CN113717916A - Preparation method and application of complex microbial inoculant for treating preserved fruit wastewater - Google Patents
Preparation method and application of complex microbial inoculant for treating preserved fruit wastewater Download PDFInfo
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- 239000002351 wastewater Substances 0.000 title claims abstract description 100
- 235000013399 edible fruits Nutrition 0.000 title claims abstract description 54
- 238000002360 preparation method Methods 0.000 title claims abstract description 11
- 230000000813 microbial effect Effects 0.000 title claims description 10
- 239000002054 inoculum Substances 0.000 title claims description 7
- 239000002068 microbial inoculum Substances 0.000 claims abstract description 42
- 239000010802 sludge Substances 0.000 claims abstract description 42
- XKMRRTOUMJRJIA-UHFFFAOYSA-N ammonia nh3 Chemical compound N.N XKMRRTOUMJRJIA-UHFFFAOYSA-N 0.000 claims abstract description 32
- 239000002131 composite material Substances 0.000 claims abstract description 26
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 16
- JVMRPSJZNHXORP-UHFFFAOYSA-N ON=O.ON=O.ON=O.N Chemical compound ON=O.ON=O.ON=O.N JVMRPSJZNHXORP-UHFFFAOYSA-N 0.000 claims abstract description 10
- 238000009826 distribution Methods 0.000 claims abstract description 10
- 244000063299 Bacillus subtilis Species 0.000 claims abstract description 5
- 235000014469 Bacillus subtilis Nutrition 0.000 claims abstract description 5
- 241000206596 Halomonas Species 0.000 claims abstract description 5
- 241000193386 Lysinibacillus sphaericus Species 0.000 claims abstract description 5
- 241001537924 Tetracoccus <angiosperm> Species 0.000 claims abstract description 4
- 238000001816 cooling Methods 0.000 claims abstract 5
- 238000000034 method Methods 0.000 claims description 11
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 claims description 9
- 239000012528 membrane Substances 0.000 claims description 8
- 150000003839 salts Chemical class 0.000 claims description 8
- 238000011033 desalting Methods 0.000 claims description 7
- 238000001914 filtration Methods 0.000 claims description 4
- 239000003795 chemical substances by application Substances 0.000 claims description 3
- 238000000909 electrodialysis Methods 0.000 claims description 3
- 238000011081 inoculation Methods 0.000 claims description 3
- 238000000926 separation method Methods 0.000 claims description 3
- 230000015556 catabolic process Effects 0.000 claims description 2
- 238000006731 degradation reaction Methods 0.000 claims description 2
- 235000015110 jellies Nutrition 0.000 claims 2
- 239000008274 jelly Substances 0.000 claims 2
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 abstract description 8
- 238000005516 engineering process Methods 0.000 abstract description 5
- 229910052757 nitrogen Inorganic materials 0.000 abstract description 4
- 238000004065 wastewater treatment Methods 0.000 abstract description 4
- 239000007788 liquid Substances 0.000 abstract description 3
- 239000002699 waste material Substances 0.000 abstract description 3
- 241000206589 Marinobacter Species 0.000 abstract description 2
- 244000005700 microbiome Species 0.000 description 5
- 150000001875 compounds Chemical class 0.000 description 4
- 230000000694 effects Effects 0.000 description 3
- 238000004519 manufacturing process Methods 0.000 description 3
- 239000005416 organic matter Substances 0.000 description 3
- 230000002195 synergetic effect Effects 0.000 description 3
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 description 2
- 230000001580 bacterial effect Effects 0.000 description 2
- 229910052799 carbon Inorganic materials 0.000 description 2
- 239000002957 persistent organic pollutant Substances 0.000 description 2
- 241000500332 Tetragenococcus halophilus Species 0.000 description 1
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 1
- 238000010170 biological method Methods 0.000 description 1
- 238000006243 chemical reaction Methods 0.000 description 1
- 238000010790 dilution Methods 0.000 description 1
- 239000012895 dilution Substances 0.000 description 1
- 238000007598 dipping method Methods 0.000 description 1
- 238000012851 eutrophication Methods 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- 235000021022 fresh fruits Nutrition 0.000 description 1
- 239000012535 impurity Substances 0.000 description 1
- 230000003204 osmotic effect Effects 0.000 description 1
- 229910052760 oxygen Inorganic materials 0.000 description 1
- 239000001301 oxygen Substances 0.000 description 1
- 230000002093 peripheral effect Effects 0.000 description 1
- 238000011197 physicochemical method Methods 0.000 description 1
- 239000002994 raw material Substances 0.000 description 1
- 239000002689 soil Substances 0.000 description 1
- 239000000243 solution Substances 0.000 description 1
- 230000000087 stabilizing effect Effects 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 231100000331 toxic Toxicity 0.000 description 1
- 230000002588 toxic effect Effects 0.000 description 1
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/36—Adaptation or attenuation of cells
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F9/00—Multistage treatment of water, waste water or sewage
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F1/00—Treatment of water, waste water, or sewage
- C02F1/001—Processes for the treatment of water whereby the filtration technique is of importance
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- C02F1/00—Treatment of water, waste water, or sewage
- C02F1/44—Treatment of water, waste water, or sewage by dialysis, osmosis or reverse osmosis
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- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F1/00—Treatment of water, waste water, or sewage
- C02F1/46—Treatment of water, waste water, or sewage by electrochemical methods
- C02F1/469—Treatment of water, waste water, or sewage by electrochemical methods by electrochemical separation, e.g. by electro-osmosis, electrodialysis, electrophoresis
- C02F1/4693—Treatment of water, waste water, or sewage by electrochemical methods by electrochemical separation, e.g. by electro-osmosis, electrodialysis, electrophoresis electrodialysis
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- C02F1/66—Treatment of water, waste water, or sewage by neutralisation; pH adjustment
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- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2101/00—Nature of the contaminant
- C02F2101/10—Inorganic compounds
- C02F2101/16—Nitrogen compounds, e.g. ammonia
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- C02F2101/166—Nitrites
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Abstract
The invention relates to the technical field of wastewater treatment, in particular to a preparation method and application of a complex microbial inoculum for treating cold fruit wastewater, wherein a salt-tolerant complex microbial inoculum is inoculated into activated sludge according to the volume ratio of 3-10%; adding ammonia nitrogen and nitrite nitrogen into the activated sludge, and gradually increasing the ammonia nitrogen concentration in the activated sludge; acclimating the activated sludge under an anoxic-aerobic alternative condition to obtain acclimated sludge; completely replacing domesticated water distribution of domesticated sludge by using wastewater to obtain a composite microbial inoculum suitable for treating the fruit cooling wastewater, wherein the salt-tolerant composite microbial inoculum is prepared from denitrifying halomonas, bacillus subtilis, bacillus sphaericus, halophilic tetracoccus and marinobacter according to the mass ratio of 2-10: 1-4: 1-3: 1-4: 1-3, effectively removes organic matters, ammonia nitrogen and total nitrogen in the waste liquid, has stronger impact resistance than a single strain, and solves the problems that the traditional biological treatment technology is difficult to bear high salinity and poor impact resistance in the preserved fruit waste water.
Description
Technical Field
The invention relates to the technical field of wastewater treatment, in particular to a preparation method and application of a complex microbial inoculum for treating preserved fruit wastewater.
Background
Preserved fruit is a pickled food which takes fresh fruit as main raw material and is popular among the people in China. In the production process of the preserved fruits, a large amount of wastewater with high osmotic pressure, high organic matter concentration and high ammonia nitrogen content is generated by processes such as dipping and the like, and the wastewater is mainly treated by production enterprises for dilution and then discharged, so that the problems of eutrophication, blackening and smelling of a receiving water body, salinization and even hardening of peripheral soil and the like are easily caused, the ecological environment is seriously influenced, and the preserved fruits contain high-concentration salt, organic pollutants and ammonia nitrogen and belong to one of the types of wastewater which is extremely difficult to treat.
The problems encountered in the treatment of the preserved fruit wastewater include the following two aspects: firstly, the preserved fruit wastewater contains high-concentration salt, organic pollutants and ammonia nitrogen, and belongs to one of the wastewater types which are extremely difficult to treat. At present, the domestic treatment process for the preserved fruit wastewater is less researched, the emphasis is on removing the salt, and the attention on removing the organic matters and ammonia nitrogen in the wastewater is not enough. Secondly, the physicochemical method is adopted to treat the cold fruit wastewater, which not only occupies large area, has more investment and high operating cost, but also causes certain economic burden to production enterprises. The biological method has good treatment effect and low operation cost, but the salt with too high concentration in the cold fruit wastewater has toxic action on common microorganisms, and the traditional biological treatment technology is often difficult to directly treat the cold fruit wastewater. Therefore, the development of an economic, feasible, simple and effective preserved fruit wastewater treatment process is urgently needed.
Disclosure of Invention
The invention aims to provide a preparation method and application of a complex microbial inoculant for treating preserved fruit wastewater, and aims to solve the problems that the traditional biological treatment technology cannot bear high salinity and poor impact resistance in the preserved fruit wastewater.
In order to achieve the above object, in a first aspect, the present invention provides a method for preparing a complex microbial inoculant for treating cold fruit wastewater, comprising:
inoculating the salt-tolerant composite microbial inoculum into the activated sludge according to the volume ratio of 3-10%;
adding ammonia nitrogen and nitrite nitrogen into the activated sludge, and gradually increasing the ammonia nitrogen concentration in the activated sludge;
acclimating the activated sludge under an anoxic-aerobic alternative condition to obtain acclimated sludge;
and (3) completely replacing domesticated water distribution of the domesticated sludge by using the wastewater to obtain the composite microbial inoculum suitable for treating the preserved fruit wastewater.
Wherein the salt-tolerant composite microbial inoculum is prepared from denitrifying halomonas, bacillus subtilis, bacillus sphaericus, tetragenococcus halophilus and marinobacter according to the mass ratio of 2-10: 1-4: 1-3: 1-4: 1-3.
Wherein the concentration ratio of the ammonia nitrogen to the nitrite nitrogen is 1: 1-1: 1.5.
wherein the acclimation period for acclimation under the anaerobic-aerobic alternate condition is 10-24 hours.
In a second aspect, the invention provides an application of a composite microbial inoculum for treating cold fruit wastewater, which comprises the following steps:
filtering the preserved fruit wastewater to obtain filtered wastewater;
adding sodium hydroxide into the filtered wastewater to stabilize the pH value of the filtered wastewater to 6-8, thereby obtaining pretreated wastewater;
inoculating a composite microbial inoculum for treating the preserved fruit wastewater into the pretreated wastewater, and performing anoxic-aerobic alternate treatment to obtain biological treatment wastewater;
treating ammonia nitrogen and refractory organics in the biological treatment wastewater by using a membrane bioreactor to obtain degradation wastewater;
and removing salt and dissolved inorganic matters in the degraded wastewater by using a desalting device.
Wherein the inoculation amount of the composite microbial inoculum for treating the cold fruit wastewater is 1500-4000 mg/L.
Wherein the desalting device is any one or combination of membrane separation, electrodialysis and MVR.
The preparation method and the application of the complex microbial inoculum for treating the preserved fruit wastewater are characterized in that the salt-tolerant complex microbial inoculum is inoculated into activated sludge according to the volume ratio of 3-10%; adding ammonia nitrogen and nitrite nitrogen into the activated sludge, and gradually increasing the ammonia nitrogen concentration in the activated sludge; acclimating the activated sludge under an anoxic-aerobic alternative condition to obtain acclimated sludge; the domesticated water distribution of the domesticated sludge is completely replaced by the wastewater to obtain the composite microbial inoculum suitable for treating the preserved fruit wastewater, the salt-tolerant composite microbial inoculum consists of a plurality of bacterial strains, and the high-efficiency treatment of the preserved fruit wastewater can be realized under the high-salt condition under the synergistic effect of a plurality of microorganisms. The organic matter, ammonia nitrogen and total nitrogen in the waste liquid are effectively removed, the impact resistance is stronger than that of a single strain, and the problems that the traditional biological treatment technology is difficult to bear high salinity and poor impact resistance in the preserved fruit waste water are solved.
Drawings
In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings used in the description of the embodiments or the prior art will be briefly described below, it is obvious that the drawings in the following description are only some embodiments of the present invention, and for those skilled in the art, other drawings can be obtained according to the drawings without creative efforts.
FIG. 1 is a flow chart of a preparation method of a complex microbial inoculum for treating cold fruit wastewater provided by the invention;
FIG. 2 is a flow chart of the application of the complex microbial inoculum for treating the preserved fruit wastewater provided by the invention.
Detailed Description
Reference will now be made in detail to embodiments of the present invention, examples of which are illustrated in the accompanying drawings, wherein like or similar reference numerals refer to the same or similar elements or elements having the same or similar function throughout. The embodiments described below with reference to the drawings are illustrative and intended to be illustrative of the invention and are not to be construed as limiting the invention.
Referring to fig. 1, in a first aspect, the present invention provides a method for preparing a complex microbial inoculum for treating cold fruit wastewater, comprising:
s101, inoculating the salt-tolerant complex microbial inoculum into activated sludge according to the volume ratio of 3-10%;
the salt-tolerant composite microbial agent is prepared from denitrifying halomonas, bacillus subtilis, bacillus sphaericus, halophilic tetracoccus and marinus according to the mass ratio of 2-10: 1-4: 1-3: 1-4: 1-3.
S102, adding ammonia nitrogen and nitrite nitrogen into the activated sludge, and gradually increasing the ammonia nitrogen concentration in the activated sludge;
stabilizing the pH value of the activated sludge at 6-8, wherein the activated sludge takes carbon-containing compounds as carbon sources, and the concentration ratio of the added ammonia nitrogen to the nitrite nitrogen is 1: 1-1: 1.5, controlling the ammonia nitrogen concentration in the activated sludge by adopting a gradient concentration method, wherein the gradient concentration method comprises the following steps: the initial ammonia nitrogen concentration is 20-50mg/L, the ammonia nitrogen concentration is increased by 20-50mg/L each time, the water effect is seen by increasing the frequency, more than 85% of the effluent is removed, the dosage can be increased after 1-3 days after the effluent is stable, and finally the dosage can be increased to 120-150 mg/L.
S103, acclimatizing the activated sludge under an anoxic-aerobic alternative condition to obtain acclimatized sludge;
the acclimation period for acclimation under the anaerobic-aerobic alternate condition is 10-24 hours until the salinity tolerance reaches 5%.
S104, completely replacing domesticated water distribution of the domesticated sludge by using the wastewater to obtain the composite microbial inoculum suitable for treating the preserved fruit wastewater.
And replacing domesticated water distribution of domesticated sludge in batches according to the sequence of water inflow of 1/5, 1/4, 1/2 and 1/1 until the domesticated water distribution is completely replaced by the wastewater.
Referring to fig. 2, in a second aspect, the present invention provides an application of a complex microbial inoculant for treating cold fruit wastewater, comprising:
s201, filtering the preserved fruit wastewater to obtain filtered wastewater;
removing impurities and insoluble substances in water during filtration.
S202, adding sodium hydroxide into the filtered wastewater to stabilize the pH value of the filtered wastewater to 6-8 to obtain pretreated wastewater;
when the pH value is stabilized at 6-8, the activity of the microorganism is higher.
S203, inoculating a composite microbial inoculum suitable for treating the preserved fruit wastewater into the pretreated wastewater, and performing anoxic-aerobic alternate treatment to obtain biological treatment wastewater;
the pretreatment wastewater needs to be in full contact reaction with the compound microbial inoculum for treating the preserved fruit wastewater, the synergistic effect of various microorganisms in the compound microbial inoculum for treating the preserved fruit wastewater can realize the efficient removal of organic matters, ammonia nitrogen and total nitrogen in the preserved fruit wastewater under the aerobic and high-salt conditions, and the inoculation amount of the compound microbial inoculum for treating the preserved fruit wastewater is 1500-4000 mg/L.
S204, treating ammonia nitrogen and refractory organic matters in the biological treatment wastewater by using a membrane bioreactor to obtain degraded wastewater;
the effluent quality of the biological treatment wastewater treated by the membrane bioreactor is stable, and suspended matters and turbidity in the water are reduced.
S205, removing salt and dissolved inorganic matters in the degraded wastewater by using a desalting device.
The desalting device is any one or combination of membrane separation, electrodialysis and MVR.
The preparation method and the application of the complex microbial inoculum for treating the preserved fruit wastewater are characterized in that the salt-tolerant complex microbial inoculum is inoculated into activated sludge according to the volume ratio of 3-10%; adding ammonia nitrogen and nitrite nitrogen into the activated sludge, and gradually increasing the ammonia nitrogen concentration in the activated sludge; acclimating the activated sludge under an anoxic-aerobic alternative condition to obtain acclimated sludge; the domesticated water distribution of the domesticated sludge is completely replaced by the wastewater to obtain the composite microbial inoculum suitable for treating the preserved fruit wastewater, the salt-tolerant composite microbial inoculum consists of a plurality of bacterial strains, and the high-efficiency treatment of the preserved fruit wastewater can be realized under the high-salt condition under the synergistic effect of a plurality of microorganisms. The organic matter, ammonia nitrogen and total nitrogen in the waste liquid are effectively removed, the impact resistance is stronger than that of a single strain, and the problems that the traditional biological treatment technology is difficult to bear high salinity and poor impact resistance in the preserved fruit waste water are solved.
Example 1:
the salt-tolerant composite microbial agent consists of denitrifying halomonas, bacillus subtilis, bacillus sphaericus, halophilic tetracoccus and marinus according to the mass ratio of 5:4:3:2: 2.
Inoculating the salt-tolerant complex microbial inoculum with the volume ratio of 10% into common activated sludge with the MLSS concentration of 3000mg/L, and adding the salt-tolerant complex microbial inoculum with the concentration ratio of 1: 1.5, acclimatizing the ammonia nitrogen and nitrite nitrogen under an anoxic-aerobic alternative condition with a period of 18h by adopting a gradient concentration method until the salinity tolerance reaches 5%, replacing acclimatized water distribution of acclimatized sludge in batches according to the sequence of water inflow 1/5, 1/4, 1/2 and 1/1 until the acclimatized water distribution is completely replaced by the wastewater, and obtaining the composite microbial inoculum for treating the cold fruit wastewater. The original COD of the preserved fruit wastewater is about 5000mg/L, the ammonia nitrogen is about 100mg/L, and the salinity is 4.8%. Treating the waste water under an anoxic-aerobic alternative condition with the pH of 7 and the dissolved oxygen of 0.08-4.2 mg/L, wherein the treatment period is 10-24 h, the composite microbial inoculum for treating the preserved fruit waste water is inoculated for 3 days, the effluent quality meets the starting requirement, the COD removal rate is 90%, the denitrification rate reaches 94-96%, and ammonia nitrogen and refractory organic matters in the biological treatment waste water are treated by a membrane bioreactor to obtain degraded waste water; and (5) removing salt and dissolved inorganic matters in the degraded wastewater by using a desalting device and finishing wastewater treatment.
The above disclosure is only a preferred embodiment of the present invention, and certainly does not limit the scope of the present invention, and persons skilled in the art can understand that all or part of the procedures of the above embodiment can be implemented and equivalents thereof made according to the claims of the present invention, and still fall within the scope of the present invention.
Claims (7)
1. A preparation method of a composite microbial inoculum for treating cold fruit wastewater is characterized by comprising the following steps:
inoculating the salt-tolerant composite microbial inoculum into the activated sludge according to the volume ratio of 3-10%;
adding ammonia nitrogen and nitrite nitrogen into the activated sludge, and gradually increasing the ammonia nitrogen concentration in the activated sludge;
acclimating the activated sludge under an anoxic-aerobic alternative condition to obtain acclimated sludge;
and (3) completely replacing domesticated water distribution of the domesticated sludge by using the wastewater to obtain the composite microbial inoculum suitable for treating the preserved fruit wastewater.
2. The preparation method of the complex microbial inoculum for treating the fruit-cooling wastewater as claimed in claim 1, which comprises the following steps:
the salt-tolerant composite microbial agent is prepared from denitrifying halomonas, bacillus subtilis, bacillus sphaericus, halophilic tetracoccus and marinus according to the mass ratio of 2-10: 1-4: 1-3: 1-4: 1-3.
3. The method for preparing complex microbial inoculum for treating fruit-cooling wastewater as claimed in claim 1,
the concentration ratio of the ammonia nitrogen to the nitrite nitrogen is 1: 1-1: 1.5.
4. the method for preparing complex microbial inoculum for treating fruit-cooling wastewater as claimed in claim 1,
the acclimation period for acclimation under the anaerobic-aerobic alternate condition is 10-24 hours.
5. The application of the composite microbial inoculum for treating the fruit jelly wastewater in the preparation method of the composite microbial inoculum for treating the fruit jelly wastewater in claim 1 is characterized by comprising the following steps:
filtering the preserved fruit wastewater to obtain filtered wastewater;
adding sodium hydroxide into the filtered wastewater to stabilize the pH value of the filtered wastewater to 6-8, thereby obtaining pretreated wastewater;
inoculating a composite microbial inoculum for treating the preserved fruit wastewater into the pretreated wastewater, and performing anoxic-aerobic alternate treatment to obtain biological treatment wastewater;
treating ammonia nitrogen and refractory organics in the biological treatment wastewater by using a membrane bioreactor to obtain degradation wastewater;
and removing salt and dissolved inorganic matters in the degraded wastewater by using a desalting device.
6. The application of the complex microbial inoculant for treating cold fruit wastewater as claimed in claim 5, wherein the inoculation amount of the complex microbial inoculant for treating cold fruit wastewater is 1500-4000 mg/L.
7. The application of the composite microbial inoculum for treating the fruit-cooling wastewater as claimed in claim 5,
the desalting device is any one or combination of membrane separation, electrodialysis and MVR.
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