CN113687504A - Uniform lighting device for observing tiny objects and using method thereof - Google Patents
Uniform lighting device for observing tiny objects and using method thereof Download PDFInfo
- Publication number
- CN113687504A CN113687504A CN202111028904.1A CN202111028904A CN113687504A CN 113687504 A CN113687504 A CN 113687504A CN 202111028904 A CN202111028904 A CN 202111028904A CN 113687504 A CN113687504 A CN 113687504A
- Authority
- CN
- China
- Prior art keywords
- semi
- integrating sphere
- light source
- optical fiber
- light
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 238000000034 method Methods 0.000 title claims abstract description 14
- 238000005286 illumination Methods 0.000 claims abstract description 50
- 239000013307 optical fiber Substances 0.000 claims abstract description 38
- 229910000838 Al alloy Inorganic materials 0.000 claims description 4
- 239000011248 coating agent Substances 0.000 claims description 4
- 238000000576 coating method Methods 0.000 claims description 4
- 229910052736 halogen Inorganic materials 0.000 claims description 4
- 150000002367 halogens Chemical class 0.000 claims description 4
- -1 polytetrafluoroethylene Polymers 0.000 claims description 4
- 229920001343 polytetrafluoroethylene Polymers 0.000 claims description 4
- 239000004810 polytetrafluoroethylene Substances 0.000 claims description 4
- 229910052751 metal Inorganic materials 0.000 claims description 3
- 239000002184 metal Substances 0.000 claims description 3
- 229910052724 xenon Inorganic materials 0.000 claims description 2
- FHNFHKCVQCLJFQ-UHFFFAOYSA-N xenon atom Chemical compound [Xe] FHNFHKCVQCLJFQ-UHFFFAOYSA-N 0.000 claims description 2
- 238000003384 imaging method Methods 0.000 abstract description 12
- 238000000701 chemical imaging Methods 0.000 abstract description 4
- 238000004088 simulation Methods 0.000 description 5
- 238000002474 experimental method Methods 0.000 description 4
- 238000004364 calculation method Methods 0.000 description 3
- 238000010586 diagram Methods 0.000 description 3
- 238000004458 analytical method Methods 0.000 description 2
- 238000013461 design Methods 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 238000005259 measurement Methods 0.000 description 2
- 238000005070 sampling Methods 0.000 description 2
- 238000010521 absorption reaction Methods 0.000 description 1
- 230000006978 adaptation Effects 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 238000011156 evaluation Methods 0.000 description 1
- 239000000835 fiber Substances 0.000 description 1
- 239000011521 glass Substances 0.000 description 1
- 238000010438 heat treatment Methods 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 239000011148 porous material Substances 0.000 description 1
- 230000003595 spectral effect Effects 0.000 description 1
- 238000002834 transmittance Methods 0.000 description 1
- 238000011077 uniformity evaluation Methods 0.000 description 1
Images
Classifications
-
- G—PHYSICS
- G02—OPTICS
- G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
- G02B21/00—Microscopes
- G02B21/06—Means for illuminating specimens
-
- F—MECHANICAL ENGINEERING; LIGHTING; HEATING; WEAPONS; BLASTING
- F21—LIGHTING
- F21V—FUNCTIONAL FEATURES OR DETAILS OF LIGHTING DEVICES OR SYSTEMS THEREOF; STRUCTURAL COMBINATIONS OF LIGHTING DEVICES WITH OTHER ARTICLES, NOT OTHERWISE PROVIDED FOR
- F21V1/00—Shades for light sources, i.e. lampshades for table, floor, wall or ceiling lamps
-
- F—MECHANICAL ENGINEERING; LIGHTING; HEATING; WEAPONS; BLASTING
- F21—LIGHTING
- F21V—FUNCTIONAL FEATURES OR DETAILS OF LIGHTING DEVICES OR SYSTEMS THEREOF; STRUCTURAL COMBINATIONS OF LIGHTING DEVICES WITH OTHER ARTICLES, NOT OTHERWISE PROVIDED FOR
- F21V7/00—Reflectors for light sources
-
- F—MECHANICAL ENGINEERING; LIGHTING; HEATING; WEAPONS; BLASTING
- F21—LIGHTING
- F21V—FUNCTIONAL FEATURES OR DETAILS OF LIGHTING DEVICES OR SYSTEMS THEREOF; STRUCTURAL COMBINATIONS OF LIGHTING DEVICES WITH OTHER ARTICLES, NOT OTHERWISE PROVIDED FOR
- F21V2200/00—Use of light guides, e.g. fibre optic devices, in lighting devices or systems
- F21V2200/10—Use of light guides, e.g. fibre optic devices, in lighting devices or systems of light guides of the optical fibres type
- F21V2200/13—Use of light guides, e.g. fibre optic devices, in lighting devices or systems of light guides of the optical fibres type the light being emitted at the end of the guide
Landscapes
- Engineering & Computer Science (AREA)
- General Engineering & Computer Science (AREA)
- Physics & Mathematics (AREA)
- Chemical & Material Sciences (AREA)
- Analytical Chemistry (AREA)
- General Physics & Mathematics (AREA)
- Optics & Photonics (AREA)
- Microscoopes, Condenser (AREA)
Abstract
本发明公开了一种用于微小物体观测的均匀照明装置及其使用方法，本发明的装置包括半积分球和光纤冷光源，半积分球的壳壁上设有至少四个进光孔，在半积分球内部，每个进光孔对应地设有一个竖直的圆形遮光屏；光纤冷光源包括光源壳体，在光源壳体内部设有光源和平凸透镜，在光源壳体上设有一个光纤连接口，光纤的入射端与光纤连接口固定连接，光纤的出射端分别与进光孔固定连接且均伸入半积分球的内腔中正对圆形遮光屏。本发明用以给微小物体提供均匀光照环境的装置，满足体视显微镜的数码成像拍照、摄像或光谱成像观测的需要。
The invention discloses a uniform illumination device for observing tiny objects and a method of using the same. The device comprises a semi-integrating sphere and an optical fiber cold light source. The shell wall of the semi-integrating sphere is provided with at least four light inlet holes. Inside the semi-integrating sphere, each light inlet hole is correspondingly provided with a vertical circular shading screen; the optical fiber cold light source includes a light source housing, a light source plano-convex lens is arranged inside the light source housing, and a light source housing is provided with a The optical fiber connection port, the incident end of the optical fiber is fixedly connected with the optical fiber connection port, and the outgoing end of the optical fiber is fixedly connected with the light inlet hole respectively, and both extend into the inner cavity of the semi-integrating sphere and face the circular shading screen. The device of the present invention is used to provide a uniform illumination environment for tiny objects, and meets the needs of digital imaging photographing, photographing or spectral imaging observation of a stereo microscope.
Description
Technical Field
The invention relates to the technical field of laboratory lighting devices, in particular to a uniform lighting device for observing tiny objects and a using method thereof.
Background
In order to make the tiny objects clearly imaged in the observation, imaging and measurement instrument or device by a stereoscopic microscope or the like in the reflective observation mode, an external light source is usually required to provide illumination conditions with certain brightness.
Taking a stereomicroscope as an example, a conventional stereomicroscope generally adopts an annular light source to provide annular illumination or a point light source device with double light beams to provide 45-degree illumination, and when the surface of a sample is uneven, the observation result has a shadow or highlight phenomenon, which has a certain influence on microscopic imaging.
The existing integrating sphere technology can provide high-uniformity illumination conditions, but the integrating sphere is usually too large in volume, and the working distance of a microscope is small, so that the integrating sphere is difficult to effectively apply to the microscopic imaging of a stereomicroscope. The prior art records a dome uniform light source device for a microscope, the technology realizes the automatic height adaptation of a half integrating sphere on different multiples, but the quantity and the arrangement position of lamp sets have great influence on the illumination uniformity; in addition, the technique employs LED light sources, the size and spectral characteristics of which limit the application of microscopes in spectral imaging.
Therefore, a device for providing a uniform illumination environment is designed to meet the requirements of digital imaging photographing, camera shooting or spectral imaging observation of a stereoscopic microscope.
Disclosure of Invention
The invention aims to provide a uniform illumination device for observing a tiny object and a using method thereof, which are used for providing relatively uniform illumination conditions when the tiny object is observed and shot through a stereoscopic microscope.
In order to solve the technical problem, the invention provides a uniform illumination device for observing a tiny object, which is arranged below an objective lens of a stereoscopic microscope and comprises a semi-integrating sphere and an optical fiber cold light source;
the semi-integrating sphere is a hollow semi-sphere, a semi-integrating sphere bottom plate is arranged at the bottom of the semi-integrating sphere, the semi-integrating sphere and the semi-integrating sphere bottom plate are enclosed to form a closed space, an observation hole is formed in the center of the semi-integrating sphere bottom plate, a receiving hole is formed in the top of the semi-integrating sphere, and the geometric centers of the receiving hole and the observation hole are on the same straight line; at least four light inlets are arranged on the lateral shell wall of the semi-integrating sphere, and the light inlets are positioned on the same horizontal plane and have equal distance with each other; in the inner cavity of the semi-integrating sphere, each light inlet is correspondingly provided with a vertical circular shading screen;
the optical fiber cold light source comprises a closed light source shell, a light source and a plano-convex lens are arranged in the light source shell, the light source is positioned at the focus position of the plano-convex lens, an optical fiber connecting port is arranged on the light source shell, and the centers of the optical fiber connecting port, the light source and the plano-convex lens are on the same straight line; the incident end of each optical fiber is fixedly connected with the optical fiber connector, the emergent end of each optical fiber is fixedly connected with the light inlet hole, the emergent end of each optical fiber extends into the inner cavity of the half integrating sphere, and the emitted light is over against the circular shading screen.
The invention relates to an improvement of a uniform lighting device for observing tiny objects, which comprises the following steps:
the semi-integrating sphere, the circular shading screen and the semi-integrating sphere bottom plate are made of aluminum alloy, the surfaces of the semi-integrating sphere, the circular shading screen and the semi-integrating sphere bottom plate are all coated with polytetrafluoroethylene coating, and the reflectance ratio is 98%.
The invention relates to a uniform lighting device for observing tiny objects, which is further improved as follows:
the semi-integrating sphere bottom plate and the semi-integrating sphere are fixedly connected into a whole and are placed on the objective table;
the light source and the plano-convex lens are fixedly connected with the light source shell through the metal bracket.
The invention relates to a uniform lighting device for observing tiny objects, which is further improved as follows:
the diameter of the observation hole is 8.0 mm;
the diameter of the receiving hole is 30mm, and an objective lens of the stereoscopic microscope is arranged above the receiving hole;
the diameter of the light inlet hole is 2.0 mm.
The invention relates to a uniform lighting device for observing tiny objects, which is further improved as follows:
the light source is one of a halogen lamp, a xenon lamp or an LED lamp, and preferably, the light source is a halogen lamp.
The invention also provides a using method of the uniform illuminating device for observing the tiny objects, which comprises the following steps:
placing a micro object to be detected on an objective table, and then placing a half integrating sphere on the objective table to enable the micro object to be detected to be located at the center of an observation hole; turning on a light source to provide stable diffuse reflection illumination conditions for the micro object to be detected; then the focal length is adjusted by adjusting the height of the objective lens of the stereomicroscope relative to the receiving hole, and the micro object to be measured is imaged or observed microscopically.
The invention has the following beneficial effects:
1. the invention guides light through the optical fiber, is not influenced by the size and the type of a light source, and then avoids the influence of the heating of the light source on the measurement of a sample;
2. the uniform illumination device provided by the invention has the advantages that through the design of the half integrating sphere, emitted light irradiates an observation area after being subjected to multiple diffuse reflections, and meanwhile, the opening area of the surface of the half integrating sphere is reduced by combining an optical fiber light guiding mode, so that the illumination uniformity of the observation area of the stereoscopic microscope is improved, and the uniform illumination device is particularly suitable for observing tiny objects with higher illumination uniformity;
3. the invention places the micro object to be measured in the closed uniform illumination environment of the semi-integrating sphere, thereby reducing the influence of external environment light on the microscopic imaging;
4. the uniform illumination device is separated from the microscopic digital imaging device and the stereomicroscope, can be used for providing uniform illumination for the imaging of tiny objects, and can also realize the experiment of microscopic observation.
Drawings
The following describes embodiments of the present invention in further detail with reference to the accompanying drawings.
FIG. 1 is a schematic structural diagram of a uniform illumination device for observing tiny objects and a method for using the same according to the present invention;
FIG. 2 is a schematic top view of the half integrating sphere of FIG. 1;
FIG. 3 is a schematic diagram illustrating division of the calculation of the regional illumination uniformity of the receiving surface in the simulation of the light ray tracing method based on Monte Carlo in experiment 1;
fig. 4 is a diagram showing simulation results of uniform illumination provided in experiment 1.
Detailed Description
The invention will be further described with reference to specific examples, but the scope of the invention is not limited thereto:
The semi-integrating sphere 1 is a hollow semi-sphere, the inner diameter is 60.0mm, the outer diameter is 85.0mm, the bottom of the semi-integrating sphere 1 is provided with a semi-integrating sphere bottom plate 5 which is consistent with the bottom shape and size of the semi-integrating sphere 1, the inner wall of the semi-integrating sphere 1 and the semi-integrating sphere bottom plate 5 are made of aluminum alloy, the surfaces of the semi-integrating sphere 1 and the semi-integrating sphere bottom plate 5 are both lambert diffuse reflection surfaces coated with polytetrafluoroethylene coating with high reflectance ratio, and the reflectance ratio is 98%, so that the semi-integrating sphere 1 and the semi-integrating sphere bottom plate 5 enclose a closed space and form a good light reflection effect, and the observed tiny object is uniformly illuminated; a circular observation hole 6 is formed in the center of the bottom plate 5 of the semi-integrating sphere, and the diameter of the observation hole 6 is 8.0 mm; the semi-integrating sphere bottom plate 5 and the semi-integrating sphere 1 are fixedly connected into a whole and are placed on the objective table 7, when the micro-integrating sphere device is used, a micro object to be measured is placed on the objective table 7, then the semi-integrating sphere 1 is placed on the objective table 7, and the micro object to be measured is positioned in the center of the observation hole 6;
the top of the semi-integrating sphere 1 is provided with a circular through hole which is a receiving hole 3, the diameter of the receiving hole 3 is 30mm, the diameter of the receiving hole 3 is slightly larger than that of a microscope objective lens, the circle centers of the receiving hole 3 and an observation hole 6 are on the same straight line, the objective lens of a stereoscopic microscope is arranged above the receiving hole 3, an electronic eyepiece is arranged above the microscope and is used for being connected with a microscopic digital imaging device, the height of the objective lens of the stereoscopic microscope relative to the receiving hole 3 (the height which is lower than the height of the receiving hole 3 enters the interior of the semi-integrating sphere 1 or is higher than the height of the receiving hole 3) is adjusted through a height lifting knob on a stereoscopic microscope arm, and therefore a micro object located at the center of the observation hole 6 can be observed or shot smoothly through focal length adjustment;
four light inlets 4 are arranged on the lateral shell wall of the semi-integrating sphere 1, the diameters of the light inlets 4 are 2.0mm, and the light inlets are equidistant and equal in height, namely the four light inlets 4 are positioned on the same horizontal plane and are equidistant; each light inlet hole 4 is provided with 2M 4 screw bottom holes which are symmetrical left and right;
four circular shading screens 2 are vertically arranged in an inner cavity of the semi-integrating sphere 1, each circular shading screen 2 is positioned at the inner side of a corresponding light inlet 4 and is opposite to the light inlet 4, namely the circle center of the circular shading screen 2 and the circle center of the light inlet 4 are positioned on the same horizontal plane, and the projection of the circular shading screen 2 on the horizontal plane is positioned between the projections of the light inlet 4 and the observation hole 6 on the horizontal plane, so that incident light is ensured not to be directly irradiated on the surface of an object, and the incident light of the light inlet 4 is prevented from being directly emitted or irradiated from a receiving hole 3 to the surface of a tiny object of the observation hole 6; the circular shading screen 2 is made of aluminum alloy, and the surface of the circular shading screen is a Lambert diffuse reflection surface coated with polytetrafluoroethylene coating with high reflectance.
The optical fiber cold light source comprises a closed light source shell 24, a light source 21 and a plano-convex lens 22 are arranged in the light source shell 24, the light source 21 is positioned at the focal point of the plano-convex lens 22, the light source 21 and the plano-convex lens 22 are fixedly connected with the light source shell 24 through a metal support, an optical fiber connecting port 25 is arranged on the light source shell 24, and the centers of the optical fiber connecting port 25, the light source 21 and the plano-convex lens 22 are on the same straight line; the incident end of the optical fiber 23 is fixedly connected with the optical fiber connector 25, the emergent end of the optical fiber 23 is divided into four parts, the four parts are respectively and fixedly connected with the four light inlet holes 4 on the main body of the half integrating sphere 1 through M4 screws, the emergent end of the optical fiber 23 enters the inner cavity of the half integrating sphere 1, and the emergent light is just opposite to the circular shading screen 2, so that diffuse reflection occurs.
The optical fiber 23 is made of glass material and has the characteristics of high heat resistance and high light transmittance; in the example, a 150W halogen lamp is adopted as the light source 21, the emitted light of the light source 21 penetrates through the plano-convex lens 22 to form parallel light, then the parallel light reaches 4 emergent ends of the optical fiber 23 through an incident end of the optical fiber 23, so as to enter the inside of the half integrating sphere 1, and then is shielded by the circular shading screen 2, and the incident light is subjected to multiple diffuse reflection in the inside of the half integrating sphere 1, so as to provide uniform illumination for tiny objects in the observation hole 6; the even illumination device through this embodiment can eliminate the obvious shadow influence that current illumination environment exists, adopts the optic fibre leaded light in this example, avoids light source light stray, the light source generates heat, the light source size is too big, the light source quantity is too much leads to the problem such as structure complicacy, provides stable illumination condition for stereomicroscope observation, micro digital imaging or spectral imaging application.
The using method of the device comprises the following steps:
1. placing a micro object to be detected on an objective table 7, then placing the half integrating sphere 1 on the objective table 7 and enabling the micro object to be detected to be located at the center of the observation hole 6;
2. turning on the light source 21 to provide stable diffuse reflection illumination conditions for the tiny objects to be measured;
3. according to the experimental requirement, a microscopic digital imaging device or a stereoscopic microscope is selected, and the height of an objective lens of the stereoscopic microscope relative to the receiving hole 3 (the height lower than that of the receiving hole 3 enters the inside of the semi-integrating sphere 1 or is higher than that of the receiving hole 3) is adjusted through a height lifting knob on a mirror arm of the stereoscopic microscope, so that the micro object positioned at the center of the observation hole 6 can be smoothly observed or shot through focal length adjustment, and imaging or microscopic observation is carried out.
Experiment 1:
the invention adopts a light ray tracing method based on Monte Carlo to carry out light ray simulation and uniformity analysis on the uniform illumination device for observing the tiny objects and the using method thereof in the embodiment 1. The inner wall of the half integrating sphere 1 and the half integrating sphere bottom plate 5 were set to be lambertian reflecting surfaces, and the reflectance was 0.985. The light source parameters are lattice point light sources, the lattice point graph is circular, the dominant wavelength is 0.54 mu m, and the light beam density is uniformly distributed. The threshold value of the ray tracing is 0.0005, and the ray energy is considered to be absorbed by the inner wall of the half integrating sphere 1 when the energy is reduced to be lower than the threshold value after multiple reflections.
The evaluation method adopts American national standard ANSI/NAMIT 7.228-1997, sets 75% of receiving surface area of the observation hole 6 as receiving surface for illumination uniformity evaluation, sets surface characteristics of the receiving surface as 100% complete absorption, divides the receiving surface into 9 rectangular areas with equal area, and takes the center point of each area as illumination value sampling point and numbers P0-P8 as shown in FIG. 3; four corners of the illumination surface are respectively provided with a sampling point P9-P12, the numbers are respectively positioned on the connecting line of each right-angle vertex and the central point 0P, and the distance between each right-angle vertex and each right-angle vertex is 10% of the distance between the right-angle vertex and the central point 0P; taking the illuminance values of P0-P12 as E0-E12, and Ea as the average value of E0-E8, namely
The uniformity of the illumination surface is defined as N%, and the calculation formula is as follows:
wherein the larger the value of N, the more uniform the illumination.
The uniform illumination device provided by the invention has the advantages that through the design of the half integrating sphere, emitted light irradiates an observation area after being subjected to multiple diffuse reflections, meanwhile, the area of an opening on the surface of the half integrating sphere is reduced by combining an optical fiber light guiding mode, the illumination uniformity of the observation area of the stereoscopic microscope is improved, and the uniform illumination device is particularly suitable for observing a tiny object with higher illumination uniformity. According to the simulation analysis, as shown in fig. 4, the illumination uniformity of the receiving surface area is calculated by simulating the illumination values of the observation area according to the formula (1) and the formula (2), and the simulation calculation result shows that the illumination uniformity at the observation hole 6 of the example 1 of the present invention is 84.72%. Compared with a common annular optical fiber light source device or a double-beam point light source device, the invention improves the higher illumination uniformity of the observation area and avoids the obvious highlight phenomenon caused by the fact that the emitted light of the light source directly irradiates the surface of the observed object.
Finally, it should be noted that the above lists only a few specific embodiments of the present invention, and for better illumination uniformity, the objective of observation under different magnifications of the microscope can be achieved by optimizing parameters such as the size of the half integrating sphere, the open pore surface area of the half integrating sphere, the number of optical fibers, and the like. It is obvious that the invention is not limited to the above embodiments, but that many variations are possible. All modifications which can be derived or suggested by a person skilled in the art from the disclosure of the present invention are to be considered within the scope of the invention.
Claims (6)
1. The uniform lighting device for observing the tiny objects is arranged below an objective lens of a stereoscopic microscope, and is characterized in that: comprises a semi-integrating sphere (1) and an optical fiber cold light source;
the semi-integrating sphere (1) is a hollow semi-sphere, a semi-integrating sphere bottom plate (5) is arranged at the bottom of the semi-integrating sphere, the semi-integrating sphere (1) and the semi-integrating sphere bottom plate (5) are enclosed to form a closed space, an observation hole (6) is formed in the center of the semi-integrating sphere bottom plate (5), a receiving hole (3) is formed in the top of the semi-integrating sphere (1), and the geometric centers of the receiving hole (3) and the observation hole (6) are on the same straight line; at least four light inlets (4) are arranged on the lateral shell wall of the semi-integrating sphere (1), and the light inlets (4) are positioned on the same horizontal plane and have equal distance; in the inner cavity of the semi-integrating sphere (1), each light inlet hole (4) is correspondingly provided with a vertical circular shading screen (2);
the optical fiber cold light source comprises a closed light source shell (24), a light source (21) and a planoconvex lens (22) are arranged in the light source shell (24), the light source (21) is positioned at the focus position of the planoconvex lens (22), an optical fiber connecting port (25) is arranged on the light source shell (24), and the centers of the optical fiber connecting port (25), the light source (21) and the planoconvex lens (22) are on the same straight line; the incident end of each optical fiber (23) is fixedly connected with the optical fiber connector (25), the emergent end of each optical fiber (23) is fixedly connected with the corresponding light inlet hole (4), the emergent end of each optical fiber (23) extends into the inner cavity of the corresponding half integrating sphere (1), and the emitted light is opposite to the circular shading screen (2).
2. The uniform illumination device for observing tiny objects as claimed in claim 1, wherein:
the semi-integrating sphere (1), the circular shading screen (2) and the semi-integrating sphere bottom plate (5) are made of aluminum alloy, polytetrafluoroethylene coating is coated on the surfaces of the semi-integrating sphere bottom plate and the semi-integrating sphere bottom plate, and the reflectance ratio is 98%.
3. The uniform illumination device for observing tiny objects as claimed in claim 2, wherein:
the semi-integrating sphere bottom plate (5) and the semi-integrating sphere (1) are fixedly connected into a whole and are placed on the objective table (7);
the light source (21) and the plano-convex lens (22) are fixedly connected with the light source shell (24) through a metal bracket.
4. The uniform illumination device for observing tiny objects as claimed in claim 3, wherein:
the diameter of the observation hole (6) is 8.0 mm;
the diameter of the receiving hole (3) is 30mm, and the objective lens of the stereomicroscope is arranged above the receiving hole (3);
the diameter of the light inlet hole (4) is 2.0 mm.
5. The uniform illumination device for observing tiny objects as claimed in claim 4, wherein:
the light source (21) is a halogen lamp, a xenon lamp or an LED lamp.
6. The use method of the uniform illumination device for observing the tiny objects as claimed in any one of claims 1 to 5, wherein:
placing a micro object to be detected on an objective table (7), then placing a semi-integrating sphere (1) on the objective table (7) and enabling the micro object to be detected to be located at the center of an observation hole (6); turning on a light source (21) to provide stable diffuse reflection illumination conditions for the tiny objects to be detected; then, the focal length is adjusted by adjusting the height of the objective lens of the stereomicroscope relative to the receiving hole (3), and the micro object to be measured is imaged or microscopically observed.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202111028904.1A CN113687504A (en) | 2021-09-03 | 2021-09-03 | Uniform lighting device for observing tiny objects and using method thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202111028904.1A CN113687504A (en) | 2021-09-03 | 2021-09-03 | Uniform lighting device for observing tiny objects and using method thereof |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN113687504A true CN113687504A (en) | 2021-11-23 |
Family
ID=78585088
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202111028904.1A Pending CN113687504A (en) | 2021-09-03 | 2021-09-03 | Uniform lighting device for observing tiny objects and using method thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN113687504A (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2024025930A3 (en) * | 2022-07-27 | 2024-03-28 | Trustees Of Boston University | Efficient and uniform color-light integration device |
| CN118168481A (en) * | 2024-05-14 | 2024-06-11 | 首都体育学院 | Combined light source for detecting surface roughness of equipment |
Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN200965487Y (en) * | 2006-07-07 | 2007-10-24 | 浙江大学三色仪器有限公司 | A flat display bright-room contrast ratio measurement device |
| JP2012227201A (en) * | 2011-04-15 | 2012-11-15 | Citizen Holdings Co Ltd | Inspection device and inspection method for semiconductor light-emitting device |
| CN103575508A (en) * | 2012-07-19 | 2014-02-12 | 大塚电子株式会社 | Optical characteristic measuring apparatus |
| CN104034663A (en) * | 2013-03-07 | 2014-09-10 | 中国计量学院 | Material space reflection characteristic measuring instrument using optical fiber bundles |
| CN209117182U (en) * | 2018-11-20 | 2019-07-16 | 杭州远方光电信息股份有限公司 | A kind of color measuring device |
| CN110954301A (en) * | 2018-09-26 | 2020-04-03 | 大塚电子株式会社 | Measurement system and measurement method |
-
2021
- 2021-09-03 CN CN202111028904.1A patent/CN113687504A/en active Pending
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN200965487Y (en) * | 2006-07-07 | 2007-10-24 | 浙江大学三色仪器有限公司 | A flat display bright-room contrast ratio measurement device |
| JP2012227201A (en) * | 2011-04-15 | 2012-11-15 | Citizen Holdings Co Ltd | Inspection device and inspection method for semiconductor light-emitting device |
| CN103575508A (en) * | 2012-07-19 | 2014-02-12 | 大塚电子株式会社 | Optical characteristic measuring apparatus |
| CN104034663A (en) * | 2013-03-07 | 2014-09-10 | 中国计量学院 | Material space reflection characteristic measuring instrument using optical fiber bundles |
| CN110954301A (en) * | 2018-09-26 | 2020-04-03 | 大塚电子株式会社 | Measurement system and measurement method |
| CN209117182U (en) * | 2018-11-20 | 2019-07-16 | 杭州远方光电信息股份有限公司 | A kind of color measuring device |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2024025930A3 (en) * | 2022-07-27 | 2024-03-28 | Trustees Of Boston University | Efficient and uniform color-light integration device |
| US12140775B2 (en) | 2022-07-27 | 2024-11-12 | Trustees Of Boston University | Efficient and uniform color-light integration device |
| CN118168481A (en) * | 2024-05-14 | 2024-06-11 | 首都体育学院 | Combined light source for detecting surface roughness of equipment |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| AU2017265107B2 (en) | Artificial lighting system for simulating a natural lighting | |
| CN113687504A (en) | Uniform lighting device for observing tiny objects and using method thereof | |
| Gage | Modern dark-field microscopy and the history of its development | |
| CN206945525U (en) | A kind of spectral transmittance detection instrument | |
| CN204462528U (en) | Lighting device and stereomicroscope | |
| CN111929886A (en) | Dark field illuminator for microscopic imaging | |
| CN111442908B (en) | Device and method for detecting visible light transmittance and uniformity of optical fiber image transmission element | |
| JP6140407B2 (en) | Microscope with transmitted light illuminator for critical illumination | |
| CN204302565U (en) | Compact microscope | |
| JP2008026924A (en) | Stereomicroscope transmission illuminating device | |
| CN111929881A (en) | Phase object imaging device and method based on chromatic dispersion | |
| JP2880358B2 (en) | Lighting equipment | |
| CN213422087U (en) | Multi-view-angle rapid shooting device for cephalopod cutin and jaw | |
| CN105181303B (en) | Infinity conjugate distance microcobjective Stray Light Testing Equipment and measuring accuracy adjusting method | |
| CN208092358U (en) | A kind of multiband suitching type parallel light tube | |
| CN215297818U (en) | Light microscope capable of being used for inspecting microscopic samples | |
| CN111272640A (en) | Illuminating device for sheath flow optical imaging | |
| JPH0423217Y2 (en) | ||
| CN216696835U (en) | Miniature single section of thick bamboo metallography video microscope of modularization | |
| CN218630343U (en) | Special low-light-level visual field instrument for night vision | |
| CN222597486U (en) | A light source that can be used in cell imaging instruments | |
| CN211857047U (en) | Microscope and analyzer | |
| CN203069356U (en) | Fibre faceplate limiting resolution test instrument based on shade elimination | |
| CN115128788B (en) | Place the microscope device horizontally parallel to the observation object | |
| CN110515190B (en) | Portable back-illuminated optical imaging microscope |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| WD01 | Invention patent application deemed withdrawn after publication | ||
| WD01 | Invention patent application deemed withdrawn after publication |
Application publication date: 20211123 |