CN113946011A - Optical fiber probe for efficiently and accurately detecting NADH fluorescence in skin tissue - Google Patents
Optical fiber probe for efficiently and accurately detecting NADH fluorescence in skin tissue Download PDFInfo
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- CN113946011A CN113946011A CN202111440907.6A CN202111440907A CN113946011A CN 113946011 A CN113946011 A CN 113946011A CN 202111440907 A CN202111440907 A CN 202111440907A CN 113946011 A CN113946011 A CN 113946011A
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- G—PHYSICS
- G02—OPTICS
- G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
- G02B6/00—Light guides; Structural details of arrangements comprising light guides and other optical elements, e.g. couplings
- G02B6/02—Optical fibres with cladding with or without a coating
- G02B6/02042—Multicore optical fibres
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61B—DIAGNOSIS; SURGERY; IDENTIFICATION
- A61B5/00—Measuring for diagnostic purposes; Identification of persons
- A61B5/0059—Measuring for diagnostic purposes; Identification of persons using light, e.g. diagnosis by transillumination, diascopy, fluorescence
- A61B5/0071—Measuring for diagnostic purposes; Identification of persons using light, e.g. diagnosis by transillumination, diascopy, fluorescence by measuring fluorescence emission
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61B—DIAGNOSIS; SURGERY; IDENTIFICATION
- A61B5/00—Measuring for diagnostic purposes; Identification of persons
- A61B5/0059—Measuring for diagnostic purposes; Identification of persons using light, e.g. diagnosis by transillumination, diascopy, fluorescence
- A61B5/0075—Measuring for diagnostic purposes; Identification of persons using light, e.g. diagnosis by transillumination, diascopy, fluorescence by spectroscopy, i.e. measuring spectra, e.g. Raman spectroscopy, infrared absorption spectroscopy
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61B—DIAGNOSIS; SURGERY; IDENTIFICATION
- A61B5/00—Measuring for diagnostic purposes; Identification of persons
- A61B5/44—Detecting, measuring or recording for evaluating the integumentary system, e.g. skin, hair or nails
- A61B5/441—Skin evaluation, e.g. for skin disorder diagnosis
- A61B5/443—Evaluating skin constituents, e.g. elastin, melanin, water
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61B—DIAGNOSIS; SURGERY; IDENTIFICATION
- A61B5/00—Measuring for diagnostic purposes; Identification of persons
- A61B5/48—Other medical applications
- A61B5/4866—Evaluating metabolism
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- G—PHYSICS
- G02—OPTICS
- G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
- G02B6/00—Light guides; Structural details of arrangements comprising light guides and other optical elements, e.g. couplings
- G02B6/44—Mechanical structures for providing tensile strength and external protection for fibres, e.g. optical transmission cables
- G02B6/4401—Optical cables
- G02B6/441—Optical cables built up from sub-bundles
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Abstract
The invention relates to the field of fluorescence detection, in particular to an optical fiber probe for efficiently and accurately detecting NADH fluorescence in skin tissues, which comprises a circular sleeve, a plurality of collecting optical fibers, a plurality of exciting optical fibers and filler, wherein the collecting optical fibers are arranged in the center of the sleeve, the exciting optical fibers are uniformly distributed on one side of the inner wall of the sleeve, the exciting optical fibers are not in contact with the collecting optical fibers, and the filler fills the region except the collecting optical fibers and the exciting optical fibers in the sleeve. When the device is applied, the fiber-optic probe is in contact with the skin. The excitation optical fiber emits ultraviolet light, the ultraviolet light enters the skin, the NADH molecules in the skin are excited to generate fluorescence, and the fluorescence is collected by the collection optical fiber, so that NADH molecule detection is realized. In the invention, the excitation optical fiber surrounds the outer side of the collection optical fiber, the excitation light intensity aiming at the NADH molecule is increased in a mode of enlarging the area, the fluorescence intensity of the NADH molecule is provided, the damage to the skin is reduced, and the method has good application prospect in monitoring the NADH content of skin tissues.
Description
Technical Field
The invention relates to the field of fluorescence detection, in particular to an optical fiber probe for efficiently and accurately detecting NADH fluorescence in skin tissues.
Background
NADH (Nicotinamide adenine dinucleotide) is a chemical substance, is the reduced state of nicotinamide adenine dinucleotide, namely reduced coenzyme I. N is nicotinamide, A is adenine and D is a dinucleotide. NADH plays an important role in maintaining cell growth, differentiation and energy metabolism as well as cytoprotection. And it has been demonstrated that measuring NADH levels in skin tissue monitors metabolic and other related information. Therefore, real-time dynamic detection of NADH levels in skin tissue is of great importance.
The non-invasive measurement of NADH content in skin tissue is the key to the realization of the detection of human mitochondrial function and related diseases. At present, the detection of the NADH content in the non-invasive skin tissue mainly adopts a fluorescence spectrum method, wherein a conventional optical fiber probe adopts a single incident optical fiber and collecting optical fiber structure, and the acquired NADH signal is weak and greatly interfered by diffuse reflection light and fluorescence of other components in the skin tissue. Therefore, the structure of the optical fiber probe is optimized, so that the NADH content in skin tissues is efficiently and accurately measured, and the method has important significance for accurately and non-invasively detecting the functions of human mitochondria and related diseases.
Disclosure of Invention
In order to solve the problems, the invention provides an optical fiber probe for efficiently and accurately detecting NADH fluorescence in skin tissues, which comprises a circular sleeve, a plurality of collecting optical fibers, a plurality of exciting optical fibers and filler, wherein the collecting optical fibers are arranged at the center of the sleeve, the exciting optical fibers are uniformly distributed on one side of the inner wall of the sleeve, the exciting optical fibers are not in contact with the collecting optical fibers, and the filler fills the area except the collecting optical fibers and the exciting optical fibers in the sleeve.
Further, the radius of the excitation fiber is 100 microns.
Further, the collection fiber had a radius of 300 microns.
Further, the excitation fiber and the collection fiber were centered 550 microns apart.
Further, 12 excitation fibers were used.
Furthermore, the end face of the excitation optical fiber is an inclined plane, and the excitation optical fiber is low at one side close to the collection optical fiber; the side of the excitation fiber remote from the collection fiber is high.
Further, near the end face of the collection fiber, the collection fiber has only a core.
Further, at the end face of the collection fiber, the cross-sectional area of the core of the collection fiber is large.
The invention has the beneficial effects that: the invention provides an optical fiber probe for efficiently and accurately detecting NADH fluorescence in skin tissues, which comprises a circular sleeve, a plurality of collecting optical fibers, a plurality of exciting optical fibers and filler, wherein the collecting optical fibers are arranged at the center of the sleeve, the exciting optical fibers are uniformly distributed on one side of the inner wall of the sleeve, the exciting optical fibers are not in contact with the collecting optical fibers, and the filler fills the area except the collecting optical fibers and the exciting optical fibers in the sleeve. When the device is applied, the fiber-optic probe is in contact with the skin. The excitation optical fiber emits ultraviolet light, the ultraviolet light enters the skin, NADH molecules in the skin generate fluorescence, and the collection optical fiber collects the fluorescence, so that NADH molecule detection is realized. In the invention, the excitation optical fiber surrounds the outer side of the collection optical fiber, the excitation light intensity aiming at the NADH molecule is increased in a mode of enlarging the area, the fluorescence intensity of the NADH molecule is provided, the damage to the skin is reduced, and the method has good application prospect in monitoring the NADH content of skin tissues.
The present invention will be described in further detail below with reference to the accompanying drawings.
Drawings
FIG. 1 is a schematic diagram of a fiber optic probe for efficient and accurate detection of NADH fluorescence in skin tissue.
FIG. 2 is a graph of the relationship between the intensity of NADH fluorescence and diffuse reflectance as a function of the radius of the collection fiber for Monte Carlo simulations.
FIG. 3 is a graph of the intensity of Monte Carlo simulated NADH fluorescence and diffuse reflectance versus the center distance between the excitation fiber and the collection fiber.
FIG. 4 is a graph of the intensity of Monte Carlo simulated NADH fluorescence and diffuse reflectance versus the distance between the fiber probe and the skin surface.
In the figure: 1. a sleeve; 2. collecting the optical fibers; 3. an excitation optical fiber; 4. and (4) filling materials.
Detailed Description
In order to make the objects, technical solutions and advantages of the present application more apparent, the present application is further described in detail below by referring to the accompanying drawings and examples.
Example 1
The invention provides an optical fiber probe for efficiently and accurately detecting NADH fluorescence in skin tissues, which comprises a sleeve 1, a collecting optical fiber 2, an exciting optical fiber 3 and a filler 4 as shown in figure 1. The sleeve 1 is circular, and the material of the sleeve 1 is stainless steel material. The collecting optical fiber 2 is arranged in the center of the sleeve 1, the collecting optical fiber 2 is a multimode optical fiber, and the collecting optical fiber 2 comprises a fiber core, a cladding and a coating layer. The collection fiber 2 is a visible glass fiber that can transmit visible light. A plurality of excitation optical fibers 3 are arranged, and the excitation optical fibers 3 are uniformly distributed on the inner side of the inner wall of the sleeve 1. The excitation fiber 3 is also a multimode fiber, and the excitation fiber 3 includes a fiber core, a cladding, and a coating layer. The excitation fiber 3 is an ultraviolet silica fiber. The excitation fiber 3 is not in contact with the collection fiber 2, i.e. there is a distance between the excitation fiber 3 and the collection fiber 2. A filler 4 fills the region of the sleeve 1 other than the collection fiber 2 and the excitation fiber 3. That is, the filler 4 serves to fix the collection fiber 2 and the excitation fiber 3. Preferably, the filling 4 has a certain elasticity.
When the optical fiber is applied, one end of the excitation optical fiber 3 is connected with an ultraviolet light source, and the wavelength of the ultraviolet light source is 340 nanometers. One end of the collection optical fiber 2 is connected with the CCD for detecting the fluorescence intensity. The absorption band of NADH molecules is about 320 nm-380 nm; under the excitation of ultraviolet light with the wavelength of 340 nanometers, NADH emits fluorescence, the wavelength of the fluorescence is about 460 nanometers approximately, and the NADH content in the skin is monitored by detecting the fluorescence intensity of NADH molecules.
In the invention, the excitation fiber 3 surrounds the outer side of the collection fiber 2, the excitation light intensity aiming at the NADH molecule is increased in a large-area expanding mode, the fluorescence intensity of the NADH molecule is improved, the damage to the skin is reduced, and the method has good application prospect in monitoring the NADH content of skin tissues. In addition, the excitation optical fiber 3 surrounds the collection optical fiber 2, so that the size of the probe is reduced, the application or integration is convenient, and the method has important significance for non-invasive detection of mitochondrial functions and related diseases of a human body.
Example 2
On the basis of example 1, the optimum dimensions of the components in the fiber-optic probe were simulated using the monte carlo method. In these simulations, the NADH molecule was set at 90 microns below the skin surface. The number of excitation fibers 3 is 12.
FIG. 2 is a graph of the relationship between the intensity of NADH fluorescence and diffuse reflectance as simulated by Monte Carlo and the radius of the collection fiber 2. The radius of the excitation fiber 3 is fixed at 100 μm. FIG. 2 shows the radius r of the collection fiber 2CWhen the particle size is 300 microns, efficient NADH fluorescence signal collection can be realized.
FIG. 3 is a graph of the intensity of the Monte Carlo simulated NADH fluorescence and diffuse reflectance versus the center distance between the excitation fiber 3 and the collection fiber 2. The radius of the excitation fiber 3 was fixed at 100 microns and the radius of the collection fiber 2 was fixed at 300 microns. Fig. 3 shows that when the center distance d between the excitation fiber 3 and the collection fiber 2 is 550 μm, not only can the NADH fluorescence signal be collected efficiently, but also the interference of diffuse reflection light can be reduced well, and the interference of fluorescence of other components in skin tissue can be reduced effectively. That is, this arrangement allows just the fluorescence of the depth of the skin layer where the NADH molecules are located to be collected, while reducing the interference of the fluorescent substance at other depths. For example, NADH is mainly in the epidermal layer, and the largest interfering substance glycosylation end product (AGE) is in the dermal layer, and by setting the distance, the collection of fluorescence signals of NADH molecules can be increased, and the interference of AGE is reduced, so that the depth resolution is realized.
FIG. 4 is a graph of the intensity of Monte Carlo simulated NADH fluorescence and diffuse reflectance versus the distance p between the fiber probe and the skin surface. FIG. 4 shows that the probe surface should be in close contact with the skin when collecting the fluorescence of NADH molecule.
In the above monte carlo simulation, the end faces of the excitation fiber 3 and the collection fiber 2 are both set to be flat, and the end faces of the excitation fiber 3 and the collection fiber 2 are flush.
Example 3
On the basis of the embodiment 1, the end face of the excitation fiber 3 is an inclined plane, and the excitation fiber 3 is lower at the side close to the collection fiber 2; the excitation fiber 3 is high on the side remote from the collection fiber 2. That is, the end face of each excitation fiber 3 is a slope and is inclined to the side of the collection fiber 2, and the angle of inclination of the end face of each excitation fiber 3 is the same. Thus, the ultraviolet light emitted from the end face of the excitation fiber 3 is inclined toward the side of the collection fiber 2, and the ultraviolet light is more concentrated on the lower side of the collection fiber 2 in the skin tissue, so that the excitation of NADH molecules on the lower side of the collection fiber 2 is enhanced, the NADH molecules can generate stronger fluorescence, and the accuracy of NADH molecule content detection is improved.
Example 4
In example 3, the collection fiber 2 has only a core in the vicinity of the end face of the collection fiber 2. That is, in the vicinity of the end face of the collection fiber 2, the cladding and coating layers outside the core of the collection fiber 2 are removed. The fiber core of the collecting optical fiber 2 is exposed outside, so that the contact area of the fiber core of the collecting optical fiber 2 and the skin is increased, more NADH molecular fluorescence in the skin can conveniently enter the collecting optical fiber 2, and the content of the NADH molecules in the skin can be more accurately measured.
Example 5
In example 4, the cross-sectional area of the core of the collection optical fiber 2 is large at the end face of the collection optical fiber 2. That is, the core thickness of the collection fibers 2 is different: at the end face, the core is thick, inside the sleeve 1, the core is of normal diameter. When the whole optical fiber probe is contacted with the skin, the end face and the side face of the fiber core have more contact areas with the skin, so that more NADH molecules in the skin are subjected to fluorescence coupling to enter the fiber core of the collecting optical fiber 2 and further enter the collecting optical fiber 2, and more accurate detection of the NADH molecule content in the skin is realized.
Further, the filler 4 is below the end faces of the excitation fiber 3 and the collection fiber 2. In use, the fiber-optic probe of the present invention is placed under the skin to be measured, that is, the skin is placed on the fiber-optic probe. The skin's own weight presses on the fiber optic probe. When the filler is lower than the end surfaces of the excitation optical fiber 3 and the collection optical fiber 2, the end surface of the excitation optical fiber 3 and the end surface of the collection optical fiber 2 are in close contact with the skin, the side surface of the fiber core of the collection optical fiber 2 is also in close contact with the skin, the contact area between the skin and the fiber core of the collection optical fiber 2 is increased, NADH molecular fluorescence in the skin can also be coupled into the fiber core from the side surface of the fiber core of the collection optical fiber 2, the intensity of the detected fluorescence is improved, and therefore more accurate monitoring of the NADH molecular content is achieved.
Further, at the end face of the collection fiber 2, the core of the collection fiber 2 is spherical. In the manufacturing process, a laser burning mode can be applied to melt the core of the collection optical fiber 2 into a spherical shape. The spherical core protrudes the filler 4. The spherical core is more easily in contact with the skin. When the skin is in contact with the spherical fiber core, the NADH fluorescence in the skin is more inclined to enter the spherical fiber core at a vertical angle, so that more NADH fluorescence is coupled into the fiber core, the detected fluorescence intensity is improved, and the more accurate detection of the NADH molecular content is realized.
The above description is only exemplary of the present application and should not be taken as limiting the present application, as any modification, equivalent replacement, or improvement made within the spirit and principle of the present application should be included in the scope of protection of the present application.
Claims (8)
1. The optical fiber probe for efficiently and accurately detecting the NADH fluorescence in the skin tissue is characterized by comprising a sleeve, a collection optical fiber, a plurality of excitation optical fibers and fillers, wherein the sleeve is circular, the collection optical fibers are arranged at the center of the sleeve, the excitation optical fibers are uniformly distributed on one side of the inner wall of the sleeve, the excitation optical fibers are not in contact with the collection optical fibers, and the fillers fill the area except the collection optical fibers and the excitation optical fibers in the sleeve.
2. The fiber optic probe for efficiently and accurately detecting NADH fluorescence in skin tissue according to claim 1, wherein: the radius of the excitation fiber was 100 microns.
3. The fiber optic probe for efficiently and accurately detecting NADH fluorescence in skin tissue according to claim 2, wherein: the collection fiber had a radius of 300 microns.
4. The fiber optic probe for efficiently and accurately detecting NADH fluorescence in skin tissue according to claim 3, wherein: the center distance between the excitation fiber and the collection fiber was 550 microns.
5. The fiber optic probe for efficiently and accurately detecting NADH fluorescence in skin tissue according to claim 4, wherein: the number of the excitation fibers is 12.
6. The fiber optic probe for efficiently and accurately detecting NADH fluorescence in skin tissue according to any one of claims 1 to 5, wherein: the end face of the excitation optical fiber is an inclined plane, and the excitation optical fiber is lower at one side close to the collection optical fiber; the side of the excitation fiber far away from the collection fiber is high.
7. The fiber optic probe for efficiently and accurately detecting NADH fluorescence in skin tissue according to claim 6, wherein: the collection fiber has only a core near the end face of the collection fiber.
8. The fiber optic probe for efficiently and accurately detecting NADH fluorescence in skin tissue according to claim 7, wherein: at the end face of the collection fiber, the cross-sectional area of the collection fiber core is large.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202111440907.6A CN113946011A (en) | 2021-11-30 | 2021-11-30 | Optical fiber probe for efficiently and accurately detecting NADH fluorescence in skin tissue |
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| Application Number | Priority Date | Filing Date | Title |
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| CN202111440907.6A CN113946011A (en) | 2021-11-30 | 2021-11-30 | Optical fiber probe for efficiently and accurately detecting NADH fluorescence in skin tissue |
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| CN113946011A true CN113946011A (en) | 2022-01-18 |
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Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101716069A (en) * | 2009-11-17 | 2010-06-02 | 中国科学院安徽光学精密机械研究所 | Human body oxidative stress non-invasive fluorescence detection device and method |
| KR20130142731A (en) * | 2012-06-20 | 2013-12-30 | 한국전기연구원 | Device for detecting fluorescence in the skin |
| CN104545812A (en) * | 2014-12-30 | 2015-04-29 | 中国科学院长春光学精密机械与物理研究所 | Detection depth adjustable non-invasive detection device for human body biochemical criteria |
| CN108007910A (en) * | 2017-12-28 | 2018-05-08 | 中国科学院合肥物质科学研究院 | It is a kind of to be used for skin histology fluorescence spectrum and the depth sensitive optical fibre probe of rear orientation light spectrum in site measurement |
| CN216285807U (en) * | 2021-11-30 | 2022-04-12 | 武汉格谱光电科技有限公司 | Optical fiber probe for efficiently and accurately detecting NADH fluorescence in skin tissue |
-
2021
- 2021-11-30 CN CN202111440907.6A patent/CN113946011A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101716069A (en) * | 2009-11-17 | 2010-06-02 | 中国科学院安徽光学精密机械研究所 | Human body oxidative stress non-invasive fluorescence detection device and method |
| KR20130142731A (en) * | 2012-06-20 | 2013-12-30 | 한국전기연구원 | Device for detecting fluorescence in the skin |
| CN104545812A (en) * | 2014-12-30 | 2015-04-29 | 中国科学院长春光学精密机械与物理研究所 | Detection depth adjustable non-invasive detection device for human body biochemical criteria |
| CN108007910A (en) * | 2017-12-28 | 2018-05-08 | 中国科学院合肥物质科学研究院 | It is a kind of to be used for skin histology fluorescence spectrum and the depth sensitive optical fibre probe of rear orientation light spectrum in site measurement |
| CN216285807U (en) * | 2021-11-30 | 2022-04-12 | 武汉格谱光电科技有限公司 | Optical fiber probe for efficiently and accurately detecting NADH fluorescence in skin tissue |
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