CN113717916B - Preparation method and application of composite microbial inoculum for treating preserved fruit wastewater - Google Patents
Preparation method and application of composite microbial inoculum for treating preserved fruit wastewater Download PDFInfo
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Abstract
The invention relates to the technical field of wastewater treatment, in particular to a preparation method and application of a composite microbial inoculum for treating preserved fruit wastewater, which comprises the steps of inoculating a salt-tolerant composite microbial inoculum into activated sludge according to a volume ratio of 3-10%; ammonia nitrogen and nitrite nitrogen are added into the activated sludge, and the ammonia nitrogen concentration in the activated sludge is gradually increased; domesticating the activated sludge under the anoxic-aerobic alternating condition to obtain domesticated sludge; the method comprises the steps of completely replacing domesticated water distribution of domesticated sludge by using wastewater to obtain a composite microbial inoculant suitable for treating Chinese-cherry wastewater, wherein the salt-tolerant composite microbial inoculant comprises denitrifying halomonas, bacillus subtilis, bacillus sphaericus, tetracoccus halophilus and bacillus marinus according to a mass ratio of 2-10:1-4:1-3:1-4:1-3, organic matters, ammonia nitrogen and total nitrogen in the waste liquid are effectively removed, and the impact resistance is stronger than that of a single strain, so that the problem that the traditional biological treatment technology is difficult to bear high salinity and poor in impact resistance in the preserved fruit waste water is solved.
Description
Technical Field
The invention relates to the technical field of wastewater treatment, in particular to a preparation method and application of a composite microbial inoculum for treating preserved fruit wastewater.
Background
The preserved fruit is a pickled food taking fresh fruits as main raw materials, and is popular with the masses in China. In the production process of preserved fruits, a large amount of wastewater with high osmotic pressure, high organic matter concentration and high ammonia nitrogen content can be produced by processes such as dipping, and the wastewater is mainly discharged after dilution by a production enterprise, so that the problems of eutrophication, blackening and stinking of a receiving water body, salinization and even hardening of surrounding soil and the like are easily caused, the ecological environment is seriously influenced, and the wastewater contains high-concentration salt, organic pollutants and ammonia nitrogen and belongs to one of the wastewater types which are extremely difficult to treat.
Problems encountered in the treatment of preserved fruit wastewater include the following two aspects: firstly, the preserved fruit wastewater contains high-concentration salt, organic pollutants and ammonia nitrogen, and belongs to one of the extremely difficult wastewater types. At present, the domestic treatment process for the preserved fruit wastewater is less in research, and focuses on the removal of salt, so that the attention to the removal of organic matters and ammonia nitrogen in the wastewater is insufficient. Secondly, the preserved fruit wastewater is treated by a physical and chemical method, so that the method has the advantages of large occupied area, large investment and high operation cost, and causes a certain economic burden to production enterprises. The biological method has good treatment effect and low operation cost, but the salt with too high concentration in the preserved fruit wastewater has toxic effect on common microorganisms, and the traditional biological treatment technology is difficult to directly treat the preserved fruit wastewater. Therefore, development of an economic, feasible, simple and effective preserved fruit wastewater treatment process is urgently needed.
Disclosure of Invention
The invention aims to provide a preparation method and application of a composite microbial inoculum for treating preserved fruit wastewater, and aims to solve the problems that the traditional biological treatment technology is difficult to bear high salinity and poor in impact resistance in the preserved fruit wastewater.
In order to achieve the above object, in a first aspect, the present invention provides a method for preparing a composite microbial inoculum for treating preserved fruit wastewater, comprising:
Inoculating the salt-tolerant composite microbial inoculum into the activated sludge according to the volume ratio of 3-10%;
ammonia nitrogen and nitrite nitrogen are added into the activated sludge, and the ammonia nitrogen concentration in the activated sludge is gradually increased;
Domesticating the activated sludge under the anoxic-aerobic alternating condition to obtain domesticated sludge;
And completely replacing acclimatized water distribution of the acclimatized sludge by using wastewater to obtain a composite microbial inoculum suitable for treating the preserved fruit wastewater.
Wherein the salt-tolerant composite microbial inoculum is prepared from denitrifying halomonas, bacillus subtilis, bacillus sphaericus, tetracoccus halophilus and bacillus marinus according to the mass ratio of 2-10:1-4:1-3:1-4: 1-3.
Wherein the concentration ratio of the ammonia nitrogen to the nitrite nitrogen is 1:1-1:1.5.
Wherein the domestication period of the domestication under the anoxic-aerobic alternating condition is 10-24 hours.
In a second aspect, the invention provides an application of a composite microbial inoculant for treating preserved fruit wastewater, comprising:
Filtering the preserved fruit wastewater to obtain filtered wastewater;
adding sodium hydroxide into the filtered wastewater to stabilize the pH value of the filtered wastewater at 6-8, thereby obtaining pretreated wastewater;
inoculating a composite microbial inoculum for treating the preserved fruit wastewater into the pretreated wastewater, and performing anoxic-aerobic alternate treatment to obtain biological treatment wastewater;
treating ammonia nitrogen and refractory organic matters in the biological treatment wastewater by using a membrane bioreactor to obtain degradation wastewater;
And removing salt and dissolved inorganic matters in the degradation wastewater by utilizing a desalting device.
Wherein the inoculation amount of the composite microbial inoculum for treating the preserved fruit wastewater is 1500-4000mg/L.
Wherein, the desalting device is any one or a combination of a plurality of membrane separation, electrodialysis and MVR.
The invention relates to a preparation method and application of a composite microbial inoculum for treating preserved fruit wastewater, which comprises the steps of inoculating a salt-resistant composite microbial inoculum into activated sludge according to a volume ratio of 3-10%; ammonia nitrogen and nitrite nitrogen are added into the activated sludge, and the ammonia nitrogen concentration in the activated sludge is gradually increased; domesticating the activated sludge under the anoxic-aerobic alternating condition to obtain domesticated sludge; the domesticated water distribution of the domesticated sludge is completely replaced by the wastewater to obtain a composite microbial agent suitable for treating the preserved fruit wastewater, the salt-resistant composite microbial agent consists of a plurality of bacterial strains, and the high-efficiency treatment of the preserved fruit wastewater can be realized under the high-salt condition under the synergistic effect of a plurality of microorganisms. Organic matters, ammonia nitrogen and total nitrogen in the waste liquid are effectively removed, and the impact resistance is stronger than that of a single strain, so that the problem that the traditional biological treatment technology is difficult to bear high salinity and poor in impact resistance in the preserved fruit waste water is solved.
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In order to more clearly illustrate the embodiments of the invention or the technical solutions in the prior art, the drawings that are required in the embodiments or the description of the prior art will be briefly described, it being obvious that the drawings in the following description are only some embodiments of the invention, and that other drawings may be obtained according to these drawings without inventive effort for a person skilled in the art.
FIG. 1 is a flow chart of a preparation method of a composite microbial inoculant for treating preserved fruit wastewater, provided by the invention;
FIG. 2 is a flow chart of application of the composite microbial inoculum for treating preserved fruit wastewater.
Detailed Description
Embodiments of the present invention are described in detail below, examples of which are illustrated in the accompanying drawings, wherein like or similar reference numerals refer to like or similar elements or elements having like or similar functions throughout. The embodiments described below by referring to the drawings are illustrative and intended to explain the present invention and should not be construed as limiting the invention.
Referring to fig. 1, in a first aspect, the present invention provides a method for preparing a composite microbial inoculant for treating preserved fruit waste water, comprising:
s101, inoculating a salt-tolerant composite microbial inoculum into activated sludge according to the volume ratio of 3-10%;
The salt-tolerant composite microbial inoculum is prepared from denitrifying halomonas, bacillus subtilis, bacillus sphaericus, tetracoccus halophilus and bacillus marinus according to the mass ratio of 2-10:1-4:1-3:1-4: 1-3.
S102, adding ammonia nitrogen and nitrite nitrogen into the activated sludge, and gradually increasing the ammonia nitrogen concentration in the activated sludge;
Stabilizing the pH value in the activated sludge at 6-8, wherein the activated sludge takes carbon-containing compounds as carbon sources, and the concentration ratio of the input ammonia nitrogen to the nitrite nitrogen is 1:1-1:1.5, controlling the ammonia nitrogen concentration in the activated sludge by adopting a gradient concentration method, wherein the gradient concentration method comprises the following steps: the initial ammonia nitrogen concentration is 20-50mg/L, the water effect is seen by lifting the ammonia nitrogen concentration by 20-50mg/L each time, the discharged water is removed by more than 85%, the adding amount can be increased 1-3 days after the discharged water is stabilized, and finally the adding amount can be increased to 120-150mg/L.
S103, domesticating the activated sludge under the anoxic-aerobic alternate condition to obtain domesticated sludge;
the domestication period of the domestication under the anoxic-aerobic alternating condition is 10-24 hours until the salinity tolerance reaches 5%.
S104, completely replacing acclimatized water distribution of the acclimatized sludge by using the wastewater to obtain the composite microbial inoculum suitable for treating the preserved fruit wastewater.
The actual wastewater is subjected to batch acclimation and water distribution of the acclimation sludge replacement according to the sequence of 1/5, 1/4, 1/2 and 1/1 of the water inflow until the wastewater is completely replaced by the acclimation and water distribution.
Referring to fig. 2, in a second aspect, the present invention provides an application of a composite microbial inoculant for treating preserved fruit waste water, including:
S201, filtering the preserved fruit wastewater to obtain filtered wastewater;
removing water impurities and insoluble substances during filtration.
S202, adding sodium hydroxide into the filtered wastewater to stabilize the pH value of the filtered wastewater at 6-8, thereby obtaining pretreated wastewater;
the activity of the microorganism is higher when the pH value is stabilized at 6-8.
S203, inoculating a composite microbial inoculum suitable for treating the preserved fruit wastewater into the pretreated wastewater, and performing anoxic-aerobic alternate treatment to obtain biological treatment wastewater;
The pretreated wastewater needs to be fully contacted and reacted with the composite microbial agent for treating the preserved fruit wastewater, and the composite microbial agent for treating the preserved fruit wastewater has the synergistic effect of a plurality of microorganisms, so that the organic matters, ammonia nitrogen and total nitrogen in the preserved fruit wastewater can be efficiently removed under the conditions of aerobics and high salt, and the inoculation amount of the composite microbial agent for treating the preserved fruit wastewater is 1500-4000mg/L.
S204, treating ammonia nitrogen and refractory organic matters in the biological treatment wastewater by using a membrane bioreactor to obtain degradation wastewater;
The effluent quality of the biological treatment wastewater treated by the membrane bioreactor is stable, and suspended matters and turbidity in water are reduced.
S205, removing salt and dissolved inorganic matters in the degradation wastewater by utilizing a desalting device.
The desalting device is any one or a combination of a plurality of membrane separation, electrodialysis and MVR.
The invention relates to a preparation method and application of a composite microbial inoculum for treating preserved fruit wastewater, which comprises the steps of inoculating a salt-resistant composite microbial inoculum into activated sludge according to a volume ratio of 3-10%; ammonia nitrogen and nitrite nitrogen are added into the activated sludge, and the ammonia nitrogen concentration in the activated sludge is gradually increased; domesticating the activated sludge under the anoxic-aerobic alternating condition to obtain domesticated sludge; the domesticated water distribution of the domesticated sludge is completely replaced by the wastewater to obtain a composite microbial agent suitable for treating the preserved fruit wastewater, the salt-resistant composite microbial agent consists of a plurality of bacterial strains, and the high-efficiency treatment of the preserved fruit wastewater can be realized under the high-salt condition under the synergistic effect of a plurality of microorganisms. Organic matters, ammonia nitrogen and total nitrogen in the waste liquid are effectively removed, and the impact resistance is stronger than that of a single strain, so that the problem that the traditional biological treatment technology is difficult to bear high salinity and poor in impact resistance in the preserved fruit waste water is solved.
Example 1:
the salt-tolerant composite microbial inoculum consists of denitrifying halomonas, bacillus subtilis, bacillus sphaericus, tetracoccus halophilus and bacillus marinus according to the mass ratio of 5:4:3:2:2.
Inoculating 10% of the salt-tolerant composite microbial inoculum into common activated sludge with the MLSS concentration of 3000mg/L, and adding the mixture with the concentration ratio of 1:1.5, domesticating ammonia nitrogen and nitrite nitrogen under the anoxic-aerobic alternating condition with a period of 18 hours by adopting a gradient concentration method until the salinity tolerance reaches 5%, and replacing the domesticated sludge with the actual wastewater in batches according to the sequence of 1/5, 1/4, 1/2 and 1/1 of the water inflow until the wastewater is completely replaced by the domesticated water distribution, thereby obtaining the composite microbial inoculum for treating the Chinese olive wastewater. The initial COD of the preserved fruit wastewater is about 5000mg/L, the ammonia nitrogen is about 100mg/L, and the salinity is 4.8%. Treating the waste water under the anaerobic-aerobic alternating conditions with the pH value of 7 and the dissolved oxygen of 0.08-4.2 mg/L for 10-24 hours, inoculating the composite microbial inoculum for treating the preserved fruit waste water for 3 days, wherein the effluent quality meets the starting requirement, the COD removal rate is 90%, the denitrification rate is 94-96%, and the membrane bioreactor is used for treating ammonia nitrogen and refractory organic matters in the biological treatment waste water to obtain degraded waste water; and (5) removing salt and dissolved inorganic matters in the degradation wastewater by using a desalting device, and completing wastewater treatment.
The above disclosure is only illustrative of a preparation method and application of a composite microbial agent for treating fruit waste water, but the scope of the invention is not limited thereto, and those skilled in the art will understand that all or part of the procedures for implementing the above embodiments are equivalent and still fall within the scope of the invention.
Claims (6)
1. The preparation method of the composite microbial inoculum for treating the preserved fruit wastewater is characterized by comprising the following steps of:
Inoculating the salt-tolerant composite microbial inoculum into the activated sludge according to the volume ratio of 3-10%;
ammonia nitrogen and nitrite nitrogen are added into the activated sludge, and the ammonia nitrogen concentration in the activated sludge is gradually increased;
Domesticating the activated sludge under the anoxic-aerobic alternating condition to obtain domesticated sludge;
completely replacing acclimatized water distribution of acclimatized sludge by using wastewater to obtain a composite microbial inoculum suitable for treating preserved fruit wastewater;
The salt-tolerant composite microbial inoculum consists of denitrifying halomonas (Halomonas denitrificans), bacillus subtilis (Bacillus subtilis), bacillus sphaericus (Bacillus sphaericus), tetracoccus halophilus (Tetragenococcus halophilus) and bacillus marinus (Marinobacter) according to a mass ratio of 5:4:3:2:2.
2. The method for preparing the composite microbial inoculum for treating the Chinese herbal fruit wastewater according to claim 1, which is characterized in that,
The concentration ratio of the ammonia nitrogen to the nitrite nitrogen is 1:1-1:1.5.
3. The method for preparing the composite microbial inoculum for treating the Chinese herbal fruit wastewater according to claim 1, which is characterized in that,
The domestication period of the domestication under the anoxic-aerobic alternating condition is 10-24 hours.
4. The application of the composite microbial inoculum prepared by the method of claim 1 for treating preserved fruit wastewater is characterized by comprising the following steps:
Filtering the preserved fruit wastewater to obtain filtered wastewater;
adding sodium hydroxide into the filtered wastewater to stabilize the pH value of the filtered wastewater at 6-8, thereby obtaining pretreated wastewater;
inoculating a composite microbial inoculum for treating the preserved fruit wastewater into the pretreated wastewater, and performing anoxic-aerobic alternate treatment to obtain biological treatment wastewater;
treating ammonia nitrogen and refractory organic matters in the biological treatment wastewater by using a membrane bioreactor to obtain degradation wastewater;
And removing salt and dissolved inorganic matters in the degradation wastewater by utilizing a desalting device.
5. The use according to claim 4, wherein,
The inoculation amount of the composite microbial inoculum for treating the preserved fruit wastewater is 1500-4000mg/L.
6. The use according to claim 4, wherein,
The desalting device is any one or a combination of a plurality of membrane separation, electrodialysis and MVR.
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| CN105198178A (en) * | 2015-11-17 | 2015-12-30 | 永仁新得天生物科技有限公司 | Method for treating small preserved tomato processing wastewater |
| CN105712572A (en) * | 2015-12-04 | 2016-06-29 | 韩山师范学院 | Method for treating preserved fruit wastewater on basis of membrane integration technologies |
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| JP3089274B1 (en) * | 1999-03-29 | 2000-09-18 | 学校法人立命館 | Salt Tolerant Staphylococcus sp. CH-4 and Method for Treating High Salt Concentration Waste Liquid |
| WO2001034523A1 (en) * | 1999-11-06 | 2001-05-17 | Stephen Ray Wurzburger | Treatment of contaminated waste water |
| GR1004115B (en) * | 2002-04-30 | 2003-01-21 | Combined biotechnological and chemical method of treating liquid wastes derived from factories producing edible olives | |
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| CN105198178A (en) * | 2015-11-17 | 2015-12-30 | 永仁新得天生物科技有限公司 | Method for treating small preserved tomato processing wastewater |
| CN105712572A (en) * | 2015-12-04 | 2016-06-29 | 韩山师范学院 | Method for treating preserved fruit wastewater on basis of membrane integration technologies |
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