CN110515190B - Portable back-illuminated optical imaging microscope - Google Patents
Portable back-illuminated optical imaging microscope Download PDFInfo
- Publication number
- CN110515190B CN110515190B CN201910898252.3A CN201910898252A CN110515190B CN 110515190 B CN110515190 B CN 110515190B CN 201910898252 A CN201910898252 A CN 201910898252A CN 110515190 B CN110515190 B CN 110515190B
- Authority
- CN
- China
- Prior art keywords
- objective lens
- light
- axis direction
- imaging
- optical imaging
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
- 238000012634 optical imaging Methods 0.000 title claims abstract description 35
- 238000003384 imaging method Methods 0.000 claims abstract description 67
- 238000005286 illumination Methods 0.000 claims abstract description 56
- 238000006073 displacement reaction Methods 0.000 claims abstract description 32
- 238000004891 communication Methods 0.000 claims description 11
- 230000003287 optical effect Effects 0.000 claims description 8
- 238000000034 method Methods 0.000 claims description 5
- 241000276498 Pollachius virens Species 0.000 abstract description 11
- 238000013461 design Methods 0.000 abstract description 4
- 230000005540 biological transmission Effects 0.000 description 6
- 229910052736 halogen Inorganic materials 0.000 description 4
- 150000002367 halogens Chemical class 0.000 description 4
- 230000035939 shock Effects 0.000 description 3
- 230000000694 effects Effects 0.000 description 2
- WFKWXMTUELFFGS-UHFFFAOYSA-N tungsten Chemical compound [W] WFKWXMTUELFFGS-UHFFFAOYSA-N 0.000 description 2
- 229910052721 tungsten Inorganic materials 0.000 description 2
- 239000010937 tungsten Substances 0.000 description 2
- 230000007547 defect Effects 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 238000012632 fluorescent imaging Methods 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 239000000178 monomer Substances 0.000 description 1
- 238000012545 processing Methods 0.000 description 1
- 238000012546 transfer Methods 0.000 description 1
Classifications
-
- G—PHYSICS
- G02—OPTICS
- G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
- G02B21/00—Microscopes
- G02B21/06—Means for illuminating specimens
-
- G—PHYSICS
- G02—OPTICS
- G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
- G02B21/00—Microscopes
- G02B21/36—Microscopes arranged for photographic purposes or projection purposes or digital imaging or video purposes including associated control and data processing arrangements
- G02B21/361—Optical details, e.g. image relay to the camera or image sensor
Landscapes
- Physics & Mathematics (AREA)
- Chemical & Material Sciences (AREA)
- Analytical Chemistry (AREA)
- General Physics & Mathematics (AREA)
- Optics & Photonics (AREA)
- Engineering & Computer Science (AREA)
- Multimedia (AREA)
- Microscoopes, Condenser (AREA)
Abstract
The invention relates to the technical field of microscopes and discloses a portable back-illuminated optical imaging microscope which comprises an objective lens, a Z-direction displacement control module, an XY-direction displacement control module, a back-illuminated module, an imaging unit switching module, an imaging unit and an eyepiece. Therefore, the distance which is required to be introduced by adopting the Kohler illumination light path structure is shortened, and the structure and the volume of the microscope are further reduced. Therefore, the design adopts the plane LED light source as the lighting system, thereby greatly reducing the cost and the occupied space of the whole system.
Description
Technical Field
The invention relates to the technical field of microscopes, in particular to a portable back-illuminated optical imaging microscope.
Background
The general optical imaging microscope refers to an optical imaging microscope using conventional visible light for illumination, and the light source is different from a fluorescent imaging microscope and a nonlinear imaging microscope. The imaging illumination mode and the application of the common optical imaging microscope are introduced as follows:
the illumination modes mainly include a forward transmission type illumination (the illumination light penetrates through the sample and enters the objective lens) and a backward illumination reflection type (the illumination light irradiates the sample through the objective lens and returns to the objective lens after being reflected by the sample) optical microscope.
The application and the corresponding technical structure and process thereof:
Both illumination techniques are based on the principle of kohler illumination, which is the illumination of a sample using an image of a filament as a secondary light source. The purpose is to eliminate the uneven illumination caused by illumination by direct imaging of the filament on the sample. The basic principle of secondary illumination is that the wavelet superposition principle is adopted, namely, each point on the filament image surface is used as a point light source to carry out secondary light emission, illumination is carried out according to the wavelet superposition principle, and the advantage of the fact that illumination at a sample is basically uniform illumination is achieved.
The forward transmission type illumination microscope is mainly a biological microscope, and the backward illumination reflection type microscope is mainly a metallographic microscope.
The two illumination modes have the problems and the disadvantages that:
The microscope adopting the Kohler illumination mode has relatively large volume, relatively high price and is unfavorable for portable monomer operation. Although there are also transmission optical microscopes that use LEDs as light sources at low cost, they are somewhat bulky, inconvenient to carry, and susceptible to sample transparency. That is, existing cheaper optical imaging microscopes typically employ forward transmission illumination to produce imaging specimens using transparent sample preparation. Such an imaging microscope is already untimely for special opaque samples.
The lens barrel length and the displacement mode of adjusting the sample lead to the fact that the microscope is not portable and is very heavy, and meanwhile, the magnification of the microscope is limited due to the fact that the magnification of the used objective lens and eyepiece is smaller. The optical imaging microscope is an imaging organism composed of an objective lens and an illumination light source, so that an optical imaging rule is necessarily satisfied. While the traditional biological optical imaging microscope mainly adopts transmission type illumination, which leads to the observation of a sample to be basically transparent, the metallographic microscope adopts a back illumination imaging mode, but adopts a halogen lamp or a halogen tungsten lamp as an illumination light source, and the illumination mode is influenced by the traditional kohler illumination imaging optical principle (the image of an illumination light source filament is taken as a light source to illuminate the sample), so that the imaging microscope has larger size and higher price.
Disclosure of Invention
The invention aims to provide a portable back-illuminated optical imaging microscope, which aims to solve the problems of single function and inconvenient carrying of the microscope in the prior art
The portable back illumination optical imaging microscope comprises an objective lens, a Z-direction displacement control module, an XY-direction displacement control module, a back illumination module, an imaging unit switching module, an imaging unit and an eyepiece, wherein the Z-direction displacement control module is used for controlling the distance between the objective lens and a sample to be observed, the XY-direction displacement control module is used for controlling the position of an observation field of the objective lens, the back illumination module comprises a half-plane LED illumination light source, a beam splitting flat plate, a mounting fixing piece used for mounting the half-plane LED illumination light source and the beam splitting flat plate and a displacement groove, the mounting fixing piece is slidably arranged in the displacement groove, the imaging unit is used for receiving light signals and generating image data according to the received light signals, the imaging unit switching module comprises a slidable reflecting mirror, light emitted by the half-plane LED light source enters the objective lens through reflection of the beam splitting flat plate, the sample to be observed is irradiated, the sample to be observed reflects the light back to the objective lens and then transmits the beam splitting flat plate to form first light, and when the first light slides to the first reflecting mirror, and the imaging unit is used for imaging the first light to the other person to the position when the first light is reflected to the first reflecting mirror.
Further, the imaging unit transfer module further comprises an adapter, the adapter comprises a first light ray channel which is horizontally arranged and a second light ray channel which is vertically arranged, the middle of the first light ray channel is communicated with the middle of the second light ray channel, the first light ray channel is oppositely arranged with the ocular, the second light ray channel is oppositely arranged with the imaging unit, the adapter is provided with a sliding groove, and the reflecting mirror is slidably arranged in the sliding groove.
Further, the XY direction displacement control module includes an X-axis direction screw arranged in an X-axis direction, the X-axis direction screw being for abutting against the objective lens to effect X-direction displacement of the objective lens, and a Y-axis direction screw arranged in a Y-axis direction, the Y-axis direction screw being for abutting against the objective lens to effect Y-direction displacement of the objective lens.
Further, the Z-direction displacement control module comprises a screw rod arranged along the Z-axis direction, the screw rod is connected with the objective lens, and when the screw rod is rotated, the objective lens moves along the Z-axis direction.
The imaging unit comprises an imaging sensor, an AD converter, a singlechip and a display, wherein the imaging sensor is used for converting an optical signal into an electric signal and transmitting the electric signal to the AD converter, the AD converter is used for processing the electric signal sent by the imaging sensor, converting the electric signal into a digital signal and then transmitting the digital signal to the singlechip, and the singlechip is used for encoding the digital signal sent by the AD converter to obtain image data.
Further, the imaging sensor is a CCD sensor.
Further, the imaging sensor is a CMOS sensor.
Further, the portable back-illuminated optical imaging microscope comprises a communication module for establishing communication connection with the terminal, and the communication module is connected with the singlechip.
Further, a shock pad is arranged on the periphery of the objective lens in a surrounding mode.
Compared with the prior art, the portable back-illuminated optical imaging microscope provided by the invention avoids the traditional kohler illumination light source structure, and the light source does not need to illuminate a sample by taking a secondary imaged image as the light source, but directly adopts an LED with the surface light source characteristic as the light source to illuminate in a direct back-illuminated mode. Therefore, the distance which is required to be introduced by adopting the Kohler illumination light path structure is shortened, so that the structure of the microscope is further reduced. Therefore, the design adopts the plane LED light source as the lighting system, thereby greatly reducing the cost and the occupied space of the whole system.
Drawings
FIG. 1 is a schematic perspective view of a portable back-illuminated optical imaging microscope provided in an embodiment of the present invention;
FIG. 2 is an exploded view of a portable back-illuminated optical imaging microscope provided in accordance with an embodiment of the present invention;
FIG. 3 is an exploded schematic view of an imaging unit adapter module and an imaging unit provided by an embodiment of the present invention;
Fig. 4 is an exploded view of a back-lighting module according to an embodiment of the present invention.
Detailed Description
The present invention will be described in further detail with reference to the drawings and examples, in order to make the objects, technical solutions and advantages of the present invention more apparent. It should be understood that the specific embodiments described herein are for purposes of illustration only and are not intended to limit the scope of the invention.
In the description of the present invention, it should be understood that, if there is an orientation or positional relationship indicated by terms such as "upper", "lower", "left", "right", etc. based on the orientation or positional relationship shown in the drawings, it is merely for convenience of describing the present invention and simplifying the description, and it is not intended to indicate or imply that the apparatus or elements referred to must have a specific orientation, be constructed and operated in a specific orientation, and thus the terms describing the positional relationship in the drawings are merely for exemplary illustration and are not to be construed as limitations of the present patent, and that the specific meaning of the terms described above may be understood by those skilled in the art according to specific circumstances.
The implementation of the present invention will be described in detail below with reference to specific embodiments.
Referring to fig. 1-4, a preferred embodiment of the present invention is provided.
The portable back illumination optical imaging microscope comprises an objective lens 1, a Z-direction displacement control module, an XY-direction displacement control module 2, a back illumination module 5, an imaging unit switching module 6, an imaging unit 3 and an eyepiece 4;Z, wherein the XY-direction displacement control module 2 is used for controlling the distance between the objective lens 1 and a sample to be observed, the XY-direction displacement control module 2 is used for controlling the position of an observation field of the objective lens 1, the back illumination module 5 comprises a half-plane LED illumination light source 51, a light splitting flat plate 52, a mounting fixing piece 53 for mounting the half-plane LED illumination light source 51 and the light splitting flat plate 52 and a displacement groove 54, the mounting fixing piece 53 is slidably arranged in the displacement groove 54, the imaging unit 3 is used for receiving light signals and generating image data according to the received light signals, the imaging unit switching module 6 comprises a slidable reflector 62, light emitted by the half-plane LED light source enters the objective lens 1 through reflection of the light splitting flat plate 52, the sample to be observed is irradiated, the sample to be observed is reflected back to the objective lens 1, the light splitting flat plate 52 forms first light rays, when the first light rays slide to the opposite to the first light rays, the first light rays are reflected to the reflector 62 through the reflector 3 and then slide to the first light rays for the first eye lens 4 to be directly observed.
The imaging principle of the portable back-illuminated optical imaging microscope provided by the above-mentioned method is that the light emitted by the half-plane LED light source enters the objective lens 1 through the reflection of the light splitting flat plate 52, irradiates the sample to be observed, the sample to be observed reflects the light back to the objective lens 1, then transmits the light splitting flat plate 52 to form first light, when the reflector 62 slides to the opposite position of the first light, the first light is reflected to the imaging unit 3 through the reflector 62 for imaging, and when the reflector 62 slides to other positions, the first light directly enters the ocular lens 4 for human eye observation.
The traditional biological optical imaging microscope mainly adopts transmission type illumination, so that the observed sample is basically transparent slice, and the metallographic microscope adopts a back illumination imaging mode, but adopts a halogen lamp or a halogen tungsten lamp as an illumination light source, and the illumination mode is influenced by the traditional kohler illumination imaging optical principle (the image of an illumination light source filament is taken as a light source to illuminate the sample). Resulting in a larger size and higher price of the imaging microscope.
The invention discloses a portable back-illuminated optical imaging microscope aiming at the defects of the traditional microscope caused by the adoption of a Kohler illumination structure. The inventive design is performed in order to solve the contradiction between displacement and portable size and to observe imaging stability.
The portable back-illuminated optical imaging microscope provided by the invention avoids the traditional Kohler illumination light source structure, the light source does not need to illuminate a sample by taking a secondary imaged image as the light source, and an LED with a surface light source characteristic is directly selected as the light source for direct back illumination. Therefore, the distance which is required to be introduced by adopting the Kohler illumination light path structure is shortened, so that the structure of the microscope is further reduced. Therefore, the design adopts the plane LED light source as the lighting system, thereby greatly reducing the cost and the occupied space of the whole system.
The imaging principle of the imaging system is explained in that when a uniform light source acts on an object, secondary diffused light on the surface of the object is imaged through a lens group, and the imaging principle also meets the geometric optical Gaussian imaging principle.
The core technology of the invention is mainly characterized in that the illumination mode breaks through the limitation of the traditional optical imaging microscope by the light source of the Kohler illumination imaging system. The cheaper surface element type white light LED is used as a light source, so that the core part is cheaper, and the convenience of the portable microscope in field operation is improved.
Specifically, the imaging unit adapting module 6 further includes an adapter 61, the adapter 61 includes a first light path 612 disposed horizontally and a second light path 613 disposed vertically, the middle of the first light path 612 is communicated with the middle of the second light path 613, the first light path 612 is disposed opposite to the eyepiece 4, the second light path 613 is disposed opposite to the imaging unit 3, the adapter 61 has a chute 611, and the mirror 62 is slidably disposed in the chute 611, such that when the mirror 62 is slid to the opposite of the first light, the first light is reflected by the mirror 62 to the imaging unit 3 for imaging, and when the mirror 62 is slid to other positions, the first light directly enters the eyepiece 4 for viewing by a human eye.
Specifically, the XY direction displacement control module 2 includes an X-axis direction screw 21 and a Y-axis direction screw 22, the X-axis direction screw 21 is arranged in the X-axis direction, the X-axis direction screw 21 is used for abutting against the objective lens 1, the Y-axis direction screw 22 is arranged in the Y-axis direction, and the Y-axis direction screw 22 is used for abutting against the objective lens 1, so that by screwing the X-axis direction screw 21, the movement of the objective lens 1 in the X-axis direction can be controlled, by screwing the Y-axis direction screw 22, the movement of the objective lens 1 in the X-axis direction and the Y-axis direction can be realized, and further, the control of the position of the observation field of the objective lens 1 can be realized.
And the Z-direction displacement control module comprises a screw rod which is arranged along the Z-axis direction and is in threaded connection with the objective lens 1, and when the screw rod is rotated, the objective lens 1 moves along the Z-axis direction, so that the distance between the objective lens 1 and a sample to be observed is controlled.
In this embodiment, the imaging unit 3 includes an imaging sensor, an AD converter, a single-chip microcomputer, and a display, where the imaging sensor is configured to convert an optical signal into an electrical signal and transmit the electrical signal to the AD converter, the AD converter is configured to process the electrical signal sent by the imaging sensor, convert the electrical signal into a digital signal, and send the digital signal to the single-chip microcomputer, and the single-chip microcomputer is configured to encode the digital signal sent by the AD converter to obtain image data.
In this embodiment, the imaging sensor is a CCD sensor.
Or as other embodiments, the imaging sensor is a CMOS sensor.
In the embodiment, the portable back-illuminated optical imaging microscope comprises a communication module for establishing communication connection with the terminal, wherein the communication module is connected with the single chip microcomputer, so that the communication connection is established between the communication module and the terminal, the image data obtained by encoding the single chip microcomputer is transmitted to the terminal, and the terminal stores and displays the image data.
Further, the periphery of the objective lens 1 is annularly provided with a shock pad, and the arrangement of the shock pad breaks through the limitation of practical environment, so that the portable back-illuminated optical imaging microscope can be suitable for wider use scenes and environments.
The foregoing description of the preferred embodiments of the invention is not intended to be limiting, but rather is intended to cover all modifications, equivalents, and alternatives falling within the spirit and principles of the invention.
Claims (9)
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910898252.3A CN110515190B (en) | 2019-09-23 | 2019-09-23 | Portable back-illuminated optical imaging microscope |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910898252.3A CN110515190B (en) | 2019-09-23 | 2019-09-23 | Portable back-illuminated optical imaging microscope |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN110515190A CN110515190A (en) | 2019-11-29 |
| CN110515190B true CN110515190B (en) | 2025-01-28 |
Family
ID=68633215
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201910898252.3A Active CN110515190B (en) | 2019-09-23 | 2019-09-23 | Portable back-illuminated optical imaging microscope |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN110515190B (en) |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN210534427U (en) * | 2019-09-23 | 2020-05-15 | 深圳市笨辉光电科技有限公司 | Portable back-lighting optical imaging microscope |
Family Cites Families (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP3647062B2 (en) * | 1993-05-17 | 2005-05-11 | オリンパス株式会社 | Upright microscope |
| CN2798122Y (en) * | 2004-11-15 | 2006-07-19 | 宁海洋 | Portable digital microscope |
| JP5962968B2 (en) * | 2012-04-13 | 2016-08-03 | 株式会社ニコン | Microscope adjustment method and microscope |
| DE102012214568A1 (en) * | 2012-08-16 | 2014-02-20 | Leica Microsystems Cms Gmbh | Optical arrangement and a microscope |
| DE102013213781A1 (en) * | 2013-03-20 | 2014-09-25 | Leica Microsystems Cms Gmbh | Method and optical arrangement for manipulating and imaging a microscopic sample |
| CN104390943B (en) * | 2014-11-24 | 2017-07-18 | 中国科学院苏州生物医学工程技术研究所 | It is a kind of while obtaining the micro imaging system of appearance images and Elemental redistribution image |
| WO2016174963A1 (en) * | 2015-04-30 | 2016-11-03 | 住友電気工業株式会社 | Microscope device |
| JP6241858B2 (en) * | 2016-06-02 | 2017-12-06 | レーザーテック株式会社 | Confocal microscope |
| JP2019032464A (en) * | 2017-08-09 | 2019-02-28 | 国立大学法人電気通信大学 | Portable filterless microscope and microscopic observation method using the same |
| CN109839732B (en) * | 2019-01-30 | 2023-10-20 | 中国科学技术大学 | Cage structure laser scanning confocal microscopic imaging system and method |
| CN109991725B (en) * | 2019-04-11 | 2024-08-09 | 中国科学院脑科学与智能技术卓越创新中心 | Portable miniature fluorescent microscope |
-
2019
- 2019-09-23 CN CN201910898252.3A patent/CN110515190B/en active Active
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN210534427U (en) * | 2019-09-23 | 2020-05-15 | 深圳市笨辉光电科技有限公司 | Portable back-lighting optical imaging microscope |
Also Published As
| Publication number | Publication date |
|---|---|
| CN110515190A (en) | 2019-11-29 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| EP2633365B1 (en) | Light emitting diode projector | |
| US4148552A (en) | Microscope adaptable to various observing methods | |
| US7636204B1 (en) | 360 degree view imaging system | |
| CN210534427U (en) | Portable back-lighting optical imaging microscope | |
| CN111338067A (en) | A miniature fluorescence microscope imaging module | |
| CN107621692B (en) | Objective lens module and microscope | |
| CN110515190B (en) | Portable back-illuminated optical imaging microscope | |
| CN114136937A (en) | Multifunctional miniature fluorescent microscopic imaging device and imaging method thereof | |
| JP2012018313A (en) | Two-dimensional measuring device | |
| CN105116539A (en) | Portable precision digital optical microscope | |
| EP3084501A1 (en) | Light source adaptor for a microscope | |
| WO2021189453A1 (en) | Miniature fluorescence microscopy imaging module | |
| CN216525449U (en) | Multifunctional miniature fluorescent dark field microscopic imaging device | |
| KR20020059126A (en) | A handheld digital microscope, skin management system using this and cf-type ccd camera joint with this | |
| CN211603703U (en) | Small-volume microscopic light source | |
| CN209951230U (en) | Light source device suitable for confocal oral scanner | |
| CN211878296U (en) | Micro-imaging module of miniature fluorescence | |
| CN207502807U (en) | A kind of objective lens module and microscope | |
| US8848031B2 (en) | Digital microscope system | |
| JP3379820B2 (en) | TV camera device for skin diagnosis | |
| CN110426835A (en) | A kind of hand-held continuous zoom microscopie unit | |
| CN219496783U (en) | Miniaturized automatic focusing device | |
| JP3434064B2 (en) | Epi-illumination phase contrast microscope | |
| CN219921015U (en) | Endoscope lens and endoscope system | |
| CN220751948U (en) | Multifunctional microscopic imaging instrument |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |