CN112857941A - Cell smear dyeing device and using method thereof - Google Patents
Cell smear dyeing device and using method thereof Download PDFInfo
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- CN112857941A CN112857941A CN202110208762.0A CN202110208762A CN112857941A CN 112857941 A CN112857941 A CN 112857941A CN 202110208762 A CN202110208762 A CN 202110208762A CN 112857941 A CN112857941 A CN 112857941A
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/2813—Producing thin layers of samples on a substrate, e.g. smearing, spinning-on
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/30—Staining; Impregnating ; Fixation; Dehydration; Multistep processes for preparing samples of tissue, cell or nucleic acid material and the like for analysis
- G01N1/31—Apparatus therefor
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/44—Sample treatment involving radiation, e.g. heat
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/30—Staining; Impregnating ; Fixation; Dehydration; Multistep processes for preparing samples of tissue, cell or nucleic acid material and the like for analysis
- G01N1/31—Apparatus therefor
- G01N2001/317—Apparatus therefor spraying liquids onto surfaces
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Abstract
本发明涉及一种细胞涂片染色装置及其使用方法，包括多个盛液箱、多个电磁阀、多个喷液头、转换盘、柜体、玻片盒、旋转机构和操作面板；多个盛液箱可拆卸地支撑在柜体上侧，多个盛液箱与多个电磁阀一一对应，且每个电磁阀与对应的盛液箱连接，多个电磁阀与多个喷液头一一对应，且每个喷液头与对应的电磁阀连接，多个喷液头还与转换盘连接，且每个喷液头沿圆周排列，转换盘可旋转地连接在柜体上；玻片盒可旋转地连接在柜体下侧，玻片盒呈圆盘状，且玻片盒包括多个独立盒，多个独立盒沿圆周方向排列，每个独立盒中用于盛放玻片。本发明用于细胞自动染色，能够实现每个玻片的独立染色，在玻片盒往复晃动时，能够使玻片上的染液扩散更加均匀。
The invention relates to a cell smear staining device and a method for using the same. Each liquid storage tank is detachably supported on the upper side of the cabinet, the multiple liquid storage tanks are in one-to-one correspondence with the multiple solenoid valves, and each solenoid valve is connected to the corresponding liquid storage tank, and the multiple solenoid valves are connected to the multiple liquid spraying tanks. The heads are in one-to-one correspondence, and each liquid ejection head is connected with the corresponding solenoid valve, and a plurality of liquid ejection heads are also connected with the switching disk, and each liquid ejecting head is arranged along the circumference, and the switching disk is rotatably connected to the cabinet; The slide box is rotatably connected to the lower side of the cabinet, the slide box is in the shape of a disc, and the slide box includes a plurality of independent boxes, and the plurality of independent boxes are arranged in the circumferential direction, and each independent box is used for holding glass slides. piece. The invention is used for automatic staining of cells, can realize independent staining of each glass slide, and can make the dye solution on the glass slide spread more evenly when the slide box is shaken back and forth.
Description
The technical field is as follows:
the invention relates to the technical field of biological experimental equipment, in particular to a cell smear dyeing device and a using method thereof.
Background art:
in medical testing, experiments in microbiology, cell biology, etc. are often performed to facilitate clinical pathogen diagnosis. Prior to microscopic examination, the cytological specimen is smeared onto a slide and a stain is added dropwise to the slide. In order to perform cell staining efficiently, a cell smear staining apparatus is required. In the patent with the patent number zl201811526748.x, a reaction box is formed by combining a reaction box body and an internal filling block, the reaction box body is rectangular, heating grooves are respectively arranged on the outer wall surfaces of the peripheral walls of the reaction box body, and heating sheets are arranged in the heating grooves; a reagent injection port is arranged at the corner of the bottom of the reaction box body; the bottom of the reaction box body is inclined, and a liquid level detection groove is arranged on the side wall corresponding to the bottom of the box body adjacent to the reagent injection port; a liquid inlet is arranged on the side wall opposite to the side wall provided with the liquid level detection groove. When the reagent box is used, a plurality of slides are placed in the reaction box body, and when the liquid level of the reagent reaches a set position, all the slides can be submerged by the reagent. However, the structure can only uniformly dye the glass slides in batch, cannot realize independent dyeing of a plurality of glass slides, is not easy to uniformly dye cells on the glass slides, and cannot meet the use requirement.
The invention content is as follows:
technical problem to be solved
The invention aims to provide a cell smear dyeing device and a using method thereof, which are used for automatically dyeing cells and solve the problems that the conventional cell smear dyeing device cannot realize independent dyeing of a plurality of slides and is not easy to uniformly dye the cells on the slides.
(II) technical scheme
In order to solve the technical problems, the invention adopts the following technical scheme: a cell smear dyeing device comprises a plurality of liquid containing boxes, a plurality of electromagnetic valves, a plurality of liquid spraying heads, a conversion disc, a cabinet body, a slide box, a rotating mechanism and an operation panel;
the plurality of liquid containing boxes are detachably supported on the upper side of the cabinet body, the plurality of liquid containing boxes correspond to the plurality of electromagnetic valves one to one, each electromagnetic valve is connected with the corresponding liquid containing box, the plurality of electromagnetic valves correspond to the plurality of liquid spraying heads one to one, each liquid spraying head is connected with the corresponding electromagnetic valve, the plurality of liquid spraying heads are further connected with the conversion disc, each liquid spraying head is arranged along the circumference, and the conversion disc is rotatably connected to the cabinet body;
the slide box is rotatably connected to the lower side of the cabinet body through the rotating mechanism, is disc-shaped and comprises a plurality of independent boxes which are arranged along the circumferential direction, each independent box is used for containing a slide, and the slide box can also realize reciprocating small-amplitude shaking in the horizontal plane through controlling rotation;
the operation panel is used for controlling the opening and closing of the electromagnetic valves, the rotation of the switching disc and the rotation and shaking of the slide box.
Through the arrangement of the liquid containing boxes, the electromagnetic valves and the liquid spraying heads which are in one-to-one correspondence, liquid in the liquid containing boxes can flow to the liquid spraying heads under the control of the electromagnetic valves, and then the liquid spraying heads can spray liquid to the slide boxes arranged on the lower side of the cabinet body. Since the plurality of liquid ejecting heads are circumferentially connected to the switching plate, the switching plate can switch the different liquid ejecting heads to eject liquid onto the slide cassette after rotating. The liquid containing box can contain dye liquids with different colors, so that the dip dyeing of different colors can be performed on a plurality of slides. The slides in the independent boxes are not interfered with each other, the independent dyeing of the slides can be carried out, after the slide box rotates, other independent boxes can be switched to be positioned below the liquid spraying heads, so that the independent dyeing of each slide is further realized, and the mixed dyeing of the slides can be realized by spraying different dyeing liquids to the slide box by a plurality of different liquid spraying heads through the rotation of the conversion disc; when the slide box shakes to and fro, the dye solution on the slide can be diffused more uniformly.
Further, slide box still includes the slide frame, slide frame fixed connection be in the inner wall of independent box, the slide frame has recess and guiding gutter, the size of recess is greater than the size of slide, the recess is used for holding the slide, the guiding gutter is located the lateral wall of recess, slide can place in the recess of slide frame, the degree of depth that recess self has can prevent that the slide from falling out, the guiding gutter can with the liquid discharge in the recess extremely in the independent box.
Further, the slide frame still has a plurality of separation bosss, and is a plurality of separate the boss and be the strip structure, and is a plurality of separate two liang of parallel distribution of boss in the tank bottom of recess, two separate between the boss to and separate the boss with the slide can all be held between the inner wall of recess, can with a plurality of slides tile in on the slide frame, can realize the batch dyeing of slide, separate the boss and can keep apart between per two slides, prevent the random drunkenness between two slides.
Further, the slide box further comprises a heating pad, the heating pad is located below the independent boxes, and the heating pad can heat the slide to enable dye liquid on the slide to be dried quickly.
Further, cell smear dyeing apparatus still includes pipe and fluid-discharge tube, the one end of pipe with the solenoid valve is connected, the other end of pipe with hydrojet head is connected, the pipe can with flowing through the dye liquor of solenoid valve carry to hydrojet head, the fluid-discharge tube includes a plurality of liquid sections of adopting and flowing back section, and is a plurality of the liquid section of adopting is even independent box is connected, every liquid section of adopting all with the flowing back section intercommunication, the fluid-discharge tube can with remaining liquid in the independent box discharges independent box.
Furthermore, valves are respectively arranged on a plurality of liquid collecting sections of the liquid discharge pipe, and liquid in the independent boxes can be discharged outwards after the valves are opened.
Further, the rotating mechanism comprises a motor, a gear assembly, an inner gear ring and a rotating disk, the motor is fixedly connected to the lower side of the cabinet body, the motor is further connected with the gear assembly, the gear assembly is in transmission connection with the inner gear ring, the rotating disk is of a hollow disc-shaped structure, the outer ring of the inner gear ring is fixedly connected with the inner ring of the rotating disk, the glass box is arranged on the rotating disk, the motor drives the gear assembly to rotate, and the gear assembly drives the inner gear ring to rotate so as to drive the rotating disk to rotate.
Further, including at least one flourishing liquid case in a plurality of flourishing liquid casees and being used for holding the dye liquor, be used for holding the clear water including at least one flourishing liquid case, not only can carry out the cell staining, can also wash the residual dye liquor on the slide.
Further, flourishing liquid box is the echelonment, be provided with the support frame on the cabinet body, the support frame is based on flourishing liquid box's ladder department supports flourishing liquid box, the flourishing liquid box of echelonment is convenient for support, and can high-efficient the dismantlement.
The invention also provides a using method of the cell smear dyeing device, which comprises the following steps:
step one, dye solutions and clear water with different colors are poured into the liquid containing boxes respectively, and one or more slides loaded with cells are independently placed on the slide racks in the independent boxes;
rotating the conversion disc through the operation panel to enable a liquid spraying head corresponding to the target dye liquor to face a vertically downward direction;
rotating the slide box through the operation panel to enable the independent box containing the slide to be dyed to be positioned right below the liquid spraying head corresponding to the target dyeing liquid;
fourthly, controlling an electromagnetic valve corresponding to the target dye solution to be opened through the operation panel, enabling a corresponding liquid spraying head to spray the dye solution to the slide to be dyed, simultaneously controlling the slide box to reciprocate and shake in a small amplitude, enabling the dye solution to be fully contacted with cells on the slide, and then controlling the electromagnetic valve to be closed;
step five, the heating pad is controlled to generate heat through the operation panel, so that the slide is dried quickly, the valve is opened, the redundant dye liquor in the independent box flows away, the conversion disc is rotated through the operation panel, the electromagnetic valve corresponding to the clear water is opened, and the clear water is sprayed to the slide through the liquid spraying head to clean the residual dye liquor on the slide.
(III) advantageous effects
Compared with the prior art, the invention has the following beneficial effects: through setting up the flourishing liquid case, solenoid valve and the hydrojet of one-to-one, the liquid in the flourishing liquid case can flow to the hydrojet under the control of solenoid valve, and then the hydrojet sprays liquid to the slide box that sets up in cabinet body downside, and a plurality of hydrojet are connected on the change-over disc along the circumference, can switch over different hydrojet heads and spray liquid to the slide box. The slide mutual noninterference in a plurality of independent boxes can carry out the independent dyeing of slide, and after slide box is rotatory, can switch other independent boxes and be located the hydrojet head below, and then realize the independent dyeing of every slide to when slide box can reciprocate rock, the dye liquor diffusion on the messenger slide is more even.
Description of the drawings:
in order to more clearly illustrate the technical solution in the embodiments of the present invention, the drawings required to be used in the embodiments will be briefly described below.
FIG. 1 is a schematic structural diagram of a cell smear staining apparatus according to an embodiment of the present invention;
fig. 2 is a schematic top view of the rotating mechanism according to the embodiment of the present invention;
fig. 3 is a schematic cross-sectional view of a configuration of a cassette according to an embodiment of the present invention;
fig. 4 is a schematic cross-sectional view of another alternative cassette configuration in accordance with an embodiment of the present invention;
FIG. 5 is a schematic structural diagram of a valve according to an embodiment of the present invention;
FIG. 6 is a schematic structural diagram of a support frame according to an embodiment of the present invention;
in the figure: 1. a liquid containing box; 2. an electromagnetic valve; 3. a liquid jet head; 4. a switching disk; 5. a cabinet body; 6. a cassette for glass sheets; 7. a rotation mechanism; 8. an operation panel; 9. a conduit; 10. a liquid discharge pipe; 51. a support frame; 61. a separate cassette; 62. a slide rack; 63. a groove; 64. a diversion trench; 65. separating the bosses; 66. a heating pad; 67. glass slide; 71. a motor; 72. a gear assembly; 73. an inner gear ring; 74. rotating the disc; 101. valve gate
The specific implementation mode is as follows:
the technical solution in the embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings in the embodiments of the present invention.
As shown in fig. 1, fig. 2, fig. 3, fig. 4, fig. 5 and fig. 6, a cell smear staining apparatus includes a plurality of liquid containers 1, a plurality of solenoid valves 2, a plurality of liquid-jet heads 3, a switching plate 4, a cabinet 5, a slide cassette 6, a rotating mechanism 7 and an operation panel 8;
the liquid containing boxes 1 are detachably supported on the upper side of the cabinet body 5, the liquid containing boxes 1 correspond to the electromagnetic valves 2 one by one, each electromagnetic valve 2 is connected with the corresponding liquid containing box 1, the electromagnetic valves 2 correspond to the liquid spraying heads 3 one by one, each liquid spraying head 3 is connected with the corresponding electromagnetic valve 2, the liquid spraying heads 3 are further connected with the conversion disc 4, the liquid spraying heads 3 are arranged along the circumference, and the conversion disc 4 is rotatably connected to the cabinet body 5;
the slide box 6 is rotatably connected to the lower side of the cabinet body 5 through a rotating mechanism 7, the slide box 6 is disc-shaped, the slide box 6 comprises a plurality of independent boxes 61, the independent boxes 61 are arranged along the circumferential direction, each independent box 61 is used for containing slides, and the slide box 6 can also shake in a horizontal plane;
the operation panel 8 is used to control the opening and closing of the plurality of electromagnetic valves 2, as well as the rotation of the switching disk 4, the rotation and shaking of the cassette 6.
It should be noted that the liquid in the independent box 61 does not interfere with the liquid in the other independent boxes 61, and each slide can be independently stained without interference. When the disk-shaped slide cassette 6 is rotated to switch the other individual cassettes 61, no other space is occupied.
Preferably, the slide cassette 6 further includes a slide rack 62, the slide rack 62 is fixedly connected to the inner wall of the independent cassette 61, the slide rack 62 has a groove 63 and a guiding groove 64, the size of the groove 63 is larger than that of the slide, the groove 63 is used for holding the slide 67, the guiding groove 64 is located on the side wall of the groove 63, thus, the slide 67 can be placed in the groove 63 of the slide rack 62, the depth of the groove 63 itself can prevent the slide 67 from falling out, and the guiding groove 64 can discharge the liquid in the groove 63 to the independent cassette 61. .
Preferably, the slide frame 62 further has a plurality of separating bosses 65, the separating bosses 65 are in a strip structure, and the separating bosses 65 are distributed on the bottom of the groove 63 in pairs in parallel, and the slide 67 can be accommodated between the two separating bosses 65 and between the separating bosses 65 and the inner wall of the groove 63. Thus, a plurality of slides 67 can be laid on the slide rack 62, batch dyeing of the slides 67 can be realized, and the partition bosses 65 can separate every two slides 67 to prevent random movement between the two slides 67.
Preferably, the cassette 6 further comprises a heating mat 66, the heating mat 66 being located below the plurality of individual cassettes 61. The heating pad 66 can heat the slide to dry the stain quickly on the slide.
Preferably, the cell smear dyeing apparatus further comprises a conduit 9 and a liquid discharge pipe 10, one end of the conduit 9 is connected with the electromagnetic valve 2, and the other end of the conduit 9 is connected with the liquid spray head 3. The pipe 9 can be carried the dye liquor that flows through the solenoid valve 2 to hydrojet head 3, and fluid-discharge tube 10 includes a plurality of liquid sections of adopting and flowing back section, and a plurality of liquid sections of adopting are even independent box 61 and are connected, and every liquid section of adopting all communicates with the flowing back section. The drain pipe 10 can discharge the remaining liquid in the individual cartridge 61 out of the individual cartridge 61.
Preferably, valves 101 are respectively arranged on a plurality of liquid production sections of the liquid discharge pipe, and the liquid in the independent box can be discharged outwards after the valves 101 are opened.
Preferably, the rotating mechanism 7 comprises a motor 71, a gear assembly 72, an inner gear ring 73 and a rotating disk 74, the motor 71 is fixedly connected to the lower side of the cabinet 5, the motor 71 is further connected with the gear assembly 72, the gear assembly 72 is in transmission connection with the inner gear ring 73, the rotating disk 74 is a hollow disc-shaped structure, the outer ring of the inner gear ring 73 is fixedly connected with the inner ring of the rotating disk 74, and the slide cassette 6 is arranged on the rotating disk 74. Wherein, the slide rail can be set up below the rotary disk 74, and the rotary disk 74 passes through the slide rail and cabinet 5 sliding connection, perhaps sets up the gyro wheel below the rotary disk 74, and then can with cabinet 5 rolling connection. The motor 71 drives the gear assembly 72 to rotate, the gear assembly 72 drives the inner gear ring 73 to rotate, and further drives the rotating disc 74 to rotate, and when the motor 71 is controlled to rotate positively and negatively, the slide box 6 can be controlled to rotate in a reciprocating manner, so that the effect of reciprocating shaking is achieved.
Preferably, the plurality of liquid containing boxes 1 comprise at least one liquid containing box 1 for containing dye liquid and at least one liquid containing box 1 for containing clean water. Thus, not only cell staining but also washing of residual staining solution on the slide can be performed.
Preferably, the liquid containing box 1 is stepped, the cabinet body 5 is provided with a support frame 51, and the support frame 51 supports the liquid containing box 1 based on the step of the liquid containing box 1. Like this, the flourishing liquid case 1 of echelonment is convenient for support, and can high-efficient dismantlement.
The application method of the cell smear staining device provided by the embodiment of the invention comprises the following steps:
when in use, dye solutions and clear water with different colors are respectively poured into the liquid containing boxes 1, and one or more slides loaded with cells are independently placed on the slide racks 62 in the independent boxes 61; of course, slides may also be placed on the slide racks 62 by an automatic feed mechanism; rotating the conversion disk 4 through the operation panel 8 so that the liquid ejecting head 3 corresponding to the target dye liquid is directed in a vertically downward direction; the slide cassette 6 is rotated by the operation panel 8 so that the independent cassette 61 containing the slide to be stained is positioned right below the liquid ejecting head 3 corresponding to the target staining solution; the electromagnetic valve 2 corresponding to the target dye solution is controlled to be opened through the operation panel 8, so that the corresponding liquid spraying head 3 sprays the dye solution to the slide to be dyed, meanwhile, the slide box 6 is controlled to reciprocate and shake in a small range, so that the dye solution is fully contacted with cells on the slide, and the electromagnetic valve 2 is controlled to be closed when the dye solution is sprayed to 1 ml; heating pad 66 is controlled through operation panel 8 to generate heat, so that the slide is dried fast, valve 101 is opened, redundant dye liquor in independent box 61 flows away, conversion disc 4 is rotated through operation panel 8, solenoid valve 2 corresponding to clear water is opened, and clear water is sprayed to the slide through liquid spraying head 3 to clean residual dye liquor on the slide. And finally, taking away the slide by an automatic feeding mechanism. Of course, after the slide is sufficiently impregnated with the dye solution for 2 minutes, the conversion disk 4 can be controlled to rotate, the liquid spraying head 3 corresponding to the dye solution of the other color is rotated to be vertically downward, namely to face the slide which is just dyed, the dye solution of the other color is further sprayed to the slide, and meanwhile, the slide box 6 is shaken back and forth, so that the dye solutions of the two colors are uniformly mixed.
The liquid in the independent box 61 does not interfere with the liquid in other independent boxes 61, so that independent dyeing is realized without interference.
In summary, the cell smear staining device and the use method thereof provided by the invention are used for automatically staining cells, and solve the problems that the existing cell smear staining device cannot realize independent staining of a plurality of slides and is not easy to stain the cells on the slides uniformly.
The present invention has been described above by way of example, but the present invention is not limited to the above-described specific embodiments, and any modification or variation made based on the present invention is within the scope of the present invention as claimed.
Claims (10)
1. The utility model provides a cell smear dyeing apparatus which characterized in that: comprises a plurality of liquid containing boxes, a plurality of electromagnetic valves, a plurality of liquid spraying heads, a conversion disc, a cabinet body, a glass sheet box, a rotating mechanism and an operation panel;
the plurality of liquid containing boxes are detachably supported on the upper side of the cabinet body, the plurality of liquid containing boxes correspond to the plurality of electromagnetic valves one to one, each electromagnetic valve is connected with the corresponding liquid containing box, the plurality of electromagnetic valves correspond to the plurality of liquid spraying heads one to one, each liquid spraying head is connected with the corresponding electromagnetic valve, the plurality of liquid spraying heads are further connected with the conversion disc, each liquid spraying head is arranged along the circumference, and the conversion disc is rotatably connected to the cabinet body;
the slide box is rotatably connected to the lower side of the cabinet body through the rotating mechanism, is disc-shaped and comprises a plurality of independent boxes which are arranged along the circumferential direction, each independent box is used for containing a slide, and the slide box can also shake in a horizontal plane;
the operation panel is used for controlling the opening and closing of the electromagnetic valves, the rotation of the switching disc and the rotation and shaking of the slide box.
2. A cell smear staining apparatus according to claim 1 wherein: the slide box further comprises a slide frame, the slide frame is fixedly connected to the inner wall of the independent box, the slide frame is provided with a groove and a flow guide groove, the size of the groove is larger than that of a slide, the groove is used for containing the slide, and the flow guide groove is located on the side wall of the groove.
3. A cell smear staining apparatus according to claim 2 wherein: the slide frame still has a plurality of separating boss, and is a plurality of separate the boss and be the strip structure, and is a plurality of two liang of parallel distribution in separate the boss in the tank bottom of recess, two separate between the boss to and separate the boss with all can hold the slide between the inner wall of recess.
4. A cell smear staining apparatus according to claim 1 wherein: the cassette further includes a heating pad positioned below the plurality of individual cassettes.
5. A cell smear staining apparatus according to claim 1 wherein: the cell smear dyeing device further comprises a guide pipe and a liquid discharge pipe, one end of the guide pipe is connected with the electromagnetic valve, the other end of the guide pipe is connected with the liquid spraying head, the liquid discharge pipe comprises a plurality of liquid collecting sections and liquid discharge sections, the liquid collecting sections are uniform, the independent boxes are connected, and each liquid collecting section is communicated with the liquid discharge section.
6. A cell smear staining apparatus according to claim 5 wherein: and valves are respectively arranged on the liquid collecting sections of the liquid discharge pipe.
7. A cell smear staining apparatus according to claim 1 wherein: the rotating mechanism comprises a motor, a gear assembly, an inner gear ring and a rotating disk, the motor is fixedly connected to the lower side of the cabinet body, the motor is further connected with the gear assembly, the gear assembly is in transmission connection with the inner gear ring, the rotating disk is of a hollow disc-shaped structure, the outer ring of the inner gear ring is fixedly connected with the inner ring of the rotating disk, and the glass box is arranged on the rotating disk.
8. A cell smear staining apparatus according to claim 1 wherein: the plurality of liquid containing boxes comprise at least one liquid containing box for containing dye liquid and at least one liquid containing box for containing clear water.
9. A cell smear staining apparatus according to claim 1 wherein: the liquid containing box is in a step shape, a support frame is arranged on the cabinet body, and the support frame supports the liquid containing box based on the step of the liquid containing box.
10. The use method of the cell smear staining apparatus according to any one of claims 1 to 9, comprising the steps of:
step one, dye solutions and clear water with different colors are poured into the liquid containing boxes respectively, and one or more slides loaded with cells are independently placed on the slide racks in the independent boxes;
rotating the conversion disc through the operation panel to enable a liquid spraying head corresponding to the target dye liquor to face a vertically downward direction;
rotating the slide box through the operation panel to enable the independent box containing the slide to be dyed to be positioned right below the liquid spraying head corresponding to the target dyeing liquid;
fourthly, controlling an electromagnetic valve corresponding to the target dye solution to be opened through the operation panel, enabling a corresponding liquid spraying head to spray the dye solution to the slide to be dyed, simultaneously controlling the slide box to reciprocate and shake in a small amplitude, enabling the dye solution to be fully contacted with cells on the slide, and then controlling the electromagnetic valve to be closed;
step five, the heating pad is controlled to generate heat through the operation panel, so that the slide is dried quickly, the valve is opened, the redundant dye liquor in the independent box flows away, the conversion disc is rotated through the operation panel, the electromagnetic valve corresponding to the clear water is opened, and the clear water is sprayed to the slide through the liquid spraying head to clean the residual dye liquor on the slide.
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| CN202110208762.0A CN112857941A (en) | 2021-02-24 | 2021-02-24 | Cell smear dyeing device and using method thereof |
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| CN202110208762.0A CN112857941A (en) | 2021-02-24 | 2021-02-24 | Cell smear dyeing device and using method thereof |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115144247A (en) * | 2022-09-02 | 2022-10-04 | 深圳明灏生物科技有限公司 | Smear device for liquid-based thin-layer cell detection |
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