CN113795757B - Automatic analysis device and automatic analysis method - Google Patents

Abstract

The invention provides an automatic analysis device and an automatic analysis method, which can change the structure of a reagent (bottle) pair according to the actual use condition after determining the structure of the reagent (bottle). An automatic analyzer having a dispensing mechanism for dispensing a plurality of reagents, comprising: a reagent probe for dispensing a reagent filled in a reagent container; a liquid level detection unit that detects a liquid level of the reagent via the reagent probe; a calculation unit that calculates a remaining amount of the reagent in the reagent container based on the liquid level of the reagent detected by the liquid level detection means; and a storage unit that stores data calculated by the calculation unit, wherein the calculation unit calculates an effective test number for each of the reagent containers based on the calculated reagent remaining amounts of the plurality of reagents, registers a reagent pair composed of a combination of the plurality of reagents in the storage unit based on the calculated effective test number, corrects the effective test number for each of the reagent containers in accordance with the use conditions of the plurality of reagents after the start of the analysis, and registers the reagent pair again.

Description

Automatic analysis device and automatic analysis method

Technical Field

The present invention relates to an automatic analyzer and an automatic analysis method for analyzing the component content of a sample such as blood or urine.

Background

Examples of the specimen test for treating a specimen such as blood or urine collected from a patient include biochemical test, immunological test, blood coagulation test, and the like.

For example, in the examination of analyzing components such as blood and urine, it is known to react a sample with a reagent, measure biochemical tests of components such as sugar, lipid, protein, and enzyme, and measure immunological tests of antibodies, hormones, tumor markers, and the like generated when bacteria and viruses enter the body by antigen-antibody reaction.

Biochemical tests are generally performed by using a biochemical automatic analyzer that mixes a sample with a reagent and measures a change in color due to a chemical reaction by using transmitted light, and immunological tests are generally performed by using an immunological test device that adds an antibody bound to a light-emitting body to an antigen contained in a sample to cause an antigen-antibody reaction, washes unbound antibody, and then measures the amount of light emitted by the bound antibody.

In addition, even in biochemical automatic analyzers, there is a method of detecting antibodies contained in a sample using a reagent in which antibodies are immobilized on latex particles. In addition, there are also items of measuring time taken until blood coagulation and items of measuring a molecular marker involved in blood coagulation reaction by using transmitted light in blood coagulation test.

In order to perform efficient analysis in an automatic analysis device, there is a function of sequentially switching to the next reagent container when there is no reagent. Here, the reagent mixed with the sample is often composed of 2 kinds of reagents, and thus the reagents are managed for each pair. However, the paired reagents are not necessarily timed to be identical due to molding errors of the reagent containers, errors of the reagent filling amounts, and the like. In addition, in the case where the device is stopped immediately between the suction of the first reagent and the suction of the second reagent, only one of the reagent pairs may be consumed early.

As a background art in the art, there is a technology such as patent document 1. Patent document 1 discloses a "system in which, when a plurality of reagents are reacted with a sample to be measured, the number of times of use of each reagent pair, which is a combination of a first reagent bottle and a second reagent bottle, is calculated based on the number of times of use of the first reagent and the number of times of use of the second reagent, and when the number of times of use of the remaining reagent pair is small, a reagent pair to be excluded from the reagent pair for reaction with the sample can be selected.

Patent document 2 discloses "an automatic analyzer that obtains a relational expression determined by past data relating to a drive signal amount required for a drive means after a detection means detects a liquid surface and before a reagent probe stops in a reagent and the number of times of dispensing of the reagent", calculates a current predicted reagent remaining amount from the drive signal amount calculated from the relational expression, and determines a reagent remaining amount from a comparison between the current predicted reagent remaining amount and a previous predicted reagent remaining amount, thereby performing stop control of dispensing ".

Prior art literature

Patent literature

Patent document 1: japanese patent laid-open publication 2016-95147

Patent document 2: japanese patent laid-open No. 2007-322241

Disclosure of Invention

Problems to be solved by the invention

However, in the method described in patent document 1, the remaining reagent cannot be used up, and the reagent may be consumed in vain. The number of times of remaining use of each bottle pair in patent document 1 is a theoretical value calculated by a calculation unit, and for example, when the number of times actually measurable is different from the prediction of the number of times of usable times calculated before the start of analysis due to a molding error of a reagent container, an error of a reagent filling amount, or the like, the amount of reagent remaining without exhaustion increases.

Further, in patent document 2, management of each reagent pair is not assumed, and, as in patent document 1, molding errors of the reagent containers and errors of the reagent filling amounts are not considered, so that there is a problem in calculation accuracy of the reagent remaining amount.

Accordingly, an object of the present invention is to provide an automatic analyzer and an automatic analysis method, which can change the structure of a reagent (bottle) pair in accordance with the actual use situation after determining the structure of the reagent (bottle) pair.

Means for solving the problems

In order to solve the above-described problems, the present invention provides an automatic analyzer having a dispensing mechanism for dispensing a plurality of reagents, comprising: a reagent probe for dispensing a reagent filled in a reagent container; a liquid level detection unit that detects a liquid level of the reagent via the reagent probe; a calculation unit that calculates a remaining amount of the reagent in the reagent container based on the liquid level of the reagent detected by the liquid level detection means; and a storage unit that stores data calculated by the calculation unit, wherein the calculation unit calculates an effective test number for each of the reagent containers based on the calculated reagent remaining amounts of the plurality of reagents, registers reagent pairs each composed of a combination of the plurality of reagents in the storage unit based on the calculated effective test number, calculates a cross-sectional area of each of the reagent containers based on a use condition of the plurality of reagents after an analysis is started, corrects the effective test number for each of the reagent containers based on the calculated cross-sectional area, and registers the reagent pairs again.

The present invention is an automatic analysis method for dispensing a plurality of reagents into sample containers, wherein the number of effective tests per reagent container for each reagent in which the plurality of reagents are stored is calculated, a reagent pair composed of a combination of the plurality of reagents is determined based on the calculated number of effective tests, after starting the analysis, the cross-sectional area of each reagent container is calculated based on the use condition of the plurality of reagents, the number of effective tests per reagent container is corrected based on the calculated cross-sectional area, and the reagent pair is registered again.

Effects of the invention

According to the present invention, it is possible to provide an automatic analyzer and an automatic analysis method, in which the configuration of a reagent (bottle) pair can be changed in accordance with the actual use situation after the configuration of the reagent (bottle) pair is determined.

This allows the reagent to be used up to the end without being consumed in vain.

Other problems, configurations and effects than the above will become apparent from the following description of the embodiments.

Drawings

Fig. 1 is a diagram showing a basic configuration of an automatic analyzer according to an embodiment of the present invention.

FIG. 2 is a diagram showing a basic configuration of a reagent liquid level detecting mechanism of an automatic analyzer according to an embodiment of the present invention.

FIG. 3 is a diagram showing a reagent vessel according to an embodiment of the present invention.

FIG. 4 is a flow chart showing an automatic analysis method (reagent pair registration method) in example 1.

FIG. 5 is a diagram showing an example of the reagent pair in example 1.

Fig. 6A is a diagram showing an example of the reagent pair in example 1.

Fig. 6B is a diagram showing an example of the reagent pair in example 1.

FIG. 7 is a flow chart showing an automatic analysis method (reagent pair re-registration method) in example 1.

FIG. 8 is a diagram showing an example of the reagent pair in example 1.

Fig. 9 is a diagram showing a modification of the effective test calculation of the reagent pair in example 1.

FIG. 10 is a flow chart showing an automatic analysis method (reagent pair re-registration method) in example 2.

FIG. 11 is a diagram showing an example of the reagent pair in example 2.

Detailed Description

The mode for carrying out the present invention will be described in detail below with reference to the accompanying drawings. In addition, the same reference numerals are given to the components having the same function in the drawings in principle, and the description thereof may be omitted.

Integral structure of device

First, a basic structure of an automatic analyzer and a flow of analysis based on the basic structure will be described with reference to fig. 1 and 2. When the sample 2 filled in the sample container 1 is set in the sample tray 3, the sample is sucked by the sample dispensing mechanism 4 and discharged to the reaction container 5.

The reaction vessel 5 containing the sample is moved to the first reagent dispensing position by the rotating operation of the reaction disk 6, and the first reagent dispensing mechanism 7a dispenses the first reagent 8a for analysis from the first reagent vessel 9a to the reaction vessel 5.

Next, the first reagent stirring mechanism 10a stirs the mixed solution in the reaction vessel 5. After a certain period of time, the second reagent dispensing mechanism 7b dispenses the second reagent 8b for analysis from the second reagent vessel 9b to the reaction vessel 5. Next, the second reagent stirring mechanism 10b stirs the mixed solution in the reaction vessel 5.

The reaction vessel 5 into which the second reagent 8b is dispensed is the same as the reaction vessel 5 containing the sample 2 and the first reagent 8a described above. The reaction vessel 5 is maintained at a constant temperature, for example, 37℃by circulating the liquid 11 through a constant temperature bath filled in the lower portion of the reaction tray 6, thereby promoting the reaction and stabilizing the progress of the reaction.

This series of actions is controlled by the control circuit 21. The mixed liquid in the reaction vessel 5 is rotated by the reaction disk 6, and the amount of transmitted light is measured by the transmitted light measuring circuit 22 when passing through the absorbance photometer 12. The transmitted light amount data thus obtained is sent to a PC (personal computer) 23, the concentration of the target component in the sample is calculated by an arithmetic unit in the PC23, the data is stored in a data storage unit, and the arithmetic result is displayed on an output unit 24. The reaction vessel 5 after the reaction is washed by the washing mechanism 13 and reused in the subsequent reaction.

Here, the reagent containers 9a, 9b are provided in the first reagent reservoir 14a and the second reagent reservoir 14b, respectively. As shown in fig. 2, the first reagent dispensing mechanism 7a and the second reagent dispensing mechanism 7b are connected to a control unit (control circuit 21) via a liquid level detection circuit 26, and the remaining amount of the reagent is controlled by information from the liquid level detection circuit 26, and the remaining amount of the reagent is displayed on a reagent control screen of the output unit 24.

In fig. 1, an example in which the reagent dispensing mechanism and the reagent (storage) library are separately configured is shown, but this configuration may not be necessarily adopted. For example, a plurality of reagents may be dispensed by 1 reagent dispensing mechanism, or a plurality of reagents may be stored in 1 reagent library.

Example 1

Next, the remaining amount control of the reagent in the present invention will be described with reference to fig. 3 to 6B. Fig. 3 shows the structure of the reagent vessel 9 used in the present invention and the liquid level for detecting the reagent. Fig. 4 is a flowchart showing the automatic analysis method (reagent pair registration method) of the present embodiment.

In the reagent containers 9a and 9b provided in the first reagent bank 14a and the second reagent bank 14b in fig. 1, the reagent is recognized by an instruction from the input unit 25, and the remaining amount is registered. The identification of the reagent and the remaining amount registration are described as separate operations, but may be performed together (simultaneously).

As a method for identifying the reagent, there is a method in which the individual identifier 16 attached to the reagent container 9 shown in fig. 3 is read by the reading units 15a and 15b, for example. As an example of the individual identifier 16, a bar code, RFID, or the like is given, but the present invention is not limited thereto.

In addition, there is also a method in which the reagent container 9 having no individual identifier 16 is manually input from the operation unit.

The first reagent reservoir 14a and the second reagent reservoir 14b perform a rotating operation by an instruction from the input unit 25. Thus, the reagent containers 9a and 9b move, and each time the reagent passes through the front surfaces of the first reading unit 15a and the second reading unit 15b, the reagent information given to the individual identifiers 16 is read.

The reagent information indicates, for example, several or all of a test item name, a bottle code, a reagent type, a size of a reagent container, a reagent expiration date, a lot, a serial number, dose line information, and the like. In addition, even when the individual identifiers 16 are not given to the reagent containers 9a and 9b, the positions in the first reagent bank 14a and the second reagent bank 14b can be designated from the input unit 25, and the reagent can be identified by inputting the reagent information.

Then, the margin registration is performed. The reagent dispensing mechanism 7 is connected to a liquid level detection circuit 26, and when an instruction for registering the remaining amount of reagent is received from the input unit 25, the operation of the reagent probe 17 is controlled by the control circuit 21 (fig. 2). The information of the capacitance when the tip of the reagent probe 17 reaches the reagent liquid surface is processed by the liquid surface detection circuit 26, and the calculation unit and the data storage unit in the PC23 calculate and store the reagent liquid surface height from the amount of lowering of the reagent probe 17.

In addition, the PC23 calculates the number of effective tests for each reagent vessel based on the reagent liquid level and the cross-sectional area information of the reagent vessel 9, stores the number of effective tests in the data storage unit, and outputs the number of effective tests to the output unit 24 (step S401 in fig. 4). The method of detecting the change in capacitance is described here as being used for the calculation of the reagent liquid level, but other methods such as a method of detecting the pressure in the pipe to which the probe is connected and a method of detecting the change by an optical method may be used.

Next, when a plurality of reagent containers are provided in the same item and the same reagent type, a priority for each reagent type is determined (step S402 in fig. 4). The reagent type is a classification of reagents such as a diluent, a first reagent, and a second reagent.

Next, according to the processing of step S403 in fig. 4, reagent pairs are registered in order of priority from high to low. The priority determination method includes, but is not limited to, an early-to-late order of the opening date and time of the reagent (date and time of the first loading in the apparatus), an early-to-late order of the expiration date of the reagent, an early-to-late order of the remaining amount of the reagent, and an ascending order of the position in the reagent library.

Fig. 5 shows an example of a reagent pair composed of 2 kinds of reagents. Here, examples are shown in which 2 reagent containers capable of performing 400 test analyses are provided in the first reagent reservoir 14a, 5 reagents capable of performing 130 test analyses are provided in the second reagent reservoir 14b, and 2 first reagents are provided at positions 1 to 2 in the first reagent reservoir 14a and 5 second reagents are provided at positions 1 to 5 in the second reagent reservoir 14 b. Here, the priority order is determined assuming that the priority order is in order of the positions from small to large.

In fig. 5, the pair of the first reagent at the position 1 in the first reagent bank 14a is the second reagent at the positions 1 to 4 in the second reagent bank 14 b. At this time, the effective test for each reagent pair was calculated as 130 tests in S501 to S503. Since the remaining amount of the first reagent after consumption in S501 to S503 is 10 tests, the effective test of the reagent pair is 10 tests (S504).

In this case, the remaining amount of the second reagent set at the position 4 in the second reagent pool 14b after consumption in S504 is 120 times, and the second reagent is paired with the reagent set at the position 2 in the first reagent pool 14a (S505). Then, the reagent at position 5 in the second reagent reservoir 14b is paired with the reagent at position 2 in the first reagent reservoir 14a as in S506.

Here, when there is an error in the cross-sectional area information of the reagent container registered in advance due to a molding error of the reagent container or the like, the effective number of tests per reagent container registered at the time of reagent registration may deviate from the number of tests that can be actually analyzed. When the cross-sectional area 18 and the height 19 from the bottom of the reagent vessel to the liquid surface are used for the reagent vessel 9 in fig. 3, the number of effective tests per reagent vessel is expressed by the formula (1).

[ Formula 1]

For example, when the cross-sectional area of the reagent container of the first reagent is smaller than the cross-sectional area information registered in advance by 10% and the cross-sectional area of the reagent container of the second reagent matches the cross-sectional area information registered in advance, the effective test number at the time of reagent balance registration deviates from the actual measurable test number. The remaining amount control of the reagent in this case will be described with reference to fig. 6A. Regarding the first reagent, at the time point when the reagent pair is registered, the cross-sectional area registered in advance is 10% more than the actual cross-sectional area, and therefore, the predicted effective test number is apparently overestimated.

Here, when the liquid level is measured each time the measurement is performed and the effective test number is updated, the effective test number is reduced by 10% more than the initial predicted test number, and the apparent test number reduction in the case of actually performing 130 tests is 143 tests (S601 to S602).

On the other hand, the second reagent consumes the reagent in accordance with the assumption of the number of tests at the time of reagent balance registration. In this case, the effective test number of the reagent at the position 1 in the first reagent reservoir 14a is 0 before the reagent at the position 3 in the second reagent reservoir 14b is used up. In this case, the remaining reagent at position 3 in the second reagent bank 14b is newly paired with the reagent at position 2 provided in the first reagent bank 14a (S604), and the reagents at positions 4 and 5 in the first reagent bank 14a are paired with the reagent at position 2 in the first reagent bank 14a (S605 and S606).

This is schematically illustrated in fig. 6B. The pair shown in S611 to S616 is formed at the time of reagent remaining amount registration, but as a result of reflecting the state of the actual analysis, the pair of S614 is not present, and the pair of S617 is registered instead.

However, in the analysis device, a dose line is created by performing measurement (hereinafter, also referred to as calibration) of a standard sample having a known concentration, and the concentration is calculated by comparing the measurement result of the sample having an unknown concentration with the dose line. For this purpose, a dose line is required for each reagent pair. In addition, before measuring a sample of a subject, it is necessary to manage a sample for measurement accuracy in order to confirm whether or not there is a problem in the states of the device and the reagent.

Therefore, in the automatic analyzer of the present invention, it is checked in advance whether a dose line is registered or a measurement of the accuracy control sample is performed for each reagent pair (S404 of fig. 4), and if the measurement result does not exist, measurement is recommended (performed) (S405 of fig. 4).

Furthermore, the fabrication of the dose line does not necessarily need to be performed for each reagent pair. That is, in the example of fig. 5, in the case where the calibration is performed in the pair of S501, the calibration result of S501 can be applied before the pair of S502 to S505 is used.

However, as described above, the effective test number of the remaining amount of the reagent is different from the actual measurable test number, and when the structure of the reagent pair is changed during the continuous analysis, the measurement of the dose line and the accuracy control sample of the reagent pair that is not actually used is performed, resulting in the reagent being consumed in vain.

The flow of this example of registering reagent pairs again will be described with reference to fig. 7 and 8, in which the remaining test numbers of the reagents are appropriately corrected.

First, after the reagent pair is registered by the reagent remaining amount registration (S701 in fig. 7), when an instruction for measurement is received from the input unit 25, the control circuit 21 controls various mechanisms to start the analysis operation as described in the above-described "overall structure of the apparatus" (S702 in fig. 7).

Thereafter, each time the reagent is sucked, the first reagent dispensing mechanism 7a and the second reagent dispensing mechanism 7b store the liquid surface height of the reagent (reference numeral 19 in fig. 3) from the bottom of the reagent container, the actual number of analyses, in the storage portion of the PC23, and display the remaining amount of the reagent on the reagent management screen of the output portion 24.

Thereafter, the deviation ratio (error rate of the number of measurements) between the effective number of tests of the reagent and the actual number of times of suction at the time of reagent registration is calculated, the effective number of tests is corrected, and reagent pairs are registered again in order of higher priority (S703 to S705 in fig. 7). The error rate of the number of measurements is calculated by equation (2).

[ Formula 2]

Here, in S801 of fig. 8, it is assumed that the 143 test is reduced in the data storage unit of the PC23 when the analysis of 130 tests is actually performed. In this case, when the error rate of the number of measurements is calculated by the following equation (2), the error rate of the number of measurements can be calculated to be 10%.

Next, using the error rate calculated by the formula (2), the effective test number stored in the storage unit of the PC23 is corrected by the formula (3) and reflected in the subsequent margin management (S802 to S805 in fig. 8).

[ Formula 3]

Preferably, after the reagent pair is registered again, it is displayed on the output section 24. In particular, when the structure of the reagent pair is different from the structure of the reagent remaining amount registration, the update of the reagent pair is notified (S706), and if necessary, the execution of calibration and the measurement of control are recommended (executed) (S707).

As described above, the automatic analyzer of the present embodiment includes: the reagent dispensing mechanism 7 for dispensing a plurality of reagents includes: a reagent probe 17 for dispensing the reagent filled in the reagent container 9; a liquid level detection unit (liquid level detection circuit 26) that detects the liquid level of the reagent via the reagent probe 17; an arithmetic unit for calculating the remaining amount of the reagent in the reagent container 9 based on the liquid level of the reagent detected by the liquid level detecting means (liquid level detecting circuit 26); and a storage unit for storing the data calculated by the calculation unit, wherein the calculation unit calculates the effective test number of each reagent container 9 based on the calculated reagent residual amounts of the plurality of reagents, registers a reagent pair composed of a combination of the plurality of reagents in the storage unit based on the calculated effective test number, corrects the effective test number of each reagent container 9 according to the use conditions of the plurality of reagents after the analysis is started, and registers the reagent pair again.

The cross-sectional area 18 of each reagent container 9 is calculated according to the use conditions of the plurality of reagents, the number of effective tests of each reagent container 9 is corrected according to the calculated cross-sectional area 18, and the reagent pair is registered again.

Further, the error rate is calculated as the deviation between the effective test number of the reagent at the time of registration and the actual reagent consumption, and the effective test number is corrected based on the error rate.

In the present embodiment, the method of calculating the error rate of the number of measurements and changing the reagent pair when the reagent pair is shifted to the next pair without the second reagent is described, but the method of calculating the error rate of the number of measurements and the timing of correction are not necessarily limited to the above.

In addition, as the error rate of the number of measurements, the number of tests actually measured when the first reagent vessel 9a is used up and the number of decreases in the effective number of tests calculated and stored in the PC23 at the time of reagent remaining amount registration are compared in the formula (2), but may be considered by dividing each time of the number of analyses.

That is, it is desirable to consume 1 test reagent per 1 dispensing, but the 1 test amount is not necessarily reduced due to the error in the cross-sectional area 18 and the height variation caused by the liquid level detection such as the fluctuation of the liquid level. Therefore, the error rate may be calculated by counting the number of increases or decreases of the effective test number and the number of times, and it is considered that the same number of times of errors may occur (fig. 9).

In order to perform reagent pair change earlier, after a predetermined number of tests of reagent is consumed, the error rate of the number of measurements can be calculated from the actual number of measurements and the number of decreases in effective tests stored in the PC 23. If the number of tests at this time is reduced, the timing of the update of the reagent pair can be advanced.

However, in reality, the fluctuation of the liquid level detection height can be calculated with a more accurate error rate by calculating the number of tests which are collected to some extent or more. Therefore, if the number of tests up to the calculation of the effective test correction can be input from the input unit 25, the user can perform the correction of the effective test at an arbitrary timing.

There are also methods for automatically confirming the remaining amount of the reagent at the start of the operation and correcting the number of the effective tests, and methods for correcting the effective tests when the user registers the remaining amount of the reagent at an arbitrary timing.

In addition, when the configuration of the reagent pair is changed by the re-registration of the reagent pair, the change may be notified by the output unit 24 or a notification means, not shown.

As described above, according to the present embodiment, after the configuration of the reagent (bottle) pair is determined, the configuration of the reagent (bottle) pair can be changed in accordance with the actual use situation, and the reagent can be used up to the end without being consumed in vain.

Example 2

Another embodiment of the present invention will be described with reference to fig. 10. The basic flow of the test number correction by analyzing and reflecting the use condition based on the calculation of the effective test number for each reagent container is the same as that of example 1 (fig. 4), and therefore, detailed description thereof will be omitted and only the differences will be described.

In example 1, the effective test number was updated by detecting the reagent liquid level each time the reagent probe 17 was brought into contact with the reagent liquid level at the time of analysis, and the error rate of the effective test number of the reagent was calculated to perform the re-registration of the reagent pair, but in example 2, the error in the cross-sectional area of the reagent container was corrected.

For example, when the number of actual use tests is small relative to the number of effective tests at the time of registration of the reagent pair, it is predicted that the cross-sectional area of the reagent container is different from the registered cross-sectional area. The cross-sectional area can be calculated by the formula (4) using the liquid level of the reagent from the bottom of the reagent container (reference numeral 19 of fig. 3) and the actual number of analysis tests.

[ Equation 4]

In this case, in the stage of analyzing 130 tests of the bottle in S801 in fig. 8, the actual reagent consumption amount is calculated from the dispensing amount and the number of analysis tests at the position 1 in the first reagent reservoir 14a, and the reagent reservoir is divided by the height 19 from the bottom of the reagent reservoir to the liquid surface, whereby the cross-sectional area of the reagent reservoir is calculated and stored as cross-sectional area information in the data storage unit in the PC23 (S1001 in fig. 10). The number of effective tests is corrected using the cross-sectional area information, and reagent pairs are registered again in order of higher priority (S1002 to S1003 in fig. 10).

Example 3

Next, another embodiment related to a method for correcting the number of effective tests will be described with reference to fig. 11. The flow of the whole reagent remaining amount control is substantially the same as in example 1, and therefore, a detailed description thereof is omitted. The difference is that in example 1, the liquid level of the reagent was measured every time the measurement (analysis) was performed, and the number of effective tests was updated, but in this example, the measurement of the liquid level every time the measurement (analysis) was not performed.

That is, after the reagent remaining amount is registered, the number of effective tests is calculated to constitute a reagent pair, but the remaining amount management thereafter does not reflect the liquid level of each analysis, but the number of times of analysis is reduced by software counting. In this case, the actual number of analysis tests does not deviate from the effective number of tests stored in the PC 23.

However, after dispensing the first reagent, only the number of effective tests of the first reagent is reduced when the device is stopped in an emergency due to a power failure or the like until the second reagent is dispensed. For example, after the reagent pair shown in fig. 5 is registered after the reagent remaining amount is registered, the reagent at position 1 in the first reagent reservoir 14a is consumed 30 times, and the reagent remaining amount when the device is stopped by the emergency stop before the start of the dispensing of the second reagent is schematically shown in fig. 11.

In S1101, the reagent at position 1 in the first reagent reservoir 14a was dispensed 30 times, and thus the number of effective tests was 370 times. However, since the second reagent is dispensed, the number of valid tests of the reagent constituting the position 1 in the pair of second reagent reservoirs 14b is kept 130 times without changing. Thereafter, if the analysis is restarted, the reagent at position 1 in the first reagent reservoir 14a is exhausted before the reagent at position 3 in the second reagent reservoir 14b is exhausted, and the first reagent is not exhausted and 20 test amounts remain.

In order to use up the reagent pair effectively, the process of S1104 is not performed, and instead, the process of S1107 is added to reconstruct the reagent pair.

That is, by automatically registering the reagent pair again before the measurement is restarted after the device is temporarily stopped, even when only one of the reagents constituting the pair is consumed, the reagent pair is registered again by reflecting the analysis condition, and the reagent can be efficiently used up.

The present invention is not limited to the above-described embodiments, and includes various modifications. For example, the above-described embodiments are embodiments described in detail to facilitate understanding of the present invention, and are not necessarily limited to the embodiments having all the configurations described. In addition, a part of the structure of one embodiment may be replaced with the structure of another embodiment, and the structure of another embodiment may be added to the structure of one embodiment. In addition, deletion, and substitution of other structures can be performed for a part of the structures of the embodiments.

Description of the reference numerals

1 … Sample Container

2 … Sample

3 … Sample plate

4 … Sample dispensing mechanism

5 … Reaction vessel

6 … Reaction disk

7 … Reagent divides annotates mechanism

7A … first reagent dispensing mechanism

7B … second reagent dispensing mechanism

8A … first reagent

8B … second reagent

9 … Reagent container

9A … first reagent container

9B … second reagent vessel

10A … first reagent stirring mechanism

10B … second reagent stirring mechanism

11 … Constant temperature tank circulating liquid

12 … Absorptiometer

13 … Cleaning mechanism

14A … first reagent library

14B … second reagent library

15A … first reading section

15B … second reading portion

16 … Individual identifiers

17 … Reagent probe

18 … (Of the reagent vessel 9) cross-sectional area

19 … Height of the bottom of the reagent vessel to the liquid level

21 … Control circuit

22 … Transmitted light measuring circuit

23 … PC (personal computer)

24 … Output part

25 … Input section

26 … Liquid level detection circuit.

Claims (10)

1. An automatic analyzer having a dispensing mechanism for dispensing a plurality of reagents, comprising:

a reagent probe for dispensing a reagent filled in a reagent container;

a liquid level detection unit that detects a liquid level of the reagent via the reagent probe;

A calculation unit that calculates a remaining amount of the reagent in the reagent container based on the liquid level of the reagent detected by the liquid level detection means; and

A storage unit for storing the data calculated by the calculation unit,

The calculation unit calculates the effective test number of each of the reagent containers based on the calculated reagent margins of each of the plurality of reagents, registers a reagent pair composed of a combination of the plurality of reagents in the storage unit based on the calculated effective test number, calculates the actual consumption of each reagent based on the respective dispensing amounts of the plurality of reagents and the predetermined test number after a predetermined test number is performed after the analysis is started, calculates the cross-sectional area of each of the reagent containers by dividing each of the reagent containers by the liquid surface height from the bottom of the reagent container, and corrects each of the effective test numbers of the reagent containers based on the calculated cross-sectional area, and registers the reagent pair again.

2. The automatic analyzer according to claim 1, wherein,

The deviation between the effective test number of the reagent at the time of registration and the actual reagent consumption is calculated as an error rate, and the effective test number is corrected based on the error rate.

3. The automatic analyzer according to claim 1, wherein,

After the start of the analysis, the number of effective tests per one of the reagent containers was corrected in accordance with the number of analysis times reduced by the software count, and the reagent pair was registered again.

4. The automatic analyzer according to claim 1, wherein,

The automatic analyzer includes: and an input unit which can input the number of analyses until the number of valid tests is corrected.

5. The automatic analyzer according to claim 1, wherein,

When the structure of the reagent pair is changed by the re-registration of the reagent pair, a notification is made that the change has occurred.

6. An automatic analysis method for dispensing a plurality of reagents into a sample container, characterized in that,

Calculating the effective test number of each reagent container containing each reagent of the plurality of reagents,

Determining a reagent pair comprising a combination of the plurality of reagents based on the calculated number of effective tests,

After the start of the analysis, calculating the actual consumption of each reagent based on the respective dispensing amounts of the plurality of reagents and the predetermined number of experiments after the predetermined number of experiments is performed, dividing each reagent container by the liquid level from the inside of the reagent container, thereby calculating the cross-sectional area of each reagent container,

And correcting the effective test number of each reagent container according to the calculated sectional area, and registering the reagent pair again.

7. The automated analysis method of claim 6, wherein,

Calculating the deviation rate of the effective test number of the reagent to the registration and the actual consumption of the reagent as an error rate,

And correcting the effective test number according to the error rate.

8. The automated analysis method of claim 6, wherein,

After the start of the analysis, the number of effective tests per one of the reagent containers was corrected in accordance with the number of analysis times reduced by the software count, and the reagent pair was registered again.

9. The automated analysis method of claim 6, wherein,

The number of analyses until the number of effective tests is corrected is set in advance.

10. The automated analysis method of claim 6, wherein,

When the structure of the reagent pair is changed by the re-registration of the reagent pair, a notification is made that the change has occurred.