CN115006322B

CN115006322B - Formula and preparation method of multifunctional eye mask with instant and long-acting effects

Info

Publication number: CN115006322B
Application number: CN202210633927.3A
Authority: CN
Inventors: 裴运林; 聂艳峰; 王娟; 郭朝万; 蒲艳; 胡露
Original assignee: Guangdong Marubi Biological Technology Co Ltd
Current assignee: Guangdong Marubi Biological Technology Co Ltd
Priority date: 2022-06-06
Filing date: 2022-06-06
Publication date: 2023-09-19
Anticipated expiration: 2042-06-06
Also published as: CN115006322A

Abstract

The application relates to the field of cosmetics, in particular to a multifunctional eye mask with instant and long-acting functions, which comprises the following components: EX-wine lees extract MR, schizophyllan-G1, nicotinamide and erythritol; and a second component: haloxy PH MBAL, bioGenic CellLite-220, MOISTSHIELD HA, all human sources ^TM Collagen, SENS' FLOWER SD-SC, naturalSuperM@2- αGG-40, XEP-018 conopeptides. Immediate efficacy: the glossiness of the skin under the eyes tends to be improved; under-eye skin tends to whiten; the canthus and the wrinkles under the eyes have a tendency to improve; the volume of the eye bag is reduced; the moisture content of the skin stratum corneum under the eyes tends to be increased; long-acting efficacy: the glossiness of the skin under the eyes tends to be improved; skin under eyesHas a tendency to whiten; the canthus and the wrinkles under the eyes have a tendency to improve; the pouch volume is reduced.

Description

Formula and preparation method of multifunctional eye mask with instant and long-acting effects

Technical Field

The application relates to the technical field of cosmetics, in particular to a formula of an instant and long-acting multifunctional eye mask and a preparation method thereof.

Background

The eyes often have edema, stay up, black eyes, eye bags and other conditions, and have the problems of wrinkles, glossiness, water content loss of skin horny layer and the like.

Therefore, it is required to supplement water in a short time, eliminate fatigue, rapidly reduce edema and dark circles, and improve wrinkles, glossiness, moisture loss of skin horny layer, etc. for a long time.

However, there is a lack of an ophthalmic product in the art that can be used to provide both immediate and long lasting benefits and multiple efficacy.

Disclosure of Invention

The embodiment of the application aims to provide a formula and a preparation method of an instant and long-acting multifunctional eye mask.

In a first aspect, the present application provides a formulation for a multi-functional ophthalmic film that combines immediate and long-lasting efficacy, comprising a first component, a second component, and water;

the first component comprises:

EX-wine lees extract MR, schizophyllan-G1, nicotinamide and erythritol;

the second component comprises:

haloxy PH MBAL, bioGenic CellLite-220, MOISTSHIELD HA, all human sources ^TM Collagen, SENS' FLOWER SD-SC, naturalSuperM@2- αGG-40, XEP-018 conopeptides.

The application provides a multi-effect eye mask with instant and long-acting effects, which can realize the following instant effects:

the glossiness of the skin under the eyes tends to be improved after the eye mask is used; under-eye skin tends to whiten; the canthus and the wrinkles under the eyes have a tendency to improve; the volume of the eye bag is reduced; the moisture content of the skin stratum corneum under the eyes tends to be increased;

meanwhile, the following long-acting effects can be realized:

the glossiness of the skin under the eyes tends to be improved after the eye mask is used; under-eye skin tends to whiten; the canthus and the wrinkles under the eyes have a tendency to improve; the volume of the eye bag is reduced; there is a tendency for the moisture content of the stratum corneum of the skin beneath the eyes to rise.

The multifunctional eye mask with instant and long-acting functions has the advantages of mild formula and difficult allergy.

In other embodiments of the application, the first component further comprises:

glycerolyether-26, glycerol, low molecular sodium hyaluronate, xanthan gum;

the second component further comprises:

1.3-butanediol, 1, 2-hexanediol, p-hydroxyacetophenone, hydrogenated castor oil, bergamot fruit oil, and Salvia oil.

In other embodiments of the present application, an ophthalmic film comprises, in mass percent:

0.1 to 0.5 percent of EX-wine dreg extract MR, 0.1 to 0.5 percent of schizophyllan-G1, 1 to 5 percent of nicotinamide, 2 to 5 percent of erythritol, 0.1 to 2.5%Haloxyl PH MBAL, 0.1 to 0.5%BioGenic CellLite to 220, 0.5 to 2 percent of MOISTSHIELD HA, 0.1 to 2 percent of fully human ^TM Collagen, 0.1% -0.5%SENS'FLOWER SD-SC, 0.5% -2% NaturalSuperM@2-alpha GG-40, 1% -5% XEP-018 conopeptide, 1% -5% glycereth-26, 1% -5% glycerol, 0.1% -0.3% low molecular sodium hyaluronate, 0.2% -0.4% xanthan gum, 2% -5% 1.3-butanediol, 0.3% -0.6%1, 2-hexanediol, 0.3% -0.6% p-hydroxyacetophenone, 0.03% -0.05% hydrogenated castor oil, 0.001% -0.003% bergamot fruit oil, 0.001% -0.003% Salvia oil;

the balance being water.

In other embodiments of the present application, the ophthalmic membrane having both immediate and long-acting functions is a colorless transparent liquid;

in other embodiments of the application, EX-wine lees extract MR comprises the following components: rice fermentation product filtrate, butylene glycol, water, citric acid, sodium citrate, 1, 2-hexanediol, and p-hydroxyacetophenone.

In other embodiments of the present application, naturalSuperM@2-. Alpha.GG-40 comprises the following components: glucosyl glycerol, water, 1, 2-pentanediol.

In a second aspect, the present application provides a method for preparing an ophthalmic membrane having both immediate and long-acting functions,

adding water and a first component into a water phase pot, heating and preserving heat to obtain a first material;

adding the first material into an emulsifying pot, homogenizing, cooling, and then adding the second component.

In other embodiments of the application, the water and the first component are added into a water phase pot, and in the step of heating and preserving heat, the heating and preserving heat is carried out by heating to 80-85 ℃.

In other embodiments of the application, the first material is added to the emulsifying kettle, and in the step of homogenizing and cooling, the temperature is reduced to 44-46 ℃.

In other embodiments of the application, the first material is added into the emulsifying pot, and in the steps of homogenizing and cooling, the homogenizing speed is 1000 r/min-2000 r/min.

Drawings

In order to more clearly illustrate the technical solutions of the embodiments of the present application, the drawings that are needed in the embodiments will be briefly described below, it being understood that the following drawings only illustrate some embodiments of the present application and therefore should not be considered as limiting the scope, and other related drawings may be obtained according to these drawings without inventive effort for a person skilled in the art.

FIG. 1 shows the differential analysis of the skin gloss under the eyesn＝32)；

FIG. 2 shows an analysis of the color difference of the skin under the eyesn＝32)；

FIG. 3 shows the analysis of the differences between the red pigment (EI) value and the Melanin (MI) value of the skin under the eyesn＝32)；

FIG. 4 shows an analysis of the volume difference of wrinkles at the corner of the eye and under the eyen＝32)；

FIG. 5 shows an analysis of the area difference of wrinkles at the corner of the eye and under the eyen＝32)；

FIG. 6 shows an analysis of the area-to-area difference of wrinkles at the corners and under the eyesn＝32)；

FIG. 7 shows an instant analysis of the difference in the volume of the pouchn＝32)；

FIG. 8 shows the analysis of the moisture content difference of the stratum corneum under the eyes immediatelyn＝32)；

FIG. 9 shows the analysis of the difference in the gloss values of the skin under the eyes for a long period of timen＝30)；

FIG. 10 shows a long-lasting under-eye skin color differential analysisn＝30)；

FIG. 11 shows a long-acting analysis of the difference between the skin haematochrome value and the melanin value under the eyesn＝32)；

FIG. 12 shows a long-acting analysis of the volume difference between the canthus and the wrinkles under the eyesn＝30)；

FIG. 13 shows a long-acting analysis of the area difference between the canthus and the wrinkles under the eyesn＝30)；

FIG. 14 shows a graph showing the difference in the area of wrinkles at the corners and under the eyes of a long-acting eyen＝30)；

FIG. 15 shows a long-acting pouch volume differential analysisn＝30)；

FIG. 16 shows the analysis of the moisture content difference of the stratum corneum under the eyes for a long period of timen＝30)。

Detailed Description

For the purpose of making the objects, technical solutions and advantages of the embodiments of the present application more apparent, the technical solutions of the embodiments of the present application will be clearly and completely described below with reference to the accompanying drawings in the embodiments of the present application, and it is apparent that the described embodiments are some embodiments of the present application, but not all embodiments of the present application. The components of the embodiments of the present application generally described and illustrated in the figures herein may be arranged and designed in a wide variety of different configurations.

Accordingly, the following detailed description of the embodiments of the application is not intended to limit the scope of the application, as claimed, but is merely representative of selected embodiments of the application. All other embodiments, which can be made by those skilled in the art based on the embodiments of the application without making any inventive effort, are intended to be within the scope of the application.

Some embodiments of the present application provide a formulation for an ophthalmic film that combines immediate and long-acting functions, comprising a first component, a second component, and water.

Further, the first component includes: EX-wine lees extract MR, schizophyllan-G1, nicotinamide and erythritol.

Further, the EX-wine lees extract MR described above comprises the following components: rice fermentation product filtrate, butylene glycol, water, citric acid, sodium citrate, 1, 2-hexanediol, and p-hydroxyacetophenone.

Further, the schizophyllan-G1 comprises the following components: butanediol, dextran, p-hydroxyacetophenone, 1, 2-hexanediol, sodium citrate, citric acid and water.

Further, in some embodiments of the application, the nicotinamide described above may be obtained commercially, for example by commercially available nicotinamide Niacinamide PC from lupei.

Further, in some embodiments of the application, the above-described erythritol can be obtained commercially, e.g., by commercially available erythritol Zerose from Orthomson ^TM Erythritol Standard Granular。

Further, the second component includes: haloxy PH MBAL, bioGenic CellLite-220, MOISTSHIELD HA, all human sources ^TM Collagen, SENS' FLOWER SD-SC, naturalSuperM@2- αGG-40, XEP-018 conopeptides.

Further, the above Haloxyl PH MBAL includes the following components: water, glycerol, steareth-20, N-hydroxysuccinimide, 5, 7-dihydroxyflavone, palmitoyl tripeptide-1, palmitoyl tetrapeptide-7, phenoxyethanol, potassium sorbate, citric acid, and sodium citrate.

Further, the BioGenic CellLite-220 includes the following components: coffee cherries (COFFEA araica) seed extract, maltodextrin, cyclodextrin, xanthan gum.

Further, the MOISTSHIELD HA comprises the following components: water, xanthan gum, butylene glycol, dextrose, sodium hyaluronate, carrageenan (CHONDRUS CRISPUS) extract, hydroxypropyl trimethyl ammonium chloride hyaluronic acid, hydrolyzed hyaluronic acid, sodium hyaluronate, hyaluronic acid, sodium hyaluronate cross-linked polymer, hydrolyzed sodium hyaluronate, potassium hyaluronate, 1, 2-hexanediol, and caprylyl glycol.

Further, the above-mentioned fully human source ^TM The collagen comprises the following components: water, glycerol, sodium dihydrogen phosphate, disodium hydrogen phosphate, and soluble collagen.

Further, the SENS' FLOWER SD-SC comprises the following components: water, 1, 3-propanediol, CROCUS SATIVUS (CROCUS SATIVUS) flower extract.

Further, the NaturalSuperM@2- αGG-40 comprises the following components: glucosyl glycerol, water, 1, 2-pentanediol.

Further, the XEP-018 conopeptides comprise the following components: water, cysteine, serine, arginine/lysine polypeptides.

Further, in some embodiments of the present application, the first component further comprises: glycerolyether-26, glycerol, low molecular sodium hyaluronate, and xanthan gum.

Further, in some embodiments of the present application, the second component further comprises: 1.3-butanediol, 1, 2-hexanediol, p-hydroxyacetophenone, hydrogenated castor oil, bergamot fruit oil, and Salvia oil.

Further, in some embodiments of the present application, the ophthalmic film comprises, in mass percent:

0.1 to 0.5 percent of EX-wine dreg extract MR, 0.1 to 0.5 percent of schizophyllan-G1, 1 to 5 percent of nicotinamide, 2 to 5 percent of erythritol, 0.1 to 2.5%Haloxyl PH MBAL, 0.1 to 0.5%BioGenic CellLite to 220, 0.5 to 2 percent of MOISTSHIELD HA, 0.1 to 2 percent of fully human ^TM Collagen, 0.1-0.5%SENS'FLOWER SD-SC, 0.5-2% NaturalSuperM@2-alpha GG-40, 1-5%XEP-018 conopeptide, 1% -5% of glycereth-26, 1% -5% of glycerin, 0.1% -0.3% of low molecular sodium hyaluronate, 0.2% -0.4% of xanthan gum, 2% -5% of 1.3-butanediol, 0.3% -0.6% of 1, 2-hexanediol, 0.3% -0.6% of p-hydroxyacetophenone, 0.03% -0.05% of hydrogenated castor oil, 0.001% -0.003% of bergamot fruit oil and 0.001% -0.003% of salvia miltiorrhiza oil; the balance being water.

Further, in some embodiments of the present application, the ophthalmic membrane that combines both immediate and long-acting functions is a colorless transparent liquid.

The eye mask has the effects of instant and long-acting, and the formula is mild.

Some embodiments of the present application provide a method for preparing an ophthalmic membrane having both immediate and long-acting functions, which can be used to prepare an ophthalmic membrane having both immediate and long-acting functions provided in any of the foregoing embodiments.

Further, the preparation method comprises the following steps: adding water and a first component into a water phase pot, heating and preserving heat to obtain a first material;

Further, adding water and the first component into a water phase pot, and heating and preserving heat, wherein the heating and preserving heat is to 80-85 ℃ for preserving heat.

Illustratively, the above-mentioned heating and heat-preserving is performed by heating to 80 ℃, 81 ℃, 82 ℃, 83 ℃, 84 ℃, or 85 ℃.

Further, the first material is added into an emulsifying pot, and in the steps of homogenizing and cooling, the temperature is reduced to 44-46 ℃.

The above-described cooling is, for example, cooling to 44 ℃, 44.5 ℃, 45 ℃, 45.5 ℃ or 46 ℃.

Further, the first material is added into an emulsifying pot, and in the steps of homogenizing and cooling, the homogenizing speed is 1000 r/min-2000 r/min.

Exemplary speeds for such homogenization are 1000r/min, 1200r/min, 1500r/min, 1800r/min, or 1900r/min.

The preparation method of the eye mask with the instant and long-acting functions has the advantages of simple process, environmental protection and convenient use.

The features and capabilities of the present application are described in further detail below in connection with the examples.

Example 1

An eye mask with instant and long-acting functions is provided, and the formula composition is shown in table 1:

TABLE 1

The preparation method comprises the following steps:

adding water and the first component into a water phase pot, heating to 80-85 ℃ and preserving heat to obtain a first material;

adding the first material into an emulsifying pot, homogenizing and cooling to 44-46 ℃, wherein the homogenizing speed is 1000-2000 r/min; the second component is then added.

Example 2

An eye mask with instant and long-acting functions is provided, and the formula composition is shown in table 2:

TABLE 2

The preparation method is the same as in example 1.

Example 3

An eye mask with instant and long-acting functions is provided, and the formula composition is shown in table 3:

TABLE 3 Table 3

The preparation method is the same as in example 1.

The following test was conducted on the efficacy of the cosmetics in examples 1 to 3, including immediate effect and long-lasting effect.

1. Test index

(1) Under eye skin gloss;

(2) The individual type angle (individual type angle, ITA) of the skin under the eyes is characterized in that the three basic stimulus values (L, a and b) determined by the International Commission on illumination are adopted to quantify the skin color, and the individual type angle ITA value is calculated according to a formula to represent the whiteness of the skin;

(3) Undereye skin haematochrome content, melanin content;

(4) The volume of wrinkles, the area of wrinkles and the area of wrinkles at the corners of the eyes and under the eyes;

(5) Pouch volume;

(6) Moisture content of the stratum corneum of the skin under the eyes;

(7) Subjective evaluation of the subjects: and evaluating indexes such as product use effect, use feeling, use willingness and the like.

2. Detecting an environment

The test environment is the temperature of 20.5-21.8 ℃ and the humidity of 43.8-56.4% (RH), which meets the requirements of scheme design.

3. Instrument test results

After face cleaning for 30min, collecting facial images of the subjects by using a VISIA-CR, collecting wrinkle images of a measuring area of the subjects by using a PRIMOS-CR, determining a wrinkle target analysis position, and obtaining the volume values of wrinkles at the corners of eyes and under the eyes, the wrinkle area and the wrinkle area occupation ratio by using analysis software, wherein the volume of the eye bags is the same as that of the eyes; and detecting skin cuticle water content, skin glossiness, skin red pigment content, melanin content and skin color ITA value at the test position by using CM825, GL200, MX18 and CM-700d, wherein the measured values are all baseline values (T0), and then dispensing the product and guiding the use method. Subjects were asked to return to visit 0.5 hours (T0.5h) after using the product and to conduct the same index test. Subjects were given 1 time every 2 days, for 28 consecutive days, interviewed 7 days (D7) and 28 days (D28) for the product, and subjected to the same index test.

Testing of the same subject was accomplished using the same instrument, the same tester. The front and back of the test part should be kept consistent between the two test time points.

The measured values of each test area are counted, including numerical value, mean value, standard deviation, standard error, minimum value, median, maximum value and the like.

If the test data is normal analysis, adopting a t-test method to carry out statistical analysis; if the test data are in non-normal distribution, adopting a rank sum test method to carry out statistical analysis.

The statistical methods all use a two-tailed test with a test level α=0.05.

Data statistics were analyzed for variability using SPSS25.0 software. When significant differences in results occur, P < 0.05 and P < 0.01 are noted, respectively. * P < 0.05 means statistically significant, P < 0.01 means significantly different. If the index values of the test areas have statistical significance or significance difference, the product has the effect of improving wrinkles and preserving moisture.

4. Instant effect detection result

Under-eye skin gloss analysis

The results are shown in tables 1,2 and FIG. 1:

table 1 under-eye skin gloss value descriptive statistics (n=32)

TABLE 2 analysis of skin gloss values under eye differentialn＝32)

Time point	Mean ± standard deviation	P value (vs.T0)
			T0	3.68±1.91	/
T0.5h	5.80±2.08	<0.01

As can be seen from tables 1,2 and fig. 1, the under-eye skin gloss values show an increasing trend after the use of the product, compared to the pre-use baseline value (T0). The under-eye skin gloss value increased by 57.58% after 0.5 hours (T0.5 h) of application of the product, and was significantly different from the baseline value by t-test analysis, and was higher than the baseline value after application.

(two) under-eye skin color ITA-analysis

The results are shown in tables 3, 4 and fig. 2:

table 3 under-eye skin color ITA ° value descriptive statistics (n=32)

Time point	The number of people	Average value of	Standard deviation of	Standard error	Minimum value	Maximum value	Median of
								T0	32	18.75	6.95	1.23	6.70	36.46	18.84
T0.5h	32	21.28	6.15	1.09	5.43	37.94	21.41

TABLE 4 under-eye skin color ITA-value differential analysisn＝32)

Time point	Mean ± standard deviation	P value (vs.T0)
			T0	18.75±6.95	/
T0.5h	21.28±6.15	<0.01

As can be seen from tables 3, 4 and fig. 2, the under-eye skin color ITA's value showed a gradual upward trend after using the product, 13.51% after using the product for 0.5 hours (t0.5 h), and the difference from the baseline value T0 was significant by T-test analysis, and the under-eye skin color ITA's value increased after using the product for 0.5 hours (t0.5 h).

(III) analysis of under-eye skin haematochrome (EI) value and Melanin (MI) value

The results are shown in tables 5, 6 and FIG. 3:

table 5 descriptive statistics of skin haematochrome and melanin values under the eyes (n=32)

TABLE 6 skin under eye haematochrome and melanin differencesSex analysis [ ]n＝32)

As can be seen from tables 5, 6 and fig. 3, the under-eye skin red and melanin values tended to decrease by 7.56% and 5.82% after 0.5 hours of application of the product, respectively, as compared to the pre-application baseline value (T0). The paired T test analysis shows that the difference from the base line value T0 is significant, and the red pigment value and the melanin value of the skin under the eyes are reduced after the product is used.

(IV) analysis of the volume of the canthus and under-the-eye wrinkles

The results are shown in tables 7, 8 and fig. 4:

table 7 eye corner and under eye wrinkle volume descriptive statistics (mm) ³ ,n＝32)

TABLE 8 analysis of the volume variability of wrinkles at the canthus and under the eyes (mm) ³ ,n＝32)

As can be seen from table 7, table 8 and fig. 4: the canthus and under-the-eye wrinkle volume values all showed a decreasing trend after product use compared to the pre-use baseline value (T0). Wherein, the canthus wrinkle volume value was reduced by 4.71% after 0.5 hours (T0.5 h) of use of the product, compared to the pre-use baseline value (T0). The paired T test analysis shows that the difference from the baseline value T0 is significant, and the volume of the canthus wrinkles is reduced after the paired T test analysis is used.

The under-eye wrinkle volume value was 7.87% lower after 0.5 hours (T0.5) of use of the product, compared to the pre-use baseline value (T0). The difference from the baseline value T0 was statistically significant by paired T-test analysis.

Five canthus and under-eye wrinkle area analysis

The results are shown in Table 9, table 10 and FIG. 5:

table 9 descriptive statistics of canthus and under-the-eye wrinkle area (mm) ² ,n＝32)

TABLE 10 analysis of area differentiation (mm) of canthus and under-eye wrinkles ² ,n＝32)

As can be seen from table 9, table 10 and fig. 5: the canthus and under-the-eye wrinkle area values all showed a decreasing trend after product use compared to the pre-use baseline value (T0).

Wherein the canthus wrinkle area value was reduced by 9.23% after 0.5 hours of use of the product, as compared to the pre-use baseline value (T0). The difference between the product and the base line value T0 is obvious through T test analysis, and the area of wrinkles at the canthus is reduced after the product is used.

The under-eye wrinkle area value was reduced by 10.04% after 0.5 hours of application of the product, compared to the pre-application baseline value (T0). The difference from the baseline value T0 was significant by paired T-test analysis, with a decrease in the area of wrinkles under the eyes after use.

Analysis of the area ratio of canthus and under-eye wrinkles

The results are shown in Table 11, table 12 and FIG. 6:

table 11 canthus and under-the-eye wrinkle area ratio descriptive statistics (%, n=32)

Table 12 analysis of area ratio variability of canthus and under-eye wrinkles (%,n＝32)

as can be seen from table 11, table 12 and fig. 6: the canthus and under-the-eye wrinkle area ratio showed a decreasing trend after use of the product compared to the pre-use baseline value (T0)

Wherein, the canthus wrinkle area ratio was reduced by 9.19% after 0.5 hours (T0.5 h) of use of the product, compared to the pre-use baseline value (T0). The difference between the product and the baseline value T0 is significant through rank and test analysis, and the area occupied ratio of the wrinkles at the canthus is reduced after the product is used

The area of the under-eye wrinkles decreased by 10.07% after 0.5 hours of use, compared to the baseline value before use (T0). The difference from the baseline value T0 is significant after rank and test analysis, and the area of wrinkles under eyes is reduced after the product is used.

Seven pouch volume analysis

The results are shown in Table 13, table 14 and FIG. 7:

table 13 eye bag volume descriptive statistics (mm ³ ,n＝32)

Time point	The number of people	Average value of	Standard deviation of	Standard error	Minimum value	Maximum value	Median of
								T0	32	237.28	75.16	13.29	114.13	395.29	216.83
T0.5h	32	227.52	80.62	14.25	106.59	377.45	207.57a

Table 14 analysis of pouch volume variability (mm) ³ ,n＝32)

Time point	Mean ± standard deviation	P value (vs.T0)
			T0	237.28±75.16	/
T0.5h	227.52±80.62	<0.01

As can be seen from table 13, table 14 and fig. 7:

the pouch volume value showed a decreasing trend after product use compared to the pre-use baseline value (T0). The pouch volume value is reduced by 4.12% after 0.5 hours of use of the product, compared with the baseline value before use, and the pouch volume is reduced after 0.5 hours of use of the product due to the significance of the difference from the baseline value T0 by paired T test analysis.

(eight) analysis of moisture content of the stratum corneum of the skin under the eyes

The results are shown in FIG. 8:

as can be seen from fig. 8: the moisture content of the skin stratum corneum under the eyes showed an upward trend after the use of the product, compared to the baseline value before use. The moisture content of the stratum corneum of the skin under the eyes increased by 6.26% after 0.5 hours of using the product. The difference between T0.5 and the baseline value T0 was significant as analyzed by paired T test, and the moisture content of the skin stratum corneum under the eyes increased after 0.5 hours of using the product.

5. Conclusion of the instant test

The glossiness of the skin under the eyes tends to be improved after the use of the ophthalmic film products of examples 1 to 3; under-eye skin tends to whiten; the canthus and the wrinkles under the eyes have a tendency to improve; the volume of the eye bag is reduced; there is a tendency for the moisture content of the stratum corneum of the skin beneath the eyes to rise.

6. The long-acting effect is as follows: d7 and D28 day test effect

Under-eye skin gloss analysis

The results are shown in Table 15, table 16 and FIG. 9:

table 15 under-eye skin gloss value descriptive statistics (n=30)

Time point	The number of people	Average value of	Standard deviation of	Standard error	Minimum value	Maximum value	Median value
								D0	30	3.61	1.91	0.35	0.96	7.52	3.06
D7	30	4.42	1.82	0.33	0.91	8.23	4.04
								D28	30	6.60	1.75	0.32	3.31	10.57	6.58

TABLE 16 under-eye skin gloss value differential analysisn＝30)

Time point	Mean ± standard deviation	P value (vs.T0)
			D0	3.61±1.91	/
D7	4.42±1.82	<0.01
			D28	6.60±1.75	<0.01

As can be seen from tables 15, 16 and fig. 9, the under-eye skin gloss values showed an ascending trend after the use of the product, compared to the pre-use baseline value (D0). The under-eye skin gloss values increased by 22.44% and 82.83% after 7 days (D7) and 28 days (D28) of use of the product, and were significantly different from the baseline values as analyzed by t-test, and were higher than the baseline values after use.

(two) under-eye skin color ITA-analysis

The results are shown in Table 17, table 18 and FIG. 10:

table 17 under-eye skin gloss value descriptive statistics (n=30)

Time point	The number of people	Average value of	Standard deviation of	Standard error	Minimum value	Maximum value	Median value
								D0	30	19.06	6.84	1.25	6.70	36.46	18.84
D7	30	23.74	4.85	0.89	15.10	36.29	23.28
								D28	30	27.23	6.01	1.10	15.94	37.06	26.94

TABLE 18 analysis of skin gloss value differentiation under eyen＝30)

Time point	Mean ± standard deviation	P value (vs.T0)
			D0	19.06±6.84	/
D7	23.74±4.85	<0.01
			D28	27.23±6.01	<0.01

As can be seen from tables 17, 18 and fig. 10, the under-eye skin color ITA's value showed a gradual upward trend after the use of the product compared to the pre-use baseline value (D0), increased by 24.55% and 42.86% after the use of the product for 7 days (D7) and 28 days (D28), and was significantly different from the baseline value T0 by T-test analysis, and the under-eye skin color ITA's value was higher than the baseline value after the use of the product for 7 days (D7) and 28 days (D28).

The results are shown in Table 19, table 20 and FIG. 11:

table 19 under-eye skin haematochrome and melanin descriptive statistics (n=30)

TABLE 20 differential analysis of skin haematochrome and melanin values under eyesn＝30)

As can be seen from tables 19, 20 and fig. 11, the under-eye skin red pigment value slightly changed after 7 days (D7) of use of the product compared to the pre-use baseline value (D0), but the difference from the baseline value D0 was not statistically significant by t-test analysis; there was a trend to decrease by 12.12% after 28 days of use of the product (D28) compared to D0. The paired T test analysis shows that the difference from the baseline value T0 is significant, and the skin red pigment value under the eyes is lower than the baseline value after 28 days of using the product.

The under-eye skin red and melanin values tended to decrease by 3.47% and 5.18% after 7 days (D7) and 28 days (D28) of use of the product, respectively, as compared to the pre-use baseline value (D0). The paired T test analysis shows that the difference from the baseline value T0 has no statistical significance.

(IV) analysis of the volume of the canthus and under-the-eye wrinkles

The results are shown in Table 21, table 22 and FIG. 12:

table 21 volume descriptive statistics (mm) of canthus and under-the-eye wrinkles ³ ,n＝30)

Table 22 analysis of the volume variability (mm) of wrinkles at the canthus and under the eyes ³ ,n＝30)

As can be seen from table 21, table 22 and fig. 12: the canthus and under-the-eye wrinkle volume values all showed a decreasing trend after product use compared to the pre-use baseline value (D0).

Wherein the canthus wrinkle volume value was reduced by 2.89% after 7 days of use of the product (D7) as compared to the pre-use baseline value (D0). The difference from the baseline value T0 has no statistical significance through paired T test analysis; after 28 days of use (D28), 9.03% drop was observed, and the paired t-test analysis showed a significant difference from baseline D0, and the corner of the eye wrinkles volume was reduced after 28 days of use of the product.

The under-eye wrinkle volume value was reduced by 4.91% after 7 days (D7) of use of the product, compared to the pre-use baseline value (D0). The difference from the baseline value D0 has statistical significance after the rank and test analysis, and the volume of wrinkles under eyes is reduced after 7 days of using the product; after 28 days of use of the product (D28), a drop of 2.75% was obtained. The differences from the baseline value D0 were not statistically significant by rank sum test analysis.

Five canthus and under-eye wrinkle area analysis

The results are shown in Table 23, table 24 and FIG. 13:

table 23 descriptive statistics of canthus and under-the-eye wrinkle area (mm) ² ,n＝30)

TABLE 24 analysis of area variability (mm) of wrinkles under the eyes and corners of the eyes ² ,n＝30)

As can be seen from table 23, table 24 and fig. 13: the canthus and under-the-eye wrinkle area values all showed a decreasing trend after product use compared to the pre-use baseline value (D0).

Wherein the canthus wrinkle area value was reduced by 8.53% and 10.50% after 7 days and 28 days of use of the product, respectively, as compared to the pre-use baseline value (D0). The difference from the baseline value D0 is significant through t test analysis, and the area of the canthus wrinkles is reduced after the product is used.

The under-eye wrinkle area values were reduced by 7.42% and 6.48% after 7 days and 28 days of use of the product, respectively, as compared to the pre-use baseline value (D0). The difference from the baseline value T0 was significant by paired T-test analysis, with a decrease in the area of wrinkles under the eyes after use.

Analysis of the area ratio of canthus and under-eye wrinkles

The results are shown in Table 23, table 24 and FIG. 14:

table 25 canthus and under-the-eye wrinkle area ratio descriptive statistics (%, n=30)

TABLE 26 analysis of area differentiation (mm) of canthus and under-eye wrinkles ² ,n＝30)

As can be seen from table 25, table 26 and fig. 14: compared with the baseline value (D0) before use, the area ratio of the canthus and the wrinkles under the eyes shows a decreasing trend after the product is used

Wherein the canthus wrinkle area ratio was reduced by 6.82% and 9.15% after 7 days and 28 days of use of the product, respectively, as compared to the pre-use baseline value (D0). The paired t test analysis shows that the difference from the baseline value D0 is significant, and the area ratio of the wrinkles at the canthus after using the product is reduced

The area ratio of wrinkles under the eyes was reduced by 6.70% and 5.79% after 7 days and 28 days of application of the product, respectively, as compared to the baseline value before application (D0). The paired t test analysis shows that the difference from the baseline value D0 is significant, and the area of wrinkles under eyes is reduced after the paired t test analysis is used.

Seven pouch volume analysis

The results are shown in Table 27, table 28 and FIG. 15:

table 27 eye bag volume descriptive statistics (mm ³ ,n＝30)

Group of	The number of people	Average value of	Standard deviation of	Standard error	Minimum value	Maximum value	Median value
								D0	30	133.88	45.13	8.24	55.37	211.05	136.27
D7	30	129.93	45.47	8.30	48.71	203.56	138.12
								D28	30	126.94	43.96	8.03	51.73	199.62	125.10

Table 28 analysis of pouch volume variability (mm) ³ ,n＝30)

Group of	Mean ± standard deviation	P value (vs. D0)
			D0	133.88±45.13	/
D7	129.93±45.47	ns
			D28	126.94±43.96	<0.05

The results are shown in Table 27, table 28 and FIG. 15: the pouch volume value showed a decreasing trend after use of the product, compared to the pre-use baseline value (D0). The pouch volume value is reduced by 2.95% after 7 days of using the product compared with the baseline value before using, and has no statistical significance with the baseline value D0 through paired t test analysis; after 28 days of use of the product, a 5.18% drop was observed, which was statistically significant with baseline D0 as determined by paired t-test analysis, and the pouch volume was reduced after 28 days of use of the product.

The results are shown in Table 29, table 30 and FIG. 16:

table 29 descriptive statistics of skin stratum corneum moisture content under eye (c.u., n=30)

Table 30 analysis of moisture content of the stratum corneum under the eyes (c.u.,n＝30)

group of	Mean ± standard deviation	P value (vs. D0)
			D0	74.38±9.34	/
D7	76.50±7.20	ns
			D28	78.13±6.62	ns

As can be seen from tables 29, 30 and fig. 16, the moisture content of the skin stratum corneum under the eyes showed an ascending trend after the use of the product, compared to the baseline value before the use. The moisture content of the skin stratum corneum under the eyes increased by 2.85% and 5.04% after 7 days and 28 days of application of the product, respectively. The paired t-test and rank sum test analysis did not have statistical significance with baseline value D0.

7. Conclusion of the long-term test

8. Sensitive muscle test

The test was performed according to cosmetic safety technical Specification 2015.

The test results are shown in the following table:

as can be seen from the above table, after 4 weeks of using the eye masks of examples 1 to 3 of the present application, the skin response score of each of the 33 subjects was 0, and no adverse reaction and adverse event occurred. Therefore, the eye mask disclosed by the application is mild in formula and not easy to allergic.

The above description is only of the preferred embodiments of the present application and is not intended to limit the present application, but various modifications and variations can be made to the present application by those skilled in the art. Any modification, equivalent replacement, improvement, etc. made within the spirit and principle of the present application should be included in the protection scope of the present application.

Claims

1. An instant and long-acting multi-functional eye mask is characterized in that,

the eye mask comprises the following components in percentage by mass:

0.1% -0.5% EX-wine meal extract MR, 0.1% -0.5% schizophyllan-G1, 1% -5% nicotinamide, 2% -5% erythritol, 0.1% -2.5%Haloxyl PH MBAL, 0.1% -0.5%BioGenic CellLite-220, 0.5% -2% MOISTSHIELD HA, 0.1% -2% fully human ™ collagen, 0.1% -0.5%SENS'FLOWER SD-SC, 0.5% -2% NaturalSuperM@2-alpha GG-40, 1% -5% XEP-018 conopeptide, 1% -5% glycerol polyether-26, 1% -5% glycerol, 0.1% -0.3% low molecular sodium hyaluronate, 0.2% -0.4% xanthan gum, 2% -5% 1.3-butanediol, 0.3% -0.6%1, 2-hexanediol, 0.3% -0.6% p-hydroxy acetophenone, 0.03% -0.05% hydrogenated castor oil, 0.001% -0.003% citrus oil, and 0.001% -0.001% citrus oil;

the balance being water;

the EX-wine dreg extract MR comprises the following components: rice fermentation product filtrate, butanediol, water, citric acid, sodium citrate, 1, 2-hexanediol, and p-hydroxyacetophenone;

the schizophyllan-G1 comprises the following components: butanediol, dextran, p-hydroxyacetophenone, 1, 2-hexanediol, sodium citrate, citric acid, and water;

the Hall PH MBAL comprises the following components: water, glycerol, steareth-20, N-hydroxysuccinimide, 5, 7-dihydroxyflavone, palmitoyl tripeptide-1, palmitoyl tetrapeptide-7, phenoxyethanol, potassium sorbate, citric acid, sodium citrate;

the BioGenic CellLite-220 comprises the following components: an extract of coffee cherries, maltodextrin, cyclodextrin, xanthan gum;

the MOISTSHIELD HA comprises the following components: water, xanthan gum, butylene glycol, glucose, sodium hyaluronate, carrageenan extract, hydroxypropyl trimethylammonium chloride hyaluronic acid, hydrolyzed hyaluronic acid, acetylated sodium hyaluronate, hyaluronic acid, sodium hyaluronate cross-linked polymer, hydrolyzed sodium hyaluronate, potassium hyaluronate, 1, 2-hexanediol, octanediol;

the fully human ™ collagen comprises the following components: water, glycerol, sodium dihydrogen phosphate, disodium hydrogen phosphate, and soluble collagen;

the SENS' FLOWER SD-SC comprises the following components: water, 1, 3-propanediol, CROCUS SATIVUS (CROCUS SATIVUS) flower extract;

the NaturalSuperM@2-alpha GG-40 comprises the following components: glucosyl glycerol, water, 1, 2-pentanediol;

the XEP-018 conopeptide comprises the following components: water, cysteine, serine, arginine/lysine polypeptides.

2. The multi-functional ophthalmic membrane of claim 1 wherein the multi-functional ophthalmic membrane is a combination of immediate and extended-use ophthalmic membrane,

the multifunctional eye mask with instant and long-acting functions is colorless transparent liquid.