CN115819153A - Rose dreg bio-organic fertilizer and preparation method and application thereof - Google Patents

Abstract

The invention relates to a rose residue bio-organic fertilizer and a preparation method and application thereof, belonging to the technical field of microbial fertilizers. Rose dregs are rich in sugar, amino acids and other nutrients. EM bacteria and salt-preserved Halobacillus salinus (Halobacillus salinus) CGMCC NO.1.8606 are used as fermentation bacteria, and rose dregs are used as fermentation substrates to ferment to produce bio-organic Fertilizer can not only improve the utilization rate of rose slag, but also the prepared bio-organic fertilizer can replace inorganic fertilizer, reduce the amount of inorganic fertilizer used in crop production, and alleviate various problems caused by excessive use of inorganic fertilizer; the prepared bio-organic fertilizer Organic fertilizers can effectively increase crop yield, promote early maturity of crops, significantly reduce the delivery time of crop maturity, prolong the harvest period of crops, improve crop quality, and produce a variety of substances required by plants.

Description

A kind of rose residue bio-organic fertilizer and its preparation method and application

technical field

The invention relates to a rose residue bio-organic fertilizer and its preparation method and application, and belongs to the technical field of marine microorganism application and microbial fertilizer.

Background technique

Rose (Rosa rugosa) is a perennial evergreen or deciduous shrub of Rosaceae Rosa, which is widely planted in the world. my country is one of the big rose planting countries, and has abundant rose resources, with as many as 95 species in more than 200 Rosa genus. The main varieties of roses planted in China are Fenghua Rose and Kushui Rose, which are mainly distributed in Pingyin, Shandong, Yongdeng, Gansu and other places, with a total cultivation area of about 7000hm ² . Roses contain essential oils and pigments, and the essential oils and pigments extracted from roses have many advantages. Rose essential oil has an elegant, soft, delicate, sweet and honey-like rose fragrance, and is known as "liquid gold". The extraction of rose essential oil generally adopts the process of steam distillation, and the extraction rate of essential oil is about 0.25-0.3‰, which will produce a large amount of rose residue. Rose pigment is easily soluble in water and is an excellent natural pigment. Its use in food can effectively make up for the shortcomings of chemically synthesized pigments. After the rose pigment is extracted, a large amount of rose residue will also be produced. In addition, rose is a natural raw material with rich nutrients, which contains a variety of trace elements, dietary fiber and essential amino acids and other nutrients. In recent years, roses have also been used in the brewing process to extract the active ingredients in roses, or directly add rose flowers or dried flowers to the beer brewing process to endow beer with the flavor or efficacy of roses. This process also produces a large amount of rose pomace.

In actual production, rose dregs are mostly thrown away or burned as waste, which not only pollutes the environment, but is also a waste of rose dregs resources. Weiqin and others analyzed the nutritional components contained in rose dregs, and the results showed that its sugar content was 29.5%, of which soluble sugar was 8.8%; the total amount of amino acids was 10.205%, including 8 kinds of essential amino acids for human body; it also contains VC , VE, β-carotene and Ca, Fe, M n and other mineral elements. (Weiqin et al. Research on the chemical and nutritional components of rose dregs[J]. Journal of Northwest Forestry University, 2005, 20(3): 140-141.) Fan Zhujun and others studied the rose dregs after extracting essential oils by steam distillation The components were analyzed, and the glucose content was 18.33%, the starch content was 21.75%, the crude fat content was 3.09%, the crude protein content was 12.40%, and the crude fiber content was 21.28%. (Fan Zhujun et al. Preliminary research on the composition of rose dregs [J]. Natural product research and development, 1990 (1): 67-69.) Xie Qiutao determined that the total relative content of dietary fiber in rose dregs after oil extraction and pigment extraction was 35.9%. , wherein the relative content of non-water-soluble fiber is 32.9%, and the relative content of water-soluble fiber is 3.0%. (Xie Qiutao. Supercritical CO ₂ extraction process optimization of rose essential oil and comprehensive utilization of by-products [D]. Changsha: Central South University, 2013.) From the analysis results of trace elements, nutritional components, amino acid content of rose residue, it can be seen that, Rose pomace is a natural raw material with rich nutrients, which is worthy of further development and utilization.

Chinese patent document CN104351839A (application number 201410618093.4) provides a method for preparing salted eggs from rose dregs, comprising the following steps: heating and boiling rose dregs, controlling the salt content to 15% to 18% to obtain rose dregs liquid; Cool the rose dregs solution, then add sodium dehydroacetate and potassium sorbate, and mix well to obtain a rose dregs pickling solution; immerse fresh eggs in the rose dregs pickling solution, seal it, and store it at 20°C Marinate for 25 to 30 days at ~28°C to complete the preparation of the salted eggs. The salted egg prepared by the rose dregs in the invention has the advantages of simple preparation, unique flavor of the salted egg, good taste, high nutritional value and the like.

Chinese patent document CN106420537A (application number 201611059183.X) discloses a rose dregs exfoliating massage bath cream, which is mainly composed of rose dregs, highland barley powder, oat powder, rose petals, rose essential oil, Olive oil, petroleum jelly, non-ionic surfactant composition. Most of the exfoliating products currently available on the market are aimed at facial skin, which contain chemical substances, which are more irritating to the skin, and long-term use will cause skin problems. The rose dregs exfoliating massage bath cream of the present invention is composed of pure natural plant components, and the rose dregs are used as the main component of the exfoliating massage bath cream to avoid waste of resources. It will not produce irritation and side effects during use, can be used for a long time, and is suitable for whole body exfoliation. The rose dregs exfoliating massage bath cream can gently remove the body's keratin accumulation caused by metabolism, dryness and lack of water, soothe the skin, effectively repair dry skin, and keep the skin moisturized for a long time.

Chinese patent document CN108277113A (application number 201810252210.8) discloses a rose residue soap, which is mainly composed of edible rose residue, edible rose petals, olive oil, coconut oil, palm oil, sodium hydroxide, distilled water , citric acid, edible ethanol, sodium chloride, sodium chloride composition. The rose dregs soap of the present invention does not contain harmful substances to the human body, does not produce side effects on the human body during use, and can be used for a long time; and the edible rose dregs are used as the main component of the soap, which improves the health of the rose dregs. Utilization, to avoid waste of resources and environmental pollution. Rose dregs soap has an elegant and long-lasting, pleasant rose fragrance, which can sterilize and antibacterial, clean the skin, prevent skin aging, make people feel comfortable and happy, relieve anxiety and depression, adjust female endocrine and menstrual cycle, and also have the effect of removing athlete's foot and Good effect on foot odor.

In addition, the prior art also discloses the extraction of polyphenols (such as CN109529404A), flavonoids (such as CN106317005A), lignin (such as CN105559095A), dietary fiber (such as CN105249482A), amino acids (such as CN108186429A), polysaccharides (such as CN108186429A) and polysaccharides (such as CN105669624A) etc., further improve the utilization rate of rose residue.

The development of pure natural organic fertilizers to replace organic chemical fertilizers will help greatly improve this situation.

Contents of the invention

Aiming at the deficiencies of the prior art, the present invention provides a rose dregs bio-organic fertilizer and its preparation method and application. The rose dregs are used as a fermentation substrate, and the bio-organic fertilizer is obtained after being fermented with a fermentation bacterial agent. The rose dregs organic fertilizer of the invention has the functions of improving soil, fixing nitrogen, decomposing phosphorus and potassium, and while replacing inorganic fertilizers, it can also improve crop quality, promote natural maturity of crops in advance and prolong the harvest period.

Technical scheme of the present invention is as follows:

A preparation method of rose dregs bio-organic fertilizer, the steps are as follows:

(1) Preparation of rose dregs fermentation substrate: take rose dregs, add water to adjust the water content of rose dregs to 70-80%, use brown sugar to adjust the total sugar content of rose dregs to 30-35%, after high-temperature sterilization Obtain rose pomace fermentation substrate;

(2) Activation of fermentation agent: fermentation agent comprises EM agent and salt-preserved halobacillus salinus (Halobacillus salinus) CGMCC NO.1.8606, uses 0.2% brown sugar water to respectively make EM agent and salt-preserved halobacillus ( Halobacillus salinus) CGMCC NO.1.8606 activation, 35 ~ 37 ° C activation culture 1 ~ 2d, the activated EM bacterial agent and salted bacillus (Halobacillus salinus) CGMCC NO.1.8606 were mixed to obtain the fermentation bacterial agent;

(3) Insert the fermented bacterial agent prepared in step (2) into the rose dregs fermented substrate prepared in step (1), seal the can, and ferment for 7 to 10 days at 30 to 40°C to obtain a fermented liquid; separate the fermented liquid from solid to liquid, The liquid is the rose dregs bio-organic liquid fertilizer, and the solid is dried and pulverized to become the rose dregs bio-organic solid fertilizer.

Preferably according to the present invention, in the step (1), water is added to adjust the water content of the rose dregs to 70%.

Preferably according to the present invention, brown sugar is used in step (1) to adjust the total sugar content of rose dregs to 30%.

Preferably according to the present invention, the EM bacterial agent described in step (2) and the salt-preserved halobacillus salinus (Halobacillussalinus) CGMCC NO.1.8606 are all bacterial powders, and the consumption of the EM bacterial agent is 0.04～ 0.10%, the dosage of the salt-preserved Halobacillus salinus CGMCC NO.1.8606 is 0.05-0.10% of the mass of rose pomace.

Further preferably, the dosage of the EM bacterial agent is 0.05% of the mass of rose pomace, and the dosage of the salted Halobacillus salinus CGMCC NO.1.8606 is 0.07% of the mass of rose pomace.

Preferably according to the present invention, in step (3), plate filter press is used for solid-liquid separation.

Preferably according to the present invention, the drying temperature in step (3) is 60-65°C.

The rose residue bio-organic liquid fertilizer prepared according to the above method.

The rose residue bio-organic solid fertilizer prepared according to the above method.

Application of the above-mentioned rose residue bio-organic fertilizer in crop cultivation and production.

The operation steps not described in detail in the present invention are conventional operations in the art.

In the present invention, the sugar content of rose dregs is adjusted in order to provide sufficient nutrients for the fermentation agent, improve fermentation activity, and produce more beneficial active ingredients; the sugar content of rose dregs is adjusted with brown sugar, mainly because The low price of brown sugar can reduce the production cost of rose dregs bio-organic fertilizer, but other components can also be used to adjust the sugar content of rose dregs, such as molasses. The present invention also uses 0.2% brown sugar water to activate the fermentation agent. On the basis of controlling the production cost, it also simplifies the operation steps of the activation of the strain. If a conventional strain medium, such as LB medium, can also be used Reach the purpose of activating bacterial classification, but cost is higher, and operation is more complicated.

Beneficial effect:

1. Rose dregs are rich in sugar, amino acids and other nutrients. Using rose dregs as a fermentation substrate to ferment and produce bio-organic fertilizers can effectively solve the recycling of rose dregs and further improve the utilization rate of rose dregs. The prepared bio-organic fertilizer can replace the inorganic fertilizer, reduce the usage of the inorganic fertilizer in crop production, and alleviate various problems caused by the excessive use of the inorganic fertilizer.

2. The present invention further optimizes the fermentation strain of rose dregs bio-organic fertilizer, which is to add salt-preserved halobacillus salinus (Halobacillus salinus) CGMCC NO.1.8606, EM bacterial agent and salt-preserved halobacillus (Halobacillus salinus) CGMCC NO.1.8606 interacts with each other. The bio-organic fertilizer prepared by the mixed bacterial agent can effectively increase the yield of crops, promote the early maturity of crops, significantly reduce the delivery time of crop maturity, and prolong the harvest period of crops. Improve the quality of crops, produce a variety of substances needed by plants, and also have the effect of improving soil, and play the role of nitrogen fixation, potassium and phosphorus dissolution.

3. The rose dregs bio-organic fertilizer prepared by the present invention includes rose dregs bio-organic liquid fertilizer and rose dregs bio-organic solid fertilizer. Those skilled in the art can reasonably choose to use different types of roses according to the types and growth stages of crops Residue bio-organic fertilizer.

Description of drawings

Fig. 1 is the picture of the cucumber harvested in Experimental Example 1.

Figure 2 is a picture of Chrysanthemum chrysanthemum harvested in Experimental Example 2.

Fig. 3 is the ginger picture of harvesting in Experimental Example 3.

Fig. 4 is the picture of the strawberry plant cultivated in Experimental Example 4.

FIG. 5 is a picture of corn plants planted in Experimental Example 5.

FIG. 6 is a picture of corn harvested in Experimental Example 5.

Figure 7 is a picture of the apples harvested in Experimental Example 6.

Figure 8 is a picture of pakchoi seedlings cultivated in Experimental Example 7.

Detailed ways

The technical solutions of the present invention will be further described below in conjunction with the examples, but the protection scope of the present invention is not limited thereto. The raw materials and medicines involved in the examples, unless otherwise specified, are conventional commercially available products in the art. The experimental operations involved in the examples, unless otherwise specified, are routine operations in the art. The percentages in the examples are all mass percentages unless otherwise specified.

EM bacterial agent is a commercially available bacterial agent, which can be purchased from Jining Jinshan Bioengineering Co., Ltd.

The rose residue of the present invention is purchased from Shandong Huamei Biotechnology Co., Ltd., and is a waste material obtained after a high-temperature cooking process.

The main nutrient content of above-mentioned rose dregs is as follows:

Table 1. Main nutrient content of rose pomace

nutrient content content Total sugar/% 25.5 Reducing sugar/% 8.3 Soluble sugar/% 9.0 Total acid (calculated as malic acid)/% 2.05 Ash/% 3.26

The halophilic bacillus involved in the examples are Halobacillus salinus CGMCC NO.1.8606 and Halobacillus salinus CGMCC NO.1.15964 respectively, both of which were purchased from China General Microorganism Culture Collection and Management Center.

Among them, Halobacillus salinus CGMCC NO.1.8606 was isolated from the sediments of the Pacific polymetallic contract area. It belongs to marine microorganisms and has certain halophilic characteristics.

Example 1:

A preparation method of rose dregs bio-organic fertilizer, the steps are as follows:

(1) Preparation of rose dregs fermentation substrate: take rose dregs, add water to adjust the water content of rose dregs to 70%, use brown sugar to adjust the total sugar content of rose dregs to 30%, and obtain rose dregs after high temperature sterilization Fermentation substrate;

(2) Activation of fermentation agent: fermentation agent includes EM agent and salt-preserved Halobacillus salinus (Halobacillus salinus) CGMCC NO.1.8606, EM agent and salt-preserved Halobacillus salinus (Halobacillus salinus) CGMCC NO.1.8606 are both Bacteria powder, the consumption of EM bacterial agent is 0.05% of the quality of rose dregs, the consumption of saline halobacillus salinus (Halobacillus salinus) CGMCC NO.1.8606 is 0.07% of the quality of rose dregs; EM microbial agent and salt-preserved Halobacillus salinus (Halobacillus salinus) CGMCC NO.1.8606 activation, 35 ° C activation culture 1d, the activated EM microbial agent and salt-preserved Halobacillus salinus (Halobacillus salinus) CGMCC NO.1.8606 were mixed, Obtain fermentation agent;

(3) Put the rose dregs fermentation substrate prepared in step (1) in a fermenter, insert the fermentation agent prepared in step (2), seal the can, and ferment at 37°C for 7 days to obtain a fermented liquid; the fermented liquid is pressed by plate The filter machine separates the solid from the liquid, the liquid is the rose dregs bio-organic liquid fertilizer, and the solid is dried and crushed at 60°C to become the rose dregs bio-organic solid fertilizer.

Example 2:

A preparation method of rose dregs bio-organic fertilizer, the steps are as follows:

(1) Preparation of rose dregs fermentation substrate: take rose dregs, add water to adjust the water content of rose dregs to 75%, use brown sugar to adjust the total sugar content of rose dregs to 32%, and obtain rose dregs after high temperature sterilization Fermentation substrate;

(2) Activation of fermentation agent: fermentation agent includes EM agent and salt-preserved Halobacillus salinus (Halobacillus salinus) CGMCC NO.1.8606, EM agent and salt-preserved Halobacillus salinus (Halobacillus salinus) CGMCC NO.1.8606 are both Bacteria powder, the consumption of EM bacterial agent is 0.06% of the quality of rose dregs, the consumption of saline halobacillus salinus (Halobacillus salinus) CGMCC NO.1.8606 is 0.08% of the quality of rose dregs; EM microbial agent and salt-preserved Halobacillus salinus (Halobacillus salinus) CGMCC NO.1.8606 activation, 36 ° C activation culture 1d, the activated EM microbial agent and salt-preserved Halobacillus salinus (Halobacillus salinus) CGMCC NO.1.8606 were mixed, Obtain fermentation agent;

(3) Put the rose dregs fermentation substrate prepared in step (1) in a fermenter, insert the fermented bacterial agent prepared in step (2), seal the can, and ferment at 35°C for 8 days to obtain a fermented liquid; the fermented liquid is pressed by plate The filter machine separates the solid from the liquid, the liquid is the rose dregs bio-organic liquid fertilizer, and the solid is dried and crushed at 60°C to become the rose dregs bio-organic solid fertilizer.

Example 3:

A preparation method of rose dregs bio-organic fertilizer, the steps are as follows:

(1) Preparation of rose dregs fermentation substrate: take rose dregs, add water to adjust the water content of rose dregs to 80%, use brown sugar to adjust the total sugar content of rose dregs to 35%, and obtain rose dregs after high temperature sterilization Fermentation substrate;

(2) Activation of fermentation agent: fermentation agent includes EM agent and salt-preserved Halobacillus salinus (Halobacillus salinus) CGMCC NO.1.8606, EM agent and salt-preserved Halobacillus salinus (Halobacillus salinus) CGMCC NO.1.8606 are both Bacteria powder, the consumption of EM bacterial agent is 0.07% of the quality of rose dregs, the consumption of saline halobacillus salinus (Halobacillus salinus) CGMCC NO.1.8606 is 0.10% of the quality of rose dregs; EM microbial agent and salt-preserved Halobacillus salinus (Halobacillus salinus) CGMCC NO.1.8606 activation, 37 ° C activation culture 1d, the activated EM microbial agent and salt-preserved Halobacillus salinus (Halobacillus salinus) CGMCC NO.1.8606 were mixed, Obtain fermentation agent;

(3) Place the rose dregs fermentation substrate prepared in step (1) in a fermenter, insert the fermented bacterial agent prepared in step (2), seal the can, and ferment at 30°C for 10 days to obtain a fermented liquid; the fermented liquid is pressed by plate The filter machine separates the solid from the liquid, the liquid is the rose dregs bio-organic liquid fertilizer, and the solid is dried and crushed at 65°C to become the rose dregs bio-organic solid fertilizer.

Comparative example 1:

A rose dregs bio-organic fertilizer, the preparation method of which is the same as that of Example 1, except that the fermentation agent is EM agent and salt-preserved halobacillus salinus (Halobacillus salinus) CGMCC NO.1.15964.

Apply the rose dregs bio-organic fertilizer prepared above to the cultivation or production of the following crops, and verify the application effect of the rose dregs bio-organic fertilizer.

Experimental Example 1: Cucumber Production

The rose dregs bio-organic fertilizer and commercially available urea prepared in Example 1 were applied to cucumber production to verify the effect of the rose dregs bio-organic fertilizer, and the planting soil was saline-alkali soil type.

The experimental group uses the rose dregs bio-organic solid fertilizer prepared in Example 1. The application rate of the rose dregs bio-organic solid fertilizer is 6kg/mu, and the fertilization method is to sow once after three days of cucumber planting, and then sow once after an interval of 15 days. The control group was administered with commercially available urea, and the dosage and method were the same as those of the experimental group.

The cucumber that planting obtains is shown in Figure 1, and wherein A picture is the experimental group that uses embodiment 1 rose residue bio-organic solid fertilizer, and B picture is the control group that uses commercially available urea, as can be seen from the figure, the cucumber of the experimental group The fruit is greener and fuller, and the sales grade has increased. Compared with the control group, the yield of cucumbers in the experimental group increased by more than 30%, the delivery time of cucumbers was reduced by more than 7 days, and the nutrients and microbial community structure in the soil of the experimental group were improved. The bio-organic fertilizer It has the functions of fixing nitrogen, dissolving potassium and dissolving phosphorus.

Experimental example 2: Chrysanthemum chrysanthemum production

The rose dregs bio-organic fertilizer and commercially available urea prepared in Example 1 were applied to the production of Chrysanthemum chrysanthemum, and the effect of the rose dregs bio-organic fertilizer was verified. The planting soil was a saline-alkali soil type.

The experimental group uses the rose dregs bio-organic solid liquid fertilizer prepared in Example 1. The application rate of the rose dregs bio-organic liquid fertilizer is 10kg/mu, and the fertilization method is that the foliage is sprayed once three days after Chrysanthemum chrysanthemum is planted, and after an interval of 15 days. Spray once. The control group was administered with commercially available urea, and the dosage and method were the same as those of the experimental group.

The Chrysanthemum chrysanthemum obtained by planting is shown in Figure 2, wherein the chrysanthemum chrysanthemum on the left is the experimental group using the rose residue bio-organic liquid fertilizer in Example 1, and the chrysanthemum chrysanthemum on the right is the control group using commercially available urea. It can be seen from the figure that the experimental group The chrysanthemum chrysanthemum in the control group was fuller, and the sales level increased, while the chrysanthemum chrysanthemum in the control group had hollow stems, and the quality was not good. Compared with the control group, the yield of chrysanthemum chrysanthemum in the experimental group increased by more than 30%, and the shipping time of chrysanthemum chrysanthemum matured by more than 5 days, and the nutrients and microbial community structure in the soil of the experimental group were improved. The bio-organic fertilizer It has the functions of fixing nitrogen, dissolving potassium and dissolving phosphorus.

Experimental Example 3: Ginger Production

The rose dregs bio-organic fertilizer prepared in Example 1 and Comparative Example 1 was applied to ginger production to verify the effect of the rose dregs bio-organic fertilizer, and the planting area was Laiwu ginger production area.

The experimental group uses the rose dregs bio-organic solid fertilizer prepared in Example 1. The application rate of the rose dregs bio-organic solid fertilizer is 8kg/mu, and the fertilization method is to sow once three days after the ginger is planted, and then sow once after an interval of 15 days. The control group was applied the rose residue bio-organic solid fertilizer prepared in Comparative Example 1, and the application amount and application method were the same as those of the experimental group.

The ginger that planting obtains is shown in Figure 3, and wherein left side ginger is the experimental group that uses embodiment 1 rose slag bio-organic solid fertilizer, and right side ginger is the control group that uses comparative example 1 rose slag bio-organic solid fertilizer, by As can be seen from the figure, the ginger tendons in the experimental group decreased, the quality was better, and the sales level increased. Compared with the control group, the yield of ginger in the experimental group increased by more than 15%, the sweetness increased, and the delivery time for ginger maturity was reduced by more than 3 days.

Experimental Example 4: Strawberry Production

The rose residue bio-organic fertilizer prepared in Example 1 and Comparative Example 1 was applied to strawberry production to verify the effect of the rose residue bio-organic fertilizer.

The experimental group uses the rose dregs bio-organic liquid fertilizer prepared in Example 1. The sowing amount of the rose dregs bio-organic liquid fertilizer is 10kg/mu, and the fertilization method is that the strawberry seedlings are sprayed once on the leaves after three days after planting, and then sprayed after an interval of 15 days once. The control group was applied the rose residue bio-organic liquid fertilizer prepared in Comparative Example 1, and the application amount and application method were the same as those of the experimental group.

The strawberry seedling that cultivates alive is as shown in Figure 4, and wherein the left figure is the control group that uses comparative example 1 rose slag bio-organic liquid fertilizer, and the right figure is the experimental group that uses embodiment 1 rose slag bio-organic liquid fertilizer, by Fig. It can be seen that the root system of the surviving strawberry seedlings cultivated in the experimental group is more developed, the seedling stems are short and strong, and the quality is high, and the survival rate of the seedlings reaches more than 90%. Compared with the control group, the strawberry yield of the experimental group increased by more than 20%, the aroma was strong, the anthocyanin content was significantly increased, and the delivery time of strawberry ripening was reduced by more than 5 days.

Experimental Example 5: Corn Production

The rose dregs bio-organic fertilizer prepared in Example 1 and commercially available urea were applied to corn planting to verify the effect of the rose dregs bio-organic fertilizer.

The experimental group applied the rose dregs bio-organic solid fertilizer prepared in Example 1. The application rate of the rose dregs bio-organic solid fertilizer was 10 kg/mu, and the fertilization method was as a base fertilizer when corn was planted. The control group was administered with commercially available urea, and the dosage and method were the same as those of the experimental group.

The corn of planting is as shown in Figure 5, and wherein the left picture is the experimental group that uses embodiment 1 rose residue bio-organic solid fertilizer, and the right picture is the control group that uses urea, as can be seen from the figure, the corn plant leaf growth of experimental group Orderly, the ventilation effect is good, the corn leaves of the control group are disorderly and the ventilation effect is poor. Ventilation effect will affect pollination and fruit growth. After the corns matured, 50 corns were picked at random. As shown in Figure 6, the upper corns are the corns of the control group, and the lower corns are the corns of the experimental group. Full, the output increased significantly.

Experimental Example 6: Apple Production

The rose dregs bio-organic fertilizer prepared in Example 1 and Comparative Example 1 was applied to apple production to verify the effect of the rose dregs bio-organic fertilizer.

The experimental group applied the rose dregs bio-organic solid fertilizer prepared in Example 1, and the application rate of the rose dregs bio-organic solid fertilizer was 10 kg/mu, and was applied at the bud stage. The control group was applied the rose residue bio-organic solid fertilizer prepared in Comparative Example 1, and the application amount and application method were the same as those of the experimental group.

The apple of harvest is as shown in Figure 7, and wherein left side apple is the experimental group that uses embodiment 1 rose slag bio-organic solid fertilizer, and right side apple is the control group that uses the rose slag bio-organic solid fertilizer that comparative example 1 prepares, It can be seen from the figure that the apple seeds of the control group were poorly developed, and the cracking of the core easily caused the infection of bacteria, and the pulp was loose and soft; while the apple tissue structure of the experimental group was dense, and the fruit quality of the same volume was too large. Compared with the control group, the apples harvested by the experimental group have higher content of superoxide dismutase and other nutrients, better taste, crisp and palatable, high sugar content and strong aroma, higher carotene and anthocyanin content, and delicate appearance. Coloring is good.

Experimental Example 7: Cultivation of Chinese Cabbage

The rose residue bio-organic fertilizer prepared in Example 1 and Comparative Example 1 was applied to the cultivation of Chinese cabbage seedlings to verify the effect of the rose residue bio-organic fertilizer.

Plant pak choi with flower pots, sow 50 pak choy seeds in each pot, and apply fertilizer when spreading the seeds, wherein, the experimental group uses the rose residue bio-organic solid fertilizer prepared in Example 1, and the application rate is 100g/pot, and the control group 1. The rose residue bio-organic solid fertilizer prepared in Comparative Example 1 was applied. The commercially available urea was applied to the control group 2. The application amounts of the control group 1 and the control group 2 were the same as those of the experimental group.

The emergence of pakchoi seedlings is shown in Figure 8, wherein the left pakchoi is the control group 2 using urea, the middle pakchoi is the control group 1 using the rose residue bio-organic solid fertilizer of comparative example 1, and the right pakchoi is The experimental group using the rose residue bio-organic solid fertilizer of embodiment 1. As can be seen from the figure, the growth of the pakchoi seedlings in the experimental group is more vigorous, the germination rate of the pakchoi seedlings in the experimental group is 80-90%, the leaves are thick, and the root system is developed. The germination rate of the pakchoi seedlings in the control group 1 is 50%, and the control The germination rate of the pakchoi seedlings in group 2 was 60%.

As can be seen from the above experimental examples, the rose dregs bio-organic fertilizer prepared by the present invention can effectively replace inorganic fertilizers, and can effectively increase crop yields, promote early maturity of crops, significantly reduce the shipping time of crop maturity, and prolong the harvest period of crops , improve crop quality.

Claims (10)

1. A preparation method of a rose residue bio-organic fertilizer is characterized by comprising the following steps:

(1) Preparing a rose dreg fermentation substrate: adding water into rose dregs to adjust the water content of the rose dregs to 70-80%, adopting brown sugar to adjust the total sugar content of the rose dregs to 30-35%, and sterilizing at high temperature to obtain a rose dreg fermentation substrate;

(2) Activating the fermentation inoculum: the fermentation inoculum comprises EM inoculum and halophilic bacillus (Halobacillus salinus) CGMCC NO.1.8606, the EM inoculum and the halophilic bacillus (Halobacillus salinus) CGMCC NO.1.8606 are respectively activated by using 0.2% brown sugar water, activated and cultured for 1-2 days at 35-37 ℃, and the activated EM inoculum and the activated halophilic bacillus (Halobacillus salinus) CGMCC NO.1.8606 are mixed to obtain the fermentation inoculum;

(3) Inoculating the fermentation microbial inoculum prepared in the step (2) into the rose residue fermentation substrate prepared in the step (1), sealing the tank, and fermenting at 30-40 ℃ for 7-10 days to obtain fermentation liquor; and (3) performing solid-liquid separation on the fermentation liquor, wherein the liquid is the rose dreg bio-organic liquid fertilizer, and the solid is dried and crushed to obtain the rose dreg bio-organic solid fertilizer.

2. The method according to claim 1, wherein the water content of the rose dregs in the step (1) is adjusted to 70%.

3. The method according to claim 1, wherein the total sugar content of the rose dregs in the step (1) is adjusted to 30% by brown sugar.

4. The preparation method according to claim 1, wherein the EM microbial agent and the halophilic Bacillus salinus (Halobacillus salinus) CGMCC NO.1.8606 in the step (2) are bacterial powder, the amount of the EM microbial agent is 0.04-0.10% of the mass of the rose residue, and the amount of the halophilic Bacillus salinus (Halobacillus salinus) CGMCC NO.1.8606 is 0.05-0.10% of the mass of the rose residue.

5. The preparation method according to claim 4, wherein the EM microbial inoculum is used in an amount of 0.05% by mass of the rose residue, and the Bacillus salinus (Halobacillus salinus) CGMCC NO.1.8606 is used in an amount of 0.07% by mass of the rose residue.

6. The production process according to claim 1, wherein in the step (3), solid-liquid separation is carried out by plate-type pressure filtration.

7. The method according to claim 1, wherein the temperature for the drying in the step (3) is 60 to 65 ℃.

8. The bio-organic liquid fertilizer for rose dregs, which is prepared by the preparation method of claim 1.

9. The bio-organic solid fertilizer for rose dregs, which is prepared by the preparation method according to claim 1.

10. Use of the rose dreg bioorganic liquid fertilizer of claim 8 or the rose dreg bioorganic solid fertilizer of claim 9 in crop cultivation and production.

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