CN115040582B - 含两面针提取物的中药护龈口腔护理用品 - Google Patents
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Abstract
本发明提供了一种含两面针提取物的中药护龈口腔护理用品，含有中药活性成分，中药活性成分由两面针提取物、柚皮苷和断血流提取物，按重量百分比计，两面针提取物含量为0.1~10%，柚皮苷含量为0.01%～5%，断血流提取物含量为0.01~8%。本发明将特定配比的两面针提取物、柚皮苷和断血流提取物添加至口腔护理用品中，上述成分相互协同，可起到抑菌、止血等作用，能有效抑牙菌斑、缓解牙龈红肿、牙龈出血等不适状况。
Description
技术领域
本发明涉及口腔护理用品技术领域，尤其涉及一种中药护龈口腔护理用品。
背景技术
龋齿、牙本质过敏、牙龈炎、牙龈红肿、牙龈出血、牙周炎、口臭、口腔溃疡、牙痛等是常见口腔疾病。其中前两项与牙齿相关，其余的均与牙龈牙周有关。牙龈炎是牙龈疾病的早期阶段，主要病因是牙齿上累积的牙菌斑(由细菌、唾液和食物残渣构成的黏性物质)，其表现为牙龈红肿和刷牙时易出血，如果治疗得当，使用合适的口腔护理用品，恢复口腔卫生，牙龈可以恢复健康状态。
近年在环保、安全、回归大自然的影响下，具备功能性的植物提取物产品备受青睐。口腔护理用品作为人们日常生活不可缺少的用品，在清洁功能之外，更注重其具有安全、有效的功效性，能保持口腔卫生、口气清新、抑制和减少口腔细菌、减轻牙龈问题等。
植物提取物作为功效成分添加至口腔护理用品中，具有资源广泛、毒副作用小、不易产生耐药性的特点，同时对效抑牙菌斑、缓解牙龈红肿、牙龈出血等具有很好的疗效。研究一种以植物提取物为功效成分、保护牙龈健康的口腔护理用品对于人们的口腔卫生来说是十分有意义的。
发明内容
本发明提供了一种含两面针提取物的中药护龈口腔护理用品，将特定配比的两面针提取物、柚皮苷和断血流提取物添加至口腔护理用品中，上述成分相互协同，可起到抑菌、止血等作用，能有效抑牙菌斑、缓解牙龈红肿、牙龈出血等不适状况。
为实现上述目的，本发明的技术方案为：
一种含两面针提取物的中药护龈口腔护理用品，其特征在于：
口腔护理用品含有中药活性成分，中药活性成分由两面针提取物、柚皮苷和断血流提取物，按重量百分比计，两面针提取物含量为0.1～10％，柚皮苷含量为0.01％～5％，断血流提取物含量为0.01～8％。
优选的，两面针提取物含量为0.1～2.0％。
优选的，柚皮苷提取物含量为0.02～1.0％。
优选的，断血流提取物含量为0.05～5.0％。
优选的，所述两面针提取物、所述柚皮苷和所述断血流提取物均为乙醇提取物。
更优选的，所述两面针提取物和所述断血流提取物为原药用乙醇提取、浓缩后，分别得到对应提取物的重量与原药质量的比例为0.5～2:1。更优选的，所述乙醇为体积分数是50～95％的乙醇。
优选的，所述柚皮苷为原药用乙醇提取、浓缩、提纯，真空干燥，得到柚皮苷粉末。更优选的，所述乙醇为体积分数是50～95％的乙醇。
优选地，柚皮苷纯度≥98％。
优选的，所述口腔护理用品为牙膏、漱口水、牙粉、口腔喷雾剂、口腔清洗剂或口香糖。
两面针为芸香科植物两面针Zanthoxylum nitidum(Roxb.)DC.的干燥根，味苦、辛，性平。具有活血化瘀、行气止痛、祛风通络、解毒消肿等功效，可用于跌扑损伤、胃痛、牙痛、风湿痹痛等。
柚皮苷是一种双氢黄酮类化合物，具有抗炎、镇痛、抗病毒、抑菌、抗过敏、抗溃疡、改善局部微循环和营养供给等。
断血流为唇形科植物灯笼草Cliruo podium polycephalum(Vani-ot)C.Y.Wu etHsuan或风轮菜Clinopodium chinense(Benth)Q.Kuntze的干燥地上部分，微苦、涩，凉。凉血止血，可用于崩漏，尿血，鼻衄，牙龈出血，创伤出血；子宫肌瘤出血等。
本发明专注于解决牙龈问题，通过两面针提取物、柚皮苷和断血流提取物的多重作用，不但能减轻牙龈炎症、出血等症状，而且能抑制口腔细菌，安全有效地预防口腔牙龈问题。
本发明采用特定配比的两面针提取物、柚皮苷和断血流提取物添加至口腔护理用品中，经药理试验证明，上述成分相互协同，本发明的口腔护理用品对金黄色葡萄球菌、白色念珠菌具有较强的抑菌效果，对牙龈卟啉单胞菌、变形链球菌的生物细胞生长曲线具有较强的抑制效果，同时还有明显的抗炎和止血效果，对保护牙龈健康、维护口腔卫生具有积极作用。
附图说明
图1是牙龈卟啉单胞菌生长曲线图。
图2是变形链球菌生长曲线图。
具体实施方式
以下结合具体实施例对本发明作进一步说明，但本发明的保护范围不限于以下实施例。
本实施例采用的两面针提取物、断血流提取物，为分别采用两面针、断血流原药与75％vol乙醇按重量比1:5的比例进行加热回流提取，将提取物浓缩至与原药质量比例为1:1。
柚皮苷采用原药与95％vol乙醇按重量比1:20的比例，在≤50℃条件下回流提取，滤液经过浓缩提纯，真空干燥，得到纯度≥98％的柚皮苷。
在制备口腔护理用品时加入两面针提取物、柚皮苷、断血流提取物作为抑菌、抗炎、止血的功效成分。
一、含两面针提取物、柚皮苷、断血流提取物的中药护龈口腔护理用品(以在牙膏中制作为例)
实施例1至5中的牙膏组成原料及重量百分比含量如下表1所示：
表1含面针提取物、柚皮苷、断血流提取物的中药护龈牙膏
二、含两面针提取物、柚皮苷、断血流提取物的中药护龈牙膏的止血效果
取健康新西兰兔每组30只，体重2.0～2.5kg，雌雄兼用。用3％戊巴比妥钠30mg/kg耳缘静脉缓慢注入，家兔麻醉后，仰卧固定于手术台上，腹部正中切口长约6～8cm，暴露并固定肝脏，用手术剪在肝左叶切去肝脏组织长约1cm，宽约1cm，高约1cm，创面呈喷射样出血。擦去喷出的血后，立即在创面局部敷涂待试物样品1g，纱布复盖，以200g砝码压迫1min，1min后移去砝码，观察止血效果，以不出血为止血，如仍出血，则加敷涂待试物样品后继续加压，每隔30s观察一次，记录止血时间，如超过3min仍然出血，则记为未能止血，止血时间记为＞3min。每次样品测试后，用37℃生理盐水洗去残留受试物，使创面重新出血，再测试下一受试物样品。每只兔子进行四次肝脏局部创面损伤止血测试实验，各受试物样品按拉丁方程序敷药，以减少肝脏局部创面损伤切口差异和用药顺序的影响。每个受试物样品总计进行20次兔肝脏局部创面损伤出血的止血实验。止血效果按倒数计分法(10/T)记为分值F，3min以上未能止血，F无分值记为0。结果见表2。
表2含两面针提取物、柚皮苷、断血流提取物的中药护龈牙膏对兔肝脏局部创面损伤出血的止血效果
注：与淀粉组比较：#P＜0.05,##P＜0.01,###P＜0.001,▲P＞0.05.
与牙膏空白基质组比较：*P＜0.05,**P＜0.01,***P＜0.001,△P＞0.05.
HY-1为实施例1、HY-2为实施例2、HY-3为实施例3、HY-4为实施例4、HY-5为实施例5。
实验结果表明，牙膏HY-3组、牙膏HY-4组、牙膏HY-5组和云南白药组均对兔肝脏局部创面损伤出血有明显的止血效果，具有明显的止血作用。
三、含两面针提取物、柚皮苷、断血流提取物的中药护龈牙膏的抑菌效果
龋病和牙周病是口腔最常见的两大类疾病，牙菌斑是引发这两种疾病的始动因素。引起龋齿的致病菌是变形链球菌，它通过黏附、产酸和耐酸等破坏牙体硬组织，从而导致龋病的发生；牙龈卟啉单胞菌是牙周疾病最重要的致病菌之一，是与牙周炎密切相关的红色复合体中的重要成员。
1、抑菌实验
(1)需氧菌抑菌实验：按照QBT 2738-2012《日化产品抗菌抑菌效果的评价方法》进行抑菌效果测试。在20℃-21℃的条件下，牙膏实验浓度为1:1，作用时间2min，试验菌种均为金黄色葡萄球菌(ATCC 6538)和白色念珠菌(ATCC10231)2种菌种；抑菌率≥50％时，标识为有抑菌效果，重复三次；抑菌率≥90％时，标识为有较强抑菌效果，重复三次。结果见表3、表4、表5。
(2)厌氧菌抑菌实验：采用生物生长曲线监测法进行抑菌效果测试。以牙膏中上述提取组合物有效含量值配制成细菌培养液，作为试验组；以不含上述提取组合物成分的细菌培养液作为对照组，35℃-37℃，厌氧条件下，每间隔1小时，利用紫外分光光度计测定一次OD值，代表测试细菌浓度及生长情况。其中，牙龈卟啉单胞菌(ATCC 33277)测定波长为450nm，变形链球菌(ATCC 25175)测定波长为600nm。样品组生物生长曲线低于对照组生物生长曲线，标识为在该浓度下对细菌生长起到抑制作用，具有抑菌效果。结果见表6。
2、采用实施例1、2、3进行试验，空白对照样不含上述提取组合物。
表3含两面针提取物、柚皮苷、断血流提取物的中药护龈牙膏的需氧抑菌试验结果(实施例1)
表4含两面针提取物、柚皮苷、断血流提取物的中药护龈牙膏的需氧抑菌试验结果(实施例2)
表5含两面针提取物、柚皮苷、断血流提取物的多重护龈牙膏的需氧抑菌试验结果(实施例3)
表6含两面针提取物、柚皮苷、断血流提取物的多重护龈牙膏的厌氧菌试验结果(实施例1、2、3)
抑菌试验结果表明，结合图1和图2所示，实施例1、实施例2对金黄色葡萄球菌、白色念珠菌的抑菌率大于70％，具有抑菌效果。实施例1中，所含提取组合物含量值，对牙龈卟啉单胞菌、变形链球菌的生物细胞生长曲线具有一定的抑制效果。
实施例3对金黄色葡萄球菌、白色念珠菌的抑菌率大于90％，具有较强的抑菌效果。实施例3中，所含提取组合物含量值，对牙龈卟啉单胞菌、变形链球菌的生物细胞生长曲线具有较强的抑制效果，能降低其繁殖速率及生长时间。
四、含两面针提取物、柚皮苷、断血流提取物的中药护龈牙膏的抗炎效果
选取体重18～22g健康昆明种♂小白鼠60只，随机均分7组，每组10只。空白对照组(生理盐水0.1ml/只)、牙膏空白基质组(牙膏空白基质组0.1g/只)、醋酸氟轻松乳膏组(醋酸氟轻松乳膏0.1g/只)、牙膏HY-1组、牙膏HY-2组、牙膏HY-3组、牙膏HY-4组、牙膏HY-5组。实验时各组每只动物右耳廓外两面均匀涂抹二甲苯0.1ml/只致炎，左耳不加处理作自身空白对照。涂抹二甲苯30min后，各组每只动物右耳廓内外两面分别均匀涂抹各组受试物样品0.1g/只。给予受试物1h后，小白鼠颈椎脱臼处死，剪下两耳廓，用生理盐水洗去右耳廓受试物样品，干棉球擦净，重叠两耳廓，用内径8mm的打孔器在同一部位凿取两圆耳片，分别在FA1004型电子分析天平上称重，右耳片重量减去左耳片重量为肿胀值，数据进行统计学t检验。结果见表6。
表6含两面针提取物、柚皮苷、断血流提取物的中药护龈牙膏对二甲苯所致小白鼠耳廓急性炎症肿胀的影响
注：与空白对照组比较：#P＜0.05,##P＜0.01,###P＜0.001,▲P＞0.05.
与牙膏空白基质组比较：*P＜0.05,**P＜0.01,,***P＜0.001,△P＞0.05.
HY-1为实施例1、HY-2为实施例2、HY-3为实施例3、HY-4为实施例4、HY-5为实施例5。
实验结果表明，牙膏HY-3组、牙膏HY-4组、牙膏HY-5组和醋酸氟轻松乳膏组均能使小白鼠急性炎症肿胀耳廓重量显著减轻，明显抑制二甲苯所致小白鼠耳廓急性炎症肿胀，对急性炎症具有明显的抗炎作用。牙膏HY-1组、HY-2组未见能明显减轻小白鼠急性炎症肿胀耳廓重量，未见明显抑制二甲苯致小白鼠耳廓急性炎症肿胀，对急性炎症未见明显的抗炎作用。
Claims (6)
1.一种含两面针提取物的中药护龈口腔护理用品，其特征在于：
口腔护理用品含有中药活性成分，中药活性成分由两面针提取物、柚皮苷和断血流提取物，按重量百分比计，两面针提取物含量为0.1~10%，柚皮苷含量为0.01%～5%，断血流提取物含量为0.01~8%。
2.根据权利要求1所述的中药护龈口腔护理用品，其特征在于：
两面针提取物含量为0.1~2.0%。
3.根据权利要求1所述的中药护龈口腔护理用品，其特征在于：
柚皮苷提取物含量为0.02~1.0%。
4.根据权利要求1所述的中药护龈口腔护理用品，其特征在于：
断血流提取物含量为0.05~5.0%。
5.根据权利要求1所述的中药护龈口腔护理用品，其特征在于：
所述两面针提取物、所述柚皮苷和所述断血流提取物均为乙醇提取物。
6.根据权利要求1所述的中药护龈口腔护理用品，其特征在于：
所述口腔护理用品为牙膏、漱口水、牙粉、口腔喷雾剂、口腔清洗剂或口香糖。
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| CN101244016A (zh) * | 2008-03-17 | 2008-08-20 | 柳州两面针股份有限公司 | 柚皮苷在制备口腔护理保健品中的应用 |
| CN106491454A (zh) * | 2016-12-15 | 2017-03-15 | 柳州两面针股份有限公司 | 中药牙膏 |
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| CN101244016A (zh) * | 2008-03-17 | 2008-08-20 | 柳州两面针股份有限公司 | 柚皮苷在制备口腔护理保健品中的应用 |
| CN106491454A (zh) * | 2016-12-15 | 2017-03-15 | 柳州两面针股份有限公司 | 中药牙膏 |
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