CN115144247B - Smear device for liquid-based thin-layer cell detection - Google Patents
Smear device for liquid-based thin-layer cell detection Download PDFInfo
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- CN115144247B CN115144247B CN202211070779.5A CN202211070779A CN115144247B CN 115144247 B CN115144247 B CN 115144247B CN 202211070779 A CN202211070779 A CN 202211070779A CN 115144247 B CN115144247 B CN 115144247B
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- 239000007788 liquid Substances 0.000 title claims abstract description 98
- 238000001514 detection method Methods 0.000 title claims abstract description 35
- 230000005540 biological transmission Effects 0.000 claims abstract description 11
- 230000007246 mechanism Effects 0.000 claims description 56
- XEEYBQQBJWHFJM-UHFFFAOYSA-N Iron Chemical compound [Fe] XEEYBQQBJWHFJM-UHFFFAOYSA-N 0.000 claims description 22
- 238000001179 sorption measurement Methods 0.000 claims description 12
- 238000005485 electric heating Methods 0.000 claims description 11
- 229910052742 iron Inorganic materials 0.000 claims description 11
- 230000005389 magnetism Effects 0.000 claims description 7
- 238000009413 insulation Methods 0.000 claims description 4
- 238000007689 inspection Methods 0.000 claims description 3
- 239000000696 magnetic material Substances 0.000 claims description 3
- 239000012528 membrane Substances 0.000 abstract description 10
- 238000010438 heat treatment Methods 0.000 abstract description 9
- 230000009471 action Effects 0.000 abstract description 7
- 230000005484 gravity Effects 0.000 abstract description 4
- 238000005119 centrifugation Methods 0.000 abstract description 3
- 238000004043 dyeing Methods 0.000 abstract description 3
- 230000000295 complement effect Effects 0.000 abstract description 2
- 239000012530 fluid Substances 0.000 description 14
- 238000005192 partition Methods 0.000 description 7
- 230000001105 regulatory effect Effects 0.000 description 7
- 239000011248 coating agent Substances 0.000 description 6
- 238000000576 coating method Methods 0.000 description 6
- 230000001413 cellular effect Effects 0.000 description 5
- 238000010586 diagram Methods 0.000 description 5
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 4
- 230000000694 effects Effects 0.000 description 4
- 238000001125 extrusion Methods 0.000 description 4
- 239000011521 glass Substances 0.000 description 4
- 238000009434 installation Methods 0.000 description 4
- 238000000034 method Methods 0.000 description 4
- 230000008569 process Effects 0.000 description 3
- 230000008901 benefit Effects 0.000 description 2
- 230000008859 change Effects 0.000 description 2
- 230000001276 controlling effect Effects 0.000 description 2
- 238000001035 drying Methods 0.000 description 2
- 238000004321 preservation Methods 0.000 description 2
- 238000005070 sampling Methods 0.000 description 2
- 238000004062 sedimentation Methods 0.000 description 2
- 230000002159 abnormal effect Effects 0.000 description 1
- 238000010521 absorption reaction Methods 0.000 description 1
- 230000020828 cell gliding Effects 0.000 description 1
- 239000012535 impurity Substances 0.000 description 1
- 239000004615 ingredient Substances 0.000 description 1
- 239000012212 insulator Substances 0.000 description 1
- 230000001678 irradiating effect Effects 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 238000009595 pap smear Methods 0.000 description 1
- 239000003761 preservation solution Substances 0.000 description 1
- 238000007790 scraping Methods 0.000 description 1
- 239000004065 semiconductor Substances 0.000 description 1
- 230000001502 supplementing effect Effects 0.000 description 1
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/2813—Producing thin layers of samples on a substrate, e.g. smearing, spinning-on
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/44—Sample treatment involving radiation, e.g. heat
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/2813—Producing thin layers of samples on a substrate, e.g. smearing, spinning-on
- G01N2001/2846—Cytocentrifuge method
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- Analytical Chemistry (AREA)
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- General Health & Medical Sciences (AREA)
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- Pathology (AREA)
- Centrifugal Separators (AREA)
- Sampling And Sample Adjustment (AREA)
Abstract
The invention belongs to the technical field of liquid-based thin-layer cell detection, and particularly relates to a smear device for liquid-based thin-layer cell detection. The invention can regulate and control the liquid level of the cell sap in advance through the light transmission detection, ensure to provide enough effective cells to be detected, prevent the cells to be detected from influencing the subsequent observation too much, does not need personnel to observe, can effectively avoid the coated cells from sliding down under the action of gravity through the complementary of heating and magnetic pushing and pressurizing, eliminate the possible bubbles, effectively improve the quality of the coated cells, prevent the problems of cell overlapping, background blurring, poor subsequent dyeing and the like, and simultaneously can improve the stability of the slide and the filter membrane during centrifugation.
Description
Technical Field
The invention belongs to the technical field of liquid-based thin-layer cell detection, and particularly relates to a smear device for liquid-based thin-layer cell detection.
Background
Compared with the traditional cervical smear examination by a cervical scraping smear examination, the liquid-based thin-layer cytology examination obviously improves the satisfaction degree of the sample and the detection rate of abnormal cervical cells, and the basic steps comprise: sampling by a sampling brush, rinsing and preserving the sample by a cell preservation solution, flaking liquid-based thin-layer cells, dyeing, drying, observing by a microscope and the like.
In order to improve the smear quality of liquid-based thin-layer cells, automatic smear is generally performed by a slide making machine, such as a centrifugal sedimentation type slide making machine, the cells to be detected are uniformly smeared on a slide by matching a slide making clamp with a centrifugal driving mechanism, the slide making clamp of the centrifugal sedimentation type slide making machine has two placing modes, one mode is that the slide is vertically placed, redundant liquid can flow back after the slide making machine is used, but the lower side thickness of a cell covering area is higher than the upper side thickness due to the phenomenon that the cells on the slide sink due to gravity, so that the problems of cell overlapping, background blurring, poor subsequent dyeing and the like are caused, the other horizontally placed slide making clamp cannot flow back because the redundant liquid cannot flow back, when the slide making clamp is taken out, the redundant liquid easily drives the cells to be detected to flow out of the covering area, the using effect is not good, meanwhile, when the liquid-based thin-layer cell smear is prepared, once the cell covering area on the slide has micro-dirt, or gas in the cell covering area is not timely discharged during centrifugation, the cells after the smear are easily contain bubbles, and the bubbles are not easily found, and the judgment result of a computer can be influenced, so that the judgment of a system is influenced;
on the other hand, when taking out the upside cellular fluid in the cell preservation liquid, the personnel need observe the turbidity of cellular fluid, when the cellular fluid is comparatively clear and transparent, then it is less to show cell content in the cellular fluid, generally increase the absorption volume of cellular fluid this moment, in order to ensure to have enough effectual cell smear of examining, but the precision that personnel observed is not high, and comparatively consume energy, it is not enough to easily lead to the effective cell smear of examining of follow-up coating because of personnel's subjective consciousness, influence the smear quality, for this reason, we provide a smear device for liquid-based thin layer cell inspection and solve above-mentioned problem.
Disclosure of Invention
The invention aims to solve the problems and provides a smear device for liquid-based thin-layer cell detection.
In order to achieve the purpose, the invention adopts the following technical scheme: a smear device for liquid-based thin-layer cell detection comprises a centrifugal disc unit, wherein a transparent liquid storage cylinder is arranged above the centrifugal disc unit, a partition plate frame is fixedly arranged inside the transparent liquid storage cylinder, a plurality of liquid outlet pipes are fixedly arranged at the lower end of the side wall of the transparent liquid storage cylinder, normally closed electromagnetic valves are arranged inside the liquid outlet pipes, the pipe ends of the liquid outlet pipes are fixedly communicated with transparent hollow columns, a light transmission detection mechanism is arranged on the transparent liquid storage cylinder and comprises an upper groove plate fixedly arranged on the end face of the partition plate frame, photoresistors are fixedly arranged at the bottoms of the upper groove plate, a lower groove plate is fixedly arranged at the bottom of the transparent liquid storage cylinder, a light source is fixed at the bottom of the lower groove plate, and the light source, the photoresistors, the normally closed electromagnetic valves and the normally open electromagnetic valves are all connected with a first timing switch, each transparent hollow column is internally provided with a liquid level regulating mechanism matched with the light transmission detection mechanism, the liquid level regulating mechanism comprises a piston pad which is arranged inside the transparent hollow column in a sliding manner, an iron block is fixed at the upper end of the piston pad, an upper retaining ring is fixed at the upper side inside the transparent hollow column, a first spring is fixedly arranged between the upper retaining ring and the piston pad, an adsorption electromagnet is fixedly arranged at the inner top of the transparent hollow column, a lower retaining ring is arranged at the position, below the piston pad at the same side, inside the transparent hollow column, each adsorption electromagnet is connected with a photosensitive resistor at the same side, the side wall of the transparent liquid storage cylinder is connected with a first timing switch matched with the light transmission detection mechanism and the liquid level regulating mechanism, the bottom of each transparent hollow column is fixedly communicated with a liquid discharge pipe, a normally open electromagnetic valve is arranged inside each liquid discharge pipe, and a plurality of centrifugal cylinders are placed on the centrifugal disc unit, the side wall of each centrifugal cylinder is inserted with a communicating pipe, the pipe end of each communicating pipe is communicated with a clamping plate, a filter membrane sheet and a glass slide are placed in each clamping plate, each clamping plate is connected with a centrifugal top pressure mechanism matched with the glass slide, an electric heating plate matched with the centrifugal jacking mechanism is arranged inside the clamping plate, the side wall of each centrifugal cylinder is connected with a magnetic pushing and pressurizing mechanism matched with the communicating pipe, each magnetic pushing and pressurizing mechanism comprises a cylinder fixedly inserted in the side wall of the centrifugal cylinder, the inside of the cylinder is slidably connected with an extrusion piston matched with the communicating pipe, the side wall of the extrusion piston is provided with a U-shaped rod which is slidably connected with the cylinder end of the cylinder, a third spring is fixedly arranged between the rod wall of the U-shaped rod and the cylinder end of the cylinder, a magnetic push electromagnet is fixedly arranged on the inner wall of the cylinder, and the side wall of the extrusion piston is fixedly provided with a magnetic push block, the pipe wall of the communicating pipe is fixedly inserted with a pressure pipe, and the pressure tube is internally provided with a pressure valve, the transparent liquid storage cylinder and the centrifugal disc unit are jointly connected with a power supply mechanism, the power supply mechanism comprises an insulating machine cover fixedly arranged at the bottom of the transparent liquid storage cylinder, a conducting ring is fixedly arranged on the inner wall of the insulating machine cover, an installation groove is arranged on the end surface of the centrifugal disc unit, an insulating plate is fixedly arranged inside the mounting groove, a conductive rod which is connected with the conductive ring in a sliding way is inserted into the end surface of the insulating plate, a first electrifying delay relay and a second electrifying delay relay are respectively fixed at the bottom of the mounting groove, the bottom of insulation board is connected with second time switch and third time switch respectively, centrifugal disk unit and electric plate are connected with the second time switch through first logical electric time delay relay, magnetism pushes away the electromagnetism iron and is connected with the third time switch through the second logical electric time delay relay.
Preferably, each centrifugal roof pressure mechanism all includes the fixed box body that sets up in the splint lateral wall, the inside slip of box body is equipped with the piston board, the common fixed two second springs that are equipped with between piston board and the box body, the inner wall of splint is equipped with the gasbag pad that offsets with the slide lateral wall, be equipped with two cushion jointly between the inner wall of gasbag pad and the splint, be fixed with two outlet ducts corresponding with gasbag pad position jointly between box body and the splint, the fixed grafting of lateral wall of box body has the intake pipe, and all is equipped with the check valve in intake pipe and the two outlet ducts.
Preferably, each electric heating plate is fixedly arranged on the inner wall of the clamping plate, and the electric heating plates are arranged on the inner sides of the airbag cushions on the same side.
Preferably, the upper baffle ring is a magnetic attraction ring made of a magnetic material.
Compared with the prior art, a smear device for liquid-based thin layer cell detection has the advantages that:
1. through the mutual cooperation of the centrifugal disc set, the transparent liquid storage cylinder, the partition plate frame, the drain pipe, the normally closed solenoid valve, the transparent hollow column, the printing opacity detection mechanism, the liquid level regulation and control mechanism, first timing switch and drain pipe, through arranging the many sample cell sap to be detected in the transparent liquid storage cylinder, utilize the transparency degree of printing opacity to detect the cell sap, when the transparency is higher, it is less to explain the cell, regulate and control the liquid level in the transparent hollow column to the high point this moment, make the cell liquid measure of interpolation more, ensure that the cell volume is enough to cover the smear region of slide, in order to provide enough effectual cell of waiting to detect, when the transparency is lower, explain the cell is more, then avoid smear cell too much this moment, lead to cell multilayer cover to influence follow-up observation, need not personnel's observation, high precision, effectual.
2. Through mutually supporting of the centrifugal cylinder that sets up, communicating pipe, splint, filter membrane piece and slide, can act on the slide clamp and use for cooperation centrifugal plate unit with cell centrifugal coating on the slide, through the centrifugal roof pressure mechanism that sets up, can utilize centrifugal force, increase the crowded thrust to the slide, thereby can prevent that slide and filter membrane piece from taking place the clearance or rocking between centrifugal force effect down, ensure the definition behind the liquid base thin layer cell smear.
3. Through the electric plate who sets up, can be when centrifugal smear process, through the mode of heating, the liquid of the smear position department that reduces the slide is persisted, can reduce on the one hand that liquid moves down the dropout because of the downflow coated cell that leads to of gravity, and on the other hand can make the bubble inflation through the heating to can indirectly eliminate the bubble of coated cell department.
4. Through the magnetism push pressure mechanism that sets up, can be at the in-process of heating, through the pressure that increases in the communicating tube, utilize pressure not only to make the cell of coating attached on the glass, can break the bubble through the pressurization moreover to can form the collocation effect of supplementing each other with the electric plate, the cell gliding phenomenon that can the greatly reduced coating, and can effectively eliminate the bubble that coating cell includes, do benefit to and improve subsequent observation inspection quality.
5. Through the power supply mechanism, can supply power for electric hot plate, centrifuge dish group and magnetism push away the electro-magnet, can not hinder normal centrifugal work.
Drawings
FIG. 1 is a schematic structural diagram of a smear device for liquid-based thin-layer cell detection provided by the present invention;
FIG. 2 is a schematic structural diagram of a light transmission detection mechanism of a smear device for liquid-based thin-layer cell detection provided by the invention;
FIG. 3 is a schematic structural view of a liquid level control mechanism of a smear device for liquid-based thin layer cell detection provided by the present invention;
FIG. 4 is a schematic structural diagram of a centrifugal jacking mechanism of a smear device for liquid-based thin-layer cell detection provided by the invention;
FIG. 5 is a schematic structural diagram of a magnetic pushing and pressurizing mechanism of a smear device for liquid-based thin-layer cell detection provided by the present invention;
FIG. 6 is a schematic structural diagram of a power supply mechanism of a smear device for liquid-based thin layer cell detection provided by the present invention;
FIG. 7 is a schematic top view of a transparent liquid storage cylinder of a smear device for liquid-based thin layer cell detection according to the present invention.
In the figure: 1. a centrifugal disc unit; 2. a transparent liquid storage cylinder; 3. a partition plate frame; 4. a liquid outlet pipe; 5. a normally closed solenoid valve; 6. a transparent hollow column; 7. a light transmission detection mechanism; 71. an upper groove plate; 72. a photoresistor; 73. a lower groove plate; 74. a light source; 8. a liquid level regulating mechanism; 81. a piston pad; 82. an iron block; 83. an upper baffle ring; 84. a first spring; 85. an adsorption electromagnet; 86. a lower baffle ring; 9. a first timing switch; 10. a liquid discharge pipe; 11. a normally open solenoid valve; 12. a centrifugal cylinder; 13. a communicating pipe; 14. a splint; 15. a filter membrane sheet; 16. glass slide; 17. a centrifugal jacking mechanism; 171. a box body; 172. a piston plate; 173. a second spring; 174. an airbag cushion; 175. an elastic pad; 176. an air outlet pipe; 177. an air inlet pipe; 18. an electric hot plate; 19. a magnetic pushing and pressurizing mechanism; 191. a cylinder; 192. pushing the piston; 193. a U-shaped rod; 194. a third spring; 195. a magnetic push electromagnet; 196. a magnetic push block; 197. a pressure pipe; 20. a power supply mechanism; 201. an insulator housing; 202. a conducting ring; 203. mounting grooves; 204. an insulating plate; 205. a conductive rod; 206. a first power-on delay relay; 207. a second energized time delay relay; 208. a second timing switch; 209. and a third timing switch.
Detailed Description
The following examples are for illustrative purposes only and are not intended to limit the scope of the present invention.
As shown in fig. 1-7, a smear device for liquid-based thin-layer cell detection includes a centrifugal pan set 1, a transparent liquid storage cylinder 2 is disposed above the centrifugal pan set 1, the centrifugal pan set 1 includes a centrifugal pan and a driving motor, the centrifugal pan can be driven by the driving motor to rotate, the transparent liquid storage cylinder 2 should be fixed on a rack, a partition frame 3 is fixedly disposed inside the transparent liquid storage cylinder 2, a plurality of liquid outlet pipes 4 are fixedly disposed at a lower end of a side wall of the transparent liquid storage cylinder 2, a normally closed solenoid valve 5 is disposed inside each liquid outlet pipe 4, a transparent hollow column 6 is fixedly communicated with a pipe end of each liquid outlet pipe 4, a light transmission detection mechanism 7 is disposed on the transparent liquid storage cylinder 2, the light transmission detection mechanism 7 includes an upper slot plate 71 fixedly disposed on an end face of the partition frame 3, a photo resistor 72 is fixed on each slot bottom of the upper slot plate 71, a lower slot plate 73 is fixedly disposed at a bottom of the transparent liquid storage cylinder 2, a light source 74 is fixed on a slot bottom of the lower slot plate 73, the photo resistor 72 utilizes a photo-electric effect of a semiconductor, and when an intensity of light increases and when an irradiated light increases.
A liquid level regulating mechanism 8 matched with the light transmission detection mechanism 7 is arranged in each transparent hollow column 6, the side wall of the transparent liquid storage cylinder 2 is connected with a first timing switch 9 matched with the light transmission detection mechanism 7 and the liquid level regulating mechanism 8, the liquid level regulating mechanism 8 comprises a piston pad 81 arranged in the transparent hollow column 6 in a sliding way, an iron block 82 is fixed at the upper end of the piston pad 81, an upper retaining ring 83 is fixed at the upper side in the transparent hollow column 6, and a first spring 84 is jointly fixed between the upper retaining ring 83 and the piston pad 81, an adsorption electromagnet 85 is fixedly arranged at the inner top of the transparent hollow column 6, a lower retaining ring 86 is arranged at the position, below the piston pad 81, on the same side in the transparent hollow column 6, each adsorption electromagnet 85 is connected with the photoresistor 72 on the same side, the upper retaining ring 83 is a magnetic ring made of magnetic material, the current passing through the adsorption electromagnet 85 changes along with the change of the resistance value of the photoresistor 72, the magnetic attraction force generated by the adsorption electromagnet 85 also changes along with the change, thereby controlling the position of the piston pad 81, ensuring that the cell amount is enough during smear, the resilience force of the first spring 84 is larger than the magnetic attraction force of the upper retaining ring 83 to the iron block 82, the magnetic attraction of the upper retaining ring 83 to the iron block 82 and the magnetic attraction of the adsorption electromagnet 85 on the same side can make the iron block 82 compress the first spring 84, the iron block 82 is adsorbed to move upwards to make the first spring 84 compress and store energy, after the subsequent adsorption electromagnet 85 is powered off, at this time, because the first spring 84 needs to overcome the magnetic attraction of the upper stop ring 83 to the iron block 82, the first spring 84 cannot rebound quickly, so as to achieve the purpose of slow rebound, therefore, the phenomenon that the instantaneous extrusion force of the piston pad 81 to the cell fluid is too large to cause the cell fluid jet to splash due to the rapid rebound of the first spring 84 can be avoided.
The bottom of each transparent hollow column 6 is fixedly communicated with a liquid discharge pipe 10, the interior of each liquid discharge pipe 10 is provided with a normally open electromagnetic valve 11, a plurality of centrifugal cylinders 12 are placed on the centrifugal disc unit 1, the side wall of each centrifugal cylinder 12 is inserted with a communicating pipe 13, the pipe end of each communicating pipe 13 is communicated with a clamping plate 14, a filter membrane 15 and a slide 16 are placed inside each clamping plate 14, each clamping plate 14 is connected with a centrifugal top pressure mechanism 17 matched with the slide 16, each centrifugal top pressure mechanism 17 comprises a box body 171 fixedly arranged on the side wall of the clamping plate 14, a piston plate 172 is slidably arranged inside the box body 171, two second springs 173 are fixedly arranged between the piston plate 172 and the box body 171, and the inner wall of the clamping plate 14 is provided with an air bag pad 174 abutted against the side wall of the slide 16, two elastic cushions 175 are arranged between the inner wall of the air bag cushion 174 and the clamping plate 14, two air outlet pipes 176 corresponding to the positions of the air bag cushion 174 are fixed between the box body 171 and the clamping plate 14, an air inlet pipe 177 is fixed to the side wall of the box body 171 in an inserting mode, one-way valves are arranged in the air inlet pipe 177 and the two air outlet pipes 176, under the action of centrifugal force, the piston plate 172 can push air inside the box body 171 to enter the air bag cushion 174, the air pressure is increased, the pushing force is larger along with the increase of the centrifugal force, the centrifugal stability of the slide 16 and the filter membrane 15 can be ensured, after subsequent centrifugation is stopped, under the action of the second spring 173, the piston plate 172 moves back, the air pressure disappears at the moment, and the situation that the slide 16 and the filter membrane 15 are not taken out well due to over-tight clamping can be avoided.
The inside of splint 14 is equipped with the electric plate 18 with centrifugal roof pressure mechanism 17 matched with, each electric plate 18 all is fixed to be set up in the inner wall of splint 14, and the electric plate 18 is located the inboard setting of homonymy gasbag pad 174, through the work of electric plate 18, can utilize the temperature of heating to make the ethanol in the cell sap be heated, volatilize fast (the ethanol is for being used for carrying out the principal ingredients in the cell preservation liquid preserved to the sample cell before the smear), thereby can dry the position that the cell coated on slide 16, thereby can reduce the liquid and flow down and drive the possibility that the cell that coats drops, and utilize the temperature of heating, can make the bubble that probably exists receive the thermal expansion and burst, the heating temperature of electric plate 18 does not exceed 40 ℃, in order to prevent that the damage is effectively to examine the cell.
The side wall of each centrifuge tube 12 is connected with a magnetic pushing and pressurizing mechanism 19 matched with the communicating tube 13, each magnetic pushing and pressurizing mechanism 19 comprises a cylinder 191 fixedly inserted in the side wall of the centrifuge tube 12, the inside of the cylinder 191 is connected with a pushing piston 192 matched with the communicating tube 13 in a sliding manner, the side wall of the pushing piston 192 is provided with a U-shaped rod 193 slidably connected with the cylinder end of the cylinder 191, a third spring 194 is fixedly arranged between the electrothermal rod wall of the U-shaped rod 193 and the cylinder end of the cylinder 191, the inner wall of the cylinder 191 is fixedly provided with a magnetic pushing electromagnet 195, the side wall of the pushing piston 192 is fixedly provided with a magnetic pushing block 196, the pipe wall of the communicating tube 13 is fixedly inserted with a pressure tube 197, the pressure valve is arranged inside the pressure tube 197, the magnetic pushing electromagnet 195 works to generate magnetic pushing force, the magnetic pushing block 196 can be pushed to drive the pushing piston 192 to enter the communicating tube 13 by utilizing the same polarity repulsion, so as well as to increase the pressure in the communicating tube 13, the pressure is increased, so that the coated cells can be attached to the slide 16 and can be crushed by the pressurizing plate 18 to form the complementary effect.
The transparent liquid storage cylinder 2 and the centrifugal disk unit 1 are connected with a power supply mechanism 20, the power supply mechanism 20 comprises an insulating hood 201 fixedly arranged at the bottom of the transparent liquid storage cylinder 2, a conducting ring 202 is fixedly arranged on the inner wall of the insulating hood 201, an installation groove 203 is formed in the end face of the centrifugal disk unit 1, an insulating plate 204 is fixedly arranged inside the installation groove 203, a conducting rod 205 in sliding connection with the conducting ring 202 is inserted into the end face of the insulating plate 204, a first power-on time delay relay 206 and a second power-on time delay relay 207 are respectively fixed at the bottom of the installation groove 203, a second timing switch 208 and a third timing switch 209 are respectively connected to the bottom of the insulating plate 204, the centrifugal disk unit 1 and the electric heating plate 18 are connected with the second timing switch 208 through the first power-on time delay relay 206, the magnetic thrust magnet 195 is connected with the third timing switch 209 through the second power-on time delay relay 207, the conducting rod 205 is positioned on the conducting ring 202 to slide, normal power supply work of the centrifugal disk unit 1, the electric heating plate 18 and the magnetic thrust magnet 195 can be prevented from being influenced by centrifugal rotation, the power-on time delay relay 208 and the power-on time delay relay 207, the electric heating plate can be disconnected with the centrifugal disk unit 1, and the electric delay relay 209, and the electric delay relay 207.
The operating principle of the present invention will now be described as follows: sucking the upper layer effective cells of the cell retaining fluid out through a suction pipe and other tools and guiding the upper layer effective cells into the corresponding area of the partition plate frame 3 in the transparent fluid storage cylinder 2, then starting an equipment master control switch (not shown in the figure) to enable a first timing switch 9, a first electrifying delay relay 206 and a second electrifying delay relay 207 to be electrified and operated, starting a light source 74 through the first timing switch 9, then opening a normally closed electromagnetic valve 5, closing a normally open electromagnetic valve 11, enabling the light source 74 to work to emit light, enabling the emitted light to be blocked by cell fluid, irradiating the rest light on a photoresistor 72, enabling the cell fluid in the corresponding area to enter the transparent hollow column 6 on the same side through a liquid outlet pipe 4 if the cell fluid content is less, enabling the photoresistor 72 to pass through more current, enabling a corresponding adsorption electromagnet 85 to work to generate larger magnetic attraction force, so as to adsorb a corresponding iron block 82 to drive the piston pad 81 on the same side to move upwards, enabling the cell fluid in the corresponding area to flow into a transparent hollow column 6 on the same side through a liquid outlet pipe 4, then controlling the normally closed electromagnetic valve 74 to stop working through the first timing switch 9, and enabling the normally closed electromagnetic valve 11 to flow into a centrifugal liquid outlet pipe 12 when the cell fluid storage cylinder 10;
at this time, the first electrifying delay relay 206 enables the centrifugal disc unit 1 and the electric hot plate 18 to work through the second timing switch 208, the centrifugal disc unit 1 can drive a plurality of centrifugal cylinders 12 to rotate, effective cells in cell sap in the centrifugal cylinders 12 are coated on the slide 16 through the communicating pipes 13 under the action of rotating centrifugal force, impurities can be prevented from being coated along with the cells as much as possible under the action of the filter membrane sheet 15, the piston plate 172 moves outwards along with the increase of the centrifugal force in the centrifugal process, air in the box body 171 can be extruded to enter the clamping plate 14 from the two air outlet pipes 176, the pressure of the air bag cushion 174 on the slide 16 can be increased by increasing the pressure of the air bag cushion 174, so that the slide 16 and the filter membrane sheet 15 can be prevented from moving outwards or shaking due to the overlarge centrifugal force, and the stability in the smear process can be ensured;
the electric heating plate 18 is electrified to work, so that the temperature of the slide 16 can be gradually raised to a stable state, the electric heating plate 18 stops heating after the centrifugal disc unit 1 stops rotating under the action of the second timing switch 208, liquid on the cell coating part on the slide 16 can be dried through the heated temperature, on one hand, the phenomenon that the coated cells are fallen off due to the fact that the liquid moves downwards under the action of gravity can be reduced through drying, on the other hand, bubbles possibly contained in the coated cells can be heated to expand through heating, and therefore the bubbles can be expanded and burst, and the purpose of eliminating the bubbles is achieved;
after the centrifugal disk unit 1 stops rotating, the second electrifying delay relay 207 starts to control the magnetic pushing electromagnet 195 to work through the third timing switch 209, the magnetic pushing electromagnet 195 can generate magnetic pushing force, the magnetic pushing force can push the magnetic pushing block 196 on the same side to drive the pushing piston 192 to enter the communicating pipe 13, the pressure in the communicating pipe 13 can be increased through pushing of the pushing piston 192, and by increasing the pressure, on one hand, coated cells can be attached to the slide 16, and on the other hand, bubble burst can be eliminated through pressurization;
after an equipment master control switch (not shown in the figure) is started, the first timing switch 9, the first electrifying delay relay 206 and the second electrifying delay relay 207 are electrified to work simultaneously, the timing time of the first timing switch 9 is 30 seconds, the first electrifying delay relay 206 controls the centrifugal disc unit 1 and the electric hot plate 18 to work for 2 minutes through the second timing switch 208 after being started for 1 minute, the second electrifying delay relay 207 controls the magnetic push electromagnet 195 to work for 10 seconds through the third timing switch 209 after being started for 3 minutes, and then the smearing work of a batch of liquid-based thin layer cells is completed.
The above description is only for the purpose of illustrating the preferred embodiments of the present invention and is not to be construed as limiting the invention, and any modifications, equivalents, improvements and the like that fall within the spirit and principle of the present invention are intended to be included therein.
Claims (4)
1. The utility model provides a smear device for liquid-based thin-layer cell inspection, includes centrifugal plate unit (1), its characterized in that, the top of centrifugal plate unit (1) is equipped with transparent liquid storage cylinder (2), the transparent inside fixed baffle frame (3) that is equipped with of liquid storage cylinder (2), the transparent lateral wall lower extreme that stores liquid storage cylinder (2) is fixed and is equipped with a plurality of drain pipes (4), and the inside of each drain pipe (4) all is equipped with normally closed solenoid valve (5), each the pipe end of drain pipe (4) all is fixed the intercommunication has transparent cavity post (6), be equipped with printing opacity detection mechanism (7) on transparent liquid storage cylinder (2), printing opacity detection mechanism (7) are including fixed last frid (71) that sets up in baffle frame (3) terminal surface, each tank bottom of going up frid (71) all is fixed with photo resistance (72), the bottom of transparent liquid storage cylinder (2) is fixed and is equipped with frid (73) down, the tank bottom of frid (73) is fixed with light source (74) down, light source (74), photo resistance (5) and all be equipped with the normal closed piston post (6) when all being connected with the transparent cavity post sliding control mechanism (8) and the transparent slide control mechanism (7) and the normal open the interior of transparent piston post (8) of the regulation and control mechanism (8), the upper end of the piston pad (81) is fixed with an iron block (82), the upper side of the interior of the transparent hollow column (6) is fixed with an upper baffle ring (83), a first spring (84) is fixedly arranged between the upper baffle ring (83) and the piston pad (81), the inner top of the transparent hollow column (6) is fixedly provided with an adsorption electromagnet (85), the position of the interior of the transparent hollow column (6) below the same-side piston pad (81) is provided with a lower baffle ring (86), each adsorption electromagnet (85) is connected with a same-side photoresistor (72), the side wall of the transparent liquid storage cylinder (2) is connected with a first timing switch (9) matched with a light transmission detection mechanism (7) and a liquid level regulation and control mechanism (8), each bottom of the transparent hollow column (6) is fixedly communicated with a liquid discharge pipe (10), the interior of each liquid discharge pipe (10) is provided with an electromagnetic valve (11), a plurality of normally open centrifugal cylinders (12) are placed on the centrifugal plate set (1), the side wall of each centrifugal cylinder (12) is inserted with a liquid discharge pipe (13), each liquid discharge pipe (13) is connected with a liquid filter plate (14), each slide (14) and each slide filter plate (14) is connected with a slide filter plate (14), and each slide (14) are connected with a slide filter plate (14), and the inside of splint (14) is equipped with centrifugal roof pressure mechanism (17) matched with electric plate (18), each the lateral wall of centrifuge bowl (12) all be connected with communicating pipe (13) assorted magnetism push pressurizing mechanism (19), each magnetism pushes pressurizing mechanism (19) all including fixed drum (191) of pegging graft in centrifuge bowl (12) lateral wall, the inside sliding connection of drum (191) have with communicating pipe (13) assorted crowded push piston (192), the lateral wall of crowded push piston (192) is equipped with U-shaped pole (193) that has with drum (191) barrel end sliding connection, and between the pole wall of U-shaped pole (193) and the barrel end of drum (191) jointly fixed be equipped with third spring (194), the inner wall of drum (191) is fixed and is equipped with magnetism and pushes away electro-magnet (195), and the lateral wall of crowded push piston (192) is fixed and is equipped with magnetism and pushes away piece (196), the pipe wall of communicating pipe (13) is fixed and is pegged graft and is equipped with pressure pipe (197), and the inside of pressure pipe (197) is equipped with the pressure valve, transparent deposit liquid barrel (2) and power supply dish (1) power supply unit (20) and fixed insulation mechanism (201) are connected with the fixed cover of fixed and the bottom of fixed insulation machine cover (201), the fixed insulation mechanism (201) is equipped with the transparent deposit liquid deposit machine cover (201), the end face of the centrifugal disk unit (1) is provided with a mounting groove (203), an insulating plate (204) is fixedly arranged inside the mounting groove (203), a conducting rod (205) in sliding connection with the conducting ring (202) is inserted into the end face of the insulating plate (204), a first power-on time delay relay (206) and a second power-on time delay relay (207) are fixed to the bottom of the mounting groove (203), the bottom of the insulating plate (204) is connected with a second time switch (208) and a third time switch (209) respectively, the centrifugal disk unit (1) and the electric heating plate (18) are connected with the second time switch (208) through the first power-on time delay relay (206), and the magnetic electromagnet (195) is connected with the third time switch (209) through the second power-on time delay relay (207).
2. The smear device for liquid-based thin-layer cell detection according to claim 1, wherein each of the centrifugal pressing mechanisms (17) comprises a box body (171) fixedly arranged on the side wall of the clamping plate (14), a piston plate (172) is slidably arranged inside the box body (171), two second springs (173) are fixedly arranged between the piston plate (172) and the box body (171) together, an air bag cushion (174) abutting against the side wall of the slide (16) is arranged on the inner wall of the clamping plate (14), two elastic cushions (175) are arranged between the inner wall of the air bag cushion (174) and the clamping plate (14) together, two air outlet pipes (176) corresponding to the positions of the air bag cushion (174) are fixed between the box body (171) and the clamping plate (14) together, an air inlet pipe (177) is fixedly inserted into the side wall of the box body (171), and one-way valves are arranged in the air inlet pipe (177) and the two air outlet pipes (176).
3. The smear device for LC-based thin layer cell detection as claimed in claim 2, wherein each of the electric heating plates (18) is fixedly arranged on the inner wall of the splint (14), and the electric heating plates (18) are arranged on the inner side of the airbag cushion (174) on the same side.
4. The smear device for LC-TLC detection according to claim 1, wherein the upper baffle ring (83) is a magnetic ring made of magnetic material.
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| CN116125053B (en) * | 2023-02-21 | 2024-05-31 | 天津美士邦涂料化工有限公司 | Test equipment for detecting phenolic epoxy resin |
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