Disclosure of Invention
In order to solve the technical problem, the invention provides a fertilized egg hatching system for schizothorax giganteus. The hatching system has high survival rate of the hatched fish eggs and high production efficiency, and can be produced in large scale in Tibet.
The technical scheme adopted by the invention is as follows:
a fertilized egg incubation system for schizothorax prenanti comprises a water accumulation pool and a plurality of incubation groups arranged in the water accumulation pool, wherein each incubation group comprises a collecting tank and production supports arranged at two sides of the collecting tank, a plurality of incubators are arranged on the production supports side by side, a transverse water pipe is arranged above the production supports, water outlet pipes with valves and corresponding to the incubators are arranged on the transverse water pipes, each water outlet pipe is connected with a water inlet hose, the water accumulation pool and the collecting tank are both provided with water outlets, and a screen is arranged in front of the water outlets of the collecting tank; the incubator comprises an incubation barrel and a water inlet pipe, wherein the water outlet end of the water inlet pipe is inserted into and close to the bottom of the incubation barrel from the top of the incubation barrel, the outer side wall of the upper end of the incubation barrel is provided with an overflow groove in a surrounding mode, the top end of the incubation barrel is higher than the side wall of the overflow groove, the overflow gap of the overflow groove is connected with the diversion groove facing the collecting groove, and the water inlet hose extends into the water inlet pipe.
As the fertilized eggs of the schizothorax prenanti are sinking eggs, the incubator is designed to feed water from top to bottom, the water flow collides with the arc-shaped bottom surface of the incubation barrel under the action of gravity, the water column reflects back to form the water flow from bottom to top, the water flow speed is stable, the fertilized eggs can be kept to roll uniformly up and down, and the sedimentation is avoided.
In order to ensure the dissolved oxygen of the water body, the introduced water source usually adopts well water after aeration, so a large amount of bubbles are usually contained in the transverse water pipe, if the water source is directly introduced into the hatching barrel, a large amount of bubbles are introduced into the bottom of the water body, and fish eggs are driven by the bubbles to float to the liquid surface and overflow from the top together with dead eggs along with the water flow. According to the invention, water flow is introduced by extending the hose into the water inlet pipe, bubbles float upwards in the water inlet pipe and then overflow from the top end of the water inlet pipe, and cannot enter the hatching barrel from the bottom end of the water inlet pipe, so that the generation of bubbles in the barrel is avoided.
The inner diameter of the water inlet pipe is 1-1.5cm, and the outer diameter of the water inlet hose is 2-4mm smaller than the inner diameter of the water inlet pipe. The inner diameter of the water inlet pipe is limited to ensure the impact force of water flowing to the bottom of the hatching barrel, and the outer diameter of the water inlet hose is limited to be 2-4mm smaller than the inner diameter of the water inlet pipe, so that a 1-2mm gap space is formed between the water inlet hose and the water inlet pipe, and bubbles can float upwards in the water inlet pipe.
Preferably, the height of the hatching barrel is 60cm, and the water inlet hose extends to a position 1/3 away from the top end of the water inlet pipe. The height of the hatching barrel is 60cm, and the eggs of the schizothorax bigeminy fish just roll up and down at the position 40-50cm away from the bottom of the incubator under the coordination of water flow, so that the utilization of space is facilitated. The water inlet hose extends to a position 1/3 away from the top end of the water inlet pipe so that air bubbles cannot enter from the bottom end of the water inlet pipe.
The invention also provides a fertilized egg hatching method of schizothorax gigantean, which comprises the following steps:
A. placing the fertilized eggs in a hatching frame in a parallel groove for hatching by flowing water before the emergence period of the otoliths; after the otolith appears, the fertilized eggs are moved into an incubator to be incubated.
B. An external water source flows out of the water inlet hose, and keeps the incubator continuously feeding water through the water inlet pipe, so that the fertilized eggs roll up and down under the action of bottom water flow;
C. the dead eggs float on the water surface and are discharged from an overflow port at the upper part of the incubator to a collecting tank along the water flow, and the dead eggs are fished out;
D. after the fry is taken out of the membrane, the fry in the incubator and the collecting tank are transferred to a parallel tank for culture.
Preferably, the external water source is aerated well water, the temperature of hatching water is 7-10 ℃, the pH value is 7.5-8.5, and the dissolved oxygen is more than or equal to 8 mg/L.
The lower the water temperature is, the longer the incubation period of the fertilized eggs is, and the lower the incubation rate is; the hatching rate of fertilized eggs is obviously reduced and the malformation rate of the newly hatched fries is obviously increased when the water temperature is too high. When the temperature of the fertilized eggs of the schizothorax bigeysei is lower than 7 ℃, the incubation period is obviously increased, and the incubation rate of the fertilized eggs is obviously reduced; the water temperature is higher than 10 ℃, the hatching rate of fertilized eggs is obviously reduced, and the malformation rate of the newly hatched fries is obviously increased.
Preferably, the water flow rate in the parallel groove when the hatching frame is hatched is 0.01-0.02m/s, the water flow rate at the water outlet of the water inlet pipe when the hatching device is hatched is 0.1-0.157m/s, and the water flow rate is 30-60L/min.
The fertilized eggs of schizothorax prenanti are very sensitive to water flow and vibration before the otolith emergence period, the water flow rate exceeds 0.02m/s, the death rate of the fertilized eggs is obviously increased, the water flow rate is lower than 0.01m/s, the water flow exchange is slow, and the fertilized eggs are easy to die due to oxygen deficiency. Controlling the water flow speed of the water outlet of the water inlet pipe to be lower than 0.1m/s during incubation of the incubator, so that fertilized eggs of schizothorax prenanti cannot roll up and down and are accumulated at the bottom, and local oxygen deficiency and water mold breeding are easy to occur; when the water flow rate is higher than 0.157m/s, the fertilized eggs roll up and down too much, so that the fertilized eggs cannot adapt to the fertilized eggs and are easy to die in large quantities.
Further preferably, the hatching density of the hatcher is 0.4-0.6 ten thousand grains per litre of water. Fertilized eggs roll up and down uniformly at the water flow rate of 0.1-0.157m/s, the whole incubator is filled with 67-83% of the fertilized eggs, the space of the incubator is fully utilized, and meanwhile, the fertilized eggs cannot overflow from the top of the incubator; after the hatching density of the fertilized eggs is higher than 0.6 ten thousand eggs per liter of water, the fertilized eggs easily overflow from the top of the incubator.
It is further preferred that one of said drainage openings is provided at each end of said collection trough, and that two drainage openings are provided to facilitate the timely drainage of water from the parallel troughs at a flow rate of 0.1-0.157 m/s.
Preferably, the hatching in said hatching frame is between 40% and 42% of the total hatching time.
Preferably, the mesh diameter of the hatching frame is 1.5mm, so that roes are prevented from flowing away with water flow; the aperture of the screen mesh in the collecting tank is 0.8mm, so that the fry which is earlier in emergence of the film is prevented from escaping.
The invention has the beneficial effects that:
1. the fish egg incubator has the advantages that the occupied area is small, and the number of fertilized eggs capable of being incubated in unit area is greatly increased; after the fertilized eggs die, the fish eggs turn white, the density becomes small, and the fish eggs can flow out from the upper end of the incubator along with water flow, so that the dead eggs do not need to be removed, and a large amount of manpower and financial resources are saved.
2. Because huge palpus schizothorax oosperm is the sinking egg, intake for from top to bottom with the incubator design, rivers strike the convex bottom surface of hatching bucket under the action of gravity, the water column reflects back, form rivers from bottom to top, water velocity is stable, can make the oosperm can keep even rolling from top to bottom, adopt the hose to stretch into the mode of inlet tube and introduce rivers, the bubble just upwards floats in the inlet tube, spill over from the top of inlet tube again, and can not enter into the hatching bucket from the bottom of inlet tube, reduce the production of bubble in the bucket in a large number, avoid healthy roe and spill over along with the bubble together.
3. According to the characteristic that the giant-beard schizothorax fry has strong mobility, the collecting tank is arranged between the two rows of hatchers, so that part of the fry with early membrane emergence can conveniently flow into the collecting tank from the overflow port along with water flow.
4. The incubator disclosed by the invention is combined with the characteristic of fertilized egg sensitivity period of schizothorax prenanti, the incubator is used for incubation before the otolith emergence period and after the otolith emergence period, the incubator disclosed by the invention is suitable for artificial propagation of large-scale schizothorax prenanti, the incubation space is effectively saved and the manpower input is effectively reduced on the premise of ensuring the hatchability of fertilized eggs.
Detailed Description
In order to more clearly and specifically illustrate the technical solution of the present invention, the present invention is further described by the following embodiments. The following examples are intended to illustrate the practice of the present invention and are not intended to limit the scope of the invention.
The water temperature is an important factor influencing the propagation, growth and development of aquatic organisms, can directly influence the embryo development speed, and within a certain temperature range, the embryo development speed is accelerated along with the increase of the water temperature, so that the incubation time is shortened. The effect of water temperature on the hatching rate and time of schizothorax giganteus embryos is shown in fig. 1 and 2. When the water temperature is 16 ℃ or above, the fertilized eggs of the schizothorax giganteus grow to 24 hours and then die completely. The water temperature is 4-13 ℃, and the fertilized eggs can grow until the fish fry is filmed. With the rising of the water temperature, the hatching time and the membrane-out time of the schizothorax bigeminy are gradually reduced, and the hatching speed is gradually increased. The water temperature is 4 ℃, the hatching time and the membrane-forming time of the eggs of the schizothorax bigeminy are longest and are 2040 h and 840 h respectively; the incubation time and the membrane-out time at the water temperature of 13 ℃ are respectively the shortest, are 288 h and 120 h, and are respectively 1752 h and 720 h shorter than the incubation time and the membrane-out time at the water temperature of 4 ℃.
In the production process of artificial propagation of schizothorax prenanti, a great amount of fertilized eggs easily die due to physical vibration in the early stage, so that the hatching modes are all hatching in a hatching frame in still water or microflow water, and the hatching modes have the problems of large occupied area, high possibility of oxygen deficiency and death, water mold breeding, huge workload when dead eggs are picked and the like. Therefore, the sensitive period of the fertilized eggs of the schizothorax giganteus needs to be found out urgently at present, and the hatching mode is improved to improve the production efficiency.
In 2021, 2 months, the eggs of schizothorax juxus are obtained from the breeding base (sinking eggs and non-sticky eggs) of schizothorax yuzu, gabby river, academy of aquatic sciences in agrestics of urban areas of Tibet, two fish eggs are taken and incubated in an incubation frame for the research of the death condition of the fish eggs in each development period under continuous vibration. Taking 6 fish eggs, evenly dividing the 6 fish eggs into two parts respectively, hatching in a parallel groove and a cylindrical incubator in the period from insemination to otolith emergence, and researching the hatching condition in two hatching modes before the otolith emergence period.
Roe sensitive period experiment: hatching the fertilized eggs in a hatching tray (49cm, width 35cm, height 3.5cm), placing the hatching tray in a temperature-controlled glass jar with the length of 1.80, width of 0.5m, depth of 0.5m, and hatching water dissolved oxygen of more than or equal to 8mg/L and water temperature of (10.0 +/-0.2) DEG C. From insemination to membrane production, randomly selecting 180 fish eggs in each development period, averagely dividing into 6 parts, placing 3 parts inA water bath constant temperature oscillator (SHZ-A) for oscillation, determining the oscillation frequency to be 100 r/min (the blank group has lower number of dead eggs and extremely high death rate of 150 r/min) throughA pre-test, converting the oscillation frequency into the water flow rate to be 0.314m/s, and counting the death number of fertilized eggs underA dissecting mirror (Nikon ZM18) until the next development period; 3 parts of the control group are incubated in an incubation tray with the length of the parallel groove of 21 cm, the width of 7 cm and the height of 4 cm, and the number of dead eggs is counted after the next period of development. The Liuhaiping (2019 early development characteristics of schizothorax prenanti) is referred to during each development period of schizothorax prenanti.
Fish egg hatching mode: the 6 fish eggs are respectively divided into two parts (volume method) on average, and are incubated in a parallel tank and a cylindrical incubator in the period from insemination to otolith occurrence, the incubation water is aerated well water, the water temperature is 10.0 +/-0.5 ℃, and the dissolved oxygen is 6.5-8.8 mg/L. The size of the parallel groove is 2.7 m multiplied by 0.5m multiplied by 0.25 m, the size of the hatching tray is 49cm multiplied by 35cm multiplied by 3.5cm, water is fed into one end, and water is drained from the other end. The water body exchange speed is 6-8 min/time. Micro-flowing water in the hatching tray with the flow rate of (0.015 +/-0.002) m/s; dead eggs were manually detected daily and recorded. The cylindrical incubator has an inner diameter of 16 cm, a height of 46 cm, an inverted circular arc bottom, water inlet from the lower end and water outlet from the uppermost end, exchange is carried out for 3 times every 1 min, and the water flow rate is 0.167 +/-0.014 m/s.
Calculating the formula:
fertilization rate/% =100 × number of live eggs/total eggs at midgerm cell stage in flat row groove hatching manner
Dead egg rate/% =100 × dead egg number/total egg number hatched
Net mortality/% = mortality in shock group-mortality in blank group
Hatchability/% =100 x number of emerged larvae/total number of eggs
Fry aberration rate/= 100 x malformed fry number/total fry number
Results of the experiment
The mortality rate of the fish eggs of schizothorax grahami in different development periods under continuous vibration is shown in table 1.
The blank group has the highest death rate in the auditory capsule emergence period, and has no obvious difference (p is more than 0.05) with the same 2-cell period, heart primordium and incubation period, and is obviously higher than that in the rest development period (p is less than 0.05). The death rate of the joints of the continuous vibration group and the late death rate of the primitive intestines are the highest and are obviously higher than that of the rest vibration groups (p is less than 0.05), the death rates of the prophase of the blastoderm, the heart beating phase, the pectoral fin primordium, the anal plate phase, the blood circulation phase and the membrane emergence phase are all 0, the difference between the death rates of the prophase of the blastoderm, the heart beating phase, the pectoral fin primordium, the anal plate phase, the blood circulation phase and the membrane emergence phase is not obvious (p is more than 0.05) and is obviously lower than that of the rest development phases (p is less than 0.05) in the same 4 cell phase, the multicellular phase, the otolith emergence phase, the tail bud emergence phase, the eye crystal formation phase, the muscle effect phase, the heart primordium, the olfactory capsule phase, the eye pigment formation phase and the incubation phase. The net mortality rate is the highest in the body segment emergence period and the later period of the primitive intestine, is obviously higher than that of other shaking groups (p is less than 0.05), the mortality rates in the prophase of the blastoderm, the heart beating period, the pectoral fin primordium, the anal plate period, the blood circulation period and the membrane emergence period are all 0, the difference is not obvious (p is more than 0.05) in the same 2-cell period, 4-cell period, multicellular period, otolith emergence period, the tail bud emergence period, the eye crystal formation period, the muscle effect period, the heart primordium, the olfactory capsule period, the eye pigment formation period and the incubation period, and is obviously lower than that in other development periods (p is less than 0.05).
TABLE 1 fertilized egg mortality of schizothorax at different developmental stages under continuous shaking
The period of vibration sensitivity of schizothorax bigeye roe is concentrated before the otolith emergence period. The total hatching time of the eggs of the schizothorax bigarensis is 460.67 h, 185.8 h is used after the eggs are inseminated to the otolith emergence period, and the total hatching time is 40.33 percent; the otolith emergence period to the time of pellicular emergence 274.87 h accounted for 59.67% of the total incubation time.
Example 1
As shown in fig. 3-5, a fertilized egg incubation system for schizothorax prenanti comprises a water accumulation tank 1 and a plurality of incubation groups 2 arranged in the water accumulation tank 1, wherein each incubation group 2 comprises a collecting tank 4 and production supports 5 arranged on two sides of a parallel tank, a plurality of incubators 3 are arranged on the production supports 5 side by side, a transverse water pipe 6 is arranged above each production support 5, water outlet pipes 61 with valves corresponding to the incubators 3 are arranged on the transverse water pipes 6, each water outlet pipe 61 is connected with a water inlet hose 8, water outlets 9 are arranged on the water accumulation tank 1 and the collecting tank 4, and a screen 10 is arranged in front of the water outlet 9 of the collecting tank 4; the incubator 3 comprises an incubation barrel 31 and a water inlet pipe 7, wherein the water outlet end of the water inlet pipe 7 is inserted into and close to the bottom of the incubation barrel 3 from the top of the incubation barrel 3, the outer side wall of the upper end of the incubation barrel 3 is provided with a spillway groove 32 in a surrounding manner, the top end of the incubation barrel 31 is higher than the side wall of the spillway groove 32, the spillway of the spillway groove 32 is connected with a diversion trench 33 facing the collecting tank 4, and the water inlet hose 8 extends into the water inlet pipe 7.
The incubator disclosed by the invention is similar to CN202011267666.5 in structure, and experiments show that bubbles in a transverse water pipe can be introduced into the incubator by adopting a water inlet pipe to directly feed water, so that a large number of bubbles are generated at the bottom of the incubator, the bubbles can wrap fertilized eggs of schizothorax gigantean and bring a large number of fertilized eggs to the liquid level, and a large number of fertilized eggs are caused to flow away from an overflow port. According to the invention, the hose is additionally arranged, water flow is introduced into the water inlet pipe through the hose, bubbles directly overflow from the top of the water inlet pipe and cannot enter the hatching barrel, and the amount of bubbles in the hatching barrel is obviously reduced.
Example 2
The fertilized egg hatching method of schizothorax gigantean comprises the following steps:
A. placing the fertilized eggs in a hatching frame in a parallel groove for hatching by flowing water before the emergence period of the otoliths; after the otolith appears, the fertilized eggs are moved into an incubator to be incubated.
B. An external water source flows out of the water inlet hose, and keeps the incubator continuously feeding water through the water inlet pipe, so that the fertilized eggs roll up and down under the action of bottom water flow;
C. the dead eggs float on the water surface and are discharged from an overflow port at the upper part of the incubator to a collecting tank along the water flow, and the dead eggs are fished out;
D. after the fry is taken out of the membrane, the fry in the incubator and the collecting tank are transferred to a parallel tank for culture.
In the early stage of incubator hatching, the dead egg can float on the surface of water to discharge to the collecting tank 4 along with rivers from the gap on incubator 3 upper portion, the hatching later stage, the fry goes out the membrane back, because the swimming force of huge palpus schizothorax fry is stronger, partial fry can discharge to collecting tank 4 from the gap along with rivers, sets up the screen cloth in the collecting tank 4, avoids the fry to escape, rivers downward flow to ponding pond 1, discharge from the outlet of ponding pond again. Therefore, in the hatching process, dead eggs in the collecting tank 4 need to be fished out in time, the influence of water mold on the health of the fries in the collecting tank 4 is avoided, after most of the fries are out of the membrane, the hatcher is taken out again, the fries are completely transferred into the collecting tank 4 to be cultured, and meanwhile, the fries in the collecting tank 4 are collected and transferred into the parallel tanks by adopting a siphon method.
The survival rate, the membrane emergence rate and the deformity rate of the fertilized eggs of schizothorax bigfruit by adopting the incubator and the parallel groove of the invention are shown in table 2. The survival rate of fish eggs in the cylindrical incubator is obviously lower than that of the flat grooves (P is more than 0.05) before the period from the fertilized eggs to the otolith emergence, and the difference between the membrane emergence rate of fish seedlings of the fertilized eggs in the cylindrical incubator and the flat grooves is not obvious (P is more than 0.05) after the otolith emergence period. The fry distortion rate in the cylindrical incubator is obviously higher than that in the parallel grooves (P is more than 0.05).
TABLE 2 cylindrical incubator and incubation tray for schizothorax bigeminy, survival rate of roe, membrane yield and malformation rate of hatchlings
Example 3
This example is based on example 1:
the inner diameter of the water inlet pipe 7 is 1cm, and the outer diameter of the water inlet hose 8 is 2mm smaller than the inner diameter of the water inlet pipe 7.
The height of the hatching barrel 31 is 60cm, and the water inlet hose 8 extends to the position 1/3 away from the top end of the water inlet pipe 7.
Example 4
This example is based on example 1:
the inner diameter of the water inlet pipe 7 is 1.5cm, and the outer diameter of the water inlet hose 8 is 4mm smaller than the inner diameter of the water inlet pipe 7.
The height of the hatching barrel 31 is 60cm, and the water inlet hose 8 extends to the position 1/3 away from the top end of the water inlet pipe 7.
As shown in fig. 7, the drainage openings 9 are provided at both ends of the collection tank 4.
As shown in fig. 6, a radial support plate 34 is disposed at the upper part inside the hatching barrel 31, an opening 341 through which the water inlet pipe 7 can penetrate is disposed at the center of the support plate 34, and a hollow 342 for water to overflow is disposed around the opening 341. The flowing water in the hatching barrel 31 flows out from the top of the hatching barrel 31 through the hollow 342, enters the annular overflow groove 32, flows into the water collecting tank 1 through the diversion groove 33 and is discharged from the water outlet. The eggs are not easy to gush out with the overflow due to the upward gushing resistance, and the annular overflow groove 32 is provided to keep the outside of the hatching barrel 31 clean.
A plurality of blades 71 are uniformly distributed on the outer side wall of the water outlet end of the water inlet pipe 7 in the circumferential direction, and the tail ends of the blades 71 can just abut against the circular arc-shaped bottom surface of the hatching barrel 31.
The distance between the water outlet of the water inlet pipe 7 and the bottom surface of the incubator 31 is 2.5 mm.
Example 5
This example is based on example 1:
the inner diameter of the water inlet pipe 7 is 1.3cm, and the outer diameter of the water inlet hose 8 is smaller than the inner diameter of the water inlet pipe 7 by 3 mm.
The height of the hatching barrel 31 is 60cm, and the water inlet hose 8 extends to the position 1/3 away from the top end of the water inlet pipe 7.
As shown in fig. 7, the drainage openings 9 are provided at both ends of the collection tank 4.
Example 6
This example is based on example 2:
the external water source is well water after aeration, the temperature of hatching water is 7-10 ℃, the pH value is 7.5-8.5, and the dissolved oxygen is more than or equal to 8 mg/L.
The water flow speed in the parallel groove during the incubation of the incubation frame is 0.01m/s, the water flow speed at the water outlet of the water inlet pipe during the incubation of the incubator is 0.1m/s, and the water flow is 30L/min.
The hatching in the hatching frame accounts for 40% of the total hatching time.
Example 7
This example is based on example 2:
the external water source is well water after aeration, the temperature of hatching water is 7-10 ℃, the pH value is 7.5-8.5, and the dissolved oxygen is more than or equal to 8 mg/L.
The water flow rate in the parallel groove during hatching of the hatching frame is 0.02m/s, the water flow rate at the water outlet of the water inlet pipe during hatching of the incubator is 0.157m/s, and the water flow rate is 60L/min.
Hatching in the hatching frame accounts for 42% of the total hatching time.
Example 8
This example is based on example 2:
the external water source is well water after aeration, the temperature of hatching water is 7-10 ℃, the pH value is 7.5-8.5, and the dissolved oxygen is more than or equal to 8 mg/L.
The water flow rate in the parallel groove during hatching of the hatching frame is 0.015m/s, the water flow rate at the water outlet of the water inlet pipe during hatching of the incubator is 0.13m/s, and the water flow rate is 50L/min.
Hatching in the hatching frame accounts for 41 percent of the total hatching time.
The mesh diameter of the hatching frame is 1.5mm, and the aperture of the screen 10 in the collecting tank 4 is 0.8 mm.
The above-mentioned embodiments only express the specific embodiments of the present invention, and the description thereof is more specific and detailed, but not construed as limiting the scope of the present invention. It should be noted that, for a person skilled in the art, several variations and modifications can be made without departing from the inventive concept, which falls within the scope of the present invention.