CN114306506A - Traditional Chinese medicine compound composition with effect of enhancing organism immunity and preparation method and application thereof - Google Patents

Abstract

The invention discloses a traditional Chinese medicine compound composition with an immunity enhancing effect and a preparation method thereof, wherein the composition is prepared from 10-40 parts of astragalus membranaceus, 5-20 parts of wolfberry fruits, 5-20 parts of radix ophiopogonis, 1-15 parts of schisandra chinensis, 5-20 parts of poria cocos, 2-15 parts of angelica sinensis, 2-20 parts of sea buckthorns and 5-20 parts of chrysanthemum morifolium. According to the theory of traditional Chinese medicine and pharmacy, the eight medicines are selected for compatibility and use in combination with the characteristics of human body functions and the like, so that the whole formula has the effects of tonifying qi and blood, invigorating spleen and nourishing liver, regulating yin and yang, strengthening body resistance and reinforcing vitality, and enhancing the immunity of the human body, has mild effect, and is particularly suitable for the elderly or people with low immunity. In addition, the raw materials of the traditional Chinese medicine are both medicinal and edible products, and are safe and effective.

Description

A traditional Chinese medicine compound composition with the effect of enhancing immunity and its preparation method and application

technical field

The invention relates to a traditional Chinese medicine composition, in particular to a traditional Chinese medicine compound composition with the effect of enhancing immunity and its application.

Background technique

Immunity refers to the body's immune system to identify "self-interest" and "self-harm" components, and reject the physiological function of "self-harm". TCM believes that the occurrence and development of immune diseases are mainly related to congenital and acquired factors. Congenital factors include insufficient congenital endowments, and acquired factors include exogenous six evils, endogenous five evils, imbalance of ying and wei, disharmony of qi and blood, and dysfunction of viscera. closely related. In today's society, the pace of life is accelerating, the pressure of competition is increasing, coupled with the irregular diet and rest, etc., the body is in an unbalanced sub-health state, the immunity is reduced, and the incidence of other diseases is increased. It is difficult for the body to recover, and the immunity declines again and again, forming a vicious circle.

Most of the western medicines that can improve immunity on the market are aimed at certain immune diseases and have obvious side effects. Most of the health foods that can improve immunity are nutrition bars, biscuits, gummies, solid drinks, wine tea and related products, dairy products. , instant food and other forms appear, or the price is high or the effect is not significant, etc. In addition, the current epidemic prevention and control is becoming normal, more and more people are gradually turning their attention to enhancing physical health and improving immunity. become a major prevention trend.

Most Chinese herbal medicines have little or no toxic side effects. People attach great importance to food safety issues, and have formed the concept of "green, pollution-free" consumption. Therefore, the research and development of green and high-efficiency traditional Chinese medicine immune enhancers is an urgent requirement of the development of the times, and it is also an inevitable development trend, which has very important practical significance. As an integral part of traditional Chinese medicine, traditional Chinese medicine plays an important role in regulating the immunity of the body. According to the theory of traditional Chinese medicine, various traditional Chinese medicines in traditional Chinese medicine prescriptions can act on the body individually or collaboratively at multiple levels, multiple pathways, and multiple targets, identify themselves, and eliminate non-self components, so as to maintain the stability of the body's internal environment and improve the body's immunity. And most traditional Chinese medicines have low toxicity and side effects, long-lasting curative effect, safety and therapeutic effect can be guaranteed.

SUMMARY OF THE INVENTION

Purpose of the invention: The purpose of the present invention is to overcome the deficiencies of the above-mentioned technologies, and to provide a kind of raw material that adopts the same source of medicine and food. thing.

Technical scheme: in order to achieve the above purpose, the technical scheme adopted in the present invention is:

A traditional Chinese medicine compound composition with enhanced immunity, which is made from the following raw materials in parts by weight:

10-40 servings of Astragalus, 5-20 servings of Lycium barbarum, 5-20 servings of Ophiopogon japonicus, 1-15 servings of Schisandra, 5-20 servings of Poria, 2-15 servings of Angelica, 2-20 servings of sea buckthorn, 5-20 servings of Hangbaiju .

As a preferred scheme, the above-mentioned Chinese medicine compound composition with the effect of enhancing immunity is made from the following raw materials in parts by weight:

20-30 parts of Astragalus, 10-15 parts of Lycium barbarum, 10-15 parts of Ophiopogon japonicus, 5-10 parts of Schisandra, 10-15 parts of Poria, 4-10 parts of Angelica, 7-15 parts of sea buckthorn, 10-15 parts of Chrysanthemum .

As a preferred scheme, the above-mentioned Chinese medicine compound composition for enhancing immunity, the composition is made from the following raw materials by weight:

40 parts of Astragalus, 20 parts of Lycium barbarum, 20 parts of Ophiopogon japonicus, 10 parts of Schisandra, 20 parts of Poria, 8 parts of Angelica, 14 parts of sea buckthorn, 20 parts of Hangbai chrysanthemum.

As a preferred scheme, the above-mentioned Chinese medicine compound composition for enhancing immunity, the composition is made from the following raw materials by weight:

20 parts of Astragalus, 10 parts of Lycium barbarum, 10 parts of Ophiopogon japonicus, 5 parts of Schisandra, 10 parts of Poria, 4 parts of Angelica, 7 parts of sea buckthorn, 10 parts of Hangbai chrysanthemum.

As a preferred scheme, the above-mentioned Chinese medicine compound composition for enhancing immunity, the composition is made from the following raw materials by weight:

10 parts of Astragalus, 5 parts of Wolfberry, 5 parts of Ophiopogon japonicus, 1 part of Schisandra, 5 parts of Poria, 4 parts of Angelica, 5 parts of sea buckthorn, 5 parts of Hangbaiju.

The traditional Chinese medicine compound composition with the effect of enhancing immunity of the present invention is prepared from the traditional Chinese medicine compound composition and a pharmaceutically acceptable carrier into oral preparations or external preparations, including tablets, dispersible tablets, effervescent tablets, and oral disintegrating tablets. Dissolving tablets, lozenges, chewable tablets, hard capsules, soft capsules, granules, pills, powders, drop pills, oral liquids, sprays, gels, ointments, poultices, patches, liniments , lotion, coating agent.

The traditional Chinese medicine compound composition provided by the invention has a good effect of enhancing immunity, and can be used for preparing the auxiliary function for treating other diseases caused by low immunity, such as sub-health state or susceptible fatigue state or application in health care products.

Under the guidance of traditional Chinese medicine theory, the present invention selects 8 herbs such as astragalus, angelica, Ophiopogon japonicus, schisandra, medlar, chrysanthemum, tuckahoe and sea buckthorn for compatibility, and determines the relationship between functional components and functions. The dosage of medicinal materials enables synergy between the components. The prescription uses astragalus to invigorate the spleen and qi, and angelica to nourish blood and activate blood. Ophiopogon japonicus nourishes yin and promotes body fluid, Schisandra chinensis nourishes yin and astringes sweat, replenishes qi and generates body fluid, and yin fluid is recovered. Lycium barbarum nourishes liver and kidney, chrysanthemum clears heat and calms liver, and nourishes liver and kidney. Poria strengthens the spleen and relieves dampness and calms the heart, while sea buckthorn nourishes the spleen, nourishes qi and eliminates food. All the medicines in the prescription are both medicinal and edible. The medicinal properties are gentle and suitable for long-term use by the elderly. The polysaccharide components of the 8 traditional Chinese medicines in the prescription all have immune-enhancing effects. In addition, astragalus saponins, total flavonoids of chrysanthemum, total flavonoids of astragalus, total flavonoids of sea buckthorn, and lignans of schisandra also have immune-enhancing effects; Flavonoids have anti-tumor effects, and the chlorogenic acid of chrysanthemum has anti-tumor and immunity-enhancing effects.

The traditional Chinese medicine compound composition composed of whole prescriptions can supplement qi and blood, strengthen the spleen and nourish the liver, harmonize yin and yang, can strengthen the body and cultivate the vitality, enhance human immunity, has a mild effect, and has a slow effect, especially suitable for the elderly or immune system. underpowered people. And all the medicines in the present invention are both medicinal and edible, which are safe and effective.

The preparation method of the traditional Chinese medicine compound composition provided by the invention is:

Take Astragalus, Angelica, Ophiopogon, Schisandra, Wolfberry, Chrysanthemum, Poria, Seabuckthorn by weight, add 5-15 times the amount of water or ethanol with a concentration of 10-70% to extract 1 to 3 times, each time 0.5 After 2 hours, the extracts were combined, concentrated under reduced pressure or normal pressure, and vacuum or spray-dried to obtain dry extract powder.

The traditional Chinese medicine compound composition provided by the invention can be prepared into medicines of various dosage forms with convenient and pharmaceutically acceptable carriers, and is convenient for clinical use.

Description of drawings

Figure 1. Phagocytosis index of carbon clearance in mice in each dose group.

Figure 2 shows the percentage of chicken erythrocytes phagocytosed by mouse macrophages in each dose group.

Figure 3 is a graph of the phagocytosis index of chicken erythrocytes phagocytosed by mouse macrophages in each dose group.

Detailed ways

In order to understand the present invention more clearly, the present invention will be further described in detail below with reference to the embodiments. It should be understood that the specific material ratios, process conditions and results described in the examples are only used to illustrate the present invention, and should not and will not limit the present invention described in detail in the claims.

Example 1

1, a kind of Chinese medicine compound composition with the effect of enhancing immunity, it is made of the following raw materials by weight:

40 parts of Astragalus, 20 parts of Lycium barbarum, 20 parts of Ophiopogon japonicus, 10 parts of Schisandra, 20 parts of Poria, 8 parts of Angelica, 14 parts of sea buckthorn, 20 parts of Hangbai chrysanthemum.

2. Preparation process: take 40 parts of Astragalus, 20 parts of Lycium barbarum, 20 parts of Ophiopogon japonicus, 10 parts of Schisandra, 20 parts of Poria, 8 parts of Angelica, 14 parts of sea buckthorn, 20 parts of Hangbaiju, add 12 times the volume of medicinal materials, and the concentration Extract with 70% ethanol twice, 2 hours each time, combine the extracts, concentrate under reduced pressure at 60° C. to obtain a concentrated solution, and vacuum dry at 70° C. to obtain the obtained solution. Among them, the sample components mainly contain 20% of total polysaccharides, 50% of total flavonoids, and 7% of total phenolic acids.

3. Pharmacological experiments:

3.1 Materials and methods

3.1.1 Drugs and Materials

The traditional Chinese medicine compound composition prepared in Example 1.

3.1.2 Animals: 240 SPF grade C57BL/6J mice, weighing 18-22 g.

3.1.3 Feeding conditions: Mice were raised in a barrier system with a temperature of 20-24°C and a humidity of 40%-60%, with free access to food and water.

3.1.4 Dosage design: The immune-enhancing Chinese medicine compound composition prepared in Example 1 was designed into three dose groups of 1.89g/Kg.BW, 3.78g/Kg.BW, and 7.56g/Kg.BW. They are equivalent to 20 times, 10 times, and 5 times the recommended dosage of the human body, respectively. Another normal administration group is designed to be equivalent to 10 times the recommended dosage of the human body, and a model control group is given hydrocortisone at 1.7 mg/dose by gavage. model, a negative control group (distilled water).

3.1.5 Sample preparation: The Chinese medicine compound composition for enhancing immunity prepared in Example 1 was added with a corresponding proportion of taurine powder, and distilled water was added to mix evenly to prepare a suspension with a concentration of 100% and 50%. spare.

3.1.6 Experimental method: SPF grade C57BL/6J mice were divided into 4 experimental groups according to their body weight. Each group used 60 mice and were randomly divided into 6 groups. group, 1 high-dose group, 1 low-dose group), 1 normal dose group without modeling, 1 model group and 1 negative control group, 10 animals in each group. The first group of mouse delayed-type allergy (DTH) assay, the second group of antibody-producing cell detection, the determination of serum hemolysin, the third group of mice carbon clearance test and the fourth group of mouse peritoneal macrophage phagocytosis chicken erythrocyte test . The test samples were given by gavage once a day at the above doses, and the negative control group was given an equal volume of distilled water, with a maximum gavage volume of 4 mL/Kg.BW for 30 consecutive days.

After the preventive administration, the model group was given hydrocortisone by intragastric administration for 3 days continuously, and the body weight, drinking water, diet, etc. were recorded, and continued to be administered by intragastric administration according to each dose group. Hydrocortisone was administered by intragastric administration once a week to maintain The model is stable.

3.2 Test methods and results:

3.2.1 Determination of delayed-type hypersensitivity (DTH) in mice

On the 26th day, 2% (v/v) SRBC was injected intraperitoneally with 0.2 mL per animal, and the animals were sensitized. On the fourth day after sensitization, 20% (v/v) SRBC was subcutaneously injected into the left hind paw of each mouse. 20 μL/only for challenge. The thickness of the same part of the left hind paw of each rat was measured before and 24 hours after the challenge, and the same part was measured three times, and the average value was taken. The difference between the thickness of the foot before and after the challenge was calculated, and the results of each dose group were compared with the model control group by variance analysis. The results are shown in Table 1.

Table 1 The difference of the posterior toe before and after SRBC-induced DTH

group Weight gain (g) Toe posterior degree difference before and after SRBC-induced DTH (mm) negative control group 2.21±0.53 0.36±0.05* model control group 0.67±0.64 0.65±0.32 normal dose group 1.69±0.55 0.25±0.08* low dose group 1.02±0.68 0.42±0.23 medium dose group 1.87±0.46 0.26±0.07* high dose group 1.95±0.59 0.23±0.16*

Note: The ratio of each dose group to the model control group, * means P<0.05.

It can be seen from the above results that the difference between the model control group and the negative control group was significant (P<0.05), indicating that the modeling was successful; compared with the model control group, the middle and high dose groups had significant differences (P<0.05). 0.05), indicating that the middle and high dose groups can enhance the DTH response of mice to SBRC-induced.

3.2.2 Antibody-producing cell detection test

Intraperitoneal injection of 2% (v/v) SRBC 0.2mL/mice, mice were sacrificed by cervical dislocation 4 days after immunization, the spleen was removed, placed in a small plate containing Hank's solution, and the spleen was gently ground to make The cell suspension was filtered through a 200-mesh sieve, centrifuged (1000 r/min) for 10 min, washed twice with Hank's solution, and finally suspended in 5 mL RPMI1640 medium, counted, and adjusted the cell concentration to 5 × 10 ⁶ cells /mL.

Determination of plaque: after heating and dissolving the surface medium (1g agarose plus double distilled water to 100mL), put it into a 45-50 ℃ water bath for insulation, and mix it with Hank's solution of equal pH7.2-7.4 and 2 times the concentration, Dispense into small test tubes, 0.5 mL each, add 50 μL 10% SRBC (v/v, prepared with SA buffer), 20 μL splenocyte suspension (5×10 ⁶ cells/mL), mix quickly, and pour On the glass slide that has been brushed with agarose thin layer, make parallel slices. After the agar is solidified, the glass slide is horizontally buckled on the slide rack, placed in a carbon dioxide incubator and incubated for 1.5h, and then the complement diluted with SA buffer is used. (1:8) was added to the groove of the slide rack, and after 1.5 h of incubation, the number of hemolytic plaques was counted. The results are shown in Table 2.

Table 2 Hemolytic plaque table

group The number of hemolytic plaques (/10⁵spleen cells) negative control group 97.33±9.63* model control group 77.13±9.21 normal dose group 135.15±9.07* low dose group 121.96±9.30* medium dose group 132.12±9.24* high dose group 153.68±9.15*

Note: The ratio of each dose group to the model control group, * means P<0.05.

It can be seen from the above results that the difference between the model control group and the negative control group was significant (P<0.05), indicating that the modeling was successful; compared with the model control group, each dose group had significant differences (P<0.05). ).

3.2.3 Determination of serum hemolysin

On the 26th day of the test sample, each mouse was intraperitoneally injected with 2% SRBC 0.2 mL/mouse, and blood was collected by enucleating the eyeball on the fourth day after immunization. Separate serum. The serum was diluted with physiological saline, and the serum of different dilutions were placed in the microhemagglutination plate, 100 μL per well, and then 100 μL of 0.5% (v/v) SRBC suspension was added, mixed well, and placed in a wet Cover the flat plate and incubate at 37 °C for 3 h for serum hemolysin determination. The hemagglutination degree was counted, and the corresponding antibody volume was calculated. The results are shown in Table 3.

Table 3 Serum hemolysin table

group Serum hemolysin (antibody number) negative control group 39.32±3.23* model control group 30.54±2.73 normal dose group 45.77±2.55* low dose group 41.46±3.81* medium dose group 45.68±2.73* high dose group 48.76±2.67*

Note: The ratio of each dose group to the model control group, * means P<0.05.

It can be seen from the above results that the difference between the model control group and the negative control group was significant (P<0.05), indicating that the modeling was successful; compared with the model control group, each dose group had significant differences (P<0.05). ). It indicated that each dose group could increase the serum hemolysin level of mice.

3.2.4 Carbon clearance test

Mice in each group were injected with Indian ink (4.0 times diluted with normal saline) 10 mL/kg.BW through the tail vein. 20 μL of blood was collected from each mouse through the venous plexus of the inner canthus at 2 min and 10 min after the ink injection, and quickly added to 2 mL of 0.1% sodium carbonate solution and shaken well. The sodium carbonate solution was used as blank control, and the optical density (OD) was measured at a wavelength of 600 nm with an ultraviolet-visible spectrophotometer. After the mice were sacrificed, the liver and spleen were taken, and the blood stains on the surface of the organs were blotted with filter paper and weighed respectively. The phagocytosis index (a) was calculated according to the formula, and the results are shown in Figure 1.

Comparing each dose group with the negative control group, the difference was significant (P<0.05). The results showed that each dose group of the sample could increase the carbon clearance phagocytosis index in mice.

3.2.5 Assay of phagocytosis of chicken erythrocytes by mouse peritoneal macrophages

Using the semi-in vivo method. Mice in each group were injected intraperitoneally with 20% (v/v) chicken erythrocyte suspension 1 mL/mice, and the mice were sacrificed by neck push-dislocation at 30-minute intervals, and 2 mL/mice of normal saline was injected intraperitoneally. After rotating the mouse plate for 1 min, aspirate 1 mL of the peritoneal wash solution and drop it on two glass slides, put them in an enamel box padded with wet gauze, and incubate at 37°C for 30 min. After incubation, rinsed with normal saline, air-dried, fixed with 1:1 acetone-methanol solution, stained with 4% (v/v) Giemsa-phosphate buffer, rinsed with distilled water and air-dried. Observe 100 macrophages under oil microscope, count the number of macrophages phagocytizing chicken erythrocytes, the total number of chicken erythrocytes phagocytosed by macrophages and the degree of digestion, and calculate the phagocytic rate and phagocytic index according to the formula. The results are shown in Figure 2 shown.

Compared with the model control group, the phagocytic percentage and phagocytic index of chicken erythrocytes phagocytosed by mouse macrophages in each dose group were significantly different (P<0.05).

Example 2

1, a kind of Chinese medicine compound composition with the effect of enhancing immunity, it is made of the following raw materials by weight:

20 parts of Astragalus, 10 parts of Lycium barbarum, 10 parts of Ophiopogon japonicus, 5 parts of Schisandra, 10 parts of Poria, 4 parts of Angelica, 7 parts of sea buckthorn, 10 parts of Hangbaiju.

2. Preparation process: take 20 parts of Astragalus, 10 parts of Lycium barbarum, 10 parts of Ophiopogon japonicus, 5 parts of Schisandra, 10 parts of Poria, 4 parts of Angelica sinensis, 7 parts of sea buckthorn, 10 parts of Hangbai chrysanthemum, add 10 times the amount, and the concentration is 40% The ethanol was extracted three times for 1 hour each time, and the extracts were combined and concentrated under reduced pressure at 80°C to obtain a concentrated solution, which was then vacuum-dried at 80°C. The sample components mainly contain 32% of total polysaccharides, 33% of total flavonoids and 8% of total phenolic acids.

3. Pharmacological experiments:

According to the test method of the pharmacological experiment in the above-mentioned embodiment 1, the DTH thickening hemolytic plaque number, serum hemolysin, carbon clearance phagocytic index a and phagocytosis of chicken erythrocytes of the traditional Chinese medicine compound composition for enhancing the immunity of the body in Example 2 were tested. The phagocytosis rate is shown in Table 4.

Table 4

Note: The ratio of each dose group to the model control group, * means P<0.05.

It can be seen from the above results that the difference between the model control group and the negative control group was significant (P<0.05), indicating that the modeling was successful; compared with the model control group, the middle and high dose groups had significant differences (P<0.05). <0.05), indicating that the middle and high dose groups can enhance the immune function of mice.

Example 3

1, a kind of Chinese medicine compound composition with the effect of enhancing immunity, it is made of the following raw materials by weight:

10 parts of Astragalus, 5 parts of Lycium barbarum, 5 parts of Ophiopogon japonicus, 1 part of Schisandra, 5 parts of Poria, 4 parts of Angelica, 5 parts of sea buckthorn, 5 parts of Hangbaiju.

2. Preparation process: take 10 parts of Astragalus, 5 parts of Lycium barbarum, 5 parts of Ophiopogon japonicus, 1 part of Schisandra chinensis, 5 parts of Poria, 4 parts of Angelica, 5 parts of sea buckthorn, 5 parts of Hangbai chrysanthemum, add 8 times the amount of water to extract 3 times, For 1.5 hours each time, the extracts were combined and concentrated under reduced pressure at 80°C to obtain a concentrated solution, which was then vacuum-dried at 80°C. The sample components mainly contain 40% of total polysaccharides, 25% of total flavonoids and 8% of total phenolic acids.

3. Pharmacological experiments:

According to the test method of the pharmacological experiment in the above-mentioned Example 1, the number of hemolytic plaques, the serum hemolysin, the carbon clearance phagocytic index a and the phagocytic rate of phagocytic chicken erythrocytes of the Chinese medicine compound composition for enhancing the immunity of the body in Example 3 were tested. , as shown in Table 5.

table 5

Note: The ratio of each dose group to the model control group, * means P<0.05.

It can be seen from the above results that the difference between the model control group and the negative control group was significant (P<0.05), indicating that the modeling was successful; compared with the model control group, the middle and high dose groups had significant differences (P<0.05). <0.05), indicating that the middle and high dose groups can enhance the immune function of mice.

The above descriptions are only preferred embodiments of the present invention and are not intended to limit the present invention. Any modification, equivalent replacement or improvement made within the spirit and principle of the present invention shall be included in the protection of the present invention. within the range.

Claims (8)

1. a Chinese medicine compound composition with immunity-enhancing, is characterized in that, it is made by the raw material of following parts by weight: 10-40 servings of Astragalus, 5-20 servings of Lycium barbarum, 5-20 servings of Ophiopogon japonicus, 1-15 servings of Schisandra, 5-20 servings of Poria, 2-15 servings of Angelica, 2-20 servings of sea buckthorn, 5-20 servings of Hangbaiju . 2. the Chinese medicine compound composition of enhancing immunity according to claim 1, is characterized in that, described composition is made by the raw material of following weight portion: 40 parts of Astragalus, 20 parts of Lycium barbarum, 20 parts of Ophiopogon japonicus, 10 parts of Schisandra, 20 parts of Poria, 8 parts of Angelica, 14 parts of sea buckthorn, 20 parts of Hangbai chrysanthemum. 3. the Chinese medicine compound composition of enhancing immunity according to claim 1, is characterized in that, described composition is made by the raw material of following weight portion: 20 parts of Astragalus, 10 parts of Lycium barbarum, 10 parts of Ophiopogon japonicus, 5 parts of Schisandra, 10 parts of Poria, 4 parts of Angelica, 7 parts of sea buckthorn, 10 parts of Hangbaiju. 4. the Chinese medicine compound composition of enhancing immunity according to claim 1, is characterized in that, described composition is made by the raw material of following weight portion: 10 parts of Astragalus, 5 parts of Lycium barbarum, 5 parts of Ophiopogon japonicus, 1 part of Schisandra, 5 parts of Poria, 4 parts of Angelica, 5 parts of sea buckthorn, 5 parts of Hangbaiju. 5. the preparation method of the Chinese medicine compound composition of enhancing immunity according to any one of claim 1～4, is characterized in that, it comprises the following steps: (1) Extraction solvent: 5 to 15 times the volume of water to 70% ethanol for reflux or ultrasonic extraction, and extract 1 to 3 times, 0.5 to 2 hours each time. (2) Concentration: Concentration methods include concentration under reduced pressure at 50-80°C and concentration under normal pressure at 100°C. (3) Drying: Drying methods include vacuum and spray drying. 6. the preparation method of the Chinese medicine compound composition of enhancing immunity according to claim 5, is characterized in that, it comprises the following steps: Take Astragalus, Angelica, Ophiopogon, Schisandra, Lycium barbarum, Chrysanthemum, Poria, Seabuckthorn by weight, add 5-15 times the amount of water or ethanol with a concentration of 10-70% to extract 1 to 3 times, each time 0.5 After 2 hours, the extracts are combined, concentrated under reduced pressure or normal pressure, and vacuum or spray-dried to obtain dry extract powder, which is then prepared with a pharmaceutically acceptable carrier to prepare a medicine or a health care product. 7. the preparation method of a kind of Chinese medicine compound composition of enhancing immunity according to claim 6, is characterized in that, described preparation is oral preparation or external preparation comprises tablet, dispersible tablet, effervescent tablet, oral disintegrating tablet Dissolving tablets, lozenges, chewable tablets, hard capsules, soft capsules, granules, pills, powders, drop pills, oral liquid preparations, sprays, gels, ointments, poultices, patches, liniments , lotion, coating agent. 8. The application of the immunity-enhancing traditional Chinese medicine compound composition according to any one of claims 1 to 4 in the preparation of immunity-enhancing medicines or health-care products.

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