CN116026906A - Microfluidic blood sugar detection chip - Google Patents
Microfluidic blood sugar detection chip Download PDFInfo
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- CN116026906A CN116026906A CN202211705136.3A CN202211705136A CN116026906A CN 116026906 A CN116026906 A CN 116026906A CN 202211705136 A CN202211705136 A CN 202211705136A CN 116026906 A CN116026906 A CN 116026906A
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- blood
- electrode
- detection chip
- negative pressure
- microfluidic
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- 239000008280 blood Substances 0.000 title claims abstract description 94
- 210000004369 blood Anatomy 0.000 title claims abstract description 94
- 238000001514 detection method Methods 0.000 title claims abstract description 36
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 31
- 239000000463 material Substances 0.000 claims abstract description 25
- 238000001179 sorption measurement Methods 0.000 claims abstract description 22
- 108090000790 Enzymes Proteins 0.000 claims abstract description 18
- 102000004190 Enzymes Human genes 0.000 claims abstract description 18
- 239000011248 coating agent Substances 0.000 claims abstract description 15
- 238000000576 coating method Methods 0.000 claims abstract description 15
- 239000004033 plastic Substances 0.000 claims abstract description 12
- 229920003023 plastic Polymers 0.000 claims abstract description 12
- 239000000758 substrate Substances 0.000 claims abstract description 10
- 238000002347 injection Methods 0.000 claims abstract description 7
- 239000007924 injection Substances 0.000 claims abstract description 7
- 238000007789 sealing Methods 0.000 claims abstract description 4
- 229940088598 enzyme Drugs 0.000 claims description 16
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 claims description 11
- 239000008103 glucose Substances 0.000 claims description 11
- 239000011358 absorbing material Substances 0.000 claims description 5
- 230000017531 blood circulation Effects 0.000 claims description 4
- 108010050375 Glucose 1-Dehydrogenase Proteins 0.000 claims description 3
- 108010015776 Glucose oxidase Proteins 0.000 claims description 3
- 239000004366 Glucose oxidase Substances 0.000 claims description 3
- 229940116332 glucose oxidase Drugs 0.000 claims description 3
- 235000019420 glucose oxidase Nutrition 0.000 claims description 3
- 239000007788 liquid Substances 0.000 claims description 3
- 238000010241 blood sampling Methods 0.000 abstract description 8
- 238000010521 absorption reaction Methods 0.000 abstract description 6
- 238000005457 optimization Methods 0.000 description 7
- WQZGKKKJIJFFOK-VFUOTHLCSA-N beta-D-glucose Chemical compound OC[C@H]1O[C@@H](O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-VFUOTHLCSA-N 0.000 description 5
- 239000000203 mixture Substances 0.000 description 5
- 230000000694 effects Effects 0.000 description 4
- 238000010586 diagram Methods 0.000 description 3
- 239000000243 solution Substances 0.000 description 3
- 238000005842 biochemical reaction Methods 0.000 description 2
- 238000000034 method Methods 0.000 description 2
- 208000012260 Accidental injury Diseases 0.000 description 1
- 208000017667 Chronic Disease Diseases 0.000 description 1
- RYGMFSIKBFXOCR-UHFFFAOYSA-N Copper Chemical compound [Cu] RYGMFSIKBFXOCR-UHFFFAOYSA-N 0.000 description 1
- 229920000742 Cotton Polymers 0.000 description 1
- 229910000831 Steel Inorganic materials 0.000 description 1
- 230000002411 adverse Effects 0.000 description 1
- 238000004458 analytical method Methods 0.000 description 1
- 230000000740 bleeding effect Effects 0.000 description 1
- 238000004364 calculation method Methods 0.000 description 1
- 239000003153 chemical reaction reagent Substances 0.000 description 1
- 229910052802 copper Inorganic materials 0.000 description 1
- 239000010949 copper Substances 0.000 description 1
- 206010012601 diabetes mellitus Diseases 0.000 description 1
- 208000002173 dizziness Diseases 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 150000002191 fatty alcohols Chemical class 0.000 description 1
- 208000015181 infectious disease Diseases 0.000 description 1
- 208000014674 injury Diseases 0.000 description 1
- 239000000976 ink Substances 0.000 description 1
- 239000012528 membrane Substances 0.000 description 1
- 239000007769 metal material Substances 0.000 description 1
- 229940051841 polyoxyethylene ether Drugs 0.000 description 1
- 229920000056 polyoxyethylene ether Polymers 0.000 description 1
- 229910052709 silver Inorganic materials 0.000 description 1
- 239000004332 silver Substances 0.000 description 1
- 239000010959 steel Substances 0.000 description 1
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- Measurement Of The Respiration, Hearing Ability, Form, And Blood Characteristics Of Living Organisms (AREA)
Abstract
The invention discloses a microfluidic blood sugar detection chip, which comprises a blood collection unit, a blood detection unit and a plastic shell, and is characterized in that: the blood collection unit comprises a negative pressure interface, an annular collecting groove, a diversion trench and a collection assembly, wherein the collection assembly comprises an injection port, pure water and a rubber plug, the pure water is sealed in a cavity formed by the rubber plug and a plastic shell, the outer side part of the rubber plug is connected with a motor ejector rod of a matched instrument, and the injection port is sealed by a sealing film; the blood detection unit comprises a blood guide material, an enzyme coating, an insulating layer, a substrate, an electrode, an adsorption groove, a flow channel and an electrode interface, wherein a water absorption material is arranged in the adsorption groove, and the flow channel is arranged above the adsorption groove; an electrode, an insulating layer, an enzyme coating and a blood guide material are arranged above the substrate in a layered and overlapped mode from bottom to top; the electrode interface is connected with an electrode thimble of a matched instrument; the blood guiding material is connected with the negative pressure interface. The finger of the user is put on the detection chip, so that the automatic needleless blood sampling, sample loading and blood sugar detection functions can be realized, the operation is very simple, and the degree of automation is high; when the collection assembly adopts needleless design, the pain is little, reduces fear of the terrorist needle patient.
Description
Technical Field
The invention relates to the technical field of blood sugar detection equipment, in particular to a microfluidic blood sugar detection chip.
Background
Diabetes is a common chronic disease, and patients need to monitor and detect blood sugar frequently, so that too high and too low blood sugar can have adverse effects on the body and even have life hazards. The current common blood sugar detection mode mainly comprises the steps of using a blood sampling pen and a blood sampling needle to puncture finger fingertip skin, then extruding blood to drop on blood sugar detection test paper, inserting the test paper into a blood sugar detector, and finally reading out data and displaying the data by the blood sugar detector. The way has three problems, namely, the pain feeling of the steel needle blood sampling is obvious, the pain exists in each blood sampling of a patient, and the psychological fear feeling is very strong; secondly, the needle of the blood sampling pen is very sharp, so that other parts of the body are very easy to puncture, and accidental injury or infection is caused; thirdly, the manual operation steps are complex, a patient or medical staff is required to manually put the blood taking needle on the blood taking pen, the finger skin is pricked, blood is dripped on the test paper strip, and then the test paper strip is inserted into the detector for detection, so that the use of the test paper strip matched with the conventional mode is inconvenient for the patient, especially the old, and the test paper strip matched with the conventional mode has only a detection function and has no functions of blood collection and interference removal.
The conventional blood glucose test paper is shown in FIG. 3, and the structural components of the conventional blood glucose test paper comprise a 1 'substrate, a 2' electrode, a 3 'insulating layer, a 4' enzyme coating, a 5 'blood guiding material and a 6' cover plate. The using process comprises the following steps: firstly, a blood taking needle is required to be put into a blood taking pen, then the skin of the finger tip is pricked by the blood taking pen, blood is squeezed out, a test strip is taken up, a bleeding finger is close to a 5 blood guiding material of the test strip, the blood guiding material adsorbs blood and rapidly diffuses onto a 4 enzyme coating, at the moment, a user inserts the test strip into a blood sugar detecting instrument, the instrument detects a current signal which appears on the test strip due to biochemical reaction, and then calculation and result display are carried out. The disadvantage of this solution is:
1. the reagent strip related to the scheme needs to be manually used for blood collection by a blood collection pen and a blood collection needle, and the steps are complicated;
2. this solution requires manual access of blood to the test strip and the amount of blood drawn in is not well controlled.
3. According to the scheme, the blood taking needle can be used for directly seeing the needle head, so that psychological fear of a patient is increased;
4. the proposal can directly see red blood and has negative effect on the mind of the patient with the dizziness.
Disclosure of Invention
The invention aims to provide a microfluidic blood sugar detection chip for solving the problems in the background technology.
In order to achieve the above purpose, the present invention provides the following technical solutions: the microfluidic blood sugar detection chip comprises a blood collection unit, a blood detection unit and a plastic shell, wherein the blood collection unit comprises a negative pressure interface, an annular collecting groove, a diversion trench and a collection assembly, the collection assembly comprises a jet orifice, pure water and a rubber plug, the pure water is sealed in a cavity formed by the rubber plug and the plastic shell, the outer side part of the rubber plug is connected with a motor ejector rod of a matched instrument, and the jet orifice is sealed by a sealing film;
the blood detection unit comprises a blood guide material, an enzyme coating, an insulating layer, a substrate, an electrode, an adsorption groove, a flow channel and an electrode interface, wherein a water absorption material is arranged in the adsorption groove, and the flow channel is arranged above the adsorption groove;
an electrode, an insulating layer, an enzyme coating and a blood guide material are arranged above the substrate in a layered and overlapped mode from bottom to top;
the electrode interface is connected with an electrode thimble of a matched instrument;
the blood guiding material is connected with the negative pressure interface.
As a further optimization, the pure water is connected with the jet port through a flow passage.
As a further optimization, the annular collecting groove is arranged on the inner side of the diversion groove, so that the blood flow is prevented from being blocked after being pressed by the skin.
As further optimization, the adsorption groove is arranged below the flow channel and is used for removing the mixture of pure water and blood after puncturing the skin, so that the blood glucose concentration is prevented from being diluted, and the detection result is prevented from being influenced.
As a further optimization, the adsorption tank is internally provided with a water absorbing material.
As a further optimization, the enzyme type used for the enzyme coating is glucose oxidase or glucose dehydrogenase.
As a further optimization, the outer side of the plastic shell is provided with a negative pressure interface which is used for negative pressure drainage, so that blood flows out from the puncture on the finger and enters the diversion trench.
As a further optimization, the negative pressure interface is provided with a filter element which is breathable but not permeable to liquid, so that excessive blood is prevented from entering the negative pressure system.
Advantageous effects
According to the microfluidic blood sugar detection chip provided by the invention, the functions of automatic needleless blood sampling, sample loading and blood sugar detection can be realized by putting the fingers of a user on the detection chip, the operation is very simple, and the degree of automation is high; when the collection assembly is in a needleless design, pain is small, and fear of a needle-terrible patient is reduced; considering the influence of pure water on the blood concentration when the pure water punctures the skin, the mixture of the pure water and the blood is absorbed by adopting the adsorption groove and the adsorption material, so that the detection accuracy is effectively improved.
Drawings
FIG. 1 is a schematic diagram of the overall structure position relationship of the present invention;
FIG. 2 is a schematic diagram of an acquisition assembly according to an embodiment of the present invention;
fig. 3 is a schematic structural diagram of a conventional apparatus in the background of the invention.
Reference numerals
The blood guide material 1, the negative pressure interface 2, the enzyme coating 3, the insulating layer 4, the substrate 5, the electrode 6, the adsorption groove 7, the runner 8, the annular collecting groove 9, the diversion groove 10, the jet orifice 11, the pure water 12, the rubber plug 13, the electrode interface 14 and the plastic shell 15.
Detailed Description
The following description of the embodiments of the present invention will be made clearly and completely with reference to the accompanying drawings, in which it is apparent that the embodiments described are only some embodiments of the present invention, but not all embodiments. All other embodiments, which can be made by those skilled in the art based on the embodiments of the invention without making any inventive effort, are intended to be within the scope of the invention.
Referring to fig. 1 and 2, a microfluidic blood glucose detection chip comprises a blood collection unit, a blood detection unit and a plastic shell 15, wherein the blood collection unit comprises a negative pressure interface 2, an annular collecting groove 9, a diversion trench 10 and a collection assembly, the collection assembly comprises an injection port 11, pure water 12 and a rubber plug 13, the pure water 12 is sealed in a cavity formed by the rubber plug 13 and the plastic shell 15, the outer side part of the rubber plug 13 is connected with a motor ejector rod of a matched instrument, and the injection port 11 is sealed by a sealing film.
The blood detection unit comprises a blood guide material 1, an enzyme coating 3, an insulating layer 4, a substrate 5, an electrode 6, an adsorption groove 7, a runner 8 and an electrode interface 14, wherein a water absorption material is arranged in the adsorption groove 7, and the water absorption material comprises but is not limited to a hydrophilic material and water absorption cotton; the runner 8 is arranged above the adsorption groove 7; an electrode 6, an insulating layer 4, an enzyme coating 3 and a blood guide material 1 are arranged above the substrate 5 in a layered and overlapped mode from bottom to top, the electrode 6 comprises metal materials such as conductive ink, copper, silver and the like, enzyme types adopted by the enzyme coating 3 comprise but are not limited to glucose oxidase, glucose dehydrogenase and the like, and an electrode interface 14 is connected with an electrode thimble of a matched instrument; the blood guiding material 1 is connected with the negative pressure interface 2, the blood guiding material 1 is a hydrophilic membrane and comprises but is not limited to fatty alcohol polyoxyethylene ether material, pure water 12 is connected with the jet orifice 11 through the runner 8, the inner side of the annular collecting groove 9 is provided with a diversion groove 10 for preventing blood flow from being blocked after being compressed by skin, the lower part of the runner 8 is provided with an adsorption groove 7 for removing a mixture of pure water and blood after puncturing the skin, the concentration of blood sugar is prevented from being diluted, the detection result is prevented from being influenced, and the absorption capacity of the water absorbing material in the adsorption groove is set to only absorb 2-3 drops of the mixture of pure water and blood.
In the present embodiment, when pure water 12 is injected from the injection port 11 at a high speed and pierces the skin under the pressurizing action of an external instrument, thereby realizing the function of needleless blood collection.
In this embodiment, the plastic housing 15 has a negative pressure port 2 on the outside for negative pressure drainage to allow blood to flow out of the finger puncture and into the channel 10.
In this embodiment, the negative pressure port 2 is provided with a gas-permeable, liquid-impermeable filter element, which prevents excessive blood from entering the negative pressure system.
Blood sampling principle: when the device is used, a user puts the detection chip into a matched instrument, then places the sterilized finger on a collecting assembly consisting of the annular collecting groove 9, the diversion trench 10 and the jet opening 11, and controls a spring in the instrument to quickly strike the piston through a button on the instrument, so that pure water 12 is sprayed onto the finger through the jet opening 11 at a high speed, the skin is punctured by high-speed liquid flow, and the flowing blood enters the annular collecting groove 9 and the flow channel 8 through the diversion trench 10 under the negative pressure effect of the negative pressure interface 2.
Detection principle: the adsorption groove 7 is arranged below the flow channel 8, so that a part of the blood mixture with pure water which firstly enters the flow channel can be adsorbed, when a certain amount of adsorbed water absorbing material is not absorbed, blood directly flows into the blood guiding material 1 from the flow channel 8, rapidly diffuses in the blood, and enters the enzyme coating 3 downwards to carry out biochemical reaction, and the generated weak current is captured by the electrode 6 and sent into the instrument for analysis and display through the electrode interface 14.
The principles and embodiments of the present invention have been described in detail with reference to specific examples, which are provided to facilitate understanding of the method and core ideas of the present invention; meanwhile, as those skilled in the art will have variations in the specific embodiments and application scope in accordance with the ideas of the present invention, the present description should not be construed as limiting the present invention in view of the above.
Claims (8)
1. The utility model provides a micro-fluidic blood sugar detects chip, includes blood collection unit, blood detecting element and plastics casing (15), its characterized in that: the blood collection unit comprises a negative pressure interface (2), an annular collecting groove (9), a diversion trench (10) and a collection assembly, wherein the collection assembly comprises an injection port (11), pure water (12) and a rubber plug (13), the pure water (12) is sealed in a cavity formed by the rubber plug (13) and a plastic shell (15), the outer side part of the rubber plug (13) is connected with a motor ejector rod of a matched instrument, and the injection port (11) is sealed by a sealing film;
the blood detection unit comprises a blood guide material (1), an enzyme coating (3), an insulating layer (4), a substrate (5), an electrode (6), an adsorption groove (7), a runner (8) and an electrode interface (14), wherein a water absorbing material is arranged in the adsorption groove (7), and the runner (8) is arranged above the adsorption groove (7);
an electrode (6), an insulating layer (4), an enzyme coating (3) and a blood guiding material (1) are arranged above the substrate (5) in a layered and overlapped mode from bottom to top;
the electrode interface (14) is connected with an electrode thimble of a matched instrument;
the blood guiding material (1) is connected with the negative pressure interface (2).
2. The microfluidic blood glucose detection chip according to claim 1, wherein: the pure water (12) is connected with the jet orifice (11) through the flow passage (8).
3. The microfluidic blood glucose detection chip according to claim 1, wherein: the annular collecting groove (9) is arranged on the inner side of the diversion trench (10).
4. The microfluidic blood glucose detection chip according to claim 1, wherein: the adsorption groove (7) is arranged below the flow channel (8).
5. The microfluidic blood glucose detection chip according to claim 4, wherein: the adsorption groove (7) is internally provided with a water absorbing material.
6. The microfluidic blood glucose detection chip according to claim 1, wherein: the enzyme type adopted by the enzyme coating (3) is glucose oxidase or glucose dehydrogenase.
7. The microfluidic blood glucose detection chip according to claim 4, wherein: the outside of the plastic shell (15) is provided with a negative pressure interface (2) which is used for negative pressure drainage, so that blood flows out from a puncture on a finger and enters the diversion trench (10).
8. The microfluidic blood glucose detection chip according to claim 4, wherein: the negative pressure interface (2) is provided with a filter element which is breathable but not permeable to liquid.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202211705136.3A CN116026906A (en) | 2022-12-29 | 2022-12-29 | Microfluidic blood sugar detection chip |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202211705136.3A CN116026906A (en) | 2022-12-29 | 2022-12-29 | Microfluidic blood sugar detection chip |
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| Publication Number | Publication Date |
|---|---|
| CN116026906A true CN116026906A (en) | 2023-04-28 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202211705136.3A Pending CN116026906A (en) | 2022-12-29 | 2022-12-29 | Microfluidic blood sugar detection chip |
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Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1566939A (en) * | 2003-06-18 | 2005-01-19 | 清华大学 | Biomedical anodynia blood taking micro needle chip and method for making same |
| WO2005120365A1 (en) * | 2004-06-03 | 2005-12-22 | Pelikan Technologies, Inc. | Method and apparatus for a fluid sampling device |
| WO2007108519A1 (en) * | 2006-03-22 | 2007-09-27 | Matsushita Electric Industrial Co., Ltd. | Blood test apparatus |
| CN101404935A (en) * | 2006-03-22 | 2009-04-08 | 松下电器产业株式会社 | Blood test device |
| CN104380095A (en) * | 2012-06-01 | 2015-02-25 | 爱-森斯株式会社 | Electrochemical biosensor with improved accuracy |
-
2022
- 2022-12-29 CN CN202211705136.3A patent/CN116026906A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1566939A (en) * | 2003-06-18 | 2005-01-19 | 清华大学 | Biomedical anodynia blood taking micro needle chip and method for making same |
| WO2005120365A1 (en) * | 2004-06-03 | 2005-12-22 | Pelikan Technologies, Inc. | Method and apparatus for a fluid sampling device |
| WO2007108519A1 (en) * | 2006-03-22 | 2007-09-27 | Matsushita Electric Industrial Co., Ltd. | Blood test apparatus |
| CN101404935A (en) * | 2006-03-22 | 2009-04-08 | 松下电器产业株式会社 | Blood test device |
| CN104380095A (en) * | 2012-06-01 | 2015-02-25 | 爱-森斯株式会社 | Electrochemical biosensor with improved accuracy |
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