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CN113975350A - Preparation method and product of a kind of poultry intestinal drug-resistant insect egg repellent and application thereof - Google Patents

Preparation method and product of a kind of poultry intestinal drug-resistant insect egg repellent and application thereof Download PDF

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CN113975350A
CN113975350A CN202111232934.4A CN202111232934A CN113975350A CN 113975350 A CN113975350 A CN 113975350A CN 202111232934 A CN202111232934 A CN 202111232934A CN 113975350 A CN113975350 A CN 113975350A
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parts
preparation
intestinal
weight
repellent
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CN113975350B (en
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赵国忠
周新运
姚云平
郭婷
丁凯丽
王新宇
呼振豪
张荣飞
孙静
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Tianjin University of Science and Technology
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Abstract

The invention discloses a preparation method of a poultry intestinal drug-resistant worm egg repellent, a product and an application thereof, wherein the poultry intestinal drug-resistant worm egg repellent is obtained by compounding 100-200 parts by weight of capsaicin feeding aid fiber, 50-70 parts by weight of allicin steady-state skeleton, 1-2 parts by weight of coccidian schizont inhibitor, 1-2 parts by weight of lymphoimmunity enhancer, 10-30 parts by weight of intestinal barrier maintainer and 0.1-1 part by weight of intestinal symbiotic bacteria; the capsaicin feeding aid fiber and the allicin steady-state framework ensure the concentration of the core component allicin in the poultry intestinal drug-resistant worm egg repellent, and the capsaicin feeding aid fiber fermented by the garlic straws can be used as a precursor substrate of the allicin and can promote the feeding and growth of the broilers. The addition of the capsaicin feeding-aid fiber can respectively increase the feed intake and weight gain of each broiler by 161g/7 days and 64.46g/7 days, and the construction of the allicin steady-state framework can respectively increase the contents of allicin and ethylicin in the finished product by 3.46 and 2.90 times.

Description

Preparation method of poultry intestinal drug-resistant worm egg repellent, product and application thereof
Technical Field
The invention belongs to the technical field of livestock breeding, and particularly relates to a preparation method of a poultry intestinal drug-resistant worm egg repellent, a product and application thereof.
Background
Coccidiosis is one of the main factors of intestinal diseases of broiler chickens. Coccidiosis attacks and colonizes in intestinal tracts of broiler chickens to cause fatal damages such as diarrhea, intestinal bleeding, anorexia, intestinal injury and the like. The ill chicken has loose feathers, listlessness, remarkably reduced appetite and feed intake, emaciation, anemia and dehydration phenomena, and the fatality rate is more than 50 percent.
At present, chemical, hormone and antibiotic medicines are widely applied to coccidian elimination, but the resistance of the coccidia is increased, and the problems of medicine toxicity and residue are gradually serious. Therefore, safe, environment-friendly and drug-resistant parasite prevention and control medicines are urgently needed by the livestock breeding industry at present.
The natural herbal plant extract is a potential coccidian repellent. The ancient Chinese book of Shennong Ben Cao Jing mentions that Chang shan, guan Zhong, Lei Wan and Fugu are the earliest recorded anthelmintic drugs in the world. Wherein the dichroa febrifuga rich in quinazolinone alkaloid is considered as a core factor for expelling coccidia. The dichroa febrifuga powder and robenidine can obviously reduce the expelling effect of the eimeria and the eimeria variabilis. In addition, the compound extracts of the Chinese pulsatilla root, the aloe, the radix sophorae flavescentis and the like show stronger drug-resistant worm egg expelling performance. Therefore, a natural extract repellent against drug-resistant eggs in poultry intestines is scientific and feasible.
Disclosure of Invention
This section is for the purpose of summarizing some aspects of embodiments of the invention and to briefly introduce some preferred embodiments. In this section, as well as in the abstract and the title of the invention of this application, simplifications or omissions may be made to avoid obscuring the purpose of the section, the abstract and the title, and such simplifications or omissions are not intended to limit the scope of the invention.
The present invention has been made keeping in mind the above and/or other problems occurring in the prior art.
Therefore, the invention aims to overcome the defects in the prior art and provide a preparation method of a poultry intestinal drug-resistant worm egg repellent.
In order to solve the technical problems, the invention provides the following technical scheme: a method for preparing a poultry intestinal drug-resistant worm egg repellent comprises,
preparing capsaicin dietary fiber: crushing fresh garlic straws with the water content of 60-80% until the stems are 3-5 cm long, mixing with soybean meal, wheat bran and corn flour, fermenting at a constant temperature, drying with cold air after fermentation is finished, grinding and sieving with a 20-mesh sieve to obtain the capsaicin dietary fiber;
constructing an allicin steady-state skeleton: mixing allicin, L-ascorbic acid, sucrose and chitosan into a phosphate buffer solution with the pH value of 4-6 and 0.1mol/L to prepare a compound, slowly dripping each part of the compound into 3-5 parts of saturated beta-cyclodextrin, freezing and thawing to separate out particles, and drying by cold air to prepare an allicin steady-state framework;
preparing an insect egg schizont inhibitor: mixing radix Dichroae powder with ethanol solution, pre-soaking, performing microwave treatment, evaporating and concentrating the leaching solution to obtain paste, and collecting to obtain an insect egg schizont inhibitor;
preparing a lymph immunopotentiator: mixing radix astragali powder, ligusticum chuanxiong hort powder, glutinous rice powder and water, boiling for 20-40 min, separating filtrate to obtain a lymph immunopotentiator, wherein the obtained filter residue is a lymph immunopotentiator fermented product;
preparing an intestinal barrier maintenance matrix: uniformly mixing wheat bran, soybean meal, radix dichroae dregs and a lymph immunopotentiator ferment, then adding a traditional Chinese medicine dreg composite fermentation microbial inoculum, drying by cold air after fermentation is completed until the water content is 10-13%, grinding and sieving to prepare an intestinal barrier system matrix;
preparing an intestinal symbiotic microbial inoculum: compounding bacteroides fragilis, clostridium butyricum, lactobacillus and bifidobacterium to prepare an intestinal symbiotic microbial inoculum;
according to the weight parts, 130-170 parts of capsaicin feeding aid fiber, 30-70 parts of allicin steady-state skeleton, 1-2 parts of coccidian schizont inhibitor, 1-2 parts of lymph immunopotentiator, 10-30 parts of intestinal barrier maintainer and 0.1-1 part of intestinal symbiotic microbial inoculum are compounded to obtain the poultry intestinal drug-resistant worm egg repellent.
As a preferable scheme of the preparation method of the poultry intestinal drug-resistant worm egg repellent, the preparation method comprises the following steps: the preparation of the capsaicin feeding-aid fiber comprises,
according to the weight portion, 270 portions of fresh garlic stalks with the water content of 75% are smashed to 3cm, 20 portions of soybean meal, 20 portions of wheat bran and 30 portions of corn flour are mixed, 1 portion of aspergillus niger, 4 portions of lactic acid bacteria and 1 portion of yeast are added into the mixture, the mixture is fermented for 10 days at the frequency of 42 ℃ and uniformly mixed once every day, and after the fermentation is finished, the mixture is dried by cold air at the temperature of 10 ℃ until the water content is 10%, and the mixture is ground and sieved by a 20-mesh sieve.
As a preferable scheme of the preparation method of the poultry intestinal drug-resistant worm egg repellent, the preparation method comprises the following steps: the construction method of the allicin steady-state skeleton comprises the following steps,
according to parts by weight, 7 parts of allicin, 0.5 part of L-ascorbic acid, 1 part of sucrose and 2 parts of chitosan are mixed into 50 parts of phosphate buffer solution with pH of 6 and 0.1mol/L, each part of the compound is slowly dripped into 5 parts of saturated beta-cyclodextrin, freeze thawing and precipitation are carried out at-18 ℃ for 6 hours, and precipitated particles are dried by cold air at 15 ℃.
As a preferable scheme of the preparation method of the poultry intestinal drug-resistant worm egg repellent, the preparation method comprises the following steps: the insect egg schizont inhibitor is prepared by mixing 3 parts by weight of dichroa root powder with 15 parts by weight of 50% ethanol solution, pre-soaking for 30min, and performing microwave treatment at 540W power for 40min for extraction.
As a preferable scheme of the preparation method of the poultry intestinal drug-resistant worm egg repellent, the preparation method comprises the following steps: the lymph immunopotentiator is prepared from 1 part of astragalus powder, 2 parts of ligusticum chuanxiong hort powder, 15 parts of glutinous rice powder and 40 parts of water in parts by weight by boiling for 40min and separating filtrate.
As a preferable scheme of the preparation method of the poultry intestinal drug-resistant worm egg repellent, the preparation method comprises the following steps: the intestinal barrier vitamin matrix is prepared by uniformly mixing 10 parts by weight of wheat bran, 10 parts by weight of soybean meal, 20 parts by weight of radix dichroae dregs, 70 parts by weight of astragalus membranaceus, ligusticum chuanxiong hort and sticky rice dregs, then adding 0.5 part of a composite fermentation microbial inoculum with the same volume ratio of lactobacillus, saccharomycetes and bacillus, keeping the water content at 70%, fermenting for 15 days, drying with cold air at 10 ℃ until the water content is 10%, and grinding and sieving with a 20-mesh sieve.
As a preferable scheme of the preparation method of the poultry intestinal drug-resistant worm egg repellent, the preparation method comprises the following steps: the Chinese medicine dregs are compounded with fermenting bacterial preparation, wherein,
the activity of lactobacillus is greater than 1 × 109The lactobacillus delbrueckii BWCC61722, the lactobacillus brevis BWCC28564 and the pediococcus pentosaceus BWCC660488 bacterial liquid with the same volume ratio are compounded;
the yeast has activity greater than 1 × 109cfu/g of saccharomyces cerevisiae GDMCC2.167 and zygosaccharomyces rouxii BWCC67360 bacterial liquid with equal volume ratio;
the bacillus has activity greater than 1 × 109cfu/g Bacillus subtilis CMCC (B)63501 bacterial liquid.
As a preferable scheme of the preparation method of the poultry intestinal drug-resistant worm egg repellent, the preparation method comprises the following steps: the intestinal symbiotic microbial inoculum comprises the components with the activity of more than 1 multiplied by 10 in parts by weight93 parts of bacteroides fragilis ATCC 25285, 1 part of clostridium butyricum ZKCC-676, 1 part of lactobacillus ATCC 8014 and 3 parts of bifidobacterium CICC 6068 which are cfu/g.
As a preferable scheme of the preparation method of the poultry intestinal drug-resistant worm egg repellent, the preparation method comprises the following steps: the feed additive is prepared by compounding 150 parts of capsaicin feeding-aid fiber, 50 parts of allicin steady-state skeleton, 1 part of coccidian schizont inhibitor, 2 parts of lymph immunopotentiator, 30 parts of intestinal barrier maintainer and 0.1 part of intestinal symbiotic microbial inoculum according to parts by weight.
The invention further aims to overcome the defects in the prior art and provide a product prepared by the preparation method of the poultry intestinal drug-resistant worm egg repellent.
The invention has the beneficial effects that:
(1) the capsaicin feeding aid fiber and the allicin steady-state framework ensure the concentration of the core component allicin in the poultry intestinal drug-resistant worm egg repellent, and the capsaicin feeding aid fiber fermented by the garlic straws can be used as a precursor substrate of the allicin and can promote the feeding and growth of broilers. The addition of the capsaicin feeding-aid fiber can respectively increase the feed intake and weight gain of each broiler by 161g/7 days and 64.46g/7 days, and the construction of the allicin steady-state framework can respectively increase the contents of allicin and ethylicin in the finished product by 3.46 and 2.90 times.
(2) The invention improves the expelling efficiency of the drug-resistant worm eggs from the perspective of external expelling and internal immune enhancement of the worm egg schizont inhibitor and the lymph immunopotentiator respectively. The two-step addition of the composition can reduce the oocyst value of the drug-resistant eggs by an evaluation level (10-1) and improve the coccidiosis resistance index by about 37.
(3) The intestinal barrier maintenance matrix and the intestinal symbiotic microbial inoculum aim to form an intestinal barrier for preventing secondary infection of drug-resistant ova. The addition of the two can obviously change the pathological value of the intestinal tract (6-1) and reduce the oocyst value of the drug-resistant eggs by an evaluation grade (10-1).
Drawings
In order to more clearly illustrate the technical solutions of the embodiments of the present invention, the drawings needed to be used in the description of the embodiments will be briefly introduced below, and it is obvious that the drawings in the following description are only some embodiments of the present invention, and it is obvious for those skilled in the art to obtain other drawings based on these drawings without inventive exercise. Wherein:
FIG. 1 is a graph showing the comparison of the allicin content in examples of the present invention.
FIG. 2 is a comparison graph of the appearance of the cecum under treatment with the repellent against resistant eggs in the poultry intestine according to the present invention.
Fig. 3 is a graph showing the comparison of the appearance of feces treated with the repellent for resistant eggs in poultry intestines in accordance with the present invention.
Detailed Description
In order to make the aforementioned objects, features and advantages of the present invention more comprehensible, specific embodiments thereof are described in detail below with reference to examples of the specification.
In the following description, numerous specific details are set forth in order to provide a thorough understanding of the present invention, but the present invention may be practiced in other ways than those specifically described and will be readily apparent to those of ordinary skill in the art without departing from the spirit of the present invention, and therefore the present invention is not limited to the specific embodiments disclosed below.
Furthermore, reference herein to "one embodiment" or "an embodiment" means that a particular feature, structure, or characteristic described in connection with the embodiment is included in at least one implementation of the invention. The appearances of the phrase "in one embodiment" in various places in the specification are not necessarily all referring to the same embodiment, nor are separate or alternative embodiments mutually exclusive of other embodiments.
The raw materials used in the invention are as follows: garlic straw: farmlands in Jinxiang county of Shandong province; bean pulp, wheat bran, corn flour and glutinous rice: dawn feed ltd; lactobacillus delbrueckii BWCC61722, Lactobacillus brevis BWCC28564, Pediococcus pentosaceus BWCC660488, Saccharomyces cerevisiae GDMCC2.167, Saccharomyces rouxii BWCC67360, Bacillus subtilis CMCC (B)63501, Bacteroides fragilis ATCC 25285, Clostridium butyricum ZKCC-676, Lactobacillus ATCC 8014, Bifidobacterium CICC 6068: beijing Wanjia first Biotech Co., Ltd; l-ascorbic acid, sucrose, chitosan, β -cyclodextrin: tianjin Xiansi Oppon Kokko Co., Ltd; radix dichroae powder, radix astragali powder and rhizoma ligustici chuanxiong powder: bozhou Mahong pharmaceutical Co., Ltd; feed-grade allicin: ji nan Xin Shuo chemical Co., Ltd; eimeria tenella ETWH, Eimeria necatrix ENYS, Eimeria acervulina EAWY and Eimeria maxima EMHL: the animal health research institute parasite biology laboratory of the Guangdong provincial academy of agricultural sciences; lingnan yellow chicken: institute of animal research, academy of agricultural sciences, Guangdong province; 102, complete broiler feed: feed mills at the animal institute of agriculture academy of sciences, Guangdong province; other raw materials are not specially described and are all generally sold in the market.
Example 1
The embodiment provides a preparation method of a poultry intestinal drug-resistant worm egg repellent, which comprises the following steps:
(1) fermenting the capsaicin feeding-aid fiber: according to the weight portion, 270 portions of fresh garlic straws with the water content of 60-80% are smashed to 3cm, 20 portions of soybean meal, 20 portions of wheat bran and 30 portions of corn flour are mixed, 1 portion of aspergillus niger, 4 portions of lactic acid bacteria and 1 portion of yeast are added into the mixture, the mixture is fermented for 10 days at the frequency of uniformly mixing once every day at 42 ℃, and after the fermentation is finished, the mixture is dried by cold air at 10 ℃ until the water content is 10%, and is ground and sieved by a 20-mesh sieve.
(2) Construction of an allicin steady-state framework: according to the parts by weight, 7 parts of allicin, 0.5 part of L-ascorbic acid, 1 part of sucrose and 2 parts of chitosan are mixed into 50 parts of phosphate buffer solution with pH value of 6 and 0.1 mol/L. Each part of the compound is slowly dripped into 5 parts of saturated beta-cyclodextrin, freeze thawing separation is carried out for 6 hours at the temperature of minus 18 ℃, and separated particles are blown by cold wind at the temperature of 15 ℃.
(3) An insect egg schizont inhibitor: mixing 3 parts of dichroa root powder and 15 parts of 50% ethanol solution in parts by weight, pre-soaking for 30min, and performing microwave treatment at 540W power for 40 min. Evaporating and concentrating the leaching liquor to paste, and using the separated filter residue for subsequent fermentation.
(4) Lymphoid immunopotentiator: 1 part of astragalus powder, 2 parts of ligusticum chuanxiong hort powder, 15 parts of glutinous rice powder and 40 parts of water by weight, boiling for 40min, separating filtrate to obtain the finished product, and using the residual filter residue for subsequent fermentation.
(5) Intestinal barrier maintenance matrix: mixing 10 parts of wheat bran, 10 parts of soybean meal and 20 parts of radix dichroae residue with 70 parts of radix astragali, rhizoma ligustici chuanxiong and glutinous rice residue, and adding 0.5 part of lactobacillus (activity greater than 1 × 10)9The bacterial liquid of the cfu/g lactobacillus delbrueckii BWCC61722, the lactobacillus brevis BWCC28564 and the pediococcus pentosaceus BWCC660488 are compounded in the same volume ratio, the yeast (the activity is more than 1 multiplied by 10)9cfu/g of saccharomyces cerevisiae GDMCC2.167 and zygosaccharomyces rouxii BWCC67360 bacteria liquid in equal volume ratio and bacillus (the activity is more than 1 x 10)9cfu/g bacillus subtilis solution) according to a ratio of 1: 1: 1(v/v/v) composite fermentation inoculum, keeping the water content at 70%, fermenting at normal temperature for 15 days, drying with cold air at 10 ℃ after fermentation is completed until the water content is 10%, grinding and sieving with a 20-mesh sieve.
(6) Intestinal symbiotic bacteria: according to the weight portion, 3 portions of bacteroides fragilis, 1 portion of clostridium butyricum, 1 portion of lactobacillus and 3 portions of bifidobacterium are compounded.
(7) According to the weight parts, 150 parts of capsaicin feeding-aid fiber, 50 parts of allicin steady-state skeleton, 1 part of coccidian schizont inhibitor, 2 parts of lymph immunopotentiator, 30 parts of intestinal barrier maintainer and 0.1 part of intestinal symbiotic bacteria agent are compounded to obtain the poultry intestinal drug-resistant worm egg repellent.
And (3) detecting the allicin content:
weighing 10g of poultry coccidiosis prevention and control feed mate, uniformly mixing with 20mL of methanol, homogenizing, and ultrasonically leaching for 15min at 80W. Transferring the leached sample to a 50mL centrifuge tube, centrifuging at 4 ℃ and 5000r/min for 15min, filtering the supernatant with a 0.45-micron microporous membrane, and measuring the allicin content by high performance liquid chromatography. A chromatographic column: arcus EP C18 (150X 4.6mm,5 μm); column temperature: 25 ℃; allicin mobile phase: acetonitrile/ultrapure water 30/70 (v/v); flow rate: 0.8 mL/min; wavelength: 210 nm; sample introduction amount: 20 mL.
The effect verification experiment of the poultry coccidiosis prevention and control feed mate comprises the following steps:
chicks with significant lean or overweight were culled and 14-day healthy chicks with body weight differences within 5g were randomly grouped into 30 per group. The experimental group is fed with 1g of the poultry intestinal drug-resistant worm egg repellent for two days in each 1kg of feed, and the control group is fed with the corresponding standard feed for two days. After two days, each chick is orally taken with 1.5X 105 coccidian sporulated oocysts (Eimeria tenella, Eimeria necatrix, Eimeria acervulina and Eimeria maxima are compounded in equal proportion), and sufficient feed and sterile water for the experimental group and the control group are continuously provided and are fed for 7 days in a free feeding mode. Lesion values were scored as cecal lesions: 0 minute: normal, no macroscopic lesions; 1 min (+ 1): the blind intestine wall is scattered at a silt point in a small amount, the intestine wall is not thickened, and the content is normal; plus or minus 2 minutes: the number of pathological changes is large, the caecum content is obviously bloody, the caecum wall is slightly thickened, and the content is normal; plus or minus 3 minutes: a large amount of blood or caecum core exists in the caecum, the caecum wall is obviously thickened, and the content of excrement in the caecum is low; plus or minus 4 minutes: cecum enlargement (dead chicken ± 4 points) due to massive blood or intestinal core filling. Oocyst value conversion method: the number of the oocysts discharged by the feces is less than 0.1 multiplied by 105, and the corresponding oocyst value is 0; the number of the oocysts discharged by the feces is 0.1-1.0 multiplied by 105, and the corresponding oocyst value is 1; the number of the oocysts discharged by the feces is 2.0-5.0 multiplied by 105, and the corresponding oocyst value is 10; the number of the oocysts discharged by the feces is 6.0-10.0 multiplied by 105, and the corresponding oocyst value is 20; fecal oocyst discharge > 11.0X 105 corresponds to an oocyst value of 40.
Calculating the coccidiosis resistance index of the broiler chicken:
anticoccidial index (weight gain rate + survival rate) × 100- (lesion value + coccidian egg number)
In addition, broiler intestinal and fecal samples were collected to observe the lesion.
Example 2
Compared with the embodiment 1, the poultry intestinal drug-resistant worm egg repellent is prepared by compounding 130 parts by weight of capsaicin feeding aid fiber, 70 parts by weight of allicin steady-state skeleton, 1 part by weight of coccidian schizont inhibitor, 2 parts by weight of lymph immunopotentiator, 30 parts by weight of intestinal barrier maintainer and 0.1 part by weight of intestinal symbiotic bacteria agent, and other preparation process conditions are the same as those in the embodiment 1.
Example 3
Compared with the embodiment 1, the poultry intestinal drug-resistant worm egg repellent is prepared by compounding 140 parts of capsaicin feeding aid fiber, 60 parts of allicin steady-state skeleton, 1 part of coccidian schizont inhibitor, 2 parts of lymph immunopotentiator, 30 parts of intestinal barrier maintainer and 0.1 part of intestinal symbiotic bacteria agent in parts by weight, and other preparation process conditions are the same as those in the embodiment 1.
Example 4
Compared with the embodiment 1, the poultry intestinal drug-resistant worm egg repellent is prepared by compounding 100 parts by weight of capsaicin feeding aid fiber, 50 parts by weight of allicin steady-state skeleton, 1 part by weight of coccidian schizont inhibitor, 2 parts by weight of lymph immunopotentiator, 30 parts by weight of intestinal barrier maintainer and 0.1 part by weight of intestinal symbiotic bacteria agent, and other preparation process conditions are the same as those in the embodiment 1.
Example 5
Compared with the embodiment 1, the traditional Chinese medicine repellent for the drug-resistant eggs in the poultry intestinal tracts is prepared by compounding 200 parts by weight of capsaicin feeding-assisting fiber, 50 parts by weight of allicin steady-state skeleton, 1 part by weight of coccidian schizont inhibitor, 2 parts by weight of lymph immunopotentiator, 30 parts by weight of intestinal tract barrier maintainer and 0.1 part by weight of intestinal tract symbiotic microbial inoculum, and other preparation process conditions are the same as those in the embodiment 1.
TABLE 1 broiler feed intake and weight gain 7 days after feeding
Figure BDA0003316727950000071
As can be seen from the comparison of examples 1 and 2 in Table 1, the capsaicin dietary fiber fermented from garlic stalks can respectively improve the feed intake and weight gain of each broiler by 161g/7 days and 64.46g/7 days. According to the weight parts, the poultry intestinal tract drug-resistant worm egg traditional Chinese medicine repellent compounded by 150 parts of capsaicin feeding-aid fiber, 50 parts of allicin steady-state skeleton, 1 part of coccidian schizont inhibitor, 2 parts of lymph immunopotentiator, 30 parts of intestinal tract barrier maintainer and 0.1 part of intestinal tract symbiotic bacteria agent has the best intervention effect on the growth of broiler chickens. When the addition amount of the capsaicin feeding aid fiber is less than the range, the feeding and the growth of the broiler chicken are weakened, because the capsaicin feeding aid fiber fermented by the garlic stalks has the functions of expelling insects, promoting diet and strengthening digestion of the allicin; and when the content exceeds the range, the additional capsaicin dietary fiber can dilute the nutrient components of the feed and is not beneficial to the growth of the broilers.
Example 6
The embodiment provides a preparation method of a poultry intestinal drug-resistant worm egg repellent, which comprises the following steps:
(1) fermenting the capsaicin feeding-aid fiber: according to the weight portion, 270 portions of fresh garlic straws with the water content of 60-80% are smashed to 3cm, 20 portions of soybean meal, 20 portions of wheat bran and 30 portions of corn flour are mixed, 1 portion of aspergillus niger, 4 portions of lactic acid bacteria and 1 portion of yeast are inoculated into the mixture for fermentation for 10 days, 10 portions of cold air drying is carried out after the fermentation is finished until the water content is 10%, and the mixture is ground and sieved by a 20-mesh sieve.
(2) An insect egg schizont inhibitor: mixing 3 parts of dichroa root powder and 15 parts of 50% ethanol solution in parts by weight, pre-soaking for 30min, and performing microwave treatment at 540W power for 40 min. Evaporating and concentrating the leaching liquor to paste, and using the separated filter residue for subsequent fermentation.
(3) Lymphoid immunopotentiator: 1 part of astragalus powder, 2 parts of ligusticum chuanxiong hort powder, 15 parts of glutinous rice flour and 40 parts of water by weight, boiling for 40min, separating filtrate to obtain the astragalus powder-ligusticum chuanxiong hort powder, and using the residual filter residue for subsequent fermentation.
(4) Intestinal barrier maintenance matrix: mixing 10 parts of wheat bran, 10 parts of soybean meal and 20 parts of radix dichroae residue with 70 parts of radix astragali, rhizoma ligustici chuanxiong and glutinous rice residue, and adding 0.5 part of lactobacillus (activity greater than 1 × 10)9The bacterial liquid of the cfu/g lactobacillus delbrueckii BWCC61722, the lactobacillus brevis BWCC28564 and the pediococcus pentosaceus BWCC660488 are compounded in the same volume ratio, the yeast (the activity is more than 1 multiplied by 10)9cfu/g of saccharomyces cerevisiae GDMCC2.167 and zygosaccharomyces rouxii BWCC67360 bacteria liquid in equal volume ratio and bacillus (the activity is more than 1 x 10)9cfu/g bacillus subtilis solution) according to a ratio of 1: 1: 1(v/v/v) composite fermentation inoculum, keeping the water content at 70%, fermenting for 15 days, drying with cold air at 10 ℃ after the fermentation is finished until the water content is 10%, grinding and sieving with a 20-mesh sieve.
(5) Intestinal symbiotic bacteria: according to the weight portion, 3 portions of bacteroides fragilis, 1 portion of clostridium butyricum, 1 portion of lactobacillus and 3 portions of bifidobacterium are compounded.
(6) According to the weight parts, 150 parts of capsaicin feeding-aid fiber, 50 parts of allicin, 1 part of coccidian schizont inhibitor, 2 parts of lymph immunopotentiator, 30 parts of intestinal barrier maintainer and 0.1 part of intestinal symbiotic microbial inoculum are compounded to obtain the poultry intestinal drug-resistant worm egg repellent.
Comparative graphs of allicin content in examples 1-6 of the present invention are shown in FIG. 1.
Example 7
In this example, compared with example 1, the leaching and addition of the coccidia schizont inhibitor in (3) were not performed, and other preparation process conditions were the same as example 1.
Example 8
In this example, compared with example 1, the leaching and addition of the lymphoimmunopotentiator in (4) were not performed, and other preparation process conditions were the same as those in example 1.
Example 9
In this example, compared with example 1, the fermentation and addition of the intestinal barrier system substrate in (5) were not performed, and other preparation process conditions were the same as those in example 1.
Example 10
In this example, compared with example 1, the intestinal symbiotic bacterial agent is not added, and other preparation process conditions are the same as those in example 1.
TABLE 2 comparison of anticoccidial indices for the examples
Figure BDA0003316727950000091
As shown in table 2, the survival rate of the broiler chickens treated with the intestinal drug-resistant worm egg expellents of the poultry reaches 100%. From examples 1 to 5, it is known that the main function of the capsaicin dietary fiber is to promote the feeding and growth of poultry and is a precursor of allicin as a core component. In addition, in comparison with examples 1 and 6, allicin is the core of the expelling of drug-resistant eggs, and the construction of the stable carrier of allicin can improve the anti-coccidiosis index by 50 by ensuring the effectiveness of allicin. The merozoite inhibitor and the lymph immunopotentiator respectively improve the expelling efficiency of the drug-resistant eggs from the aspects of external expelling and internal immune enhancement. The addition of the two can reduce the oocyst value of the drug-resistant eggs by an evaluation grade (10-1). The intestinal barrier maintenance system matrix and the intestinal symbiotic microbial inoculum aim to form an intestinal barrier for preventing secondary infection of drug-resistant ova. The addition of the two can obviously reduce the pathological value of the intestinal tract (6-1) and reduce the oocyst value of the drug-resistant eggs by an evaluation grade (10-1).
As can be seen from fig. 2, red blood samples or cheese-like blood clots of the cecum caused by the invasion of eggs, as well as the shedding of intestinal barriers and mucous membranes can be significantly inhibited by the poultry intestinal drug-resistant egg repellents, and the cecum after the invasion can be restored to a healthy state by the poultry intestinal drug-resistant egg repellents.
As can be seen from FIG. 3, the phenomenon of hemafecia of the poultry excrement treated by the poultry intestinal drug-resistant worm egg repellent is obviously reduced, and the excrement is uniform in color and luster. This means that the characteristics of intestinal mucosal congestion and mild enteritis have disappeared and the poultry intestinal drug-resistant worm egg expellant treated broilers have returned to a healthy state.
It should be noted that the above-mentioned embodiments are only for illustrating the technical solutions of the present invention and not for limiting, and although the present invention has been described in detail with reference to the preferred embodiments, it should be understood by those skilled in the art that modifications or equivalent substitutions may be made on the technical solutions of the present invention without departing from the spirit and scope of the technical solutions of the present invention, which should be covered by the claims of the present invention.

Claims (10)

1.一种家禽肠道耐药虫卵驱除剂的制备方法，其特征在于：包括，1. a preparation method of a poultry intestinal drug-resistant insect-egg expelling agent, is characterized in that: comprising, 制备辣素助食纤维：将含水量为60～80％的新鲜蒜秸搅碎至茎长3～5cm，混入豆粕、麦麸和玉米粉，保温发酵，发酵完成后，冷风干燥，研磨过20目筛，制得辣素助食纤维；Preparation of capsaicin dietary fiber: crush fresh garlic straw with a water content of 60-80% until the stem length is 3-5cm, mix with soybean meal, wheat bran and corn flour, keep warm and ferment, after the fermentation is completed, dry in cold air, grind for 20 Mesh sieve to obtain capsaicin dietary fiber; 构建蒜素稳态骨架：将蒜素、L-抗坏血酸、蔗糖和壳聚糖混入pH＝4～6、0.1mol/L的磷酸缓冲液中，制得复配物，将每份复配物缓慢滴加到3～5份饱和β-环糊精中，冻融析出颗粒，冷风吹干，制得蒜素稳态骨架；Construction of allicin steady-state skeleton: Mix allicin, L-ascorbic acid, sucrose and chitosan into a phosphate buffer with pH=4～6, 0.1mol/L to prepare a compound. Add dropwise to 3-5 parts of saturated β-cyclodextrin, freeze and thaw to separate out particles, and dry with cold air to prepare allicin steady-state skeleton; 制备虫卵裂殖体抑制剂：将常山根粉与乙醇溶液混合，在预浸泡后，微波处理后，将浸提液蒸发浓缩至膏状收集，制得虫卵裂殖体抑制剂；Preparation of egg schizont inhibitor: Mix the root powder of Changshan with ethanol solution, after pre-soaking, after microwave treatment, evaporate and concentrate the extract to paste and collect to prepare the schizont inhibitor; 制备淋巴免疫增强剂：将黄芪粉、川穹粉、糯米粉和水混合，煮沸20～40min，分离滤液即得淋巴免疫增强剂，所得滤渣为淋巴免疫增强剂发酵物；Preparation of lymphoid immunity enhancer: Mix astragalus powder, Chuanqiang powder, glutinous rice flour and water, boil for 20-40min, separate the filtrate to obtain the lymphoid immunity enhancer, and the obtained filter residue is the lymphoid immunity enhancer fermentation product; 制备肠道屏障维系基质：将麦麸、豆粕、常山根渣与淋巴免疫增强剂发酵物混匀，随后接入中药渣复合发酵菌剂，发酵完成后冷风干燥至含水量10～13％，研磨过筛，制得肠道屏障维系基质；Preparation of intestinal barrier maintenance matrix: mix wheat bran, soybean meal, root residue of Changshan root with lymphoid immunity enhancer fermentation product, then add Chinese medicine residue compound fermentation inoculum, dry in cold air to moisture content of 10-13% after fermentation, grind Screening to obtain intestinal barrier maintenance matrix; 制备肠道共生菌剂：将脆弱拟杆菌、丁酸梭菌、乳杆菌、双歧杆菌复配，制得肠道共生菌剂；Preparation of intestinal symbiotic bacteria agent: compound Bacteroides fragilis, Clostridium butyricum, Lactobacillus and Bifidobacterium to prepare intestinal symbiotic bacteria agent; 按重量份数计，辣素助食纤维130～170份、蒜素稳态骨架30～70份、球虫裂殖体抑制剂1～2份、淋巴免疫增强剂1～2份、肠道屏障维系剂10～30份、肠道共生菌剂0.1～1份复配，即得所述家禽肠道耐药虫卵驱除剂。In parts by weight, 130-170 parts of capsaicin dietary fiber, 30-70 parts of allicin steady-state skeleton, 1-2 parts of coccidia schizont inhibitor, 1-2 parts of lymphoid immunity enhancer, intestinal barrier 10-30 parts of the maintenance agent and 0.1-1 part of the intestinal symbiotic bacterial agent are compounded to obtain the poultry intestinal drug-resistant insect egg repellent. 2.如权利要求1所述家禽肠道耐药虫卵驱除剂的制备方法，其特征在于：所述制备辣素助食纤维，包括，2. the preparation method of poultry intestinal drug-resistant insect egg repellent as claimed in claim 1, is characterized in that: described preparation capsaicin food aid fiber, comprises, 按重量份数计，将含水量在75％的新鲜蒜秸270份搅碎至3cm混入豆粕20份、麦麸20份和玉米粉30份，向混合物中接入1份黑曲酶、4份乳酸菌和1份酵母菌，以42℃、每天混匀一次的频率发酵10天，发酵完成后10℃冷风干燥至含水量10％，研磨过20目筛。In parts by weight, 270 parts of fresh garlic straw with a moisture content of 75% was crushed to 3 cm, mixed with 20 parts of soybean meal, 20 parts of wheat bran and 30 parts of corn flour, and 1 part of koji niger, 4 parts of Lactic acid bacteria and 1 part of yeast were fermented at 42°C for 10 days at a frequency of mixing once a day, and after fermentation was completed, 10°C cold air was dried to a water content of 10%, and ground through a 20-mesh sieve. 3.如权利要求1所述家禽肠道耐药虫卵驱除剂的制备方法，其特征在于：蒜素稳态骨架构建构建方法包括，3. the preparation method of poultry intestinal drug-resistant insect egg repellent as claimed in claim 1, is characterized in that: the allicin steady-state skeleton construction method comprises, 按重量份数计，蒜素7份、L-抗坏血酸0.5份、蔗糖1份和壳聚糖2份混入50份pH＝6、0.1mol/L的磷酸缓冲液中，每份复配物缓慢滴加到5份饱和β-环糊精中，在-18℃进行6h的冻融析出,析出颗粒以15℃冷风吹干。In parts by weight, 7 parts of allicin, 0.5 part of L-ascorbic acid, 1 part of sucrose and 2 parts of chitosan were mixed into 50 parts of pH=6, 0.1mol/L phosphate buffer, and each part of the compound was slowly dropped Add to 5 parts of saturated β-cyclodextrin, freeze-thaw precipitation at -18°C for 6h, and dry the precipitated particles with cold air at 15°C. 4.如权利要求1所述家禽肠道耐药虫卵驱除剂的制备方法，其特征在于：虫卵裂殖体抑制剂，按重量份数计，由常山根粉3份与15份50％的乙醇溶液混合，在预浸泡30min后，以540W功率微波处理40min浸提而得。4. the preparation method of poultry intestinal drug-resistant insect egg repellent as claimed in claim 1, it is characterized in that: insect egg schizont inhibitor, by weight, is composed of 3 parts and 15 parts 50% of Changshan root powder After pre-soaking for 30min, it was obtained by microwave treatment at 540W for 40min. 5.如权利要求1所述家禽肠道耐药虫卵驱除剂的制备方法，其特征在于：淋巴免疫增强剂，按重量份数计，由黄芪粉1份、川穹粉2份，糯米粉15份，水40份，煮沸40min，分离滤液。5. the preparation method of poultry intestinal drug-resistant insect egg repellent as claimed in claim 1, is characterized in that: lymphoid immunity enhancer, by weight, is composed of 1 part of Astragalus powder, 2 parts of Chuanqiu powder, glutinous rice flour 15 parts, 40 parts of water, boil for 40min, and separate the filtrate. 6.如权利要求1所述家禽肠道耐药虫卵驱除剂的制备方法，其特征在于：肠道屏障维系基质，按重量份数计，麦麸10份、豆粕10份、常山根渣20份与黄芪、川穹和糯米渣70份混匀，随后接入0.5份乳酸菌、酵母菌和芽孢杆菌等体积比复合发酵菌剂，保持含水量在70％，进行15天发酵，发酵完成后10℃冷风干燥至含水量10％,研磨过20目筛制得。6. the preparation method of poultry intestinal drug-resistant insect egg repellent as claimed in claim 1, is characterized in that: intestinal barrier maintains matrix, by weight, 10 parts of wheat bran, 10 parts of soybean meal, 20 parts of Changshan root dregs Mixed with 70 parts of Astragalus, Chuanqiong and glutinous rice residue, then added 0.5 parts of lactic acid bacteria, yeast and Bacillus compound fermentation inoculants in an equal volume ratio, keeping the water content at 70%, and fermented for 15 days. After the fermentation was completed, 10 ℃ Cold air drying to 10% water content, grinding through a 20-mesh sieve. 7.如权利要求1所述家禽肠道耐药虫卵驱除剂的制备方法，其特征在于：中药渣复合发酵菌剂，其中，7. the preparation method of poultry intestinal drug-resistant insect egg repellent as claimed in claim 1, is characterized in that: Chinese medicine dregs compound fermentation bacterial agent, wherein, 乳酸菌由活性大于1×109cfu/g的德氏乳杆菌BWCC61722、短乳杆菌BWCC28564和戊糖片球菌BWCC660488菌液等体积比复配而成；The lactic acid bacteria are compounded by equal volume ratio of Lactobacillus delbrueckii BWCC61722, Lactobacillus brevis BWCC28564 and Pediococcus pentosaceus BWCC660488 with an activity greater than 1×10 9 cfu/g; 酵母菌由活性大于1×109cfu/g的酿酒酵母GDMCC2.167和鲁氏酵母BWCC67360菌液等体积比复配而成；Yeast is compounded by equal volume ratio of Saccharomyces cerevisiae GDMCC2.167 and Russell saccharomyces cerevisiae BWCC67360 with activity greater than 1×10 9 cfu/g; 芽孢杆菌为活性大于1×109cfu/g枯草芽孢杆菌CMCC(B)63501菌液。Bacillus subtilis CMCC(B) 63501 bacterial liquid with activity greater than 1×10 9 cfu/g. 8.如权利要求1所述家禽肠道耐药虫卵驱除剂的制备方法，其特征在于：所述肠道共生菌剂，按重量份数计，由活性大于1×109cfu/g的3份脆弱拟杆菌ATCC 25285、1份丁酸梭菌ZKCC-676、1份乳杆菌ATCC 8014、3份双歧杆菌CICC 6068复配而成。8. The preparation method of the poultry intestinal drug-resistant insect egg repellent according to claim 1, wherein the intestinal symbiotic bacterial agent, in parts by weight, is composed of an active agent greater than 1×10 9 cfu/g 3 copies of Bacteroides fragilis ATCC 25285, 1 copy of Clostridium butyricum ZKCC-676, 1 copy of Lactobacillus ATCC 8014, and 3 copies of Bifidobacterium CICC 6068 are compounded. 9.如权利要求1所述家禽肠道耐药虫卵驱除剂的制备方法，其特征在于：按重量份数计，辣素助食纤维150份、蒜素稳态骨架50份、球虫裂殖体抑制剂1份、淋巴免疫增强剂2份、肠道屏障维系剂30份、肠道共生菌剂0.1份复配而成。9. the preparation method of poultry intestinal drug-resistant insect egg repellent as claimed in claim 1, it is characterized in that: in parts by weight, 150 parts of capsaicin dietary fibers, 50 parts of allicin steady-state skeleton, coccidiostat It is made up of 1 part of colony inhibitor, 2 parts of lymphoid immunity enhancer, 30 parts of intestinal barrier maintenance agent, and 0.1 part of intestinal symbiotic bacteria agent. 10.如权利要求1～9中任一所述家禽肠道耐药虫卵驱除剂的制备方法制得的产品。10. The product obtained by the preparation method of the poultry intestinal drug-resistant insect egg repellent according to any one of claims 1 to 9.
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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN114917330A (en) * 2022-04-12 2022-08-19 天津纳尔生物科技有限公司 A kind of preparation method and application of immune solution for preventing and treating broiler chicken coccidiosis

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1771832A (en) * 2005-08-02 2006-05-17 陆家坤 Garlic health fowl feed
CN105994948A (en) * 2016-06-16 2016-10-12 苏州福德龙水产养殖有限公司 Composite fishery nutritional microcapsule feed and breeding method
CN106551080A (en) * 2016-11-29 2017-04-05 郑州思辩科技有限公司 A kind of Chinese medicine microbiological feed and preparation method thereof
CN106942514A (en) * 2017-04-11 2017-07-14 柳州市强艺种植场 A kind of goose feed
CN109170350A (en) * 2018-11-20 2019-01-11 福泉市桂河农业观光发展有限公司 free-range chicken feed

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1771832A (en) * 2005-08-02 2006-05-17 陆家坤 Garlic health fowl feed
CN105994948A (en) * 2016-06-16 2016-10-12 苏州福德龙水产养殖有限公司 Composite fishery nutritional microcapsule feed and breeding method
CN106551080A (en) * 2016-11-29 2017-04-05 郑州思辩科技有限公司 A kind of Chinese medicine microbiological feed and preparation method thereof
CN106942514A (en) * 2017-04-11 2017-07-14 柳州市强艺种植场 A kind of goose feed
CN109170350A (en) * 2018-11-20 2019-01-11 福泉市桂河农业观光发展有限公司 free-range chicken feed

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
杨英华: "大蒜秸秆添加剂在蛋鸡生产中的应用研究", 《兽药与饲料添加剂》 *
陈帆: "复合添加剂对蛋鸡生产性能、蛋品质及肠道菌群的影响", 《畜禽业》 *

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN114917330A (en) * 2022-04-12 2022-08-19 天津纳尔生物科技有限公司 A kind of preparation method and application of immune solution for preventing and treating broiler chicken coccidiosis

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