according to the mass ratio of 62.95:0.05:37 to obtain the filling and fixing material component D;

Step 4: Mix MMA, N,N-dimethyl-p-toluidine, and hydroquinone evenly in a mass ratio of 97.5:2.1:0.4 to obtain the filling and fixing material component E;

Step 5, mix the filling and fixing material component D obtained in step 3 with the filling and fixing material component E obtained in step 4 according to the solid-liquid ratio of 2 g/ml, stir, and fill the open fracture site by injection, and the fixing material to be filled After component D reacts with component E of the filling and fixing material, the wound site can be further fixed.

Example 5

The concrete process of the preparation method of self-oxygen supply antibacterial filling and fixing material of the present invention is:

Step 1, prepare a hydrogen peroxide solution with a volume fraction of 35%, and dissolve the drug in the hydrogen peroxide solution at a mass volume percentage of 15 mg/ml at the same time, referred to as solution A, and prepare a polyethylene glycol (PEG) solution with a concentration of 115 mg/ml, referred to as solution B; prepare a catalase solution with a concentration of 350 μg/ml, referred to as solution C; the drug is vancomycin hydrochloride, and the PEG is PEG10000;

Step 2, according to 40% (mg/ml) of the volume of the solution, ultrasonically disperse a certain mass of montmorillonite evenly in the solution A, then stir for 2 hours to carry out hydrogen peroxide and drug loading, after the stirring is completed, suction filter and vacuum dry; Then redisperse the once-dried montmorillonite in solution B for PEG-coating and stirring, the stirring time is 2h, suction filter after the stirring, and vacuum-dry; finally, re-disperse the second-dried montmorillonite in solution C Carry out the carrying of catalase, the stirring time is 1h, suction filtration after stirring, and vacuum drying; repeat this step 7 times, use the layer-by-layer self-assembly technology to obtain the hydrogen peroxide/antibiotic zero-order release carrier;

Step 3, mix the PMMA powder, the initiator BPO and the hydrogen peroxide/antibiotic zero-order release carrier obtained in step 2 evenly according to the mass ratio of 59.85:0.15:40 to obtain the filling and fixing material component D;

Step 4: Mix MMA, N,N-dimethyl-p-toluidine, and hydroquinone evenly in a mass ratio of 96.5-99.5:0.3-3:0.1-0.5 to obtain the filling and fixing material component E;

Step 5, mix the filling and fixing material component D obtained in step 3 with the filling and fixing material component E obtained in step 4 according to the solid-liquid ratio of 1g/ml, stir, and fill the open fracture site by injection, and the fixing material to be filled After component D reacts with component E of the filling and fixing material, the wound site can be further fixed.

Experiments have proved that the oxygen release and antibiotic release of the self-oxygenated antibacterial filling and fixing material obtained by the preparation method of the present invention follow zero-order release kinetics in a simulated human environment, avoiding side effects caused by sudden release of drugs and oxygen. After the experimental use, the immobilization, infection prevention and antibacterial treatment of the material all performed well.

Claims (6)

1. A preparation method for self-oxygenation antibacterial filling and fixing material is characterized in that, it is implemented according to the following steps: Step 1, preparing a hydrogen peroxide solution, dissolving the drug in the hydrogen peroxide solution according to the mass volume percentage of 5-15 mg/ml, referred to as solution A; preparing a polyethylene glycol solution, referred to as solution B; preparing a catalase solution, referred to as solution C; Step 2: Add montmorillonite to solution A, after ultrasonic dispersion, carry out stirring with hydrogen peroxide and drug load, suction filter after stirring, and dry in vacuum; then redisperse the once-dried montmorillonite in solution B , carry out PEG coating and stirring, suction filtration after the stirring, and vacuum drying; finally, redisperse the secondary dried montmorillonite in solution C, carry out stirring with catalase, suction filtration after the stirring, and vacuum drying ; Repeat this step multiple times to obtain a zero-order release carrier loaded with hydrogen peroxide/antibiotics; Step 3, mix the PMMA powder, the initiator BPO, and the hydrogen peroxide/antibiotic zero-order release carrier obtained in step 2 to obtain the filling and fixing material component D; Step 4, uniformly mixing MMA, N,N-dimethyl-p-toluidine, and hydroquinone to obtain the filling and fixing material component E; Step 5, mix the filling and fixing material component D obtained in step 3 with the filling and fixing material component E obtained in step 4, stir, and fill the open fracture site by injection, the filling and fixing material component D and the filling and fixing material component E After the reaction is over, it's done. 2. the preparation method of self-oxygenated antibacterial filling and fixing material according to claim 1, is characterized in that, the volume fraction of described hydrogen peroxide solution is 20～40%, the concentration of polyethylene glycol solution is 75～115mg/ ml, the concentration of catalase solution is 100-400 μg/ml; The drug is selected from one of vancomycin hydrochloride, levofloxacin hydrochloride, tetracycline hydrochloride or ceftriaxone sodium; The PEG is selected from one of PEG2000, PEG4000, PEG6000, PEG8000 or PEG10000. 3. the preparation method of self-oxygenated antibacterial filling and fixing material according to claim 1, is characterized in that, the quality of described montmorillonite is 20～40% of solution A volume, and hydrogen peroxide and medicine load stir. The time is 1-2 hours, the time for PEG-coating and stirring is 1-2 hours, the time for carrying catalase and stirring is 1-2 hours, and the number of repeated cycles is 3-8 times. 4. The preparation method of self-oxygenated antibacterial filling and fixing material according to claim 1, characterized in that, the mass ratio of the PMMA powder, initiator BPO and hydrogen peroxide/antibiotic zero-order release carrier is 49.95～69.85 : 0.05~0.15: 30~50. 5. the preparation method of self-oxygenation antibacterial filling and fixing material according to claim 1 is characterized in that, the mass ratio of described MMA, N, N-dimethyl-p-toluidine, hydroquinone three is 96.5-99.5: 0.3-3: 0.1-0.5. 6. The method for preparing self-oxygenated antibacterial filling and fixing material according to claim 1, characterized in that, the filling and fixing material component D and the filling and fixing material component E are mixed according to a solid-liquid ratio of 1 to 2 g/ml.