CN115251380A - Method and application of preparing antioxidant from arbor tissue by alcohol soaking method - Google Patents

Abstract

The invention relates to a method for preparing an antioxidant from arbor tissues by adopting an alcohol soaking method and application thereof. Taking crushed arbor tissue powder as a raw material; adding arbor tissue powder into a soaking tank, injecting alcohol and a permeation aid, and soaking; extracting the alcohol-soaked arbor tissue powder with supercritical or subcritical conditions at 30-75 deg.C and 2-300atm pressure with carbon dioxide as extractant; after supercritical or subcritical extraction, the component containing the arbor tissue extract, the alcohol and the extractant is defined as an arbor tissue antioxidant mixture, the arbor tissue antioxidant mixture is separated and purified to obtain a pure arbor tissue extract or arbor oil, and the purified arbor tissue extract or arbor oil is defined as an arbor oil mixture. Compared with the prior art, the method is simple and easy to implement, and has stronger industrialized practicability and operability. When the antioxidant is used in cosmetics and foods, no antioxidant needs to be added in the cosmetics and foods.

Description

Method and Application of Antioxidant Prepared from Arbor Tissue by Alcohol Soaking Method

technical field

The invention belongs to the field of separation and extraction technology for extracting active ingredients from natural plants to prepare fine chemicals, and in particular relates to a method and application for preparing antioxidants from arbor tissues by using an alcohol soaking method.

Background technique

At present, the antioxidants used in the field of cosmetics and medicine generally use chemically synthesized antioxidants, which have clear chemical structures and clear physical and chemical properties. However, most of these antioxidants are food-grade, and the shelf life of food-grade antioxidants is generally relatively short. , the longer ones are generally 18 months, and most of them are kept in cold storage. However, the shelf life of products required in the field of cosmetics or medicine is generally more than three years, and most of them are stored at room temperature. Most of them are emulsified or oil-water mixtures, and the risk of being easily oxidized is high. Therefore, general food-grade antioxidants are not very suitable for cosmetics. .

Arbor refers to tall trees with independent trunks from the roots, and the trunk and crown are clearly distinguished. A woody plant with an upright trunk, usually six to tens of meters high. Arbor tissue generally refers to macroscopic tissues such as its seeds, leaves, roots, resin, resin, etc. No matter what kind of arbor, its specific tissues are rich in unsaturated fatty acids such as retinol, ascorbic acid, tocopherol, oleic acid, linoleic acid, linolenic acid, flavonoids, tannic acid, paeonol, cinnamon phenol, polyphenols, etc. Substances such as phenol and astaxanthin. The commonality of these substances is that they are easy to react with oxidants, and some will react with trace oxygen in the air or in the system under low temperature conditions. These are very effective naturally occurring antioxidants.

At present, there are almost no literature reports on the study of arbor tissue oil, let alone the research and development of the production process of arbor tissue antioxidants.

In addition, with the continuous improvement of extraction and analysis techniques in recent years, supercritical or subcritical alcohol extraction methods are mostly used in the field of phytochemistry, but there are no reports on the application of these methods to extract antioxidants from tree tissues.

Contents of the invention

The object of the present invention is to provide a method and application of preparing antioxidant from arbor tissue by alcohol soaking method.

The invention adopts supercritical or subcritical extraction alcohols to soak arbor tissue to obtain antioxidants, and the antioxidants can be applied to technical fields such as cosmetics, food or health products.

The purpose of the present invention can be achieved through the following technical solutions:

The invention provides a method for preparing antioxidants from arbor tissue by alcohol soaking method, specifically, using supercritical or subcritical alcohol extraction method to prepare antioxidants from arbor tissue, comprising the following steps: 30-75°C, under the pressure of 2-300atm, the extraction agent is carbon dioxide, supercritical or subcritical extraction of arbor tissue powder soaked in alcohols.

In one embodiment of the present invention, the method for preparing antioxidants from arbor tissue by the alcohol soaking method specifically includes the following steps:

The crushed arbor tissue powder is used as raw material;

Add the arbor tissue powder into the soaking tank, inject alcohols and penetration aids, and soak;

At 30-75°C, under the pressure of 2-300atm, the extraction agent is carbon dioxide, and the arbor tissue powder soaked in alcohol is supercritical or subcritical extraction;

After supercritical or subcritical extraction, after supercritical or subcritical extraction, the components containing arbor tissue extracts, alcohols and extractants are defined as arbor tissue antioxidant mixture, and arbor tissue antioxidant mixture is separated and purified to obtain pure The arbor tissue extract or arbor oil, and the purified arbor tissue extract or arbor oil are defined as an arbor oil mixture.

In one embodiment of the present invention, the arbor tissue powder is obtained by pulverizing the arbor tissue with a pulverizer after removing impurities, and sieving the arbor tissue. The arbor tissue is obtained from seeds, leaves, resin, bark and roots. one or several.

In one embodiment of the present invention, the arbor tissue is selected from walnut tree, sweet almond tree, American hickory tree, plateau wild walnut tree, black walnut tree, hard-shelled walnut tree, ginkgo tree, hawthorn tree, magnolia tree, white birch Tree, pine, red pine, cedar, five-leaved pine, podocarpus, masson pine, scotch pine, dead pine, Italian pine, tabula pine, longleaf pine, Mediterranean cypress, cypress, juniper, juniper, Japanese Cypress, Juniper, Mexican Juniper, Argan, Radiata Pine, Paper Tree, Lotus Magnolia, Baobab, Bitter Tree, Shea, Camphor, Savoy Magnolia, Banyan, Catalpa, Hemp Walnut trees, Keppel walnut trees, black walnut trees, heart-shaped walnut trees, summer walnut trees, autumn walnut trees, light walnut trees, iron walnut trees, catalpa, Qinghai-Tibet plateau walnut trees. In particular, hickory, cedar, juniper, baobab, fall walnut, pine, and especially hickory.

In one embodiment of the present invention, the particle size of the arbor tissue powder is 2-150 mm, preferably 2-20 mm, most preferably 10 mm.

In one embodiment of the present invention, the alcohols are selected from ethanol, propylene glycol, glycerin, 1,3 propylene glycol, 1,3 butanediol, 2,3 butanediol, 1,4 butanediol, carbitol , one or more of benzyl alcohol or phenylethyl alcohol; preferably ethanol, propylene glycol, glycerin, most preferably ethanol;

In one embodiment of the present invention, the penetration enhancer is selected from tocopherol, dimethyl sulfone, frankincense extract, paeonol or food-grade chemically synthesized antioxidant. The penetration aid is preferably tocopherol, dimethyl sulfone, chemically synthesized antioxidant, most preferably dimethyl sulfone.

In one embodiment of the present invention, the weight ratio of the alcohols and penetration aids to the arbor tissue powder is 2-8:1, preferably 2-4:1, most preferably 3.5:1.

In one embodiment of the present invention, the weight ratio of alcohols and penetration aids is 1-100:1, preferably 10-80:1, most preferably 50:1.

In one embodiment of the present invention, the soaking conditions are soaking time 2h-5h, most preferably 3 hours; soaking temperature 45-65°C, most preferably 55°C.

In one embodiment of the present invention, during extraction, the pressure is preferably 1.5-90 atm, most preferably 50 atm.

In one embodiment of the present invention, after desolvating the obtained alcohols under reduced pressure, the yield of arbor tissue antioxidants is 1.8-10.8%.

The present invention also provides the antioxidant prepared by the above method. Antioxidants are of good quality and high yield.

The antioxidant prepared by the above method was analyzed by GC-MS, and the content of oleic acid in the antioxidant was ≥ 5%, the content of linoleic acid ≥ 30%, the content of linolenic acid ≥ 50%, and the content of greater than 13% of eicosan unsaturated fatty acids. Alcohol content ≥ 0.5%, tocopherol content ≥ 0.55%, phytosterol content ≥ 2%, flavonoid polypeptide content ≥ 5%.

The supercritical or subcritical product prepared by the above method, which contains arbor tissue extract, alcohols and extractant, is defined as arbor tissue antioxidant mixture. The resultant does not require isolation and purification to be used directly as an antioxidant, especially in cosmetics and food. Moreover, the above-mentioned obtained can also be separated and purified to obtain pure arbor tree tissue extract or arbor tree oil. Purified arbor tissue extract or arbor oil, defined as arbor oil mixture, can be reconstituted into oil and used as an antioxidant additive.

The present invention also provides the application of the arbor tissue antioxidant mixture and arbor oil mixture prepared by the method of the present invention, and the arbor tissue antioxidant mixture and arbor oil mixture also have the following purposes:

1. Direct consumption as a health product: the extracted arbor tissue antioxidant mixture and arbor oil mixture can be eaten directly after being refined.

2. Make functional blending oil: combine with other edible base oils to make a blending oil with anti-oxidation and free radical elimination effects.

The method provided by the invention adopts alcohol soaking method to prepare antioxidant from arbor tissue, and the key points are as follows:

One, the selection of temperature, the emphases of the present invention are the selection of temperature, in extracting, adopt the mode of supercritical or subcritical to usually be able to improve extraction rate, but the selection of temperature is key point, and the application proves by many experimental studies , the temperature used is 55-65°C, most preferably 55°C, the extraction effect is the best.

Second, the selection of alcohols for extraction has been proved by many experiments and studies that ethanol, propylene glycol, glycerin, 1,3 propylene glycol, 1,3 butanediol, 2,3 butanediol, 1,4 butanediol, Carbitol, benzyl alcohol or phenylethyl alcohol can effectively extract antioxidants from tree tissues, especially ethanol, propylene glycol, glycerin, and ethanol is particularly preferred as the extraction alcohol, and the extraction effect is better.

Third, the choice of extracting penetration aids has been proved by many experimental studies that the use of tocopherol, dimethyl sulfone, frankincense extract, paeonol, and food-grade chemically synthesized antioxidants can effectively extract antioxidants from tree tissues. Oxidants, especially tocopherol, dimethyl sulfone, and chemically synthesized antioxidants, most preferably dimethyl sulfone, have better extraction effects.

Fourth, the selection of the weight ratio of alcohols and penetration aids to raw materials. It has been proved by research that when the weight ratio of alcohols and penetration aids to raw materials is 2-8:1, the product can be effectively extracted, especially the weight ratio Ratio of 2-4:1, especially 3.5:1, can be extracted more efficiently.

Fifth, when the molar ratio of alcohols and penetration aids is 1-100:1, the product can be effectively extracted, especially when the weight ratio is 10-80:1, especially when it is 50:1, it can more effectively extract extract.

Sixth, the choice of soaking temperature. Generally speaking, according to the different properties of the raw materials, the soaking temperature varies greatly. After many experiments and researches, it has been proved that when the above process is used for extraction, the soaking temperature is 45-65 ° C, especially Antioxidants in arbor tissues can be effectively extracted at 55°C.

Its seven, the selection of arbor tissue, generally speaking, according to the difference of the performance of arbor tissue itself, the difference is relatively big, proves through many experimental studies, when adopting above-mentioned technology to extract walnut tree, sweet almond tree, American hickory tree, plateau Wild walnut tree, black walnut tree, hard-shelled walnut tree, ginkgo tree, hawthorn tree, magnolia tree, white birch tree, pine tree, red pine tree, cedar tree, five-leaf pine tree, podocarpus tree, masson pine tree, scabbard pine tree, dead pine tree, Italian pine, pitch pine, longleaf pine, Mediterranean cypress, cypress, juniper, juniper, Japanese hinoki, juniper, Mexican juniper, arborina, radiata pine, mulberry, lotus magnolia, monkey Bread tree, bitter oil tree, avocado tree, camphor tree, wrinkled magnolia tree, banyan tree, catalpa, hemp walnut tree, Keppel walnut tree, black walnut tree, heart-shaped walnut tree, summer walnut tree, autumn walnut tree, light walnut tree, iron walnut tree, catalpa tree, Qinghai-Tibet plateau walnut tree. In particular, hickory, cedar, juniper, baobab, fall walnut, pine, and especially hickory. The yield of extracting antioxidant by supercritical or subcritical alcohol immersion method is between 1.8-10.8%.

In addition, although the solvent immersion method is a conventional extraction method, there are very few studies on arbor tissue, and there is no report on the use of alcohols in arbor tissue extraction. Compared with the prior art, the present invention has the following positive effects:

Antioxidants can be effectively extracted from arbor tissue by supercritical or subcritical alcohol extraction method, the method of the present invention is simple and easy, and because the raw material of arbor tissue is natural traditional edible plant, the raw material is abundant and cheap, and the obtained product is pollution-free , no toxic and side effects, safe and practical, so the method of the present invention has strong industrial practicability and operability. When used in cosmetics and food, there is no need to add additional antioxidants to cosmetics and food.

Detailed ways

The present invention provides a kind of method that adopts alcohol soaking method to prepare antioxidant from arbor tissue, specifically comprises the following steps:

The crushed arbor tissue powder is used as raw material;

Add the arbor tissue powder into the soaking tank, inject alcohols and penetration aids, and soak;

At 30-75°C, under the pressure of 2-300atm, the extraction agent is carbon dioxide, and the arbor tissue powder soaked in alcohol is supercritical or subcritical extraction;

After supercritical or subcritical extraction, after supercritical or subcritical extraction, the components containing arbor tissue extracts, alcohols and extractants are defined as arbor tissue antioxidant mixture, and arbor tissue antioxidant mixture is separated and purified to obtain pure The arbor tissue extract or arbor oil, and the purified arbor tissue extract or arbor oil are defined as an arbor oil mixture.

In one embodiment of the present invention, the arbor tissue is selected from walnut tree, sweet almond tree, American hickory tree, plateau wild walnut tree, black walnut tree, hard-shelled walnut tree, ginkgo tree, hawthorn tree, magnolia tree, white birch Tree, pine, red pine, cedar, five-leaved pine, podocarpus, masson pine, scotch pine, dead pine, Italian pine, tabula pine, longleaf pine, Mediterranean cypress, cypress, juniper, juniper, Japanese Cypress, Juniper, Mexican Juniper, Argan, Radiata Pine, Paper Tree, Lotus Magnolia, Baobab, Bitter Tree, Shea, Camphor, Savoy Magnolia, Banyan, Catalpa, Hemp Walnut trees, Keppel walnut trees, black walnut trees, heart-shaped walnut trees, summer walnut trees, autumn walnut trees, light walnut trees, iron walnut trees, catalpa, Qinghai-Tibet plateau walnut trees. In particular, hickory, cedar, juniper, baobab, fall walnut, pine, and especially hickory.

In one embodiment of the present invention, the particle size of the arbor tissue powder is 2-150 mm, preferably 2-20 mm, most preferably 10 mm.

In one embodiment of the present invention, the alcohols are selected from ethanol, propylene glycol, glycerin, 1,3 propylene glycol, 1,3 butanediol, 2,3 butanediol, 1,4 butanediol, carbitol , one or more of benzyl alcohol or phenylethyl alcohol; preferably ethanol, propylene glycol, glycerin, most preferably ethanol;

In one embodiment of the present invention, the penetration enhancer is selected from tocopherol, dimethyl sulfone, frankincense extract, paeonol or food-grade chemically synthesized antioxidant. The penetration aid is preferably tocopherol, dimethyl sulfone, chemically synthesized antioxidant, most preferably dimethyl sulfone.

In one embodiment of the present invention, the weight ratio of the alcohols and penetration aids to the arbor tissue powder is 2-8:1, preferably 2-4:1, most preferably 3.5:1.

In one embodiment of the present invention, the weight ratio of alcohols and penetration aids is 1-100:1, preferably 10-80:1, most preferably 50:1.

In one embodiment of the present invention, the soaking conditions are soaking time 2h-5h, most preferably 3 hours; soaking temperature 45-65°C, most preferably 55°C.

In one embodiment of the present invention, during extraction, the pressure is preferably 1.5-90 atm, most preferably 50 atm.

In one embodiment of the present invention, after desolvating the obtained alcohols under reduced pressure, the yield of arbor tissue antioxidants is 1.8-10.8%.

In the antioxidant prepared by the above method, the arbor tissue content is more than 1.8%, and the antioxidant has good quality and high yield.

Table 1 shows the yields of antioxidants obtained when the weight ratios of different alcoholic arbor tissue powders used for extraction in this application are studied.

The yield of the antioxidant obtained when table 1 adopts the weight ratio of different alcohols arbor tissue powder to extract

In Table 1, the pressure used in this extraction method is 50atm, the particle size of the tree tissue powder is 20mm, the algae is hickory, the soaking time is 2 hours, the soaking temperature is 75°C, and the penetration aid is a chemically synthesized food antioxidant. , The ratio of penetration aid to alcohol is 1:30.

The yields of antioxidants obtained during extraction with different penetration aids are shown in Table 2.

The productive rate of the antioxidant obtained when table 2 adopts different penetration aids to extract

In Table 2, the weight ratio of alcohols to raw materials used in this extraction method is 3:1, the crushed particle size of arbor tissue powder is 20mm, the soaking time is 2.5 hours, the extraction pressure is 70atm, and the temperature is 50°C.

Table 3: Yields of antioxidants obtained using extraction at different temperatures

In Table 3, the weight ratio of alcohols and arbor tissue powder used in this extraction method is 3:1, the crushed particle size of arbor tissue powder is 20 mm, the soaking time is 3 hours, and the extraction pressure is 70 atm. The penetration aid is tocopherol , The ratio of penetration aid to alcohol is 1:4.

In the extraction technical scheme provided by the present invention, GC-MS analysis is carried out to the antioxidant prepared by the above method, the content of oleic acid in the antioxidant ≥ 5%, the content of linoleic acid ≥ 30%, the content of linolenic acid ≥ 50%, more than two Ten-carbon unsaturated fatty acid 13%, retinol content ≥ 0.5%, tocopherol content ≥ 0.55%, phytosterol content ≥ 2%, flavonoid polypeptide content ≥ 5%. The supercritical or subcritical product prepared by the above method contains arbor tissue extract, alcohols and extractant, which we define as arbor tissue antioxidant mixture. The resultant does not require isolation and purification to be used directly as an antioxidant, especially in cosmetics and food. Moreover, the above-mentioned obtained can also be separated and purified to obtain pure arbor tree tissue extract or arbor tree oil. Purified arbor tissue extract or arbor oil, defined as arbor oil mixture, can be reconstituted into oil and used as an antioxidant additive.

The present invention will be described in detail below in conjunction with specific embodiments.

(1) Preparation Examples

Example 1

Adopt intermittent supercritical extraction tank group to extract soaked arbor tissue powder: take 100 grams of pulverized arbor tissue powder as raw material, the pulverized particle size is 20mm, add leaching tank, inject 400 grams of soaking ethanol, penetration aid dimethyl sulfone 2 grams, soaking time 3h, soaking temperature 75 ℃. After the leaching, the soaked material in the leaching tank was subjected to supercritical extraction, and 410.6 grams of the obtained arbor tissue antioxidant agent was subjected to desolvation under reduced pressure to obtain 8.6 grams of arbor oil mixture, with a yield of 8.6%. Arbor tissue powder for walnut trees.

Example 2

Adopt intermittent supercritical extraction tank group to extract soaked arbor tissue powder: take 100 grams of pulverized algae powder as raw material, the pulverized particle size is 90mm, add leaching tank, inject 200 grams of soaked camellia oil, 5 grams of tocopherol as a penetration aid, The soaking time was 20min, and the soaking temperature was 30°C; after the leaching, the soaked material in the leaching tank was subjected to supercritical extraction, and 214.0 grams of the obtained arbor tissue antioxidant was subjected to desolvation under reduced pressure to obtain 9 grams of arbor oil mixture, with a yield of 9%. Arbor tissue powder is Juniper.

Example 3

Batch-type supercritical extraction tanks are used to extract the soaked arbor tissue powder: 100 grams of crushed arbor tissue powder is used as a raw material, and the crushed particle size is 20 mm. Add the leaching tank, inject 300 grams of soaked propylene glycol, and synthesize antioxidant 2 as a penetration aid. grams, soaking time 3h, soaking temperature 75 ℃. After the leaching, the soaked material in the leaching tank was subjected to supercritical extraction, and 310.8 grams of the obtained arbor tissue antioxidant agent was subjected to desolvation under reduced pressure to obtain 8.8 grams of arbor oil mixture, with a yield of 8.8%. The arbor tissue powder is hickory.

Example 4

Use intermittent supercritical extraction tanks to extract soaked arbor tissue powder: use 100 grams of crushed algae powder as raw material, with a particle size of 50 mm, add to the extraction tank, inject 200 grams of soaked camellia oil, and 5 grams of paeonol as a penetration aid , immersion time 120min, immersion temperature 76 ℃; after leaching, the immersion in the leaching tank was carried out to supercritical extraction, and the obtained arbor tissue antioxidant 214.0 grams was subjected to decompression precipitation to obtain 9 grams of arbor oil mixture, yield 9%. Arbor tissue powder for pine.

Example 5-39

The process is to use a batch-type supercritical extraction tank group to extract soaked tree tissue powder: 100 grams of crushed tree tissue powder is used as raw material, and the crushed particle size is 20 mm. The pressure is 50atm, the particle size of arbor tissue powder is 55mm, the soaking time is 4.5 hours, the soaking temperature is 75°C, the penetration aid is dimethyl sulfone, and the ratio of penetration aid to alcohol is 1:30. The difference is that the algae species are different, and the arbor tissues used are walnut tree, sweet almond tree, American hickory tree, plateau wild walnut tree, black walnut tree, hard-shelled walnut tree, ginkgo tree, hawthorn tree, magnolia tree, white birch tree, and pine tree. , red pine, cedar, five-leaf pine, podocarpus, masson pine, scabbard pine, dead pine, Italian pine, tabula pine, longleaf pine, Mediterranean cypress, cypress, juniper, juniper, Japanese hinoki, American juniper, Mexican juniper, argan, radiata pine, paper mulberry, lotus magnolia, baobab, bitter oil, avocado, camphor, wrinkled magnolia, banyan, catalpa, hemp walnut, Keppel walnut tree, black walnut tree, heart-shaped walnut tree, summer walnut tree, autumn walnut tree, light walnut tree, iron walnut tree, catalpa tree, Qinghai-Tibet plateau walnut tree. The yield (unit %) is specifically shown in Table 4.

Table 4: Yields of antioxidants obtained when different arbors were used for extraction

In Table 4, the pressure used in this extraction method is 50 atm, the particle size of the arbor tissue powder is 55 mm, the algae is hickory, the soaking time is 4.5 hours, the soaking temperature is 75 ° C, the penetration aid is dimethyl sulfone, and the penetration aid The ratio of solvent to alcohol is 1:30.

(2) Antioxidant examples of arbor oil mixture, as shown in Table 5.

Examples 40-55

Mix the arbor oil mixture obtained in Examples 1-39, get 1.8 grams of the arbor oil mixture after mixing, and the fat is 99.8 grams. Oils and fats are ethanol, propylene glycol, glycerin, 1,3 propylene glycol, 1,3 butanediol, 2,3 butanediol, 1,4 butanediol, carbitol, benzyl alcohol, phenylethyl alcohol, lard, tallow , horse oil, snake oil, sheep oil, fish oil. The Metrohm Oil Oxidation Stability Tester tested the anti-oxidation stability time at 110°C with and without arbor oil mixture:

Table 5 The antioxidant stability time (110°C) of each oil without adding arbor oil mixture and adding arbor oil mixture

Examples 56-78

The antioxidant properties of single arbor oil mixture and composite arbor oil mixture are shown in Table 6.

Experimental method is the same as embodiment 40-55.

Pure Arbor Oils are Hickory, Cedar, Juniper, Baobab, Fall Walnut, Pine. Examples 56-61 were obtained.

The composite arbor organization is walnut tree, sweet almond tree, American hickory tree, highland wild walnut tree, black walnut tree, hard shell walnut tree, ginkgo tree, hawthorn tree, magnolia tree, white birch tree, pine tree, red pine tree, cedar tree, Five-leaf pine, podocarpus, masson pine, scabbard pine, dead pine, Italian pine, pitch pine, longleaf pine, Mediterranean cypress, cypress, juniper, juniper, Japanese hinoki, juniper, Mexican juniper , thorn tree, radiata pine tree, paper tree, lotus magnolia tree, baobab tree, bitter oil tree, avocado tree, camphor tree, wrinkled magnolia tree, banyan tree, catalpa, hemp walnut tree, Keppel walnut tree, black walnut Randomly select 3-5 kinds of arbor oil mixtures from walnut tree, heart-shaped walnut tree, summer walnut tree, autumn walnut tree, light walnut tree, iron walnut tree, catalpa tree, and Qinghai-Tibet plateau walnut tree to obtain embodiment 62-70.

The added amount of all arbor oil mixtures is 1.8 grams, and the fat is 99.8 grams, and the fat is olive oil.

Table 6 Antioxidant performance of single arbor oil mixture and compound arbor oil mixture

(3) Examples of antioxidant research on arbor tissue antioxidants, as shown in Table 7.

Examples 79-94

Mix the arbor tissue antioxidant mixture obtained in Examples 1-39, get 2 grams of the arbor tissue antioxidant mixture after mixing, and the oil is 98 grams. The oils are camellia oil, jojoba oil, squalane, soybean oil, almond oil, avocado oil, safflower oil, walnut oil, wheat germ oil, olive oil, butter, horse oil, snake oil, sheep oil, fish oil . The antioxidant stability time at 110°C with and without arbor oil mixture was tested by Metrohm Oxidation Stability Tester:

Table 7 The antioxidant stability time (110°C) of each oil without adding arbor oil mixture and adding arbor oil mixture

Examples 95-115

The antioxidant properties of single arbor oil mixture and compound arbor oil mixture are shown in Table 8.

Experimental method is the same as embodiment 79-94.

Pure Arbor Oils are Hickory, Cedar, Juniper, Baobab, Fall Walnut, Pine. Examples 95-100 were obtained.

The composite arbor organization is walnut tree, sweet almond tree, American hickory tree, highland wild walnut tree, black walnut tree, hard shell walnut tree, ginkgo tree, hawthorn tree, magnolia tree, white birch tree, pine tree, red pine tree, cedar tree, Five-leaf pine, podocarpus, masson pine, scabbard pine, dead pine, Italian pine, pitch pine, longleaf pine, Mediterranean cypress, cypress, juniper, juniper, Japanese hinoki, juniper, Mexican juniper , thorn tree, radiata pine tree, paper tree, lotus magnolia tree, baobab tree, bitter oil tree, avocado tree, camphor tree, wrinkled magnolia tree, banyan tree, catalpa, hemp walnut tree, Keppel walnut tree, black walnut Randomly select 3-5 kinds of arbor oil mixtures from walnut tree, heart-shaped walnut tree, summer walnut tree, autumn walnut tree, light walnut tree, iron walnut tree, catalpa tree, and Qinghai-Tibet plateau walnut tree to obtain embodiment 101-115.

The added amount of all arbor oil mixtures is 1.8 grams, the oil is 99.8 grams, and the oil is 1,3 propylene glycol

Table 8 Study on antioxidant performance of single arbor oil mixture and compound arbor oil mixture

(4) The application of arbor-containing tissue antioxidants in cosmetics, as shown in Table 9.

Example 116-131 In conventional cosmetic formulations, the content of alcohols is 0.5-100%, and the arbor oil mixture is 0.05%. The 110°C of the arbor oil mixture and the arbor oil mixture are tested by Swiss Metrohm oil oxidation stability tester. When the anti-oxidation stability time, wherein embodiment 126-131 is complex alcohols, used alcohols are alcohols allowed to be used in cosmetics, the compounding ratio is randomly distributed and the simple oil content is 40 grams, and the compound oil is 100 grams:

Table 9, Application of Arbor Tissue Antioxidants in Cosmetics

The above descriptions of the embodiments are for those of ordinary skill in the art to understand and use the invention. It is obvious that those skilled in the art can easily make various modifications to these embodiments, and apply the general principles described here to other embodiments without creative efforts. Therefore, the present invention is not limited to the above-mentioned embodiments. Improvements and modifications made by those skilled in the art according to the disclosure of the present invention without departing from the scope of the present invention should fall within the protection scope of the present invention.

Claims (10)

1. A method for preparing an antioxidant from arbor tissues by adopting an alcohol soaking method is characterized by comprising the following steps:

taking crushed arbor tissue powder as a raw material;

adding arbor tissue powder into a soaking tank, injecting alcohol and a permeation aid, and soaking;

extracting the alcohol-soaked arbor tissue powder with supercritical or subcritical conditions at 30-75 deg.C and 2-300atm pressure with carbon dioxide as extractant;

after supercritical or subcritical extraction, the component containing the arbor tissue extract, the alcohol and the extractant is defined as an arbor tissue antioxidant mixture, the arbor tissue antioxidant mixture is separated and purified to obtain a pure arbor tissue extract or arbor oil, and the purified arbor tissue extract or arbor oil is defined as an arbor oil mixture.

2. The method of claim 1, wherein the powder is obtained by removing impurities from arbor tissue, pulverizing the arbor tissue with a pulverizer, and sieving, and the arbor tissue is one or more selected from seeds, leaves, resin, bark, and roots.

3. The method of claim 1, wherein the arbor tissue is selected from walnut, sweet almond, american hickory, high-bush walnut, black walnut, hard-shelled walnut, ginkgo, hawthorn, magnolia, white birch, pine, red pine, snow pine, five-leaved pine, arhat pine, masson pine, red pine, elytrigia, quassia, pine, oil pine, longleaf pine, juniper, cypress, ohypnia, echinacea, japanese hinoki, american cypress, mexican cypress, acanthopanax quinans, radiata pine, broussonetia papyrifera, magnolia lilii, bread, quassima indicum, papayana, black walnut, summer walnut, and walnut.

4. The method for preparing antioxidant from arbor tissue by alcohol soaking method as claimed in claim 1, wherein the alcohol is selected from one or more of ethanol, propylene glycol, glycerol, 1,3 propylene glycol, 1,3 butylene glycol, 2,3 butylene glycol, 1,4 butylene glycol, carbitol, benzyl alcohol and phenethyl alcohol.

5. The method for preparing antioxidant from arbor tissue by alcohol soaking according to claim 1, wherein the permeation-promoting agent is selected from tocopherol, dimethylsulfone, boswellia carterii extract, paeonol, and food-grade chemically synthesized antioxidant.

6. The method for preparing antioxidant from arbor tissue by alcohol soaking method according to claim 1, wherein the weight ratio of alcohol and permeation-aid agent to arbor tissue powder is 2-8, the weight ratio of alcohol and permeation-aid agent is 1-100:1.

7. the method for preparing antioxidant from arbor tissues by alcohol soaking according to claim 1, wherein the soaking time is 2-5 h and the soaking temperature is 45-65 ℃.

8. Arbor tissue antioxidant obtained with the method according to any one of claims 1 to 7.

9. The arbor tissue antioxidant of claim 8, wherein the antioxidant comprises oleic acid at a level of 5% or more, linoleic acid at a level of 30% or more, linolenic acid at a level of 50% or more, more than 13% of the eicosa unsaturated fatty acid, retinol at a level of 0.5% or more, tocopherol at a level of 0.55% or more, phytosterol at a level of 2% or more, and flavone polypeptides at a level of 5% or more.

10. The use of an arbor tissue antioxidant as defined in claim 8, comprising arbor tissue extract, alcohol and extractant, defined as arbor tissue antioxidant cocktail, which does not require separation and purification, and which is suitable for use as antioxidant directly in cosmetics and food products; or the like, or a combination thereof,

separating and purifying the arbor tissue antioxidant mixture to obtain a pure arbor tissue extract or arbor oil, wherein the purified arbor tissue extract or arbor oil is defined as an arbor oil mixture and is used for being re-dissolved in grease to be used as an antioxidant additive.