CN113945558B - Method for judging myeloproliferation degree - Google Patents
Method for judging myeloproliferation degree Download PDFInfo
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- CN113945558B CN113945558B CN202111148519.0A CN202111148519A CN113945558B CN 113945558 B CN113945558 B CN 113945558B CN 202111148519 A CN202111148519 A CN 202111148519A CN 113945558 B CN113945558 B CN 113945558B
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Abstract
The invention relates to a method for judging the degree of myeloproliferation, which comprises the following steps: when the bone marrow smear reaches a designated position, starting an ash point camera, and automatically performing full-film scanning on the bone marrow smear by an electronic eyepiece; all the pictures of the bone marrow smear collected by the electronic eyepiece are stored for standby; repeatedly collecting a plurality of picture samples of bone marrow smears; dividing the acquired picture sample according to the thickness and uniformity of bone marrow cells, and generating an algorithm for calculating the thickness and uniformity of a bone marrow smear according to the divided pictures; collecting a whole picture of the bone marrow smear again; calculating the thickness and uniformity of the bone marrow smear by utilizing an algorithm, and selecting a region with moderate thickness and uniform distribution of bone marrow cells in the bone marrow smear; the areas of all mature erythrocytes and nucleated cells in the frame selection area are calculated respectively, the area ratio of the mature erythrocytes to the nucleated cells is calculated, and the degree of myeloproliferation is judged by utilizing the area ratio of the erythrocytes to the nucleated cells in the bone marrow.
Description
Technical Field
The invention belongs to the field of artificial intelligent medical diagnosis, and particularly relates to a method for judging the degree of myeloproliferation.
Background
Bone marrow cell morphology test is one of the key diagnostic means in blood pathology, is commonly used for diagnosing various diseases including leukemia, multiple myeloma, lymphoma, anemia, whole blood cytopenia and the like, and bone marrow proliferation degree judgment is an important content in bone marrow examination, and has important value for diagnosing high-proliferation (such as various leukemia) and low-proliferation (such as aplastic anemia) diseases. The current evaluation method for the degree of myeloproliferation adopts five-stage classification method of the institute of hematology of Chinese medical science, and the region with uniform smear thickness is estimated according to the number ratio of nucleated cells to mature erythrocytes, and is classified into five grades of hyperactive hyperplasia, obvious hyperplastic activation, hypoproliferative hyperplasia and severe dysplasia (see table 1). The method has large discrete degree among each level of the reference interval, the critical value is difficult to grasp, and the current marrow smear is mostly manually examined by a microscope, and the collection and counting of the number of the red blood cells and the nucleated cells are manually carried out, so the method is easily influenced by subjective factors.
Table 1 degree of myeloproliferation the five-stage taxonomic reference interval according to cell number.
Disclosure of Invention
The invention aims to overcome the defects in the prior art and provide a method for judging the degree of myeloproliferation, which is simple to operate, is not influenced by subjective factors of people, and can greatly improve the accuracy of myeloproliferation degree detection.
The invention solves the problems by adopting the following technical scheme: there is provided a method for determining the degree of myeloproliferation comprising the steps of:
s1, enabling a bone marrow smear to reach a designated position, starting an ash point camera, and automatically performing full-film scanning on the bone marrow smear by an electronic eyepiece;
S2, all the pictures of the bone marrow smear acquired by the electronic eyepiece in the step S1 are stored for standby;
s3, repeating the steps S1-S2, and collecting a large number of picture samples of bone marrow smears;
s4, dividing the picture sample acquired in the step S3 according to the thickness and uniformity of bone marrow cells, and generating an algorithm for calculating the thickness and uniformity of bone marrow smears according to the divided pictures;
s5, collecting a whole picture of the bone marrow smear again, and calculating the total area of the bone marrow smear;
S6, calculating the thickness and uniformity of the bone marrow smear by utilizing the algorithm in the step S4, and selecting a region with moderate thickness and uniform distribution of bone marrow cells in the bone marrow smear in a frame manner;
s7, calculating the areas of all mature red blood cells and the areas of nucleated cells in the frame selection area in the step S6 respectively, and calculating the area ratio of the mature red blood cells to the nucleated cells;
S8, observing and recording the myeloproliferation degree of the marrow smear collected in the step S5 under the electronic eyepiece;
S9, obtaining the area ratio of mature red blood cells to nucleated cells in the step S7 corresponding to the myeloproliferation degree in the step S8;
S10, repeating the steps S5-S9, and obtaining the area ratio of mature red blood cells to nucleated cells which correspond to different myeloproliferation degrees respectively;
s11, dividing the range of the area ratio of mature red blood cells to nucleated cells according to the grade of the myeloproliferation degree;
S12, collecting a picture of a bone marrow smear, identifying a frame selection area, calculating the area ratio of mature red blood cells to nucleated cells in the frame selection area in the bone marrow smear, and determining the myeloproliferation degree of the bone marrow smear according to the area ratio range of the step S11.
Preferably, in the step 11, when the proliferation level corresponding to the myeloproliferation degree is hyperplastic hyperactive, the ratio of the area of mature red blood cells to the area of nucleated cells is 1-2.2:1.
Preferably, the ratio of the area of mature red blood cells to the area of nucleated cells is 2.2-5.5:1 when the proliferation level corresponding to the myeloproliferation degree in the step 11 is that proliferation is obviously active.
Preferably, in the step 11, when the proliferation level corresponding to the myeloproliferation degree is proliferation activity, the ratio of the area of mature red blood cells to the area of nucleated cells is 5.5-21:1.
Preferably, the ratio of the area of mature red blood cells to the area of nucleated cells is 21-39:1 when the proliferation level corresponding to the myeloproliferation degree in the step 11 is proliferation reduction.
Preferably, in the step 11, when the proliferation level corresponding to the degree of myeloproliferation is a severe decrease in proliferation, the ratio of the area of mature erythrocytes to the area of nucleated cells is 39- ≡1.
Compared with the prior art, the invention has the following advantages and effects: after the bone marrow smear is scanned by the bone marrow smear scanning system, automatically selecting and rounding all areas with uniform thickness on the Zhang Gusui smear, calculating the area ratio of red blood cells to nucleated cells in the areas, and determining the myeloproliferation condition of the bone marrow smear according to the ratio; compared with the traditional method for determining the myeloproliferation degree by utilizing the ratio of the number of the red blood cells to the nucleated cells, the method has more accurate result, is free from the influence of subjective factors, and especially, a special sample is free from the influence of incomplete observation under a lens.
Drawings
Fig. 1 is a flow chart of the method of operation of the present invention.
Detailed Description
The present invention will be described in further detail by way of examples with reference to the accompanying drawings, which are illustrative of the present invention and not limited to the following examples.
Examples
The invention discloses a method for judging the degree of myeloproliferation, which comprises the following steps:
s1, enabling a bone marrow smear to reach a designated position, starting an ash point camera, and automatically performing full-film scanning on the bone marrow smear by an electronic eyepiece;
S2, all the pictures of the bone marrow smear acquired by the electronic eyepiece in the step S1 are stored for standby;
s3, repeating the steps S1-S2, and collecting a large number of picture samples of bone marrow smears;
s4, dividing the picture sample acquired in the step S3 according to the thickness and uniformity of bone marrow cells, and generating an algorithm for calculating the thickness and uniformity of bone marrow smears according to the divided pictures;
s5, collecting a whole picture of the bone marrow smear again, and calculating the total area of the bone marrow smear;
S6, calculating the thickness and uniformity of the bone marrow smear by utilizing the algorithm in the step S4, and selecting a region with moderate thickness and uniform distribution of bone marrow cells in the bone marrow smear in a frame manner;
s7, calculating the areas of all mature red blood cells and the areas of nucleated cells in the frame selection area in the step S6 respectively, and calculating the area ratio of the mature red blood cells to the nucleated cells;
S8, observing and recording the myeloproliferation degree of the marrow smear collected in the step S5 under the electronic eyepiece;
S9, obtaining the area ratio of mature red blood cells to nucleated cells in the step S7 corresponding to the myeloproliferation degree in the step S8;
S10, repeating the steps S5-S9, and obtaining the area ratio of mature red blood cells to nucleated cells which correspond to different myeloproliferation degrees respectively;
s11, dividing the range of the area ratio of mature red blood cells to nucleated cells according to the grade of the myeloproliferation degree;
S12, collecting a picture of a bone marrow smear, identifying a frame selection area, calculating the area ratio of mature red blood cells to nucleated cells in the frame selection area in the bone marrow smear, and determining the myeloproliferation degree of the bone marrow smear according to the area ratio range of the step S11.
As shown in Table 2 below, when the area ratio of mature red blood cells to nucleated cells in the bone marrow smear is 1-2.2:1, the corresponding myeloproliferative grade is hyperplastic hyperactive, and the result is mainly used for clinical diagnosis of leukemia. When the area ratio of mature red blood cells to nucleated cells in the bone marrow smear is 2.2-5.5:1, the corresponding myeloproliferation grade is obviously active in proliferation, and the result is mainly used for diagnosing leukemia and proliferative anemia clinically. When the area ratio of mature red blood cells to nucleated cells in the bone marrow smear is 5.5-21:1, the corresponding myeloproliferation grade is proliferation active, and the result is mainly used for diagnosing clinical normal bone marrow manifestation and various hematopathy. When the area ratio of mature red blood cells to nucleated cells in the bone marrow smear is 21-39:1, the corresponding myeloproliferation grade is reduced proliferation, and the result is mainly used for diagnosing clinical aplastic anemia and various hematopathies. When the area ratio of mature red blood cells to nucleated cells in the bone marrow smear is 39- ++1, the correspondent myeloproliferation grade is the severe reduction of hyperplasia, and the result is mainly used for diagnosing various hematopathies of clinical aplastic anemia and hypoproliferation.
Table 2 degree of myeloproliferation the five-stage taxonomic reference interval was based on cell area ratio.
In the method for judging the myeloproliferation degree of the embodiment, in the judging process, the reference field of view is more than that of the microscopic examination result, the image data of a large number of bone marrow smears are acquired, the algorithm is automatically calculated without subjectivity, and the method has more accuracy and is easier to operate than the original calculation mode according to the number ratio of red blood cells to nucleated cells.
Furthermore, the foregoing description of the invention is provided by way of example only. All equivalent or simple changes according to the features and principles of the present invention are included in the scope of the present invention. Various modifications or additions may be made to the described embodiments by those skilled in the art, or similar alternatives may be made, without departing from the technical solution of the invention or exceeding the scope of the invention as defined in the claims.
Claims (6)
1. A method for determining the degree of myeloproliferation comprising the steps of: s1, enabling a bone marrow smear to reach a designated position, starting an ash point camera, and automatically performing full-film scanning on the bone marrow smear by an electronic eyepiece;
S2, all the pictures of the bone marrow smear acquired by the electronic eyepiece in the step S1 are stored for standby;
s3, repeating the steps S1-S2, and collecting a large number of picture samples of bone marrow smears;
s4, dividing the picture sample acquired in the step S3 according to the thickness and uniformity of bone marrow cells, and generating an algorithm for calculating the thickness and uniformity of bone marrow smears according to the divided pictures;
s5, collecting a whole picture of the bone marrow smear again, and calculating the total area of the bone marrow smear;
S6, calculating the thickness and uniformity of the bone marrow smear by utilizing the algorithm in the step S4, and selecting a region with moderate thickness and uniform distribution of bone marrow cells in the bone marrow smear in a frame manner;
s7, calculating the areas of all mature red blood cells and the areas of nucleated cells in the frame selection area in the step S6 respectively, and calculating the area ratio of the mature red blood cells to the nucleated cells;
S8, observing and recording the myeloproliferation degree of the marrow smear collected in the step S5 under the electronic eyepiece;
S9, obtaining the area ratio of mature red blood cells to nucleated cells in the step S7 corresponding to the myeloproliferation degree in the step S8;
S10, repeating the steps S5-S9, and obtaining the area ratio of mature red blood cells to nucleated cells which correspond to different myeloproliferation degrees respectively;
s11, dividing the range of the area ratio of mature red blood cells to nucleated cells according to the grade of the myeloproliferation degree;
S12, collecting a picture of a bone marrow smear, identifying a frame selection area, calculating the area ratio of mature red blood cells to nucleated cells in the frame selection area in the bone marrow smear, and determining the myeloproliferation degree of the bone marrow smear according to the area ratio range of the step S11; in step S12, after the bone marrow smear scanning system scans the bone marrow smear, automatically selecting all areas with uniform thickness on the whole Zhang Gusui smear, calculating the area ratio of red blood cells to nucleated cells in the areas, and determining the myeloproliferation condition of the bone marrow smear according to the ratio; in the judging process of the myeloproliferation degree, an algorithm built in the marrow smear scanning system is automatically calculated, and subjectivity is avoided; the myeloproliferation degree is classified into five classes, namely hyperactive hyperplasia, remarkably active hyperplasia, reduced hyperplasia and severely reduced hyperplasia.
2. The method according to claim 1, wherein the ratio of the area of mature red blood cells to the area of nucleated cells is 1-2.2:1 when the proliferation level corresponding to the myeloproliferation level in the step S11 is hyperplastic hyperactive.
3. The method according to claim 1, wherein the proliferation level corresponding to the myeloproliferation level in the step S11 is that the ratio of the area of mature red blood cells to the area of nucleated cells is 2.2-5.5:1 when proliferation is significantly active.
4. The method according to claim 1, wherein the ratio of the area of mature red blood cells to the area of nucleated cells is 5.5-21:1 when the proliferation level corresponding to the myeloproliferation level in the step S11 is proliferation active.
5. The method according to claim 1, wherein the ratio of the area of mature red blood cells to the area of nucleated cells is 21-39:1 when the level of proliferation corresponding to the degree of myeloproliferation in step S11 is reduced.
6. The method according to claim 1, wherein the ratio of the area of mature red blood cells to the area of nucleated cells is 39- ≡1 when the proliferation level corresponding to the myeloproliferation level in the step S11 is severely reduced.
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Inventor after: Huang Kai Inventor after: Feng Junlin Inventor after: Li Qiang Inventor after: Lu Tao Inventor after: Lu Ju Inventor before: Huang Zhen Inventor before: Feng Junlin Inventor before: Li Qiang Inventor before: Lu Tao Inventor before: Lu Ju |
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