CN115119841A - Novel chitosan composite cationic disinfectant suitable for high-power dilution - Google Patents
Novel chitosan composite cationic disinfectant suitable for high-power dilution Download PDFInfo
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- CN115119841A CN115119841A CN202210909187.1A CN202210909187A CN115119841A CN 115119841 A CN115119841 A CN 115119841A CN 202210909187 A CN202210909187 A CN 202210909187A CN 115119841 A CN115119841 A CN 115119841A
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- disinfectant
- chitosan
- amino acid
- water
- solution
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- 239000000645 desinfectant Substances 0.000 title claims abstract description 72
- 229920001661 Chitosan Polymers 0.000 title claims abstract description 53
- 238000010790 dilution Methods 0.000 title claims abstract description 27
- 239000012895 dilution Substances 0.000 title claims abstract description 27
- 125000002091 cationic group Chemical group 0.000 title claims abstract description 26
- 239000002131 composite material Substances 0.000 title claims description 12
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 34
- 235000001014 amino acid Nutrition 0.000 claims abstract description 27
- -1 compound amino acid Chemical class 0.000 claims abstract description 25
- 150000001875 compounds Chemical class 0.000 claims abstract description 14
- 239000002994 raw material Substances 0.000 claims abstract description 12
- 239000003093 cationic surfactant Substances 0.000 claims abstract description 7
- DHMQDGOQFOQNFH-UHFFFAOYSA-N Glycine Chemical compound NCC(O)=O DHMQDGOQFOQNFH-UHFFFAOYSA-N 0.000 claims abstract description 6
- 239000004475 Arginine Substances 0.000 claims abstract description 3
- WHUUTDBJXJRKMK-UHFFFAOYSA-N Glutamic acid Natural products OC(=O)C(N)CCC(O)=O WHUUTDBJXJRKMK-UHFFFAOYSA-N 0.000 claims abstract description 3
- 239000004471 Glycine Substances 0.000 claims abstract description 3
- PMMYEEVYMWASQN-DMTCNVIQSA-N Hydroxyproline Chemical compound O[C@H]1CN[C@H](C(O)=O)C1 PMMYEEVYMWASQN-DMTCNVIQSA-N 0.000 claims abstract description 3
- ONIBWKKTOPOVIA-BYPYZUCNSA-N L-Proline Chemical compound OC(=O)[C@@H]1CCCN1 ONIBWKKTOPOVIA-BYPYZUCNSA-N 0.000 claims abstract description 3
- QNAYBMKLOCPYGJ-REOHCLBHSA-N L-alanine Chemical compound C[C@H](N)C(O)=O QNAYBMKLOCPYGJ-REOHCLBHSA-N 0.000 claims abstract description 3
- ODKSFYDXXFIFQN-BYPYZUCNSA-P L-argininium(2+) Chemical compound NC(=[NH2+])NCCC[C@H]([NH3+])C(O)=O ODKSFYDXXFIFQN-BYPYZUCNSA-P 0.000 claims abstract description 3
- CKLJMWTZIZZHCS-REOHCLBHSA-N L-aspartic acid Chemical compound OC(=O)[C@@H](N)CC(O)=O CKLJMWTZIZZHCS-REOHCLBHSA-N 0.000 claims abstract description 3
- WHUUTDBJXJRKMK-VKHMYHEASA-N L-glutamic acid Chemical compound OC(=O)[C@@H](N)CCC(O)=O WHUUTDBJXJRKMK-VKHMYHEASA-N 0.000 claims abstract description 3
- ONIBWKKTOPOVIA-UHFFFAOYSA-N Proline Natural products OC(=O)C1CCCN1 ONIBWKKTOPOVIA-UHFFFAOYSA-N 0.000 claims abstract description 3
- 235000004279 alanine Nutrition 0.000 claims abstract description 3
- 235000009697 arginine Nutrition 0.000 claims abstract description 3
- ODKSFYDXXFIFQN-UHFFFAOYSA-N arginine Natural products OC(=O)C(N)CCCNC(N)=N ODKSFYDXXFIFQN-UHFFFAOYSA-N 0.000 claims abstract description 3
- 235000003704 aspartic acid Nutrition 0.000 claims abstract description 3
- OQFSQFPPLPISGP-UHFFFAOYSA-N beta-carboxyaspartic acid Natural products OC(=O)C(N)C(C(O)=O)C(O)=O OQFSQFPPLPISGP-UHFFFAOYSA-N 0.000 claims abstract description 3
- PMMYEEVYMWASQN-UHFFFAOYSA-N dl-hydroxyproline Natural products OC1C[NH2+]C(C([O-])=O)C1 PMMYEEVYMWASQN-UHFFFAOYSA-N 0.000 claims abstract description 3
- 235000013922 glutamic acid Nutrition 0.000 claims abstract description 3
- 239000004220 glutamic acid Substances 0.000 claims abstract description 3
- 229960002591 hydroxyproline Drugs 0.000 claims abstract description 3
- FGMPLJWBKKVCDB-UHFFFAOYSA-N trans-L-hydroxy-proline Natural products ON1CCCC1C(O)=O FGMPLJWBKKVCDB-UHFFFAOYSA-N 0.000 claims abstract description 3
- 239000000243 solution Substances 0.000 claims description 32
- 238000003756 stirring Methods 0.000 claims description 26
- 229920002413 Polyhexanide Polymers 0.000 claims description 19
- 229960002233 benzalkonium bromide Drugs 0.000 claims description 19
- KHSLHYAUZSPBIU-UHFFFAOYSA-M benzododecinium bromide Chemical compound [Br-].CCCCCCCCCCCC[N+](C)(C)CC1=CC=CC=C1 KHSLHYAUZSPBIU-UHFFFAOYSA-M 0.000 claims description 19
- RUPBZQFQVRMKDG-UHFFFAOYSA-M Didecyldimethylammonium chloride Chemical compound [Cl-].CCCCCCCCCC[N+](C)(C)CCCCCCCCCC RUPBZQFQVRMKDG-UHFFFAOYSA-M 0.000 claims description 17
- 229960004670 didecyldimethylammonium chloride Drugs 0.000 claims description 17
- 238000010438 heat treatment Methods 0.000 claims description 14
- 239000000725 suspension Substances 0.000 claims description 13
- 239000000203 mixture Substances 0.000 claims description 12
- QTBSBXVTEAMEQO-UHFFFAOYSA-N Acetic acid Chemical compound CC(O)=O QTBSBXVTEAMEQO-UHFFFAOYSA-N 0.000 claims description 11
- 238000000034 method Methods 0.000 claims description 8
- 239000002253 acid Substances 0.000 claims description 7
- XNCOSPRUTUOJCJ-UHFFFAOYSA-N Biguanide Chemical group NC(N)=NC(N)=N XNCOSPRUTUOJCJ-UHFFFAOYSA-N 0.000 claims description 5
- 229940123208 Biguanide Drugs 0.000 claims description 5
- 229960000583 acetic acid Drugs 0.000 claims description 5
- 238000001816 cooling Methods 0.000 claims description 5
- 238000002156 mixing Methods 0.000 claims description 5
- 238000002360 preparation method Methods 0.000 claims description 5
- 150000003242 quaternary ammonium salts Chemical class 0.000 claims description 5
- 230000008961 swelling Effects 0.000 claims description 5
- 239000012362 glacial acetic acid Substances 0.000 claims description 4
- BJEPYKJPYRNKOW-UHFFFAOYSA-N malic acid Chemical compound OC(=O)C(O)CC(O)=O BJEPYKJPYRNKOW-UHFFFAOYSA-N 0.000 claims description 4
- 238000010521 absorption reaction Methods 0.000 claims description 3
- 150000001413 amino acids Chemical class 0.000 claims description 2
- 229960000686 benzalkonium chloride Drugs 0.000 claims description 2
- XIWFQDBQMCDYJT-UHFFFAOYSA-M benzyl-dimethyl-tridecylazanium;chloride Chemical compound [Cl-].CCCCCCCCCCCCC[N+](C)(C)CC1=CC=CC=C1 XIWFQDBQMCDYJT-UHFFFAOYSA-M 0.000 claims description 2
- 229960002152 chlorhexidine acetate Drugs 0.000 claims description 2
- UMGXUWVIJIQANV-UHFFFAOYSA-M didecyl(dimethyl)azanium;bromide Chemical compound [Br-].CCCCCCCCCC[N+](C)(C)CCCCCCCCCC UMGXUWVIJIQANV-UHFFFAOYSA-M 0.000 claims description 2
- DDXLVDQZPFLQMZ-UHFFFAOYSA-M dodecyl(trimethyl)azanium;chloride Chemical compound [Cl-].CCCCCCCCCCCC[N+](C)(C)C DDXLVDQZPFLQMZ-UHFFFAOYSA-M 0.000 claims description 2
- XJWSAJYUBXQQDR-UHFFFAOYSA-M dodecyltrimethylammonium bromide Chemical compound [Br-].CCCCCCCCCCCC[N+](C)(C)C XJWSAJYUBXQQDR-UHFFFAOYSA-M 0.000 claims description 2
- RARSHUDCJQSEFJ-UHFFFAOYSA-N p-Hydroxypropiophenone Chemical compound CCC(=O)C1=CC=C(O)C=C1 RARSHUDCJQSEFJ-UHFFFAOYSA-N 0.000 claims description 2
- MCSINKKTEDDPNK-UHFFFAOYSA-N propyl propionate Chemical compound CCCOC(=O)CC MCSINKKTEDDPNK-UHFFFAOYSA-N 0.000 claims description 2
- 238000004659 sterilization and disinfection Methods 0.000 abstract description 27
- 230000009471 action Effects 0.000 abstract description 14
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- 241000700605 Viruses Species 0.000 abstract description 8
- 238000009395 breeding Methods 0.000 abstract description 6
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- 231100000989 no adverse effect Toxicity 0.000 abstract 1
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- 241000191967 Staphylococcus aureus Species 0.000 description 2
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- 229910052751 metal Inorganic materials 0.000 description 2
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- LKAPTZKZHMOIRE-KVTDHHQDSA-N (2s,3s,4s,5r)-3,4-dihydroxy-5-(hydroxymethyl)oxolane-2-carbaldehyde Chemical compound OC[C@H]1O[C@H](C=O)[C@@H](O)[C@@H]1O LKAPTZKZHMOIRE-KVTDHHQDSA-N 0.000 description 1
- 241001545522 Aguacate virus Species 0.000 description 1
- OYPRJOBELJOOCE-UHFFFAOYSA-N Calcium Chemical compound [Ca] OYPRJOBELJOOCE-UHFFFAOYSA-N 0.000 description 1
- 229920002101 Chitin Polymers 0.000 description 1
- 206010012735 Diarrhoea Diseases 0.000 description 1
- 241000192125 Firmicutes Species 0.000 description 1
- 108010010803 Gelatin Proteins 0.000 description 1
- 241000283977 Oryctolagus Species 0.000 description 1
- 241001135549 Porcine epidemic diarrhea virus Species 0.000 description 1
- 241001135989 Porcine reproductive and respiratory syndrome virus Species 0.000 description 1
- ZLMJMSJWJFRBEC-UHFFFAOYSA-N Potassium Chemical compound [K] ZLMJMSJWJFRBEC-UHFFFAOYSA-N 0.000 description 1
- 206010040880 Skin irritation Diseases 0.000 description 1
- 241000191940 Staphylococcus Species 0.000 description 1
- 241000282887 Suidae Species 0.000 description 1
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- 230000002378 acidificating effect Effects 0.000 description 1
- 239000013543 active substance Substances 0.000 description 1
- 125000003277 amino group Chemical group 0.000 description 1
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- 229910052791 calcium Inorganic materials 0.000 description 1
- 210000000170 cell membrane Anatomy 0.000 description 1
- 210000002421 cell wall Anatomy 0.000 description 1
- LKAPTZKZHMOIRE-UHFFFAOYSA-N chitose Natural products OCC1OC(C=O)C(O)C1O LKAPTZKZHMOIRE-UHFFFAOYSA-N 0.000 description 1
- 239000000084 colloidal system Substances 0.000 description 1
- 238000013329 compounding Methods 0.000 description 1
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- 230000000120 cytopathologic effect Effects 0.000 description 1
- 231100000263 cytotoxicity test Toxicity 0.000 description 1
- 230000000249 desinfective effect Effects 0.000 description 1
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- 229920006008 lipopolysaccharide Polymers 0.000 description 1
- 238000012423 maintenance Methods 0.000 description 1
- 239000011976 maleic acid Substances 0.000 description 1
- 210000004400 mucous membrane Anatomy 0.000 description 1
- 238000006386 neutralization reaction Methods 0.000 description 1
- 231100000028 nontoxic concentration Toxicity 0.000 description 1
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- 239000002574 poison Substances 0.000 description 1
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Classifications
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N33/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic nitrogen compounds
- A01N33/02—Amines; Quaternary ammonium compounds
- A01N33/12—Quaternary ammonium compounds
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N25/00—Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests
- A01N25/22—Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests containing ingredients stabilising the active ingredients
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/02—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms
- A01N43/04—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom
- A01N43/14—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom six-membered rings
- A01N43/16—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom six-membered rings with oxygen as the ring hetero atom
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N47/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom not being member of a ring and having no bond to a carbon or hydrogen atom, e.g. derivatives of carbonic acid
- A01N47/40—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom not being member of a ring and having no bond to a carbon or hydrogen atom, e.g. derivatives of carbonic acid the carbon atom having a double or triple bond to nitrogen, e.g. cyanates, cyanamides
- A01N47/42—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom not being member of a ring and having no bond to a carbon or hydrogen atom, e.g. derivatives of carbonic acid the carbon atom having a double or triple bond to nitrogen, e.g. cyanates, cyanamides containing —N=CX2 groups, e.g. isothiourea
- A01N47/44—Guanidine; Derivatives thereof
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01P—BIOCIDAL, PEST REPELLANT, PEST ATTRACTANT OR PLANT GROWTH REGULATORY ACTIVITY OF CHEMICAL COMPOUNDS OR PREPARATIONS
- A01P1/00—Disinfectants; Antimicrobial compounds or mixtures thereof
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Wood Science & Technology (AREA)
- General Health & Medical Sciences (AREA)
- Plant Pathology (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Pest Control & Pesticides (AREA)
- Environmental Sciences (AREA)
- Agronomy & Crop Science (AREA)
- Dentistry (AREA)
- Chemical & Material Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Toxicology (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The invention relates to a novel chitosan compound type cationic disinfectant suitable for high-power dilution, which is prepared from the following raw material components: cationic surfactant disinfectant, chitosan, compound amino acid and water; the chitosan has a weight average molecular weight of at least 250,000 daltons; the compound amino acid comprises glycine, proline, hydroxyproline, alanine, arginine, aspartic acid and glutamic acid. The disinfectant provided by the invention has good sterilization effect and high safety, and is suitable for sterilization and disinfection of multiple purposes such as object surface sterilization, hand sterilization, livestock and poultry house breeding environment sterilization, personnel channel sterilization and the like. The disinfectant has broad spectrum effect, namely has stronger killing capacity on G +, G-and viruses, has low action concentration and has no adverse effect on the safety of people and livestock.
Description
Technical Field
The invention belongs to the technical field of disinfectants, and particularly relates to a novel chitosan compound type cationic disinfectant suitable for high-power dilution.
Background
The livestock and poultry breeding is economic on a large scale, the breeding density is continuously improved, and the negative effect brought by the improvement is that the psychological pressure of animal groups is easily caused, so that the urgent phenomenon is generated, and the animals in the animal house have the characteristics of low immunity, easy disease infection and the like. And due to the influence of multiple factors on the technical level of cultivation management execution, the animals are more easily infected with diseases and are rapidly spread.
The disinfection is one of the first important points in the cultivation management, and also occupies a certain cultivation cost. The existing disinfectant generally has toxicity and irritation to mucous membranes, respiratory tracts and the like of organisms to a certain extent, also has metal corrosivity, inflammability, even toxicity and the like, and is not suitable for being used in an environment with one object, so that a farm can be disinfected when needing an empty room, can not be disinfected during the breeding period, and is easy to damage the environment after being used for a long time. In order to reduce the irritation, toxicity and cost of the disinfectant, the disinfectant can be diluted for use, but the existing disinfectant has the problem that the disinfection and sterilization effect is sharply reduced after being diluted by a high factor, so that the requirements of the disinfection technical code of the ministry of health are not met.
Therefore, a disinfectant which can maintain an excellent disinfection and sterilization effect after being diluted by a high power is still lacking in the market at present, so that the effect, the cost and the safety are considered, and the problem to be solved in the field of the disinfectant at present is solved.
Disclosure of Invention
Aiming at the problems in the prior art, the invention provides a novel chitosan compound type cationic disinfectant which is suitable for high-power dilution, and has the advantages of low action concentration, good sterilization effect, high safety and wide application.
In order to achieve the purpose, the invention adopts the following technical scheme:
a novel chitosan compound type cationic disinfectant suitable for high-power dilution is prepared from the following raw material components: cationic surfactant disinfectant, chitosan, compound amino acid and water; the chitosan has a weight average molecular weight of at least 250,000 daltons; the compound amino acid comprises glycine, proline, hydroxyproline, alanine, arginine, aspartic acid and glutamic acid.
The cationic surfactant adopted by the invention can form positively charged surface active ions in water, and the positively charged surface active ions can be adsorbed on the negatively charged bacteria surface to destroy cell membranes, so that the permeability is changed, the metabolism is hindered, and the thallus is broken and killed. The cationic surfactant disinfectant is a biguanide disinfectant and a quaternary ammonium salt disinfectant; the biguanide disinfectant is selected from one or more of polyhexamethylene biguanide hydrochloride, chlorhexidine digluconate and chlorhexidine acetate, and preferably polyhexamethylene biguanide hydrochloride; the quaternary ammonium salt disinfectant is selected from one or more of benzalkonium bromide, benzalkonium chloride, didecyl dimethyl ammonium bromide, dodecyl trimethyl ammonium chloride and dodecyl trimethyl ammonium bromide, and preferably benzalkonium bromide and didecyl dimethyl ammonium chloride.
The chitosan adopted by the invention is extracted from marine organism chitin, has a large amount of amino groups (NH3+), can be protonated in an acidic environment, is an active substance with polarity and positive charge, can be combined with lipoteichoic acid or lipopolysaccharide with negative charge in the cell wall of bacteria through electrostatic attraction to block normal metabolism positive energy, and simultaneously destroys the cell surface layer structure to enable calcium (Ca) 2+ ) Potassium (K) + ) Plasma ions are lost outwards, cell permeability is changed, internal pressure and external pressure are unbalanced, and pathogeny death is caused. It has been found that when the chitosan molecular weight is within the range defined in the present invention, excellent antimicrobial efficacy can be exhibited at low concentrations. This antimicrobial effect is non-specific and therefore has a wide range of applicability.
The compound amino acid adopted by the invention can improve the application property and stability of the formula and has a gain effect on the formula. Specifically, the compound amino acid has thickening property, and the physical characteristics have the following advantages after long-time storage and experimental observation: the added groups have lower viscosity, less loss and higher temperature resistance (the added groups are similar to colloid under low temperature environment, and the groups without the added groups are frozen into solid). The antibacterial property is improved by adding compound amino acid through experimental observation.
The chitosan compound type cationic disinfectant suitable for high-power dilution is preferably prepared from the following raw material components in percentage by weight: benzalkonium bromide 2.5-5 wt%, polyhexamethylene biguanide hydrochloride 1-2 wt%, didecyl dimethyl ammonium chloride 0.5-1 wt%, chitosan 0.34-0.68 wt%, compound amino acid 0.075-0.145 wt% and water for the rest. Further preferably, the material is prepared from the following raw materials in percentage by weight: 5% of benzalkonium bromide, 2% of polyhexamethylene biguanide hydrochloride, 1% of didecyldimethyl ammonium chloride, 0.68% of chitosan, 0.145% of compound amino acid and the balance of water. Further preferably, the material is prepared from the following raw materials in percentage by weight: benzalkonium bromide 2.5%, polyhexamethylene biguanide hydrochloride 1%, didecyl dimethyl ammonium chloride 0.5%, chitosan 0.34%, compound amino acid 0.075%, and the balance of water.
The preparation method of the novel chitosan compound type cationic disinfectant suitable for high-power dilution comprises the following steps:
(1) adding part of water into the compound amino acid to uniformly absorb water and expand to obtain a solution A;
(2) sequentially adding the solution A and chitosan into a stirring tank, stirring and heating to uniformly disperse to obtain a suspension;
(3) slowly adding an acid solution into the suspension, naturally cooling and continuously stirring until the solution is completely dissolved to obtain a solution B;
(4) mixing benzalkonium bromide, polyhexamethylene biguanide hydrochloride and didecyldimethyl ammonium chloride to obtain a mixture; adding the solution B into the mixture, adding the residual water, and stirring under a heating condition to uniformly dissolve the mixture to obtain the novel chitosan composite cationic disinfectant suitable for high-power dilution.
Preferably, in the step (1), the mass ratio of the compound amino acid to the water is 2.9:108, the water absorption swelling time is 60min, if the water absorption swelling time is insufficient, the solubility is insufficient, and the solubility obtained after the chitosan is added in the subsequent step (2) can generate a plurality of obviously insoluble particles, so that the application is not facilitated.
Preferably, in the step (2), the heating is carried out to 50-60 ℃, and the stirring is carried out for 10min at a stirring speed of 150 rpm.
Preferably, in the step (3), the acid solution is DL-Malic acid (DL-maleic acid C) 4 H 6 O 5 ) Or glacial Acetic Acid (Acetic Acid C) 2 H 4 O 2 ) When the acid solution is added, the solution resistance is increased to 4.5-6N/cm, and the stirring is continued until the resistance is reduced to 1.6-2.0N/cm. The effect of the acid is to dissolve the chitose.
The invention has the beneficial effects that:
1. the disinfectant provided by the invention can generate an antibacterial synergistic effect by compounding the cationic surfactant disinfectant, the chitosan with a specific molecular weight and the compound amino acid with a specific composition, so that the sterilizing titer is improved, the using amount is reduced, the finally prepared disinfectant still keeps an excellent sterilizing effect after being diluted by a high power, and the sterilizing effect on gram negative bacteria and gram positive bacteria including escherichia coli and staphylococcus aureus can meet the requirement of 'sterilizing technical specification' of the Ministry of health. Through detection, after the disinfectant is diluted according to the volume ratio of the stock solution to water of 1:4000, the log value of the killing rate of the disinfectant on escherichia coli, staphylococcus, porcine reproductive and respiratory syndrome virus and porcine epidemic diarrhea virus is more than 5 after 15 minutes.
2. The disinfectant provided by the invention has the advantages that the safety meets the disinfection technical specification, no pungent smell exists, metal is not corroded, no dyeing or fading exists, the disinfectant is non-toxic through the mouth, no stimulation is caused to the skin, and the disinfectant meets the use scene and the requirement of an animal farm. The disinfectant is suitable for disinfection and sterilization of multiple purposes such as object surface disinfection, hand disinfection, livestock and poultry house breeding environment disinfection, personnel channel disinfection and the like, and particularly can achieve the effect of seedling killing when being used for disinfection of livestock and poultry house breeding environment, the sterilization rate is more than 90%, meanwhile, the original safety of the product in the human medical level is kept, and the application standard of disinfection with livestock can be achieved.
Detailed Description
In order to make the objects, technical solutions and advantages of the present invention more apparent, the technical solutions of the present invention will be described in detail below. It is to be understood that the described embodiments are merely a few embodiments of the invention, and not all embodiments. All other embodiments, which can be derived by a person skilled in the art from the examples given herein without any inventive step, are within the scope of the present invention.
In the following examples, benzalkonium bromide was obtained from the browning of Tyco (content 95-105%); polyhexamethylene biguanide hydrochloride was purchased from Qingdao Fumeisi (content 20%); didecyl dimethyl ammonium chloride was purchased from linyi lusson (content 80%); the chitosan is purchased from Qingdao Honghai biotechnology limited company, has the product model of SC12237021201880, and has the molecular weight of 280,000 daltons to 800,000 daltons; the complex amino acids were purchased from Roselo (Guangdong) gelatin, Inc. under the trade designation 150LB 8.
Example 1
The embodiment provides a novel chitosan compound type cationic disinfectant suitable for high-power dilution, which is prepared from the following raw material components in percentage by weight:
5% of benzalkonium bromide, 2% of polyhexamethylene biguanide hydrochloride, 1% of didecyldimethyl ammonium chloride, 0.68% of chitosan, 0.145% of compound amino acid and the balance of water.
The preparation method comprises the following steps:
(1) adding water (2.9: 108) into the compound amino acid, and uniformly absorbing water to swell for 60min to obtain a solution A;
(2) adding the solution A and chitosan into a stirring tank in sequence, heating to 55 ℃, and stirring at 150rpm for 10min to obtain a suspension;
(3) slowly adding 99.0 wt/% DL-malic acid which accounts for 10.7 ‰ of the total weight of the suspension into the suspension, stopping heating, naturally cooling, continuously stirring for 2h until completely dissolving, and reducing resistance to 1.6N/cm to obtain solution B;
(4) mixing benzalkonium bromide, polyhexamethylene biguanide hydrochloride and didecyl dimethyl ammonium chloride to obtain a mixture; adding the solution B into the mixture, adding the residual water, heating to 55 ℃, and stirring to dissolve uniformly to obtain the novel chitosan composite cationic disinfectant suitable for high-power dilution.
Example 2
The embodiment provides a novel chitosan compound type cationic disinfectant suitable for high-power dilution, which is prepared from the following raw material components in percentage by weight:
benzalkonium bromide 2.5%, polyhexamethylene biguanide hydrochloride 1%, didecyl dimethyl ammonium chloride 0.5%, chitosan 0.34%, compound amino acid 0.075%, and the balance of water.
The preparation method comprises the following steps:
(1) adding water (2.9: 108) into the compound amino acid, and uniformly absorbing water and swelling for 60min to obtain a solution A;
(2) adding the solution A and chitosan into a stirring tank in sequence, heating to 55 ℃, and stirring at 150rpm for 10min to obtain a suspension;
(3) slowly adding 100.5 wt/% DL-malic acid accounting for 5.35 ‰ of the total weight of the suspension into the suspension, stopping heating, naturally cooling, continuously stirring for 2 hr until completely dissolving, and reducing resistance to 1.6N/cm to obtain solution B;
(4) mixing benzalkonium bromide, polyhexamethylene biguanide hydrochloride and didecyldimethyl ammonium chloride to obtain a mixture; adding the solution B into the mixture, adding the residual water, heating to 55 ℃, and stirring to dissolve uniformly to obtain the novel chitosan composite cationic disinfectant suitable for high-power dilution.
Example 3
The embodiment provides a novel chitosan compound type cationic disinfectant suitable for high-power dilution, which is prepared from the following raw material components in percentage by weight:
3% of benzalkonium bromide, 1.5% of polyhexamethylene biguanide hydrochloride, 0.8% of didecyldimethyl ammonium chloride, 0.52% of chitosan, 0.125% of compound amino acid and the balance of water.
The preparation method comprises the following steps:
(1) adding water (2.9: 108) into the compound amino acid, and uniformly absorbing water and swelling for 60min to obtain a solution A;
(2) adding the solution A and chitosan into a stirring tank in sequence, heating to 55 ℃, and stirring at 150rpm for 10min to obtain a suspension;
(3) slowly adding glacial acetic acid with the concentration of 98.0 wt/% of 8.025 per mill of the total weight of the suspension into the suspension, stopping heating, naturally cooling, continuously stirring for 2h until the glacial acetic acid is completely dissolved, and reducing the resistance to 2.0N/cm to obtain a solution B;
(4) mixing benzalkonium bromide, polyhexamethylene biguanide hydrochloride and didecyldimethyl ammonium chloride to obtain a mixture; adding the solution B into the mixture, adding the residual water, and stirring to dissolve uniformly to obtain the novel chitosan compound type cationic disinfectant suitable for high-power dilution.
Comparative example 1
The difference from example 1 is that the chitosan has a molecular weight of 3200.0-250,000 dal.
Comparative example 2
The only difference from example 1 is the removal of chitosan.
Test examples
First, efficacy test
1. Test No.)
1.1 strains: KF-Ecoli
1.2 Experimental methods: reference GB 15979-2002C 3.2
1.3 colony counting and bacterial liquid action concentration configuration: the plate counting method is used for counting the content of the desired strain, and diluting with a common broth to an action concentration (about 10) 8 CFU/mL)
1.4 Experimental results:
TABLE 1
2. Test No. two
2.1 test strains: KF-Ecoli
2.2 Experimental methods: reference GB 15979-2002C 3.2
2.3 colony counting and bacterial liquid action concentration configuration: the plate count method was performed to count the content of the desired strain, and the strain was diluted with a normal broth to the effect concentration (about 108CFU/mL) according to the count result
2.4 Experimental results:
TABLE 2
3. Experiment three
3.1 strains: staphylococcus aureus
3.2 Experimental methods: reference GB 15979-2002C 3.2
3.3 colony counting and bacterial liquid action concentration configuration: the plate counting method is used for counting the content of the desired strain, and diluting with a common broth to an action concentration (about 10) 8 CFU/mL)
3.4 Experimental results:
TABLE 3
4. Experiment four
4.1 strains: KF-Ecoli
4.2 Experimental methods: reference GB 15979-2002C 3.2
4.3 colony counting and bacterial liquid action concentration configuration: the plate counting method is used for counting the content of the desired strain, and diluting with a common broth to an action concentration (about 10) 8 CFU/mL)
4.4 results of the experiment:
TABLE 4
5. Experiment five
5.1 disinfecting product: example 1
5.2 poison bead: blue ear disease virus (NADX30-L), epidemic diarrhea virus (DR13)
5.3 method (refer to TCID50 method)
a. Virus titer determination and action concentration configuration: the TCID50 method counts the titer of the desired beads, and dilutes the titer to the action concentration (about 10) 6 TCID50/mL)
b. Preparing the action concentration of the disinfectant: prepared according to 2 times of the required concentration (1000 times) for standby
c. The virus liquid and the disinfectant have the following effects: 0.5ml of test beads (concentration about 10) was aspirated 6 TCID50/mL) and 0.5mL of disinfectant solution with 2 times concentration at 20 ℃, adding 0.5mL of D/E neutralization broth for 10min after 1min, 2min, 5min, 15min and 30min of action, adding into cells, culturing at 37 ℃ and 5% CO2 for 1-7 days, and observing cytopathic effect.
TABLE 5
6. Test six
6.1. Virus: the high-pathogenicity African swine fever virus Pig/HLJ/2018 strain is used as a framework to construct infectious clone virus beads carrying GFP labels
6.2. Porcine Primary Alveolar Macrophages (PAMs): porcine primary alveolar macrophages are isolated from 30-40 day SPF pigs derived from the experimental animal base of the Harbin veterinary institute.
6.3. Disinfectant cytotoxicity test on PAMs
3 test concentrations of the disinfectant of the present invention (example 1) were diluted in multiples of the cell maintenance solution and seeded onto PAMs cells at 37 ℃ with 5% CO 2 The cells were incubated in an incubator and the cell status was observed every day.
TABLE 6 maximum non-toxic concentration of disinfectant
| Concentration of disinfectant | 1:50 | 1:100 | 1:200 |
| Dilution factor | 1:20 | 1:10 | 1:5 |
6.4. Disinfectant for killing African swine fever virus
The disinfectant (example 1) provided by the invention is used for detecting the inactivation effect of African swine fever virus at 25 ℃ by adopting three different concentrations and four different action times. The result judgment standard is that the test group has no obvious fluorescent signal, which proves that the African swine fever virus is completely inactivated by the disinfectant.
TABLE 7 fluorescent observations of the mode of disinfectant immersion at 25 ℃ after interaction with ASFV
Second, safety test
1. Virus: the high-pathogenicity African swine fever virus Pig/HLJ/2018 strain is used as a framework to construct infectious clone virus beads carrying GFP labels
2. The inspection basis is as follows: disinfection technical Specification (2002 edition) 2.3.1, Disinfection technical Specification (2002 edition) 2.3.3
3. The experimental conclusion is that:
TABLE 8 acute oral toxicity test results
| Dosage (mg/kg bw) | Sex | Animal number (only) | Number of dead animals | Mortality (%) |
| 5000 | Female | 10 | 0 | 0 |
| 5000 | Male sex | 10 | 0 | 0 |
The KM mice have no abnormal symptoms and death within 14 days of infection, and no abnormality is found after the test observation through gross anatomical examination of the tested animals. Acute oral toxicity LD of disinfectant stock solution of the invention on KM mice 50 >5000mg/kg bw, the acute oral toxicity test is actually nontoxic and meets the requirements of disinfection technical specifications (2002 edition).
TABLE 9 Scoring results of multiple complete skin irritation tests
Under the experimental condition, the disinfectant (1mL:199.2mL, 5 times of the highest application concentration) has no irritation to the stimulation intensity of a new Zealand rabbit multiple complete skin stimulation test, and meets the requirements of disinfection technical Specifications (2002 edition).
The above description is only for the specific embodiments of the present invention, but the scope of the present invention is not limited thereto, and any person skilled in the art can easily conceive of the changes or substitutions within the technical scope of the present invention, and all the changes or substitutions should be covered within the scope of the present invention. Therefore, the protection scope of the present invention shall be subject to the protection scope of the appended claims.
Claims (10)
1. A novel chitosan compound type cationic disinfectant suitable for high-power dilution is characterized by comprising the following raw material components: cationic surfactant disinfectant, chitosan, compound amino acid and water; the chitosan has a weight average molecular weight of at least 250,000 daltons; the compound amino acid comprises glycine, proline, hydroxyproline, alanine, arginine, aspartic acid and glutamic acid.
2. The novel chitosan composite cationic disinfectant suitable for high-power dilution use as claimed in claim 1, wherein the cationic surfactant disinfectant is a biguanide disinfectant and a quaternary ammonium salt disinfectant; the biguanide disinfectant is one or more of polyhexamethylene biguanide hydrochloride, chlorhexidine digluconate and chlorhexidine acetate; the quaternary ammonium salt disinfectant is one or more of benzalkonium bromide, benzalkonium chloride, didecyl dimethyl ammonium bromide, dodecyl trimethyl ammonium chloride and dodecyl trimethyl ammonium bromide.
3. The novel chitosan composite cationic disinfectant suitable for high dilution according to claim 2, wherein the biguanide disinfectant is polyhexamethylene biguanide hydrochloride, and the quaternary ammonium salt disinfectant is benzalkonium bromide and didecyldimethyl ammonium chloride.
4. The novel chitosan compound type cationic disinfectant suitable for high-power dilution use as claimed in claim 3, is characterized by being prepared from the following raw materials in percentage by weight:
benzalkonium bromide 2.5-5 wt%, polyhexamethylene biguanide hydrochloride 1-2 wt%, didecyl dimethyl ammonium chloride 0.5-1 wt%, chitosan 0.34-0.68 wt%, compound amino acid 0.075-0.145 wt% and water for the rest.
5. The novel chitosan compound type cationic disinfectant suitable for high-power dilution use as claimed in claim 4, is characterized by being prepared from the following raw materials in percentage by weight:
5% of benzalkonium bromide, 2% of polyhexamethylene biguanide hydrochloride, 1% of didecyldimethyl ammonium chloride, 0.68% of chitosan, 0.145% of compound amino acid and the balance of water.
6. The novel chitosan compound type cationic disinfectant suitable for high-power dilution use as claimed in claim 4, is characterized by being prepared from the following raw materials in percentage by weight:
benzalkonium bromide 2.5%, polyhexamethylene biguanide hydrochloride 1%, didecyl dimethyl ammonium chloride 0.5%, chitosan 0.34%, compound amino acid 0.075%, and the balance of water.
7. A method for preparing a novel chitosan complex cationic disinfectant suitable for high dilution as claimed in any one of claims 4 to 6, which comprises the following steps:
(1) adding part of water into the compound amino acid to uniformly absorb water and expand to obtain a solution A;
(2) sequentially adding the solution A and chitosan into a stirring tank, stirring and heating to uniformly disperse to obtain a suspension;
(3) slowly adding an acid solution into the suspension, naturally cooling and continuously stirring until the solution is completely dissolved to obtain a solution B;
(4) mixing benzalkonium bromide, polyhexamethylene biguanide hydrochloride and didecyldimethyl ammonium chloride to obtain a mixture; adding the solution B into the mixture, adding the residual water, and stirring under a heating condition to dissolve uniformly to obtain the novel chitosan composite cationic disinfectant suitable for high-power dilution.
8. The preparation method of the novel chitosan composite cationic disinfectant suitable for high-power dilution as claimed in claim 7, wherein in the step (1), the water absorption swelling time is 60min, and the mass ratio of the composite amino acid to the water is 2.9: 108.
9. The method for preparing the novel chitosan composite cationic disinfectant suitable for high-power dilution use as claimed in claim 7, wherein in the step (2), the heating is carried out to 50-60 ℃, and the stirring is carried out for 10min at a stirring speed of 150 rpm.
10. The method for preparing the novel chitosan composite cationic disinfectant suitable for high dilution according to claim 7, wherein in the step (3), the acid solution is DL-malic acid or glacial acetic acid, and the stirring is continued until the resistance is reduced to 1.6-2.0N/cm.
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Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101933512A (en) * | 2010-07-05 | 2011-01-05 | 高旭 | Disinfectant capable of being used with high dilution multiples, preparation method and application |
| US20110236504A1 (en) * | 2008-05-01 | 2011-09-29 | Panacea Disinfectant Co. Limited | Versatile disinfectant |
| CN103732225A (en) * | 2010-03-23 | 2014-04-16 | Gojo工业公司 | Antimicrobial compositions |
| CN107466207A (en) * | 2015-04-02 | 2017-12-12 | 拜奥特罗尔有限公司 | Antimicrobial compositions |
| CN109169653A (en) * | 2018-10-24 | 2019-01-11 | 湖北荷普药业股份有限公司 | A kind of cation composite disinfectant and its application |
| CN111616148A (en) * | 2020-05-28 | 2020-09-04 | 江苏雪豹日化有限公司 | High-efficiency disinfectant |
| JP2021107344A (en) * | 2019-12-27 | 2021-07-29 | 小林製薬株式会社 | Disinfectant |
| CN113940358A (en) * | 2021-09-29 | 2022-01-18 | 中科朗劢技术有限公司 | Efficient alcohol-free guanidine compound disinfectant and preparation method thereof |
-
2022
- 2022-07-29 CN CN202210909187.1A patent/CN115119841A/en active Pending
Patent Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20110236504A1 (en) * | 2008-05-01 | 2011-09-29 | Panacea Disinfectant Co. Limited | Versatile disinfectant |
| CN103732225A (en) * | 2010-03-23 | 2014-04-16 | Gojo工业公司 | Antimicrobial compositions |
| CN101933512A (en) * | 2010-07-05 | 2011-01-05 | 高旭 | Disinfectant capable of being used with high dilution multiples, preparation method and application |
| CN107466207A (en) * | 2015-04-02 | 2017-12-12 | 拜奥特罗尔有限公司 | Antimicrobial compositions |
| CN109169653A (en) * | 2018-10-24 | 2019-01-11 | 湖北荷普药业股份有限公司 | A kind of cation composite disinfectant and its application |
| JP2021107344A (en) * | 2019-12-27 | 2021-07-29 | 小林製薬株式会社 | Disinfectant |
| CN111616148A (en) * | 2020-05-28 | 2020-09-04 | 江苏雪豹日化有限公司 | High-efficiency disinfectant |
| CN113940358A (en) * | 2021-09-29 | 2022-01-18 | 中科朗劢技术有限公司 | Efficient alcohol-free guanidine compound disinfectant and preparation method thereof |
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