CN114680336A - Drop containing fructus cannabis unsaturated fatty acid and preparation method thereof - Google Patents

Abstract

The invention provides a fructus cannabis unsaturated fatty acid-containing drop, which comprises DHA algae oil, fructus cannabis unsaturated fatty acid, soybean phospholipid, a flavoring agent and fructus cannabis oil. After the DHA algae oil, the fructus cannabis unsaturated fatty acid, the soybean phospholipid and the fructus cannabis oil are combined in a reasonable proportion, the nutrition component proportion is reasonable, the bioavailability of each nutrition component is high, the in-vivo bioavailability of the DHA can be particularly obviously improved, and the DHA-containing health food has various effects of assisting in enhancing memory, preventing senile dementia, reducing blood fat and the like. The fructus cannabis-containing unsaturated fat drop is simple in preparation method, stable in product and capable of being stored for a long time.

Description

A kind of drops containing hemp seed unsaturated fatty acid and preparation method thereof

technical field

The invention relates to the field of food, in particular to a hemp seed unsaturated fatty acid-containing drop and a preparation method thereof.

Background technique

With the progress of society, the improvement of people's living standards and the improvement of medical security, the average life expectancy of my country's population has increased significantly, and the proportion of the elderly in the total population has continued to rise. The huge elderly population requires everyone to pay attention to the physical health of the elderly and improve the ability of the elderly to self-care and prevent diseases. Scientific research has found that the aging speed of brain cells begins to accelerate after the age of 60, the memory decline and the incidence of Alzheimer's disease (senile dementia) increase. The symptoms of Alzheimer's patients are manifested as cognitive impairment and mental disorders, and eventually they cannot take care of themselves, which will not only seriously affect the quality of life of the patients, but also bring a heavy burden to the patient's family and society. So far, Alzheimer's disease is incurable. The conventional drugs currently on the market can only relieve the symptoms of Alzheimer's disease, and the efficacy of the drugs is very short, which cannot control the development of the disease. There is no drug that can permanently stop the brain atrophy of patients. Therefore, it is of great significance to develop a food that can slow down the memory loss of the elderly, improve the memory of the elderly, and prevent Alzheimer's disease.

Hemp seed is a very good raw material for oil processing and food. Omega-3 unsaturated fatty acids in hemp seeds are a group of polyunsaturated fatty acids, which help to strengthen the brain, keep blood vessels open, and prevent the occurrence of cardiovascular and cerebrovascular diseases such as thrombosis or blood lipids. Among them, α-linolenic acid In the human body, it can be converted into docosahexaenoic acid (DHA), which maintains the operation of brain nerves, and provides sufficient nutrients to the brain. Lack of alpha-linolenic acid in the human body can easily lead to insufficient intake of Omega-3 unsaturated fatty acids and cause brain atrophy. For the elderly, lack of alpha-linolenic acid will increase the risk of Alzheimer's.

Taking Omega-3 can enhance learning ability, memory and concentration. Since Omega-3 unsaturated fatty acids cannot be synthesized by themselves in the body, Omega-3 unsaturated fatty acids can only be taken in from food, and a small amount of Omega-3 unsaturated fatty acids contained in daily food cannot meet the needs of the human body, so it is necessary to pass External supplementation of these essential unsaturated fatty acids improves human health.

At present, commercially available products rich in Omega-3 unsaturated fatty acids are mainly in the form of capsules, such as DHA soft capsules, fish oil soft capsules, krill oil capsules, etc. However, the storage period of the existing products is not long, and the capsules Omega-3 unsaturated fatty acid-rich oil has poor solubility, which is not conducive to human absorption, and its bioavailability is not high, which affects the efficacy of the product.

Therefore, there is a need to develop an unsaturated fatty acid product with a long shelf life and high bioavailability.

SUMMARY OF THE INVENTION

Aiming at the deficiencies of the prior art, an object of the present invention is to provide a kind of drops containing hemp seed unsaturated fatty acid, the ratio of each nutritional component of the drops is reasonable, the bioavailability in vivo is high, and the quality is stable and the storage period is long. , has multiple functions such as assisting memory enhancement, preventing senile dementia, lowering blood lipids, etc. Another object of the present invention is to provide a preparation method of the drops, which has the advantages of simple operation and suitable for large-scale production.

On the one hand, the present invention provides the hemp seed unsaturated fatty acid-containing drops, comprising the following components by mass percentage:

DHA algal oil: 5-25%;

Hemp seed unsaturated fatty acids: 5-65%;

Soy lecithin: 3-15%;

Flavoring agent: 0.1-0.8%;

Hemp seed oil: balance.

In a preferred embodiment, the hemp seed unsaturated fatty acid-containing drops of the present invention comprise the following components in mass percentage:

DHA algal oil: 10-20%;

Hemp seed unsaturated fatty acids: 10-60%;

Soy lecithin: 5-10%;

Flavoring agent: 0.3-0.5%;

Hemp seed oil: balance.

DHA algae oil is the general term for all the oily substances in seaweed. It is mostly a light yellow liquid with a slightly fishy smell at room temperature. The main active ingredients are EPA and DHA, etc. Improve blood clots and prevent cardiovascular disease. DHA is an indispensable material basis for the formation, development and movement of brain cells. The level of DHA in the body directly affects the proliferation of brain cells, nerve conduction, and the growth and development of synapses. Human memory and thinking function depend on DHA to maintain and improve. DHA supplementation can promote the full development of brain cells, delay mental decline, forgetfulness and prevent Alzheimer's disease.

Hemp seed unsaturated fatty acids are high-grade health-care edible oils, with unsaturated fatty acid content ≥92%, linoleic acid ω-6 ≥56%, linolenic acid ω-3 ≥14%, rich in natural VA, VD and other nutrients. Hemp seed unsaturated fatty acids have the effect of strengthening the brain and nourishing the brain. Hemp seed unsaturated fatty acids do not contain cholesterol, which can effectively prevent the occurrence of Alzheimer's disease. It purifies the blood and supplies fresh blood to the brain. Alpha-linolenic acid is a polyunsaturated fatty acid with three double bonds. It is an omega-3 essential fatty acid. It is used to improve intelligence, antithrombosis, and liver protection. It is one of the essential nutrients for people.

From the perspective of physiological activity, soybean phospholipid is obtained by introducing unsaturated fatty acids such as medium carbon chain oil, conjugated linoleic acid, phytosterol or n-3 series polyunsaturated fatty acids into phospholipid molecules through transesterification reaction. Phospholipids not only maintain the good emulsifying properties of phospholipids, but also greatly improve the nutritional value of phospholipids. When used together with DHA algal oil and hemp seed unsaturated fatty acids, they have a better effect of promoting absorption. Soybean phospholipids are similar to the key or carrier of the blood-brain barrier, carrying nutrients such as DHA in DHA algal oil and linolenic acid omega-3 in unsaturated fatty acids of hemp seeds into the brain through the blood-brain barrier, supplementing nutrition to the human brain, Improve memory and prevent Alzheimer's.

In the hemp seed unsaturated fatty acid-containing drops of the present invention, the proportions of nutrients are reasonable and meet international requirements. Improve the bioavailability of DHA in vivo. The reasonable combination of the above nutrients can help improve memory, prevent senile dementia, and lower blood lipids.

In a preferred embodiment, in the hemp seed unsaturated fatty acid-containing drops, the flavoring agent is a mixture of ethyl maltol and food flavor, wherein the quality of ethyl maltol and food flavor The ratio is 2:3.

The ethyl maltol and food essence in the hemp seed unsaturated fatty acid-containing drops of the present invention diffuse into the mouth, thereby completely covering the bad smell of the raw material components in the drops, making the drops easier to eat.

In a preferred embodiment, the hemp seed unsaturated fatty acid-containing drops further comprise 0.1%-1.5% by mass of antioxidants, preferably 0.5%-1% by mass of antioxidants.

In a preferred embodiment, in the hemp seed unsaturated fatty acid-containing drops, the antioxidant may be vitamin C, vitamin E, or a combination thereof.

In a preferred embodiment, the particle size of the hemp seed unsaturated fatty acid-containing drops of the present invention is 350-500 nm, preferably 400-450 nm.

The drop of the present invention is a liquid preparation, which is a heterogeneous liquid dispersion system formed by multiphase mutual dispersion. The particle size of the drop of the present invention is 350-500 nm, preferably 400-450 nm, so that the drop is more stable , the shelf life is longer.

On the other hand, the invention provides the preparation method of the drop containing hemp seed unsaturated fatty acid, comprises the following steps:

1) DHA algal oil, hemp seed unsaturated fatty acid, soybean lecithin, flavoring agent and hemp seed oil are mixed evenly to make a mixed solution;

2) Into the prepared mixed solution, nitrogen protection was introduced, and the material was discharged at 20°C;

3) Sterilization, vacuum defoaming and filling to obtain hemp seed unsaturated fatty acid-containing drops.

In a preferred embodiment, in the preparation method of the hemp seed unsaturated fatty acid-containing drops of the present invention, an antioxidant is also added in step 2), wherein the antioxidant can be vitamin C, vitamin E or a combination thereof.

The method adopts the method of step-by-step mixing, and the raw materials are sequentially and gradually added into the mixing system to make it fully dispersed, and the obtained drops have stable dispersion, and nitrogen filling protection improves the stability and prolongs the storage period.

To sum up, compared with the prior art, the hemp seed unsaturated fatty acid-containing drops of the present invention and the preparation method of the drops have the following beneficial effects:

(1) the drops of the present invention containing hemp seed unsaturated fatty acid are combined by DHA algae oil, hemp seed unsaturated fatty acid, soybean lecithin, hemp oil in reasonable proportion, the nutrient distribution ratio is reasonable, and the nutrient bioavailability is high , especially can significantly improve the in vivo bioavailability of DHA, and has multiple effects such as assisting memory enhancement, preventing senile dementia, and lowering blood lipids; the drops are protected by nitrogen gas, and the particle diameter of the drops of the present invention is 350- 500nm, preferably between 400-450nm, makes the drops more stable and has a longer shelf life, which can be extended to 2 years. Therefore, the hemp seed unsaturated fatty acid-containing drops of the present invention have the advantage of long storage period.

(2) The preparation method of the drops of the present invention has the advantages of simple operation and suitable for large-scale production.

Detailed ways

In order to better understand the content of the present invention, the following is further described in conjunction with the embodiments, but the content of the present invention is not limited to these embodiments.

Hereinafter, unless otherwise specified, the reagents used in the examples of the present invention are conventional reagents in the art. The flavoring agent is a mixture of ethyl maltol and food essence in a mass ratio of 2:3. The selected DHA algal oil is the raw material with a DHA content of 75%, and the unsaturated fatty acid of hemp seed is a raw material with a α-linolenic acid content of 55%.

Embodiment 1: the preparation of the drops containing hemp seed unsaturated fatty acid

The components and their corresponding mass percentages are shown in Table 1, and are prepared by the following steps:

1) DHA algal oil, hemp seed unsaturated fatty acid, soybean lecithin, flavoring agent and hemp seed oil are mixed evenly to make a mixed solution;

2) Into the prepared mixed solution, nitrogen protection was introduced, and the material was discharged at 20°C;

3) Sterilization, vacuum defoaming and filling to obtain hemp seed unsaturated fatty acid-containing drops.

Example 2 to Example 6: Preparation of hemp seed unsaturated fatty acid-containing drops

With the same steps as in Example 1, according to the mass percentage of each component in Example 2 to Example 6 in Table 1, the hemp seed unsaturated fatty acid-containing drops were prepared.

Table 1 Each component and its mass percentage in Examples 1 to 6 (total mass 100g)

Embodiment 7: the preparation of the drops containing hemp seed unsaturated fatty acid

The components and their contents are the same as in Example 1, except that they are prepared through the following steps:

1) DHA algal oil, hemp seed unsaturated fatty acid, soybean lecithin, flavoring agent and hemp seed oil are mixed evenly to make a mixed solution;

2) Discharge the prepared mixed solution at 20°C;

3) Sterilization and filling to obtain hemp seed unsaturated fatty acid-containing drops.

Embodiment 8: the preparation of the drops containing hemp seed unsaturated fatty acid

The mass percentages of DHA algae oil, hemp seed unsaturated fatty acids, soybean lecithin and the flavoring agent are the same as in Example 1, and the difference from Example 1 is that it also contains 0.1% by mass of antioxidant vitamin C, and the remainder For hemp seed oil. The preparation method is as follows:

1) DHA algal oil, hemp seed unsaturated fatty acid, soybean lecithin, flavoring agent and hemp seed oil are mixed evenly to make a mixed solution;

2) To the prepared mixed solution, add antioxidant, mix well, introduce nitrogen protection, and discharge at 20°C;

3) Sterilization, vacuum defoaming and filling to obtain hemp seed unsaturated fatty acid-containing drops.

Embodiment 9: the preparation of the drops containing hemp seed unsaturated fatty acid

The mass percentages of DHA algal oil, hemp seed unsaturated fatty acids, soybean lecithin and the flavoring agent are the same as in Example 1, and the difference from Example 1 is that it also contains 1% by mass of antioxidant vitamin E, and the remainder For hemp seed oil. The preparation method is the same as in Example 8.

Embodiment 10: the preparation of the drops containing hemp seed unsaturated fatty acid

The mass percentages of DHA algae oil, hemp seed unsaturated fatty acids, soybean lecithin and flavoring agent are the same as in Example 1, and the difference from Example 1 is that it also contains 0.5% mass percentage of antioxidants, which are vitamins A mixture of C and vitamin E in a mass ratio of 1:1, and the balance is hemp seed oil. The preparation method is the same as in Example 8.

In the following Comparative Examples 1 to 3, the content of the replacement component is the same as that of the replaced component in Example 1, and the other components and their contents are the same as those of Example 1.

Comparative Example 1: Preparation of Oral Drops

The preparation was carried out according to the same steps as in Example 1, except that the soybean lecithin in Example 1 was replaced by monoglyceride.

Comparative Example 2: Preparation of Oral Drops

Prepare according to the same steps as Example 1, and the difference from Example 1 is that olive oil is used to replace the hemp seed oil in Example 1.

Comparative Example 3: Preparation of Oral Drops

Prepare according to the same steps as Example 1, and the difference from Example 1 is that olive oil is used to replace the unsaturated fatty acid of hemp seed in Example 1.

Comparative Example 4: Preparation of Oral Drops

Prepare according to the same steps as Example 1, and the difference from Example 1 is that the addition of hemp seed unsaturated fatty acid is 70%, and the additions of DHA algal oil, soybean lecithin, and flavoring agent are the same as in Example 1. Make up the remaining amount with hemp seed oil.

Comparative Example 5: Preparation of Oral Drops

Prepare according to the same steps as Example 1. The difference from Example 1 is that the addition of DHA algae oil is 30%, and the additions of hemp seed unsaturated fatty acid, soybean lecithin, and flavoring agent are the same as those in Example 1. 1. Make up the remaining amount with hemp seed oil.

Experimental example

Bioavailability Test:

A total of 160 male Wistar rats (provided by the Laboratory Animal Center, Department of Medicine, Peking University), weighing 260-280 g, were selected and divided into 16 groups. The drops in Examples 1-10 and Comparative Examples 1-6 were gavaged respectively in rats, and blood was collected from the orbital venous plexus after administration for 0.25h, 0.5h, 1h, 2h, 4h, 6h, 8h, 12h and 24h 0.5mL was collected in a heparinized test tube, incubated at 37°C in a hemp seed oil bath for 30min, centrifuged at a low temperature of 5000r/min for 10min, and the plasma was separated to take the supernatant, which was stored at -20°C until its drug concentration was determined. The measured data of DHA blood concentration-time curve are included in Table 2 below after DAS pharmacokinetic calculation.

Table 2 DHA bioavailability test results

It can be seen from the test data in Table 2 that the components in the drops interact with each other, and the replacement of components and the change of component distribution ratios all have an impact on the absorption of each component. The drops with specific proportioning components protected by the present application can improve the bioavailability of DHA. Specifically, the bioavailability of DHA to the drops in rats in Examples 1-10 is significantly higher than the bioavailability of DHA for the drops in rats in Comparative Examples 1-5, especially Examples 9-10 are the best implementations For example, its DHA bioavailability is the highest.

The above are only the preferred embodiments of the present invention, and the protection scope of the present invention is not limited to the above-mentioned embodiments, and all technical solutions under the idea of the present invention belong to the protection scope of the present invention. It should be pointed out that for those skilled in the art, some improvements and modifications without departing from the principle of the present invention should also be regarded as the protection scope of the present invention.

Claims (8)

1. A drop containing fructus cannabis unsaturated fatty acid comprises the following components in percentage by mass:

DHA algal oil: 5 to 25 percent;

fructus cannabis unsaturated fatty acids: 5 to 65 percent;

soybean lecithin: 3 to 15 percent;

flavoring agent: 0.1 to 0.8 percent;

hemp seed oil: and (4) the balance.

2. A drop containing fructus cannabis unsaturated fatty acid as claimed in claim 1, comprising the following components in mass percent:

DHA algal oil: 10 to 20 percent;

fructus cannabis unsaturated fatty acids: 10 to 60 percent;

soybean lecithin: 5 to 10 percent;

flavoring agent: 0.3 to 0.5 percent;

hemp seed oil: and (4) the balance.

3. Drops containing edestin fatty acids according to claim 1 or 2, wherein the flavouring is a mixture of ethyl maltol and food essence, wherein the mass ratio of ethyl maltol to food essence is 2: 3.

4. Drops containing edestin fatty acids according to claim 1 or 2, wherein they further comprise an antioxidant in a percentage by mass ranging from 0.1% to 1.5%, preferably from 0.5% to 1%.

5. Drops containing edestan unsaturated fatty acids according to claim 4, wherein said antioxidant is vitamin C, vitamin E or a combination thereof.

6. Drops containing fructus cannabis unsaturated fatty acid according to claim 1 or 2, wherein the particle size of the drops containing fructus cannabis unsaturated fatty acid is 350-500nm, preferably 400-450 nm.

7. A method for preparing drop containing fructus Cannabis unsaturated fatty acid comprises the following steps:

1) uniformly mixing DHA algae oil, fructus Cannabis unsaturated fatty acid, soybean phospholipid, correctant and fructus Cannabis oil to obtain mixed solution;

2) introducing nitrogen into the prepared mixed solution for protection, and discharging at the temperature of 20 ℃;

3) sterilizing, vacuum defoaming, and packaging to obtain drop containing fructus Cannabis unsaturated fatty acid.

8. The method according to claim 7, wherein an antioxidant is further added in step 2), wherein the antioxidant is vitamin C, vitamin E or a combination thereof.