CN113454229A - 用于治疗高草酸尿症的方法和组合物 - Google Patents
用于治疗高草酸尿症的方法和组合物 Download PDFInfo
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- CN113454229A CN113454229A CN201980090574.3A CN201980090574A CN113454229A CN 113454229 A CN113454229 A CN 113454229A CN 201980090574 A CN201980090574 A CN 201980090574A CN 113454229 A CN113454229 A CN 113454229A
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Abstract
本公开内容总体上涉及已被修饰成具有增加的草酸盐降解活性的细菌，包括所述细菌的药物组合物，以及治疗与草酸盐量升高相关的病症(例如，高草酸尿症)的方法。
Description
相关申请的交叉引用
本申请要求2018年12月10日提交的美国临时专利申请系列号62/777,322的权益和优先权，其通过引用整体并入本文。
背景技术
草酸是最小的二元羧酸，经常作为有用的次级代谢物以及分解代谢过程的副产品出现在自然界中。植物产生并积累大量的草酸盐作为毒害食草动物和防止捕食的一种方式。一些代谢过程也会在哺乳动物体内产生草酸盐。必须处理有毒的草酸盐，且在哺乳动物中，这通过将草酸盐运输到尿和粪便中来完成。但是，如果将过多的草酸盐排入尿，则会发生高草酸尿症。
高草酸尿症是在尿中排出过量草酸盐的病症。升高的尿草酸盐已经与复发性草酸钙肾结石、肾损伤和最终终末期肾病相关。高草酸尿症根据过量草酸盐的主要来源分为四类：原发性、肠源性、饮食性和特发性高草酸尿症。原发性高草酸尿症涉及由遗传原因导致的高草酸尿症；它的三种形式I、II和III分别由人基因AGXT、GRHPR和HOGA1中的突变造成。这些基因中的任一种的突变都会导致肝脏中由内源性过程产生的草酸盐的水平增加。无法确定遗传原因的高草酸尿症的形式(肠源性、饮食性和特发性高草酸尿症)被称为继发性高草酸尿症。虽然从饮食中吸收某些基线水平的草酸盐是正常的，但在患有肠源性高草酸尿症的患者中，肠道对草酸盐的渗透性已经增加，从而导致从饮食中吸收更多的草酸盐。这可以由病症(包括炎性肠病和胃旁路)导致的肠道生理紊乱造成。饮食性高草酸尿症源于饮食中草酸盐或草酸盐前体的过度消耗。最后，对于具有特发性高草酸尿症的患者，其高草酸尿症的急性原因尚未确定。
几乎没有治疗高草酸尿症的方法。对于具有AXGT中的突变的原发性高草酸尿症患者，可以开出吡多辛(pyridoxine)处方以挽救突变酶的某些活性。备选地，原发性高草酸尿症患者可以接受肝脏移植以替代缺失的肝酶。对于其余的高草酸尿症患者，与旨在纠正并发症(肾结石和肾衰竭)的其它治疗方法一起，高钙、低草酸盐饮食是主要干预措施。但是，即使小心饮食也难以避免草酸盐，且指导患者避免草酸盐往往是不够的。由于可用于缓解高草酸尿症的治疗方法有限，因此患者需要一种强效且稳定的治疗方法来从其身体除去草酸盐。
因此，持续需要新的且有效的疗法来治疗和管控与升高的量的草酸盐相关的疾病或病症，例如高草酸尿症。
发明内容
本公开内容总体上涉及已被修饰成具有增加的草酸盐降解活性的细菌。公开的细菌可用于治疗受试者中与草酸盐量升高相关的病症，例如高草酸尿症。
例如，在一个方面，本文提供了一种共生细菌，与未经修饰的类似的或在其它方面相同的细菌相比，其已被修饰成具有增加的草酸盐降解活性。在某些实施方案中，未经修饰的类似的或在其它方面相同的细菌不具有可检测到的草酸盐降解活性和/或不具有已知参与(例如直接参与)草酸盐分解代谢的基因。在某些实施方案中，与细菌相同属的天然存在成员不具有可检测到的草酸盐降解活性。
在某些实施方案中，所涵盖的细菌属于选自以下的属：拟杆菌属(Bacteroides)、另枝菌属(Alistipes)、栖粪杆菌属(Faecalibacterium)、副拟杆菌属(Parabacteroides)、普雷沃氏菌属(Prevotella)、罗氏菌属(Roseburia)、瘤胃球菌属(Ruminococcus)、梭菌属(Clostridium)、颤杆菌属(Oscillibacter)、芽殖菌属(Gemmiger)、巴恩斯氏菌(Barnesiella)、戴阿利斯特杆菌属(Dialister)、副萨特氏菌属(Parasutterella)、考拉杆菌属(Phascolarctobacterium)、丙酸杆菌属(Propionibacterium)、萨特氏菌属(Sutterella)、布劳特氏菌属(Blautia)、副普雷沃氏菌属(Paraprevotella)、粪球菌属(Coprococcus)、臭菌属(Odoribacter)、螺原体属(Spiroplasma)、厌氧棒状菌属(Anaerostipes)和阿克曼氏菌属(Akkermansia)。例如，所涵盖的细菌可以属于拟杆菌属(Bacteroides)，即，可以是拟杆菌属各种的细菌。
在某些实施方案中，所涵盖的细菌包含一种或多种编码蛋白或其功能片段或变体的转基因，所述蛋白选自：草酸盐:甲酸盐反向转运蛋白(OxlT)；草酸脱羧酶(OXDC)-EC4.1.1.2；草酸氧化酶(OXO)-EC 1.2.3.4；草酸氧化还原酶(OOR)-EC 1.2.7.10；草酸辅酶A连接酶/草酰辅酶A合成酶(OXS)-EC 6.2.1.8；甲酰辅酶A:草酸辅酶A转移酶(FCOCT)-EC2.8.3.16；乙酰辅酶A:草酸辅酶A转移酶(ACOCT)-EC 2.8.3.19；琥珀酰辅酶A:草酸辅酶A转移酶(SCOCT)-EC 2.8.3.2；草酰辅酶A脱羧酶(OXC)-EC 4.1.1.8；草酰辅酶A还原酶/乙醛酸盐:NADP+氧化还原酶(OXR)-EC 1.2.1.17；和前述蛋白任何的组合。预期一种或多种转基因可以，例如，在质粒、细菌人工染色体上，或整合在基因组中。当细菌包含一种或多种编码多种蛋白的转基因时，预期编码两种或更多种蛋白的开放读码框可以例如存在于单个操纵子中。
在某些实施方案中，所涵盖的细菌包含一种或多种编码OxIT或其功能片段或变体、OXS或其功能片段或变体、和OXC或其功能片段或变体的转基因。在某些实施方案中，细菌进一步包含一种或多种编码FCOCT或其功能片段或变体和/或ACOCT或其功能片段或变体的转基因。
在某些实施方案中，所涵盖的细菌包含一种或多种编码OxIT或其功能片段或变体、OXS或其功能片段或变体、第一OXC或其功能片段或变体、第二OXC或其功能片段或变体、FCOCT或其功能片段或变体、和/或ACOCT或其功能片段或变体的转基因。在某些实施方案中，所涵盖的细菌包含一种或多种编码OxIT或其功能片段或变体、第一OXS或其功能片段或变体、第二OXS或其功能片段或变体、OXC或其功能片段或变体、FCOCT或其功能片段或变体、和/或ACOCT或其功能片段或变体的转基因。在某些实施方案中，所涵盖的细菌包含一种或多种编码OxIT或其功能片段或变体、第一OXS或其功能片段或变体、第二OXS或其功能片段或变体、第一OXC或其功能片段或变体、第二OXC或其功能片段或变体、FCOCT或其功能片段或变体、和/或ACOCT或其功能片段或变体的转基因。
在某些实施方案中，所涵盖的细菌包含一种或多种编码产甲酸草酸杆菌(O.formigenes)OxIT、酿酒酵母(S.cerevisiae)或拟南芥(A.thaliana)OXS、酿酒酵母或产甲酸草酸杆菌OXC、大肠杆菌(E.coli)FCOCT和/或大肠杆菌ACOCT的转基因。例如，预期细菌可以包含：(i)一种或多种编码酿酒酵母OXS、酿酒酵母OXC和产甲酸草酸杆菌OxIT的转基因；(ii)一种或多种编码大肠杆菌FCOCT、产甲酸草酸杆菌OXC、酿酒酵母OXS和产甲酸草酸杆菌OxIT的转基因；(iii)一种或多种编码大肠杆菌FCOCT、产甲酸草酸杆菌OXC、酿酒酵母OXS、酿酒酵母OXC、大肠杆菌ACOCT和产甲酸草酸杆菌OxIT的转基因；(iv)或一种或多种编码大肠杆菌FCOCT、产甲酸草酸杆菌OXC、酿酒酵母OXS、酿酒酵母OXC、拟南芥OXS、大肠杆菌ACOCT和产甲酸草酸杆菌OxIT的转基因；(v)一种或多种编码大肠杆菌FCOCT、产甲酸草酸杆菌OXC、产甲酸草酸杆菌OxIT、大肠杆菌ACOCT和酿酒酵母OXS的转基因；(vi)一种或多种编码产甲酸草酸杆菌OxIT、大肠杆菌FCOCT、酿酒酵母OXC、大肠杆菌ACOCT、酿酒酵母OXS和拟南芥OXS的转基因；或(vii)一种或多种编码产甲酸草酸杆菌OxIT、大肠杆菌FCOCT、酿酒酵母OXC、大肠杆菌ACOCT和酿酒酵母OXS的转基因。
在某些实施方案中，所涵盖的细菌包含一种或多种核酸，所述核酸包含与SEQ IDNO:1-31中的任一个具有至少80％同一性的核苷酸序列，或包含SEQ ID NO:1-31中的任一个的核苷酸序列。
例如，所涵盖的细菌可以包含：(i)一种或多种核酸，所述核酸包含与SEQ ID NO:16具有至少80％同一性的核苷酸序列、与SEQ ID NO:26或SEQ ID NO:163具有至少80％同一性的核苷酸序列和与SEQ ID NO:21具有至少80％同一性的核苷酸序列；(ii)一种或多种核酸，所述核酸包含与SEQ ID NO:7具有至少80％同一性的核苷酸序列、与SEQ ID NO:25具有至少80％同一性的核苷酸序列、与SEQ ID NO:16具有至少80％同一性的核苷酸序列和与SEQ ID NO:21具有至少80％同一性的核苷酸序列；(iii)一种或多种核酸，所述核酸包含与SEQ ID NO:7具有至少80％同一性的核苷酸序列、与SEQ ID NO:25具有至少80％同一性的核苷酸序列、与SEQ ID NO:16具有至少80％同一性的核苷酸序列、与SEQ ID NO:26或SEQID NO:163具有至少80％同一性的核苷酸序列、与SEQ ID NO:2具有至少80％同一性的核苷酸序列和与SEQ ID NO:21具有至少80％同一性的核苷酸序列；(iv)一种或多种核酸，所述核酸包含与SEQ ID NO:7具有至少80％同一性的核苷酸序列、与SEQ ID NO:25具有至少80％同一性的核苷酸序列、与SEQ ID NO:16具有至少80％同一性的核苷酸序列、与SEQ IDNO:26或SEQ ID NO:163具有至少80％同一性的核苷酸序列、与SEQ ID NO:14具有至少80％同一性的核苷酸序列、与SEQ ID NO:2具有至少80％同一性的核苷酸序列和与SEQ ID NO:21具有至少80％同一性的核苷酸序列；(v)一种或多种核酸，所述核酸包含与SEQ ID NO:7具有至少80％同一性的核苷酸序列、与SEQ ID NO:25具有至少80％同一性的核苷酸序列、与SEQ ID NO:21具有至少80％同一性的核苷酸序列、与SEQ ID NO:2具有至少80％同一性的核苷酸序列和与SEQ ID NO:16具有至少80％同一性的核苷酸序列；(vi)一种或多种核酸，所述核酸包含与SEQ ID NO:21具有至少80％同一性的核苷酸序列、与SEQ ID NO:7具有至少80％同一性的核苷酸序列、与SEQ ID NO:26或SEQ ID NO:163具有至少80％同一性的核苷酸序列、与SEQ ID NO:2具有至少80％同一性的核苷酸序列、与SEQ ID NO:16具有至少80％同一性的核苷酸序列和与SEQ ID NO:14具有至少80％同一性的核苷酸序列；或(vii)一种或多种核酸，所述核酸包含与SEQ ID NO:21具有至少80％同一性的核苷酸序列、与SEQID NO:7具有至少80％同一性的核苷酸序列、与SEQ ID NO:26或SEQ ID NO:163具有至少80％同一性的核苷酸序列、与SEQ ID NO:2具有至少80％同一性的核苷酸序列和与SEQ IDNO:16具有至少80％同一性的核苷酸序列。
例如，所涵盖的细菌可以包含：(i)一种或多种核酸，所述核酸包含SEQ ID NO:16、SEQ ID NO:26或SEQ ID NO:163、和SEQ ID NO:21的核苷酸序列；(ii)一种或多种核酸，所述核酸包含SEQ ID NO:7、SEQ ID NO:25、SEQ ID NO:16、和SEQ ID NO:21的核苷酸序列；(iii)一种或多种核酸，所述核酸包含SEQ ID NO:7、SEQ ID NO:25、SEQ ID NO:16、SEQ IDNO:26或SEQ ID NO:163、SEQ ID NO:2、和SEQ ID NO:21的核苷酸序列；(iv)一种或多种核酸，所述核酸包含SEQ ID NO:7、SEQ ID NO:25、SEQ ID NO:16、SEQ ID NO:26或SEQ ID NO:163、SEQ ID NO:14、SEQ ID NO:2、和SEQ ID NO:21的核苷酸序列；(v)一种或多种核酸，所述核酸包含SEQ ID NO:7、SEQ ID NO:25、SEQ ID NO:21、SEQ ID NO:2、和SEQ ID NO:16的核苷酸序列；(vi)一种或多种核酸，所述核酸包含SEQ ID NO:21、SEQ ID NO:7、SEQ ID NO:26或SEQ ID NO:163、SEQ ID NO:2、SEQ ID NO:16、和SEQ ID NO:14的核苷酸序列；或(vii)一种或多种核酸，所述核酸包含SEQ ID NO:21、SEQ ID NO:7、SEQ ID NO:26或SEQ ID NO:163、SEQ ID NO:2、和SEQ ID NO:16的核苷酸序列。
在某些实施方案中，将转基因或核酸可操作地连接至核糖体结合位点(RBS)。示例性的RBS包括包含SEQ ID NO:164-230中的任一个的核苷酸序列的那些。例如，所涵盖的细菌可以包含：(i)编码产甲酸草酸杆菌OxIT的转基因，其可操作地连接至包含SEQ ID NO:212-219中的任一个的核苷酸序列的RBS；(ii)编码酿酒酵母OXS的转基因，其可操作地连接至包含SEQ ID NO:172-179中的任一个的核苷酸序列的RBS；(iii)编码拟南芥OXS的转基因，其可操作地连接至包含SEQ ID NO:164-171中的任一个的核苷酸序列的RBS；(iv)编码酿酒酵母OXC的转基因，其可操作地连接至包含SEQ ID NO:188-195或220-230中的任一个的核苷酸序列的RBS；(v)编码产甲酸草酸杆菌OXC的转基因，其可操作地连接至包含SEQ IDNO:180-187中的任一个的核苷酸序列的RBS；(vi)编码大肠杆菌FCOCT的转基因，其可操作地连接至包含SEQ ID NO:204-211中的任一个的核苷酸序列的RBS；和/或(vii)编码大肠杆菌ACOCT的转基因，其可操作地连接至包含SEQ ID NO:196-203中的任一个的核苷酸序列的RBS。
在某些实施方案中，所涵盖的细菌包含一种或多种核酸，所述核酸包含与SEQ IDNO:96-162中的任一个具有至少80％同一性的核苷酸序列，或包含SEQ ID NO:96-162中的任一个的核苷酸序列。
例如，所涵盖的细菌可以包含：(i)一种或多种核酸，所述核酸包含与SEQ ID NO:137具有至少80％同一性的核苷酸序列、与SEQ ID NO:113具有至少80％同一性的核苷酸序列、与SEQ ID NO:145具有至少80％同一性的核苷酸序列、与SEQ ID NO:128具有至少80％同一性的核苷酸序列和与SEQ ID NO:104具有至少80％同一性的核苷酸序列；(ii)一种或多种核酸，所述核酸包含与SEQ ID NO:155具有至少80％同一性的核苷酸序列、与SEQ IDNO:137具有至少80％同一性的核苷酸序列、与SEQ ID NO:128具有至少80％同一性的核苷酸序列、与SEQ ID NO:148具有至少80％同一性的核苷酸序列、与SEQ ID NO:115具有至少80％同一性的核苷酸序列、与SEQ ID NO:98具有至少80％同一性的核苷酸序列和与SEQ IDNO:105具有至少80％同一性的核苷酸序列；(iii)一种或多种核酸，所述核酸包含与SEQ IDNO:155具有至少80％同一性的核苷酸序列、与SEQ ID NO:137具有至少80％同一性的核苷酸序列、与SEQ ID NO:128具有至少80％同一性的核苷酸序列、与SEQ ID NO:144具有至少80％同一性的核苷酸序列、与SEQ ID NO:115具有至少80％同一性的核苷酸序列、与SEQ IDNO:98具有至少80％同一性的核苷酸序列和与SEQ ID NO:105具有至少80％同一性的核苷酸序列；(iv)一种或多种核酸，所述核酸包含与SEQ ID NO:147具有至少80％同一性的核苷酸序列、与SEQ ID NO:104具有至少80％同一性的核苷酸序列、与SEQ ID NO:153具有至少80％同一性的核苷酸序列、与SEQ ID NO:97具有至少80％同一性的核苷酸序列、与SEQ IDNO:131具有至少80％同一性的核苷酸序列、与SEQ ID NO:136具有至少80％同一性的核苷酸序列和与SEQ ID NO:113具有至少80％同一性的核苷酸序列；(v)一种或多种核酸，所述核酸包含与SEQ ID NO:145具有至少80％同一性的核苷酸序列、与SEQ ID NO:137具有至少80％同一性的核苷酸序列、与SEQ ID NO:153具有至少80％同一性的核苷酸序列、与SEQ IDNO:129具有至少80％同一性的核苷酸序列、与SEQ ID NO:105具有至少80％同一性的核苷酸序列、与SEQ ID NO:113具有至少80％同一性的核苷酸序列和与SEQ ID NO:96具有至少80％同一性的核苷酸序列；(vi)一种或多种核酸，所述核酸包含与SEQ ID NO:145具有至少80％同一性的核苷酸序列、与SEQ ID NO:137具有至少80％同一性的核苷酸序列、与SEQ IDNO:153具有至少80％同一性的核苷酸序列、与SEQ ID NO:129具有至少80％同一性的核苷酸序列、与SEQ ID NO:105具有至少80％同一性的核苷酸序列和与SEQ ID NO:96具有至少80％同一性的核苷酸序列；(vii)一种或多种核酸，所述核酸包含与SEQ ID NO:139具有至少80％同一性的核苷酸序列、与SEQ ID NO:129具有至少80％同一性的核苷酸序列、与SEQID NO:155具有至少80％同一性的核苷酸序列、与SEQ ID NO:112具有至少80％同一性的核苷酸序列、与SEQ ID NO:99具有至少80％同一性的核苷酸序列、与SEQ ID NO:106具有至少80％同一性的核苷酸序列和与SEQ ID NO:145具有至少80％同一性的核苷酸序列；(viii)一种或多种核酸，所述核酸包含与SEQ ID NO:139具有至少80％同一性的核苷酸序列、与SEQ ID NO:154具有至少80％同一性的核苷酸序列、与SEQ ID NO:131具有至少80％同一性的核苷酸序列、与SEQ ID NO:98具有至少80％同一性的核苷酸序列、与SEQ ID NO:115具有至少80％同一性的核苷酸序列、与SEQ ID NO:106具有至少80％同一性的核苷酸序列和与SEQ ID NO:149具有至少80％同一性的核苷酸序列；(ix)一种或多种核酸，所述核酸包含与SEQ ID NO:137具有至少80％同一性的核苷酸序列、与SEQ ID NO:113具有至少80％同一性的核苷酸序列、与SEQ ID NO:148具有至少80％同一性的核苷酸序列、与SEQ ID NO:129具有至少80％同一性的核苷酸序列和与SEQ ID NO:105具有至少80％同一性的核苷酸序列；(x)一种或多种核酸，所述核酸包含与SEQ ID NO:145具有至少80％同一性的核苷酸序列、与SEQ ID NO:137具有至少80％同一性的核苷酸序列、与SEQ ID NO:153具有至少80％同一性的核苷酸序列、与SEQ ID NO:129具有至少80％同一性的核苷酸序列和与SEQ ID NO:105具有至少80％同一性的核苷酸序列；(xi)一种或多种核酸，所述核酸包含与SEQ ID NO:155具有至少80％同一性的核苷酸序列、与SEQ ID NO:137具有至少80％同一性的核苷酸序列、与SEQ ID NO:128具有至少80％同一性的核苷酸序列、与SEQ ID NO:144具有至少80％同一性的核苷酸序列、与SEQ ID NO:115具有至少80％同一性的核苷酸序列、与SEQ ID NO:98具有至少80％同一性的核苷酸序列和与SEQ ID NO:105具有至少80％同一性的核苷酸序列；(xii)一种或多种核酸，所述核酸包含与SEQ ID NO:145具有至少80％同一性的核苷酸序列、与SEQ ID NO:137具有至少80％同一性的核苷酸序列、与SEQ ID NO:153具有至少80％同一性的核苷酸序列、与SEQ ID NO:129具有至少80％同一性的核苷酸序列、与SEQ ID NO:105具有至少80％同一性的核苷酸序列、与SEQ ID NO:113具有至少80％同一性的核苷酸序列和与SEQ ID NO:96具有至少80％同一性的核苷酸序列；(xiii)一种或多种核酸，所述核酸包含与SEQ ID NO:145具有至少80％同一性的核苷酸序列、与SEQ ID NO:137具有至少80％同一性的核苷酸序列、与SEQ ID NO:153具有至少80％同一性的核苷酸序列、与SEQ IDNO:129具有至少80％同一性的核苷酸序列、与SEQ ID NO:105具有至少80％同一性的核苷酸序列和与SEQ ID NO:96具有至少80％同一性的核苷酸序列；或(xiv)一种或多种核酸，所述核酸包含与SEQ ID NO:139具有至少80％同一性的核苷酸序列、与SEQ ID NO:129具有至少80％同一性的核苷酸序列、与SEQ ID NO:155具有至少80％同一性的核苷酸序列、与SEQID NO:112具有至少80％同一性的核苷酸序列、与SEQ ID NO:99具有至少80％同一性的核苷酸序列、与SEQ ID NO:106具有至少80％同一性的核苷酸序列和与SEQ ID NO:145具有至少80％同一性的核苷酸序列。
例如，所涵盖的细菌可以包含：(i)一种或多种核酸，所述核酸包含SEQ ID NO:137、SEQ ID NO:113、SEQ ID NO:145、SEQ ID NO:128、和SEQ ID NO:104的核苷酸序列；(ii)一种或多种核酸，所述核酸包含SEQ ID NO:155、SEQ ID NO:137、SEQ ID NO:128、SEQID NO:148、SEQ ID NO:115、SEQ ID NO:98、和SEQ ID NO:105的核苷酸序列；(iii)一种或多种核酸，所述核酸包含SEQ ID NO:155、SEQ ID NO:137、SEQ ID NO:128、SEQ ID NO:144、SEQ ID NO:115、SEQ ID NO:98、和SEQ ID NO:105的核苷酸序列；(iv)一种或多种核酸，所述核酸包含SEQ ID NO:147、SEQ ID NO:104、SEQ ID NO:153、SEQ ID NO:97、SEQ ID NO:131、SEQ ID NO:136、和SEQ ID NO:113的核苷酸序列；(v)一种或多种核酸，所述核酸包含SEQ ID NO:145、SEQ ID NO:137、SEQ ID NO:153、SEQ ID NO:129、SEQ ID NO:105、SEQ IDNO:113、和SEQ ID NO:96的核苷酸序列；(vi)一种或多种核酸，所述核酸包含SEQ ID NO:145、SEQ ID NO:137、SEQ ID NO:153、SEQ ID NO:129、SEQ ID NO:105、和SEQ ID NO:96的核苷酸序列；(vii)一种或多种核酸，所述核酸包含SEQ ID NO:139、SEQ ID NO:129、SEQ IDNO:155、SEQ ID NO:112、SEQ ID NO:99、SEQ ID NO:106、和SEQ ID NO:145的核苷酸序列；(viii)一种或多种核酸，所述核酸包含SEQ ID NO:139、SEQ ID NO:154、SEQ ID NO:131、SEQ ID NO:98、SEQ ID NO:115、SEQ ID NO:106、和SEQ ID NO:149的核苷酸序列；(ix)一种或多种核酸，所述核酸包含SEQ ID NO:137、SEQ ID NO:113、SEQ ID NO:148、SEQ ID NO:129、和SEQ ID NO:105的核苷酸序列；(x)一种或多种核酸，所述核酸包含SEQ ID NO:145、SEQ ID NO:137、SEQ ID NO:153、SEQ ID NO:129、和SEQ ID NO:105的核苷酸序列；(xi)一种或多种核酸，所述核酸包含SEQ ID NO:155、SEQ ID NO:137、SEQ ID NO:128、SEQ ID NO:144、SEQ ID NO:115、SEQ ID NO:98、和SEQ ID NO:105的核苷酸序列；(xii)一种或多种核酸，所述核酸包含SEQ ID NO:145、SEQ ID NO:137、SEQ ID NO:153、SEQ ID NO:129、SEQ IDNO:105、SEQ ID NO:113、和SEQ ID NO:96的核苷酸序列；(xiii)一种或多种核酸，所述核酸包含SEQ ID NO:145、SEQ ID NO:137、SEQ ID NO:153、SEQ ID NO:129、SEQ ID NO:105、和SEQ ID NO:96的核苷酸序列；或(xiv)一种或多种核酸，所述核酸包含SEQ ID NO:139、SEQID NO:129、SEQ ID NO:155、SEQ ID NO:112、SEQ ID NO:99、SEQ ID NO:106、和SEQ ID NO:145的核苷酸序列。
在某些实施方案中，所涵盖的细菌包含一种或多种核酸，所述核酸包含与SEQ IDNO:82-95中的任一个具有至少80％同一性的核苷酸序列，或包含SEQ ID NO:82-95中的任一个的核苷酸序列。
在某些实施方案中，将转基因或核酸可操作地连接至至少一个组成型启动子，例如，噬菌体衍生的启动子。示例性的启动子包括包含共有序列GTTAA(n)4-7GTTAA(n)34-38TA(n)2TTTG(SEQ ID NO:70)、或包含SEQ ID NO:68、SEQ ID NO:69或SEQ ID NO:71的那些。
在某些实施方案中，所涵盖的细菌已被修饰成与未经修饰的类似的或在其它方面相同的细菌相比以增加的丰度、稳定性、预测性或初始定殖的容易性定殖人肠道。例如，可以修饰所涵盖的细菌以增加其利用特殊营养物(privileged nutrient)作为碳源的能力。例如，所涵盖的细菌可包含一种或多种转基因，所述转基因增加所述细菌利用特殊营养物作为碳源的能力。示例性的特殊营养物包括例如海洋多糖，例如紫菜多糖(porphyran)。所公开的细菌可以例如在施用于人受试者后产生每克粪便含量大于1012、1011、1010、109、108或107cfu的丰度。
在另一个方面，本文提供了包含公开的细菌和药学上可接受的赋形剂的药物组合物。在某些实施方案中，将所涵盖的药物组合物配制为胶囊或片剂，例如，肠溶衣胶囊。在某些实施方案中，所涵盖的药物组合物进一步包含特殊营养物，例如，海洋多糖，例如，紫菜多糖。
在另一个方面，本文提供了在有需要的受试者中减少草酸盐的方法。所涵盖的方法包括给所述受试者施用有效量的公开的细菌或药物组合物。在某些实施方案中，受试者具有与草酸盐量升高相关的病症，例如，高草酸尿症。在另一个方面，本文提供了治疗受试者中与草酸盐量升高相关的病症(例如，高草酸尿症)的方法。所涵盖的方法包括给所述受试者施用有效量的公开的细菌或药物组合物。
所涵盖的方法可以包括每12小时、24小时、1天、2天、3天、4天、5天、6天、7天、1周、2周、3周、4周、1个月、2个月、3个月、4个月、5个月或6个月给受试者施用公开的细菌或药物组合物。在某些实施方案中，公开的细菌或药物组合物向受试者的连续施用之间的时间大于48小时。
所涵盖的方法可以进一步包括向受试者施用特殊营养物，例如，海洋多糖，例如，紫菜多糖。例如，可以在公开的细菌之前、同时或之后将公开的特殊营养物施用给受试者。
在另一个方面，本发明提供了编码蛋白或其功能片段或变体的表达载体，所述蛋白选自：草酸盐:甲酸盐反向转运蛋白(OxlT)；草酸脱羧酶(OXDC)-EC 4.1.1.2；草酸氧化酶(OXO)-EC 1.2.3.4；草酸氧化还原酶(OOR)-EC 1.2.7.10；草酸辅酶A连接酶/草酰辅酶A合成酶(OXS)-EC 6.2.1.8；甲酰辅酶A:草酸辅酶A转移酶(FCOCT)-EC 2.8.3.16；乙酰辅酶A:草酸辅酶A转移酶(ACOCT)-EC 2.8.3.19；琥珀酰辅酶A:草酸辅酶A转移酶(SCOCT)-EC2.8.3.2；草酰辅酶A脱羧酶(OXC)-EC 4.1.1.8；草酰辅酶A还原酶/乙醛酸盐:NADP+氧化还原酶(OXR)-EC 1.2.1.17；和前述蛋白任何的组合。示例性的表达载体包括，例如，质粒和细菌人工染色体。当表达载体包含一种或多种编码多种蛋白的转基因时，预期编码两种或更多种蛋白的开放读码框可以例如存在于单个操纵子中。
在某些实施方案中，所涵盖的表达载体包含一种或多种编码OxIT或其功能片段或变体、OXS或其功能片段或变体、和OXC或其功能片段或变体的转基因。在某些实施方案中，表达载体进一步包含一种或多种编码FCOCT或其功能片段或变体、和/或ACOCT或其功能片段或变体的转基因。
在某些实施方案中，所涵盖的表达载体包含一种或多种编码OxIT或其功能片段或变体、OXS或其功能片段或变体、第一OXC或其功能片段或变体、第二OXC或其功能片段或变体、FCOCT或其功能片段或变体、和/或ACOCT或其功能片段或变体的转基因。在某些实施方案中，所涵盖的表达载体包含一种或多种编码OxIT或其功能片段或变体、第一OXS或其功能片段或变体、第二OXS或其功能片段或变体、OXC或其功能片段或变体、FCOCT或其功能片段或变体、和/或ACOCT或其功能片段或变体的转基因。在某些实施方案中，所涵盖的表达载体包含一种或多种编码OxIT或其功能片段或变体、第一OXS或其功能片段或变体、第二OXS或其功能片段或变体、第一OXC或其功能片段或变体、第二OXC或其功能片段或变体、FCOCT或其功能片段或变体、和/或ACOCT或其功能片段或变体的转基因。
在某些实施方案中，所涵盖的表达载体包含一种或多种编码产甲酸草酸杆菌OxIT、酿酒酵母或拟南芥OXS、酿酒酵母或产甲酸草酸杆菌OXC、大肠杆菌FCOCT、和/或大肠杆菌ACOCT的转基因。例如，预期表达载体可以包含：(i)一种或多种编码酿酒酵母OXS、酿酒酵母OXC和产甲酸草酸杆菌OxIT的转基因；(ii)一种或多种编码大肠杆菌FCOCT、产甲酸草酸杆菌OXC、酿酒酵母OXS和产甲酸草酸杆菌OxIT的转基因；(iii)一种或多种编码大肠杆菌FCOCT、产甲酸草酸杆菌OXC、酿酒酵母OXS、酿酒酵母OXC、大肠杆菌ACOCT和产甲酸草酸杆菌OxIT的转基因；(iv)或一种或多种编码大肠杆菌FCOCT、产甲酸草酸杆菌OXC、酿酒酵母OXS、酿酒酵母OXC、拟南芥OXS、大肠杆菌ACOCT和产甲酸草酸杆菌OxIT的转基因；(v)一种或多种编码大肠杆菌FCOCT、产甲酸草酸杆菌OXC、产甲酸草酸杆菌OxIT、大肠杆菌ACOCT和酿酒酵母OXS的转基因；(vi)一种或多种编码产甲酸草酸杆菌OxIT、大肠杆菌FCOCT、酿酒酵母OXC、大肠杆菌ACOCT、酿酒酵母OXS和拟南芥OXS的转基因；或(vii)一种或多种编码产甲酸草酸杆菌OxIT、大肠杆菌FCOCT、酿酒酵母OXC、大肠杆菌ACOCT和酿酒酵母OXS的转基因。
在某些实施方案中，所涵盖的表达载体包含一种或多种核酸，所述核酸包含与SEQID NO:1-31中的任一个具有至少80％同一性的核苷酸序列，或包含SEQ ID NO:1-31中的任一个的核苷酸序列。
例如，所涵盖的表达载体可以包含：(i)一种或多种核酸，所述核酸包含与SEQ IDNO:16具有至少80％同一性的核苷酸序列、与SEQ ID NO:26或SEQ ID NO:163具有至少80％同一性的核苷酸序列和与SEQ ID NO:21具有至少80％同一性的核苷酸序列；(ii)一种或多种核酸，所述核酸包含与SEQ ID NO:7具有至少80％同一性的核苷酸序列、与SEQ ID NO:25具有至少80％同一性的核苷酸序列、与SEQ ID NO:16具有至少80％同一性的核苷酸序列和与SEQ ID NO:21具有至少80％同一性的核苷酸序列；(iii)一种或多种核酸，所述核酸包含与SEQ ID NO:7具有至少80％同一性的核苷酸序列、与SEQ ID NO:25具有至少80％同一性的核苷酸序列、与SEQ ID NO:16具有至少80％同一性的核苷酸序列、与SEQ ID NO:26或SEQID NO:163具有至少80％同一性的核苷酸序列、与SEQ ID NO:2具有至少80％同一性的核苷酸序列和与SEQ ID NO:21具有至少80％同一性的核苷酸序列；(iv)一种或多种核酸，所述核酸包含与SEQ ID NO:7具有至少80％同一性的核苷酸序列、与SEQ ID NO:25具有至少80％同一性的核苷酸序列、与SEQ ID NO:16具有至少80％同一性的核苷酸序列、与SEQ IDNO:26或SEQ ID NO:163具有至少80％同一性的核苷酸序列、与SEQ ID NO:14具有至少80％同一性的核苷酸序列、与SEQ ID NO:2具有至少80％同一性的核苷酸序列和与SEQ ID NO:21具有至少80％同一性的核苷酸序列；(v)一种或多种核酸，所述核酸包含与SEQ ID NO:7具有至少80％同一性的核苷酸序列、与SEQ ID NO:25具有至少80％同一性的核苷酸序列、与SEQ ID NO:21具有至少80％同一性的核苷酸序列、与SEQ ID NO:2具有至少80％同一性的核苷酸序列和与SEQ ID NO:16具有至少80％同一性的核苷酸序列；(vi)一种或多种核酸，所述核酸包含与SEQ ID NO:21具有至少80％同一性的核苷酸序列、与SEQ ID NO:7具有至少80％同一性的核苷酸序列、与SEQ ID NO:26或SEQ ID NO:163具有至少80％同一性的核苷酸序列、与SEQ ID NO:2具有至少80％同一性的核苷酸序列、与SEQ ID NO:16具有至少80％同一性的核苷酸序列和与SEQ ID NO:14具有至少80％同一性的核苷酸序列；或(vii)一种或多种核酸，所述核酸包含与SEQ ID NO:21具有至少80％同一性的核苷酸序列、与SEQID NO:7具有至少80％同一性的核苷酸序列、与SEQ ID NO:26或SEQ ID NO:163具有至少80％同一性的核苷酸序列、与SEQ ID NO:2具有至少80％同一性的核苷酸序列和与SEQ IDNO:16具有至少80％同一性的核苷酸序列。
例如，所涵盖的表达载体可以包含：(i)一种或多种核酸，所述核酸包含SEQ IDNO:16、SEQ ID NO:26或SEQ ID NO:163、和SEQ ID NO:21的核苷酸序列；(ii)一种或多种核酸，所述核酸包含SEQ ID NO:7、SEQ ID NO:25、SEQ ID NO:16、和SEQ ID NO:21的核苷酸序列；(iii)一种或多种核酸，所述核酸包含SEQ ID NO:7、SEQ ID NO:25、SEQ ID NO:16、SEQID NO:26或SEQ ID NO:163、SEQ ID NO:2、和SEQ ID NO:21的核苷酸序列；(iv)一种或多种核酸，所述核酸包含SEQ ID NO:7、SEQ ID NO:25、SEQ ID NO:16、SEQ ID NO:26或SEQ IDNO:163、SEQ ID NO:14、SEQ ID NO:2、和SEQ ID NO:21的核苷酸序列；(v)一种或多种核酸，所述核酸包含SEQ ID NO:7、SEQ ID NO:25、SEQ ID NO:21、SEQ ID NO:2、和SEQ ID NO:16的核苷酸序列；(vi)一种或多种核酸，所述核酸包含SEQ ID NO:21、SEQ ID NO:7、SEQ IDNO:26或SEQ ID NO:163、SEQ ID NO:2、SEQ ID NO:16、和SEQ ID NO:14的核苷酸序列；或(vii)一种或多种核酸，所述核酸包含SEQ ID NO:21、SEQ ID NO:7、SEQ ID NO:26或SEQ IDNO:163、SEQ ID NO:2、和SEQ ID NO:16的核苷酸序列。
在某些实施方案中，将转基因或核酸可操作地连接至核糖体结合位点(RBS)。示例性的RBS包括包含SEQ ID NO:164-230中的任一个的核苷酸序列的那些。例如，所涵盖的表达载体可以包含：(i)编码产甲酸草酸杆菌OxIT的转基因，其可操作地连接至包含SEQ IDNO:212-219中的任一个的核苷酸序列的RBS；(ii)编码酿酒酵母OXS的转基因，其可操作地连接至包含SEQ ID NO:172-179中的任一个的核苷酸序列的RBS；(iii)编码拟南芥OXS的转基因，其可操作地连接至包含SEQ ID NO:164-171中的任一个的核苷酸序列的RBS；(iv)编码酿酒酵母OXC的转基因，其可操作地连接至包含SEQ ID NO:188-195或220-230中的任一个的核苷酸序列的RBS；(v)编码产甲酸草酸杆菌OXC的转基因，其可操作地连接至包含SEQID NO:180-187中的任一个的核苷酸序列的RBS；(vi)编码大肠杆菌FCOCT的转基因，其可操作地连接至包含SEQ ID NO:204-211中的任一个的核苷酸序列的RBS；和/或(vii)编码大肠杆菌ACOCT的转基因，其可操作地连接至包含SEQ ID NO:196-203中的任一个的核苷酸序列的RBS。
在某些实施方案中，所涵盖的表达载体包含一种或多种核酸，所述核酸包含与SEQID NO:96-162中的任一个具有至少80％同一性的核苷酸序列，或包含SEQ ID NO:96-162中的任一个的核苷酸序列。
例如，所涵盖的表达载体可以包含：(i)一种或多种核酸，所述核酸包含与SEQ IDNO:137具有至少80％同一性的核苷酸序列、与SEQ ID NO:113具有至少80％同一性的核苷酸序列、与SEQ ID NO:145具有至少80％同一性的核苷酸序列、与SEQ ID NO:128具有至少80％同一性的核苷酸序列和与SEQ ID NO:104具有至少80％同一性的核苷酸序列；(ii)一种或多种核酸，所述核酸包含与SEQ ID NO:155具有至少80％同一性的核苷酸序列、与SEQID NO:137具有至少80％同一性的核苷酸序列、与SEQ ID NO:128具有至少80％同一性的核苷酸序列、与SEQ ID NO:148具有至少80％同一性的核苷酸序列、与SEQ ID NO:115具有至少80％同一性的核苷酸序列、与SEQ ID NO:98具有至少80％同一性的核苷酸序列和与SEQID NO:105具有至少80％同一性的核苷酸序列；(iii)一种或多种核酸，所述核酸包含与SEQID NO:155具有至少80％同一性的核苷酸序列、与SEQ ID NO:137具有至少80％同一性的核苷酸序列、与SEQ ID NO:128具有至少80％同一性的核苷酸序列、与SEQ ID NO:144具有至少80％同一性的核苷酸序列、与SEQ ID NO:115具有至少80％同一性的核苷酸序列、与SEQID NO:98具有至少80％同一性的核苷酸序列和与SEQ ID NO:105具有至少80％同一性的核苷酸序列；(iv)一种或多种核酸，所述核酸包含与SEQ ID NO:147具有至少80％同一性的核苷酸序列、与SEQ ID NO:104具有至少80％同一性的核苷酸序列、与SEQ ID NO:153具有至少80％同一性的核苷酸序列、与SEQ ID NO:97具有至少80％同一性的核苷酸序列、与SEQID NO:131具有至少80％同一性的核苷酸序列、与SEQ ID NO:136具有至少80％同一性的核苷酸序列和与SEQ ID NO:113具有至少80％同一性的核苷酸序列；(v)一种或多种核酸，所述核酸包含与SEQ ID NO:145具有至少80％同一性的核苷酸序列、与SEQ ID NO:137具有至少80％同一性的核苷酸序列、与SEQ ID NO:153具有至少80％同一性的核苷酸序列、与SEQID NO:129具有至少80％同一性的核苷酸序列、与SEQ ID NO:105具有至少80％同一性的核苷酸序列、与SEQ ID NO:113具有至少80％同一性的核苷酸序列和与SEQ ID NO:96具有至少80％同一性的核苷酸序列；(vi)一种或多种核酸，所述核酸包含与SEQ ID NO:145具有至少80％同一性的核苷酸序列、与SEQ ID NO:137具有至少80％同一性的核苷酸序列、与SEQID NO:153具有至少80％同一性的核苷酸序列、与SEQ ID NO:129具有至少80％同一性的核苷酸序列、与SEQ ID NO:105具有至少80％同一性的核苷酸序列和与SEQ ID NO:96具有至少80％同一性的核苷酸序列；(vii)一种或多种核酸，所述核酸包含与SEQ ID NO:139具有至少80％同一性的核苷酸序列、与SEQ ID NO:129具有至少80％同一性的核苷酸序列、与SEQ ID NO:155具有至少80％同一性的核苷酸序列、与SEQ ID NO:112具有至少80％同一性的核苷酸序列、与SEQ ID NO:99具有至少80％同一性的核苷酸序列、与SEQ ID NO:106具有至少80％同一性的核苷酸序列和与SEQ ID NO:145具有至少80％同一性的核苷酸序列；(viii)一种或多种核酸，所述核酸包含与SEQ ID NO:139具有至少80％同一性的核苷酸序列、与SEQ ID NO:154具有至少80％同一性的核苷酸序列、与SEQ ID NO:131具有至少80％同一性的核苷酸序列、与SEQ ID NO:98具有至少80％同一性的核苷酸序列、与SEQ ID NO:115具有至少80％同一性的核苷酸序列、与SEQ ID NO:106具有至少80％同一性的核苷酸序列和与SEQ ID NO:149具有至少80％同一性的核苷酸序列；(ix)一种或多种核酸，所述核酸包含与SEQ ID NO:137具有至少80％同一性的核苷酸序列、与SEQ ID NO:113具有至少80％同一性的核苷酸序列、与SEQ ID NO:148具有至少80％同一性的核苷酸序列、与SEQ ID NO:129具有至少80％同一性的核苷酸序列和与SEQ ID NO:105具有至少80％同一性的核苷酸序列；(x)一种或多种核酸，所述核酸包含与SEQ ID NO:145具有至少80％同一性的核苷酸序列、与SEQ ID NO:137具有至少80％同一性的核苷酸序列、与SEQ ID NO:153具有至少80％同一性的核苷酸序列、与SEQ ID NO:129具有至少80％同一性的核苷酸序列和与SEQID NO:105具有至少80％同一性的核苷酸序列；(xi)一种或多种核酸，所述核酸包含与SEQID NO:155具有至少80％同一性的核苷酸序列、与SEQ ID NO:137具有至少80％同一性的核苷酸序列、与SEQ ID NO:128具有至少80％同一性的核苷酸序列、与SEQ ID NO:144具有至少80％同一性的核苷酸序列、与SEQ ID NO:115具有至少80％同一性的核苷酸序列、与SEQID NO:98具有至少80％同一性的核苷酸序列和与SEQ ID NO:105具有至少80％同一性的核苷酸序列；(xii)一种或多种核酸，所述核酸包含与SEQ ID NO:145具有至少80％同一性的核苷酸序列、与SEQ ID NO:137具有至少80％同一性的核苷酸序列、与SEQ ID NO:153具有至少80％同一性的核苷酸序列、与SEQ ID NO:129具有至少80％同一性的核苷酸序列、与SEQ ID NO:105具有至少80％同一性的核苷酸序列、与SEQ ID NO:113具有至少80％同一性的核苷酸序列和与SEQ ID NO:96具有至少80％同一性的核苷酸序列；(xiii)一种或多种核酸，所述核酸包含与SEQ ID NO:145具有至少80％同一性的核苷酸序列、与SEQ ID NO:137具有至少80％同一性的核苷酸序列、与SEQ ID NO:153具有至少80％同一性的核苷酸序列、与SEQ ID NO:129具有至少80％同一性的核苷酸序列、与SEQ ID NO:105具有至少80％同一性的核苷酸序列和与SEQ ID NO:96具有至少80％同一性的核苷酸序列；或(xiv)一种或多种核酸，所述核酸包含与SEQ ID NO:139具有至少80％同一性的核苷酸序列、与SEQ ID NO:129具有至少80％同一性的核苷酸序列、与SEQ ID NO:155具有至少80％同一性的核苷酸序列、与SEQ ID NO:112具有至少80％同一性的核苷酸序列、与SEQ ID NO:99具有至少80％同一性的核苷酸序列、与SEQ ID NO:106具有至少80％同一性的核苷酸序列和与SEQ ID NO:145具有至少80％同一性的核苷酸序列。
例如，所涵盖的表达载体可以包含：(i)一种或多种核酸，所述核酸包含SEQ IDNO:137、SEQ ID NO:113、SEQ ID NO:145、SEQ ID NO:128、和SEQ ID NO:104的核苷酸序列；(ii)一种或多种核酸，所述核酸包含SEQ ID NO:155、SEQ ID NO:137、SEQ ID NO:128、SEQID NO:148、SEQ ID NO:115、SEQ ID NO:98、和SEQ ID NO:105的核苷酸序列；(iii)一种或多种核酸，所述核酸包含SEQ ID NO:155、SEQ ID NO:137、SEQ ID NO:128、SEQ ID NO:144、SEQ ID NO:115、SEQ ID NO:98、和SEQ ID NO:105的核苷酸序列；(iv)一种或多种核酸，所述核酸包含SEQ ID NO:147、SEQ ID NO:104、SEQ ID NO:153、SEQ ID NO:97、SEQ ID NO:131、SEQ ID NO:136、和SEQ ID NO:113的核苷酸序列；(v)一种或多种核酸，所述核酸包含SEQ ID NO:145、SEQ ID NO:137、SEQ ID NO:153、SEQ ID NO:129、SEQ ID NO:105、SEQ IDNO:113、和SEQ ID NO:96的核苷酸序列；(vi)一种或多种核酸，所述核酸包含SEQ ID NO:145、SEQ ID NO:137、SEQ ID NO:153、SEQ ID NO:129、SEQ ID NO:105、和SEQ ID NO:96的核苷酸序列；(vii)一种或多种核酸，所述核酸包含SEQ ID NO:139、SEQ ID NO:129、SEQ IDNO:155、SEQ ID NO:112、SEQ ID NO:99、SEQ ID NO:106、和SEQ ID NO:145的核苷酸序列；(viii)一种或多种核酸，所述核酸包含SEQ ID NO:139、SEQ ID NO:154、SEQ ID NO:131、SEQ ID NO:98、SEQ ID NO:115、SEQ ID NO:106、和SEQ ID NO:149的核苷酸序列；(ix)一种或多种核酸，所述核酸包含SEQ ID NO:137、SEQ ID NO:113、SEQ ID NO:148、SEQ ID NO:129、和SEQ ID NO:105的核苷酸序列；(x)一种或多种核酸，所述核酸包含SEQ ID NO:145、SEQ ID NO:137、SEQ ID NO:153、SEQ ID NO:129、和SEQ ID NO:105的核苷酸序列；(xi)一种或多种核酸，所述核酸包含SEQ ID NO:155、SEQ ID NO:137、SEQ ID NO:128、SEQ ID NO:144、SEQ ID NO:115、SEQ ID NO:98、和SEQ ID NO:105的核苷酸序列；(xii)一种或多种核酸，所述核酸包含SEQ ID NO:145、SEQ ID NO:137、SEQ ID NO:153、SEQ ID NO:129、SEQ IDNO:105、SEQ ID NO:113、和SEQ ID NO:96的核苷酸序列；(xiii)一种或多种核酸，所述核酸包含SEQ ID NO:145、SEQ ID NO:137、SEQ ID NO:153、SEQ ID NO:129、SEQ ID NO:105、和SEQ ID NO:96的核苷酸序列；或(xiv)一种或多种核酸，所述核酸包含SEQ ID NO:139、SEQID NO:129、SEQ ID NO:155、SEQ ID NO:112、SEQ ID NO:99、SEQ ID NO:106、和SEQ ID NO:145的核苷酸序列。
在某些实施方案中，所涵盖的表达载体包含一种或多种核酸，所述核酸包含与SEQID NO:82-95中的任一个具有至少80％同一性的核苷酸序列，或包含SEQ ID NO:82-95中的任一个的核苷酸序列。
在某些实施方案中，将转基因或核酸可操作地连接至至少一个组成型启动子，例如，噬菌体衍生的启动子。示例性的启动子包括包含共有序列GTTAA(n)4-7GTTAA(n)34-38TA(n)2TTTG(SEQ ID NO:70)、或包含SEQ ID NO:68、SEQ ID NO:69或SEQ ID NO:71的那些。
在以下详细描述和权利要求中描述本公开内容的这些和其它方面和特征。
附图说明
参考下述附图可以更完全地理解本公开内容。
图1的示意图描绘了草酸盐降解的途径。使用OxIT草酸盐:甲酸盐反向转运蛋白将草酸盐输入细菌细胞中，然后经由多种不同途径转化成甲酸盐、乙醛酸盐或CO2。最高产途径使用OXS、FCOCT、ACOCT或SCOCT将草酸盐与辅酶A缀合以制备草酰辅酶A，然后使用OXC将草酰辅酶A脱羧为甲酰辅酶A。然后使用FCOCT释放甲酸盐并重新开始循环。
图2的饼形图显示了基于16S核糖体DNA测序的普通人肠道中的细菌丰度。拟杆菌属是最丰富的属，占46％。
图3描绘了5种不同质粒(SEQ ID NO:63-67)的示意图，它们可以缀合到拟杆菌属中以产生降解草酸盐的拟杆菌属菌株。
图4的条形图描绘了普通拟杆菌(Bacteroides vulgatus)ATCC 8482消耗的草酸盐的量，该菌没有转基因(野生型)，或具有以下质粒的基因组整合：pZR748(SEQ ID NO:63)、pZR753(SEQ ID NO:64)、pZR753+pZR761(SEQ ID NO:64、65)或pZR856+pZR878(SEQ IDNO:66、67)。将每个拟杆菌属菌株的过夜培养物以1:10稀释到补充有10或30mM草酸盐的新鲜BHIS液体培养基中，并在48小时内监测草酸盐消耗速率。
图5描绘了质粒pWD035(SEQ ID NO:73)的示意图，该质粒是带有接合转移起点和紫菜多糖的多糖利用基因座(PUL)的细菌人工染色体。
图6描绘了接受功能性紫菜多糖PUL(pWD035，SEQ ID NO:73)的普通拟杆菌细胞与野生型普通拟杆菌细胞相比的体外生长测定。将饱和培养物在补充有0.2％紫菜多糖的基本培养基中稀释50倍，并在37℃厌氧生长14小时。全程监测培养物的光密度。只有含有紫菜多糖PUL的细胞才能在紫菜多糖上生长。
图7描绘了携带草酸盐消耗基因的三种不同质粒(SEQ ID NO:75-77)。每种质粒都缀合到利用紫菜多糖的菌株NB075中以产生可以消耗特殊营养物紫菜多糖和肠道衍生的毒素草酸盐的菌株。
图8的线图描绘了在将菌株以1010cfu/ml添加到含有4mM草酸盐的新鲜BHIS培养基中之后指定的经工程改造的拟杆菌属菌株的草酸盐消耗速率。
图9的线图描绘了在将菌株以1010cfu/ml添加到含有4mM草酸盐的来自Sprague-Dawley大鼠的盲肠内容物以后，指定的经工程改造的拟杆菌属菌株的草酸盐消耗速率。
图10的条形图描绘了在用指定菌株处理后在继发性高草酸尿症的大鼠模型中的尿草酸盐水平。相对于不消耗草酸盐的对照菌株(NB075)，消耗草酸盐的菌株sWW152在菌株施用后4-6天产生32％的尿草酸盐减少(p＝0.005)。误差棒代表95％置信区间。
图11的线图描绘了在用指定菌株处理后继发性高草酸尿症的大鼠模型中的尿草酸盐水平。相对于不消耗草酸盐的对照菌株(NB124)，消耗草酸盐的菌株sWW554在菌株施用后4-6天产生46％的尿草酸盐减少(p＝0.0003)。阴影区域代表95％置信区间。
图12的条形图描绘了在图11中总结的实验第12天的每种菌株的粪便丰度。
图13A的条形图描绘了在用指定菌株和指定量(重量百分比)的紫菜多糖(在饮用水中施用)处理后在继发性高草酸尿症的大鼠模型中的菌株丰度(通过每克粪便的菌落形成单位(CFU)来测量)。图13B的条形图描绘了在用指定菌株和指定量(重量百分比)的紫菜多糖(在饮用水中施用)处理后在继发性高草酸尿症的大鼠模型中的尿草酸盐水平。条代表平均值，点代表单个动物，且误差棒代表95％置信区间。
图14的条形图描绘了在用指定菌株处理后通过Route-en-Y胃旁路(RYGB)手术诱导的高草酸尿症大鼠中的尿草酸盐水平。条代表每组大鼠的每日总尿草酸盐的最后连续五天平均值。误差棒代表95％置信区间(n＝5只假处理大鼠，所有其它组n＝8)。
图15A描绘了核糖体结合位点(RBS)的筛选结果。如实施例8中所述，每个点代表具有不同的经工程改造的RBS的每个指定基因的表达(通过log10发光值测量)。图15B描绘了具有指定RBS的指定基因的表达(通过发光测量)。误差棒代表95％置信区间。
图16的条形图描绘了指定菌株的体外草酸盐降解。误差棒代表95％置信区间。
具体实施方式
已知人肠道(特别是结肠)可渗透草酸盐。它是饮食草酸盐吸收的主要部位，且有人认为，草酸盐从血流中分泌出来并进入肠腔会提供从体内清除草酸盐的次要途径。因此，结肠是降解草酸盐的系统的有吸引力的位置，从而防止饮食草酸盐的吸收和分泌的内源性草酸盐的重吸收。
本公开内容总体上涉及已被修饰成具有增加的草酸盐降解活性的细菌。例如，在一个方面，本文提供了一种共生细菌，其已被修饰成与未经修饰的类似的或在其它方面相同的细菌相比具有增加的草酸盐降解活性。预期所公开的细菌在施用于受试者后可分解在受试者中(例如在受试者的大肠中)的草酸盐，且因此可用于治疗受试者中与草酸盐量升高相关的病症，例如，高草酸尿症。因此，本公开内容进一步涉及使用公开的细菌治疗与草酸盐量升高相关的病症(例如高草酸尿症)的药物组合物或单元和方法。
所涵盖的经修饰的细菌可另外具有利用碳源(诸如海洋多糖紫菜多糖(porphyrin))的能力，在待治疗受试者的肠道中的其它细菌在很大程度上不能利用所述碳源。结果，通过向受试者供应碳源可以维持受试者的肠道中经修饰的细菌的增殖、丰度或稳定性。
I.经修饰的细菌
本公开内容总体上涉及已被修饰成与未经修饰的类似的或在其它方面相同的细菌相比具有增加的草酸盐降解活性的细菌。例如，可以修饰所考虑的细菌以表达一种或多种增加草酸盐降解活性的转基因。术语“草酸盐降解”在本文中与术语“草酸盐消耗”互换使用。在图1中描绘了草酸盐降解的途径，包括与草酸盐降解相关的基因。预期一种或多种转基因可以，例如，在质粒、细菌人工染色体上，或整合在基因组中。当细菌包含一种或多种编码多种蛋白的转基因时，预期编码两种或更多种蛋白的开放读码框可以例如存在于单个操纵子中。
示例性的与草酸盐降解相关的基因(其在细菌中的表达可增加草酸盐降解活性)包括编码以下酶的那些：草酸盐:甲酸盐反向转运蛋白(OxlT)；草酸脱羧酶(OXDC)-EC4.1.1.2；草酸氧化酶(OXO)-EC 1.2.3.4；草酸氧化还原酶(OOR)-EC 1.2.7.10；草酸辅酶A连接酶/草酰辅酶A合成酶(OXS)-EC 6.2.1.8；甲酰辅酶A:草酸辅酶A转移酶(FCOCT)-EC2.8.3.16；乙酰辅酶A:草酸辅酶A转移酶(ACOCT)-EC 2.8.3.19；琥珀酰辅酶A:草酸辅酶A转移酶(SCOCT)-EC 2.8.3.2；草酰辅酶A脱羧酶(OXC)-EC 4.1.1.8；和草酰辅酶A还原酶/乙醛酸盐:NADP+氧化还原酶(OXR)-EC 1.2.1.17。
为了使细菌降解草酸盐，可能需要表达草酸盐转运蛋白，通常是草酸盐:甲酸盐反向转运蛋白。示例性的草酸盐转运蛋白包括来自产甲酸草酸杆菌的OxlT或其来自其它物种的任何同系物，所述其它物种包括草酸盐贪铜菌(Cupriavidus oxalaticus)和动物双歧杆菌乳酸亚种(Bifidobacterium animalis subsp.lactis)。这些反向转运蛋白将草酸盐从肠道环境带入细菌细胞，且作为交换，每带入一分子的草酸盐，就会排出一分子的甲酸盐废物。一旦草酸盐进入细胞，就可以使用草酸脱羧酶(OXDC)-EC 4.1.1.2、草酸氧化酶(OXO)-EC 1.2.3.4或草酸氧化还原酶(OOR)-EC 1.2.7.10将该草酸盐直接降解。
备选地，使用草酸盐--辅酶A连接酶(OXS)-EC 6.2.1.8、甲酰辅酶A:草酸辅酶A转移酶(FCOCT)-EC 2.8.3.16、乙酰辅酶A:草酸辅酶A转移酶(ACOCT)-EC 2.8.3.19或琥珀酰辅酶A:草酸辅酶A转移酶(SCOCT)-EC 2.8.3.2，可以将草酸盐缀合至辅酶A以产生草酰辅酶A。FCOCT使用甲酰辅酶A作为辅酶A供体，并且随着甲酰辅酶A在草酰辅酶A降解过程中作为废物产生，导致有效循环。还可能需要使用更丰富的起始原料诸如ATP、乙酰辅酶A或琥珀酰辅酶A(分别被OXS、ACOCT和SCOCT使用)开始所述循环。
草酰辅酶A被草酰辅酶A脱羧酶(OXC)-EC 4.1.1.8分解。这又产生甲酰辅酶A，其被FCOCT降解以释放甲酸盐。然后甲酸盐可被甲酸脱氢酶(FDH)-EC 1.2.1.2降解以产生CO2。草酰辅酶A处理的另一个选择是通过草酰辅酶A还原酶(OXR)/乙醛酸盐:NADP+氧化还原酶-EC1.2.1.17还原成乙醛酸盐以构建额外的生物质。
在所涵盖的经修饰的细菌的某些实施方案中，使用OxlT反向转运蛋白将草酸盐输入细菌中，并加入使用FCOCT和OXC的草酸盐到甲酸盐转化的连续循环中。通过OXC酶的多个拷贝的表达增加，可以提供对该循环的进一步支持。此外，可以通过OXS、ACOCT或SCOCT的表达来创建和支持循环中间体草酰辅酶A和甲酰辅酶A的池。因此，在某些实施方案中，所涵盖的细菌细胞具有OXC和FCOCT的高多拷贝表达，OXS、ACOCT和/或SCOCT的高表达，以及OxlT的中度表达。在某些实施方案中，草酸盐:甲酸盐反向转运蛋白的N-端片段已与来自天然转运蛋白的N-端片段交换。
因此，在某些实施方案中，所涵盖的细菌包含一种或多种编码OxIT或其功能片段或变体、OXS或其功能片段或变体、和OXC或其功能片段或变体的转基因。在某些实施方案中，细菌进一步包含一种或多种编码FCOCT或其功能片段或变体、和/或ACOCT或其功能片段或变体的转基因。
在某些实施方案中，所涵盖的细菌包含一种或多种编码OxIT或其功能片段或变体、OXS或其功能片段或变体、第一OXC或其功能片段或变体、第二OXC或其功能片段或变体、FCOCT或其功能片段或变体、和/或ACOCT或其功能片段或变体的转基因。在某些实施方案中，所涵盖的细菌包含一种或多种编码OxIT或其功能片段或变体、第一OXS或其功能片段或变体、第二OXS或其功能片段或变体、OXC或其功能片段或变体、FCOCT或其功能片段或变体、和/或ACOCT或其功能片段或变体的转基因。在某些实施方案中，所涵盖的细菌包含一种或多种编码OxIT或其功能片段或变体、第一OXS或其功能片段或变体、第二OXS或其功能片段或变体、第一OXC或其功能片段或变体、第二OXC或其功能片段或变体、FCOCT或其功能片段或变体、和/或ACOCT或其功能片段或变体的转基因。
本文中使用的术语生物实体的“功能片段”(例如，基因、蛋白(例如，OxIT、OXS、OXC或FCOCT)、启动子或核糖体结合位点)表示全长生物实体的片段，其保留了例如对应的全长的、天然存在的生物实体的生物活性的至少10％、至少20％、至少30％、至少40％、至少50％、至少60％、至少70％、至少80％、至少90％或100％。
在某些实施方案中，所涵盖的细菌包含一种或多种编码动物双歧杆菌乳酸亚种OxIT(例如，动物双歧杆菌乳酸亚种DSM 10140OxIT)、草酸盐贪铜菌OxIT(例如，草酸盐贪铜菌ATCC 11883OxIT)、大肠杆菌OxIT(例如，大肠杆菌MG1655 OxIT)、产甲酸草酸杆菌OxIT(例如，产甲酸草酸杆菌DSM 4420OxIT)或任何前述蛋白的功能片段或变体的转基因。例如，在某些实施方案中，所涵盖的细菌包含一种或多种编码产甲酸草酸杆菌OxIT(例如，产甲酸草酸杆菌DSM 4420OxIT)或与产甲酸草酸杆菌OxIT具有至少80％、85％、86％、87％、88％、89％、90％、91％、92％、93％、94％、95％、96％、97％、98％或99％同一性的蛋白的转基因。
在某些实施方案中，所涵盖的细菌包含一种或多种编码酿酒酵母OXS(例如，酿酒酵母S288C OXS)、扭脱甲基杆菌(M.extorquens)OXS(例如，扭脱甲基杆菌AM1/DSM1338OXS)、拟南芥OXS(例如，拟南芥col-1OXS)或任何前述蛋白的功能片段或变体的转基因。例如，在某些实施方案中，所涵盖的细菌包含一种或多种编码酿酒酵母OXS(例如，酿酒酵母S288C OXS)或与酿酒酵母OXS具有至少80％、85％、86％、87％、88％、89％、90％、91％、92％、93％、94％、95％、96％、97％、98％或99％同一性的蛋白的转基因。在某些实施方案中，所涵盖的细菌包含一种或多种编码拟南芥OXS(例如，拟南芥col-1OXS)或与拟南芥OXS具有至少80％、85％、86％、87％、88％、89％、90％、91％、92％、93％、94％、95％、96％、97％、98％或99％同一性的蛋白的转基因。
在某些实施方案中，所涵盖的细菌包含一种或多种编码动物双歧杆菌乳酸亚种OXC(例如，动物双歧杆菌乳酸亚种DSM 10140OXC)、草酸盐贪铜菌OXC(例如，草酸盐贪铜菌ATCC 11883OXC)、大肠杆菌OXC(例如，大肠杆菌MG1655 OXC)、酿酒酵母OXC(例如，酿酒酵母S288C OXC)、产甲酸草酸杆菌OXC(例如，产甲酸草酸杆菌DSM 4420OXC)或任何前述蛋白的功能片段或变体的转基因。例如，在某些实施方案中，所涵盖的细菌包含一种或多种编码酿酒酵母OXC(例如，酿酒酵母S288C OXC)或与酿酒酵母OXC具有至少80％、85％、86％、87％、88％、89％、90％、91％、92％、93％、94％、95％、96％、97％、98％或99％同一性的蛋白的转基因。在某些实施方案中，所涵盖的细菌包含一种或多种编码产甲酸草酸杆菌OXC(例如，产甲酸草酸杆菌DSM4420OXC)或与产甲酸草酸杆菌OXC具有至少80％、85％、86％、87％、88％、89％、90％、91％、92％、93％、94％、95％、96％、97％、98％或99％同一性的蛋白的转基因。
在某些实施方案中，所涵盖的细菌包含一种或多种编码动物双歧杆菌乳酸亚种FCOCT(例如，动物双歧杆菌乳酸亚种DSM 10140FCOCT)、草酸盐贪铜菌FCOCT(例如，草酸盐贪铜菌ATCC 11883FCOCT)、大肠杆菌FCOCT(例如，大肠杆菌MG1655 FCOCT)、产甲酸草酸杆菌FCOCT(例如，产甲酸草酸杆菌DSM 4420FCOCT)或任何前述蛋白的功能片段或变体的转基因。例如，在某些实施方案中，所涵盖的细菌包含一种或多种编码大肠杆菌FCOCT(例如，大肠杆菌MG1655 FCOCT)或与大肠杆菌FCOCT具有至少80％、85％、86％、87％、88％、89％、90％、91％、92％、93％、94％、95％、96％、97％、98％或99％同一性的蛋白的转基因。
在某些实施方案中，所涵盖的细菌包含一种或多种编码醋化醋杆菌(A.aceti)ACOCT(例如，醋化醋杆菌菌株1023ACOCT)、大肠杆菌ACOCT(例如，大肠杆菌MG1655 ACOCT)或任何前述蛋白的功能片段或变体的转基因。例如，在某些实施方案中，所涵盖的细菌包含一种或多种编码大肠杆菌ACOCT(例如，大肠杆菌MG1655 ACOCT)或与大肠杆菌ACOCT具有至少80％、85％、86％、87％、88％、89％、90％、91％、92％、93％、94％、95％、96％、97％、98％或99％同一性的蛋白的转基因。
例如，预期细菌可以包含：(i)一种或多种编码酿酒酵母OXS、酿酒酵母OXC和产甲酸草酸杆菌OxIT或任何前述蛋白的功能片段或变体的转基因；(ii)一种或多种编码大肠杆菌FCOCT、产甲酸草酸杆菌OXC、酿酒酵母OXS和产甲酸草酸杆菌OxIT或任何前述蛋白的功能片段或变体的转基因；(iii)一种或多种编码大肠杆菌FCOCT、产甲酸草酸杆菌OXC、酿酒酵母OXS、酿酒酵母OXC、大肠杆菌ACOCT和产甲酸草酸杆菌OxIT或任何前述蛋白的功能片段或变体的转基因；(iv)一种或多种编码大肠杆菌FCOCT、产甲酸草酸杆菌OXC、酿酒酵母OXS、酿酒酵母OXC、拟南芥OXS、大肠杆菌ACOCT和产甲酸草酸杆菌OxIT或任何前述蛋白的功能片段或变体的转基因；(v)一种或多种编码大肠杆菌FCOCT、产甲酸草酸杆菌OXC、产甲酸草酸杆菌OxIT、大肠杆菌ACOCT和酿酒酵母OXS或任何前述蛋白的功能片段或变体的转基因；(vi)一种或多种编码产甲酸草酸杆菌OxIT、大肠杆菌FCOCT、酿酒酵母OXC、大肠杆菌ACOCT、酿酒酵母OXS和拟南芥OXS或任何前述蛋白的功能片段或变体的转基因；或(vii)一种或多种编码产甲酸草酸杆菌OxIT、大肠杆菌FCOCT、酿酒酵母OXC、大肠杆菌ACOCT和酿酒酵母OXS或任何前述蛋白的功能片段或变体的转基因。
示例性的ACOCT编码序列描述在SEQ ID NO:1-2中，示例性的FDH编码序列描述在SEQ ID NO:3-4中，示例性的FCOCT编码序列描述在SEQ ID NO:4-8中，一个示例性的OXDC编码序列描述在SEQ ID NO:9中，一个示例性的OXO编码序列描述在SEQ ID NO:10中，示例性的OOR编码序列描述在SEQ ID NO:11-13中，示例性的OXS编码序列描述在SEQ ID NO:14-16中，示例性的OxIT编码序列描述在SEQ ID NO:17-21中，示例性的OXC编码序列描述在SEQID NO:22-26和163中，一个示例性的OXR编码序列描述在SEQ ID NO:27中，和示例性的SCOCT编码序列描述在SEQ ID NO:28-31中。
因此，在某些实施方案中，细菌已被修饰成包含一种或多种核酸，所述核酸包含SEQ ID NO:1-31中的任一个的核苷酸序列或其功能片段或变体。在某些实施方案中，细菌已被修饰成包含一种或多种核酸，所述核酸包含与SEQ ID NO:1-31中的任一个具有至少80％、85％、86％、87％、88％、89％、90％、91％、92％、93％、94％、95％、96％、97％、98％或99％同一性的核苷酸序列或其功能片段或变体。
例如，预期细菌可以包含一种或多种核酸，所述核酸包含SEQ ID NO:16、SEQ IDNO:26或SEQ ID NO:163、和SEQ ID NO:21的核苷酸序列，或与SEQ ID NO:16、SEQ ID NO:26或SEQ ID NO:163、和SEQ ID NO:21具有至少80％、85％、86％、87％、88％、89％、90％、91％、92％、93％、94％、95％、96％、97％、98％或99％同一性的核苷酸序列。预期细菌可以包含一种或多种核酸，所述核酸包含SEQ ID NO:7、SEQ ID NO:25、SEQ ID NO:16和SEQ IDNO:21的核苷酸序列，或与SEQ ID NO:7、SEQ ID NO:25、SEQ ID NO:16和SEQ ID NO:21具有至少80％、85％、86％、87％、88％、89％、90％、91％、92％、93％、94％、95％、96％、97％、98％或99％同一性的核苷酸序列。预期细菌可以包含一种或多种核酸，所述核酸包含SEQID NO:7、SEQ ID NO:25、SEQ ID NO:16、SEQ ID NO:26或SEQ ID NO:163、SEQ ID NO:2和SEQ ID NO:21的核苷酸序列，或与SEQ ID NO:7、SEQ ID NO:25、SEQ ID NO:16、SEQ ID NO:26或SEQ ID NO:163、SEQ ID NO:2和SEQ ID NO:21具有至少80％、85％、86％、87％、88％、89％、90％、91％、92％、93％、94％、95％、96％、97％、98％或99％同一性的核苷酸序列。预期细菌可以包含一种或多种核酸，所述核酸包含SEQ ID NO:7、SEQ ID NO:25、SEQ ID NO:16、SEQ ID NO:26或SEQ ID NO:163、SEQ ID NO:14、SEQ ID NO:2和SEQ ID NO:21的核苷酸序列，或与SEQ ID NO:7、SEQ ID NO:25、SEQ ID NO:16、SEQ ID NO:26或SEQ ID NO:163、SEQ ID NO:14、SEQ ID NO:2和SEQ ID NO:21具有至少80％、85％、86％、87％、88％、89％、90％、91％、92％、93％、94％、95％、96％、97％、98％或99％同一性的核苷酸序列。预期细菌可以包含一种或多种核酸，所述核酸包含SEQ ID NO:7、SEQ ID NO:25、SEQ ID NO:21、SEQID NO:2和SEQ ID NO:16的核苷酸序列，或与SEQ ID NO:7、SEQ ID NO:25、SEQ ID NO:21、SEQ ID NO:2和SEQ ID NO:16具有至少80％、85％、86％、87％、88％、89％、90％、91％、92％、93％、94％、95％、96％、97％、98％或99％同一性的核苷酸序列。预期细菌可以包含一种或多种核酸，所述核酸包含SEQ ID NO:21、SEQ ID NO:7、SEQ ID NO:26或SEQ ID NO:163、SEQ ID NO:2、SEQ ID NO:16和SEQ ID NO:14的核苷酸序列，或与SEQ ID NO:21、SEQID NO:7、SEQ ID NO:26或SEQ ID NO:163、SEQ ID NO:2、SEQ ID NO:16和SEQ ID NO:14具有至少80％、85％、86％、87％、88％、89％、90％、91％、92％、93％、94％、95％、96％、97％、98％或99％同一性的核苷酸序列。预期细菌可以包含一种或多种核酸，所述核酸包含SEQID NO:21、SEQ ID NO:7、SEQ ID NO:26或SEQ ID NO:163、SEQ ID NO:2和SEQ ID NO:16的核苷酸序列，或与SEQ ID NO:21、SEQ ID NO:7、SEQ ID NO:26或SEQ ID NO:163、SEQ IDNO:2和SEQ ID NO:16具有至少80％、85％、86％、87％、88％、89％、90％、91％、92％、93％、94％、95％、96％、97％、98％或99％同一性的核苷酸序列。
在某些实施方案中，细菌已被修饰成包含一种或多种核酸，所述核酸包含编码SEQID NO:32-62中的任一个的氨基酸序列的核苷酸序列或其功能片段或变体。在某些实施方案中，细菌已被修饰成包含一种或多种核酸，所述核酸包含编码与SEQ ID NO:32-62中的任一个具有至少80％、85％、86％、87％、88％、89％、90％、91％、92％、93％、94％、95％、96％、97％、98％或99％同一性的氨基酸序列的核苷酸序列或其功能片段或变体。
例如，预期细菌可以包含一种或多种核酸，所述核酸包含一种或多种核苷酸序列，所述核苷酸序列编码SEQ ID NO:47、SEQ ID NO:57和SEQ ID NO:52的氨基酸序列，或与SEQID NO:47、SEQ ID NO:57和SEQ ID NO:52具有至少80％、85％、86％、87％、88％、89％、90％、91％、92％、93％、94％、95％、96％、97％、98％或99％同一性的氨基酸序列。预期细菌可以包含一种或多种核酸，所述核酸包含一种或多种核苷酸序列，所述核苷酸序列编码SEQID NO:38、SEQ ID NO:56、SEQ ID NO:47和SEQ ID NO:52的氨基酸序列，或与SEQ ID NO:38、SEQ ID NO:56、SEQ ID NO:47和SEQ ID NO:52具有至少80％、85％、86％、87％、88％、89％、90％、91％、92％、93％、94％、95％、96％、97％、98％或99％同一性的氨基酸序列。预期细菌可以包含一种或多种核酸，所述核酸包含一种或多种核苷酸序列，所述核苷酸序列编码SEQ ID NO:38、SEQ ID NO:56、SEQ ID NO:47、SEQ ID NO:57、SEQ ID NO:33和SEQ IDNO:52的氨基酸序列，或与SEQ ID NO:38、SEQ ID NO:56、SEQ ID NO:47、SEQ ID NO:57、SEQID NO:33和SEQ ID NO:52具有至少80％、85％、86％、87％、88％、89％、90％、91％、92％、93％、94％、95％、96％、97％、98％或99％同一性的氨基酸序列。预期细菌可以包含一种或多种核酸，所述核酸包含一种或多种核苷酸序列，所述核苷酸序列编码SEQ ID NO:38、SEQID NO:56、SEQ ID NO:47、SEQ ID NO:57、SEQ ID NO:45、SEQ ID NO:33和SEQ ID NO:52的氨基酸序列，或与SEQ ID NO:38、SEQ ID NO:56、SEQ ID NO:47、SEQ ID NO:57、SEQ ID NO:45、SEQ ID NO:33和SEQ ID NO:52具有至少80％、85％、86％、87％、88％、89％、90％、91％、92％、93％、94％、95％、96％、97％、98％或99％同一性的氨基酸序列。预期细菌可以包含一种或多种核酸，所述核酸包含一种或多种核苷酸序列，所述核苷酸序列编码SEQ ID NO:38、SEQ ID NO:56、SEQ ID NO:52、SEQ ID NO:33和SEQ ID NO:47的氨基酸序列，或与SEQ IDNO:38、SEQ ID NO:56、SEQ ID NO:52、SEQ ID NO:33和SEQ ID NO:47具有至少80％、85％、86％、87％、88％、89％、90％、91％、92％、93％、94％、95％、96％、97％、98％或99％同一性的氨基酸序列。预期细菌可以包含一种或多种核酸，所述核酸包含一种或多种核苷酸序列，所述核苷酸序列编码SEQ ID NO:52、SEQ ID NO:38、SEQ ID NO:57、SEQ ID NO:33、SEQ IDNO:47和SEQ ID NO:45的氨基酸序列，或与SEQ ID NO:52、SEQ ID NO:38、SEQ ID NO:57、SEQ ID NO:33、SEQ ID NO:47和SEQ ID NO:45具有至少80％、85％、86％、87％、88％、89％、90％、91％、92％、93％、94％、95％、96％、97％、98％或99％同一性的氨基酸序列。预期细菌可以包含一种或多种核酸，所述核酸包含一种或多种核苷酸序列，所述核苷酸序列编码SEQID NO:52、SEQ ID NO:38、SEQ ID NO:57、SEQ ID NO:33和SEQ ID NO:47的氨基酸序列，或与SEQ ID NO:52、SEQ ID NO:38、SEQ ID NO:57SEQ ID NO:33和SEQ ID NO:47具有至少80％、85％、86％、87％、88％、89％、90％、91％、92％、93％、94％、95％、96％、97％、98％或99％同一性的氨基酸序列。
可以以本领域技术范围内的各种方式确定序列同一性，例如，使用公众可得到的计算机软件诸如BLAST、BLAST-2、ALIGN或Megalign(DNASTAR)软件。使用程序blastp、blastn、blastx、tblastn和tblastx所采用的算法(Karlin等人,(1990)PROC.NATL.ACAD.SCI.USA 87:2264-2268；Altschul,(1993)J.MOL.EVOL.36,290-300；Altschul等人,(1997)NUCLEIC ACIDS RES.25:3389-3402，通过引用并入)的BLAST(BasicLocal Alignment Search Tool，基本局部比对搜索工具)分析是为序列相似性搜索而量身定制。对于搜索序列数据库中的基本问题的讨论，参见Altschul等人,(1994)NATUREGENETICS 6:119-129，其全部内容通过引用并入。本领域技术人员可以确定用于测量比对的合适参数，包括在所对比的序列的全长上实现最大比对所需的任何算法。直方图、描述、比对、期望(即报告与数据库序列匹配的统计显著性阈值)、截止、矩阵和过滤器的搜索参数处于默认设置。blastp、blastx、tblastn和tblastx所使用的默认评分矩阵是BLOSUM62矩阵(Henikoff等人,(1992)PROC.NATL.ACAD.SCI.USA 89:10915-10919，全部通过引用并入)。四个blastn参数可以调整如下：Q＝10(缺口生成罚分)；R＝10(缺口延伸罚分)；wink＝1(沿着查询在每个wink.sup.th位置产生单词命中)；和gapw＝16(设定在其中产生有缺口比对的窗口宽度)。等效的Blastp参数设置可以是Q＝9；R＝2；wink＝1；和gapw＝32。也可以使用NCBI(美国国家生物技术信息中心)BLAST高级选项参数(例如:-G,打开缺口的代价[整数]:默认＝5(对于核苷酸)/11(对于蛋白)；-E,延伸缺口的代价[整数]:默认＝2(对于核苷酸)/1(对于蛋白)；-q,核苷酸错配的罚分[整数]:默认＝-3；-r,核苷酸匹配的奖励[整数]:默认＝1；-e,期望值[Real]:默认＝10；-W,单词大小[整数]:默认＝11(对于核苷酸)/28(对于megablast)/3(对于蛋白)；-y,在命中中的blast延伸的跌落(X):默认＝20(对于blastn)/7(对于其它)；-X，有缺口比对的X跌落值(在命中中):默认＝对于所有程序为15，不适用于blastn；和-Z,有缺口比对的最终X跌落值(在命中中):对于blastn为50，对于其它为25)进行搜索。也可以使用用于逐对蛋白比对的ClustalW(默认参数可以包括，例如，Blosum62矩阵和缺口开放罚分＝10和缺口延伸罚分＝0.1)。在GCG包10.0版中可得到的序列之间的最适(Bestfit)对比使用DNA参数GAP＝50(缺口生成罚分)和LEN＝3(缺口延伸罚分)，且在蛋白对比中的等效设置是GAP＝8和LEN＝2。
所涵盖的经修饰的细菌(例如，用在公开的药物组合物或方法中)包括拟杆菌属、另枝菌属、栖粪杆菌属、副拟杆菌属、普雷沃氏菌属、罗氏菌属、瘤胃球菌属、梭菌属、颤杆菌属、芽殖菌属、巴恩斯氏菌、戴阿利斯特杆菌属、副萨特氏菌属、考拉杆菌属、丙酸杆菌属、萨特氏菌属、布劳特氏菌属、副普雷沃氏菌属、粪球菌属、臭菌属(Odoribacter)、螺原体属、厌氧棒状菌属或阿克曼氏菌属的细菌。所涵盖的细菌(例如，用在公开的药物组合物或方法中)可以属于拟杆菌属，即，可以是拟杆菌属中各种的细菌。
示例性的拟杆菌属种包括产酸拟杆菌(B.acidifaciens)、B.barnesiaes、粪拟杆菌(B.caccae)、B.caecicola、B.caecigallinarum、解纤维拟杆菌(B.cellulosilyticus)、溶纤维素拟杆菌(B.cellulosolvens)、B.clarus、凝结拟杆菌(B.coagulans)、B.coprocola、嗜粪拟杆菌(B.coprophilus)、B.coprosuis、吉氏拟杆菌(B.distasonis)、多氏拟杆菌(B.dorei)、埃氏拟杆菌(B.eggerthii)、纤细拟杆菌(B.gracilis)、B.faecichinchillae、B.faecis、细金拟杆菌(B.finegoldii)、B.fluxus、脆弱拟杆菌(B.fragilis)、半乳糖醛酸拟杆菌(B.galacturonicus)、雉拟杆菌(B.gallinaceum)、鸡拟杆菌(B.gallinarum)、B.goldsteinii、B.graminisolvens、生溃疡拟杆菌(B.helcogene)、肠拟杆菌(B.intestinalis)、B.luti、马赛拟杆菌(B.massiliensis)、黑色素拟杆菌(B.melaninogenicus)、B.nordii、B.oleiciplenus、口腔拟杆菌(B.oris)、卵形拟杆菌(B.ovatus)、B.paurosaccharolyticus、嗜果胶拟杆菌(B.pectinophilus)、B.plebeius、多动拟杆菌(B.polypragmatus)、B.propionicifaciens、腐败拟杆菌(B.putredinis)、酿脓拟杆菌(B.pyogenes)、网状拟杆菌(B.reticulotermitis)、啮齿拟杆菌(B.rodentium)、B.salanitronis、B.salyersiae、B.sartorii、B.sedimenti、粪便拟杆菌(B.stercoris)、猪拟杆菌(B.suis)、隐蔽拟杆菌(B.tectus)、多形拟杆菌(B.thetaiotaomicron)、单形拟杆菌(B.uniformis)、普通拟杆菌(B.vulgatus)、木糖降解拟杆菌(B.xylanisolvens)和解木聚糖拟杆菌(B.xylanolyticusxylanolyticus)。
本文中使用的术语“物种”表示按常规由基因组序列和表型特征定义的分类实体。“菌株”是已根据常规微生物学技术分离和纯化的物种的特定实例。本公开内容涵盖所公开的细菌菌株的衍生物。术语“衍生物”包括子代菌株(后代)或从原始菌株培养(亚克隆)但在不负面改变菌株生物活性的情况下以某种方式(包括在遗传水平上)修改的菌株。
在某些实施方案中，所涵盖的经修饰的细菌属于占待治疗受试者的粪便中或普通人的粪便中的总可培养微生物的超过0.1％、0.5％、1％、5％、10％、20％、30％或40％的属。在某些实施方案中，所涵盖的经修饰的细菌属于以每克待治疗受试者粪便或每克普通人粪便大于1012、1011、1010、109、108、107个菌落形成单位的水平检测到的属。在某些实施方案中，所涵盖的经修饰的细菌属于占待治疗受试者的肠道微生物组或普通人的肠道微生物组的超过0.1％、0.5％、1％、5％、10％、20％、30％或40％的属。通过本领域已知的任何技术，包括16S核糖体测序，可以测定人肠道或粪便微生物组的组成。图2显示了基于16S核糖体DNA测序的普通人肠道中的细菌丰度。拟杆菌属是人肠道中天然含量最丰富的属。
rRNA、16S rDNA、16S rRNA、16S、18S、18S rRNA和18S rDNA表示作为核糖体组分的组分或编码核糖体组分的核酸。在核糖体中有两个亚基，称为小亚基(SSU)和大亚基(LSU)。rDNA基因及其互补的RNA序列被广泛用于确定生物体的进化关系数量，因为它们是可变的，但仍足够保守以允许跨生物体分子对比。
在某些实施方案中，30S SSU的16S rDNA序列(大约1542个核苷酸的长度)可用于原核生物的基于分子的分类学分配，且40S SSU的18S rDNA序列(大约1869个核苷酸的长度)可用于真核生物。例如，16S序列可用于系统发育重建，因为它们通常是高度保守的，但包含特定的高变区，所述高变区携带足够的核苷酸多样性以区分大多数细菌的属和种。尽管16S rDNA序列数据已被用于提供分类学分类，但归入同一属和种内的密切相关的细菌菌株可能表现出不同的生物学表型。
所涵盖的细菌物种或菌株的身份可以通过16S rRNA或全基因组序列分析来表征。例如，在某些实施方案中，所涵盖的细菌菌株可包含与参考序列具有一定百分比(％)同一性的16S rRNA或基因组序列。
在某些实施方案中，所涵盖的经修饰的细菌能够稳定定殖人肠道。所公开的细菌可以例如在施用于人受试者后产生每克粪便含量大于1012、1011、1010、109、108或107cfu的丰度。例如，将约103、约104、约105、约106、约107、约108、约109、约1010、约1011或约1012个公开的细菌细胞施用于人受试者可导致在施用12小时、24小时、36小时、48小时、60小时或72小时后每克粪便含量大于1012、1011、1010、109、108或107cfu的丰度。
所公开的细菌可以例如已经被修饰成与未经修饰的类似的或在其它方面相同的细菌相比以增加的丰度、稳定性、预测性或初始定殖的容易性定殖人肠道。例如，可以修饰所考虑的细菌以增加其利用特殊营养物作为碳源的能力。“特殊营养物”被定义为一种分子或一组分子，其可被消耗以辅助特定细菌菌株的增殖，同时为肠道中不超过1％的其它细菌提供增殖辅助。因此，在某些实施方案中，即使在没有草酸盐或其它碳源或能源存在下，经修饰的细菌也能够消耗特殊营养物以维持其在受试者肠道中的定殖和繁殖至可预测的高丰度，而在受试者肠道中的大多数其它细菌不能。示例性的特殊营养物包括，例如，海洋多糖，例如，紫菜多糖。
例如，细菌可包含一种或多种转基因，所述转基因增加其利用特殊营养物(例如，海洋多糖，例如，紫菜多糖(porphyrin))作为碳源的能力。在某些实施方案中，细菌可以包含多糖利用基因座(PUL)的全部或部分，所述多糖利用基因座是赋予细菌消耗碳水化合物(例如特殊营养物)的能力的移动遗传元件。示例性的紫菜多糖消耗PUL是来自在SEQ IDNO:72中描述的消耗紫菜多糖的拟杆菌属菌株NB001的PUL。因此，在某些实施方案中，经修饰的细菌包含SEQ ID NO:72或其功能片段或变体。在某些实施方案中，经修饰的细菌包含与SEQ ID NO:72具有至少80％、85％、86％、87％、88％、89％、90％、91％、92％、93％、94％、95％、96％、97％、98％或99％同一性的核苷酸序列或其功能片段或变体。
在国际(PCT)专利公开号WO2018112194中描述了用于增加受试者肠道丰度的其它示例性细菌修饰、特殊营养物、增加细菌利用特殊营养物的能力的转基因、PUL、以及用于调节经修饰的细菌的生长的其它方法和组合物。
在某些实施方案中，未经修饰的类似的或在其它方面相同的细菌不具有可检测的草酸盐降解活性和/或不具有已知参与(例如，直接参与)草酸盐分解代谢的基因。例如，未经修饰的类似的或在其它方面相同的细菌可以不具有编码以下酶的基因：草酸盐:甲酸盐反向转运蛋白(OxlT)；草酸脱羧酶(OXDC)-EC 4.1.1.2；草酸氧化酶(OXO)-EC 1.2.3.4；草酸氧化还原酶(OOR)-EC 1.2.7.10；草酸辅酶A连接酶/草酰辅酶A合成酶(OXS)-EC6.2.1.8；甲酰辅酶A:草酸辅酶A转移酶(FCOCT)-EC 2.8.3.16；乙酰辅酶A:草酸辅酶A转移酶(ACOCT)-EC 2.8.3.19；琥珀酰辅酶A:草酸辅酶A转移酶(SCOCT)-EC 2.8.3.2；草酰辅酶A脱羧酶(OXC)-EC 4.1.1.8；或草酰辅酶A还原酶/乙醛酸盐:NADP+氧化还原酶(OXR)-EC1.2.1.17。在某些实施方案中，与细菌相同属的天然存在的成员不具有可检测到的草酸盐降解活性。
在某些实施方案中，包含外源核苷酸序列的公开的转基因或核酸可操作地连接至至少一个组成型启动子，例如，噬菌体衍生的启动子。术语“可操作地连接”表示以功能关系连接的多核苷酸元件。当一个核酸序列被置于与另一个核酸序列的功能关系中时，它被“可操作地连接”。例如，如果启动子或增强子影响基因的转录，则启动子或增强子与基因可操作地连接。可操作地连接的核苷酸序列通常是连续的。但是，由于增强子通常在与启动子相隔几千个碱基时起作用并且内含子序列可能具有可变长度，因此一些多核苷酸元件可能可操作地连接但不直接侧接，且甚至可能从不同的等位基因或染色体以反式起作用。示例性的噬菌体衍生的启动子包括包含SEQ ID NO:68、SEQ ID NO:69、SEQ ID NO:70或SEQ IDNO:71的核苷酸序列的启动子，或包含与SEQ ID NO:68、SEQ ID NO:69、SEQ ID NO:70或SEQID NO:71具有至少80％、85％、86％、87％、88％、89％、90％、91％、92％、93％、94％、95％、96％、97％、98％或99％同一性的核苷酸序列的启动子。在国际(PCT)专利公开号WO2017184565中描述了其它示例性的噬菌体衍生的启动子。
在某些实施方案中，所公开的包含外源核苷酸序列的转基因或核酸可操作地连接至至少一个核糖体结合位点(RBS)。示例性的RBS包括包含SEQ ID NO:164-230中的任一个的核苷酸序列、与SEQ ID NO:164-230中的任一个具有至少80％、85％、86％、87％、88％、89％、90％、91％、92％、93％、94％、95％、96％、97％、98％或99％同一性的核苷酸序列、或前述核苷酸序列中的任一个的功能片段或变体的那些。例如，所涵盖的细菌可以包含：(i)编码产甲酸草酸杆菌OxIT的转基因，其可操作地连接至RBS，所述RBS包含SEQ ID NO:212-219中的任一个的核苷酸序列、与SEQ ID NO:212-219中的任一个具有至少80％、85％、86％、87％、88％、89％、90％、91％、92％、93％、94％、95％、96％、97％、98％或99％同一性的核苷酸序列、或前述核苷酸序列中的任一个的功能片段或变体；(ii)编码酿酒酵母OXS的转基因，其可操作地连接至RBS，所述RBS包含SEQ ID NO:172-179中的任一个的核苷酸序列、与SEQ ID NO:172-179中的任一个具有至少80％、85％、86％、87％、88％、89％、90％、91％、92％、93％、94％、95％、96％、97％、98％或99％同一性的核苷酸序列、或前述核苷酸序列中的任一个的功能片段或变体；(iii)编码拟南芥OXS的转基因，其可操作地连接至RBS，所述RBS包含SEQ ID NO:164-171中的任一个的核苷酸序列、与SEQ ID NO:164-171中的任一个具有至少80％、85％、86％、87％、88％、89％、90％、91％、92％、93％、94％、95％、96％、97％、98％或99％同一性的核苷酸序列、或前述核苷酸序列中的任一个的功能片段或变体；(iv)编码酿酒酵母OXC的转基因，其可操作地连接至RBS，所述RBS包含SEQ ID NO:188-195或220-230中的任一个的核苷酸序列、与SEQ ID NO:188-195或220-230中的任一个具有至少80％、85％、86％、87％、88％、89％、90％、91％、92％、93％、94％、95％、96％、97％、98％或99％同一性的核苷酸序列、或前述核苷酸序列中的任一个的功能片段或变体；(v)编码产甲酸草酸杆菌OXC的转基因，其可操作地连接至RBS，所述RBS包含SEQ ID NO:180-187中的任一个的核苷酸序列、与SEQ ID NO:180-187中的任一个具有至少80％、85％、86％、87％、88％、89％、90％、91％、92％、93％、94％、95％、96％、97％、98％或99％同一性的核苷酸序列、或前述核苷酸序列中的任一个的功能片段或变体；(vi)编码大肠杆菌FCOCT的转基因，其可操作地连接至RBS，所述RBS包含SEQ ID NO:204-211中的任一个的核苷酸序列、与SEQ ID NO:204-211中的任一个具有至少80％、85％、86％、87％、88％、89％、90％、91％、92％、93％、94％、95％、96％、97％、98％或99％同一性的核苷酸序列、或前述核苷酸序列中的任一个的功能片段或变体；和/或(vii)编码大肠杆菌ACOCT的转基因，其可操作地连接至RBS，所述RBS包含SEQ ID NO:196-203中的任一个的核苷酸序列、与SEQ ID NO:196-203中的任一个具有至少80％、85％、86％、87％、88％、89％、90％、91％、92％、93％、94％、95％、96％、97％、98％或99％同一性的核苷酸序列、或前述核苷酸序列中的任一个的功能片段或变体。
预期细菌可以包含一种或多种核酸，所述核酸包含SEQ ID NO:96-162中的任一个的核苷酸序列，或与SEQ ID NO:96-162中的任一个的核苷酸序列具有至少80％、85％、86％、87％、88％、89％、90％、91％、92％、93％、94％、95％、96％、97％、98％或99％同一性的核苷酸序列。
例如，预期细菌可以包含一种或多种核酸，所述核酸包含SEQ ID NO:137、SEQ IDNO:113、SEQ ID NO:145、SEQ ID NO:128、和SEQ ID NO:104的核苷酸序列，或与SEQ ID NO:137、SEQ ID NO:113、SEQ ID NO:145、SEQ ID NO:128、和SEQ ID NO:104具有至少80％、85％、86％、87％、88％、89％、90％、91％、92％、93％、94％、95％、96％、97％、98％或99％同一性的核苷酸序列。预期细菌可以包含一种或多种核酸，所述核酸包含SEQ ID NO:155、SEQID NO:137、SEQ ID NO:128、SEQ ID NO:148、SEQ ID NO:115、SEQ ID NO:98、和SEQ ID NO:105的核苷酸序列，或与SEQ ID NO:155、SEQ ID NO:137、SEQ ID NO:128、SEQ ID NO:148、SEQ ID NO:115、SEQ ID NO:98、和SEQ ID NO:105具有至少80％、85％、86％、87％、88％、89％、90％、91％、92％、93％、94％、95％、96％、97％、98％或99％同一性的核苷酸序列。预期细菌可以包含一种或多种核酸，所述核酸包含SEQ ID NO:155、SEQ ID NO:137、SEQ IDNO:128、SEQ ID NO:144、SEQ ID NO:115、SEQ ID NO:98、和SEQ ID NO:105的核苷酸序列，或与SEQ ID NO:155、SEQ ID NO:137、SEQ ID NO:128、SEQ ID NO:144、SEQ ID NO:115、SEQID NO:98、和SEQ ID NO:105具有至少80％、85％、86％、87％、88％、89％、90％、91％、92％、93％、94％、95％、96％、97％、98％或99％同一性的核苷酸序列。预期细菌可以包含一种或多种核酸，所述核酸包含SEQ ID NO:147、SEQ ID NO:104、SEQ ID NO:153、SEQ ID NO:97、SEQ ID NO:131、SEQ ID NO:136、和SEQ ID NO:113的核苷酸序列，或与SEQ ID NO:147、SEQID NO:104、SEQ ID NO:153、SEQ ID NO:97、SEQ ID NO:131、SEQ ID NO:136、和SEQ ID NO:113具有至少80％、85％、86％、87％、88％、89％、90％、91％、92％、93％、94％、95％、96％、97％、98％或99％同一性的核苷酸序列。预期细菌可以包含一种或多种核酸，所述核酸包含SEQ ID NO:145、SEQ ID NO:137、SEQ ID NO:153、SEQ ID NO:129、SEQ ID NO:105、SEQ IDNO:113、和SEQ ID NO:96的核苷酸序列，或与SEQ ID NO:145、SEQ ID NO:137、SEQ ID NO:153、SEQ ID NO:129、SEQ ID NO:105、SEQ ID NO:113、和SEQ ID NO:96具有至少80％、85％、86％、87％、88％、89％、90％、91％、92％、93％、94％、95％、96％、97％、98％或99％同一性的核苷酸序列。预期细菌可以包含一种或多种核酸，所述核酸包含SEQ ID NO:145、SEQID NO:137、SEQ ID NO:153、SEQ ID NO:129、SEQ ID NO:105、和SEQ ID NO:96的核苷酸序列，或与SEQ ID NO:145、SEQ ID NO:137、SEQ ID NO:153、SEQ ID NO:129、SEQ ID NO:105、和SEQ ID NO:96具有至少80％、85％、86％、87％、88％、89％、90％、91％、92％、93％、94％、95％、96％、97％、98％或99％同一性的核苷酸序列。预期细菌可以包含一种或多种核酸，所述核酸包含SEQ ID NO:139、SEQ ID NO:129、SEQ ID NO:155、SEQ ID NO:112、SEQ ID NO:99、SEQ ID NO:106、和SEQ ID NO:145的核苷酸序列，或与SEQ ID NO:139、SEQ ID NO:129、SEQ ID NO:155、SEQ ID NO:112、SEQ ID NO:99、SEQ ID NO:106、和SEQ ID NO:145具有至少80％、85％、86％、87％、88％、89％、90％、91％、92％、93％、94％、95％、96％、97％、98％或99％同一性的核苷酸序列。预期细菌可以包含一种或多种核酸，所述核酸包含SEQ IDNO:139、SEQ ID NO:154、SEQ ID NO:131、SEQ ID NO:98、SEQ ID NO:115、SEQ ID NO:106、和SEQ ID NO:149的核苷酸序列，或与SEQ ID NO:139、SEQ ID NO:154、SEQ ID NO:131、SEQID NO:98、SEQ ID NO:115、SEQ ID NO:106、和SEQ ID NO:149具有至少80％、85％、86％、87％、88％、89％、90％、91％、92％、93％、94％、95％、96％、97％、98％或99％同一性的核苷酸序列。预期细菌可以包含一种或多种核酸，所述核酸包含SEQ ID NO:137、SEQ ID NO:113、SEQ ID NO:148、SEQ ID NO:129、和SEQ ID NO:105的核苷酸序列，或与SEQ ID NO:137、SEQ ID NO:113、SEQ ID NO:148、SEQ ID NO:129、和SEQ ID NO:105具有至少80％、85％、86％、87％、88％、89％、90％、91％、92％、93％、94％、95％、96％、97％、98％或99％同一性的核苷酸序列。预期细菌可以包含一种或多种核酸，所述核酸包含SEQ ID NO:145、SEQID NO:137、SEQ ID NO:153、SEQ ID NO:129、和SEQ ID NO:105的核苷酸序列，或与SEQ IDNO:145、SEQ ID NO:137、SEQ ID NO:153、SEQ ID NO:129、和SEQ ID NO:105具有至少80％、85％、86％、87％、88％、89％、90％、91％、92％、93％、94％、95％、96％、97％、98％或99％同一性的核苷酸序列。预期细菌可以包含一种或多种核酸，所述核酸包含SEQ ID NO:155、SEQID NO:137、SEQ ID NO:128、SEQ ID NO:144、SEQ ID NO:115、SEQ ID NO:98、和SEQ ID NO:105的核苷酸序列，或与SEQ ID NO:155、SEQ ID NO:137、SEQ ID NO:128、SEQ ID NO:144、SEQ ID NO:115、SEQ ID NO:98、和SEQ ID NO:105具有至少80％、85％、86％、87％、88％、89％、90％、91％、92％、93％、94％、95％、96％、97％、98％或99％同一性的核苷酸序列。预期细菌可以包含一种或多种核酸，所述核酸包含SEQ ID NO:145、SEQ ID NO:137、SEQ IDNO:153、SEQ ID NO:129、SEQ ID NO:105、SEQ ID NO:113、和SEQ ID NO:96的核苷酸序列，或与SEQ ID NO:145、SEQ ID NO:137、SEQ ID NO:153、SEQ ID NO:129、SEQ ID NO:105、SEQID NO:113、和SEQ ID NO:96具有至少80％、85％、86％、87％、88％、89％、90％、91％、92％、93％、94％、95％、96％、97％、98％或99％同一性的核苷酸序列。预期细菌可以包含一种或多种核酸，所述核酸包含SEQ ID NO:145、SEQ ID NO:137、SEQ ID NO:153、SEQ ID NO:129、SEQ ID NO:105、和SEQ ID NO:96的核苷酸序列，或与SEQ ID NO:145、SEQ ID NO:137、SEQID NO:153、SEQ ID NO:129、SEQ ID NO:105、和SEQ ID NO:96具有至少80％、85％、86％、87％、88％、89％、90％、91％、92％、93％、94％、95％、96％、97％、98％或99％同一性的核苷酸序列。预期细菌可以包含一种或多种核酸，所述核酸包含SEQ ID NO:139、SEQ ID NO:129、SEQ ID NO:155、SEQ ID NO:112、SEQ ID NO:99、SEQ ID NO:106、和SEQ ID NO:145的核苷酸序列，或与SEQ ID NO:139、SEQ ID NO:129、SEQ ID NO:155、SEQ ID NO:112、SEQ IDNO:99、SEQ ID NO:106、和SEQ ID NO:145具有至少80％、85％、86％、87％、88％、89％、90％、91％、92％、93％、94％、95％、96％、97％、98％或99％同一性的核苷酸序列。
预期细菌可以包含一种或多种核酸，所述核酸包含SEQ ID NO:82-95中的任一个的核苷酸序列，或与SEQ ID NO:82-95中的任一个的核苷酸序列具有至少80％、85％、86％、87％、88％、89％、90％、91％、92％、93％、94％、95％、96％、97％、98％或99％同一性的核苷酸序列。
II.药物组合物/单元
本文公开的细菌可以与药学上可接受的赋形剂组合以形成药物组合物，所述药物组合物可以通过本领域已知的任何方式施用于患者。本文中使用的术语“药学上可接受的赋形剂”被理解为是指一种或多种适合于施用给受试者(例如，人受试者)的缓冲剂、载剂或赋形剂，而没有过度的毒性、刺激、变应性应答或其它问题或并发症，与合理的收益/风险比相称。赋形剂应当是“可接受的”，其含义是，与制剂的其它成分相容且对接受者无害。
药学上可接受的赋形剂包括与药物施用相容的缓冲剂、溶剂、分散介质、包衣剂、等渗剂和吸收延迟剂等。药学上可接受的赋形剂也包括填充剂、粘合剂、崩解剂、助流剂、润滑剂及其任何组合。关于赋形剂、载体、稳定剂和佐剂的其它例子，参见，例如，Handbook ofPharmaceutical Excipients,第8版,P.J.Sheskey,W.G.Cook,和C.G.Cable编,Pharmaceutical Press,伦敦,UK[2017]。此类介质和试剂用于药学活性物质的用途是本领域已知的。
所涵盖的细菌可以用在呈本领域技术人员已知的任何形式(例如稳定形式)的所公开的组合物中，包括以低压冻干状态(任选地使用一种或多种适当的冷冻保护剂)、冷冻(例如，在标准的或过冷的冰柜中)、喷雾干燥和/或冷冻干燥。“稳定的”制剂或组合物是这样的制剂或组合物：其中的生物活性物质在储存后基本上保持其物理稳定性、化学稳定性和/或生物活性。可以在选定的温度和湿度条件下测量稳定性选定的时间段。趋势分析可以用于估计物质在实际储存该时间段之前的预期贮存期限。例如，对于活细菌，稳定性可以定义为在预定的温度、湿度和时间段条件下失去1个对数的cfu/g干燥制剂所需的时间。
本文公开的细菌可以与一种或多种冷冻保护剂组合。示例性的冷冻保护剂包括低聚果糖(例如，
)、海藻糖、麦芽糊精、海藻酸钠、脯氨酸、谷氨酸、甘氨酸(例如，甘氨酸甜菜碱)、单糖、二糖或多糖(诸如葡萄糖、蔗糖、麦芽糖、乳糖)、多元醇(诸如甘露醇、山梨醇或甘油)、葡聚糖、DMSO、甲基纤维素、丙二醇、聚乙烯吡咯烷酮、非离子型表面活性剂诸如吐温80和/或它们的任意组合。
药物组合物应当配制成与其预期的施用途径相容。本文公开的所涵盖的细菌组合物可以通过任何合适的方法制备，并且可以配制成多种形式并通过多种不同方式施用。所涵盖的组合物可以在包含所需的常规可接受的载剂、佐剂和媒介物的制剂中口服地、直肠地或肠内地施用。本文中使用的“直肠施用”理解为包括通过灌肠剂、栓剂或结肠镜检查的施用。所公开的药物组合物可以例如适用于推注施用或推注释放。在一个示例性实施方案中，口服施用公开的细菌组合物。
用于口服施用的固体剂型包括胶囊剂、片剂(tablets)、囊片、丸剂、药片(troches)、锭剂、粉剂和颗粒。胶囊通常包含含有细菌组合物的核心物质和包封核心物质的壳壁。在某些实施方案中，核心物质包含固体、液体和乳剂中的至少一种。在某些实施方案中，壳壁物质包含软明胶、硬明胶和聚合物中的至少一种。合适的聚合物包括、但不限于：纤维素聚合物诸如羟丙基纤维素、羟乙基纤维素、羟丙基甲基纤维素(HPMC)、甲基纤维素、乙基纤维素、乙酸纤维素、邻苯二甲酸乙酸纤维素、偏苯三甲酸乙酸纤维素、邻苯二甲酸羟丙基甲基纤维素、琥珀酸羟丙基甲基纤维素和羧甲纤维素钠；丙烯酸聚合物和共聚物，诸如由丙烯酸、甲基丙烯酸、丙烯酸甲酯、甲基丙烯酸铵、丙烯酸乙酯、甲基丙烯酸甲酯和/或甲基丙烯酸乙酯形成的那些(例如，在商业名称“
”下销售的那些共聚物)；乙烯基聚合物和共聚物诸如聚乙烯吡咯烷酮、聚乙酸乙烯酯、聚乙酸乙烯酯邻苯二甲酸酯、乙酸乙烯酯巴豆酸共聚物和乙烯-乙酸乙烯酯共聚物；和紫胶(纯化的紫胶)。在某些实施方案中，至少一种聚合物用作掩味剂。
片剂、丸剂等可以被压制、多次压制、多次分层和/或包衣。所涵盖的包衣可以是单层或多层。在一个实施方案中，所涵盖的包衣材料包含从植物、真菌和微生物中的至少一种中提取的糖、多糖和糖蛋白中的至少一种。非限制性例子包括玉米淀粉、小麦淀粉、马铃薯淀粉、木薯淀粉、纤维素、半纤维素、葡聚糖、麦芽糊精、环糊精、菊糖、果胶、甘露聚糖、阿拉伯树胶、槐豆胶、甜荚豆胶、瓜尔胶、刺梧桐树胶、印度树胶(gum ghatti)、黄蓍胶、funori、角叉菜胶、紫菜多糖、琼脂、海藻酸盐、壳聚糖或结冷胶。在某些实施方案中，所涵盖的包衣材料包含蛋白。在某些实施方案中，所涵盖的包衣材料包含脂肪和油中的至少一种。在某些实施方案中，脂肪和油中的至少一种是高温熔化的。在某些实施方案中，脂肪和油中的至少一种被氢化或部分氢化。在某些实施方案中，脂肪和油中的至少一种源自植物。在某些实施方案中，脂肪和油中的至少一种包含甘油酯、游离脂肪酸和脂肪酸酯中的至少一种。在某些实施方案中，所涵盖的包衣材料包含至少一种可食用蜡。所涵盖的可食用蜡可源自动物、昆虫或植物。非限制性例子包括蜂蜡、羊毛脂、杨梅蜡、巴西棕榈蜡和米糠蜡。片剂和丸剂另外可以用肠溶包衣或反向肠溶包衣来制备。
备选地，可以将包含本文公开的细菌组合物的粉末或颗粒掺入食物产品中。在某些实施方案中，所涵盖的食物产品是用于口服施用的饮料。合适的饮料的非限制性实例包括水、果汁、水果饮料、人工调味的饮料、人工增甜的饮料、碳酸饮料、运动饮料、液体乳制品、奶昔、酒精饮料、含咖啡因的饮料、婴儿配方等。其它合适的口服施用方式包括水性和非水性溶液、乳剂、混悬液和溶液和/或从非泡腾颗粒重构的混悬液，其含有合适的溶剂、防腐剂、乳化剂、助悬剂、稀释剂、甜味剂、着色剂和矫味剂中的至少一种。
含有本文公开的细菌的药物组合物可以以单位剂型(即药物单位)存在。组合物，例如，本文提供的药物单位，可以包括任何合适量的细菌，其通过细菌的总质量或菌落形成单位来测量。
例如，公开的药物组合物或单位可以包括约103cfu至约1012cfu、约106cfu至约1012cfu、约107cfu至约1012cfu、约108cfu至约1012cfu、约109cfu至约1012cfu、约1010cfu至约1012cfu、约1011cfu至约1012cfu、约103cfu至约1011cfu、约106cfu至约1011cfu、约107cfu至约1011cfu、约108cfu至约1011cfu、约109cfu至约1011cfu、约1010cfu至约1011cfu、约103cfu至约1010cfu、约106cfu至约1010cfu、约107cfu至约1010cfu、约108cfu至约1010cfu、约109cfu至约1010cfu、约103cfu至约109cfu、约106cfu至约109cfu、约107cfu至约109cfu、约108cfu至约109cfu、约103cfu至约108cfu、约106cfu至约108cfu、约107cfu至约108cfu、约103cfu至约107cfu、约106cfu至约107cfu、或约103cfu至约106cfu的每种细菌菌株，或可以包括约103cfu、约106cfu、约107cfu、约108cfu、约109cfu、约1010cfu、约1011cfu或约1012cfu的细菌。
III.治疗用途
本文中公开的组合物和方法可以用于治疗受试者中与草酸盐量升高相关的各种疾病或病症。本文中使用的“受试者中草酸盐量升高”可以表示相对于没有疾病或病症的受试者，受试者的体液(例如，血液、血浆、血清或尿)、组织和/或细胞中的草酸盐量升高。本公开内容提供了治疗受试者中与草酸盐量升高相关的疾病或病症的方法。所涵盖的方法包括单独地或与另一种治疗剂组合地给所述受试者施用有效量的本文中公开的细菌或药物组合物，以治疗受试者中与草酸盐量升高相关的疾病或病症。
与草酸盐量升高相关的疾病或病症的一个例子是高草酸尿症，例如，原发性、肠源性、饮食性或特发性高草酸尿症。高草酸尿症或增加的尿草酸盐水平可以特征在于每天大于40mg(大约440μmol)或30mg的人体尿草酸盐排泄。示例性的临床截止水平是男性43mg/天(大约475μmol)和女性32mg/天(大约350μmol)。高草酸尿症也可定义为每天每克尿肌酸酐大于30mg的尿草酸盐排泄。轻度高草酸尿症患者每天可排泄至少30-60(342-684μmol)或40-60(456-684μmol)mg草酸盐。肠源性高草酸尿症患者每天可排泄至少80mg尿草酸盐(912μmol)，且原发性高草酸尿症患者每天可排泄至少200mg(2280μmol)。
升高的尿草酸盐已经与肾脏的许多健康问题关联，例如肾结石(例如，复发性草酸钙肾结石)、肾钙质沉着、肾石病(nephrolithiasis)、肾损伤或终末期肾病。草酸钙还可能沉积在泌尿道、结肠、小肠、眼、血管、关节、骨、肌肉、心脏和其它主要器官中，对它们造成损害。因此，与草酸盐量升高相关的其它示例性疾病或病症包括肾病症(例如，肾结石、肾钙质沉着、多囊性肾病、肾石病或肾衰竭(包括进行性、慢性或终末期肾衰竭))、泌尿道病症(例如，特发性尿路结石病或尿石病)、胃肠道病症(例如，炎性肠病、克罗恩氏病或溃疡性结肠炎)、胰腺病症(例如，外分泌胰腺功能不全)、关节病症、眼病、肝病症、乙二醇中毒、囊性纤维化、脂肪痢、回肠疾病、外阴痛、心脏传导病症、骨质疏松症以及由胃旁路或其它类型的胃肠手术引起的并发症。
本文中使用的“治疗”是指治疗受试者(例如人)的疾病。这包括：(a)抑制疾病，即阻止其发展；和(b)缓解疾病，即造成疾病状态的消退。本文中使用的术语”受试者”和“患者”表示将用本文所述的方法和组合物治疗的生物体。这样的生物体优选地包括、但不限于哺乳动物，例如，人、伴侣动物(例如，狗、猫或兔)或家畜动物(例如，母牛、绵羊、猪、山羊、马、驴和骡子、水牛、公牛或骆驼))。
应当理解，药物组合物或细菌的确切剂量由个体医师考虑到要治疗的患者进行选择，一般而言，调整剂量和施用以向正在治疗的患者提供有效量的细菌剂。本文中使用的“有效量”表示引发有益的或期望的生物反应所需的量。有效量可以在一次或多次施用、应用或剂量中施用，并且不意图限于特定制剂或施用途径。本领域普通技术人员会理解，药物单元、药物组合物或细菌菌株的有效量可以随诸如以下因素变化：期望的生物学终点、待递送的药物、靶组织、施用途径等。可以考虑的其它因素包括疾病状态的严重程度；正在治疗的患者的年龄、体重和性别；饮食、施用的时间和频率；药物组合；反应敏感性；和对疗法的耐受性/应答。
所涵盖的方法可以进一步包括向受试者施用特殊营养物以支持细菌的定殖。示例性的特殊营养物包括海洋多糖，例如，紫菜多糖。例如，可以在公开的细菌之前、同时或之后将公开的特殊营养物施用给受试者。
相对于未治疗或对照受试者中的草酸盐水平，本文所述的方法和组合物可以将受试者中例如受试者的体液(例如，血液、血浆、血清或尿)、组织和/或细胞中的草酸盐水平降低至少约10％、约20％、约30％、约40％、约50％、约60％、约70％、约80％、约90％、约95％或更多。
所涵盖的方法可以包括每12小时、24小时、1天、2天、3天、4天、5天、6天、7天、1周、2周、3周、4周、1个月、2个月、3个月、4个月、5个月或6个月给受试者施用公开的细菌或药物组合物。在某些实施方案中，公开的细菌或药物组合物向受试者的连续施用之间的时间大于12小时、24小时、36小时、48小时、2天、3天、4天、5天、6天、7天、1周、2周、3周或4周。
在某些实施方案中，以相同频率向受试者施用公开的细菌和公开的特殊营养物(例如，海洋多糖，例如，紫菜多糖)。例如，可以每12小时、24小时、1天、2天、3天、4天、5天、6天、7天、1周、2周、3周、4周、1个月、2个月、3个月、4个月、5个月或6个月向受试者施用所述细菌和所述特殊营养物。在某些实施方案中，以不同频率向受试者施用公开的细菌和公开的特殊营养物(例如，海洋多糖，例如，紫菜多糖)。例如，可以每12小时、24小时、1天、2天、3天、4天、5天、6天、7天、1周、2周、3周、4周、1个月、2个月、3个月、4个月、5个月或6个月向受试者施用细菌，并可以每12小时、24小时、1天、2天、3天、4天、5天、6天、7天、1周、2周、3周、4周、1个月、2个月、3个月、4个月、5个月或6个月向受试者施用特殊营养物。例如，在某些实施方案中，可以每1周、2周、3周、4周、1个月、2个月、3个月、4个月、5个月或6个月向受试者施用细菌，并可以每12小时、24小时、1天、2天、3天、4天、5天、6天或7天向受试者施用特殊营养物。
本文所述的方法和组合物可以单独使用，或与其它治疗剂和/或模态联合使用。本文所用的术语“联合”施用被理解为是指，在受试者患有病症的过程中向受试者递送两种(或更多种)不同的治疗，使得所述治疗对患者的影响在某个时间点重叠。在某些实施方案中，当第二种治疗的递送开始时，一种治疗的递送仍在发生，从而在施用方面存在重叠。这有时在本文中被称为“同时”或“并行递送”。在其它实施方案中，一种治疗的递送在另一种治疗的递送开始之前结束。在任一种情况的某些实施方案中，由于联合施用，治疗更有效。例如，第二种治疗更有效，例如，用更少的第二种治疗观察到等同效果，或与在没有第一种治疗存在下施用第二种治疗所观察到的效果相比，第二种治疗在更大程度上减轻症状，或用第一种治疗观察到类似的情形。在某些实施方案中，递送使得症状或与病症相关的其它参数的减轻大于在没有另一种治疗存在下递送的一种治疗所观察到的减轻。两种治疗的效果可以是部分累加的、完全累加的或大于累加的。递送可以使得递送的第一种治疗的效果在递送第二种治疗时仍然是可检测到的。在某些实施方案中，第一种和/或第二种治疗的副作用由于联合施用而减少。
在某些实施方案中，本文描述的方法或组合物与一种或多种额外疗法联合施用。在某些实施方案中，所涵盖的额外疗法可以包括：高液体摄入；低盐、低草酸盐和/或高钙饮食；柠檬酸盐、钙、正磷酸盐和/或吡多辛/维生素B6补充；草酸脱羧酶；和/或刺激结肠分泌草酸盐的肽。
贯穿本说明书，在组合物被描述为具有、包括或包含特定组分的情况下，或者在过程和方法被描述为具有、包括或包含特定步骤的情况下，预期另外存在这样的本公开内容的组合物，其基本上由所述组分组成或由所述组分组成，且存在这样的根据本公开内容的过程和方法，其基本上由所述处理步骤组成或由所述处理步骤组成。
在本申请中，在一个要素或组分据称被包括在和/或选自所述要素或组分的列表中的情况下，应当理解，该要素或组分可以是所述要素或组分中的任一种，或者所述要素或组分可以选自由所述要素或组分中的两种或更多种组成的组。
进一步地，应当理解，本文描述的组合物或方法的要素和/或特征可以以多种方式组合而不背离本公开内容的精神和范围，无论在本文中明示还是暗示。例如，在提及具体化合物的情况下，该化合物可以用在本公开内容的组合物的不同实施方案中和/或本公开内容的方法中，除非从上下文另有理解。换而言之，在本申请中，已经以使申请的撰写和描述清楚和简明的方式描述和描绘实施方案，但是意图并且将理解，可以在不脱离本教导和公开的情况下以各种方式组合或分离实施方案。例如，应当理解，本文描述和描绘的所有特征可以适用于本文描述和描绘的本公开内容的所有方面。
应当理解，表述“至少一个”包括在该表述之后的单独每个描述的对象和所描述的对象中的两个或更多个的各种组合，除非从上下文和应用另有理解。关于三个或更多个描述的对象的表述“和/或”应理解为具有相同含义，除非从上下文另有理解。
术语“包括”、“包含”、“具有”、“拥有”、“含有”，包括其语法等效词，一般应当理解为开放式的和非限制性的，例如，不排除额外的未描述的要素或步骤，除非从上下文另有特别描述或理解。
当术语“约”的使用是在数量值之前时，本公开内容还包括具体的数量值本身，除非另外特别说明。本文中使用的术语“约”表示从标称值±10％的变化，除非另外指出或推断。
应当理解，只要本公开内容保持可操作，步骤的顺序或执行某些动作的顺序是不重要的。此外，可以同时进行两个或更多个步骤或动作。
任何和所有实例或示例性语言在本文中的使用，例如“诸如”或“包括”，仅意图更好地举例说明本公开内容，而不对本公开内容的范围造成限制，除非声明。在说明书中的语言不应解释为指出任何未声明的要素是本公开内容的实践所必需的。
实施例
以下实施例仅是举例说明性的，且无意以任何方式限制本公开内容的范围或内容。
实施例1-降解草酸盐的拟杆菌的构建
通过添加来自其它生物体的转基因(例如SEQ ID NO:1-31)，可以实现向草酸盐原初属(oxalate-
genus)拟杆菌属的成员添加草酸盐降解能力。例如，包括用于表达OxlT、OXS和OXC的表达盒的构建体可用于实现拟杆菌属中的草酸盐降解。示例性的OxIT编码序列描述在SEQ ID NO:17-21中，示例性的OXS编码序列描述在SEQ ID NO:14-16中，且示例性的OXC编码序列描述在SEQ ID NO:22-26中。所涵盖的构建体还可以包括用于表达FCOCT、ACOCT或SCOCT的表达盒。示例性的FCOCT编码序列描述在SEQ ID NO:4-8中，示例性的ACOCT编码序列描述在SEQ ID NO:1-2中，且示例性的SCOCT编码序列描述在SEQ ID NO:28-31中。OXS和/或OXC的额外拷贝的表达也可以改善草酸盐降解。
图3显示了不同的质粒(SEQ ID NO:63-67)，它们可以单独使用或组合使用以产生具有草酸盐降解能力的拟杆菌。pZR748(SEQ ID NO:63)包括酿酒酵母OXS、酿酒酵母OXC和产甲酸草酸杆菌OxIT的编码序列。pZR753(SEQ ID NO:64)包括大肠杆菌FCOCT、产甲酸草酸杆菌OXC、酿酒酵母OXS和产甲酸草酸杆菌OxIT的编码序列。pZR761(SEQ ID NO:65)包括酿酒酵母OXS和酿酒酵母OXC的编码序列。pZR856(SEQ ID NO:66)包括产甲酸草酸杆菌OxIT的编码序列。pZR878(SEQ ID NO:67)包括大肠杆菌FCOCT、产甲酸草酸杆菌OXC、酿酒酵母OXS、酿酒酵母OXC、拟南芥OXS和大肠杆菌ACOCT的编码序列。
每个拷贝的草酸盐分解代谢相关基因可操作地连接至一个拷贝的噬菌体衍生的启动子P_BfP1E6(SEQ ID NO:68)或其弱化版本P_BfP4E5(SEQ ID NO:69)。也可以使用其它启动子，包括P_BfP2E5(SEQ ID NO:80)和与噬菌体衍生的共有序列(SEQ ID NO:70)匹配的其它启动子。为了优化酶的翻译速率，将酶融合到与高强度核糖体结合位点(RBS)(SEQ IDNO:78)偶联的前导肽或与高强度RBS(SEQ ID NO:79)偶联的NanoLuc萤光素酶报告酶。也可以使用其它核糖体结合位点和翻译融合体。质粒携带NBU2整合酶基因，该基因催化质粒在拟杆菌基因组中丝氨酸-tRNA基因的3'端的基因组整合。
一旦构建，就将质粒转化到带有RK2接合质粒的大肠杆菌S17-1供体细胞中。RK2接合机制允许将带有RP4转移起点的质粒接合转移到各种物种，包括拟杆菌。将这些细胞与拟杆菌接受者细胞混合并铺板到非选择性BHI琼脂平板上在37℃温育过夜。然后，刮平板并将大肠杆菌S17-1和拟杆菌的混合物转移到含有庆大霉素的选择性培养基中以抑制大肠杆菌并在厌氧条件下培养。多种选择性标志物(诸如四环素、红霉素或氯霉素)可以用于将多种构建体递送到单个拟杆菌属菌株中。
实施例2-液体培养物中草酸盐降解的体外试验
在体外测定中关于草酸盐降解能力测定了如实施例1中所述构建的经工程改造的拟杆菌。将拟杆菌细胞从过夜培养物以1:10稀释到BHIS液体培养基中，其中添加了10或30mM的草酸钠。将培养物在37℃厌氧温育过夜。48小时后，将培养物离心以分离出细胞，并取上清液进行分析。使用595nm草酸氧化酶试剂盒(Sigma-Aldrich,目录号MAK315-1KT)分析样品，并与标准曲线对比以计算草酸盐消耗速率。在普通拟杆菌ATCC 8482的背景下来自试验质粒pZR748(SEQ ID NO:63)、pZR753(SEQ ID NO:64)、pZR753+pZR761(SEQ ID NO:64、65)和pZR856+pZR878(SEQ ID NO:66、67)的所得数据显示在图4中。如所示，每个经工程改造的菌株的草酸盐消耗相对于野生型有所增加，所述野生型没有显示草酸盐消耗能力。
实施例3-将特殊营养物消耗工程化到拟杆菌中
多糖利用基因座(PUL)是赋予细菌消耗新碳水化合物的能力的移动遗传元件。在消耗紫菜多糖的拟杆菌属菌株分离株NB001(SEQ ID NO:72)中鉴定出紫菜多糖消耗PUL，并将PUL的60kb区域克隆进细菌人工染色体(BAC)中以制备pWD035(SEQ ID NO:73)。pWD035的内含物如图5所示。
使用大肠杆菌S17-1将质粒pWD035缀合到普通拟杆菌ATCC 8482中以产生菌株NB075。测试了该菌株的消耗海洋多糖紫菜多糖的能力。将来自过夜培养物的细胞以1:50稀释到含有0.2％的来自条斑紫菜(Porphyra yezoensis)紫菜的紫菜多糖提取物的Salyer氏基本培养基中。在37℃厌氧温育超过14小时，使用平板读数器测量代表细胞生长的OD600。图6中显示的结果证实，只有接受紫菜多糖PUL的细胞才能获得消耗紫菜多糖进行生长的能力。
实施例4-可以消耗草酸盐和特殊营养物的工程菌株
图7显示了可以单独或组合使用以产生具有高效草酸盐降解能力的拟杆菌的质粒(SEQ ID NO:75-77)。pZR0879(SEQ ID NO:75)包括大肠杆菌FCOCT、产甲酸草酸杆菌OXC、酿酒酵母OXS、酿酒酵母OXC、大肠杆菌ACOCT和产甲酸草酸杆菌OxIT的编码序列。pZR0901(SEQID NO:76)包括大肠杆菌FCOCT、产甲酸草酸杆菌OXC、酿酒酵母OXS、酿酒酵母OXC、拟南芥OXS、大肠杆菌ACOCT和产甲酸草酸杆菌OxIT的编码序列。pZR1871(SEQ ID NO:77)包括大肠杆菌FCOCT、产甲酸草酸杆菌OXC、酿酒酵母OXS、酿酒酵母OXC、拟南芥OXS、大肠杆菌ACOCT和产甲酸草酸杆菌OxIT的编码序列。
通过将质粒pZR0879、pZR0901和pZR1871向菌株NB075中的缀合和选择性铺板(在实施例3中描述)，产生能够同时消耗紫菜多糖和草酸盐的菌株。还包括空载体(pZR1622,SEQ ID 74)以构建不消耗草酸盐的对照菌株。对于缀合体中的两个(pZR879和pZR1871)，为了找到具有增加的草酸盐消耗速率的菌株，将约3,000个菌落从单个选择性平板分离。然后对这些克隆分离株进行实施例1中描述的草酸盐消耗测定的改进版本，其在Labcyte Echo525声学液体处理机器人上以高通量运行。大约0.001％的克隆分离株被证明是离群值，表明比大多数分离株明显更快的草酸盐消耗速率。在Illumina iSeq 100上的全基因组测序被用于确认这些菌株在其基因组中携带目标质粒的两个拷贝，可能导致草酸盐途径基因的表达增加。产生的菌株的详细信息，包括质粒拷贝数，描述在表1中。
表1–被工程改造成消耗紫菜多糖和草酸盐两者的拟杆菌属菌株
在体外草酸盐消耗测定中测试了四种消耗草酸盐的菌株(sWW152、NB120、sWW554和sWW626)以对比它们各自的草酸盐消耗速率。将每种菌株在BHIS培养基中在37℃厌氧生长至饱和。然后将培养物沉淀并用补充有4mM草酸盐的新鲜BHIS重新悬浮在起始体积的十分之一中。10倍浓缩培养物的稀释铺板指示，它们含有约1x1010cfu/ml，这是这些菌株在肠道定殖时在盲肠中达到的近似丰度。将浓缩的培养物转移回厌氧室，并在37℃温育。以规律的时间间隔，从厌氧室中取出培养物等分试样，转移到冰上，沉淀，并冷冻上清液。然后测量上清液等分试样以确定剩余草酸盐的浓度。结果描述在图8中。正如预期的那样，每种菌株都降低了上清液中的草酸盐浓度，其中sWW626在15分钟内消耗了75％的草酸盐。
实施例5-大鼠盲肠内容物中草酸盐消耗速率的离体试验
接下来对在实施例4中描述的消耗草酸盐/紫菜多糖的候选菌株进行离体草酸盐消耗测定，该测定设计成尽可能接近地模拟体内条件。首先，收获喂食低草酸盐饮食的多只Sprague-Dawley大鼠的盲肠，将内容物挤出并3倍稀释到蒸馏水中以帮助移液。加入草酸盐至4mM的终浓度。然后添加消耗草酸盐的菌株至1x1010cfu/ml的终浓度以匹配它们在盲肠中的预期体内丰度。如上所述监测随时间的草酸盐消耗。结果描述在图9中。再次，每种菌株消耗盲肠内容物中的草酸盐，尽管盲肠内容物中的草酸盐消耗速率比在BHIS培养基中慢约10倍。
实施例6-在继发性高草酸尿症的大鼠模型中的体内效力
为了证明消耗草酸盐的拟杆菌属菌株可以用于治疗高草酸尿症，在继发性高草酸尿症的大鼠模型中对候选菌株进行了体内试验。高草酸尿症通过低钙饮食引起，所述低钙饮食导致饮食草酸盐的过度吸收。图10和图11描绘了分别设计成测试候选治疗菌株sWW152和sWW554的效力的体内实验的结果。将重约200克的雄性Sprague-Dawley大鼠单独圈养在代谢笼(Tecniplast,3700M022)中并喂食低钙(<0.01％)、低草酸盐基础饮食(Envigo,TD.160869)。每天收集24小时尿并在每个实验中分析总草酸盐。从第3天开始并在实验的剩余时间里继续，以新鲜菠菜的形式向基础饮食中每天添加52μmol草酸盐(图10)或每天添加30μmol草酸盐(图11)，导致高草酸尿症。从第2天(图10)或第7天(图11)开始并在实验的剩余时间里继续，还将780mg紫菜多糖/天添加到基础饮食中。在第7天，通过向饮用水中添加109cfu来施用不能降解草酸盐的对照菌株(图10中的NB075或图11中的NB124)或治疗性草酸盐降解菌株(图10中的sWW152或图11中的sWW554)。与对照菌株相比，sWW152的添加导致尿草酸盐的32％减少(图10,p＝0.005)，而改良菌株sWW554的添加导致与对照菌株相比尿草酸盐的46％减少(图11,p＝0.0003)。在有紫菜多糖存在下，对照和治疗菌株均以高丰度定殖于大鼠肠道，在几天内形成大约3*109cfu/mL粪便(图12)。这些结果证明了经工程改造的草酸盐降解拟杆菌在继发性高草酸尿症的啮齿动物模型中降低尿草酸盐的能力。此外，鉴于菌株sWW554具有比sWW152更大的治疗影响，增加菌株的草酸盐消耗速率似乎转化为提高的体内效力。
实施例7-在继发性高草酸尿症的大鼠模型中的体内效力
为了证明消耗草酸盐的拟杆菌属菌株可以用于治疗高草酸尿症，在继发性高草酸尿症的大鼠模型中对候选菌株进行了体内试验。
将sWW626(如实施例4中所述)修饰为含有红霉素抗性盒以制备菌株sWW627。图13A和图13B描绘了设计成测试候选治疗菌株sWW627的效力的体内实验的结果。
将重约600克的雄性Sprague-Dawley大鼠单独圈养在代谢笼(Tecniplast,3701M081)中，并饲喂每只大鼠每天含有86μmol草酸盐的高脂肪(40％)基础饮食。每天收集24小时尿并在每个实验中分析总草酸盐。在第1-2天测量基线尿草酸盐。从第3天开始并在实验的剩余时间里继续，将菠菜添加到基础饮食中，导致高草酸尿症。在第7天，给大鼠接种无菌株、能够在紫菜多糖上生长但缺乏草酸盐消耗基因的对照菌株(NB124)、或能够在紫菜多糖上生长并消耗草酸盐的治疗菌株(sWW627)。通过向饮用水中添加109cfu来施用菌株。从第12天开始，将紫菜多糖添加到饮用水中(并在整个实验过程中增加，如在图13A和图13B中所示)。
通过计算每克粪便的菌落形成单位(CFU)来测量的菌株丰度显示在图13A中。如所示，菌株丰度的增加与饮食中较高的紫菜多糖浓度相对应。尿草酸盐显示在图13B中。如所示，相对于无菌株或对照菌株，菌株sWW627的施用减少了尿草酸盐。随着饮食中紫菜多糖浓度增加，sWW627减少的尿草酸盐增加。
实施例8-在继发性高草酸尿症的大鼠模型中的体内效力
为了证明消耗草酸盐的拟杆菌属菌株可以用于治疗高草酸尿症，在继发性高草酸尿症的大鼠模型中对候选菌株进行了体内试验。
通过Route-en-Y胃旁路(RYGB)手术在大鼠中诱导高草酸尿症。给大鼠喂食包括紫菜多糖、钙和菠菜的饮食。收集每只动物的尿并每天测量体积和草酸盐浓度。通过EnzyChrom草酸盐测定试剂盒(EnzyChrom Oxalate Assay Kit，BioAssay Systems,Hayward,CA)测量草酸盐浓度。给大鼠施用能够在紫菜多糖上生长并消耗草酸盐的治疗菌株(sWW627，在实施例7中描述)或施用对照菌株。
结果描述在图14中。接受RYGB手术并管饲对照菌株的大鼠显示出比接受假手术的大鼠更高水平的尿草酸盐。接受RYGB手术并施用治疗菌株(sWW627)的大鼠显示出相对于施用对照菌株的大鼠减少的尿草酸盐，并逆转了RYGB手术的影响。
实施例8-核糖体结合位点(RBS)的工程设计
从合成文库中筛选核糖体结合位点(RBS)以产生一组RBS序列，所述序列可用于调节草酸盐降解基因的表达。对于每个感兴趣的草酸盐消耗基因，从文库中筛选了超过1000个RBS序列，其中简并序列NNNNNNNNWWWAAAWWTWANAAA位于每个基因的翻译起始位点的上游紧邻处。每个基因也在C端与萤光素酶蛋白翻译融合。在每个RBS的上游，转录由P_BfP1E6启动子(SEQ ID NO:68)驱动。将含有表达盒的质粒整合在普通拟杆菌基因组中，且独特的RBS的单个克隆在BHIS培养基中生长至饱和。用Nano-Glo萤光素酶测定系统(PromegaCorporation,Madison,WI)测量每种饱和培养物的发光。
图15A描绘了每个指定基因的每个克隆的发光值。将选定的克隆分离并测序以确定每个感兴趣的RBS序列。SEQ ID NO:96-103描述了具有八个感兴趣的RBS的拟南芥OXS编码序列。在SEQ ID NO:96-103中的RBS分别被称作RBS1-8。SEQ ID NO:104-111描述了具有八个感兴趣的RBS的酿酒酵母OXS编码序列。在SEQ ID NO:104-111中的RBS分别被称作RBS1-8。SEQ ID NO:112-119描述了具有八个感兴趣的RBS的产甲酸草酸杆菌OXC编码序列。在SEQ ID NO:112-119中的RBS分别被称作RBS1-8。SEQ ID NO:120-127描述了具有八个感兴趣的RBS的酿酒酵母OXC编码序列。在SEQ ID NO:120-127中的RBS分别被称作RBS1-8。SEQID NO:128-135描述了具有八个感兴趣的RBS的大肠杆菌ACOCT编码序列。在SEQ ID NO:128-135中的RBS分别被称作RBS1-8。SEQ ID NO:136-143描述了具有八个感兴趣的RBS的大肠杆菌FCOCT编码序列。在SEQ ID NO:136-143中的RBS分别被称作RBS1-8。SEQ ID NO:144-151描述了具有八个感兴趣的RBS的产甲酸草酸杆菌OxIT编码序列。在SEQ ID NO:144-151中的RBS分别被称作RBS1-8。SEQ ID NO:152-162描述了具有11个感兴趣的RBS的密码子优化的酿酒酵母OXC编码序列。在SEQ ID NO:152-162中的RBS分别被称作RBS1-8、RBS1b、RBS2b和RBS3b。
用上述序列重构质粒并整合进普通拟杆菌的基因组中。如上所述测定培养物的发光。结果显示在图15B中。
实施例9-在液体培养物中草酸盐降解的体外试验
在体外测定中测定了经工程改造的拟杆菌的草酸盐降解能力。
将拟杆菌细胞从过夜培养物中以1:10稀释到BHIS液体培养基中，其中添加了16mM草酸盐。将培养物在37℃厌氧温育。20小时后，将培养物离心以分离出细胞，并取上清液进行分析。使用595nm草酸氧化酶试剂盒(Sigma-Aldrich,目录号MAK315-1KT)分析样品并与标准曲线进行对比以计算草酸盐消耗速率。
试验的菌株包括NB136(不含引入的草酸盐消耗基因)、sWW152(如实施例4中所述)、sWW627(如实施例7中所述)和sWW1077。SWW1077包含SEQ ID NO:82，其包括经工程改造的草酸盐消耗途径以及在实施例8中鉴定的经工程改造的RBS(包括RBS2-大肠杆菌FCOCT(SEQ ID NO:137)、RBS2-产甲酸草酸杆菌OXC(SEQ ID NO:113)、RBS2-产甲酸草酸杆菌OxIT(SEQ ID NO:145)、RBS1-大肠杆菌ACOCT(SEQ ID NO:128)和RBS1-酿酒酵母OXS(SEQ IDNO:104))。结果显示在图16中。如图所示，每种经工程改造的菌株的草酸盐消耗相对于对照菌株增加，且sWW1077的草酸盐消耗最大。
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序列表
SEQ ID NO:1(来自醋化醋杆菌菌株1023的乙酰辅酶A:草酸辅酶A转移酶(ACOCT)；EC 2.8.3.19；UniProt Accession A9X6P9；Aa_CoAT3)：
ATGACTGAAACCACGCCAGCAAAACCGAAGGGGCCCTTTGATGGGCTCCTCGTTATTGATCTGACACATGTGCTGAATGGTCCGTTCGGCACAACAATTCTAACAGATCTGGGGGCTCGTACAATCAAGATCGAGCCACCGGGGCATGGGGATGATACGCGTACCTATGGCCCATATGTTGGAGATCAATCGCTTTACTTCTCTTTTGTAAACAGGGGAAAGGAAAGCATTGTCCTTAACCTCAAGGATGAGGGCGATCGAGCGATTTTTCTGGAAATGGTGCGTAAGGCCGATGTGCTGGCGGAAAACTTCCGCCCCGGCGTGATGGACCGTTTGGGTTTCAATTATGAGGAACTGGCAAAGATCAATCCACGGTTGATTTATGCATCTTCATCAGGGTTCGGTCAGACAGGCCCGCTCGCACATTATCCTGCCTACGATACTATTGTGCAGGCCATGAGCGGCATCATGATGGCCACGGGCTTTCCCGATGGTCCGCCAACGCGGGTAGGGGGCACATCGCTTTCTGATCTGTGCGGTGGGGTTTTCATGTTCTGCGGCATTGCCAGTGCGCTTTATGCGCGTGAGCGCACAGGGAAAGGGGCACACATTGATGTTTCAATGTTTGATGGTACCTTGGCATTTTTGCAGCATGCTCTGATGTGCTGGTCTGCTACAGGCAAAGCCCCTGCGCGTATTGGCAATCGCCATCCTTATATGGCGCCTTTTGATGTGTTTCAGGCGCAGGACAAGCCTTTTGTCATCTGTTGTGGGAATGACCATCTTTTTAAGGCGCTTTGCGATGTGATTGGTGCGCCTGAACTGGCGACAGACCCGCGTTTTGTCGAAAATCATGATAGAATGGCCAATAATGATGCGCTGAAAGCTGCATTGGAAAAAGCACTTTCCAAACAGCCAGCGGCGCATTGGCTGGATGTTATTCACAAAGCCGGTGTGCCTGTTGGGCCGTTGCTGGACGTGGCAGAGGCGGCAAATCTGCCACAAACAGCCGCGCGGAATATGCTGATTAAATCTGGCGGGGTGATGATGCCCGGTAACCCGGTGAAAATCAGTGGGTATGATGACCCACATGAACGGCCCGGCGCCCCTAAGCTGGATGAGCAGGGCGCGGCGTTGCGTAAGGAATTTGCCGCACCAGAGGCAAAATGA
SEQ ID NO:2(来自大肠杆菌MG1655的乙酰辅酶A:草酸辅酶A转移酶(ACOCT)；EC2.8.3.19；UniProt Accession P76518；Ec_yfdE)：
ATGACAAATAATGAAAGCAAAGGGCCGTTTGAAGGCTTATTAGTTATCGATATGACACATGTCCTTAATGGACCTTTCGGAACTCAACTTCTTTGTAATATGGGCGCAAGGGTAATTAAAGTTGAGCCGCCGGGTCATGGTGATGATACCCGCACATTTGGTCCCTATGTGGATGGACAGTCACTCTATTACAGTTTTATTAATCATGGCAAAGAGAGTGTGGTTCTTGATTTAAAGAATGATCACGATAAAAGTATATTTATAAATATGCTCAAACAAGCTGATGTATTAGCTGAGAATTTTCGCCCAGGTACAATGGAAAAACTGGGGTTTTCATGGGAAACGCTTCAAGAAATCAACCCGCGCCTCATATATGCTTCATCGTCAGGTTTCGGACATACCGGTCCGCTAAAAGATGCTCCTGCCTACGATACCATCATTCAGGCAATGAGCGGGATAATGATGGAAACAGGATATCCTGATGCTCCGCCAGTGCGCGTTGGTACATCTCTTGCGGATCTATGCGGCGGTGTCTATTTATTCAGCGGAATAGTGAGTGCACTTTATGGCCGCGAAAAGAGCCAGAGAGGGGCGCATGTCGATATAGCGATGTTTGATGCCACGCTGAGTTTTCTGGAGCATGGTCTGATGGCATATATCGCAACTGGGAAGTCACCACAACGTCTGGGAAATCGCCATCCCTACATGGCACCTTTTGATGTTTTCAATACTCAGGATAAGCCGATTACGATTTGTTGTGGTAATGACAAGCTTTTTTCTGCGTTATGCCAGGCACTGGAGCTTACGGAACTGGTTAATGATCCCCGATTTAGCAGCAATATTTTACGCGTACAAAACCAGGCTATTCTTAAACAATATATTGAGCGGACGTTAAAAACGCAGGCAGCTGAAGTTTGGTTAGCCAGAATACATGAAGTTGGTGTACCCGTCGCGCCGTTATTAAGTGTGGCTGAGGCCATTAAATTGCCACAAACTCAGGCGAGAAATATGTTGATTGAAGCCGGGGGAATAATGATGCCGGGTAATCCGATAAAAATCAGCGGCTGCGCGGACCCGCATGTTATGCCGGGAGCGGCAACGCTCGACCAGCATGGGGAACAAATTCGCCAGGAGTTCTCATCATAA
SEQ ID NO:3(来自酿酒酵母S288C的甲酸脱氢酶；EC 1.2.1.2；UniProtAccession Q08911；Sc_FDH1)：
ATGTCGAAGGGAAAGGTTTTGCTGGTTCTTTATGAAGGTGGTAAGCATGCTGAAGAGCAGGAAAAGTTATTGGGGTGTATTGAAAATGAACTTGGTATCAGAAATTTCATTGAAGAACAGGGATACGAGTTGGTTACTACCATTGACAAGGACCCTGAGCCAACCTCAACGGTAGACAGGGAGTTGAAAGACGCTGAAATTGTCATTACTACGCCCTTTTTCCCCGCCTACATCTCGAGAAACAGGATTGCAGAAGCTCCTAACCTGAAGCTCTGTGTAACCGCTGGCGTCGGTTCAGACCATGTCGATTTAGAAGCTGCAAATGAACGGAAAATCACGGTCACCGAAGTTACTGGTTCTAACGTCGTTTCTGTCGCAGAGCACGTTATGGCCACAATTTTGGTTTTGATAAGAAACTATAATGGTGGTCATCAACAAGCAATTAATGGTGAGTGGGATATTGCCGGCGTGGCTAAAAATGAGTATGATCTGGAAGACAAAATAATTTCAACGGTAGGTGCCGGTAGAATTGGATATAGGGTTCTGGAAAGATTGGTCGCATTTAATCCGAAGAAGTTACTGTACTACGACTACCAGGAACTACCTGCGGAAGCAATCAATAGATTGAACGAGGCCAGCAAGCTTTTCAATGGCAGAGGTGATATTGTTCAGAGAGTAGAGAAATTGGAGGATATGGTTGCTCAGTCAGATGTTGTTACCATCAACTGTCCATTGCACAAGGACTCAAGGGGTTTATTCAATAAAAAGCTTATTTCCCACATGAAAGATGGTGCATACTTGGTGAATACCGCTAGAGGTGCTATTTGTGTCGCAGAAGATGTTGCCGAGGCAGTCAAGTCTGGTAAATTGGCTGGCTATGGTGGTGATGTCTGGGATAAGCAACCAGCACCAAAAGACCATCCCTGGAGGACTATGGACAATAAGGACCACGTGGGAAACGCAATGACTGTTCATATCAGTGGCACATCTCTGGATGCTCAAAAGAGGTACGCTCAGGGAGTAAAGAACATCCTAAATAGTTACTTTTCCAAAAAGTTTGATTACCGTCCACAGGATATTATTGTGCAGAATGGTTCTTATGCCACCAGAGCTTATGGACAGAAGAAATAA
SEQ ID NO:4(来自动物双歧杆菌乳酸亚种DSM 10140的甲酰辅酶A:草酸辅酶A转移酶(FCOCT)；EC 2.8.3.16；UniProt Accession B9X295；Ba_frc)：
ATGGCTGACAAAAGCACTGCCCCACTGGCAGGCATCAAGGTAATCGACTGGACGCAGGTTCAGTCTGGCCCTTCGTGCACCCAGATTCTCGCATGGCTGGGCGCCGAGGTCATCAAGCTCGAGAAGGTGCATGGCGGCGACCCGACCCGTAACGAGATGAACGACGTCGACGGCTCGTACTCGCTGTACTTCCTGCAGCTCAACGCCAACAAGAAGTCGATCACGCTCGACATGAAGGACCCGGAAGGCAAGAAGATCCTCACCGACCTGCTCAAGGACGCAGACGTGTTCGTAGAGAACATCGGCCCAGGCGACGTGGAAAAGCTCGGCTTCGGTTGGGACGAGGTCCACAAGATCAACCCGAAGCTCATCATGGCCTCGCTCAAGGGATTCAACCAGGGCAGCCGCTTCGAACATGTCAAGGCCTTCGAGCCTGTCGCCCAGTGCGCAGGCGGCGCTGCTTCGACCACCGGCTGGTGGGAAGGCGACAAGAACATCCCGACGCAGTCCGGCGCTGCCCTTGGTGATTCGAACACCGGTATGCACCTGACGATTGCAATCCTCACCGCACTGCTGCAGCGCGAGCGCACCGGCGAAGGCGTGTTCGTGTACCAGTCCATGCAGAATGCAGTGCTCAACCTATGCCGCATCAAGCTGCGTGATCAGCTCATTCTCGATCACCTACACCAGCTTTCCTACTACGACTGCTACCCGGGCTACAAGTTCGGCAAAGCGATTCCGCGTGCGGCCAATGCCGAGGGCGGACTCGTGCTCGGCTGGTGCTATCGCGCCAAGGGCTGGGAAACCGATCCGAACGCCTATGTGTACATTGTGATTCAGCAGTCGCAGAAGGGATTCGAGAACTTCTGCAACGCCATGGGCTTCCAGGATTGGCTCACCGATCCGAAGTTCAGCACGCCGAACGCTCGCGATGAGCACAAGCAGGAAGTGTACAAGCGCGTCGAGGAATACACGATGCAGTACGACAAGTACACGCTCACCAAGGAACTCGGCGCCAAGGGCGTTCCGGTGGGCCCGGTGCTCGATTGGAACGAACTCGAAAACGATCCGGATCTCAACGAGGATGGCACGCTGATCACGATCGATCAGGGCGACGCCCGCGGCAAGTTCAAGACGATCGGCCTGCCGTTCACGATGAGCAACTATGCGCCGGATTACCAGCGCGCGCCGAAGCTCGGCGAGAACAACGAGGAGATTCTCAAGTCCCTCGGCTACACCGACGAGCAGATCGCCGATCTGGCGACCAAGGGCGTCATCGGCTCGAACGATGGCGTCAAGGCAGACCTCACCGCCGCTCCGGCACAGGCCTGA
SEQ ID NO:5(来自草酸盐贪铜菌ATCC 11883的甲酰辅酶A:草酸辅酶A转移酶(FCOCT)；EC 2.8.3.16；Co_frc1)：
ATGCCTGCACAACACACCCATGCCACGCCCGCCGGCCCGCTCGACGGCATCCGCGTGCTCGACCTGACCGCGGTCGTGCTCGGCCCGCTGGCCACGCAGGTGCTGGCCGATTTTGGCGCCGACGTGATCAAGATCGAGGGGCCGGAAGGCGACCTGATGCGCGCCAACGGCGTGTCGCAGCACGCCGGCATGAGCTCGATCTACCTGGCGCTGAACCGCAACAAGCGCTCGGTCGTGCTCGACCTGAAAATCCCCGAGGGCGCCGAAGCGCTGCGCGCGCTGATCGCCGGCGCCGACGTGCTGGTGCACAACATGCGCGTGGCGGCGATCGAGCGGCTTGGTTTTGGCTACGAGGCCGTCGCACGCATCAACCCGCGCATCGTCTACTGCGTCGCCACCGGCTTCGGCCAGGACGGCCCGCACCGCGACAAGCCCGCCTTCGACGACATCATCCAGGCCGGCTGCGGACTGGTGGCGCTGGGCGCCGGCAACGGCGAGCGGCCGGAATACGTGCCCAGCCTGATCGCCGACAAGACCACCGGGCTGGCGCTGGCCAATGCCGTGCTGGCCGCGCTGCTGCACCGCGAACGCCATGGCGTGGGCCAGTCGGTCGAAGTGCCGATGCTGGAAACCATGGCCGCCTTCGTGATGACCGAGCACCTGGGCGGGCTGACCTTCGAGCCCGCCCCCGCCGGCGCGGGCTATGCGCGGCTACTGCAGGGCGGCCGCCGCCCGGCGCCGACGCGCGACGGCTGGATCTGCGCGCTGCCCTATACCGAGCGCCACTGGCATGCCTTCTTCCGCGCGGTGGGTCGCGATGACCTTGCCGACCGTTACGAAGTCGGCGACCGCGCACAGCGCAATGCCAATATCCGCGCGCTGTACGGCCATCTTGCCGAACTGACCCCGGAGCGCAGCACCGCAGAATGGATGGCGCTGTTCGAAGCGCTCGACATCCCCGCCACGCCGATCTACGACCTCGATGCGCTGGTCGACCATCCGCACCTGCGCGCGGTGGGACTGTTCCAGGCCACGCAGCACCCCACCGAAGGCCCGCTGCGCGAAGTGCGCCCGGCCGCGCGCTTCTCGGCCACGCCGCTGTCGTTGCGCCGGCATGCGCCGGCACTGGGCGAACACACCGCCGAAGTCTTGCAGGAAGCCGGCATCGCCCTGCCCGCCGCGCATGCCGGCACACCCGCCGGCGCTCAGGAATAA
SEQ ID NO:6(来自草酸盐贪铜菌ATCC 11883的甲酰辅酶A:草酸辅酶A转移酶(FCOCT)；EC 2.8.3.16；Co_frc2)：
ATGAACCTCCCGCTCAACGGCATCAAGATCATCGACTTCACGCACGTCCAGGCCGGCCCCGCGTGCACGCAGCTGCTGGCATGGTTCGGCGCAGACGTCATCAAGGTGGAGCGCCCCGGTTCCGGCGACGTGACGCGCACGCAGCTGCGCGACATCCCGGATGTCGACGCGTTGTACTTCACCATGCTCAACTCCAACAAGCGCAGCCTGACGCTGGACACCAAGAAGCCCGAAGGCAAGAAGATCCTGGAGCAGCTGATCCGCGAGTCGGACGTGCTGGTCGAGAACTTCGGCCCGGGTGCGCTGGACCGCATGGGCTTCTCGTGGGAACGCATCAACGAGCTGAACCCGAAGATGATCGTGGCCTCGGTCAAGGGCTTCAGCGACGGCCATCACTATGAAGACCTGAAGGTCTATGAAAACGTGGCGCAGTGCGCGGGCGGTGCGGCGTCGACCACGGGCTTCTGGGATGGTCCTCCCACCGTTTCCGCAGCGGCGCTCGGCGATTCGAACACCGGCATGCACCTGGCCATCGGCATCCTGACCGCGCTGATCGGCCGCGACAAGACCGGCAAGGGCCAGAAGGTGGCCGTGTCGATGCAGGACGCCGTGCTCAACCTGTGCCGCGTCAAGCTGCGCGACCAGCAGCGCCTGGACCGCCTGGGCTTCCTGGAAGAGTATCCGCAGTACCCGCATGGCAGCTTCAGCGACGTGGTGCCGCGCGGCGGCAACGCTGGCGGCGGCGGCCAGCCGGGCTGGGTGCTGAAGTGCAAGGGCTGGGAAACCGACCCCAACGCCTATATCTACTTCACCATCCAGGGCCATGCCTGGGAGCCGATCTGCAAGGCGCTCGGCAAGCCGGAATGGATCGACGATCCCAACTACGCCACCGCCAAGGCACGCCAGCCGCATATCTTCGATATCTTCAACACCATCGAGGAATGGCTCGCCGACAAGACCAAGTACGAGGCGGTGGACATCCTGCGCAAGCATGACATCCCGTGCTCGCCGGTGCTGTCGATGAAGGAGATCGCGGCCGATCCGTCGCTGCGCGCCAGCGGCAGCATCACCGAGGTGCCACACAAGGAGCGCGGCACGTACCTGACGGTGGGCAGCCCGATCAAGTTCTCCGACCTCAAGCCGGAAATCACGGGTTCGCCGCTGCTGGGCGAGCATAGCGAAGAAGTGCTGGCCGGCCTGGGGTACGGCACGGACGACATCAAGCGCCTGCGCGAGTCCCAGGTGATCTGA
SEQ ID NO:7(来自大肠杆菌MG1655的甲酰辅酶A:草酸辅酶A转移酶(FCOCT)；EC2.8.3.16；UniProt Accession P69902；Ec_frc)：
ATGTCAACTCCACTTCAAGGAATTAAAGTTCTCGATTTCACCGGTGTGCAATCTGGCCCATCTTGTACTCAAATGCTGGCCTGGTTTGGCGCTGACGTTATTAAAATTGAACGTCCCGGCGTTGGTGACGTAACGCGTCACCAGCTGCGAGATATTCCTGATATCGATGCGCTTTACTTCACCATGCTTAACAGTAACAAACGTTCTATTGAGTTAAATACCAAAACAGCGGAAGGCAAAGAGGTAATGGAAAAGCTGATCCGCGAAGCTGATATCTTAGTCGAGAACTTTCATCCAGGGGCCATTGATCACATGGGCTTCACCTGGGAGCATATTCAAGAAATCAATCCACGTCTGATTTTTGGTTCGATCAAAGGGTTTGATGAGTGTTCGCCTTATGTGAATGTAAAAGCCTATGAAAACGTTGCTCAGGCAGCGGGTGGCGCGGCATCCACTACGGGTTTTTGGGATGGTCCGCCGCTGGTAAGCGCTGCAGCGTTGGGTGACAGCAACACCGGAATGCATTTGCTGATCGGTTTACTTGCTGCTTTGCTGCATCGCGAAAAAACGGGGCGTGGGCAACGAGTCACCATGTCAATGCAGGATGCCGTATTGAACCTTTGCCGCGTGAAATTACGTGACCAGCAGCGTCTAGATAAATTGGGTTATCTGGAAGAATACCCGCAGTATCCGAATGGTACATTTGGTGATGCAGTTCCCCGCGGTGGTAATGCAGGTGGTGGCGGTCAGCCTGGCTGGATCCTGAAATGTAAAGGCTGGGAAACCGATCCTAACGCCTATATTTATTTCACTATTCAGGAGCAAAACTGGGAAAACACCTGTAAAGCCATCGGCAAACCAGAATGGATTACCGATCCGGCATACAGTACAGCCCATGCACGACAGCCACATATTTTCGATATTTTTGCTGAAATCGAAAAATACACTGTCACTATTGATAAACATGAAGCGGTGGCCTATTTGACTCAGTTTGATATTCCTTGTGCACCGGTTTTAAGTATGAAAGAAATTTCACTTGATCCCTCTTTGCGCCAAAGTGGCAGTGTTGTTGAAGTGGAACAACCGTTGCGTGGAAAATATCTGACCGTTGGTTGTCCAATGAAATTCTCTGCCTTTACGCCGGATATTAAAGCTGCGCCGCTATTAGGTGAACATACCGCTGCTGTATTGCAGGAGCTGGGTTATAGCGACGATGAAATTGCTGCAATGAAGCAAAACCACGCCATCTGA
SEQ ID NO:8(来自产甲酸草酸杆菌DSM 4420的甲酰辅酶A:草酸辅酶A转移酶(FCOCT)；EC 2.8.3.16；UniProt Accession O06644；Of_frc)：
ATGACTAAACCATTAGATGGAATTAATGTGCTTGACTTTACCCACGTCCAGGCAGGTCCTGCCTGTACACAGATGATGGGTTTCTTGGGCGCAAACGTCATCAAGATTGAAAGACGTGGTTCCGGAGATATGACTCGTGGATGGCTGCAGGACAAACCAAATGTTGATTCCCTGTATTTCACGATGTTCAACTGTAACAAACGTTCGATTGAACTGGACATGAAAACCCCGGAAGGCAAAGAGCTTCTGGAACAGATGATCAAGAAAGCCGACGTCATGGTCGAAAACTTCGGACCAGGCGCACTGGACCGTATGGGCTTTACTTGGGAATACATTCAGGAACTGAATCCACGCGTCATTCTGGCTTCCGTTAAAGGCTATGCAGAAGGCCACGCCAACGAACACCTGAAAGTTTATGAAAACGTTGCACAGTGTTCCGGCGGTGCTGCAGCTACCACCGGTTTCTGGGATGGTCCTCCAACCGTTTCCGGCGCTGCTCTGGGTGACTCCAACTCCGGTATGCACCTGATGATCGGTATTCTGGCCGCTCTGGAAATGCGTCACAAAACCGGCCGTGGTCAGAAAGTTGCCGTCGCTATGCAGGACGCTGTTCTGAATCTGGTTCGTATCAAACTGCGTGACCAGCAACGTCTGGAAAGAACCGGCATTCTGGCTGAATACCCACAGGCTCAGCCTAACTTTGCCTTCGACAGAGATGGTAACCCACTGTCCTTCGACAACATCACTTCCGTTCCACGTGGTGGTAACGCAGGTGGCGGCGGCCAGCCAGGCTGGATGCTGAAATGTAAAGGTTGGGAAACCGATGCGGACTCCTACGTTTACTTCACCATCGCTGCAAACATGTGGCCACAGATCTGCGACATGATCGACAAGCCAGAATGGAAAGACGACCCAGCCTACAACACATTCGAAGGTCGTGTTGACAAGCTGATGGACATCTTCTCCTTCATCGAAACCAAGTTCGCTGACAAGGACAAATTCGAAGTTACCGAATGGGCTGCCCAGTACGGCATTCCTTGCGGTCCGGTCATGTCCATGAAAGAACTGGCTCACGATCCTTCCCTGCAGAAAGTTGGTACCGTCGTTGAAGTTGTCGACGAAATTCGTGGTAACCACCTGACCGTTGGCGCACCGTTCAAATTCTCCGGATTCCAGCCGGAAATTACCCGTGCTCCGCTGTTGGGCGAACATACCGACGAAGTTCTGAAAGAACTGGGTCTTGACGATGCCAAGATCAAGGAACTGCATGCAAAACAGGTAGTTTAA
SEQ ID NO:9(来自Bacteroides subtilis 168的草酸脱羧酶(OXDC)；EC4.1.1.2；UniProt Accession O34714；Bs_oxdC)：
ATGAAAAAACAAAATGACATTCCGCAGCCAATTAGAGGAGACAAAGGAGCAACGGTAAAAATCCCGCGCAATATTGAAAGAGATCGGCAAAACCCTGATATGCTCGTTCCGCCTGAAACCGATCATGGCACCGTCAGCAATATGAAGTTTTCATTCTCTGATACTCATAACCGATTAGAAAAAGGCGGATATGCCCGGGAAGTGACAGTACGTGAATTGCCGATTTCAGAAAACCTTGCATCCGTAAATATGCGGCTGAAGCCAGGCGCGATTCGCGAGCTTCACTGGCATAAAGAAGCTGAATGGGCTTATATGATTTACGGAAGTGCAAGAGTCACAATTGTAGATGAAAAAGGGCGCAGCTTTATTGACGATGTAGGTGAAGGAGATCTTTGGTACTTCCCGTCAGGCCTGCCGCACTCCATCCAAGCGCTGGAGGAGGGAGCTGAGTTCCTGCTCGTGTTTGACGATGGATCATTCTCTGAAAACAGCACGTTCCAGCTGACAGATTGGCTGGCCCACACTCCAAAAGAAGTCATTGCTGCGAACTTCGGCGTGACAAAAGAAGAGATTTCCAATTTGCCTGGCAAAGAAAAATATATATTTGAAAACCAACTTCCTGGCAGTTTAAAAGATGATATTGTGGAAGGGCCGAATGGCGAAGTGCCTTATCCATTTACTTACCGCCTTCTTGAACAAGAGCCGATCGAATCTGAGGGAGGAAAAGTATACATTGCAGATTCGACAAACTTCAAAGTGTCTAAAACCATCGCATCAGCGCTCGTAACAGTAGAACCCGGCGCCATGAGAGAACTGCACTGGCACCCGAATACCCACGAATGGCAATACTACATCTCCGGTAAAGCTAGAATGACCGTTTTTGCATCTGACGGCCATGCCAGAACGTTTAATTACCAAGCCGGTGATGTCGGATATGTACCATTTGCAATGGGTCATTACGTTGAAAACATCGGGGATGAACCGCTTGTCTTTTTAGAAATCTTCAAAGACGACCATTATGCTGATGTATCTTTAAACCAATGGCTTGCCATGCTTCCTGAAACATTTGTTCAAGCGCACCTTGACTTGGGCAAAGACTTTACTGATGTGCTTTCAAAAGAAAAGCACCCAGTAGTGAAAAAGAAATGCAGTAAATAA
SEQ ID NO:10(来自大麦(Hordeum vulgare)的草酸氧化酶(OXO)；EC 1.2.3.4；UniProt Accession P45850；Hv_OxOx)：
ATGTCCGACCCAGACCCACTCCAGGACTTCTGCGTCGCGGACCTCGATGGCAAGGCGGTTTCGGTGAACGGGCATACGTGTAAGCCCATGTCGGAGGCCGGCGACGACTTCCTCTTCTCGTCCAAGCTGACCAAGGCCGGCAACACGTCCACCCCGAACGGCTCGGCCGTGACGGAGCTCGACGTGGCCGAGTGGCCCGGTACGAACACGCTGGGCGTGTCCATGAACCGTGTGGACTTCGCGCCGGGGGGCACCAACCCGCCGCACATCCACCCGCGTGCAACCGAGATCGGCATGGTGATGAAAGGTGAGCTCCTCGTTGGAATCCTCGGCAGCCTTGACTCCGGAAACAAGCTCTACTCCAGGGTGGTGCGTGCCGGAGAGACTTTCGTCATCCCGCGCGGCCTCATGCACTTCCAGTTCAACGTTGGTAAGACGGAAGCCTACATGGTTGTGTCCTTCAACAGCCAGAACCCTGGCATCGTCTTCGTGCCGCTCACACTCTTCGGCTCCGACCCTCCCATCCCCACGCCCGTGCTCACCAAGGCTCTCCGGGTGGAGGCCGGAGTCGTGGAACTTCTCAAGTCCAAGTTCGCCGGTGGGTCTTAA
SEQ ID NO:11(来自热醋穆尔氏菌(Moorella thermoacetica)ATCC 39073的草酸氧化还原酶(OOR)α亚基；EC 1.2.7.10；UniProt Accession Q2RI41；Mt_OORA！)：
ATGGGTAAAGTAAGGAATATCTCTGGATGTGTGGCGGTAGCACATGGTGTGCGGTTAGCCGATGTCGACGTTATCTGTTCCTATCCCATCCGGCCTTATACCGGTATCATGTCCGAGCTGGCACGGATGGTTGCCGACGGCGAGTTGGATGCCGAATTTGTCCATGGCGAAGGTGAACACGCCCAGTTGAGTGTCGTTTACGGCGCTTCGGCTGCCGGAGCACGGGTATTCACCGGTAGTTCCGGTGTCGGCGTAACCTATGCCATGGAAGTCTATTCACCTATCTCTGGTGAACGTTTACCGGTACAGATGGCCATTGCCGACCGGACCCTGGACCCGCCCGGTGACTTTGGTGAGGAACATACCGATGCCGAGTGTTGCCGGGACCAGGGCTGGATCCAGGGTTGGGCCTCCACTCCCCAGGAAGCCCTGGATAACACCTTGATTTACTACCGGGTAGGTGAAGACCAGCGGGTCTTGTTGCCCCAGTATGCCTGCCTGGACGGGTACTTCGTCAGCCATATCCTAGGCCCGGTGGATATTCCCGACGAGGCCCAGGTGAAAGAGTTCCTGCCGCCTTACAAGAATCACCATGTCCTCGATCCCAGGAAGCCCCAGATTATCGGCCCTCAAATTGAGCCGGCCATGGGACCTCCCCTCCAATACCAGCGTTACCAGGCCGTTAAAGGCGTGCACAAGGTTCTAGAAGAGGCCTGTGACGAATTCGCCCGGATTTTCGGCCGCAAGTACGATCCCTACCTTGATGAATACCTGACTGACGATGCCGAGGTTATAATCTTCGGCCAGGGCGCCCACATGGAGACTGCTAAAGCGGTAGCCCGGCGCCTGCGCAACCTTGGTGAAAAGGTAGGAGTAGCGCGGCTACGTACCTTCCGGCCCTTCCCGACGGAACAAATTAAAGAGCGACTTTCTAAATTCAAGGCTATTGGTGTCCTCGATGTTTCGGCCAACTTCGGCATTTCCTGCAGCGGCGGCGTCCTCCTGTCCGAACTGCGGGCCGCCCTCTATGACTACGGCGACAAAGTAAAGACCGTAGGCTTTGTAGCCGGCCTAGGCGGCGAAGTCGTCACCCACGATGAGTTCTACCGCATGTTCCAGAAACTAAAGGAGATCGCCAAGACAGGTAAAGTCGAGCAGACCTCTTATTGGATCCCCTTTGAATTATAA
SEQ ID NO:12(来自热醋穆尔氏菌ATCC 39073的草酸氧化还原酶(OOR)β亚基；EC1.2.7.10；UniProt Accession Q2RI42；Mt_OORB)：
ATGCTGGATCGGATTGCATCTATTAAAAAGGCACCTGATGAGGAATACTATGTTCCCGGGCATCGTACCTGTGCCGGCTGCGGCCCGGCCCTGACCTATCGCCTGGTGGCCAAAGCCGCCGGTCCCAACACCATCTTCATTGGTCCCACCGGCTGCATGTACGTGGCCAATACCAGCTACGGCTGCGGTCCCTGGCGGGTACCCTGGATCCATGCCCAGATCACCAATGGCGGCGCCGTGGCCTCCGGTATTGAGGCCGCCTATAAGGCTATGATCCGCAAGAAGAAGACCGACGCCGAGTTCCCGAACATTATCGTTATGGCCGGTGATGGCGGCGCCGTCGATATCGGCCTCCAGGCACTGTCGGCCATGCTCTATCGCGGTCATGACGTCCTCTTTATCTGCTACGATAACGAGTCCTATGCCAATACCGGTATTCAGACCTCCCCGACGACGCCCTACGGGGCTAATACGACTTTTACCCCGCCTGGTGAGGTGGTACCGGAGGGCAAGAAGCTCTTCCCGAAGGATAACCCCAAGGTTATCGCCCACGGTCACCCGGAACTGAAGTATGTCGCCACGGCCTCCATCGGCTGGCCGGTAGACCTGATGAACAAGGTCCGTAAGGGCTTAAACCAGGAAGGTCCGGCCTATATCCACATCCACGCTCCTTGCCCCAAGGGCTGGCAGTTCCCGGCCGACAAGACCATTGAAATGGCCAAATTGGCCGTCCAGACGGGCATGTTCCAGCTCTACGAATATGAGAATGGTGAGTATAAACTGTCGGTTAAAGTCGATAAACGGAAACCGGTCAGCGAGTACATGAAACTCCAGAAGCGGTTTGCTCACCTGAAGCCCGAGCATATCGCCAAGATGCAGGCCTTTGTCGACGCCCGCTGCGCTGAAGTAGGTATCACCGTGCCGGTAGTCGCTTCCAACGCTTAA
SEQ ID NO:13(来自热醋穆尔氏菌ATCC 39073的草酸氧化还原酶(OOR)δ亚基；EC1.2.7.10；UniProt Accession Q2RI40；Mt_OORD)：
ATGTCCACCAAAGATCTCTTTGCTGAACCCAATCTCAAGCAGATTACTGTGTGGGCCCGGGGCGTTGTCATGAACAAGGACGCCCGTGATATCGTGGTGGCCCTGACAGAAGCGGCTGCCAAAGAGGGTAAATATGTTCAGGCCTGGGAGAACTATGTTGACCTCCCCGACCGGATCTACGTCCCGGTAAGGGCCTATGCCCGCATCAGCAGCGATCCCATTGAAAGCAAATACATTTACGAGAATGAGACTCCCGACATTGTTGTTTTAGTTGAAGAATCCCTGATCAAAGGAGTGCCCATACTGAAGGGCATCCGTCCGGGTAGTACCCTGGTTGTAAATACCAAACGTTCCATAGATACTATCCTTGAGTTCCTGGGTGATACCGGCAATCTGGCCCAAATCGTAACGGTGGATGCCAACAGTATGGCCGAGGCTGTCATGACTTTGTCTGGCGCTGAAGGCGCTACTGACGCTACGGGTATTGGCGCCGGCATAGCGGCTCCCATTGCGGGTGCTGTTGTCAAGGCTACAGGTATAGTTGATGTTGAAAACCTCGCCGCTGTGGTCAAGAATCCCGCCGCCATGCGCCGGGGCTATGCGGAAGCCCAGGTGCGCCAGCTGCCGCCCCATGAAGCAGTTGAGGAAGCGGCCGTATCAGCGACTGAATTGTTGAGGCAAATGCCCTTTGCCGGGACGGTTCCCTCACCGGTAACGGAGAATGAGGGCATGGTAACCGGCAACTGGCGCATCCAAAGGCCCATTATCGACCGGGAAGCCTGTACCGAATGTTATACCTGCTGGATCTACTGCCCCGATTCCTGCATCACCAGGACCGAAGAAGGACCGGTATTTAATATGAAGTACTGCAAGGGCTGCGGCCTCTGTACGGCGGTCTGCCCCAGCGGCGCCCTGACTAATGTACCGGAACTTGACTTCAAAGATTAA
SEQ ID NO:14(来自拟南芥col-1的草酸盐--辅酶A连接酶/草酰辅酶A合成酶(OXS)；EC 6.2.1.8；UniProt Accession Q9SMT7；At_AAE3)：
ATGGATAGCGATACTCTCTCAGGATTATTGGAAAACGTCGCCAAAAAATTCCCCGATCGCCGAGCTCTCTCCGTTTCTGGAAAATTCAATCTCACTCACGCGCGTCTTCACGATCTAATCGAACGCGCCGCTTCACGCCTTGTCTCCGACGCTGGAATCAAACCCGGCGATGTCGTTGCTCTCACCTTCCCTAACACCGTCGAGTTTGTTATAATGTTTTTAGCGGTGATAAGAGCTAGAGCCACGGCGGCGCCGTTGAACGCAGCGTACACGGCGGAGGAATTTGAGTTTTACCTCTCCGATTCAGATTCAAAGCTATTGTTAACCTCTAAAGAAGGAAACGCACCGGCTCAAGAAGCAGCTTCAAAGCTGAAAATCTCTCACGTCACCGCTACGCTGCTTGACGCTGGCTCGGACCTTGTACTATCCGTTGCGGATTCAGATTCCGTCGTTGACTCAGCGACGGAACTCGTTAATCACCCGGACGACGGTGCTCTCTTCCTCCACACTTCTGGCACTACGAGCCGTCCAAAGGGTGTACCGCTTACGCAGCTCAATCTAGCTTCATCCGTCAAGAACATTAAAGCTGTGTACAAGCTTACTGAGTCTGATTCTACGGTGATTGTTCTCCCTCTGTTCCATGTTCATGGATTGTTAGCTGGGTTGCTTAGCTCGCTTGGAGCTGGTGCTGCTGTAACTCTTCCAGCTGCTGGTAGATTCTCAGCAACAACATTTTGGCCAGATATGAAGAAGTATAACGCTACATGGTATACTGCTGTGCCGACCATTCATCAGATCATATTGGACCGCCACGCGAGCCACCCTGAAACGGAATATCCTAAACTCCGGTTCATCAGGAGTTGCAGTGCTTCTTTGGCTCCGGTGATATTGTCCAGGCTTGAGGAAGCGTTTGGAGCACCGGTGCTCGAGGCCTATGCAATGACAGAGGCAACACATTTGATGAGCTCAAACCCCTTACCAGAGGAAGGTCCACACAAGCCTGGGTCTGTTGGGAAACCGGTAGGTCAAGAAATGGCGATCCTTAATGAGAAAGGCGAGATCCAAGAGCCAAATAACAAAGGAGAGGTTTGTATAAGAGGTCCAAATGTGACCAAGGGTTACAAGAATAACCCAGAAGCCAACAAGGCAGGTTTCGAGTTTGGGTGGTTCCACACTGGTGATATCGGTTACTTTGATACCGATGGGTATTTGCATCTGGTGGGTCGGATCAAAGAGCTTATTAACCGTGGAGGTGAGAAGATATCTCCAATTGAAGTGGATGCAGTACTCTTAACGCATCCTGACGTTTCTCAGGGTGTTGCATTCGGTGTTCCTGATGAGAAATATGGGGAAGAGATTAACTGTGCGGTGATTCCAAGAGAAGGAACTACTGTAACCGAAGAGGACATTAAAGCGTTTTGTAAGAAGAATTTGGCAGCTTTCAAGGTGCCAAAGAGAGTGTTCATCACTGATAACCTCCCCAAAACTGCCTCTGGTAAGATTCAGCGCCGTATCGTCGCACAACATTTCCTTGAGAAGCCCTGA
SEQ ID NO:15(来自扭脱甲基杆菌AM1/DSM 1338的草酸盐--辅酶A连接酶/草酰辅酶A合成酶(OXS)；EC 6.2.1.8；UniProt Accession C5APP0；Me_oxs)：
ATGACTATGTTATTACCTGAACCATCAGCTTTGTTACCAACAGATTCAAAGGAAGCCTCAGTCCCAGCAACAACATTGCATGAGTTGATACAAGCTGGTGCAGACGCTGCCCCTGCCTTATCTTCTCCAGGAGGAGTACCATTAACCTTTCAAGCATTAAGAGCCTTAACCGAGAGAACCGTCGCCGACTTGAACGCAAGGGGTATTGGTAGAGGTGATAGAGTTGCCATTGTTTTGCCAAATGGACCAGAAATGGCTGCAGCTTTCATTGCAGTAGCAGCCGGTACAACTTCAGCACCTTTAAATCCTTCTTACAAGGCGGACGAATTTGAGTTCTATATGTCAGATTTAGGTGCAAAATTATTATTAGTCGCAGAGGGTTCTGAGACTCCTGCCGTTGCCGTGGCTGAGAAATTGGGTGTTTCAGTAGCTAGATTAAGACCAACTCCAGATGAAGGAGCAGGTTCTTTTACTTTACATTTTGCCTCAGAGTCTACTGGTCCAACCGAAAAGTCAGGTCCTGCAGGTTCAGATGATATAGCTTTGGTATTGCACACATCAGGTACCACATCTAGGCCTAAGATTGTCCCATTGACTCAAGCAAATGTATGCGCATCTGCTAGAAACATTAGGACTGCATTAGCCTTTGGCCCGGAAGATAGAGGATTAAACATAATGCCTTTGTTCCATATACACGGATTAATCGCTGGAATTTTAGCCCCATTATCTGTCGGTGGTCAAGTCTCTTGCACTCCAGGTTTCAACGCATTGAAATTCTTTGGATGGATGGATGAAGTCAATCCAACCTGGTACACCGGAGTACCAACAATGCATCAAGCCATTTTAGGAAGGGCAGCAAGAAATAAAGAAATTATCGCCAGGAACCCTTTAAGATTTATCAGATCTTCTTCATCATCTTTGCCACCACAAGTCATGAAAGAGTTGGAGGAAACCTTCGGTGCTCCTGTTATTGAAGCATACGGAATGACCGAAGCTGCACATCAAATGGCCTCAAACCCATTACCTCCTAAGCCTCACTATGCTGGTTCTGTAGGTTTGGCTGCAGGTCCTGAGATTGCTGTTGTCGACTTAGACGGTGAACCTTTACCAGCTGGAGAGACAGGTGAAATAGTCATAAGAGGTGACAACGTTATGAAGGGTTACGAAAACAACGAGAAGGCAAATGCAGAAGCATTCACCAAGCAGGGTTGGTTCAGAACTGGTGACCAAGGTGTCTTGTCTCCAGAGGGATACTTATCTATCACTGGAAGGTTAAAGGAGATAATCAATAGGGGTGGTGAGAAAATCTCACCAAGAGAAGTAGATGAAATTTTGATGGACCATCCTGCAGTATCACAATGCGTTACATTTGCGGTTCCTCATGATAAATTGGGTGAAGATGTTGCTGCTGCCATCGTCTTAAGGGAGGGTGTTGAAGCCGTTGAAAAGGACATTCGTAGCTTCGCTTCAGAAAGATTAGCAGCATTTAAAGTACCTGCCAAAATCTTAATTTTGGATGAAATCCCAAAAGGTGCCACAGGTAAGTTGCAAAGAATAGGTTTGGCACAGAAATTGGGATTGGTTTAA
SEQ ID NO:16(来自酿酒酵母S288C的草酸盐--辅酶A连接酶/草酰辅酶A合成酶(OXS)；EC 6.2.1.8；UniProt Accession P38137；Sc_YBR222C)：
ATGACAAGTGCCGCTACTGTTACTGCTTCGTTCAACGATACTTTTAGCGTATCCGATAATGTCGCCGTTATTGTTCCTGAAACGGACACTCAGGTGACCTACAGGGATCTATCCCACATGGTGGGTCACTTCCAGACCATGTTCACAAATCCTAATTCTCCATTGTACGGAGCTGTTTTCAGACAAGATACAGTGGCGATATCCATGCGTAATGGGCTGGAATTTATCGTCGCTTTCCTCGGTGCTACTATGGACGCTAAAATTGGCGCGCCCTTGAATCCCAATTATAAGGAAAAGGAGTTCAATTTTTATTTGAATGACCTGAAATCTAAGGCGATTTGCGTCCCAAAGGGTACCACAAAGTTACAGAGTTCTGAAATTCTAAAATCTGCCTCCACGTTTGGATGTTTTATCGTAGAGCTGGCCTTCGATGCGACCAGGTTTAGGGTAGAGTATGATATATACTCTCCAGAGGACAACTACAAAAGGGTTATTTACCGGTCTTTGAACAACGCCAAATTTGTCAACACAAATCCCGTTAAATTCCCTGGGTTTGCCCGTTCCAGTGACGTTGCCCTGATTTTGCATACCAGTGGTACCACCTCCACTCCAAAAACGGTGCCTTTGTTACATTTGAACATTGTGAGAAGCACGTTGAACATTGCTAACACTTACAAGCTAACGCCCTTGGACAGATCTTATGTCGTGATGCCTCTTTTCCACGTCCATGGGTTAATTGGTGTTTTACTTTCCACTTTTAGAACTCAGGGTTCTGTTGTGGTTCCCGATGGATTCCATCCAAAGTTATTCTGGGACCAATTTGTTAAGTACAACTGTAATTGGTTCAGTTGCGTTCCCACAATAAGCATGATTATGCTGAACATGCCCAAACCAAACCCTTTCCCACACATTAGATTCATCAGATCGTGTTCTTCTGCTTTGGCTCCAGCAACGTTCCATAAGCTGGAGAAGGAATTCAATGCACCTGTCTTGGAGGCCTATGCGATGACCGAAGCATCACATCAAATGACCTCAAACAATCTGCCTCCAGGAAAGAGAAAGCCTGGTACTGTGGGCCAGCCACAAGGAGTCACCGTCGTCATTCTAGATGACAATGACAATGTCTTGCCCCCGGGCAAAGTCGGCGAAGTTTCCATCAGAGGCGAAAACGTCACTTTGGGGTATGCTAATAATCCAAAAGCTAACAAGGAGAACTTCACCAAGAGAGAGAACTATTTCAGAACCGGTGACCAAGGTTATTTCGACCCTGAGGGGTTTTTGGTCCTTACAGGCAGAATCAAAGAGCTTATCAACAGGGGTGGTGAAAAGATTTCACCCATTGAGCTCGACGGCATTATGCTATCGCATCCAAAGATCGATGAAGCCGTTGCATTTGGTGTTCCCGACGATATGTACGGCCAAGTAGTTCAAGCCGCCATTGTTTTGAAGAAGGGAGAAAAAATGACCTACGAAGAACTGGTGAACTTCTTAAAGAAGCACCTAGCCTCTTTCAAAATTCCAACCAAGGTGTACTTTGTTGATAAGCTACCAAAAACCGCTACAGGTAAAATCCAGAGAAGAGTTATCGCAGAAACTTTTGCTAAGAGCAGCAGAAATAAGAGTAAGTTGTAA
SEQ ID NO:17(来自动物双歧杆菌乳酸亚种DSM 10140的草酸盐:甲酸盐反向转运蛋白(oxlT)；UniProt Accession B9X293；Ba_OxlT)：
ATGACAAAGATATTGATAGACGACATCATCCCCATCATCGTCATCATGGCGTTGGGCTATGTCTGTGGAAAACTCAGCTACTTCGACAACGATCAGCGTCAGGGCCTCAACAAGCTCGTGCTGAACATCGCCCTCCCTGCCGCACTGTTCATCTCGATCGTCAAGGCGACCCGTGAGATGCTCGCACAGGATGCGGTGCTCACAATTCTCGGCTTCATCGGCATCATCGTGATGTTCATGTTGAGCTACTACCTGTGCCGACTGATGTTCCACCATTCGATCCAGGAAGCGGCCGTATGCGCGTTGATCGCCGGCTCGCCGACCATCGGCTTCCTCGGCTTCGCCGTGCTCGACCCGATCTACGGCGACACCGTGAGCACGAACCTCGTCATCGCGATCATCTCGATCGTCGTCAACGCCGTCACGATTCCGATCGGCATGTATCTGATCAACCTCGGCCAGAGCAAGGATCGCGAGCGCCTGTCGAAGGCCGCCGTCACGAACTCGAAGGGCCAGGTCAGCATCGCCAATCCGAAGGACGATATCGCCGTCGATCCGAACAAGGATGCGAAGACCGACAAGACGGCAGAGGTGATGATCTCCAAGTCGTCGAACATGGGCAAGAAGAAGAACCAGAACCTCGAAGCGCTCATCAACGCACTCAAGCAGCCTGTGTGCTGGGCTCCGCTGCTCGCCATCGTGCTCGTGCTCATCGGCGTGCGCGTGCCGTCCGGTTTCGCTCCCACCTTCGACCTGATTGCCAAAGCGAACTCCGGTGTCGCCGTGCTGGCCGCCGGCCTCGCGCTCTCCACTGTGAAGTTCTCGCTGGGCTGGGAAACGATCTGGAACACGTTCTACCGCTTGATTCTCACCCCTGCGGCATTCCTCGGCGTCGGCCTGCTGCTGGGCATGGGCAGCAATGTGAACAAGCTCTCCATGCTCGTCATGGCTGTGGCCCTGCCGCCTGCATTCTCCGGCATCATCATCTCGAGCCGCTACAACATCTACGTCAAGGAAGGTGCGTCGACCACCGCGGTTTCCACGGTTGCGTTCGCCGTCACCTGCCTGCTGTGGATCTGGCTCGTGCCGCTGTGCTGCCACTGA
SEQ ID NO:18(来自草酸盐贪铜菌ATCC 11883的草酸盐:甲酸盐反向转运蛋白(oxlT)；Co_OxlT1)：
ATGAGTAACGCAACGGCAGGGGCCCTGAGCCCCAATCAGGGAGCTTCGGAATCCACGCGCTGGATCCAGCTGCTCGTCGGCGTGGTCTGCATGATCGCCACGGCCAACATCCAGTACGCCTGGACGCTGTTCGTGCCTGAGATCCAGGATACCTACGGCTGGTCACGCGCCAGCATCCAGATTGCCTTTACCGTGTTCGTGCTGGTGCAGACCTGGCTGGCGCCGATCGAGGGCTACTTCATCGACAAGTTCGGGCCGCGCATGATGGTGGCCTTCGGCGCGCTCTTTATCGGCACGGCGTGGGTCATCAACTCGCAGGCGACCACGCTGATGGGCTTCTACGTCGGCGCCGCCGTCGGCGGCCTGGGCGTGGGCTCCATCTACGCGACCTGCATCAACAACGCGCTGAAGTGGTTCCCGGACCGCCGCGGCCTCGCGGTCGGCCTGACCGCCGGCGGCTATGGCGCGGGCTCGGCGGCGACCATCCTGCCGATCGCGGCGATGATCGAGTCGCAGGGTTTCCAGCACACCTTCCTGTTCTTCGGCCTGCTGCAGGGGTCGCTGGCCTTTGTCGCGGCGTGGTTCCTGCGCTCGCCCAAGACCGGCGAGGTGCGGGGGTCGAAGAAGCTGGCCCAGGCCACGCGCGACTACACCCTCAAGGAAGCGCTGTGCACCAGGCTGTTCTGGCTGATGCTGGTGATGTTCGTGCTGGTGGTCACGGGCGGCATGATGGCCGTGGCGCAGCTCGGTGTCATCGCCAAGGACCTTGGCGTGAAGGAATTCCAGGTCGACCTGCACTTCTTCGTGATGGCTGCACTGCCGCTCGCCCTGATGCTGGACCGCATCATGAACGGCATCTCGCGTCCGCTGTTCGGCTGGATCTCGGACAACATCGGCCGTGAAAAGACCATGGTGATCGCCTTTACGCTGGAAGGGCTGGGCATCATCGCGCTGGGCTACTTCGGCAGCAACCCGTACGCCTTCCTGATCCTGTCCGGCGTGGTGTTCCTGGCCTGGGGCGAAGTCTACTCGCTGTTCTCGGCACTGGCGGGCGATGCCTTCGGCACCAAGCACATCGGCAAGATCTACGGCGTGCTGTACACCGCCAAGGGCATCGGCGCACTGTTCGTGCCGATCGGCAACCTGCTGATGGAAGCCACCGGCACGTGGTCGACGGTGCTCTACACGGTGGCGGTGATGGACCTGACCGCGGCGTTCCTTGCCATCATGGTGCTGCGGCCGGTGCTTGCCTCGCACGTTGCGACATCGAGGCAGCGGTTTGCGCAGGAATCTGCCGCGGCCGGCGCGCAAGTGGCGGCTTGA
SEQ ID NO:19(来自草酸盐贪铜菌ATCC 11883的草酸盐:甲酸盐反向转运蛋白(oxlT)；Co_OxlT2)：
ATGGAATTGCAGCAGAGGGAGTCCACGTCGCGCTTTGCGTCGCCGTGGGTGCAATTGGTCTTTGGCGTGATCTGCATGGCGATGATCGCCAACATGCAGTACGGCTGGACGCTGTTCGTCAACCCGATCGACGACAAATACCACTGGGGCCGCACCGCGATCCAGGTGGCATTCACCATCTTCGTCGTGACGGAAACCTGGCTGGTACCGATCGAGGGCTACCTCGTCGACAAGTACGGCCCGCGCCCGGTAGTGGTGGGCGGCGGGTTGCTGTGCGCCATCGCGTGGGCGCTGAACTCGGTGGCGTCCTCGCTGCCGATGCTGTACTTCGCGGCCGCAATCGGCGGCGTGGGTGCCGGCGCGGTCTACGGCACCTGCGTGGGCAATGCGCTCAAGTGGTTCCCCAACCGGCGCGGGCTGGCGGCGGGCATTACCGCAGCAGGCTTTGGCGCTGGTTCCGCGATGACCGTGGTGCCGATCGCCAACATGATCAAGACCTCGGGCTATGAAGCCACCTTCCTGTGGTTCGGGCTGGGGCAGGGCCTGATCGTCTTCATCCTCGGCCTGGCCCTGTATCCGCCGTCGGCCAGGATCCTGAGCGAGGTCAAGACCACGCTCAAGGCCGCCGCGACCTACAACGCCACGCCGCGCCAGGTGCTGAAGTCGCCGATCTTCTGGATCATGTACGCCATGTTCGTGATGATGGCCGCCGGCGGGCTGATGGCCACCGCGCAGTTGGGCCCGATCGCCAAGGACTTCGGCCTGCATGACTCGCCGGTGTCGATCCTCGGCCTGACGCTGCCCGCGCTGACCTTCGCGCTGACCATCGACCGCGTGCTCAACGGCCTGACCCGCCCGTTCTTCGGCTGGATCTCCGACCATATCGGCCGCGAGCGCACCATGTTCTTCGCCTTCGCGGTGGAGGCGGTCGGCATCCTGCTGCTGTCCAAGTACGGCCACAACCCGGTGGCGTTCGTGGTGCTGACCGGCATCGTGTTCTTTGCCTGGGGTGAGATCTACAGCCTGTTCCCGGCCACCTGCGGCGATACCTTCGGTCCCAAGTTCGCCGCCACCAACGCCGGCTTGCTGTACACCGCCAAGGGCACGGCTGCGTTGCTGGTGCCGTTCTCCAGCGTGATCACCGCCGCCACCGGCGACTGGCACGCGGTGTTCATGCTCGCCTCCGGCATGGCGGCGCTGTCGGCGGTGCTGGCGCTGTTCGTGCTCAAGCCGATGCGTGAAGCCCATGCCCGCAAGTATGTCCACGCCAATACCGCGGCGCCGATGGGCTATCGTGCCGTGCAGGAAGACCTGACCTGA
SEQ ID NO:20(来自大肠杆菌MG1655的草酸盐:甲酸盐反向转运蛋白(oxlT)；UniProt Accession P37662；Ec_YhjX)：
ATGACACCTTCAAATTATCAGCGTACCCGCTGGCTGACACTCATCGGTACTATCATTACCCAGTTTGCGCTGGGGTCGGTTTATACCTGGAGCCTGTTTAATGGCGCGCTTTCCGCCAAGCTGGATGCGCCGGTAAGCCAGGTCGCTTTCTCTTTCGGCTTGTTAAGTCTGGGGCTGGCAATTTCGTCTTCTGTTGCGGGCAAATTACAGGAACGTTTTGGCGTTAAACGCGTCACCATGGCTTCCGGCATTTTGCTGGGATTAGGCTTCTTCCTGACAGCGCATTCTGACAACCTGATGATGCTGTGGTTAAGCGCCGGTGTGCTGGTGGGACTGGCAGATGGCGCGGGTTATCTGCTGACGCTCTCTAACTGTGTGAAGTGGTTCCCGGAGCGTAAAGGTCTGATCTCCGCGTTCGCTATCGGTTCTTATGGTCTGGGTAGCCTGGGTTTCAAATTTATCGACACGCAGCTGCTGGAAACGGTCGGTCTGGAAAAAACCTTTGTGATTTGGGGAGCGATTGCGCTGTTGATGATTGTTTTCGGCGCAACGTTAATGAAAGACGCACCAAAACAGGAAGTGAAAACCAGCAATGGTGTGGTGGAGAAAGATTACACGCTGGCAGAGTCGATGCGTAAACCGCAGTACTGGATGTTAGCGGTAATGTTCCTGACCGCCTGCATGAGCGGCCTGTACGTGATTGGGGTAGCGAAAGATATCGCCCAAAGTCTGGCACACCTTGATGTGGTTTCCGCAGCCAATGCAGTCACTGTTATTTCCATCGCCAACCTTTCAGGTCGTCTGGTGCTGGGTATTCTGTCTGACAAAATCGCCCGTATCCGTGTTATTACCATTGGTCAGGTGATATCGCTGGTGGGTATGGCGGCCCTGCTGTTTGCACCATTGAATGCAGTGACGTTCTTTGCAGCGATTGCCTGCGTGGCATTTAACTTTGGCGGCACTATTACCGTCTTTCCGTCACTGGTCAGTGAGTTCTTTGGCCTCAATAACCTGGCGAAAAACTACGGTGTGATTTATCTCGGTTTCGGTATCGGTAGCATTTGTGGTTCGATTATCGCCTCACTGTTTGGCGGCTTCTATGTGACCTTCTACGTGATTTTCGCCCTGCTGATTCTGTCATTGGCGCTTTCTACGACGATTCGTCAGCCAGAGCAGAAAATGTTGCGTGAGGCGCATGGCTCCCTTTAA
SEQ ID NO:21(来自产甲酸草酸杆菌DSM 4420的草酸盐:甲酸盐反向转运蛋白(oxlT)；UniProt Accession Q51330；Of_oxlT)：
ATGAATAATCCACAAACAGGACAATCAACAGGCCTCTTGGGCAATCGTTGGTTCTACTTGGTATTAGCAGTTTTGCTGATGTGTATGATCTCGGGTGTCCAATATTCCTGGACACTGTACGCTAACCCGGTTAAAGACAACCTTGGCGTTTCTTTGGCTGCGGTTCAGACGGCTTTCACACTCTCTCAGGTCATTCAAGCTGGTTCTCAGCCTGGTGGTGGTTACTTCGTTGATAAATTCGGTCCAAGAATTCCATTGATGTTCGGTGGTGCGATGGTTCTCGCTGGCTGGACCTTCATGGGTATGGTTGACAGTGTTCCTGCTCTGTATGCTCTTTATACTCTGGCCGGTGCAGGTGTTGGTATCGTTTACGGTATCGCGATGAACACGGCTAACAGATGGTTCCCGGACAAACGCGGTCTGGCTTCCGGTTTCACCGCTGCCGGTTACGGTCTGGGTGTTCTGCCGTTCCTGCCACTGATCAGCTCCGTTCTGAAAGTTGAAGGTGTTGGCGCAGCATTCATGTACACCGGTTTGATCATGGGTATCCTGATTATCCTGATCGCTTTCGTTATCCGTTTCCCTGGCCAGCAAGGCGCCAAAAAACAAATCGTTGTTACCGACAAGGATTTCAATTCTGGCGAAATGCTGAGAACACCACAATTCTGGGTTCTGTGGACCGCATTCTTTTCCGTTAACTTTGGTGGTTTGCTGCTGGTTGCCAACAGCGTCCCTTACGGTCGCAGCCTCGGTCTTGCCGCAGGTGTGCTGACGATCGGTGTTTCGATCCAGAACCTGTTCAATGGTGGTTGCCGTCCTTTCTGGGGTTTCGTTTCCGATAAAATCGGCCGTTACAAAACCATGTCCGTCGTTTTCGGTATCAATGCTGTTGTTCTCGCACTTTTCCCGACGATTGCTGCCTTGGGCGATGTAGCCTTTATCGCCATGTTGGCAATCGCATTCTTCACATGGGGTGGTAGCTACGCTCTGTTCCCATCGACCAACAGCGATATTTTCGGTACGGCATACTCTGCCAGAAACTATGGTTTCTTCTGGGCTGCAAAAGCAACTGCCTCGATCTTCGGTGGTGGTCTGGGTGCTGCAATTGCAACCAACTTCGGATGGAATACCGCTTTCCTGATTACTGCGATTACTTCTTTCATCGCATTTGCTCTGGCTACCTTCGTTATTCCAAGAATGGGCCGTCCAGTCAAGAAAATGGTCAAATTGTCTCCAGAAGAAAAAGCTGTACATTAG
SEQ ID NO:22(来自动物双歧杆菌乳酸亚种DSM 10140的草酰辅酶A脱羧酶(OXC)；EC 4.1.1.8；UniProt Accession B9X297；Ba_oxc)：
ATGGTTGATGTAAGTGTAACTGCTACAAGCTCAGACCAGAATCTCACGGATTCTCCCCACTACCTCGCCGAAACGCTCATCAAGAACGGTGTCAAGCATATGTACGGCGTCGTCGGAATCCCGGTCACTGATTTCGCACGTATCGCACAGGGCATGGGCATCCGCTTCATCGGCATGCGCCATGAGGAGGACGCGGTGAACGCTGCCGCTGCCGAAGGATTCCTCACCGGTCGCCCAGCTGTGGCGCTCACCGTTTCCGCGCCGGGCTTCCTCAATGGTCTGGCACCGCTGCTTGAAGCCACCACGAACGGCTTCCCGGTCATCATGATCGGCGGTTCGTCCACTCGCCATGTCGTCGACATGCACGAAGGCGAATACGAAGGCCTCGACCAAATGAACTATGCGAAGCAGTTCTGCAAGGAATCGTTCCGCATCGACAAGATCGAAGACATTCCGCTTGCTGTGGCCCGCGCCATGCACATCGCATGCTCCGGCCGTCCGGGCGGTGTCTACATCGATTTCCCGGACGACGCCGTCGCCCAGACGCTCGACAAGGATGTCGCCGAGTCGCAGCTGTGGGTCGCGAACCAGCCGGCTCCGGCAATGCCGCCGGCGCAGTCCTCTGTGGATGAAGCGCTCAAGCTGCTCTCCGAGGCCAAGAACCCTCTCATGCTTGTGGGCAAGGGTGCGGCGCTGGCCCAGGCCGAGGACGAACTGCGTGAATTCGTCGAGAAGACCGACATGCCATTCCAGCCGATGTCGATGGCCAAGGGCGTCATTCCGGACGATGACCCACACTGCACGGCGAGCTGCCGCGGACTCGCGCTGCGCACCGCCGACGTCGTGCTGCTCGTCGGCGCTCGTCTGAACTGGATGCTCAATTTCGGCGAGGGCAAGGAATGGAACCCGAACGTCAAGTTCATCCAGATCGATATCGACCCGAACGAGATCGAGAACGCCCGTTCCATCGCATGCCCGGTGGTCGGCGACATCAAGTCCGCCATGCAGATGATCAATGCCGGACTCGAGAAGACGCCAGTGAAGGCGTCCGCGCAGTGGCTCGACATGCTCAAGGCCGACGCCGAGAAGAACGATGCCAAGTTCGCCGCCCGCGTGAACTCGAACACCGTGCCGATGGGTCACTACGACGCGCTCGGCGCCATCAAGAAGGTGTACGACCAGCACAAGGACATGATCCTGACCAACGAGGGCGCGAACACGCTCGACGATTGCCGCAACATCATCGACATCTACCAGCCGCGCCATCGTCTAGACTGCGGCACGTGGGGCGTTATGGGCTGCGCGGTCGGCTATTCAATCGGCGCCGCAGTGGCCACCGGCAAGCCTGTCCTGTACGTCGGCGGTGATTCCGGCTTCGGCTTTGACGGCATGGAAGTCGAGGTCGCCTGCCGCTACAATCTGCCGATCACCTTCGTCGTGCTCAACAACGGCGGCATCTACCGCGGCGATTTCGAGAATCTCGGCGACGACGGCGACCCGTCGCCGCTGACGCTGAGCTACGACGCCCACTACGAGCGCATGATCGAGGCGTTCGGCGGCAACGGCTATTACGCGACCACCCCGGCGGAAGTCGAGCAGATGGTCGGCGAGGCCGTCGCCTCCGGCAAGCCGAGCCTCGTGCACGTGCAGCTCGCCGATTATGCGGGCAAGGAGTCCGGGCACATCTCCAACCTGAACCCGAAGCCCGTCGTCGGCCCGCTCGCCACTTCCGAAATGACCGCGAACCCCTACCTCAAGGGCGCCCATATGTGA
SEQ ID NO:23(来自草酸盐贪铜菌ATCC 11883的草酰辅酶A脱羧酶(OXC)；EC4.1.1.8；Co_oxc)：
ATGGCAGAAGTCGGAAACGAGCTGCAGCGTCACGCCGCGGAAGAGCATCAGGCACAAACCGATGGTTTTCATCTGGTCATCGACGCGCTGAAGCTGAACGGCATCGAAAACATCTACGGCCTGCCCGGCATTCCGGTCACCGACCTGGCTCGGCTGGCGCAGGCAAACGGCATGCGCGTCATCAGCTTCCGCCACGAGCAGAACGCAGGCAACGCCGCAGCGATTGCCGGCTTTCTGACGCAAAAGCCAGGCGTTTGCCTGACCGTGTCTGCGCCAGGGTTCCTGAACGGGCTCACGGCGCTGGCGCATGCGACCACCAACTGCTTCCCGATGATCCTGATCAGCGGCTCGAGCGAGCGCGAGATCGTCGATCTGCAGCAGGGTGACTACGAAGAGATGGACCAGCTGGCCATCGCCCGTCCGCACGCCAAGGCTGCCTTCCGCGTGCTGCACGCCGAAGACATCGGCGTCGGGATCGCGCGTGCCATCCGTGCCGCCGTGTCGGGCCGCCCGGGCGGTGTGTACCTGGACCTGCCGGCCAAGCTGCTGGGCCAGTCGATGGAAGCCGAGAAGGGCCGCAAGTCGCTGATCAAGGTGGTGGATCCGGCCCCGCGCCAGCTGCCTGCTCCGGACTCGGTCGACCGTGCCGTCGCGCTGCTGAAGAGCGCCAAGCGCCCGCTGATCCTGGTAGGCAAGGGTGCTGCGTACGCACGCGCCGAAGCGGATATCCGCACGCTGGTCGAGAAGACGGGCATTCCGTACCTGCCGATGTCGATGGCCAAGGGCCTGTTGCCCGATACGCACCCGCAATCGGCCTCCGCCGCACGCTCGTATGTGCTGGCCGAAGCCGACGTGGTGCTGCTGGTCGGCGCCCGCCTGAACTGGCTGCTGTCGCACGGCAAGGGCAAGACCTGGGGCAAGCCGAAGCAATTCATCCAGATCGACATCGCGCCGACCGAGATGGACAGCAACGTCGCCATCGCCGCGCCGGTCGTGGGTGACATCGGTTCGTGCGTGTCGGCGATCCTCGACAAGGTCGGCGACGACTTTGCCAGGCCGGGCGCCGACTGGCTGAACGCGGTGGCTGACCGCAGGGACACCAACCTGGCGAAGATGGCCGAAACCCTGGCCAGGTCCAGGGACGCCTCGCCGATGAACTTCCACGGTGCGCTCGGCGTGCTGAAGGATGTGGTCAAGGCCAACCCGAACATCTCGTTCGTCAACGAAGGCGCCAACACGCTGGACTACGCCCGTGCCGTGATCGACATGTACGAGCCGCGCAAGCGCCTGGACGTAGGCACCTGGGGCGTGATGGGCGTGGGCATGGGCTACGCGGTCGCCGCCGCGGTCGAAACCGGCAAGCCGGTGCTGGCGCTGTGCGGTGACAGTGCGTTCGGCTTCTCGGGCATGGAAGTCGAAACGATCTGCCGCTACAACCTGCCGGTCTGCATCGTCATTTTCAACAACAACGGCGTCTACAAGGGGATCGACGTGAATCCGACCGGCGGCAGGGATCCGGCGGTCACGACGTTCGTCCCGGGCGCGCGCTACGACAAGATGATGGAAGCGTTCGGTGGTGTCGGTGCCAATGTCACCACGCCGGCGGAACTCGAAGCCGCGGTAAACGAAGCGCTGCGCTCGGGCAAGCCCACGCTGGTCAACGCCGTCATCGACCCGGCTGCCGGCACCGAAAGCGGTCGCCTGACCAATCTGAATCCGCAAAGCTCGGCCAAGAAGTAA
SEQ ID NO:24(来自大肠杆菌MG1655的草酰辅酶A脱羧酶(OXC)；EC 4.1.1.8；UniProt Accession P0AFI0；Ec_oxc)：
ATGTCAGATCAACTTCAAATGACAGATGGTATGCATATCATCGTTGAAGCATTAAAACAGAATAATATTGACACTATTTATGGTGTTGTAGGTATTCCTGTGACGGATATGGCACGCCATGCCCAGGCGGAAGGCATTCGTTATATTGGTTTTCGTCATGAGCAGTCGGCAGGCTATGCCGCTGCGGCAAGCGGTTTTCTTACCCAAAAACCGGGGATCTGCCTGACAGTTTCTGCGCCAGGATTCCTCAATGGTTTGACCGCATTGGCCAACGCAACGGTAAATGGTTTTCCGATGATCATGATTAGCGGCTCCAGCGACCGCGCGATCGTCGACCTACAGCAAGGTGATTATGAAGAGCTGGACCAAATGAATGCGGCAAAACCGTATGCCAAAGCAGCATTTCGCGTTAATCAGCCGCAGGATCTTGGCATTGCATTGGCACGCGCTATCCGGGTTTCTGTATCGGGTCGCCCTGGCGGAGTTTATCTTGATTTGCCAGCAAATGTCCTGGCCGCGACGATGGAAAAAGACGAAGCGTTAACCACGATTGTTAAAGTTGAAAATCCGTCGCCAGCATTATTGCCATGCCCGAAGTCAGTCACTAGCGCAATTTCGCTTTTAGCAAAAGCTGAACGGCCATTAATTATCCTTGGCAAAGGCGCGGCGTATTCACAAGCTGATGAACAGCTTCGTGAATTTATTGAAAGTGCTCAGATTCCATTCCTGCCAATGTCTATGGCGAAAGGGATCCTTGAAGATACGCATCCACTTTCTGCGGCAGCTGCGCGTTCGTTTGCCCTGGCAAATGCTGACGTTGTCATGCTTGTTGGTGCACGACTGAATTGGTTATTGGCACACGGTAAAAAAGGATGGGCGGCAGATACACAGTTTATTCAACTGGATATTGAACCGCAGGAAATTGACAGCAACCGCCCCATTGCTGTGCCAGTCGTTGGTGATATTGCATCCAGTATGCAAGGTATGCTGGCAGAACTGAAACAAAACACATTTACGACTCCACTGGTATGGCGCGATATTTTAAATATCCACAAGCAGCAAAATGCACAAAAAATGCATGAAAAATTAAGTACAGATACTCAACCATTAAATTACTTTAATGCATTAAGTGCTGTGCGCGACGTATTGCGCGAGAACCAGGATATTTATTTAGTTAATGAAGGTGCAAATACCCTGGATAATGCACGAAATATTATTGATATGTATAAACCACGTCGTCGTCTGGATTGTGGTACCTGGGGTGTCATGGGCATCGGTATGGGCTATGCCATCGGTGCTAGCGTGACTTCTGGTTCTCCGGTTGTCGCCATTGAAGGTGATAGTGCTTTTGGTTTCAGTGGGATGGAAATTGAAACGATTTGTCGATATAACCTGCCGGTGACGATCGTTATTTTTAATAATGGCGGCATCTACAGAGGAGATGGTGTTGATCTCAGTGGCGCTGGTGCACCATCACCAACGGATCTGTTGCACCATGCAAGGTATGACAAATTAATGGATGCGTTTCGTGGCGTTGGCTATAACGTCACCACGACAGATGAACTTCGTCATGCTTTAACCACCGGTATTCAGTCGCGCAAACCGACCATTATTAATGTGGTCATCGACCCTGCAGCAGGAACTGAAAGTGGCCATATTACCAAACTTAACCCAAAACAAGTCGCTGGTAATTAA
SEQ ID NO:25(来自产甲酸草酸杆菌DSM 4420的草酰辅酶A脱羧酶(OXC)；EC4.1.1.8；UniProt Accession P40149；Of_oxc)：
ATGAGTAACGACGACAATGTAGAGTTGACTGATGGCTTTCATGTTTTGATCGATGCCCTGAAAATGAATGACATCGATACCATGTATGGTGTTGTCGGCATTCCTATCACGAACCTGGCTCGTATGTGGCAAGATGACGGTCAGCGTTTTTACAGCTTCCGTCACGAACAACACGCAGGTTATGCAGCTTCTATCGCCGGTTACATCGAAGGAAAACCTGGCGTTTGCTTGACCGTTTCCGCCCCTGGCTTCCTGAACGGCGTGACTTCCCTGGCTCATGCAACCACCAACTGCTTCCCAATGATCCTGTTGAGCGGTTCCAGTGAACGTGAAATCGTCGATTTGCAACAGGGCGATTACGAAGAAATGGATCAGATGAATGTTGCACGTCCACACTGCAAAGCTTCTTTCCGTATCAACAGCATCAAAGACATTCCAATCGGTATCGCTCGTGCAGTTCGCACCGCTGTATCCGGACGTCCAGGTGGTGTTTACGTTGACTTGCCAGCAAAACTGTTCGGTCAGACCATTTCTGTAGAAGAAGCTAACAAACTGCTCTTCAAACCAATCGATCCAGCTCCGGCACAGATTCCTGCTGAAGATGCTATCGCTCGCGCTGCTGACCTGATCAAGAACGCCAAACGTCCAGTTATCATGCTGGGTAAAGGCGCTGCATACGCACAATGCGACGACGAAATCCGCGCACTGGTTGAAGAAACCGGCATCCCATTCCTGCCAATGGGTATGGCTAAAGGCCTGCTGCCTGACAACCATCCACAATCCGCTGCTGCAACCCGTGCTTTCGCACTGGCACAGTGTGACGTTTGCGTACTGATCGGCGCTCGTCTGAACTGGCTGATGCAGCACGGTAAAGGCAAAACCTGGGGCGACGAACTGAAGAAATACGTTCAGATCGACATCCAGGCTAACGAAATGGACAGCAACCAGCCTATCGCTGCACCAGTTGTTGGTGACATCAAGTCCGCCGTTTCCCTGCTCCGCAAAGCACTGAAAGGCGCTCCAAAAGCTGACGCTGAATGGACCGGCGCTCTGAAAGCCAAAGTTGACGGCAACAAAGCCAAACTGGCTGGCAAGATGACTGCCGAAACCCCATCCGGAATGATGAACTACTCCAATTCCCTGGGCGTTGTTCGTGACTTCATGCTGGCAAATCCGGATATTTCCCTGGTTAACGAAGGCGCTAATGCACTCGACAACACTCGTATGATTGTTGACATGCTGAAACCACGCAAACGTCTTGACTCCGGTACCTGGGGTGTTATGGGTATTGGTATGGGCTACTGCGTTGCTGCAGCTGCTGTTACCGGCAAACCGGTTATCGCTGTTGAAGGCGATAGCGCATTCGGTTTCTCCGGTATGGAACTGGAAACCATCTGCCGTTACAACCTGCCAGTTACCGTTATCATCATGAACAATGGTGGTATCTATAAAGGTAACGAAGCAGATCCACAACCAGGCGTTATCTCCTGTACCCGTCTGACCCGTGGTCGTTACGACATGATGATGGAAGCATTTGGCGGTAAAGGTTATGTTGCCAATACTCCAGCAGAACTGAAAGCTGCTCTGGAAGAAGCTGTTGCTTCCGGCAAACCATGCCTGATCAACGCGATGATCGATCCAGACGCTGGTGTCGAATCTGGCCGTATCAAGAGCCTGAACGTTGTAAGTAAAGTTGGCAAGAAATAG
SEQ ID NO:26(来自酿酒酵母S288C的草酰辅酶A脱羧酶(OXC)；EC 4.1.1.8；UniProt Accession P39994；Sc_YEL020C)：
ATGACCACGACCGCTACCCAACACTTCGCTCAACTTTTACAAAAATATGGCATCGACACAGTCTTTGGAATTGTGGGCATCCCTATTGTCCAACTAGCGGATACGATGGTTGCCAACGGTATCAAATTCATTCCATGCAGGAACGAACAGGCTGCCTCATATGCAGCCTCTGCTTATGGTTATATTAGTGATAAGCCCGGCGTGTTACTTATTGTCGGGGGCCCCGGTTTAATCCACGCACTGGCTGGTATATACAACTCAATGAGTAATAGGTGGCCTCTTCTAGTAATTGCAGGGAGTTCTTCTCAAAGTGATATACATAAAGGCGGGTTTCAAGAGTTGGATCAAGTTAGTCTTTTGTCCCCATTTTTGAAGTTTACCGGAAAGTTAACCCCTGACAATATTGACATGATTACTCAGAAGGCTTTGAATTACTGTATACAAGGCACTGCAGGGGTTTCTTACATAGACGTCCCTGCAGATTTTATTGAGTATGAAAAACCCTTAGAAGGAAACGACCGTACAGGAAATGAATTACCGATGATATTAACTCCAAACATATGTGGCCCTGATCCGTCGAAGATCAAAAAAGTCGTACAGCTTATTTTGCAACATAAAAATAAAAATATTCTGATTGTTATTGGAAAGGGTGCGGTAAAAAACTCACACGAAATCCGCAGACTAGTAAATACGTTCAATCTACCATTTTTACCCACCCCAATGGCTAAGGGAATTGTCCCAGACTCTTCTCCACTGAACGTTTCATCTGCAAGATCTCAAGCATTGAAAATAGCGGATATTGTTCTTGTTCTCGGGGCAAGATTAAACTGGATATTGCATTTTGGTACTTCACCCAAATGGAACTCAGAATCCATATTCATCCAGTTCGATTCCAATCCCGAAACCTTGGGGGATAACAATGTCTCACCTGGTGCTGACCTTTCCATATGGGGAGATATAGGCTTGAGCGTAACCGCTTTAGTTGAGGAATTGACTCGCCAAGATTCCTGCTGGAAGTATAGTGGTGTTAAGCAAGAAATACGAGAGAAAATTCAACTGAATCAAACTCGTCTATTGAGGAAAGAGAAGACTAGAGGGGCACAATTGAATTACAACCAGGTTTATGGAACATTGAGGCCTCTTATCGATGATTACAGGACAATACTTGTGACGGAGGGAGCAAATACTATGGATATTGCGCGTATTTCATTCCCTACAGACGCTCCAAGGCGCCGTTTAGACGCAGGGACCAACGCGACTATGGGGATTGGGCTCGGATATGCTCTTGCATGCAAGGCATCTCATCCGGAACTCGATGTGGTGCTGATTCAGGGTGATTCCGCATTTGGATTCTCTGCCATGGAAATTGAAACGGCAGTAAGGTGTCAGCTGGCATTGGTCATCGTTGTGATGAACAATAGCGGTATTTACCATGGAGAAAAGGATATAGAGGGTGATTTACCTCCAACGGCACTAAGCAAGAACTGCCGTTACGATCTCGTAGGCAAAGGACTGGGTGCCAATGATTTTTTTGTCAACACAATAAGCGAACTGAGCAGATGCTTCCAACAAGCCGTGCAGCTTTCTCGGACTAAAAGAGAAACAAGTGTCATCAACGTTATCATTGAGCCTGGCGAACAAAAGCAAATTGCCTTTGCCTGGCAGAATAAACCGCGTTTATGA
SEQ ID NO:27(来自扭脱甲基杆菌AM1/DSM 1338的草酰辅酶A还原酶/乙醛酸盐:NADP+氧化还原酶(OXR)；EC 1.2.1.17；UniProt Accession C5APN9；Me_PanE)：
ATGTCAATTGCCATTGTTGGTGCCGGTGCAATTGGAGGATATTTAGGTGTCAGATTAGCTGAAGCTGGTGAGGATGTTACATTCATAGCTAGATCTAATGCTGCAGCCATACAAGCAGATGGAATGAGATTGATTGAAGAGGATGGGACCGAAATTCACTCTAAATCTGTCAAAGCCACTCGTTCAATGCAAGAAGCAGGAGTTCACGAGGTTGTTTTATTAACAGTTAAGGCACACCAAGTTGGTCCTATAGCTGCCGATTTACATCACTTGATAGGTCCAGACACCGTAGTCGTCACTATGCAGAATGGTATTCCTTGGTGGTACTTTCTAGGTGGATACTCTGGAGATCATGCAGGAACAAGGTTGGAATCAGCTGATCCAGGAGGTTTAATTGCTGATCATTTAGACCCTAAGCATGTCATTGGTTCTGTTGTTTACCCTGCAACTGTATTGACAGACCCAGGTACTGTTAAAGTTATCGAGGGTAATAGGTTTGGTTTGGGAGAATTGGATGGTTCAAAATCTGAAAGAGTTTTAGCCTTATCTCAGAGGTTAGCAAGGGCCGGTTTTAGAGCCCCAGTCACTTCAGACATTAGAGCAGAGATCTGGTTGAAGTTGTGGGGAAATTTATCTTTCAATCCCATTAGCGCCTTAACACATGCAACCTTGGAGGACATCTGTAGATTTCCTGATACTAGGGCTATCGCCGCAGAGATGATGAGAGAGGCCGAAGTCATCGCCAATAAATTGGGTGTAACATTTAGATTAGGAATAGATAAGAGAATAGCTGGAGCTGAAAAGGTCGGTCCACACAAGACCTCTATGTTGCAAGACGTAGAAGCTGGTAGACCAATTGAGTTGGAAGCCTTAGTCGGATCTGTAATCGAATTAGGAAGGTTAACTGGTACTCCTACTCCACATATTGATACAGTCTTTGCTTTGATGAGATTATTGGCCCAATCTTTAGAAAGAGCACAAGGTAGATTGGCTATTCAGGGAGCATAA
SEQ ID NO:28(来自草酸盐贪铜菌ATCC 11883的假定琥珀酰辅酶A:草酸辅酶A转移酶(SCOCT)；EC 2.8.3.2；Co_ACOCT)：
ATGACGCAATCCAACCTGCCCGATTTGCCCGACCTGCCGCTGACCGGCCTGCGCGTGATCGATTTCTCGCGCGTGCTGGCCGGCCCCTATTGCACCGCGCTGCTCGGCGACCTCGGCGCCGAGGTGATCAAGGTCGAGCCGCCCGGCGGCGACGACTATCGCGCGGTGGGTCCCTTCGCCGGCGGCAAGAGCGGCCTGTTCTGCGCGATGAACCGCAACAAGCAGAGCATCGTGATCGACCTGAAGACGGAGGACGGCCTGGCTGTCGCCCGCGCGCTGTGCCGCGGTGCCGACGTGGTGGTGGAAAACTTCCGCCCCGGCGTGGCCGACAAGCTTGGCATCGGCTACGCCGCGCTGCGCGAACTGAACCCCTCGCTGGTCTATGCCAGCGTGTCGGGCTTCGGCCAGACCGGCCCGGAATCGCACCGCCCGGCCTACGACATCATCCTGCAGGCGATGTGCGGGCTGATGGACGCCACCGGCGCGCCCGACGGCGCGCCGACCATGCTTGGCGAAGCCGTGTCCGATGCGGTCAGCGGCCTGTTCGCCTCGTGGGGCGTGCTGGCCGCGCTGCTGGCGCGCGAGAAGACAGGGCGCGGCACGCATGTCGATGTGTCGATGTTCGATGCCACGCTCAGCCTCAGCGCCACGCTGGTCGCGCGCTACGCGGCCACCGGGCTGGCGCCGCGGCGCGTGGGCAACCGCCACCCGTCGTCGGCGCCGTTTGGCGCCTACCGCGCCGCGGATGGCTTCTACGTGGTCGCGGTGCTGAACAACAAGCTGTTCCAGGCGCTTGCCGACGCCATCGGCCGGCCCGCGATGGCGCACGACCCGCGCTTTGCCGATGACGAAACGCGCTGCCGCTTCGAGTCCGACCTGCGCGCCATGCTGGAAAACTGGTCGGCCACGCGCACGGTGGCGGAGGTGAACGCAGCGCTCGGCGCCGCCGGCATCCCGGTCGCACCGATCCGCAATGTCAAGGAAGCGCTGGAAAGCGAGCACGCGGCGCATCGCCGCCTGCTGACCGAGGTGCCCGACACGGACGGCGGCACCGTGCGCCTGCCGTCGCAGCCGGTCAAGTTCTCCGCGTACGGCACCAACCGCGTGACGCCCGCGCCGGCACTGGGCCAGCACACCGGGGCCATCCTGGCCGCGCCCGATTTCAGTAATTTCAATAAGGAGAAGCAACATGCATAA
SEQ ID NO:29(来自草酸盐贪铜菌ATCC 11883的假定琥珀酰辅酶A:草酸辅酶A转移酶(SCOCT)；EC 2.8.3.2；Co_CaiB)：
ATGGGAGCCTTAAGCCATCTCCGCGTCCTCGACCTGACCCGTGTCCTTGCCGGGCCCTGGTGCGCCCAGAACCTTGCCGACTTCGGCGCCGATGTGATCAAGATCGAGCGCCCCGGCGCCGGCGACGACACCCGCACCTGGGGCCCGCCCTGGCTCAAAGACGAAGACGGCCGCGACACCGCCGAGGCCGCCTACTACCTGGCCGCCAACCGCAACAAGCGCTCGGTCACCTGCGACATCAGCACGCCCGAAGGCCAGCAGATCGTGCGCGACCTCGCCGCGCAGAGCGACGTGGTGCTGGAGAACTACAAGGTCGGCCAACTCAAGAAATACGGGCTGGACTACGAGTCGCTCAGGCAGGTCAAGCCCGACCTGATCTACTGCTCGGTCACCGGCTTCGGCCAGACCGGCCCGTACGCCGCGCGCCCCGGCTATGACTTCATCATCCAGGGCATGGGCGGCTTCATGAGCCTGACCGGCGAGCGCGACGACCTGCCCGGCGGCGGCCCGCAGAAGGCCGGCGTGGCGATTTCCGACCTGATGACGGGCCAGTACGCCACCATCGCCGTGCTGGCGGCGCTGGCGCATCGCGAGCGCACCGGCGAAGGCCAGTACATCGACATGGCGCTGCTGGACGTGCAGGTGGCCATGCTGGCCAACATGAACACCAACTACCTGGCCAGCGGCGAGGCGCCGCGCCGCTGGGGCAATGCGCACCCCAACATCGTGCCCTACCAGACCTTCCAGGCCGCCGACGGCTGGATCATCGTCGCGGTGGGCAACGACGGGCAGTTCCGCAAGTTCGTCACCGACGGCGGCATGCCCGAACTGGCCGACGATCCGCGCTTTGCCAAAAACCCGCAGCGCGTGGCCAACCGCGACGTGCTGGTGCCGATCCTGGCCGAGATGGTGCGCCCGCGCACCCGCGCGCAGTGGATCCGCGACCTGGAAGCCGCGGGCGTGCCGTGCGGCCCGATCAACACGCTCGACGATGTGTTCGAGGACGACCAGGTCAAGGCGCGCGGCCTGCGCGTGGACCTGCCGCACCCCAGCGCCGGCGAGGTCAAGCTGGTCGCCAGCCCGATCAAGATGAGCGCGACGCCGCCGCAGGCGCTGCGCCACCCGCCGCTGCTGGGCGAGCACACCGACACGGTGCTGGCCGAAACCCTGGGCTACAGCGCCGGGCAGATCGCGGCACTTCGCGCAAAAGGTGTGCTGTAA
SEQ ID NO:30(来自草酸盐贪铜菌ATCC 11883的假定琥珀酰辅酶A:草酸辅酶A转移酶(SCOCT)；EC 2.8.3.2；Co_SCACT)：
ATGGACAAGGAACGCATTCGCCTGGCGAGGCTGCATGACAAGGTAGTCTCGGCCGACGAGGCCGCCGCGCTGATCCGCGACGGCATGACCGTAGGCATGAGCGGTTTCACCCGCGCGGGCGACTGCAAGGAAGTCCCGTTCGCGCTGGCGCGGCGCGCGGCGACCGAGCCGCTGCGCATCACCCTGATGACGGGCGCCTCGCTCGGCAACGACATCGACCGCGTCCTGGCCGAGGCCGACGTGATCGCGCGCCGCCTGCCGTTCCAGTCCGATGGCACGCTGCGCCGCAAGATCAACGCCGGCGAGGTGATGTTCATCGACCAGCACCTGTCCGAAACCGTCGAGCAGCTGCGTTCCGGCCAGATCGCGCCGGTGGACGTGGCGGTGGTCGAGGCCGTGGCGATTACCGAGCAGGGCGGCATCATCCCCAGCACCTCGGTGGGCAATTCGGCAAGCTTTGCCATGCTGGCGCGCAAGGTGATCGTCGAGATCAACATGAACATGCCGCTGGAGCTGGAAGGGCTGCACGACATCTATTTCCCGGTCCAGCGCCCGTACCGCCAGCCGATCCCGCTGATCGCGCCGGAACAGCGCATCGGACTCCCGTATATTCCGATCGATCCGGAGAAGATTGCCGCCATCGTCATCACCGCCAAGGACGACAGCCCGTCCAATGCGCTGCCGCCGGACGACGAAACCCGCAGCATCGCCGGCCACCTCAATGACTTCCTGCTGCGCGAAGTGCGCGCGGGCAAGCTGTCGCCGTCGCTGCAGCCGCTGCAGGCCGGCATCGGCACCATTGCCAATGCCGTGCTGCACGGGCTGGTCGAGTCGCCGTTCCGCGACCTGAAGATGTATTCCGAGGTGCTGCAGGACAGCACCATCGAGTTGCTCGATGCGGGCCGGCTGTCGTTCGCGTCGGCGTCGTCGGTGACGCTGACGCGCGAGGTCTACCAGCGCTTCCTGTCGAACCTGGACCGCTACCGTTCGCGCCTGCTGCTGCGCCCGCAGGAGATCAGCAACCATCCCGAGATCCTGCGCCGGCTGGGGCTGATCACCATCAACACCGCGCTGGAGTGCGACATCTACGGCAACGTCAACTCCACGCATGTGGGTGGCACGCACATGATGAACGGCATCGGCGGCTCCGGCGACTTTGCGCGCAATGCGCACGTGTCGGTGTTCGTGACCAAGTCGGTGGCCAAGGGCGGCGAAGTCTCGAGCATCGTGCCGATGGTGGCGCACGTGGACCACACCGAGCACGATGTCGACATCATCGTCACCGAGCACGGCCTGGCCGACCTGCGCGGCCTGGCACCGCGCGAGCGCGCGCGCACCGTCATCGCCAATTGCGCCGATCCGCAATACCGCGAGTTGCTGGGCGACTATTTCCGCCGCGCCAGCGCCCACGGCGGCCAGACGCCGCACCTGCTGGAAGAGGCGTTGTCCTGGCATGTCGGCTTGCGCGATCGCGGCACCATGCGGTCCGCACAGCCGGCGCAGGCCCTGGCTGCCTGA
SEQ ID NO:31(来自草酸盐贪铜菌ATCC 11883的假定琥珀酰辅酶A:草酸辅酶A转移酶(SCOCT)；EC 2.8.3.2；Co_SCMCT)：
ATGGTCAACGAAGCGAACAAGGAAAAGATGCCGCTGCAAGGCGTGCGCGTGTTGGAGCTGGGATCGCTGATCGCCGGCCCGTATGCCGGCGGCCTGCTCGCCCAGTTCGGCGCCGAGGTGCTGAAGATCGAAGCGCCGGGCGAAGGCGATCCGCTGCGCAAATGGCGCAAGCTGTACGAGGGCACCTCGCTGTGGTGGTACAGCCAGAGCCGCAACAAGAAGTCGCTGACGCTGGACCTGCGCAGCGCGCAAGGCCAGGACATCGTGCGCAAGCTGGTGGCCGGCGCCGATATCGTGATCGAGAACTTCCGCCCCGGCACGCTGGAGAAATGGGGCCTGGGCTGGGAAGACCTGCGCAAGGTGAATCCGGCCCTGGTCATGGTGCGCATTTCCGGCTATGGCCAGACCGGCCCCTACAAGGACCGCCCGGGGTTTGCCGCGATCGCGGAATCGATGGGCGGGCTGCGCTATGTCAGCGGCTACCCCGACCGGCCGCCGGTGCGGTCCGGCGTCAGCATCGGCGACACGCTGGCGTCGCTGTACGGCGTGATCGGCGCGCTGCTGGCCATGCACCATCTGCGCAACAACGATGGCGAAGGGCAGTTTGTGGACGTGGCCCTGTACGAGTCGGTCTTTGCCGTGATGGAAAGCCTGGTGCCGGAGTTCGACAAGTTCGGCCATGTGCGCGAGCGTTCCGGCGCCAGCCTCCCCGGCATTTCGCCGTCGAACACATATCCCTGCCAGGATGGACAGTACGTGATCATCGCGGGCAATGGCGACGCCATCTTCCGGCGCTTCATGACGGCGATCGGCCGCGACGACCTCGGCCAGGATCCGCGCCTGGCTCACAACGATGGCCGCGTGCAGCACAACGCCATGCTCGACGACGCCATCGCCGCATGGACCGCCGCGCACGACCTCGACACCGTGCTGGCGACGCTGGAAGCCGCGGCGGTGCCTTGCGGGCGCATCTACACCGCGCAGGACATCTGCAGCGACCCCCACTACAGTGCGCGCGGCATGATCGAGCCGCACCAGCTTCCGGACGGCTCGCCGCTGGCCCTGTCGGGCATCGTGCCCAGGCTCAGTGAAACCCCCGGGCGTACCAACTGGATCGGCCCGGCGCTGGGCGAGCACACCGAAGAGATCCTGGCGGGCATCGGCATTGCCGGCGCCGAATTCCGCGCGCTGCGCGAGGCCGGCGTGGTCTGA
SEQ ID NO:32(来自醋化醋杆菌菌株1023的乙酰辅酶A:草酸辅酶A转移酶(ACOCT)；EC 2.8.3.19；UniProt Accession A9X6P9；Aa_CoAT3)：
MTETTPAKPKGPFDGLLVIDLTHVLNGPFGTTILTDLGARTIKIEPPGHGDDTRTYGPYVGDQSLYFSFVNRGKESIVLNLKDEGDRAIFLEMVRKADVLAENFRPGVMDRLGFNYEELAKINPRLIYASSSGFGQTGPLAHYPAYDTIVQAMSGIMMATGFPDGPPTRVGGTSLSDLCGGVFMFCGIASALYARERTGKGAHIDVSMFDGTLAFLQHALMCWSATGKAPARIGNRHPYMAPFDVFQAQDKPFVICCGNDHLFKALCDVIGAPELATDPRFVENHDRMANNDALKAALEKALSKQPAAHWLDVIHKAGVPVGPLLDVAEAANLPQTAARNMLIKSGGVMMPGNPVKISGYDDPHERPGAPKLDEQGAALRKEFAAPEAK
SEQ ID NO:33(来自大肠杆菌MG1655的乙酰辅酶A:草酸辅酶A转移酶(ACOCT)；EC2.8.3.19；UniProt Accession P76518；Ec_yfdE)：
MTNNESKGPFEGLLVIDMTHVLNGPFGTQLLCNMGARVIKVEPPGHGDDTRTFGPYVDGQSLYYSFINHGKESVVLDLKNDHDKSIFINMLKQADVLAENFRPGTMEKLGFSWETLQEINPRLIYASSSGFGHTGPLKDAPAYDTIIQAMSGIMMETGYPDAPPVRVGTSLADLCGGVYLFSGIVSALYGREKSQRGAHVDIAMFDATLSFLEHGLMAYIATGKSPQRLGNRHPYMAPFDVFNTQDKPITICCGNDKLFSALCQALELTELVNDPRFSSNILRVQNQAILKQYIERTLKTQAAEVWLARIHEVGVPVAPLLSVAEAIKLPQTQARNMLIEAGGIMMPGNPIKISGCADPHVMPGAATLDQHGEQIRQEFSS
SEQ ID NO:34(来自酿酒酵母S288C的甲酸脱氢酶；EC 1.2.1.2；UniProtAccession Q08911；Sc_FDH1)：
MSKGKVLLVLYEGGKHAEEQEKLLGCIENELGIRNFIEEQGYELVTTIDKDPEPTSTVDRELKDAEIVITTPFFPAYISRNRIAEAPNLKLCVTAGVGSDHVDLEAANERKITVTEVTGSNVVSVAEHVMATILVLIRNYNGGHQQAINGEWDIAGVAKNEYDLEDKIISTVGAGRIGYRVLERLVAFNPKKLLYYDYQELPAEAINRLNEASKLFNGRGDIVQRVEKLEDMVAQSDVVTINCPLHKDSRGLFNKKLISHMKDGAYLVNTARGAICVAEDVAEAVKSGKLAGYGGDVWDKQPAPKDHPWRTMDNKDHVGNAMTVHISGTSLDAQKRYAQGVKNILNSYFSKKFDYRPQDIIVQNGSYATRAYGQKK
SEQ ID NO:35(来自动物双歧杆菌乳酸亚种DSM 10140的甲酰辅酶A:草酸辅酶A转移酶(FCOCT)；EC 2.8.3.16；UniProt Accession B9X295；Ba_frc)：
MADKSTAPLAGIKVIDWTQVQSGPSCTQILAWLGAEVIKLEKVHGGDPTRNEMNDVDGSYSLYFLQLNANKKSITLDMKDPEGKKILTDLLKDADVFVENIGPGDVEKLGFGWDEVHKINPKLIMASLKGFNQGSRFEHVKAFEPVAQCAGGAASTTGWWEGDKNIPTQSGAALGDSNTGMHLTIAILTALLQRERTGEGVFVYQSMQNAVLNLCRIKLRDQLILDHLHQLSYYDCYPGYKFGKAIPRAANAEGGLVLGWCYRAKGWETDPNAYVYIVIQQSQKGFENFCNAMGFQDWLTDPKFSTPNARDEHKQEVYKRVEEYTMQYDKYTLTKELGAKGVPVGPVLDWNELENDPDLNEDGTLITIDQGDARGKFKTIGLPFTMSNYAPDYQRAPKLGENNEEILKSLGYTDEQIADLATKGVIGSNDGVKADLTAAPAQA
SEQ ID NO:36(来自草酸盐贪铜菌ATCC 11883的甲酰辅酶A:草酸辅酶A转移酶(FCOCT)；EC 2.8.3.16；Co_frc1)：
MPAQHTHATPAGPLDGIRVLDLTAVVLGPLATQVLADFGADVIKIEGPEGDLMRANGVSQHAGMSSIYLALNRNKRSVVLDLKIPEGAEALRALIAGADVLVHNMRVAAIERLGFGYEAVARINPRIVYCVATGFGQDGPHRDKPAFDDIIQAGCGLVALGAGNGERPEYVPSLIADKTTGLALANAVLAALLHRERHGVGQSVEVPMLETMAAFVMTEHLGGLTFEPAPAGAGYARLLQGGRRPAPTRDGWICALPYTERHWHAFFRAVGRDDLADRYEVGDRAQRNANIRALYGHLAELTPERSTAEWMALFEALDIPATPIYDLDALVDHPHLRAVGLFQATQHPTEGPLREVRPAARFSATPLSLRRHAPALGEHTAEVLQEAGIALPAAHAGTPAGAQE
SEQ ID NO:37(来自草酸盐贪铜菌ATCC 11883的甲酰辅酶A:草酸辅酶A转移酶(FCOCT)；EC 2.8.3.16；Co_frc2)：
MNLPLNGIKIIDFTHVQAGPACTQLLAWFGADVIKVERPGSGDVTRTQLRDIPDVDALYFTMLNSNKRSLTLDTKKPEGKKILEQLIRESDVLVENFGPGALDRMGFSWERINELNPKMIVASVKGFSDGHHYEDLKVYENVAQCAGGAASTTGFWDGPPTVSAAALGDSNTGMHLAIGILTALIGRDKTGKGQKVAVSMQDAVLNLCRVKLRDQQRLDRLGFLEEYPQYPHGSFSDVVPRGGNAGGGGQPGWVLKCKGWETDPNAYIYFTIQGHAWEPICKALGKPEWIDDPNYATAKARQPHIFDIFNTIEEWLADKTKYEAVDILRKHDIPCSPVLSMKEIAADPSLRASGSITEVPHKERGTYLTVGSPIKFSDLKPEITGSPLLGEHSEEVLAGLGYGTDDIKRLRESQVI
SEQ ID NO:38(来自大肠杆菌MG1655的甲酰辅酶A:草酸辅酶A转移酶(FCOCT)；EC2.8.3.16；UniProt Accession P69902；Ec_frc)：
MSTPLQGIKVLDFTGVQSGPSCTQMLAWFGADVIKIERPGVGDVTRHQLRDIPDIDALYFTMLNSNKRSIELNTKTAEGKEVMEKLIREADILVENFHPGAIDHMGFTWEHIQEINPRLIFGSIKGFDECSPYVNVKAYENVAQAAGGAASTTGFWDGPPLVSAAALGDSNTGMHLLIGLLAALLHREKTGRGQRVTMSMQDAVLNLCRVKLRDQQRLDKLGYLEEYPQYPNGTFGDAVPRGGNAGGGGQPGWILKCKGWETDPNAYIYFTIQEQNWENTCKAIGKPEWITDPAYSTAHARQPHIFDIFAEIEKYTVTIDKHEAVAYLTQFDIPCAPVLSMKEISLDPSLRQSGSVVEVEQPLRGKYLTVGCPMKFSAFTPDIKAAPLLGEHTAAVLQELGYSDDEIAAMKQNHAI
SEQ ID NO:39(来自产甲酸草酸杆菌DSM 4420的甲酰辅酶A:草酸辅酶A转移酶(FCOCT)；EC 2.8.3.16；UniProt Accession O06644；Of_frc)：
MTKPLDGINVLDFTHVQAGPACTQMMGFLGANVIKIERRGSGDMTRGWLQDKPNVDSLYFTMFNCNKRSIELDMKTPEGKELLEQMIKKADVMVENFGPGALDRMGFTWEYIQELNPRVILASVKGYAEGHANEHLKVYENVAQCSGGAAATTGFWDGPPTVSGAALGDSNSGMHLMIGILAALEMRHKTGRGQKVAVAMQDAVLNLVRIKLRDQQRLERTGILAEYPQAQPNFAFDRDGNPLSFDNITSVPRGGNAGGGGQPGWMLKCKGWETDADSYVYFTIAANMWPQICDMIDKPEWKDDPAYNTFEGRVDKLMDIFSFIETKFADKDKFEVTEWAAQYGIPCGPVMSMKELAHDPSLQKVGTVVEVVDEIRGNHLTVGAPFKFSGFQPEITRAPLLGEHTDEVLKELGLDDAKIKELHAKQVV
SEQ ID NO:40(来自Bacteroides subtilis 168的草酸脱羧酶(OXDC)；EC4.1.1.2；UniProt Accession O34714；Bs_oxdC)：
MKKQNDIPQPIRGDKGATVKIPRNIERDRQNPDMLVPPETDHGTVSNMKFSFSDTHNRLEKGGYAREVTVRELPISENLASVNMRLKPGAIRELHWHKEAEWAYMIYGSARVTIVDEKGRSFIDDVGEGDLWYFPSGLPHSIQALEEGAEFLLVFDDGSFSENSTFQLTDWLAHTPKEVIAANFGVTKEEISNLPGKEKYIFENQLPGSLKDDIVEGPNGEVPYPFTYRLLEQEPIESEGGKVYIADSTNFKVSKTIASALVTVEPGAMRELHWHPNTHEWQYYISGKARMTVFASDGHARTFNYQAGDVGYVPFAMGHYVENIGDEPLVFLEIFKDDHYADVSLNQWLAMLPETFVQAHLDLGKDFTDVLSKEKHPVVKKKCSK
SEQ ID NO:41(来自大麦的草酸氧化酶(OXO)；EC 1.2.3.4；UniProt AccessionP45850；Hv_OxOx)：
MSDPDPLQDFCVADLDGKAVSVNGHTCKPMSEAGDDFLFSSKLTKAGNTSTPNGSAVTELDVAEWPGTNTLGVSMNRVDFAPGGTNPPHIHPRATEIGMVMKGELLVGILGSLDSGNKLYSRVVRAGETFVIPRGLMHFQFNVGKTEAYMVVSFNSQNPGIVFVPLTLFGSDPPIPTPVLTKALRVEAGVVELLKSKFAGGS
SEQ ID NO:42(来自热醋穆尔氏菌ATCC 39073的草酸氧化还原酶(OOR)α亚基；EC1.2.7.10；UniProt Accession Q2RI41；Mt_OORA！)：
MGKVRNISGCVAVAHGVRLADVDVICSYPIRPYTGIMSELARMVADGELDAEFVHGEGEHAQLSVVYGASAAGARVFTGSSGVGVTYAMEVYSPISGERLPVQMAIADRTLDPPGDFGEEHTDAECCRDQGWIQGWASTPQEALDNTLIYYRVGEDQRVLLPQYACLDGYFVSHILGPVDIPDEAQVKEFLPPYKNHHVLDPRKPQIIGPQIEPAMGPPLQYQRYQAVKGVHKVLEEACDEFARIFGRKYDPYLDEYLTDDAEVIIFGQGAHMETAKAVARRLRNLGEKVGVARLRTFRPFPTEQIKERLSKFKAIGVLDVSANFGISCSGGVLLSELRAALYDYGDKVKTVGFVAGLGGEVVTHDEFYRMFQKLKEIAKTGKVEQTSYWIPFEL
SEQ ID NO:43(来自热醋穆尔氏菌ATCC 39073的草酸氧化还原酶(OOR)β亚基；EC1.2.7.10；UniProt Accession Q2RI42；Mt_OORB)：
MMDRIASIKKAPDEEYYVPGHRTCAGCGPALTYRLVAKAAGPNTIFIGPTGCMYVANTSYGCGPWRVPWIHAQITNGGAVASGIEAAYKAMIRKKKTDAEFPNIIVMAGDGGAVDIGLQALSAMLYRGHDVLFICYDNESYANTGIQTSPTTPYGANTTFTPPGEVVPEGKKLFPKDNPKVIAHGHPELKYVATASIGWPVDLMNKVRKGLNQEGPAYIHIHAPCPKGWQFPADKTIEMAKLAVQTGMFQLYEYENGEYKLSVKVDKRKPVSEYMKLQKRFAHLKPEHIAKMQAFVDARCAEVGITVPVVASNA
SEQ ID NO:44(来自热醋穆尔氏菌ATCC 39073的草酸氧化还原酶(OOR)δ亚基；EC1.2.7.10；UniProt Accession Q2RI40；Mt_OORD)：
MSTKDLFAEPNLKQITVWARGVVMNKDARDIVVALTEAAAKEGKYVQAWENYVDLPDRIYVPVRAYARISSDPIESKYIYENETPDIVVLVEESLIKGVPILKGIRPGSTLVVNTKRSIDTILEFLGDTGNLAQIVTVDANSMAEAVMTLSGAEGATDATGIGAGIAAPIAGAVVKATGIVDVENLAAVVKNPAAMRRGYAEAQVRQLPPHEAVEEAAVSATELLRQMPFAGTVPSPVTENEGMVTGNWRIQRPIIDREACTECYTCWIYCPDSCITRTEEGPVFNMKYCKGCGLCTAVCPSGALTNVPELDFKD
SEQ ID NO:45(来自拟南芥col-1的草酸盐--辅酶A连接酶/草酰辅酶A合成酶(OXS)；EC 6.2.1.8；UniProt Accession Q9SMT7；At_AAE3)：
MDSDTLSGLLENVAKKFPDRRALSVSGKFNLTHARLHDLIERAASRLVSDAGIKPGDVVALTFPNTVEFVIMFLAVIRARATAAPLNAAYTAEEFEFYLSDSDSKLLLTSKEGNAPAQEAASKLKISHVTATLLDAGSDLVLSVADSDSVVDSATELVNHPDDGALFLHTSGTTSRPKGVPLTQLNLASSVKNIKAVYKLTESDSTVIVLPLFHVHGLLAGLLSSLGAGAAVTLPAAGRFSATTFWPDMKKYNATWYTAVPTIHQIILDRHASHPETEYPKLRFIRSCSASLAPVILSRLEEAFGAPVLEAYAMTEATHLMSSNPLPEEGPHKPGSVGKPVGQEMAILNEKGEIQEPNNKGEVCIRGPNVTKGYKNNPEANKAGFEFGWFHTGDIGYFDTDGYLHLVGRIKELINRGGEKISPIEVDAVLLTHPDVSQGVAFGVPDEKYGEEINCAVIPREGTTVTEEDIKAFCKKNLAAFKVPKRVFITDNLPKTASGKIQRRIVAQHFLEKP
SEQ ID NO:46(来自扭脱甲基杆菌AM1/DSM 1338的草酸盐--辅酶A连接酶/草酰辅酶A合成酶(OXS)；EC 6.2.1.8；UniProt Accession C5APP0；Me_oxs)：
MTMLLPEPSALLPTDSKEASVPATTLHELIQAGADAAPALSSPGGVPLTFQALRALTERTVADLNARGIGRGDRVAIVLPNGPEMAAAFIAVAAGTTSAPLNPSYKADEFEFYMSDLGAKLLLVAEGSETPAVAVAEKLGVSVARLRPTPDEGAGSFTLHFASESTGPTEKSGPAGSDDIALVLHTSGTTSRPKIVPLTQANVCASARNIRTALAFGPEDRGLNIMPLFHIHGLIAGILAPLSVGGQVSCTPGFNALKFFGWMDEVNPTWYTGVPTMHQAILGRAARNKEIIARNPLRFIRSSSSSLPPQVMKELEETFGAPVIEAYGMTEAAHQMASNPLPPKPHYAGSVGLAAGPEIAVVDLDGEPLPAGETGEIVIRGDNVMKGYENNEKANAEAFTKQGWFRTGDQGVLSPEGYLSITGRLKEIINRGGEKISPREVDEILMDHPAVSQCVTFAVPHDKLGEDVAAAIVLREGVEAVEKDIRSFASERLAAFKVPAKILILDEIPKGATGKLQRIGLAQKLGLV
SEQ ID NO:47(来自酿酒酵母S288C的草酸盐--辅酶A连接酶/草酰辅酶A合成酶(OXS)；EC 6.2.1.8；UniProt Accession P38137；Sc_YBR222C)：
MTSAATVTASFNDTFSVSDNVAVIVPETDTQVTYRDLSHMVGHFQTMFTNPNSPLYGAVFRQDTVAISMRNGLEFIVAFLGATMDAKIGAPLNPNYKEKEFNFYLNDLKSKAICVPKGTTKLQSSEILKSASTFGCFIVELAFDATRFRVEYDIYSPEDNYKRVIYRSLNNAKFVNTNPVKFPGFARSSDVALILHTSGTTSTPKTVPLLHLNIVRSTLNIANTYKLTPLDRSYVVMPLFHVHGLIGVLLSTFRTQGSVVVPDGFHPKLFWDQFVKYNCNWFSCVPTISMIMLNMPKPNPFPHIRFIRSCSSALAPATFHKLEKEFNAPVLEAYAMTEASHQMTSNNLPPGKRKPGTVGQPQGVTVVILDDNDNVLPPGKVGEVSIRGENVTLGYANNPKANKENFTKRENYFRTGDQGYFDPEGFLVLTGRIKELINRGGEKISPIELDGIMLSHPKIDEAVAFGVPDDMYGQVVQAAIVLKKGEKMTYEELVNFLKKHLASFKIPTKVYFVDKLPKTATGKIQRRVIAETFAKSSRNKSKL
SEQ ID NO:48(来自动物双歧杆菌乳酸亚种DSM 10140的草酸盐:甲酸盐反向转运蛋白(oxlT)；UniProt Accession B9X293；Ba_OxlT)：
MTKILIDDIIPIIVIMALGYVCGKLSYFDNDQRQGLNKLVLNIALPAALFISIVKATREMLAQDAVLTILGFIGIIVMFMLSYYLCRLMFHHSIQEAAVCALIAGSPTIGFLGFAVLDPIYGDTVSTNLVIAIISIVVNAVTIPIGMYLINLGQSKDRERLSKAAVTNSKGQVSIANPKDDIAVDPNKDAKTDKTAEVMISKSSNMGKKKNQNLEALINALKQPVCWAPLLAIVLVLIGVRVPSGFAPTFDLIAKANSGVAVLAAGLALSTVKFSLGWETIWNTFYRLILTPAAFLGVGLLLGMGSNVNKLSMLVMAVALPPAFSGIIISSRYNIYVKEGASTTAVSTVAFAVTCLLWIWLVPLCCH
SEQ ID NO:49(来自草酸盐贪铜菌ATCC 11883的草酸盐:甲酸盐反向转运蛋白(oxlT)；Co_OxlT1)：
MSNATAGALSPNQGASESTRWIQLLVGVVCMIATANIQYAWTLFVPEIQDTYGWSRASIQIAFTVFVLVQTWLAPIEGYFIDKFGPRMMVAFGALFIGTAWVINSQATTLMGFYVGAAVGGLGVGSIYATCINNALKWFPDRRGLAVGLTAGGYGAGSAATILPIAAMIESQGFQHTFLFFGLLQGSLAFVAAWFLRSPKTGEVRGSKKLAQATRDYTLKEALCTRLFWLMLVMFVLVVTGGMMAVAQLGVIAKDLGVKEFQVDLHFFVMAALPLALMLDRIMNGISRPLFGWISDNIGREKTMVIAFTLEGLGIIALGYFGSNPYAFLILSGVVFLAWGEVYSLFSALAGDAFGTKHIGKIYGVLYTAKGIGALFVPIGNLLMEATGTWSTVLYTVAVMDLTAAFLAIMVLRPVLASHVATSRQRFAQESAAAGAQVAA
SEQ ID NO:50(来自草酸盐贪铜菌ATCC 11883的草酸盐:甲酸盐反向转运蛋白(oxlT)；Co_OxlT2)：
MELQQRESTSRFASPWVQLVFGVICMAMIANMQYGWTLFVNPIDDKYHWGRTAIQVAFTIFVVTETWLVPIEGYLVDKYGPRPVVVGGGLLCAIAWALNSVASSLPMLYFAAAIGGVGAGAVYGTCVGNALKWFPNRRGLAAGITAAGFGAGSAMTVVPIANMIKTSGYEATFLWFGLGQGLIVFILGLALYPPSARILSEVKTTLKAAATYNATPRQVLKSPIFWIMYAMFVMMAAGGLMATAQLGPIAKDFGLHDSPVSILGLTLPALTFALTIDRVLNGLTRPFFGWISDHIGRERTMFFAFAVEAVGILLLSKYGHNPVAFVVLTGIVFFAWGEIYSLFPATCGDTFGPKFAATNAGLLYTAKGTAALLVPFSSVITAATGDWHAVFMLASGMAALSAVLALFVLKPMREAHARKYVHANTAAPMGYRAVQEDLT
SEQ ID NO:51(来自大肠杆菌MG1655的草酸盐:甲酸盐反向转运蛋白(oxlT)；UniProt Accession P37662；Ec_YhjX)：
MTPSNYQRTRWLTLIGTIITQFALGSVYTWSLFNGALSAKLDAPVSQVAFSFGLLSLGLAISSSVAGKLQERFGVKRVTMASGILLGLGFFLTAHSDNLMMLWLSAGVLVGLADGAGYLLTLSNCVKWFPERKGLISAFAIGSYGLGSLGFKFIDTQLLETVGLEKTFVIWGAIALLMIVFGATLMKDAPKQEVKTSNGVVEKDYTLAESMRKPQYWMLAVMFLTACMSGLYVIGVAKDIAQSLAHLDVVSAANAVTVISIANLSGRLVLGILSDKIARIRVITIGQVISLVGMAALLFAPLNAVTFFAAIACVAFNFGGTITVFPSLVSEFFGLNNLAKNYGVIYLGFGIGSICGSIIASLFGGFYVTFYVIFALLILSLALSTTIRQPEQKMLREAHGSL
SEQ ID NO:52(来自产甲酸草酸杆菌DSM 4420的草酸盐:甲酸盐反向转运蛋白(oxlT)；UniProt Accession Q51330；Of_oxlT)：
MNNPQTGQSTGLLGNRWFYLVLAVLLMCMISGVQYSWTLYANPVKDNLGVSLAAVQTAFTLSQVIQAGSQPGGGYFVDKFGPRIPLMFGGAMVLAGWTFMGMVDSVPALYALYTLAGAGVGIVYGIAMNTANRWFPDKRGLASGFTAAGYGLGVLPFLPLISSVLKVEGVGAAFMYTGLIMGILIILIAFVIRFPGQQGAKKQIVVTDKDFNSGEMLRTPQFWVLWTAFFSVNFGGLLLVANSVPYGRSLGLAAGVLTIGVSIQNLFNGGCRPFWGFVSDKIGRYKTMSVVFGINAVVLALFPTIAALGDVAFIAMLAIAFFTWGGSYALFPSTNSDIFGTAYSARNYGFFWAAKATASIFGGGLGAAIATNFGWNTAFLITAITSFIAFALATFVIPRMGRPVKKMVKLSPEEKAVH
SEQ ID NO:53(来自动物双歧杆菌乳酸亚种DSM 10140的草酰辅酶A脱羧酶(OXC)；EC 4.1.1.8；UniProt Accession B9X297；Ba_oxc)：
MVDVSVTATSSDQNLTDSPHYLAETLIKNGVKHMYGVVGIPVTDFARIAQGMGIRFIGMRHEEDAVNAAAAEGFLTGRPAVALTVSAPGFLNGLAPLLEATTNGFPVIMIGGSSTRHVVDMHEGEYEGLDQMNYAKQFCKESFRIDKIEDIPLAVARAMHIACSGRPGGVYIDFPDDAVAQTLDKDVAESQLWVANQPAPAMPPAQSSVDEALKLLSEAKNPLMLVGKGAALAQAEDELREFVEKTDMPFQPMSMAKGVIPDDDPHCTASCRGLALRTADVVLLVGARLNWMLNFGEGKEWNPNVKFIQIDIDPNEIENARSIACPVVGDIKSAMQMINAGLEKTPVKASAQWLDMLKADAEKNDAKFAARVNSNTVPMGHYDALGAIKKVYDQHKDMILTNEGANTLDDCRNIIDIYQPRHRLDCGTWGVMGCAVGYSIGAAVATGKPVLYVGGDSGFGFDGMEVEVACRYNLPITFVVLNNGGIYRGDFENLGDDGDPSPLTLSYDAHYERMIEAFGGNGYYATTPAEVEQMVGEAVASGKPSLVHVQLADYAGKESGHISNLNPKPVVGPLATSEMTANPYLKGAHM
SEQ ID NO:54(来自草酸盐贪铜菌ATCC 11883的草酰辅酶A脱羧酶(OXC)；EC4.1.1.8；Co_oxc)：
MAEVGNELQRHAAEEHQAQTDGFHLVIDALKLNGIENIYGLPGIPVTDLARLAQANGMRVISFRHEQNAGNAAAIAGFLTQKPGVCLTVSAPGFLNGLTALAHATTNCFPMILISGSSEREIVDLQQGDYEEMDQLAIARPHAKAAFRVLHAEDIGVGIARAIRAAVSGRPGGVYLDLPAKLLGQSMEAEKGRKSLIKVVDPAPRQLPAPDSVDRAVALLKSAKRPLILVGKGAAYARAEADIRTLVEKTGIPYLPMSMAKGLLPDTHPQSASAARSYVLAEADVVLLVGARLNWLLSHGKGKTWGKPKQFIQIDIAPTEMDSNVAIAAPVVGDIGSCVSAILDKVGDDFARPGADWLNAVADRRDTNLAKMAETLARSRDASPMNFHGALGVLKDVVKANPNISFVNEGANTLDYARAVIDMYEPRKRLDVGTWGVMGVGMGYAVAAAVETGKPVLALCGDSAFGFSGMEVETICRYNLPVCIVIFNNNGVYKGIDVNPTGGRDPAVTTFVPGARYDKMMEAFGGVGANVTTPAELEAAVNEALRSGKPTLVNAVIDPAAGTESGRLTNLNPQSSAKK
SEQ ID NO:55(来自大肠杆菌MG1655的草酰辅酶A脱羧酶(OXC)；EC 4.1.1.8；UniProt Accession P0AFI0；Ec_oxc)：
MSDQLQMTDGMHIIVEALKQNNIDTIYGVVGIPVTDMARHAQAEGIRYIGFRHEQSAGYAAAASGFLTQKPGICLTVSAPGFLNGLTALANATVNGFPMIMISGSSDRAIVDLQQGDYEELDQMNAAKPYAKAAFRVNQPQDLGIALARAIRVSVSGRPGGVYLDLPANVLAATMEKDEALTTIVKVENPSPALLPCPKSVTSAISLLAKAERPLIILGKGAAYSQADEQLREFIESAQIPFLPMSMAKGILEDTHPLSAAAARSFALANADVVMLVGARLNWLLAHGKKGWAADTQFIQLDIEPQEIDSNRPIAVPVVGDIASSMQGMLAELKQNTFTTPLVWRDILNIHKQQNAQKMHEKLSTDTQPLNYFNALSAVRDVLRENQDIYLVNEGANTLDNARNIIDMYKPRRRLDCGTWGVMGIGMGYAIGASVTSGSPVVAIEGDSAFGFSGMEIETICRYNLPVTIVIFNNGGIYRGDGVDLSGAGAPSPTDLLHHARYDKLMDAFRGVGYNVTTTDELRHALTTGIQSRKPTIINVVIDPAAGTESGHITKLNPKQVAGN
SEQ ID NO:56(来自产甲酸草酸杆菌DSM 4420的草酰辅酶A脱羧酶(OXC)；EC4.1.1.8；UniProt Accession P40149；Of_oxc)：
MSNDDNVELTDGFHVLIDALKMNDIDTMYGVVGIPITNLARMWQDDGQRFYSFRHEQHAGYAASIAGYIEGKPGVCLTVSAPGFLNGVTSLAHATTNCFPMILLSGSSEREIVDLQQGDYEEMDQMNVARPHCKASFRINSIKDIPIGIARAVRTAVSGRPGGVYVDLPAKLFGQTISVEEANKLLFKPIDPAPAQIPAEDAIARAADLIKNAKRPVIMLGKGAAYAQCDDEIRALVEETGIPFLPMGMAKGLLPDNHPQSAAATRAFALAQCDVCVLIGARLNWLMQHGKGKTWGDELKKYVQIDIQANEMDSNQPIAAPVVGDIKSAVSLLRKALKGAPKADAEWTGALKAKVDGNKAKLAGKMTAETPSGMMNYSNSLGVVRDFMLANPDISLVNEGANALDNTRMIVDMLKPRKRLDSGTWGVMGIGMGYCVAAAAVTGKPVIAVEGDSAFGFSGMELETICRYNLPVTVIIMNNGGIYKGNEADPQPGVISCTRLTRGRYDMMMEAFGGKGYVANTPAELKAALEEAVASGKPCLINAMIDPDAGVESGRIKSLNVVSKVGKK
SEQ ID NO:57(来自酿酒酵母S288C的草酰辅酶A脱羧酶(OXC)；EC 4.1.1.8；UniProt Accession P39994；Sc_YEL020C)：
MTTTATQHFAQLLQKYGIDTVFGIVGIPIVQLADTMVANGIKFIPCRNEQAASYAASAYGYISDKPGVLLIVGGPGLIHALAGIYNSMSNRWPLLVIAGSSSQSDIHKGGFQELDQVSLLSPFLKFTGKLTPDNIDMITQKALNYCIQGTAGVSYIDVPADFIEYEKPLEGNDRTGNELPMILTPNICGPDPSKIKKVVQLILQHKNKNILIVIGKGAVKNSHEIRRLVNTFNLPFLPTPMAKGIVPDSSPLNVSSARSQALKIADIVLVLGARLNWILHFGTSPKWNSESIFIQFDSNPETLGDNNVSPGADLSIWGDIGLSVTALVEELTRQDSCWKYSGVKQEIREKIQLNQTRLLRKEKTRGAQLNYNQVYGTLRPLIDDYRTILVTEGANTMDIARISFPTDAPRRRLDAGTNATMGIGLGYALACKASHPELDVVLIQGDSAFGFSAMEIETAVRCQLALVIVVMNNSGIYHGEKDIEGDLPPTALSKNCRYDLVGKGLGANDFFVNTISELSRCFQQAVQLSRTKRETSVINVIIEPGEQKQIAFAWQNKPRL
SEQ ID NO:58(来自扭脱甲基杆菌AM1/DSM 1338的草酰辅酶A还原酶/乙醛酸盐:NADP+氧化还原酶(OXR)；EC 1.2.1.17；UniProt Accession C5APN9；Me_PanE)：
MSIAIVGAGAIGGYLGVRLAEAGEDVTFIARSNAAAIQADGMRLIEEDGTEIHSKSVKATRSMQEAGVHEVVLLTVKAHQVGPIAADLHHLIGPDTVVVTMQNGIPWWYFLGGYSGDHAGTRLESADPGGLIADHLDPKHVIGSVVYPATVLTDPGTVKVIEGNRFGLGELDGSKSERVLALSQRLARAGFRAPVTSDIRAEIWLKLWGNLSFNPISALTHATLEDICRFPDTRAIAAEMMREAEVIANKLGVTFRLGIDKRIAGAEKVGPHKTSMLQDVEAGRPIELEALVGSVIELGRLTGTPTPHIDTVFALMRLLAQSLERAQGRLAIQGA
SEQ ID NO:59(来自草酸盐贪铜菌ATCC 11883的假定琥珀酰辅酶A:草酸辅酶A转移酶(SCOCT)；EC 2.8.3.2；Co_ACOCT)：
MTQSNLPDLPDLPLTGLRVIDFSRVLAGPYCTALLGDLGAEVIKVEPPGGDDYRAVGPFAGGKSGLFCAMNRNKQSIVIDLKTEDGLAVARALCRGADVVVENFRPGVADKLGIGYAALRELNPSLVYASVSGFGQTGPESHRPAYDIILQAMCGLMDATGAPDGAPTMLGEAVSDAVSGLFASWGVLAALLAREKTGRGTHVDVSMFDATLSLSATLVARYAATGLAPRRVGNRHPSSAPFGAYRAADGFYVVAVLNNKLFQALADAIGRPAMAHDPRFADDETRCRFESDLRAMLENWSATRTVAEVNAALGAAGIPVAPIRNVKEALESEHAAHRRLLTEVPDTDGGTVRLPSQPVKFSAYGTNRVTPAPALGQHTGAILAAPDFSNFNKEKQHA
SEQ ID NO:60(来自草酸盐贪铜菌ATCC 11883的假定琥珀酰辅酶A:草酸辅酶A转移酶(SCOCT)；EC 2.8.3.2；Co_CaiB)：
MGALSHLRVLDLTRVLAGPWCAQNLADFGADVIKIERPGAGDDTRTWGPPWLKDEDGRDTAEAAYYLAANRNKRSVTCDISTPEGQQIVRDLAAQSDVVLENYKVGQLKKYGLDYESLRQVKPDLIYCSVTGFGQTGPYAARPGYDFIIQGMGGFMSLTGERDDLPGGGPQKAGVAISDLMTGQYATIAVLAALAHRERTGEGQYIDMALLDVQVAMLANMNTNYLASGEAPRRWGNAHPNIVPYQTFQAADGWIIVAVGNDGQFRKFVTDGGMPELADDPRFAKNPQRVANRDVLVPILAEMVRPRTRAQWIRDLEAAGVPCGPINTLDDVFEDDQVKARGLRVDLPHPSAGEVKLVASPIKMSATPPQALRHPPLLGEHTDTVLAETLGYSAGQIAALRAKGVL
SEQ ID NO:61(来自草酸盐贪铜菌ATCC 11883的假定琥珀酰辅酶A:草酸辅酶A转移酶(SCOCT)；EC 2.8.3.2；Co_SCACT)：
MDKERIRLARLHDKVVSADEAAALIRDGMTVGMSGFTRAGDCKEVPFALARRAATEPLRITLMTGASLGNDIDRVLAEADVIARRLPFQSDGTLRRKINAGEVMFIDQHLSETVEQLRSGQIAPVDVAVVEAVAITEQGGIIPSTSVGNSASFAMLARKVIVEINMNMPLELEGLHDIYFPVQRPYRQPIPLIAPEQRIGLPYIPIDPEKIAAIVITAKDDSPSNALPPDDETRSIAGHLNDFLLREVRAGKLSPSLQPLQAGIGTIANAVLHGLVESPFRDLKMYSEVLQDSTIELLDAGRLSFASASSVTLTREVYQRFLSNLDRYRSRLLLRPQEISNHPEILRRLGLITINTALECDIYGNVNSTHVGGTHMMNGIGGSGDFARNAHVSVFVTKSVAKGGEVSSIVPMVAHVDHTEHDVDIIVTEHGLADLRGLAPRERARTVIANCADPQYRELLGDYFRRASAHGGQTPHLLEEALSWHVGLRDRGTMRSAQPAQALAA
SEQ ID NO:62(来自草酸盐贪铜菌ATCC 11883的假定琥珀酰辅酶A:草酸辅酶A转移酶(SCOCT)；EC 2.8.3.2；Co_SCMCT)：
MVNEANKEKMPLQGVRVLELGSLIAGPYAGGLLAQFGAEVLKIEAPGEGDPLRKWRKLYEGTSLWWYSQSRNKKSLTLDLRSAQGQDIVRKLVAGADIVIENFRPGTLEKWGLGWEDLRKVNPALVMVRISGYGQTGPYKDRPGFAAIAESMGGLRYVSGYPDRPPVRSGVSIGDTLASLYGVIGALLAMHHLRNNDGEGQFVDVALYESVFAVMESLVPEFDKFGHVRERSGASLPGISPSNTYPCQDGQYVIIAGNGDAIFRRFMTAIGRDDLGQDPRLAHNDGRVQHNAMLDDAIAAWTAAHDLDTVLATLEAAAVPCGRIYTAQDICSDPHYSARGMIEPHQLPDGSPLALSGIVPRLSETPGRTNWIGPALGEHTEEILAGIGIAGAEFRALREAGVV
SEQ ID NO:63(pZR748-Sc_YBR222C,Sc_YEL020C,Of_oxlT表达载体)：
GGAGAGCTGTATGTGGGTAGGATAAAACGAAAGGCTCAGTCGAAAGACTGGGCCTTTCGTTTTACAATTGGGCTACCTTTTTTTTGTTTTGTTTGCAATGGTTAATCTATTGTTAAAATTTAAAGTTTCACTTGAACTTTCAAATAATGTTCTTATATTTGCAGTGTCGAAAGAAACAAAGTAGCCTGGATCACACAACATTTAAAAAATAACATTATGAAAGCACTCGAAAAGAGATTCAAAGGTAATATTAACAATAATTTATTTTCAATGACAAGTGCCGCTACTGTTACTGCTTCGTTCAACGATACTTTTAGCGTATCCGATAATGTCGCCGTTATTGTTCCTGAAACGGACACTCAGGTGACCTACAGGGATCTATCCCACATGGTGGGTCACTTCCAGACCATGTTCACAAATCCTAATTCTCCATTGTACGGAGCTGTTTTCAGACAAGATACAGTGGCGATATCCATGCGTAATGGGCTGGAATTTATCGTCGCTTTCCTCGGTGCTACTATGGACGCTAAAATTGGCGCGCCCTTGAATCCCAATTATAAGGAAAAGGAGTTCAATTTTTATTTGAATGACCTGAAATCTAAGGCGATTTGCGTCCCAAAGGGTACCACAAAGTTACAGAGTTCTGAAATTCTAAAATCTGCCTCCACGTTTGGATGTTTTATCGTAGAGCTGGCCTTCGATGCGACCAGGTTTAGGGTAGAGTATGATATATACTCTCCAGAGGACAACTACAAAAGGGTTATTTACCGGTCTTTGAACAACGCCAAATTTGTCAACACAAATCCCGTTAAATTCCCTGGGTTTGCCCGTTCCAGTGACGTTGCCCTGATTTTGCATACCAGTGGTACCACCTCCACTCCAAAAACGGTGCCTTTGTTACATTTGAACATTGTGAGAAGCACGTTGAACATTGCTAACACTTACAAGCTAACGCCCTTGGACAGATCTTATGTCGTGATGCCTCTTTTCCACGTCCATGGGTTAATTGGTGTTTTACTTTCCACTTTTAGAACTCAGGGTTCTGTTGTGGTTCCCGATGGATTCCATCCAAAGTTATTCTGGGACCAATTTGTTAAGTACAACTGTAATTGGTTCAGTTGCGTTCCCACAATAAGCATGATTATGCTGAACATGCCCAAACCAAACCCTTTCCCACACATTAGATTCATCAGATCGTGTTCTTCTGCTTTGGCTCCAGCAACGTTCCATAAGCTGGAGAAGGAATTCAATGCACCTGTCTTGGAGGCCTATGCGATGACCGAAGCATCACATCAAATGACCTCAAACAATCTGCCTCCAGGAAAGAGAAAGCCTGGTACTGTGGGCCAGCCACAAGGAGTCACCGTCGTCATTCTAGATGACAATGACAATGTCTTGCCCCCGGGCAAAGTCGGCGAAGTTTCCATCAGAGGCGAAAACGTCACTTTGGGGTATGCTAATAATCCAAAAGCTAACAAGGAGAACTTCACCAAGAGAGAGAACTATTTCAGAACCGGTGACCAAGGTTATTTCGACCCTGAGGGGTTTTTGGTCCTTACAGGCAGAATCAAAGAGCTTATCAACAGGGGTGGTGAAAAGATTTCACCCATTGAGCTCGACGGCATTATGCTATCGCATCCAAAGATCGATGAAGCCGTTGCATTTGGTGTTCCCGACGATATGTACGGCCAAGTAGTTCAAGCCGCCATTGTTTTGAAGAAGGGAGAAAAAATGACCTACGAAGAACTGGTGAACTTCTTAAAGAAGCACCTAGCCTCTTTCAAAATTCCAACCAAGGTGTACTTTGTTGATAAGCTACCAAAAACCGCTACAGGTAAAATCCAGAGAAGAGTTATCGCAGAAACTTTTGCTAAGAGCAGCAGAAATAAGAGTAAGTTGTAGGGTTCCTAGCTGATTAGAAGGCCATCCTGACGGATGGCCTTTTTTTTGACTGGTAAGCCAATCTAGGGCCTCGTCTGTAGGATAAAACGAAAGGCTCAGTCGAAAGACTGGGCCTTTCGTTTTACAATTGGGCTACCTTTTTTTTGTTTTGTTTGCAATGGTTAATCTATTGTTAAAATTTAAAGTTTCACTTGAACTTTCAAATAATGTTCTTATATTTGCAGTGTCGAAAGAAACAAAGTAGCCTGGATCACACAACATTTAAAAAATAACATTATGAAAGCACTCGAAAAGAGATTCAAAGGTAATATTAACAATAATTTATTTTCAATGACCACGACCGCTACCCAACACTTCGCTCAACTTTTACAAAAATATGGCATCGACACAGTCTTTGGAATTGTGGGCATCCCTATTGTCCAACTAGCGGATACGATGGTTGCCAACGGTATCAAATTCATTCCATGCAGGAACGAACAGGCTGCCTCATATGCAGCCTCTGCTTATGGTTATATTAGTGATAAGCCCGGCGTGTTACTTATTGTCGGGGGCCCCGGTTTAATCCACGCACTGGCTGGTATATACAACTCAATGAGTAATAGGTGGCCTCTTCTAGTAATTGCAGGGAGTTCTTCTCAAAGTGATATACATAAAGGCGGGTTTCAAGAGTTGGATCAAGTTAGTCTTTTGTCCCCATTTTTGAAGTTTACCGGAAAGTTAACCCCTGACAATATTGACATGATTACTCAGAAGGCTTTGAATTACTGTATACAAGGCACTGCAGGGGTTTCTTACATAGACGTCCCTGCAGATTTTATTGAGTATGAAAAACCCTTAGAAGGAAACGACCGTACAGGAAATGAATTACCGATGATATTAACTCCAAACATATGTGGCCCTGATCCGTCGAAGATCAAAAAAGTCGTACAGCTTATTTTGCAACATAAAAATAAAAATATTCTGATTGTTATTGGAAAGGGTGCGGTAAAAAACTCACACGAAATCCGCAGACTAGTAAATACGTTCAATCTACCATTTTTACCCACCCCAATGGCTAAGGGAATTGTCCCAGACTCTTCTCCACTGAACGTTTCATCTGCAAGATCTCAAGCATTGAAAATAGCGGATATTGTTCTTGTTCTCGGGGCAAGATTAAACTGGATATTGCATTTTGGTACTTCACCCAAATGGAACTCAGAATCCATATTCATCCAGTTCGATTCCAATCCCGAAACCTTGGGGGATAACAATGTCTCACCTGGTGCTGACCTTTCCATATGGGGAGATATAGGCTTGAGCGTAACCGCTTTAGTTGAGGAATTGACTCGCCAAGATTCCTGCTGGAAGTATAGTGGTGTTAAGCAAGAAATACGAGAGAAAATTCAACTGAATCAAACTCGTCTATTGAGGAAAGAGAAGACTAGAGGGGCACAATTGAATTACAACCAGGTTTATGGAACATTGAGGCCTCTTATCGATGATTACAGGACAATACTTGTGACGGAGGGAGCAAATACTATGGATATTGCGCGTATTTCATTCCCTACAGACGCTCCAAGGCGCCGTTTAGACGCAGGGACCAACGCGACTATGGGGATTGGGCTCGGATATGCTCTTGCATGCAAGGCATCTCATCCGGAACTCGATGTGGTGCTGATTCAGGGTGATTCCGCATTTGGATTCTCTGCCATGGAAATTGAAACGGCAGTAAGGTGTCAGCTGGCATTGGTCATCGTTGTGATGAACAATAGCGGTATTTACCATGGAGAAAAGGATATAGAGGGTGATTTACCTCCAACGGCACTAAGCAAGAACTGCCGTTACGATCTCGTAGGCAAAGGACTGGGTGCCAATGATTTTTTTGTCAACACAATAAGCGAACTGAGCAGATGCTTCCAACAAGCCGTGCAGCTTTCTCGGACTAAAAGAGAAACAAGTGTCATCAACGTTATCATTGAGCCTGGCGAACAAAAGCAAATTGCCTTTGCCTGGCAGAATAAACCGCGTTTATAGGGTTCCTAGCTGATTAGAAGGCCATCCTGACGGATGGCCTTTTTTTTGACTGCGTTCCTGGCGTGGTGCTCGCATAGTAGGATAAAACGAAAGGCTCAGTCGAAAGACTGGGCCTTTCGTTTTACAATTGGGCTACCTTTTTTTTGTTTTGTTTGCAATGGTTAATCTATTGTTAAAATTTAAAGTTTCACTTGAACTTTCAAATAATGTTCTTATATTTGCAGTGTCGAAAGAAACAAAGTAGCCTGGATCACACAACATTTAAAAAATAACATTATGAAAGCACTCGAAAAGAGATTCAAAGGTAATATTAACAATAATTTATTTTAATGAATAATCCACAAACAGGACAATCAACAGGCCTCTTGGGCAATCGTTGGTTCTACTTGGTATTAGCAGTTTTGCTGATGTGTATGATCTCGGGTGTCCAATATTCCTGGACACTGTACGCTAACCCGGTTAAAGACAACCTTGGCGTTTCTTTGGCTGCGGTTCAGACGGCTTTCACACTCTCTCAGGTCATTCAAGCTGGTTCTCAGCCTGGTGGTGGTTACTTCGTTGATAAATTCGGTCCAAGAATTCCATTGATGTTCGGTGGTGCGATGGTTCTCGCTGGCTGGACCTTCATGGGTATGGTTGACAGTGTTCCTGCTCTGTATGCTCTTTATACTCTGGCCGGTGCAGGTGTTGGTATCGTTTACGGTATCGCGATGAACACGGCTAACAGATGGTTCCCGGACAAACGCGGTCTGGCTTCCGGTTTCACCGCTGCCGGTTACGGTCTGGGTGTTCTGCCGTTCCTGCCACTGATCAGCTCCGTTCTGAAAGTTGAAGGTGTTGGCGCAGCATTCATGTACACCGGTTTGATCATGGGTATCCTGATTATCCTGATCGCTTTCGTTATCCGTTTCCCTGGCCAGCAAGGCGCCAAAAAACAAATCGTTGTTACCGACAAGGATTTCAATTCTGGCGAAATGCTGAGAACACCACAATTCTGGGTTCTGTGGACCGCATTCTTTTCCGTTAACTTTGGTGGTTTGCTGCTGGTTGCCAACAGCGTCCCTTACGGTCGCAGCCTCGGTCTTGCCGCAGGTGTGCTGACGATCGGTGTTTCGATCCAGAACCTGTTCAATGGTGGTTGCCGTCCTTTCTGGGGTTTCGTTTCCGATAAAATCGGCCGTTACAAAACCATGTCCGTCGTTTTCGGTATCAATGCTGTTGTTCTCGCACTTTTCCCGACGATTGCTGCCTTGGGCGATGTAGCCTTTATCGCCATGTTGGCAATCGCATTCTTCACATGGGGTGGTAGCTACGCTCTGTTCCCATCGACCAACAGCGATATTTTCGGTACGGCATACTCTGCCAGAAACTATGGTTTCTTCTGGGCTGCAAAAGCAACTGCCTCGATCTTCGGTGGTGGTCTGGGTGCTGCAATTGCAACCAACTTCGGATGGAATACCGCTTTCCTGATTACTGCGATTACTTCTTTCATCGCATTTGCTCTGGCTACCTTCGTTATTCCAAGAATGGGCCGTCCAGTCAAGAAAATGGTCAAATTGTCTCCAGAAGAAAAAGCTGTACATTAAGGTTCCTAGCTGATTAGAAGGCCATCCTGACGGATGGCCTTTTTTTTGACTGCTATGACTAAACGGCGCGCCTGATAGGTGGGCTGCCCTTCCTGGTTGGCTTGGTTTCATCAGCCATCCGCTTGCCCTCATCTGTTACGCCGGCGGTAGCCGGCCAGCCTCGCAGAGCAGGATTCCCGTTGAGCACCGCCAGGTGCGAATAAGGGACAGTGAAGAAGGAACACCCGCTCGCGGGTGGGCCTACTTCACCTATCCTGCCCGGCTGACGCCGTTGGATACACCAAGGAAAGTCTACACGAACCCTTTGGCAAAATCCTGTATATCGTGCGAAAAAGGATGGATATACCGAAAAAATCGCTATAATGACCCCGAAGCAGGGTTATGCAGCGGAAAAGCGGGATTAAAAGTCGGGGATTGGTGAACAAAAAGGTGTTTCTCTCTTTAAGAGAAATATCGTTTTGCTAAACAGTTGATATTGAGGTATCATTTTATCGTAAAAGACATTTTTGCTCAACAATTGCTTGACGGAAATCAACAAATTTTAGCATTTTGTAAAAAAGTCGCTATATAATTTGGTGAATTGGAGTTATTTTCATATTTTTGCATCCCGAAGAGTTTCTCTTAAAGAGAGAAACATCTTTTGCATACCTTTTCCGACCGAATTTTTATGTCGTAAAGAGGGGCTTTGCAGGGGGTGGACTCAGAAAGATGAGAATAGATGACTATTGTAGTTGAAACACATAGAAAGTTGCTGATATACAGACCGATACGCATATCGGGATGAACCATGAGTACGTTCTTTTCTCAAAAAACATAAATATTCGAAAAGAGATGCAATAAATTAAGGAGAGGTTATAATGAACAAAGTAAATATAAAAGATAGTCAAAATTTTATTACTTCAAAATATCACATAGAAAAAATAATGAATTGCATAAGTTTAGATGAAAAAGATAACATCTTTGAAATAGGTGCAGGGAAAGGTCATTTTACTGCTGGATTGGTAAAGAGATGTAATTTTGTAACGGCGATAGAAATTGATTCTAAATTATGTGAGGTAACTCGTAATAAGCTCTTAAATTATCCTAACTATCAAATAGTAAATGATGATATACTGAAATTTACATTTCCTAGCCACAATCCATATAAAATATTTGGCAGCATACCTTACAACATAAGCACAAATATAATTCGAAAAATTGTTTTTGAAAGTTCAGCCACAATAAGTTATTTAATAGTGGAATATGGTTTTGCTAAAATGTTATTAGATACAAACAGATCACTAGCATTGCTGTTAATGGCAGAGGTAGATATTTCTATATTAGCAAAAATTCCTAGGTATTATTTCCATCCAAAACCTAAAGTGGATAGCACATTAATTGTATTAAAAAGAAAGCCAGCAAAAATGGCATTTAAAGAGAGAAAAAAATATGAAACTTTTGTAATGAAATGGGTTAACAAAGAGTACGAAAAACTGTTTACAAAAAATCAATTTAATAAAGCTTTAAAACATGCGAGAATATATGATATAAACAATATTAGTTTCGAACAATTTGTATCGCTATTTAATAGTTATAAAATATTTAACGGCTAAAAACAATAGGCCACATGCAACTGTAAATGTTTACGCGGGTACCGACACCGCGGTGGAGGGGAATTACGAGTCATTGGTAACTATCTATGAAACTGTTTGATACTTTTATAGTTGATTAAACTTGTTCATGGCATTTGCCTTAATATCATCCGCTATGTCAATGTAGGGTTTCATAGCTTTGTAGTCGCTGTGTCCCGTCCATTTCATGACCACCTGTGCCGGGATTCCGAGAGCCAGCGCATTGCAGATGAATGTCCTTTTTCCTGCATGGGTACTGAGCAAAGCGTATTTGGGTGTGACTTCATCAATACGTTCATTTCCCTTGTAGTAGGTTTCCCGTACAGGCTCGTTGATTTCTGCCAGTTCGCCCAGCTCTTTCAGGTAATCGTTCATCTTCTGGTTGCTGATGACGGGCAGAGCCATGTAATTCTCGAAATGGATGTCCTTGTATTTGTCCAGTATGGCTTTGCTGTATTTGTTCAGTTCAATCGTCAGGCTGTCGGCAGTCTTGACTGTGGTTATTTCGATGTGGTCGGACTTCACATCGCTTCTTTTCAGATTGCGAACATCCGAATACCGCAAACTCGTAAAGCAGCAGAACAGGAAAACATCACGCACACGTTCCAGGTATTGCTTATCCTTGGGTATCTGGTAGTCTTTCAGCTTGTTCAGTTCATCCCAAGTCAGGAAGATTACTTTTTTCGAGGTGGTTTTCAGTTTCGGTTTGAACGTATCGTATGCAATGTTCTGATGATGTCCTTTCTTGAAGCTCCAGCGCAGGAACCATTTGAGGAATCCCATTTGCTTGCCGATGGTGCTGTTTCTCATATCCTTGGTGTCACGCAGGAAGTTGACGTATTCGTTCAATCCAAACTCGTTGAAATAGTTGAACGTTGCATCCTCCTTGAACTCTTTGAGGTGGTTCCTCACTGCTGCAAATTTTTCATAGGTGGATGCCGTCCAGTTATTCTGGTTACCGCACTCTTTTACAAACTCATCGAACACCTCCCAAAAGCTGACAGGGGCTTCTTCCGGCTGTTCTTCACTGGTATCTTTCATTCTCATGTTGAAAGCTTCCTTCAACTGTTGGGTCGTTGGCATGACCTCCTGCACCTCAAATTCCTTGAAAATATTCTGGATTTCGGCATAGTATTTCAGCAAGTCCGTATTGATTTCGGCTGCACTTTGCTTTAGCTTGTTGGTACATCCGTTCTTTACCCGCTGCTTATCTGCATCCCATTTGGCTACGTCAATCCGGTAGCCCGTTGTAAACTCGATACGTTGGCTGGCAAAGATGACACGCATACGGATGGGTACGTTCTCTACGATTGGCACACCGTTCTTTTTCCGGCTCTCCAATGCAAAAATGATGTTGCGCTTGATATTCATAATTGGGTGCGTTTGAAATTCTACACCCAAATATACACCCAATTATTGAGATAGCAAAAGACATTTAGAAACATTTACTTTTACTCTATATTGTAATTTACACTTGATTATCAGTCGTTTGCAGTCTTATGATATTCTGTGAAAGTATAAGTTCGAGAGCCTGTCTCTCCGCAAAAAACGCTGAAAATCAGCAGATTGCAAAACAAACACCCTGTTTTACACCCAAGAATGTAAAGTCGGGTGTTTTTGTTTTATTTAAGATAATACAACCACTACATAATAAAAGAGTAGCGATATTAAAAGAATCCGATGAGAAAAGACTAATATTTATCTATCCATTCAGTTTGATTTCTCAGGACTTTACATCGTCCTGAAAGTATTTGTTGCCAGTGTTACAACCAATTAACCAATTCTGATTAGAAAAACTCATCGAGCATCAAATGAAACTGCAATTTATTCATATCAGGATTATCAATACCATATTTTTGAAAAAGCCGTTTCTGTAATGAAGGAGAAAACTCACCGAGGCAGTTCCATAGGATGGCAAGATCCTGGTATCGGTCTGCGATTCCGACTCGTCCAACATCAATACAACCTATTAATTTCCCCTCGTCAAAAATAAGGTTATCAAGTGAGAAATCACCATGAGTGACGACTGAATCCGGTGAGAATGGCAAAAGCTTATGCATTTCTTTCCAGACTTGTTCAACAGGCCAGCCATTACGCTCGTCATCAAAATCACTCGCATCAACCAAACCGTTATTCATTCGTGATTGCGCCTGAGCGAGGCGAAATACGCGATCGCTGTTAAAAGGACAATTACAAACAGGAATCGAATGCAACCGGCGCAGGAACACTGCCAGCGCATCAACAATATTTTCACCTGAATCAGGATATTCTTCTAATACCTGGAATGCTGTTTTCCCGGGGATCGCAGTGGTGAGTAACCATGCATCATCAGGAGTACGGATAAAATGCTTGATGGTCGGAAGAGGCATAAATTCCGTCAGCCAGTTTAGTCTGACCATCTCATCTGTAACATCATTGGCAACGCTACCTTTGCCATGTTTCAGAAACAACTCTGGCGCATCGGGCTTCCCATACAATCGATAGATTGTCGCACCTGATTGCCCGACATTATCGCGAGCCCATTTATACCCATATAAATCAGCATCCATGTTGGAATTTAATCGCGGCCTGGAGCAAGACGTTTCCCGTTGAATATGGCTCATAACACCCCTTGTATTACTGTTTATGTAAGCAGACAGTTTTATTGTTCATGATGATATATTTTTATCTTGTGCAATGTAACATCAGAGATTTTGAGACACAACGTGGCTTTGTTGAATAAATCGAACTTTTGCTGAGTTGAAGGATCAGCCGCGCAGTTCAACCTGTTGATAGTACGTACTAAGCTCTCATGTTTCACGTACTAAGCTCTCATGTTTAACGTACTAAGCTCTCATGTTTAACGAACTAAACCCTCATGGCTAACGTACTAAGCTCTCATGGCTAACGTACTAAGCTCTCATGTTTCACGTACTAAGCTCTCATGTTTGAACAATAAAATTAATATAAATCAGCAACTTAAATAGCCTCTAAGGTTTTAAGTTTTATAAGAAAAAAAAGAATATATAAGGCTTTTAAAGCTTTTAAGGTTTAACGGTTGTGGACAACAAGCCAGGGATGTAACGCACTGAGAAGCCCTTAGAGCCTCTCAAAGCAATTTTGAGTGACACAGGAACACTTAACGGCTGACATGGGGCGGCCGCTCAAGTAG
SEQ ID NO:64(pZR753-Ec_frc,Of_oxc,Sc_YBR222C,Of_oxlT表达载体)：
GGAGAGCTGTATGTGGGTAGGATAAAACGAAAGGCTCAGTCGAAAGACTGGGCCTTTCGTTTTACAATTGGGCTACCTTTTTTTTGTTTTGTTTGCAATGGTTAATCTATTGTTAAAATTTAAAGTTTCACTTGAACTTTCAAATAATGTTCTTATATTTGCAGTGTCGAAAGAAACAAAGTAGCCTGGATCACACAACATTTAAAAAATAACATTATGAAAGCACTCGAAAAGAGATTCAAAGGTAATATTAACAATAATTTATTTTCAATGTCAACTCCACTTCAAGGAATTAAAGTTCTCGATTTCACCGGTGTGCAATCTGGCCCATCTTGTACTCAAATGCTGGCCTGGTTTGGCGCTGACGTTATTAAAATTGAACGTCCCGGCGTTGGTGACGTAACGCGTCACCAGCTGCGAGATATTCCTGATATCGATGCGCTTTACTTCACCATGCTTAACAGTAACAAACGTTCTATTGAGTTAAATACCAAAACAGCGGAAGGCAAAGAGGTAATGGAAAAGCTGATCCGCGAAGCTGATATCTTAGTCGAGAACTTTCATCCAGGGGCCATTGATCACATGGGCTTCACCTGGGAGCATATTCAAGAAATCAATCCACGTCTGATTTTTGGTTCGATCAAAGGGTTTGATGAGTGTTCGCCTTATGTGAATGTAAAAGCCTATGAAAACGTTGCTCAGGCAGCGGGTGGCGCGGCATCCACTACGGGTTTTTGGGATGGTCCGCCGCTGGTAAGCGCTGCAGCGTTGGGTGACAGCAACACCGGAATGCATTTGCTGATCGGTTTACTTGCTGCTTTGCTGCATCGCGAAAAAACGGGGCGTGGGCAACGAGTCACCATGTCAATGCAGGATGCCGTATTGAACCTTTGCCGCGTGAAATTACGTGACCAGCAGCGTCTAGATAAATTGGGTTATCTGGAAGAATACCCGCAGTATCCGAATGGTACATTTGGTGATGCAGTTCCCCGCGGTGGTAATGCAGGTGGTGGCGGTCAGCCTGGCTGGATCCTGAAATGTAAAGGCTGGGAAACCGATCCTAACGCCTATATTTATTTCACTATTCAGGAGCAAAACTGGGAAAACACCTGTAAAGCCATCGGCAAACCAGAATGGATTACCGATCCGGCATACAGTACAGCCCATGCACGACAGCCACATATTTTCGATATTTTTGCTGAAATCGAAAAATACACTGTCACTATTGATAAACATGAAGCGGTGGCCTATTTGACTCAGTTTGATATTCCTTGTGCACCGGTTTTAAGTATGAAAGAAATTTCACTTGATCCCTCTTTGCGCCAAAGTGGCAGTGTTGTTGAAGTGGAACAACCGTTGCGTGGAAAATATCTGACCGTTGGTTGTCCAATGAAATTCTCTGCCTTTACGCCGGATATTAAAGCTGCGCCGCTATTAGGTGAACATACCGCTGCTGTATTGCAGGAGCTGGGTTATAGCGACGATGAAATTGCTGCAATGAAGCAAAACCACGCCATCTGAGGTTCCTAGCTGATTAGAAGGCCATCCTGACGGATGGCCTTTTTTTTGACTGGTAAGCCAATCTAGGGCCTCGTCTGTAGGATAAAACGAAAGGCTCAGTCGAAAGACTGGGCCTTTCGTTTTACAATTGGGCTACCTTTTTTTTGTTTTGTTTGCAATGGTTAATCTATTGTTAAAATTTAAAGTTTCACTTGAACTTTCAAATAATGTTCTTATATTTGCAGTGTCGAAAGAAACAAAGTAGCCTGAGCTGTCACCGGATGTGCTTTCCGGTCTGATGAGTCCGTGAGGACGAAACAGCCTCTACAAATAATTTTGTTTAATCCATCAATTTAAAATTTAAAATAATGGTTTTTACTCTGGAAGATTTTGTTGGCGATTGGCGTCAGACCGCGGGTTATAATTTGGATCAAGTCCTGGAACAGGGTGGCGTAAGCTCTCTGTTCCAGAACCTGGGTGTGAGCGTGACGCCGATTCAGCGCATCGTTCTGTCCGGCGAGAACGGTCTGAAAATTGATATTCATGTGATCATCCCGTACGAAGGCCTGAGCGGTGACCAAATGGGTCAAATCGAGAAAATCTTTAAAGTCGTCTACCCAGTTGACGATCACCACTTCAAGGTTATCTTGCATTACGGTACGCTGGTGATTGATGGTGTGACCCCGAATATGATTGACTATTTCGGCCGTCCGTATGAAGGCATTGCCGTTTTTGACGGTAAAAAGATCACCGTCACCGGTACCCTGTGGAATGGCAATAAGATTATTGACGAGCGTCTGATTAACCCGGACGGCAGCCTGCTGTTCCGCGTGACCATCAACGGTGTCACGGGTTGGCGTCTGTGCGAGCGCATCCTGGCAGGTTCAATGAGTAACGACGACAATGTAGAGTTGACTGATGGCTTTCATGTTTTGATCGATGCCCTGAAAATGAATGACATCGATACCATGTATGGTGTTGTCGGCATTCCTATCACGAACCTGGCTCGTATGTGGCAAGATGACGGTCAGCGTTTTTACAGCTTCCGTCACGAACAACACGCAGGTTATGCAGCTTCTATCGCCGGTTACATCGAAGGAAAACCTGGCGTTTGCTTGACCGTTTCCGCCCCTGGCTTCCTGAACGGCGTGACTTCCCTGGCTCATGCAACCACCAACTGCTTCCCAATGATCCTGTTGAGCGGTTCCAGTGAACGTGAAATCGTCGATTTGCAACAGGGCGATTACGAAGAAATGGATCAGATGAATGTTGCACGTCCACACTGCAAAGCTTCTTTCCGTATCAACAGCATCAAAGACATTCCAATCGGTATCGCTCGTGCAGTTCGCACCGCTGTATCCGGACGTCCAGGTGGTGTTTACGTTGACTTGCCAGCAAAACTGTTCGGTCAGACCATTTCTGTAGAAGAAGCTAACAAACTGCTCTTCAAACCAATCGATCCAGCTCCGGCACAGATTCCTGCTGAAGATGCTATCGCTCGCGCTGCTGACCTGATCAAGAACGCCAAACGTCCAGTTATCATGCTGGGTAAAGGCGCTGCATACGCACAATGCGACGACGAAATCCGCGCACTGGTTGAAGAAACCGGCATCCCATTCCTGCCAATGGGTATGGCTAAAGGCCTGCTGCCTGACAACCATCCACAATCCGCTGCTGCAACCCGTGCTTTCGCACTGGCACAGTGTGACGTTTGCGTACTGATCGGCGCTCGTCTGAACTGGCTGATGCAGCACGGTAAAGGCAAAACCTGGGGCGACGAACTGAAGAAATACGTTCAGATCGACATCCAGGCTAACGAAATGGACAGCAACCAGCCTATCGCTGCACCAGTTGTTGGTGACATCAAGTCCGCCGTTTCCCTGCTCCGCAAAGCACTGAAAGGCGCTCCAAAAGCTGACGCTGAATGGACCGGCGCTCTGAAAGCCAAAGTTGACGGCAACAAAGCCAAACTGGCTGGCAAGATGACTGCCGAAACCCCATCCGGAATGATGAACTACTCCAATTCCCTGGGCGTTGTTCGTGACTTCATGCTGGCAAATCCGGATATTTCCCTGGTTAACGAAGGCGCTAATGCACTCGACAACACTCGTATGATTGTTGACATGCTGAAACCACGCAAACGTCTTGACTCCGGTACCTGGGGTGTTATGGGTATTGGTATGGGCTACTGCGTTGCTGCAGCTGCTGTTACCGGCAAACCGGTTATCGCTGTTGAAGGCGATAGCGCATTCGGTTTCTCCGGTATGGAACTGGAAACCATCTGCCGTTACAACCTGCCAGTTACCGTTATCATCATGAACAATGGTGGTATCTATAAAGGTAACGAAGCAGATCCACAACCAGGCGTTATCTCCTGTACCCGTCTGACCCGTGGTCGTTACGACATGATGATGGAAGCATTTGGCGGTAAAGGTTATGTTGCCAATACTCCAGCAGAACTGAAAGCTGCTCTGGAAGAAGCTGTTGCTTCCGGCAAACCATGCCTGATCAACGCGATGATCGATCCAGACGCTGGTGTCGAATCTGGCCGTATCAAGAGCCTGAACGTTGTAAGTAAAGTTGGCAAGAAATAAGGTTCCTAGCTGATTAGAAGGCCATCCTGACGGATGGCCTTTTTTTTGACTGCGTTCCTGGCGTGGTGCTCGCATAGTAGGATAAAACGAAAGGCTCAGTCGAAAGACTGGGCCTTTCGTTTTACAATTGGGCTACCTTTTTTTTGTTTTGTTTGCAATGGTTAATCTATTGTTAAAATTTAAAGTTTCACTTGAACTTTCAAATAATGTTCTTATATTTGCAGTGTCGAAAGAAACAAAGTAGCCTGGATCACACAACATTTAAAAAATAACATTATGAAAGCACTCGAAAAGAGATTCAAAGGTAATATTAACAATAATTTATTTTCAATGACAAGTGCCGCTACTGTTACTGCTTCGTTCAACGATACTTTTAGCGTATCCGATAATGTCGCCGTTATTGTTCCTGAAACGGACACTCAGGTGACCTACAGGGATCTATCCCACATGGTGGGTCACTTCCAGACCATGTTCACAAATCCTAATTCTCCATTGTACGGAGCTGTTTTCAGACAAGATACAGTGGCGATATCCATGCGTAATGGGCTGGAATTTATCGTCGCTTTCCTCGGTGCTACTATGGACGCTAAAATTGGCGCGCCCTTGAATCCCAATTATAAGGAAAAGGAGTTCAATTTTTATTTGAATGACCTGAAATCTAAGGCGATTTGCGTCCCAAAGGGTACCACAAAGTTACAGAGTTCTGAAATTCTAAAATCTGCCTCCACGTTTGGATGTTTTATCGTAGAGCTGGCCTTCGATGCGACCAGGTTTAGGGTAGAGTATGATATATACTCTCCAGAGGACAACTACAAAAGGGTTATTTACCGGTCTTTGAACAACGCCAAATTTGTCAACACAAATCCCGTTAAATTCCCTGGGTTTGCCCGTTCCAGTGACGTTGCCCTGATTTTGCATACCAGTGGTACCACCTCCACTCCAAAAACGGTGCCTTTGTTACATTTGAACATTGTGAGAAGCACGTTGAACATTGCTAACACTTACAAGCTAACGCCCTTGGACAGATCTTATGTCGTGATGCCTCTTTTCCACGTCCATGGGTTAATTGGTGTTTTACTTTCCACTTTTAGAACTCAGGGTTCTGTTGTGGTTCCCGATGGATTCCATCCAAAGTTATTCTGGGACCAATTTGTTAAGTACAACTGTAATTGGTTCAGTTGCGTTCCCACAATAAGCATGATTATGCTGAACATGCCCAAACCAAACCCTTTCCCACACATTAGATTCATCAGATCGTGTTCTTCTGCTTTGGCTCCAGCAACGTTCCATAAGCTGGAGAAGGAATTCAATGCACCTGTCTTGGAGGCCTATGCGATGACCGAAGCATCACATCAAATGACCTCAAACAATCTGCCTCCAGGAAAGAGAAAGCCTGGTACTGTGGGCCAGCCACAAGGAGTCACCGTCGTCATTCTAGATGACAATGACAATGTCTTGCCCCCGGGCAAAGTCGGCGAAGTTTCCATCAGAGGCGAAAACGTCACTTTGGGGTATGCTAATAATCCAAAAGCTAACAAGGAGAACTTCACCAAGAGAGAGAACTATTTCAGAACCGGTGACCAAGGTTATTTCGACCCTGAGGGGTTTTTGGTCCTTACAGGCAGAATCAAAGAGCTTATCAACAGGGGTGGTGAAAAGATTTCACCCATTGAGCTCGACGGCATTATGCTATCGCATCCAAAGATCGATGAAGCCGTTGCATTTGGTGTTCCCGACGATATGTACGGCCAAGTAGTTCAAGCCGCCATTGTTTTGAAGAAGGGAGAAAAAATGACCTACGAAGAACTGGTGAACTTCTTAAAGAAGCACCTAGCCTCTTTCAAAATTCCAACCAAGGTGTACTTTGTTGATAAGCTACCAAAAACCGCTACAGGTAAAATCCAGAGAAGAGTTATCGCAGAAACTTTTGCTAAGAGCAGCAGAAATAAGAGTAAGTTGTAGGGTTCCTAGCTGATTAGAAGGCCATCCTGACGGATGGCCTTTTTTTTGACTGCCCGACGAACGGACTAATCCTGACGTAGGATAAAACGAAAGGCTCAGTCGAAAGACTGGGCCTTTCGTTTTACAATTGGGCTACCTTTTTTTTGTTTTGTTTGCAATGGTTAATCTATTGTTAAAATTTAAAGTTTCACTTGAACTTTCAAATAATGTTCTTATATTTGCAGTGTCGAAAGAAACAAAGTAGCCTGGATCACACAACATTTAAAAAATAACATTATGAAAGCACTCGAAAAGAGATTCAAAGGTAATATTAACAATAATTTATTTTAATGAATAATCCACAAACAGGACAATCAACAGGCCTCTTGGGCAATCGTTGGTTCTACTTGGTATTAGCAGTTTTGCTGATGTGTATGATCTCGGGTGTCCAATATTCCTGGACACTGTACGCTAACCCGGTTAAAGACAACCTTGGCGTTTCTTTGGCTGCGGTTCAGACGGCTTTCACACTCTCTCAGGTCATTCAAGCTGGTTCTCAGCCTGGTGGTGGTTACTTCGTTGATAAATTCGGTCCAAGAATTCCATTGATGTTCGGTGGTGCGATGGTTCTCGCTGGCTGGACCTTCATGGGTATGGTTGACAGTGTTCCTGCTCTGTATGCTCTTTATACTCTGGCCGGTGCAGGTGTTGGTATCGTTTACGGTATCGCGATGAACACGGCTAACAGATGGTTCCCGGACAAACGCGGTCTGGCTTCCGGTTTCACCGCTGCCGGTTACGGTCTGGGTGTTCTGCCGTTCCTGCCACTGATCAGCTCCGTTCTGAAAGTTGAAGGTGTTGGCGCAGCATTCATGTACACCGGTTTGATCATGGGTATCCTGATTATCCTGATCGCTTTCGTTATCCGTTTCCCTGGCCAGCAAGGCGCCAAAAAACAAATCGTTGTTACCGACAAGGATTTCAATTCTGGCGAAATGCTGAGAACACCACAATTCTGGGTTCTGTGGACCGCATTCTTTTCCGTTAACTTTGGTGGTTTGCTGCTGGTTGCCAACAGCGTCCCTTACGGTCGCAGCCTCGGTCTTGCCGCAGGTGTGCTGACGATCGGTGTTTCGATCCAGAACCTGTTCAATGGTGGTTGCCGTCCTTTCTGGGGTTTCGTTTCCGATAAAATCGGCCGTTACAAAACCATGTCCGTCGTTTTCGGTATCAATGCTGTTGTTCTCGCACTTTTCCCGACGATTGCTGCCTTGGGCGATGTAGCCTTTATCGCCATGTTGGCAATCGCATTCTTCACATGGGGTGGTAGCTACGCTCTGTTCCCATCGACCAACAGCGATATTTTCGGTACGGCATACTCTGCCAGAAACTATGGTTTCTTCTGGGCTGCAAAAGCAACTGCCTCGATCTTCGGTGGTGGTCTGGGTGCTGCAATTGCAACCAACTTCGGATGGAATACCGCTTTCCTGATTACTGCGATTACTTCTTTCATCGCATTTGCTCTGGCTACCTTCGTTATTCCAAGAATGGGCCGTCCAGTCAAGAAAATGGTCAAATTGTCTCCAGAAGAAAAAGCTGTACATTAAGGTTCCTAGCTGATTAGAAGGCCATCCTGACGGATGGCCTTTTTTTTGACTGCTATGACTAAACGGCGCGCCTGATAGGTGGGCTGCCCTTCCTGGTTGGCTTGGTTTCATCAGCCATCCGCTTGCCCTCATCTGTTACGCCGGCGGTAGCCGGCCAGCCTCGCAGAGCAGGATTCCCGTTGAGCACCGCCAGGTGCGAATAAGGGACAGTGAAGAAGGAACACCCGCTCGCGGGTGGGCCTACTTCACCTATCCTGCCCGGCTGACGCCGTTGGATACACCAAGGAAAGTCTACACGAACCCTTTGGCAAAATCCTGTATATCGTGCGAAAAAGGATGGATATACCGAAAAAATCGCTATAATGACCCCGAAGCAGGGTTATGCAGCGGAAAAGCGGGATTAAAAGTCGGGGATTGGTGAACAAAAAGGTGTTTCTCTCTTTAAGAGAAATATCGTTTTGCTAAACAGTTGATATTGAGGTATCATTTTATCGTAAAAGACATTTTTGCTCAACAATTGCTTGACGGAAATCAACAAATTTTAGCATTTTGTAAAAAAGTCGCTATATAATTTGGTGAATTGGAGTTATTTTCATATTTTTGCATCCCGAAGAGTTTCTCTTAAAGAGAGAAACATCTTTTGCATACCTTTTCCGACCGAATTTTTATGTCGTAAAGAGGGGCTTTGCAGGGGGTGGACTCAGAAAGATGAGAATAGATGACTATTGTAGTTGAAACACATAGAAAGTTGCTGATATACAGACCGATACGCATATCGGGATGAACCATGAGTACGTTCTTTTCTCAAAAAACATAAATATTCGAAAAGAGATGCAATAAATTAAGGAGAGGTTATAATGAACAAAGTAAATATAAAAGATAGTCAAAATTTTATTACTTCAAAATATCACATAGAAAAAATAATGAATTGCATAAGTTTAGATGAAAAAGATAACATCTTTGAAATAGGTGCAGGGAAAGGTCATTTTACTGCTGGATTGGTAAAGAGATGTAATTTTGTAACGGCGATAGAAATTGATTCTAAATTATGTGAGGTAACTCGTAATAAGCTCTTAAATTATCCTAACTATCAAATAGTAAATGATGATATACTGAAATTTACATTTCCTAGCCACAATCCATATAAAATATTTGGCAGCATACCTTACAACATAAGCACAAATATAATTCGAAAAATTGTTTTTGAAAGTTCAGCCACAATAAGTTATTTAATAGTGGAATATGGTTTTGCTAAAATGTTATTAGATACAAACAGATCACTAGCATTGCTGTTAATGGCAGAGGTAGATATTTCTATATTAGCAAAAATTCCTAGGTATTATTTCCATCCAAAACCTAAAGTGGATAGCACATTAATTGTATTAAAAAGAAAGCCAGCAAAAATGGCATTTAAAGAGAGAAAAAAATATGAAACTTTTGTAATGAAATGGGTTAACAAAGAGTACGAAAAACTGTTTACAAAAAATCAATTTAATAAAGCTTTAAAACATGCGAGAATATATGATATAAACAATATTAGTTTCGAACAATTTGTATCGCTATTTAATAGTTATAAAATATTTAACGGCTAAAAACAATAGGCCACATGCAACTGTAAATGTTTACGCGGGTACCGACACCGCGGTGGAGGGGAATTACGAGTCATTGGTAACTATCTATGAAACTGTTTGATACTTTTATAGTTGATTAAACTTGTTCATGGCATTTGCCTTAATATCATCCGCTATGTCAATGTAGGGTTTCATAGCTTTGTAGTCGCTGTGTCCCGTCCATTTCATGACCACCTGTGCCGGGATTCCGAGAGCCAGCGCATTGCAGATGAATGTCCTTTTTCCTGCATGGGTACTGAGCAAAGCGTATTTGGGTGTGACTTCATCAATACGTTCATTTCCCTTGTAGTAGGTTTCCCGTACAGGCTCGTTGATTTCTGCCAGTTCGCCCAGCTCTTTCAGGTAATCGTTCATCTTCTGGTTGCTGATGACGGGCAGAGCCATGTAATTCTCGAAATGGATGTCCTTGTATTTGTCCAGTATGGCTTTGCTGTATTTGTTCAGTTCAATCGTCAGGCTGTCGGCAGTCTTGACTGTGGTTATTTCGATGTGGTCGGACTTCACATCGCTTCTTTTCAGATTGCGAACATCCGAATACCGCAAACTCGTAAAGCAGCAGAACAGGAAAACATCACGCACACGTTCCAGGTATTGCTTATCCTTGGGTATCTGGTAGTCTTTCAGCTTGTTCAGTTCATCCCAAGTCAGGAAGATTACTTTTTTCGAGGTGGTTTTCAGTTTCGGTTTGAACGTATCGTATGCAATGTTCTGATGATGTCCTTTCTTGAAGCTCCAGCGCAGGAACCATTTGAGGAATCCCATTTGCTTGCCGATGGTGCTGTTTCTCATATCCTTGGTGTCACGCAGGAAGTTGACGTATTCGTTCAATCCAAACTCGTTGAAATAGTTGAACGTTGCATCCTCCTTGAACTCTTTGAGGTGGTTCCTCACTGCTGCAAATTTTTCATAGGTGGATGCCGTCCAGTTATTCTGGTTACCGCACTCTTTTACAAACTCATCGAACACCTCCCAAAAGCTGACAGGGGCTTCTTCCGGCTGTTCTTCACTGGTATCTTTCATTCTCATGTTGAAAGCTTCCTTCAACTGTTGGGTCGTTGGCATGACCTCCTGCACCTCAAATTCCTTGAAAATATTCTGGATTTCGGCATAGTATTTCAGCAAGTCCGTATTGATTTCGGCTGCACTTTGCTTTAGCTTGTTGGTACATCCGTTCTTTACCCGCTGCTTATCTGCATCCCATTTGGCTACGTCAATCCGGTAGCCCGTTGTAAACTCGATACGTTGGCTGGCAAAGATGACACGCATACGGATGGGTACGTTCTCTACGATTGGCACACCGTTCTTTTTCCGGCTCTCCAATGCAAAAATGATGTTGCGCTTGATATTCATAATTGGGTGCGTTTGAAATTCTACACCCAAATATACACCCAATTATTGAGATAGCAAAAGACATTTAGAAACATTTACTTTTACTCTATATTGTAATTTACACTTGATTATCAGTCGTTTGCAGTCTTATGATATTCTGTGAAAGTATAAGTTCGAGAGCCTGTCTCTCCGCAAAAAACGCTGAAAATCAGCAGATTGCAAAACAAACACCCTGTTTTACACCCAAGAATGTAAAGTCGGGTGTTTTTGTTTTATTTAAGATAATACAACCACTACATAATAAAAGAGTAGCGATATTAAAAGAATCCGATGAGAAAAGACTAATATTTATCTATCCATTCAGTTTGATTTCTCAGGACTTTACATCGTCCTGAAAGTATTTGTTGCCAGTGTTACAACCAATTAACCAATTCTGATTAGAAAAACTCATCGAGCATCAAATGAAACTGCAATTTATTCATATCAGGATTATCAATACCATATTTTTGAAAAAGCCGTTTCTGTAATGAAGGAGAAAACTCACCGAGGCAGTTCCATAGGATGGCAAGATCCTGGTATCGGTCTGCGATTCCGACTCGTCCAACATCAATACAACCTATTAATTTCCCCTCGTCAAAAATAAGGTTATCAAGTGAGAAATCACCATGAGTGACGACTGAATCCGGTGAGAATGGCAAAAGCTTATGCATTTCTTTCCAGACTTGTTCAACAGGCCAGCCATTACGCTCGTCATCAAAATCACTCGCATCAACCAAACCGTTATTCATTCGTGATTGCGCCTGAGCGAGGCGAAATACGCGATCGCTGTTAAAAGGACAATTACAAACAGGAATCGAATGCAACCGGCGCAGGAACACTGCCAGCGCATCAACAATATTTTCACCTGAATCAGGATATTCTTCTAATACCTGGAATGCTGTTTTCCCGGGGATCGCAGTGGTGAGTAACCATGCATCATCAGGAGTACGGATAAAATGCTTGATGGTCGGAAGAGGCATAAATTCCGTCAGCCAGTTTAGTCTGACCATCTCATCTGTAACATCATTGGCAACGCTACCTTTGCCATGTTTCAGAAACAACTCTGGCGCATCGGGCTTCCCATACAATCGATAGATTGTCGCACCTGATTGCCCGACATTATCGCGAGCCCATTTATACCCATATAAATCAGCATCCATGTTGGAATTTAATCGCGGCCTGGAGCAAGACGTTTCCCGTTGAATATGGCTCATAACACCCCTTGTATTACTGTTTATGTAAGCAGACAGTTTTATTGTTCATGATGATATATTTTTATCTTGTGCAATGTAACATCAGAGATTTTGAGACACAACGTGGCTTTGTTGAATAAATCGAACTTTTGCTGAGTTGAAGGATCAGCCGCGCAGTTCAACCTGTTGATAGTACGTACTAAGCTCTCATGTTTCACGTACTAAGCTCTCATGTTTAACGTACTAAGCTCTCATGTTTAACGAACTAAACCCTCATGGCTAACGTACTAAGCTCTCATGGCTAACGTACTAAGCTCTCATGTTTCACGTACTAAGCTCTCATGTTTGAACAATAAAATTAATATAAATCAGCAACTTAAATAGCCTCTAAGGTTTTAAGTTTTATAAGAAAAAAAAGAATATATAAGGCTTTTAAAGCTTTTAAGGTTTAACGGTTGTGGACAACAAGCCAGGGATGTAACGCACTGAGAAGCCCTTAGAGCCTCTCAAAGCAATTTTGAGTGACACAGGAACACTTAACGGCTGACATGGGGCGGCCGCTCAAGTAG
SEQ ID NO:65(pZR761-Sc_YBR222C,Sc_YEL020C表达载体)：
GGAGAGCTGTATGTGGGTAGGATAAAACGAAAGGCTCAGTCGAAAGACTGGGCCTTTCGTTTTACAATTGGGCTACCTTTTTTTTGTTTTGTTTGCAATGGTTAATCTATTGTTAAAATTTAAAGTTTCACTTGAACTTTCAAATAATGTTCTTATATTTGCAGTGTCGAAAGAAACAAAGTAGCCTGGATCACACAACATTTAAAAAATAACATTATGAAAGCACTCGAAAAGAGATTCAAAGGTAATATTAACAATAATTTATTTTCAATGACAAGTGCCGCTACTGTTACTGCTTCGTTCAACGATACTTTTAGCGTATCCGATAATGTCGCCGTTATTGTTCCTGAAACGGACACTCAGGTGACCTACAGGGATCTATCCCACATGGTGGGTCACTTCCAGACCATGTTCACAAATCCTAATTCTCCATTGTACGGAGCTGTTTTCAGACAAGATACAGTGGCGATATCCATGCGTAATGGGCTGGAATTTATCGTCGCTTTCCTCGGTGCTACTATGGACGCTAAAATTGGCGCGCCCTTGAATCCCAATTATAAGGAAAAGGAGTTCAATTTTTATTTGAATGACCTGAAATCTAAGGCGATTTGCGTCCCAAAGGGTACCACAAAGTTACAGAGTTCTGAAATTCTAAAATCTGCCTCCACGTTTGGATGTTTTATCGTAGAGCTGGCCTTCGATGCGACCAGGTTTAGGGTAGAGTATGATATATACTCTCCAGAGGACAACTACAAAAGGGTTATTTACCGGTCTTTGAACAACGCCAAATTTGTCAACACAAATCCCGTTAAATTCCCTGGGTTTGCCCGTTCCAGTGACGTTGCCCTGATTTTGCATACCAGTGGTACCACCTCCACTCCAAAAACGGTGCCTTTGTTACATTTGAACATTGTGAGAAGCACGTTGAACATTGCTAACACTTACAAGCTAACGCCCTTGGACAGATCTTATGTCGTGATGCCTCTTTTCCACGTCCATGGGTTAATTGGTGTTTTACTTTCCACTTTTAGAACTCAGGGTTCTGTTGTGGTTCCCGATGGATTCCATCCAAAGTTATTCTGGGACCAATTTGTTAAGTACAACTGTAATTGGTTCAGTTGCGTTCCCACAATAAGCATGATTATGCTGAACATGCCCAAACCAAACCCTTTCCCACACATTAGATTCATCAGATCGTGTTCTTCTGCTTTGGCTCCAGCAACGTTCCATAAGCTGGAGAAGGAATTCAATGCACCTGTCTTGGAGGCCTATGCGATGACCGAAGCATCACATCAAATGACCTCAAACAATCTGCCTCCAGGAAAGAGAAAGCCTGGTACTGTGGGCCAGCCACAAGGAGTCACCGTCGTCATTCTAGATGACAATGACAATGTCTTGCCCCCGGGCAAAGTCGGCGAAGTTTCCATCAGAGGCGAAAACGTCACTTTGGGGTATGCTAATAATCCAAAAGCTAACAAGGAGAACTTCACCAAGAGAGAGAACTATTTCAGAACCGGTGACCAAGGTTATTTCGACCCTGAGGGGTTTTTGGTCCTTACAGGCAGAATCAAAGAGCTTATCAACAGGGGTGGTGAAAAGATTTCACCCATTGAGCTCGACGGCATTATGCTATCGCATCCAAAGATCGATGAAGCCGTTGCATTTGGTGTTCCCGACGATATGTACGGCCAAGTAGTTCAAGCCGCCATTGTTTTGAAGAAGGGAGAAAAAATGACCTACGAAGAACTGGTGAACTTCTTAAAGAAGCACCTAGCCTCTTTCAAAATTCCAACCAAGGTGTACTTTGTTGATAAGCTACCAAAAACCGCTACAGGTAAAATCCAGAGAAGAGTTATCGCAGAAACTTTTGCTAAGAGCAGCAGAAATAAGAGTAAGTTGTAGGGTTCCTAGCTGATTAGAAGGCCATCCTGACGGATGGCCTTTTTTTTGACTGGTAAGCCAATCTAGGGCCTCGTCTGTAGGATAAAACGAAAGGCTCAGTCGAAAGACTGGGCCTTTCGTTTTACAATTGGGCTACCTTTTTTTTGTTTTGTTTGCAATGGTTAATCTATTGTTAAAATTTAAAGTTTCACTTGAACTTTCAAATAATGTTCTTATATTTGCAGTGTCGAAAGAAACAAAGTAGCCTGGATCACACAACATTTAAAAAATAACATTATGAAAGCACTCGAAAAGAGATTCAAAGGTAATATTAACAATAATTTATTTTCAATGACCACGACCGCTACCCAACACTTCGCTCAACTTTTACAAAAATATGGCATCGACACAGTCTTTGGAATTGTGGGCATCCCTATTGTCCAACTAGCGGATACGATGGTTGCCAACGGTATCAAATTCATTCCATGCAGGAACGAACAGGCTGCCTCATATGCAGCCTCTGCTTATGGTTATATTAGTGATAAGCCCGGCGTGTTACTTATTGTCGGGGGCCCCGGTTTAATCCACGCACTGGCTGGTATATACAACTCAATGAGTAATAGGTGGCCTCTTCTAGTAATTGCAGGGAGTTCTTCTCAAAGTGATATACATAAAGGCGGGTTTCAAGAGTTGGATCAAGTTAGTCTTTTGTCCCCATTTTTGAAGTTTACCGGAAAGTTAACCCCTGACAATATTGACATGATTACTCAGAAGGCTTTGAATTACTGTATACAAGGCACTGCAGGGGTTTCTTACATAGACGTCCCTGCAGATTTTATTGAGTATGAAAAACCCTTAGAAGGAAACGACCGTACAGGAAATGAATTACCGATGATATTAACTCCAAACATATGTGGCCCTGATCCGTCGAAGATCAAAAAAGTCGTACAGCTTATTTTGCAACATAAAAATAAAAATATTCTGATTGTTATTGGAAAGGGTGCGGTAAAAAACTCACACGAAATCCGCAGACTAGTAAATACGTTCAATCTACCATTTTTACCCACCCCAATGGCTAAGGGAATTGTCCCAGACTCTTCTCCACTGAACGTTTCATCTGCAAGATCTCAAGCATTGAAAATAGCGGATATTGTTCTTGTTCTCGGGGCAAGATTAAACTGGATATTGCATTTTGGTACTTCACCCAAATGGAACTCAGAATCCATATTCATCCAGTTCGATTCCAATCCCGAAACCTTGGGGGATAACAATGTCTCACCTGGTGCTGACCTTTCCATATGGGGAGATATAGGCTTGAGCGTAACCGCTTTAGTTGAGGAATTGACTCGCCAAGATTCCTGCTGGAAGTATAGTGGTGTTAAGCAAGAAATACGAGAGAAAATTCAACTGAATCAAACTCGTCTATTGAGGAAAGAGAAGACTAGAGGGGCACAATTGAATTACAACCAGGTTTATGGAACATTGAGGCCTCTTATCGATGATTACAGGACAATACTTGTGACGGAGGGAGCAAATACTATGGATATTGCGCGTATTTCATTCCCTACAGACGCTCCAAGGCGCCGTTTAGACGCAGGGACCAACGCGACTATGGGGATTGGGCTCGGATATGCTCTTGCATGCAAGGCATCTCATCCGGAACTCGATGTGGTGCTGATTCAGGGTGATTCCGCATTTGGATTCTCTGCCATGGAAATTGAAACGGCAGTAAGGTGTCAGCTGGCATTGGTCATCGTTGTGATGAACAATAGCGGTATTTACCATGGAGAAAAGGATATAGAGGGTGATTTACCTCCAACGGCACTAAGCAAGAACTGCCGTTACGATCTCGTAGGCAAAGGACTGGGTGCCAATGATTTTTTTGTCAACACAATAAGCGAACTGAGCAGATGCTTCCAACAAGCCGTGCAGCTTTCTCGGACTAAAAGAGAAACAAGTGTCATCAACGTTATCATTGAGCCTGGCGAACAAAAGCAAATTGCCTTTGCCTGGCAGAATAAACCGCGTTTATAGGGTTCCTAGCTGATTAGAAGGCCATCCTGACGGATGGCCTTTTTTTTGACTGCGTTCCTGGCGTGGTGCTCGCATAGTAGACTAGAGATCTATGTGAGGATCCTAACTCGAGATCGAGACTGCTATGACTAAACGGCGCGCCTGATAGGTGGGCTGCCCTTCCTGGTTGGCTTGGTTTCATCAGCCATCCGCTTGCCCTCATCTGTTACGCCGGCGGTAGCCGGCCAGCCTCGCAGAGCAGGATTCCCGTTGAGCACCGCCAGGTGCGAATAAGGGACAGTGAAGAAGGAACACCCGCTCGCGGGTGGGCCTACTTCACCTATCCTGCCCGGCTGACGCCGTTGGATACACCAAGGAAAGTCTACACGAACCCTTTGGCAAAATCCTGTATATCGTGCGAAAAAGGATGGATATACCGAAAAAATCGCTATAATGACCCCGAAGCAGGGTTATGCAGCGGAAAAGTTATATACATTCATGTCCATTTATGTAAAAAATCCTGCTGACCTTGTTTATGTCTTGTCAGTCACCATTTGCAAAACCATATTTGACCCTCAAAGAGGCTGAATTTGATAAGCAACTTGCTACATACTCATAATAAGGAGCTAAATAGAACACGAATGGGAAATACTCAAATGCCAAACTAAAGAAGATATTGGCCAAAATAAACGCTATACCGAGAGAGAAACTTGATTTTTCAACTTCCTAAAACAGTGTTGTTCAAACATTTCTACTTATTTGTACTTACCAGTTGAACCTACGTTTCCCTAATAAAATGTCTATGGTAAAAAGTTAAAAAATCCTCCTACTTTTGTTAGATATATTTTTTTGTGTAATTTTGTAATCGTTATGCGGCAGTAATAATATACATATTAATACGAGTTAGGAATCCTGTAGTTCTCATATGCTACGAGGAGGTATTAAAAGGTGCGTTTCGACAATGCATCTATTGTAGTATATTATTGCTTAATCCAAATGAATATTATAAATTTAGGAATTCTTGCTCACATTGATGCAGGAAAAACTTCCGTAACCGAGAATCTGCTGTTTGCCAGTGGAGCAACGGAAAAGTGCGGCTGTGTGGATAATGGTGACACCATAACGGACTCTATGGATATAGAGAAACGTAGAGGAATTACTGTTCGGGCTTCTACGACATCTATTATCTGGAATGGTGTGAAATGCAATATCATTGACACTCCGGGACACATGGATTTTATTGCGGAAGTGGAGCGGACATTCAAAATGCTTGATGGAGCAGTCCTCATCTTATCCGCAAAGGAAGGCATACAAGCGCAGACAAAGTTGCTGTTCAATACTTTACAGAAGCTGCAAATCCCGACAATTATATTTATCAATAAGATTGACCGAGCCGGTGTGAATTTGGAGCGTTTGTATCTGGATATAAAAGCAAATCTGTCTCAAGATGTCCTGTTTATGCAAAATGTTGTCGATGGATCGGTTTATCCGGTTTGCTCCCAAACATATATAAAGGAAGAATACAAAGAATTTGTATGCAACCATGACGACAATATATTAGAACGATATTTGGCGGATAGCGAAATTTCACCGGCTGATTATTGGAATACGATAATCGCTCTTGTGGCAAAAGCCAAAGTCTATCCGGTGCTACATGGATCAGCAATGTTCAATATCGGTATCAATGAGTTGTTGGACGCCATCACTTCTTTTATACTTCCTCCGGCATCGGTTTCAAACAGACTTTCATCTTATCTTTATAAGATAGAGCATGACCCCAAAGGACATAAAAGAAGTTTTCTAAAAATAATTGACGGAAGTCTGAGACTTCGAGATGTTGTAAGAATCAACGATTCGGAAAAATTCATCAAGATTAAAAATCTAAAAACTATCAATCAGGGCAGAGAGATAAATGTTGATGAAGTGGGCGCCAATGATATCGCGATTGTAGAGGATATGGATGATTTTCGAATCGGAAATTATTTAGGTGCTGAACCTTGTTTGATTCAAGGATTATCGCATCAGCATCCCGCTCTCAAATCCTCCGTCCGGCCAGACAGGCCCGAAGAGAGAAGCAAGGTGATATCCGCTCTGAATACATTGTGGATTGAAGATCCGTCTTTGTCCTTTTCCATAAACTCATATAGTGATGAATTGGAAATCTCGTTATATGGTTTAACCCAAAAGGAAATCATACAGACATTGCTGGAAGAACGATTTTCCGTAAAGGTCCATTTTGATGAGATCAAGACTATATACAAAGAACGACCTGTAAAAAAGGTCAATAAGATTATTCAGATCGAAGTGCCGCCCAACCCTTATTGGGCCACAATAGGGCTGACTCTTGAACCCTTACCGTTAGGGACAGGGTTGCAAATCGAAAGTGACATCTCCTATGGTTATCTGAACCATTCTTTTCAAAATGCCGTTTTTGAAGGGATTCGTATGTCTTGCCAATCCGGGTTACATGGATGGGAAGTGACTGATCTGAAAGTAACTTTTACTCAAGCCGAGTATTATAGCCCGGTAAGTACACCAGCTGATTTCAGACAGCTGACCCCTTATGTCTTTAGGCTGGCCTTGCAACAGTCAGGTGTGGACATTCTCGAACCGATGCTCTATTTTGAGTTGCAGATACCCCAAGCGGCAAGTTCCAAAGCTATTACAGATTTGCAAAAAATGATGTCTGAGATTGAAGATATCAGTTGCAATAATGAGTGGTGTCATATTAAAGGGAAAGTTCCATTAAATACAAGTAAAGACTATGCATCAGAAGTAAGTTCATACACTAAGGGCTTAGGCATTTTTATGGTTAAGCCATGCGGGTATCAAATAACAAAAGGCGGTTATTCTGATAATATCCGCATGAACGAAAAAGATAAACTTTTATTCATGTTCCAAAAATCAATGTCATCAAAATAACCACGAGTCATTGGTAACTATCTATGAAACTGTTTGATACTTTTATAGTTGATTAAACTTGTTCATGGCATTTGCCTTAATATCATCCGCTATGTCAATGTAGGGTTTCATAGCTTTGTAGTCGCTGTGTCCCGTCCATTTCATGACCACCTGTGCCGGGATTCCGAGAGCCAGCGCATTGCAGATGAATGTCCTTTTTCCTGCATGGGTACTGAGCAAAGCGTATTTGGGTGTGACTTCATCAATACGTTCATTTCCCTTGTAGTAGGTTTCCCGTACAGGCTCGTTGATTTCTGCCAGTTCGCCCAGCTCTTTCAGGTAATCGTTCATCTTCTGGTTGCTGATGACGGGCAGAGCCATGTAATTCTCGAAATGGATGTCCTTGTATTTGTCCAGTATGGCTTTGCTGTATTTGTTCAGTTCAATCGTCAGGCTGTCGGCAGTCTTGACTGTGGTTATTTCGATGTGGTCGGACTTCACATCGCTTCTTTTCAGATTGCGAACATCCGAATACCGCAAACTCGTAAAGCAGCAGAACAGGAAAACATCACGCACACGTTCCAGGTATTGCTTATCCTTGGGTATCTGGTAGTCTTTCAGCTTGTTCAGTTCATCCCAAGTCAGGAAGATTACTTTTTTCGAGGTGGTTTTCAGTTTCGGTTTGAACGTATCGTATGCAATGTTCTGATGATGTCCTTTCTTGAAGCTCCAGCGCAGGAACCATTTGAGGAATCCCATTTGCTTGCCGATGGTGCTGTTTCTCATATCCTTGGTGTCACGCAGGAAGTTGACGTATTCGTTCAATCCAAACTCGTTGAAATAGTTGAACGTTGCATCCTCCTTGAACTCTTTGAGGTGGTTCCTCACTGCTGCAAATTTTTCATAGGTGGATGCCGTCCAGTTATTCTGGTTACCGCACTCTTTTACAAACTCATCGAACACCTCCCAAAAGCTGACAGGGGCTTCTTCCGGCTGTTCTTCACTGGTATCTTTCATTCTCATGTTGAAAGCTTCCTTCAACTGTTGGGTCGTTGGCATGACCTCCTGCACCTCAAATTCCTTGAAAATATTCTGGATTTCGGCATAGTATTTCAGCAAGTCCGTATTGATTTCGGCTGCACTTTGCTTTAGCTTGTTGGTACATCCGTTCTTTACCCGCTGCTTATCTGCATCCCATTTGGCTACGTCAATCCGGTAGCCCGTTGTAAACTCGATACGTTGGCTGGCAAAGATGACACGCATACGGATGGGTACGTTCTCTACGATTGGCACACCGTTCTTTTTCCGGCTCTCCAATGCAAAAATGATGTTGCGCTTGATATTCATAATTGGGTGCGTTTGAAATTCTACACCCAAATATACACCCAATTATTGAGATAGCAAAAGACATTTAGAAACATTTACTTTTACTCTATATTGTAATTTACACTTGATTATCAGTCGTTTGCAGTCTTATGATATTCTGTGAAAGTATAAGTTCGAGAGCCTGTCTCTCCGCAAAAAACGCTGAAAATCAGCAGATTGCAAAACAAACACCCTGTTTTACACCCAAGAATGTAAAGTCGGGTGTTTTTGTTTTATTTAAGATAATACAACCACTACATAATAAAAGAGTAGCGATATTAAAAGAATCCGATGAGAAAAGACTAATATTTATCTATCCATTCAGTTTGATTTCTCAGGACTTTACATCGTCCTGAAAGTATTTGTTGCCAGTGTTACAACCAATTAACCAATTCTGATTAGAAAAACTCATCGAGCATCAAATGAAACTGCAATTTATTCATATCAGGATTATCAATACCATATTTTTGAAAAAGCCGTTTCTGTAATGAAGGAGAAAACTCACCGAGGCAGTTCCATAGGATGGCAAGATCCTGGTATCGGTCTGCGATTCCGACTCGTCCAACATCAATACAACCTATTAATTTCCCCTCGTCAAAAATAAGGTTATCAAGTGAGAAATCACCATGAGTGACGACTGAATCCGGTGAGAATGGCAAAAGCTTATGCATTTCTTTCCAGACTTGTTCAACAGGCCAGCCATTACGCTCGTCATCAAAATCACTCGCATCAACCAAACCGTTATTCATTCGTGATTGCGCCTGAGCGAGGCGAAATACGCGATCGCTGTTAAAAGGACAATTACAAACAGGAATCGAATGCAACCGGCGCAGGAACACTGCCAGCGCATCAACAATATTTTCACCTGAATCAGGATATTCTTCTAATACCTGGAATGCTGTTTTCCCGGGGATCGCAGTGGTGAGTAACCATGCATCATCAGGAGTACGGATAAAATGCTTGATGGTCGGAAGAGGCATAAATTCCGTCAGCCAGTTTAGTCTGACCATCTCATCTGTAACATCATTGGCAACGCTACCTTTGCCATGTTTCAGAAACAACTCTGGCGCATCGGGCTTCCCATACAATCGATAGATTGTCGCACCTGATTGCCCGACATTATCGCGAGCCCATTTATACCCATATAAATCAGCATCCATGTTGGAATTTAATCGCGGCCTGGAGCAAGACGTTTCCCGTTGAATATGGCTCATAACACCCCTTGTATTACTGTTTATGTAAGCAGACAGTTTTATTGTTCATGATGATATATTTTTATCTTGTGCAATGTAACATCAGAGATTTTGAGACACAACGTGGCTTTGTTGAATAAATCGAACTTTTGCTGAGTTGAAGGATCAGCCGCGCAGTTCAACCTGTTGATAGTACGTACTAAGCTCTCATGTTTCACGTACTAAGCTCTCATGTTTAACGTACTAAGCTCTCATGTTTAACGAACTAAACCCTCATGGCTAACGTACTAAGCTCTCATGGCTAACGTACTAAGCTCTCATGTTTCACGTACTAAGCTCTCATGTTTGAACAATAAAATTAATATAAATCAGCAACTTAAATAGCCTCTAAGGTTTTAAGTTTTATAAGAAAAAAAAGAATATATAAGGCTTTTAAAGCTTTTAAGGTTTAACGGTTGTGGACAACAAGCCAGGGATGTAACGCACTGAGAAGCCCTTAGAGCCTCTCAAAGCAATTTTGAGTGACACAGGAACACTTAACGGCTGACATGGGGCGGCCGCTCAAGTAG
SEQ ID NO:66(pZR856-Of_oxlT表达载体)：
GATAAAACGAAAGGCTCAGTCGAAAGACTGGGCCTTTCGTTTTACAATTGGGCTACCTTTTTTTTGTTTTGTTTGCAATGGTTAATCTATTGTTGAAATTTAAAGTTTCACTTGAACTTTCAAATAATGTTCTTATATTTGCAGTGTCGAAAGAAACAAAGTAGCCTGGATCACACAACATTTAAAAAATAACATTATGAAAGCACTCGAAAAGAGATTCAAAGGTAATATTAACAATAATTTATTTTAATGAATAATCCACAAACAGGACAATCAACAGGCCTCTTGGGCAATCGTTGGTTCTACTTGGTATTAGCAGTTTTGCTGATGTGTATGATCTCGGGTGTCCAATATTCCTGGACACTGTACGCTAACCCGGTTAAAGACAACCTTGGCGTTTCTTTGGCTGCGGTTCAGACGGCTTTCACACTCTCTCAGGTCATTCAAGCTGGTTCTCAGCCTGGTGGTGGTTACTTCGTTGATAAATTCGGTCCAAGAATTCCATTGATGTTCGGTGGTGCGATGGTTCTCGCTGGCTGGACCTTCATGGGTATGGTTGACAGTGTTCCTGCTCTGTATGCTCTTTATACTCTGGCCGGTGCAGGTGTTGGTATCGTTTACGGTATCGCGATGAACACGGCTAACAGATGGTTCCCGGACAAACGCGGTCTGGCTTCCGGTTTCACCGCTGCCGGTTACGGTCTGGGTGTTCTGCCGTTCCTGCCACTGATCAGCTCCGTTCTGAAAGTTGAAGGTGTTGGCGCAGCATTCATGTACACCGGTTTGATCATGGGTATCCTGATTATCCTGATCGCTTTCGTTATCCGTTTCCCTGGCCAGCAAGGCGCCAAAAAACAAATCGTTGTTACCGACAAGGATTTCAATTCTGGCGAAATGCTGAGAACACCACAATTCTGGGTTCTGTGGACCGCATTCTTTTCCGTTAACTTTGGTGGTTTGCTGCTGGTTGCCAACAGCGTCCCTTACGGTCGCAGCCTCGGTCTTGCCGCAGGTGTGCTGACGATCGGTGTTTCGATCCAGAACCTGTTCAATGGTGGTTGCCGTCCTTTCTGGGGTTTCGTTTCCGATAAAATCGGCCGTTACAAAACCATGTCCGTCGTTTTCGGTATCAATGCTGTTGTTCTCGCACTTTTCCCGACGATTGCTGCCTTGGGCGATGTAGCCTTTATCGCCATGTTGGCAATCGCATTCTTCACATGGGGTGGTAGCTACGCTCTGTTCCCATCGACCAACAGCGATATTTTCGGTACGGCATACTCTGCCAGAAACTATGGTTTCTTCTGGGCTGCAAAAGCAACTGCCTCGATCTTCGGTGGTGGTCTGGGTGCTGCAATTGCAACCAACTTCGGATGGAATACCGCTTTCCTGATTACTGCGATTACTTCTTTCATCGCATTTGCTCTGGCTACCTTCGTTATTCCAAGAATGGGCCGTCCAGTCAAGAAAATGGTCAAATTGTCTCCAGAAGAAAAAGCTGTACATTAAGGTTCCTAGCTGATTAGAAGGCCATCCTGACGGATGGCCTTTTTTTTGACTGCTATGACTTGAGACCGGCTATTACGAGCGCTTAAACGGCGCGCCTGATAGGTGGGCTGCCCTTCCTGGTTGGCTTGGTTTCATCAGCCATCCGCTTGCCCTCATCTGTTACGCCGGCGGTAGCCGGCCAGCCTCGCAGAGCAGGATTCCCGTTGAGCACCGCCAGGTGCGAATAAGGGACAGTGAAGAAGGAACACCCGCTCGCGGGTGGGCCTACTTCACCTATCCTGCCCGGCTGACGCCGTTGGATACACCAAGGAAAGTCTACACGAACCCTTTGGCAAAATCCTGTATATCGTGCGAAAAAGGATGGATATACCGAAAAAATCGCTATAATGACCCCGAAGCAGGGTTATGCAGCGGAAAAGTTATATACATTCATGTCCATTTATGTAAAAAATCCTGCTGACCTTGTTTATGTCTTGTCAGTCACCATTTGCAAAACCATATTTGACCCTCAAAGAGGCTGAATTTGATAAGCAACTTGCTACATACTCATAATAAGGAGCTAAATAGAACACGAATGGGAAATACTCAAATGCCAAACTAAAGAAGATATTGGCCAAAATAAACGCTATACCGAGAGAGAAACTTGATTTTTCAACTTCCTAAAACAGTGTTGTTCAAACATTTCTACTTATTTGTACTTACCAGTTGAACCTACGTTTCCCTAATAAAATGTCTATGGTAAAAAGTTAAAAAATCCTCCTACTTTTGTTAGATATATTTTTTTGTGTAATTTTGTAATCGTTATGCGGCAGTAATAATATACATATTAATACGAGTTAGGAATCCTGTAGTTCTCATATGCTACGAGGAGGTATTAAAAGGTGCGTTTCGACAATGCATCTATTGTAGTATATTATTGCTTAATCCAAATGAATATTATAAATTTAGGAATTCTTGCTCACATTGATGCAGGAAAAACTTCCGTAACCGAGAATCTGCTGTTTGCCAGTGGAGCAACGGAAAAGTGCGGCTGTGTGGATAATGGTGACACCATAACGGACTCTATGGATATAGAGAAACGTAGAGGAATTACTGTTCGGGCTTCTACGACATCTATTATCTGGAATGGTGTGAAATGCAATATCATTGACACTCCGGGACACATGGATTTTATTGCGGAAGTGGAGCGGACATTCAAAATGCTTGATGGAGCAGTCCTCATCTTATCCGCAAAGGAAGGCATACAAGCGCAGACAAAGTTGCTGTTCAATACTTTACAGAAGCTGCAAATCCCGACAATTATATTTATCAATAAGATTGACCGAGCCGGTGTGAATTTGGAGCGTTTGTATCTGGATATAAAAGCAAATCTGTCTCAAGATGTCCTGTTTATGCAAAATGTTGTCGATGGATCGGTTTATCCGGTTTGCTCCCAAACATATATAAAGGAAGAATACAAAGAATTTGTATGCAACCATGACGACAATATATTAGAACGATATTTGGCGGATAGCGAAATTTCACCGGCTGATTATTGGAATACGATAATCGCTCTTGTGGCAAAAGCCAAAGTCTATCCGGTGCTACATGGATCAGCAATGTTCAATATCGGTATCAATGAGTTGTTGGACGCCATCACTTCTTTTATACTTCCTCCGGCATCGGTTTCAAACAGACTTTCATCTTATCTTTATAAGATAGAGCATGACCCCAAAGGACATAAAAGAAGTTTTCTAAAAATAATTGACGGAAGTCTGAGACTTCGAGATGTTGTAAGAATCAACGATTCGGAAAAATTCATCAAGATTAAAAATCTAAAAACTATCAATCAGGGCAGAGAGATAAATGTTGATGAAGTGGGCGCCAATGATATCGCGATTGTAGAGGATATGGATGATTTTCGAATCGGAAATTATTTAGGTGCTGAACCTTGTTTGATTCAAGGATTATCGCATCAGCATCCCGCTCTCAAATCCTCCGTCCGGCCAGACAGGCCCGAAGAGAGAAGCAAGGTGATATCCGCTCTGAATACATTGTGGATTGAAGATCCGTCTTTGTCCTTTTCCATAAACTCATATAGTGATGAATTGGAAATCTCGTTATATGGTTTAACCCAAAAGGAAATCATACAGACATTGCTGGAAGAACGATTTTCCGTAAAGGTCCATTTTGATGAGATCAAGACTATATACAAAGAACGACCTGTAAAAAAGGTCAATAAGATTATTCAGATCGAAGTGCCGCCCAACCCTTATTGGGCCACAATAGGGCTGACTCTTGAACCCTTACCGTTAGGGACAGGGTTGCAAATCGAAAGTGACATCTCCTATGGTTATCTGAACCATTCTTTTCAAAATGCCGTTTTTGAAGGGATTCGTATGTCTTGCCAATCCGGGTTACATGGATGGGAAGTGACTGATCTGAAAGTAACTTTTACTCAAGCCGAGTATTATAGCCCGGTAAGTACACCAGCTGATTTCAGACAGCTGACCCCTTATGTCTTTAGGCTGGCCTTGCAACAGTCAGGTGTGGACATTCTCGAACCGATGCTCTATTTTGAGTTGCAGATACCCCAAGCGGCAAGTTCCAAAGCTATTACAGATTTGCAAAAAATGATGTCTGAGATTGAAGATATCAGTTGCAATAATGAGTGGTGTCATATTAAAGGGAAAGTTCCATTAAATACAAGTAAAGACTATGCATCAGAAGTAAGTTCATACACTAAGGGCTTAGGCATTTTTATGGTTAAGCCATGCGGGTATCAAATAACAAAAGGCGGTTATTCTGATAATATCCGCATGAACGAAAAAGATAAACTTTTATTCATGTTCCAAAAATCAATGTCATCAAAATAACCACGAGTCATTGGTAACTATCTATGAAACTGTTTGATACTTTTATAGTTGATTAAACTTGTTCATGGCATTTGCCTTAATATCATCCGCTATGTCAATGTAGGGTTTCATAGCTTTGTAGTCGCTGTGTCCCGTCCATTTCATGACCACCTGTGCCGGGATTCCGAGAGCCAGCGCATTGCAGATGAATGTCCTTTTTCCTGCATGGGTACTGAGCAAAGCGTATTTGGGTGTGACTTCATCAATACGTTCATTTCCCTTGTAGTAGGTTTCCCGTACAGGCTCGTTGATTTCTGCCAGTTCGCCCAGCTCTTTCAGGTAATCGTTCATCTTCTGGTTGCTGATGACGGGCAGAGCCATGTAATTCTCGAAATGGATGTCCTTGTATTTGTCCAGTATGGCTTTGCTGTATTTGTTCAGTTCAATCGTCAGGCTGTCGGCAGTCTTGACTGTGGTTATTTCGATGTGGTCGGACTTCACATCGCTTCTTTTCAGATTGCGAACATCCGAATACCGCAAACTCGTAAAGCAGCAGAACAGGAAAACATCACGCACACGTTCCAGGTATTGCTTATCCTTGGGTATCTGGTAGTCTTTCAGCTTGTTCAGTTCATCCCAAGTCAGGAAGATTACTTTTTTCGAGGTGGTTTTCAGTTTCGGTTTGAACGTATCGTATGCAATGTTCTGATGATGTCCTTTCTTGAAGCTCCAGCGCAGGAACCATTTGAGGAATCCCATTTGCTTGCCGATGGTGCTGTTTCTCATATCCTTGGTGTCACGCAGGAAGTTGACGTATTCGTTCAATCCAAACTCGTTGAAATAGTTGAACGTTGCATCCTCCTTGAACTCTTTGAGGTGGTTCCTCACTGCTGCAAATTTTTCATAGGTGGATGCCGTCCAGTTATTCTGGTTACCGCACTCTTTTACAAACTCATCGAACACCTCCCAAAAGCTGACAGGGGCTTCTTCCGGCTGTTCTTCACTGGTATCTTTCATTCTCATGTTGAAAGCTTCCTTCAACTGTTGGGTCGTTGGCATGACCTCCTGCACCTCAAATTCCTTGAAAATATTCTGGATTTCGGCATAGTATTTCAGCAAGTCCGTATTGATTTCGGCTGCACTTTGCTTTAGCTTGTTGGTACATCCGTTCTTTACCCGCTGCTTATCTGCATCCCATTTGGCTACGTCAATCCGGTAGCCCGTTGTAAACTCGATACGTTGGCTGGCAAAGATGACACGCATACGGATGGGTACGTTCTCTACGATTGGCACACCGTTCTTTTTCCGGCTCTCCAATGCAAAAATGATGTTGCGCTTGATATTCATAATTGGGTGCGTTTGAAATTCTACACCCAAATATACACCCAATTATTGAGATAGCAAAAGACATTTAGAAACATTTACTTTTACTCTATATTGTAATTTACACTTGATTATCAGTCGTTTGCAGTCTTATGATATTCTGTGAAAGTATAAGTTCGAGAGCCTGTCTCTCCGCAAAAAACGCTGAAAATCAGCAGATTGCAAAACAAACACCCTGTTTTACACCCAAGAATGTAAAGTCGGGTGTTTTTGTTTTATTTAAGATAATACAACCACTACATAATAAAAGAGTAGCGATATTAAAAGAATCCGATGAGAAAAGACTAATATTTATCTATCCATTCAGTTTGATTTCTCAGGACTTTACATCGTCCTGAAAGTATTTGTTGCCAGTGTTACAACCAATTAACCAATTCTGATTAGAAAAACTCATCGAGCATCAAATGAAACTGCAATTTATTCATATCAGGATTATCAATACCATATTTTTGAAAAAGCCGTTTCTGTAATGAAGGAGAAAACTCACCGAGGCAGTTCCATAGGATGGCAAGATCCTGGTATCGGTCTGCGATTCCGACTCGTCCAACATCAATACAACCTATTAATTTCCCCTCGTCAAAAATAAGGTTATCAAGTGAGAAATCACCATGAGTGACGACTGAATCCGGTGAGAATGGCAAAAGCTTATGCATTTCTTTCCAGACTTGTTCAACAGGCCAGCCATTACGCTCGTCATCAAAATCACTCGCATCAACCAAACCGTTATTCATTCGTGATTGCGCCTGAGCGAGGCGAAATACGCGATCGCTGTTAAAAGGACAATTACAAACAGGAATCGAATGCAACCGGCGCAGGAACACTGCCAGCGCATCAACAATATTTTCACCTGAATCAGGATATTCTTCTAATACCTGGAATGCTGTTTTCCCGGGGATCGCAGTGGTGAGTAACCATGCATCATCAGGAGTACGGATAAAATGCTTGATGGTCGGAAGAGGCATAAATTCCGTCAGCCAGTTTAGTCTGACCATCTCATCTGTAACATCATTGGCAACGCTACCTTTGCCATGTTTCAGAAACAACTCTGGCGCATCGGGCTTCCCATACAATCGATAGATTGTCGCACCTGATTGCCCGACATTATCGCGAGCCCATTTATACCCATATAAATCAGCATCCATGTTGGAATTTAATCGCGGCCTGGAGCAAGACGTTTCCCGTTGAATATGGCTCATAACACCCCTTGTATTACTGTTTATGTAAGCAGACAGTTTTATTGTTCATGATGATATATTTTTATCTTGTGCAATGTAACATCAGAGATTTTGAGACACAACGTGGCTTTGTTGAATAAATCGAACTTTTGCTGAGTTGAAGGATCAGCCGCGCAGTTCAACCTGTTGATAGTACGTACTAAGCTCTCATGTTTCACGTACTAAGCTCTCATGTTTAACGTACTAAGCTCTCATGTTTAACGAACTAAACCCTCATGGCTAACGTACTAAGCTCTCATGGCTAACGTACTAAGCTCTCATGTTTCACGTACTAAGCTCTCATGTTTGAACAATAAAATTAATATAAATCAGCAACTTAAATAGCCTCTAAGGTTTTAAGTTTTATAAGAAAAAAAAGAATATATAAGGCTTTTAAAGCTTTTAAGGTTTAACGGTTGTGGACAACAAGCCAGGGATGTAACGCACTGAGAAGCCCTTAGAGCCTCTCAAAGCAATTTTGAGTGACACAGGAACACTTAACGGCTGACATGGGGCGGCCGCTCAACGTACCGGTCTCAGTAGGGAGAGCTGTATGTGGGTAG
SEQ ID NO:67(pZR878-Ec_frc,Of_oxc,Sc_YBR222C,Sc_YEL020C,At_AAE3,Ec_yfdE表达载体)：
GGAGAGCTGTATGTGGGTAGGATAAAACGAAAGGCTCAGTCGAAAGACTGGGCCTTTCGTTTTACAATTGGGCTACCTTTTTTTTGTTTTGTTTGCAATGGTTAATCTATTGTTAAAATTTAAAGTTTCACTTGAACTTTCAAATAATGTTCTTATATTTGCAGTGTCGAAAGAAACAAAGTAGCCTGGATCACACAACATTTAAAAAATAACATTATGAAAGCACTCGAAAAGAGATTCAAAGGTAATATTAACAATAATTTATTTTCAATGTCAACTCCACTTCAAGGAATTAAAGTTCTCGATTTCACCGGTGTGCAATCTGGCCCATCTTGTACTCAAATGCTGGCCTGGTTTGGCGCTGACGTTATTAAAATTGAACGTCCCGGCGTTGGTGACGTAACGCGTCACCAGCTGCGAGATATTCCTGATATCGATGCGCTTTACTTCACCATGCTTAACAGTAACAAACGTTCTATTGAGTTAAATACCAAAACAGCGGAAGGCAAAGAGGTAATGGAAAAGCTGATCCGCGAAGCTGATATCTTAGTCGAGAACTTTCATCCAGGGGCCATTGATCACATGGGCTTCACCTGGGAGCATATTCAAGAAATCAATCCACGTCTGATTTTTGGTTCGATCAAAGGGTTTGATGAGTGTTCGCCTTATGTGAATGTAAAAGCCTATGAAAACGTTGCTCAGGCAGCGGGTGGCGCGGCATCCACTACGGGTTTTTGGGATGGTCCGCCGCTGGTAAGCGCTGCAGCGTTGGGTGACAGCAACACCGGAATGCATTTGCTGATCGGTTTACTTGCTGCTTTGCTGCATCGCGAAAAAACGGGGCGTGGGCAACGAGTCACCATGTCAATGCAGGATGCCGTATTGAACCTTTGCCGCGTGAAATTACGTGACCAGCAGCGTCTAGATAAATTGGGTTATCTGGAAGAATACCCGCAGTATCCGAATGGTACATTTGGTGATGCAGTTCCCCGCGGTGGTAATGCAGGTGGTGGCGGTCAGCCTGGCTGGATCCTGAAATGTAAAGGCTGGGAAACCGATCCTAACGCCTATATTTATTTCACTATTCAGGAGCAAAACTGGGAAAACACCTGTAAAGCCATCGGCAAACCAGAATGGATTACCGATCCGGCATACAGTACAGCCCATGCACGACAGCCACATATTTTCGATATTTTTGCTGAAATCGAAAAATACACTGTCACTATTGATAAACATGAAGCGGTGGCCTATTTGACTCAGTTTGATATTCCTTGTGCACCGGTTTTAAGTATGAAAGAAATTTCACTTGATCCCTCTTTGCGCCAAAGTGGCAGTGTTGTTGAAGTGGAACAACCGTTGCGTGGAAAATATCTGACCGTTGGTTGTCCAATGAAATTCTCTGCCTTTACGCCGGATATTAAAGCTGCGCCGCTATTAGGTGAACATACCGCTGCTGTATTGCAGGAGCTGGGTTATAGCGACGATGAAATTGCTGCAATGAAGCAAAACCACGCCATCTGAGGTTCCTAGCTGATTAGAAGGCCATCCTGACGGATGGCCTTTTTTTTGACTGGTAAGCCAATCTAGGGCCTCGTCTGTAGGATAAAACGAAAGGCTCAGTCGAAAGACTGGGCCTTTCGTTTTACAATTGGGCTACCTTTTTTTTGTTTTGTTTGCAATGGTTAATCTATTGTTAAAATTTAAAGTTTCACTTGAACTTTCAAATAATGTTCTTATATTTGCAGTGTCGAAAGAAACAAAGTAGCCTGAGCTGTCACCGGATGTGCTTTCCGGTCTGATGAGTCCGTGAGGACGAAACAGCCTCTACAAATAATTTTGTTTAATCCATCAATTTAAAATTTAAAATAATGGTTTTTACTCTGGAAGATTTTGTTGGCGATTGGCGTCAGACCGCGGGTTATAATTTGGATCAAGTCCTGGAACAGGGTGGCGTAAGCTCTCTGTTCCAGAACCTGGGTGTGAGCGTGACGCCGATTCAGCGCATCGTTCTGTCCGGCGAGAACGGTCTGAAAATTGATATTCATGTGATCATCCCGTACGAAGGCCTGAGCGGTGACCAAATGGGTCAAATCGAGAAAATCTTTAAAGTCGTCTACCCAGTTGACGATCACCACTTCAAGGTTATCTTGCATTACGGTACGCTGGTGATTGATGGTGTGACCCCGAATATGATTGACTATTTCGGCCGTCCGTATGAAGGCATTGCCGTTTTTGACGGTAAAAAGATCACCGTCACCGGTACCCTGTGGAATGGCAATAAGATTATTGACGAGCGTCTGATTAACCCGGACGGCAGCCTGCTGTTCCGCGTGACCATCAACGGTGTCACGGGTTGGCGTCTGTGCGAGCGCATCCTGGCAGGTTCAATGAGTAACGACGACAATGTAGAGTTGACTGATGGCTTTCATGTTTTGATCGATGCCCTGAAAATGAATGACATCGATACCATGTATGGTGTTGTCGGCATTCCTATCACGAACCTGGCTCGTATGTGGCAAGATGACGGTCAGCGTTTTTACAGCTTCCGTCACGAACAACACGCAGGTTATGCAGCTTCTATCGCCGGTTACATCGAAGGAAAACCTGGCGTTTGCTTGACCGTTTCCGCCCCTGGCTTCCTGAACGGCGTGACTTCCCTGGCTCATGCAACCACCAACTGCTTCCCAATGATCCTGTTGAGCGGTTCCAGTGAACGTGAAATCGTCGATTTGCAACAGGGCGATTACGAAGAAATGGATCAGATGAATGTTGCACGTCCACACTGCAAAGCTTCTTTCCGTATCAACAGCATCAAAGACATTCCAATCGGTATCGCTCGTGCAGTTCGCACCGCTGTATCCGGACGTCCAGGTGGTGTTTACGTTGACTTGCCAGCAAAACTGTTCGGTCAGACCATTTCTGTAGAAGAAGCTAACAAACTGCTCTTCAAACCAATCGATCCAGCTCCGGCACAGATTCCTGCTGAAGATGCTATCGCTCGCGCTGCTGACCTGATCAAGAACGCCAAACGTCCAGTTATCATGCTGGGTAAAGGCGCTGCATACGCACAATGCGACGACGAAATCCGCGCACTGGTTGAAGAAACCGGCATCCCATTCCTGCCAATGGGTATGGCTAAAGGCCTGCTGCCTGACAACCATCCACAATCCGCTGCTGCAACCCGTGCTTTCGCACTGGCACAGTGTGACGTTTGCGTACTGATCGGCGCTCGTCTGAACTGGCTGATGCAGCACGGTAAAGGCAAAACCTGGGGCGACGAACTGAAGAAATACGTTCAGATCGACATCCAGGCTAACGAAATGGACAGCAACCAGCCTATCGCTGCACCAGTTGTTGGTGACATCAAGTCCGCCGTTTCCCTGCTCCGCAAAGCACTGAAAGGCGCTCCAAAAGCTGACGCTGAATGGACCGGCGCTCTGAAAGCCAAAGTTGACGGCAACAAAGCCAAACTGGCTGGCAAGATGACTGCCGAAACCCCATCCGGAATGATGAACTACTCCAATTCCCTGGGCGTTGTTCGTGACTTCATGCTGGCAAATCCGGATATTTCCCTGGTTAACGAAGGCGCTAATGCACTCGACAACACTCGTATGATTGTTGACATGCTGAAACCACGCAAACGTCTTGACTCCGGTACCTGGGGTGTTATGGGTATTGGTATGGGCTACTGCGTTGCTGCAGCTGCTGTTACCGGCAAACCGGTTATCGCTGTTGAAGGCGATAGCGCATTCGGTTTCTCCGGTATGGAACTGGAAACCATCTGCCGTTACAACCTGCCAGTTACCGTTATCATCATGAACAATGGTGGTATCTATAAAGGTAACGAAGCAGATCCACAACCAGGCGTTATCTCCTGTACCCGTCTGACCCGTGGTCGTTACGACATGATGATGGAAGCATTTGGCGGTAAAGGTTATGTTGCCAATACTCCAGCAGAACTGAAAGCTGCTCTGGAAGAAGCTGTTGCTTCCGGCAAACCATGCCTGATCAACGCGATGATCGATCCAGACGCTGGTGTCGAATCTGGCCGTATCAAGAGCCTGAACGTTGTAAGTAAAGTTGGCAAGAAATAAGGTTCCTAGCTGATTAGAAGGCCATCCTGACGGATGGCCTTTTTTTTGACTGCGTTCCTGGCGTGGTGCTCGCATAGTAGGATAAAACGAAAGGCTCAGTCGAAAGACTGGGCCTTTCGTTTTACAATTGGGCTACCTTTTTTTTGTTTTGTTTGCAATGGTTAATCTATTGTTAAAATTTAAAGTTTCACTTGAACTTTCAAATAATGTTCTTATATTTGCAGTGTCGAAAGAAACAAAGTAGCCTGGATCACACAACATTTAAAAAATAACATTATGAAAGCACTCGAAAAGAGATTCAAAGGTAATATTAACAATAATTTATTTTCAATGACAAGTGCCGCTACTGTTACTGCTTCGTTCAACGATACTTTTAGCGTATCCGATAATGTCGCCGTTATTGTTCCTGAAACGGACACTCAGGTGACCTACAGGGATCTATCCCACATGGTGGGTCACTTCCAGACCATGTTCACAAATCCTAATTCTCCATTGTACGGAGCTGTTTTCAGACAAGATACAGTGGCGATATCCATGCGTAATGGGCTGGAATTTATCGTCGCTTTCCTCGGTGCTACTATGGACGCTAAAATTGGCGCGCCCTTGAATCCCAATTATAAGGAAAAGGAGTTCAATTTTTATTTGAATGACCTGAAATCTAAGGCGATTTGCGTCCCAAAGGGTACCACAAAGTTACAGAGTTCTGAAATTCTAAAATCTGCCTCCACGTTTGGATGTTTTATCGTAGAGCTGGCCTTCGATGCGACCAGGTTTAGGGTAGAGTATGATATATACTCTCCAGAGGACAACTACAAAAGGGTTATTTACCGGTCTTTGAACAACGCCAAATTTGTCAACACAAATCCCGTTAAATTCCCTGGGTTTGCCCGTTCCAGTGACGTTGCCCTGATTTTGCATACCAGTGGTACCACCTCCACTCCAAAAACGGTGCCTTTGTTACATTTGAACATTGTGAGAAGCACGTTGAACATTGCTAACACTTACAAGCTAACGCCCTTGGACAGATCTTATGTCGTGATGCCTCTTTTCCACGTCCATGGGTTAATTGGTGTTTTACTTTCCACTTTTAGAACTCAGGGTTCTGTTGTGGTTCCCGATGGATTCCATCCAAAGTTATTCTGGGACCAATTTGTTAAGTACAACTGTAATTGGTTCAGTTGCGTTCCCACAATAAGCATGATTATGCTGAACATGCCCAAACCAAACCCTTTCCCACACATTAGATTCATCAGATCGTGTTCTTCTGCTTTGGCTCCAGCAACGTTCCATAAGCTGGAGAAGGAATTCAATGCACCTGTCTTGGAGGCCTATGCGATGACCGAAGCATCACATCAAATGACCTCAAACAATCTGCCTCCAGGAAAGAGAAAGCCTGGTACTGTGGGCCAGCCACAAGGAGTCACCGTCGTCATTCTAGATGACAATGACAATGTCTTGCCCCCGGGCAAAGTCGGCGAAGTTTCCATCAGAGGCGAAAACGTCACTTTGGGGTATGCTAATAATCCAAAAGCTAACAAGGAGAACTTCACCAAGAGAGAGAACTATTTCAGAACCGGTGACCAAGGTTATTTCGACCCTGAGGGGTTTTTGGTCCTTACAGGCAGAATCAAAGAGCTTATCAACAGGGGTGGTGAAAAGATTTCACCCATTGAGCTCGACGGCATTATGCTATCGCATCCAAAGATCGATGAAGCCGTTGCATTTGGTGTTCCCGACGATATGTACGGCCAAGTAGTTCAAGCCGCCATTGTTTTGAAGAAGGGAGAAAAAATGACCTACGAAGAACTGGTGAACTTCTTAAAGAAGCACCTAGCCTCTTTCAAAATTCCAACCAAGGTGTACTTTGTTGATAAGCTACCAAAAACCGCTACAGGTAAAATCCAGAGAAGAGTTATCGCAGAAACTTTTGCTAAGAGCAGCAGAAATAAGAGTAAGTTGTAGGGTTCCTAGCTGATTAGAAGGCCATCCTGACGGATGGCCTTTTTTTTGACTGCCCGACGAACGGACTAATCCTGACGTAGGATAAAACGAAAGGCTCAGTCGAAAGACTGGGCCTTTCGTTTTACAATTGGGCTACCTTTTTTTTGTTTTGTTTGCAATGGTTAATCTATTGTTAAAATTTAAAGTTTCACTTGAACTTTCAAATAATGTTCTTATATTTGCAGTGTCGAAAGAAACAAAGTAGCCTGGATCACACAACATTTAAAAAATAACATTATGAAAGCACTCGAAAAGAGATTCAAAGGTAATATTAACAATAATTTATTTTCAATGACCACGACCGCTACCCAACACTTCGCTCAACTTTTACAAAAATATGGCATCGACACAGTCTTTGGAATTGTGGGCATCCCTATTGTCCAACTAGCGGATACGATGGTTGCCAACGGTATCAAATTCATTCCATGCAGGAACGAACAGGCTGCCTCATATGCAGCCTCTGCTTATGGTTATATTAGTGATAAGCCCGGCGTGTTACTTATTGTCGGGGGCCCCGGTTTAATCCACGCACTGGCTGGTATATACAACTCAATGAGTAATAGGTGGCCTCTTCTAGTAATTGCAGGGAGTTCTTCTCAAAGTGATATACATAAAGGCGGGTTTCAAGAGTTGGATCAAGTTAGTCTTTTGTCCCCATTTTTGAAGTTTACCGGAAAGTTAACCCCTGACAATATTGACATGATTACTCAGAAGGCTTTGAATTACTGTATACAAGGCACTGCAGGGGTTTCTTACATAGACGTCCCTGCAGATTTTATTGAGTATGAAAAACCCTTAGAAGGAAACGACCGTACAGGAAATGAATTACCGATGATATTAACTCCAAACATATGTGGCCCTGATCCGTCGAAGATCAAAAAAGTCGTACAGCTTATTTTGCAACATAAAAATAAAAATATTCTGATTGTTATTGGAAAGGGTGCGGTAAAAAACTCACACGAAATCCGCAGACTAGTAAATACGTTCAATCTACCATTTTTACCCACCCCAATGGCTAAGGGAATTGTCCCAGACTCTTCTCCACTGAACGTTTCATCTGCAAGATCTCAAGCATTGAAAATAGCGGATATTGTTCTTGTTCTCGGGGCAAGATTAAACTGGATATTGCATTTTGGTACTTCACCCAAATGGAACTCAGAATCCATATTCATCCAGTTCGATTCCAATCCCGAAACCTTGGGGGATAACAATGTCTCACCTGGTGCTGACCTTTCCATATGGGGAGATATAGGCTTGAGCGTAACCGCTTTAGTTGAGGAATTGACTCGCCAAGATTCCTGCTGGAAGTATAGTGGTGTTAAGCAAGAAATACGAGAGAAAATTCAACTGAATCAAACTCGTCTATTGAGGAAAGAGAAGACTAGAGGGGCACAATTGAATTACAACCAGGTTTATGGAACATTGAGGCCTCTTATCGATGATTACAGGACAATACTTGTGACGGAGGGAGCAAATACTATGGATATTGCGCGTATTTCATTCCCTACAGACGCTCCAAGGCGCCGTTTAGACGCAGGGACCAACGCGACTATGGGGATTGGGCTCGGATATGCTCTTGCATGCAAGGCATCTCATCCGGAACTCGATGTGGTGCTGATTCAGGGTGATTCCGCATTTGGATTCTCTGCCATGGAAATTGAAACGGCAGTAAGGTGTCAGCTGGCATTGGTCATCGTTGTGATGAACAATAGCGGTATTTACCATGGAGAAAAGGATATAGAGGGTGATTTACCTCCAACGGCACTAAGCAAGAACTGCCGTTACGATCTCGTAGGCAAAGGACTGGGTGCCAATGATTTTTTTGTCAACACAATAAGCGAACTGAGCAGATGCTTCCAACAAGCCGTGCAGCTTTCTCGGACTAAAAGAGAAACAAGTGTCATCAACGTTATCATTGAGCCTGGCGAACAAAAGCAAATTGCCTTTGCCTGGCAGAATAAACCGCGTTTATAGGGTTCCTAGCTGATTAGAAGGCCATCCTGACGGATGGCCTTTTTTTTGACTGCTCTGGGTCTGACGGCCATCAAGGGTAGGATAAAACGAAAGGCTCAGTCGAAAGACTGGGCCTTTCGTTTTACAATTGGGCTACCTTTTTTTTGTTTTGTTTGCAATGGTTAATCTATTGTTAAAATTTAAAGTTTCACTTGAACTTTCAAATAATGTTCTTATATTTGCAGTGTCGAAAGAAACAAAGTAGCCTGGATCACACAACATTTAAAAAATAACATTATGAAAGCACTCGAAAAGAGATTCAAAGGTAATATTAACAATAATTTATTTTCAATGGATAGCGATACTCTCTCAGGATTATTGGAAAACGTCGCCAAAAAATTCCCCGATCGCCGAGCTCTCTCCGTTTCTGGAAAATTCAATCTCACTCACGCGCGTCTTCACGATCTAATCGAACGCGCCGCTTCACGCCTTGTCTCCGACGCTGGAATCAAACCCGGCGATGTCGTTGCTCTCACCTTCCCTAACACCGTCGAGTTTGTTATAATGTTTTTAGCGGTGATAAGAGCTAGAGCCACGGCGGCGCCGTTGAACGCAGCGTACACGGCGGAGGAATTTGAGTTTTACCTCTCCGATTCAGATTCAAAGCTATTGTTAACCTCTAAAGAAGGAAACGCACCGGCTCAAGAAGCAGCTTCAAAGCTGAAAATCTCTCACGTCACCGCTACGCTGCTTGACGCTGGCTCGGACCTTGTACTATCCGTTGCGGATTCAGATTCCGTCGTTGACTCAGCGACGGAACTCGTTAATCACCCGGACGACGGTGCTCTCTTCCTCCACACTTCTGGCACTACGAGCCGTCCAAAGGGTGTACCGCTTACGCAGCTCAATCTAGCTTCATCCGTCAAGAACATTAAAGCTGTGTACAAGCTTACTGAGTCTGATTCTACGGTGATTGTTCTCCCTCTGTTCCATGTTCATGGATTGTTAGCTGGGTTGCTTAGCTCGCTTGGAGCTGGTGCTGCTGTAACTCTTCCAGCTGCTGGTAGATTCTCAGCAACAACATTTTGGCCAGATATGAAGAAGTATAACGCTACATGGTATACTGCTGTGCCGACCATTCATCAGATCATATTGGACCGCCACGCGAGCCACCCTGAAACGGAATATCCTAAACTCCGGTTCATCAGGAGTTGCAGTGCTTCTTTGGCTCCGGTGATATTGTCCAGGCTTGAGGAAGCGTTTGGAGCACCGGTGCTCGAGGCCTATGCAATGACAGAGGCAACACATTTGATGAGCTCAAACCCCTTACCAGAGGAAGGTCCACACAAGCCTGGGTCTGTTGGGAAACCGGTAGGTCAAGAAATGGCGATCCTTAATGAGAAAGGCGAGATCCAAGAGCCAAATAACAAAGGAGAGGTTTGTATAAGAGGTCCAAATGTGACCAAGGGTTACAAGAATAACCCAGAAGCCAACAAGGCAGGTTTCGAGTTTGGGTGGTTCCACACTGGTGATATCGGTTACTTTGATACCGATGGGTATTTGCATCTGGTGGGTCGGATCAAAGAGCTTATTAACCGTGGAGGTGAGAAGATATCTCCAATTGAAGTGGATGCAGTACTCTTAACGCATCCTGACGTTTCTCAGGGTGTTGCATTCGGTGTTCCTGATGAGAAATATGGGGAAGAGATTAACTGTGCGGTGATTCCAAGAGAAGGAACTACTGTAACCGAAGAGGACATTAAAGCGTTTTGTAAGAAGAATTTGGCAGCTTTCAAGGTGCCAAAGAGAGTGTTCATCACTGATAACCTCCCCAAAACTGCCTCTGGTAAGATTCAGCGCCGTATCGTCGCACAACATTTCCTTGAGAAGCCCTGAGGTTCCTAGCTGATTAGAAGGCCATCCTGACGGATGGCCTTTTTTTTGACTGGGTGTTCCGATACACTCAATTTGTGTAGGATAAAACGAAAGGCTCAGTCGAAAGACTGGGCCTTTCGTTTTACAATTGGGCTACCTTTTTTTTGTTTTGTTTGCAATGGTTAATCTATTGTTAAAATTTAAAGTTTCACTTGAACTTTCAAATAATGTTCTTATATTTGCAGTGTCGAAAGAAACAAAGTAGCCTGGATCACACAACATTTAAAAAATAACATTATGAAAGCACTCGAAAAGAGATTCAAAGGTAATATTAACAATAATTTATTTTCAATGACAAATAATGAAAGCAAAGGGCCGTTTGAAGGCTTATTAGTTATCGATATGACACATGTCCTTAATGGACCTTTCGGAACTCAACTTCTTTGTAATATGGGCGCAAGGGTAATTAAAGTTGAGCCGCCGGGTCATGGTGATGATACCCGCACATTTGGTCCCTATGTGGATGGACAGTCACTCTATTACAGTTTTATTAATCATGGCAAAGAGAGTGTGGTTCTTGATTTAAAGAATGATCACGATAAAAGTATATTTATAAATATGCTCAAACAAGCTGATGTATTAGCTGAGAATTTTCGCCCAGGTACAATGGAAAAACTGGGGTTTTCATGGGAAACGCTTCAAGAAATCAACCCGCGCCTCATATATGCTTCATCGTCAGGTTTCGGACATACCGGTCCGCTAAAAGATGCTCCTGCCTACGATACCATCATTCAGGCAATGAGCGGGATAATGATGGAAACAGGATATCCTGATGCTCCGCCAGTGCGCGTTGGTACATCTCTTGCGGATCTATGCGGCGGTGTCTATTTATTCAGCGGAATAGTGAGTGCACTTTATGGCCGCGAAAAGAGCCAGAGAGGGGCGCATGTCGATATAGCGATGTTTGATGCCACGCTGAGTTTTCTGGAGCATGGTCTGATGGCATATATCGCAACTGGGAAGTCACCACAACGTCTGGGAAATCGCCATCCCTACATGGCACCTTTTGATGTTTTCAATACTCAGGATAAGCCGATTACGATTTGTTGTGGTAATGACAAGCTTTTTTCTGCGTTATGCCAGGCACTGGAGCTTACGGAACTGGTTAATGATCCCCGATTTAGCAGCAATATTTTACGCGTACAAAACCAGGCTATTCTTAAACAATATATTGAGCGGACGTTAAAAACGCAGGCAGCTGAAGTTTGGTTAGCCAGAATACATGAAGTTGGTGTACCCGTCGCGCCGTTATTAAGTGTGGCTGAGGCCATTAAATTGCCACAAACTCAGGCGAGAAATATGTTGATTGAAGCCGGGGGAATAATGATGCCGGGTAATCCGATAAAAATCAGCGGCTGCGCGGACCCGCATGTTATGCCGGGAGCGGCAACGCTCGACCAGCATGGGGAACAAATTCGCCAGGAGTTCTCATCATAAGGTTCCTAGCTGATTAGAAGGCCATCCTGACGGATGGCCTTTTTTTTGACTTAGTTTCAATTTTACATTCCAGGAAGTAGACTAGAGATCTATGTGAGGATCCTAACTCGAGATCGAGACTGCTATGACTAAACGGCGCGCCTGATAGGTGGGCTGCCCTTCCTGGTTGGCTTGGTTTCATCAGCCATCCGCTTGCCCTCATCTGTTACGCCGGCGGTAGCCGGCCAGCCTCGCAGAGCAGGATTCCCGTTGAGCACCGCCAGGTGCGAATAAGGGACAGTGAAGAAGGAACACCCGCTCGCGGGTGGGCCTACTTCACCTATCCTGCCCGGCTGACGCCGTTGGATACACCAAGGAAAGTCTACACGAACCCTTTGGCAAAATCCTGTATATCGTGCGAAAAAGGATGGATATACCGAAAAAATCGCTATAATGACCCCGAAGCAGGGTTATGCAGCGGAAAAGCGGGATTAAAAGTCGGGGATTGGTGAACAAAAAGGTGTTTCTCTCTTTAAGAGAAATATCGTTTTGCTAAACAGTTGATATTGAGGTATCATTTTATCGTAAAAGACATTTTTGCTCAACAATTGCTTGACGGAAATCAACAAATTTTAGCATTTTGTAAAAAAGTCGCTATATAATTTGGTGAATTGGAGTTATTTTCATATTTTTGCATCCCGAAGAGTTTCTCTTAAAGAGAGAAACATCTTTTGCATACCTTTTCCGACCGAATTTTTATGTCGTAAAGAGGGGCTTTGCAGGGGGTGGACTCAGAAAGATGAGAATAGATGACTATTGTAGTTGAAACACATAGAAAGTTGCTGATATACAGACCGATACGCATATCGGGATGAACCATGAGTACGTTCTTTTCTCAAAAAACATAAATATTCGAAAAGAGATGCAATAAATTAAGGAGAGGTTATAATGAACAAAGTAAATATAAAAGATAGTCAAAATTTTATTACTTCAAAATATCACATAGAAAAAATAATGAATTGCATAAGTTTAGATGAAAAAGATAACATCTTTGAAATAGGTGCAGGGAAAGGTCATTTTACTGCTGGATTGGTAAAGAGATGTAATTTTGTAACGGCGATAGAAATTGATTCTAAATTATGTGAGGTAACTCGTAATAAGCTCTTAAATTATCCTAACTATCAAATAGTAAATGATGATATACTGAAATTTACATTTCCTAGCCACAATCCATATAAAATATTTGGCAGCATACCTTACAACATAAGCACAAATATAATTCGAAAAATTGTTTTTGAAAGTTCAGCCACAATAAGTTATTTAATAGTGGAATATGGTTTTGCTAAAATGTTATTAGATACAAACAGATCACTAGCATTGCTGTTAATGGCAGAGGTAGATATTTCTATATTAGCAAAAATTCCTAGGTATTATTTCCATCCAAAACCTAAAGTGGATAGCACATTAATTGTATTAAAAAGAAAGCCAGCAAAAATGGCATTTAAAGAGAGAAAAAAATATGAAACTTTTGTAATGAAATGGGTTAACAAAGAGTACGAAAAACTGTTTACAAAAAATCAATTTAATAAAGCTTTAAAACATGCGAGAATATATGATATAAACAATATTAGTTTCGAACAATTTGTATCGCTATTTAATAGTTATAAAATATTTAACGGCTAAAAACAATAGGCCACATGCAACTGTAAATGTTTACGCGGGTACCGACACCGCGGTGGAGGGGAATTACGAGTCATTGGTAACTATCTATGAAACTGTTTGATACTTTTATAGTTGATTAAACTTGTTCATGGCATTTGCCTTAATATCATCCGCTATGTCAATGTAGGGTTTCATAGCTTTGTAGTCGCTGTGTCCCGTCCATTTCATGACCACCTGTGCCGGGATTCCGAGAGCCAGCGCATTGCAGATGAATGTCCTTTTTCCTGCATGGGTACTGAGCAAAGCGTATTTGGGTGTGACTTCATCAATACGTTCATTTCCCTTGTAGTAGGTTTCCCGTACAGGCTCGTTGATTTCTGCCAGTTCGCCCAGCTCTTTCAGGTAATCGTTCATCTTCTGGTTGCTGATGACGGGCAGAGCCATGTAATTCTCGAAATGGATGTCCTTGTATTTGTCCAGTATGGCTTTGCTGTATTTGTTCAGTTCAATCGTCAGGCTGTCGGCAGTCTTGACTGTGGTTATTTCGATGTGGTCGGACTTCACATCGCTTCTTTTCAGATTGCGAACATCCGAATACCGCAAACTCGTAAAGCAGCAGAACAGGAAAACATCACGCACACGTTCCAGGTATTGCTTATCCTTGGGTATCTGGTAGTCTTTCAGCTTGTTCAGTTCATCCCAAGTCAGGAAGATTACTTTTTTCGAGGTGGTTTTCAGTTTCGGTTTGAACGTATCGTATGCAATGTTCTGATGATGTCCTTTCTTGAAGCTCCAGCGCAGGAACCATTTGAGGAATCCCATTTGCTTGCCGATGGTGCTGTTTCTCATATCCTTGGTGTCACGCAGGAAGTTGACGTATTCGTTCAATCCAAACTCGTTGAAATAGTTGAACGTTGCATCCTCCTTGAACTCTTTGAGGTGGTTCCTCACTGCTGCAAATTTTTCATAGGTGGATGCCGTCCAGTTATTCTGGTTACCGCACTCTTTTACAAACTCATCGAACACCTCCCAAAAGCTGACAGGGGCTTCTTCCGGCTGTTCTTCACTGGTATCTTTCATTCTCATGTTGAAAGCTTCCTTCAACTGTTGGGTCGTTGGCATGACCTCCTGCACCTCAAATTCCTTGAAAATATTCTGGATTTCGGCATAGTATTTCAGCAAGTCCGTATTGATTTCGGCTGCACTTTGCTTTAGCTTGTTGGTACATCCGTTCTTTACCCGCTGCTTATCTGCATCCCATTTGGCTACGTCAATCCGGTAGCCCGTTGTAAACTCGATACGTTGGCTGGCAAAGATGACACGCATACGGATGGGTACGTTCTCTACGATTGGCACACCGTTCTTTTTCCGGCTCTCCAATGCAAAAATGATGTTGCGCTTGATATTCATAATTGGGTGCGTTTGAAATTCTACACCCAAATATACACCCAATTATTGAGATAGCAAAAGACATTTAGAAACATTTACTTTTACTCTATATTGTAATTTACACTTGATTATCAGTCGTTTGCAGTCTTATGATATTCTGTGAAAGTATAAGTTCGAGAGCCTGTCTCTCCGCAAAAAACGCTGAAAATCAGCAGATTGCAAAACAAACACCCTGTTTTACACCCAAGAATGTAAAGTCGGGTGTTTTTGTTTTATTTAAGATAATACAACCACTACATAATAAAAGAGTAGCGATATTAAAAGAATCCGATGAGAAAAGACTAATATTTATCTATCCATTCAGTTTGATTTCTCAGGACTTTACATCGTCCTGAAAGTATTTGTTGCCAGTGTTACAACCAATTAACCAATTCTGATTAGAAAAACTCATCGAGCATCAAATGAAACTGCAATTTATTCATATCAGGATTATCAATACCATATTTTTGAAAAAGCCGTTTCTGTAATGAAGGAGAAAACTCACCGAGGCAGTTCCATAGGATGGCAAGATCCTGGTATCGGTCTGCGATTCCGACTCGTCCAACATCAATACAACCTATTAATTTCCCCTCGTCAAAAATAAGGTTATCAAGTGAGAAATCACCATGAGTGACGACTGAATCCGGTGAGAATGGCAAAAGCTTATGCATTTCTTTCCAGACTTGTTCAACAGGCCAGCCATTACGCTCGTCATCAAAATCACTCGCATCAACCAAACCGTTATTCATTCGTGATTGCGCCTGAGCGAGGCGAAATACGCGATCGCTGTTAAAAGGACAATTACAAACAGGAATCGAATGCAACCGGCGCAGGAACACTGCCAGCGCATCAACAATATTTTCACCTGAATCAGGATATTCTTCTAATACCTGGAATGCTGTTTTCCCGGGGATCGCAGTGGTGAGTAACCATGCATCATCAGGAGTACGGATAAAATGCTTGATGGTCGGAAGAGGCATAAATTCCGTCAGCCAGTTTAGTCTGACCATCTCATCTGTAACATCATTGGCAACGCTACCTTTGCCATGTTTCAGAAACAACTCTGGCGCATCGGGCTTCCCATACAATCGATAGATTGTCGCACCTGATTGCCCGACATTATCGCGAGCCCATTTATACCCATATAAATCAGCATCCATGTTGGAATTTAATCGCGGCCTGGAGCAAGACGTTTCCCGTTGAATATGGCTCATAACACCCCTTGTATTACTGTTTATGTAAGCAGACAGTTTTATTGTTCATGATGATATATTTTTATCTTGTGCAATGTAACATCAGAGATTTTGAGACACAACGTGGCTTTGTTGAATAAATCGAACTTTTGCTGAGTTGAAGGATCAGCCGCGCAGTTCAACCTGTTGATAGTACGTACTAAGCTCTCATGTTTCACGTACTAAGCTCTCATGTTTAACGTACTAAGCTCTCATGTTTAACGAACTAAACCCTCATGGCTAACGTACTAAGCTCTCATGGCTAACGTACTAAGCTCTCATGTTTCACGTACTAAGCTCTCATGTTTGAACAATAAAATTAATATAAATCAGCAACTTAAATAGCCTCTAAGGTTTTAAGTTTTATAAGAAAAAAAAGAATATATAAGGCTTTTAAAGCTTTTAAGGTTTAACGGTTGTGGACAACAAGCCAGGGATGTAACGCACTGAGAAGCCCTTAGAGCCTCTCAAAGCAATTTTGAGTGACACAGGAACACTTAACGGCTGACATGGGGCGGCCGCTCAAGTAG
SEQ ID NO:68(P_BfP1E6)：
GATAAAACGAAAGGCTCAGTCGAAAGACTGGGCCTTTCGTTTTACAATTGGGCTACCTTTTTTTTGTTTTGTTTGCAATGGTTAATCTATTGTTAAAATTTAAAGTTTCACTTGAACTTTCAAATAATGTTCTTATATTTGCAGTGTCGAAAGAAACAAAGTAG
SEQ ID NO:69(P_BfP4E5)：
GATAAAACGAAAGGCTCAGTCGAAAGACTGGGCCTTTCGTTTTACAATTGGGCTACCTTTTTTTTGTTTTGTTTGCAATGGTTAATCTATTGTTGAAATTTAAAGTTTCACTTGAACTTTCAAATAATGTTCTTATATTTGCAGTGTCGAAAGAAACAAAGTAG
SEQ ID NO:70(噬菌体启动子共有序列)：
GTTAA(n)4-7GTTAA(n)34-38TA(n)2TTTG
SEQ ID NO:71(噬菌体启动子共有序列)：
GTTAAnnnnnnnGTTAAnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnTAnnTTTG
SEQ ID NO:72(NB001紫菜多糖PUL)：
TAAGGATTGATTCGCTAGCTCAGCAGGTAGAGCACAACACTTTTAATGTTGGGGTCCTGGGTTCGAGCCCCAGGCGGATCACTGAAACAAAAAGCAAAACAATGAAAACCGCTGATAATCAATCATTATCAGCGGTTTTTCTTTTTATCCATACTGCAAATTGAAGCAGAATACCGCATTTTACTGGAGGTGAAATAGGTGGACTTAATTTCCACATAAAAACAAGTCCACCTGATTGGATTATATTTCACTGATTCTCTGCGTTTTGCATAAAACAAACTCTTTTCAAAACATGTATTTTTACACCATCAAAAAAAGAAGAGTATGGCAATGCAAAGAAACTATTTTACGGTATTGTTTTTCCTGAAGAAATCAAAGCTGCTTAAAAATGGAGAAGCACCAATCTGTATGCGTATCACAATAAACGGAAAACGTGCAGAGGTACAAATCAAGCGAAGTATAGATGTTACAAAATGGAATACGCAAAAAGAATGCGCGATTGGCAGGGAAAAGAAGTATCAAGAAATAAACCACTATCTTGATACGATAAGAACTAAAATCCTTCAAATTCACCGTGAACTTGAGCAGGACGGTAAACCTATTACAGCAGATATTATAAAAAATATCTATTATGGAGAACACTCTACTCCCAAAATGCTGCTTGAAGTATTCCAGGAACACAATTCGGAATATCGGGAATTAATGAACAAGGAATATGCCGAAGGTACTGTACTTCGATACGAACGTACAGCAAGATATTTGAAGGAGTTTATCAGTGAACAATATAAACTGGCTGATATTCCATTAAAATCAATCAACTATGAATTTATAACCAAATTCGAACATTTCATTAAAATACAGAAAAACTGTGCGCAAAATGCGACAGTGAAATATCTGAAAAATTTAAAGAAAATCATCAAAACTGCATTGATAAAGAAGTGGATAACTGATGATCCGTTTGCAGAAATACACTTCAAACAGACCAAGTGTAACCGTGAATTCTTAAACGAAATGGAACTTCGCAAAATCATCAATAAAGATTTTGATATTCAACGATTACAAACCGTAAGGGACATATTCATCTTCTGTTGTTTCACCGGTTTGGCTTTCACAGACGTAAAGAATCTGAAAAAGGAACACCTTGTACAGGCTGATAATGGTGAATGGTGGATAAGAAAAGCAAGGGAAAAGACCGATAATATGTGCGACATTCCATTGTTGGATATACCAAGACTTATTTTAGAGAAATATCAGTCAAATCCAATCTGCAATGAAAAAGGATTATTACTTCCTGTTCCCAGCAACCAACGAATGAACAGTTATTTGAAAGAAATAGCTGATGTATGTGGTATTCAGAAGAATCTTTCCACACATATTGCAAGACATACATTTGCATCACTGGCTATTGCAAATAAGGTTTCCTTGGAATCCATTGCCAAAATGTTAGGACACACGGACATTCGTACAACTCGTATTTATGCCAAAATAATGAATTCTACCATTGCCAATGAAATGAAAGTACTGCAAAACAAGTTCGCAATATAATTTTCAACCATTATTTCATTTCTTACAGCAAATATCGCACTTTGCCACTGACTGTGCAAGGCGGCCCTGTCGGGCTGGTTGGCGGAAAAAAATCATCCTCGCTTCGCTCCGGTATTTTTTTCCGCCAAGCCTTGCACCGGTCATTGGCAAAGAACAGCCGGGCCAGTAAGAAATTGAAATACTGGCTCCACGGAGCCGGTCATGTCTAATTTAAATAAAAGAATATGACTGAAGAAGTTGGAAAGAAGGTATGTGAAGGTACAGTAGCAGACCTCATGAAGGACAAGACCGGAAAACAGACGGTTGTCACGTTGACAAGAAAGAATGCTTACCGAGTGAAGAAAATCAGAGAACAAGGGACGGATGACGAAGCTGTCCTTTTTCATTTCCGTGAACGCTGTACGGGAATGGGCTCCTATGTACACACAATCGAAGCGGCAGACGGAGAAACAGAACTTCATCCGTCTGAATTTGAAAAATGGGAAGCTGTGGAATTCCTGTATCCCGGCTATCTGGAAGACCTGCTTGATGCTGCATACAACGCATACAGATGGAGTTCCTTCGAACCTGAAGCAAGGGCGGAAACAGACATCATGCAATATGAAAAACAACTTGTAGAGGATCTGAAACAGATTCCGGAAGAAAAACAGAACGAGTATACCAGTGCATACCATAGCAAGTTCTCTGCCTTGCTGGGCTGTCTCTCACGATGTGCCAGTCCGATGGTGACAGGGCCTGCCAAATTCAACTGCCAGCGCAACAACAAAGCCTTGGATGCATACCAGAACAGATTTGATGAATTTCATGATTGGCGTAACCGCTTCAAGGCTGCCATGGAAAGGATGAAAGAGGCTGCCAAACCGGAAGAACAGAAGCAAGAGGAGGCATGGAACCGCCTGAAGCGTGACATTGCAAGCAGCGCACAGACCATTCATGATATTGATACCGGTAAAGCAAGAGGATACAGCCGTGCCTTGTTTGTCAGCAGTATCCTTAATAAAGTAAGCACCTATGCAGGAAAAGGAGAAGTGGAAATCGTACAGAAAGCGGTGGACTTCATTACAGACTTCAATGCACAATGCAAAAAACCGGTTATCACTCCGCGGAACCGTTTCTTCCAACTGCCGGAAATGGCACGCCAGGCCAGACTGAAACTTCAGGAAATCAGAGAACGGGAAAACCGTGAACTGAAATTTGAAGGCGGAACGCTGGTATGGAACTATGAGGCAGACCGCCTGCAAATCCAGTTTGACAATATTCCGGATGACCAGAGGCGCAAGGAACTGAAATCATACGGTTTCAAATGGTCGCCGAGATACCAGGCATGGCAACGGCAACTTACACAGAATGCCGTATATGCAGTCAAAAGAGTGTTGAACCTTCAAAACCTATAAGACATGAAAGACCGATTGAAATATGTAATCGATTCCCGCTACTTCGACGGAACATGCCTGACAAGTATGAGTGACGGATTCCATAATGACTATGGTGGGGAAACAATCGAAGAACTGCGCATACGGGAAAACAATCCCTATCTGAAAGCAGTAACACCTTCTGATATAGACAAGAAGCTGCGGCTATACAATCAGTCCCTGTCCGAACCGTTCAAGGAAATCACTGAAGAAGAATACTATGACCTGCTGGATGTACTGCCACCCTTGCGCATGAGACAAAACTCGTTCTTTGTAGGAGAACCGTATTACGGAAATATGTACTCTTTCTGCTTTACTCGTCAAGGAAGATATTTCAAGGGCCTACGCTCCGTACTTACTCCGCAATCCGAACTGGACAGTCAGATAGACCGTCACATGGAAATCATCAACCGGAAAGCCGTGATCTCAAAAGAGGAAACAAGTAAAACGGTCACAACCGGAACCAGACTCATTCCCTATTATTTTTCACTGGACGGAAAACAGCCCGTATTCATCTGCAACCTTGTCATCCAATCAGATTCCAGTCAAGCAAGGACGGACATGGCGAATACCCTGAAAAGTCTTCGCCGGAACCATTATCAGTTCTATAAAGGAAAAGGGCATTACGAAACTCCGGACGAACTGATAGACCATGTATCAGGAAAGAAGCTCACCCTTGTTTCCGACGGACATTTCTTTCAATATCCTCCCGGCAGGGAATCCGCAACTTTCATCGGACACATCAAGGAGACATCAGAGGAATTTCTTTTCCGGATCTATGACCGTGAATATTTCCTGTATCTTCTTAAAAGACTGAGGACCGTGAAAAAGGAATCGGCACAGGAACAAATAAATATCAAATCATAACATTCGGGGGAATGCGGTAAAATGACTGCCGTATTCCCTCATAAAAACAATACAAGTATGAACAAATCAAACACTCTATACTGGAAAACAGCCACAGATCCGGCTGAACGCATTGAGGTCAGACTCGTCCTGAACAGTTATATCGACAATGACAATCTGTATGTAGGACTTGAATCCCGGTCTAAGGAGAATCCGGAATGCTGGGAATCCTACACGGACATCACCGTCAACCTCAATTCTCTTCCCCCGTTCCATGCCTATGTGGACAACCGGGACTGCAACAGACATGTGCATGATTTTCTGACCAGTAACAGAATAGCAGAACCTGCCGGATTTGAATATCAGGGATTCAGAATGTTCCGCTTCAATCCTGACAGGTTGAAGGAACTCGCACCCGAACAGTTCAAGACAATCAGCGCCAAACTGCCACCACAGGATGACATGATAAAGGACATCATCTATCAGGAAAGACGTTTCCCTTTGAGAACTGTTCAAGACATTCACGGAATATATCTTGTTTCAAGCAAGGAACTGGAAGAATCTCTGATCGAAGGAGTACGGAACCTGGATGCTGCGGCATATGAACTGCTGGATGGCATCTGCCTGTTCTGCTCCACACAGGAACTGCGCTATCTTACGGATGCAGAACTGATAGAAACAATCTACGCACAATAAAAAGGAGGAACAAATATGAAAACCGGAGACATTGTATTTCTGAGACGTCCCTATAAGGGATACCGTGCCGTCGAACTGATGGAAAGACTGGAATGCCGCTGGCTGGTCAGGATTGTCGAGAGCGGTCTTGAACTGGAGGTATATGAAGATGAACTTATATCAGAATTTTAATACAGACAAAGTGTTATGGAAAAATATCAGTTTGCATTCCATTCGGAAATAATCGGCTATACCTCTCCTCATATCGGTGAGGTCAGAAAAGCCATACACAGAAAAGTGGAAAAGGAAAAGTCTGCCGCCATAAAGAATGATATTGAGCTGCACATGTACAAAGTGCATGACGGCATACCGGTTCTCCTTAACACCTGCTACCTGTACGATGAAAAAGGATGTATGGTACACGGAAGTATCAAGGGAACCAAGGATTATCTGCTTGAGACATGGAGATACCATACAAACAGACATTCTAAAGGCATCAGTTCCACAAGAATCAGGCCTTGCACGACAAGCAGGGCTTTTTCATTTGTATAACTCTTAAAATCAGAAATCATGAACCAGACATTACAACTTACAGACTATATTCCACAGAATGTAAGCCTCTACTACGTGGACTACCGGGATGATCTTGATGAGCATGAAGACATCCAGGAGGAATGCATCCGTTCCAACAAAATGGAAAAACTCTATGAAAAGGCATACGAATGGTATGAGGAACAGGAAAGTTCAAACATGCACGACTATCTGGAGGAGACAAGAAAGAATATGGAAACGGACAATTTAGCCGGAGAGTTTGAAGAGCATGAAGATGAAATCAGGGAACTTATCTACGACCGGAACGATTCCGACCCGGTAAAGGATATGATACGCAACTCGTCCGTCACTAATTTCTTCTATTCGCTCGGAGTGGAAATCAGCGGATATCTGACCGGTTGTTCACTGCGGGGAGAATCAGTCGCCATGGCCTGCCATAAGGTACGTCGCGCACTGCATCTGAAAAAGGGGCAGTTTGACGAGAAGATTGAAGAACTGGTAGAGAATGCCACATACGGTGGAGAACTGCGCATCTACTTCAACGCCATGTTTGACAGGCTCATCAGCAAAGGCCCTGAGAACGATTTCAAGAGCATCCGTTTCCACGGGAATGTAGTGGTGGTCATTGCCGACAGCCGGAACGGTTCCGGACATCATGTACGGATTCCGCTGGACATCACTTTCCCTTTCCGAAGGGAGAACCTGTTTGTCGATTCACAGGTACACTATTCCTATGCCAATGAAGTCTGCGGCATGACCAATGACTGGTGTGATTCCACAAAATGGGAAACAGGCATGATACCTTTTACCGGATCTGTCCGAAAAAGCCGGATGGCTGAATACAAGAAACAGGAAGCCGCTTATGAGCAGACATTCCGAGACGGGAAATGCACCTTCGGTGACATGAACTACAAACGCCACCGTGACGTGCGGTATTCGAATGAATATCCTGCCGGATGCAGGTGCCCTCATTGCGGTACATTCTGGATTGACTGAAAAAACATTTACCAACCAATAAATTCAAACGATATGAAAATCTGCTGTTCACAAGAGCATTACGACAAGGTCGTACAGTATGCAAAATCAATCAATGACAAGACACTGGAAAACTGTCTTGAACGTCTAAAACAATGGGAGAAGAACGAGAACCGTCCATGCGAAATCGAACTCTATTACGATCATGCGCCGTATTCGTTCGGATTCTGCGAACGTTATCCGGACGGAAATACAGGCATTGTCGGAGGACTGCTGTATCATGGAAATCCGGACGAATCCTTTGCCGTCACCATGGAACGTTTCCACGGATGGAGCATACATACCTGACATATATGCGACAGTCTGTATTGGGGAGCCTCATGCAATATGGGGTTCCCTTTTTTTATGCCGCAGACATGATGACAGCATCCTCATTTCTTGCTGCAAAAATAGCTGTTTGCCGCGCAACTCCCGCAAGGCGGCCCTGCCGGGCTGGTTGTCTGGAAAAAAATCATCCTCGCTTCGCTCCGGTATTTTTTTCCGCCAAGCCTTGCAGGGATGCGGGCAAACAGACAACAGGGACAACAAGAAATAAGAATGCCTGTACCTTACAGGCAGACAATGTATAACAATAAATATCAGAAGTCATGATTACAGACCAGAAGACACAGAACAGGCTTCACGCGGATACCGGAACGGAACTGTTCTCCATCAGACAAAGGAAGGAAGCCGTCACAAGGATGCTGGACATTCTGAAAGAGACTCCGGAATACCTGCAGGTTATGAACCATATACCGGCTTATGCCATGGATGACGATACGTCAGAATGGTGGAAATCGGAAGAATCGGAAAATTTCATGAACTCACTCCTGGAAGTGATGGAAAGCTATACTCCGGACGGATACAGGTTCGGACCGAAATCCGGCACGACTGACCTTTACGGCTACTGGGAAAGCAAGACCGGGCGGACAACCCTCTTCCATCTGCTTTTCAGTCTGGAAAGCGGATATGAATGGGGAAAAGGTCTTTCCCATGAGAAAACGGACGCATTCTACAAGGAAATAAAAGAGAAATTTCATGGAGAAGGATTCGACACGGACAGAACCGGCTGTACATCACAGGCCATGTATCTTGTAAAAGGAAAAACACGCCTGTACGTGCATCCGATGGAAATAAGCGGCTACTGTGAAACACTGCATATTCCACAGATTACAGCCATACTGAAAAAAGGAGGCCGTACATTCCGTCTTGTAAAGGATACGATAGCGGAAGAGGTGTATTCCTTCACCGATGAAGAAGAACTGGAATATTACCGTGCCAGATACGGAACGTGCATCCACCGGAATATACTGGATGCCTTCAGCAACCGCCACGCAGGGAAAGAGGACATACTTTCCATGATGGCATCACGGATAAATGTGGCTACGACATCACATCTTTACGGTATCGGATATGATTCGCCTGCATACAGGTTTGTGCATGAGGCATACGACAGACTGGTAAACAATGGAAAGCTGAAGGAGAATGTCCGGGAAATCGGTTGCTGCAACATCATAATGGCCATTTCAAATACCAACGCAATATGAGACTGAATTACAATGACATGCTGCTTCTGGCAATATGGGAATACAACAGGAGACAGGACGAGGATCTGACCCTGGAACTGTTTCAGGAAACATTCGGACAGGTTCCCGGCGCACATTTCCATGACAAATGGGTGCATTATTACAACAAGAACCTGCTGATGATGGCCGCCTATTTCAGGGGTGAGGAAGAAAACGGCCAGAAATTCTGTGATATGATCACCCGACAGGTTGAACGCTATACACAAAACAGGAGGAGAACAGGATGAATACAAAGATACGATATGACCTTGACAGTCTTGAACTGGCAAACGGTGACTTCGGGTATCCCATTACAGAAAAGGAAGTACGGAAAGTGAACCGTATGCTGGAACTGATGGAGAATGTCCGAAGCAGGCAGATGTGCCCGACAGAAGGAGACTGCGTGGAATTTGTCTCACGTTCTGGTGACTATTTCGGAAAAGCTCATATAGAACGGATAACAGGAAAATATGCGGATATATGCCTGATACCGGAAACGGTATTCTGTTTTGATGACATGGGAAAAGCCGCCTATGATACCACCGGAAGTCCCTGGACGCAGGTCAATATCCGGAACATGAAACCCGCAGGTTCTGAAATCCGCATATTCAGAACATGGGGATTCGGGAAGCGCAGCAATACGGGCAGTCTCAGGTTCGATGCTCCGGTCAGGAAATGGGAATACAGAGAACCGAATCCGTTATATGACGGTTACACCACCCGTAACTGGTTCCGCTATCATATCATGAAACACCGGGACAGGGAAAGGACAGGCGAATACACCTTCCGCAGCGATTCATTCACGCTGTACAGCCGGAGCGAGCTGGACGAGCTGGCCGCAATCCTGAAAGGCAGACTCTACAAGGGAATCCTGCCTGACTCTCTTGTACTTTGGGGATACCGCATGGATATTAAGGAAATATCACGTGAACAGTGGAACGGTATGGGACAGCACGGACAAATCCGCATGAAATTCATGGGATACGGTCCGGTCAGAATCCACACGGACAATGAAAACCATACCGTAACAGTATACAGAATCAACGACATATTGTCTTCAACTATCAGAATTTTCATATTTTTTCAGTTCTTTTTTTGTTTCTTCTATTAATATTTTAAGCCACTCCATGATTTGTATTGCATGTTCATGAACAGTTTCATTTTGGCTATCACTGTCGTGTAGTAGCCTTTGAAAATCACGTAAAATATTGTCTTTCCCAAGCATCTCCCATACAGGCATCATCCGGTGGATTATTTTTCTCATGGTCTCACGGTCGGTTATCCTGTCAGCAGATTCCATCTCCTCCAGTTCTTTTTCAGATTCCATAACAACGAGAGAAAGCATATGACTATAATCATCCGTATTCTCCAGTAAACTGGAAAAATCGAATTCTCCGGAAACTGAAACTTGTGTACGAGATATAATGGTGGATAAAAAAGCAAGCAGTCCGTGGATATTGAACGGTTTATGAATACAGCCTACAAATCCTTCTTTTTCATAAATTCCGGAATTTCCGTCACCACGGGCAGTCATGACTGCTACTGGAACAGTTCTAGAATTGCCGATGTCCGAATTGCGAAGCAATCTTAACAAACCGAATCCGTCAGTATCAGGCATTTGTACATCTGTCAAGATCAAATCATATTCAGAATTTTCAAGAGCGGCCACTACTTCACGTGCATTCTTACAGGTTTTACAGGATATACCTTTGCGCCCGAGCATATCTTCCGCTATTTTCAGTTGTATAGGATCATCGTCCACTACAAGAACATTCTTAGGCAATATAGTTATTGTATTATGGTCCGATTTGTCTTCCTCAACTAACTCATCCGTTTCAGGCAAAGAAAGTTCCAGTCTGAACATGCTTCCTTTACCGAGTACACTTTCTACATCCATTTTTCCTTCCAAAACCTTAATTAATCCTTTGGTAAGGAAAAGTCCCAAACCAAACCCTTCAGAATTGACATTCTGTGCGGCACGCTCAAATGGAGCAAATATTCTTTTCAGTGTTTCCTCATCCATACCGATACCAGTATCCCTTATTTCAATACGAAGTTTTCCTTCTGAATATTCTGAATGGAAATTGACGTTACCCCTGGAAGTAAACTTAATAGCGTTTGTAAGTAGATTGGCTAAAACCTGTTCAAGTTTGTCCGCATCACCTTTTACTATTACATTTGATCCTTTATGTTCAGAATATAAAATCAGACCTTTTGAAGTCGCTTTACGAGAAAACTCATCTGAAATTCGTTGCAAGAAACGGTCAAGATAAAATGGTGTGTCGTTACGCAAATTACCGGCTTCATTGATTCGGTAAGCATCCATCAAATCATTAACCAGATGTAAAACGTGTCGACAAGAATGACGGATGTCATCTAAATATTTTTCGCGCTTCCTCTTTTCACGCGTTTCAGATACCAAATCTGCACAGTTATGGATATTACCAAGTGGACCTCTAATATCATGAGAAACTGTCAGGATGATTTTCTTACGCATATCAAGCAAATTCTCGTTTTCTTGAATAGCTTGTTGTAATTTAAATTTAATTATTTCTTCCTTACGTAAATCTGATTGTATAATTAAAAATGAAATTAATATTATAAAAACCGCAATACTCATCATTACGATAAATAATCGAAAGGATTCTTGTTTGACTTCCGTTACCTCTAAGTTTCGTTCTATAAATGACAGCTGTACCTGATTATCTAAAAAAGATACAAAATCATATAATTTTTGATTTAACAGCCTATTCTGCAAACGCAAGCTATCCACATAAGTTTCTATCTGATTGTTTCGCATATCTATGACAGAAACCAATCTATTATTAAAATTCTGTATTTCATTAGTTATATAGGGGACTTGTATCGTCTCCTTCTTTCCGAATAATCCGGCAATTCCTTTCTTTTTCTGAGTTATTGTCTTCACTTTTACTGTTTGAGTAGCTATTACAGGCAATTCATTAGTAAGAATACTATCAGATTTATTCGCAAATTGGACTGCTTTCATTATTTGAAACAAGTGCATTTCTTTCGTTTTAAGCAATTCCCGTAAAGAATCAATTTGAACTGGACATAAAAAATCACAACTCCTTAATTTTATTTCAAGTAGAACACTATCTGTTTTAAAACGTTGATTATGAAATATGTTATAATCAGACTCATCCCATACTATAACTGATTCGCCTAAAGTTGCCAACTTAGTAATATACAAATGAACTTTATTAGTATTCTCATAAGCTTCATTAATTTGAATTATCAGATTCTCAAGTTCTTTCAACCGGCAACGTTCATTTATCATTACAGTAACCATACTTAAGACTATAAATCCTGTAATAAAATATCCAATAAATAGTCTTTTGCGTAATAATGAAGTCATCAGGAACATTCTATTGATTTATTTGACATCATAATTCTATATATTTAACTAGTCATAGTATATATCATTCTCAAATATTTATTTCAAATTCAAGCAATAAAATAAAAAAACACTTCATATTACAACTGAACTCTTTTATGAAAAAGTTGAATATATGAAGTGTTTTTTTATTACGATATAAACTATAAAATCCTATTCTTCGGGAACTGGTGTATAAACCCTTATCCAGTCCACCAGGAAGGTGTGGTCTTCCACATTTTTCAGTTCCTCATCCGTAGGGCTTAAACCTTTAACGGCTCTCCAGCTTTGGTCTTCCATATTTATTATGATGTCCATGTCTTTTACCAGACCTGTACCACCAGTGTAGTTGTTGGGGTCGATAATATCCTTGCCGCTTACGGTTCTGACAAGTTCTCCATCTACATAATATTCAAGTGTGAAAGGGTCTTTCCAGAACACTCCTACACGATGAAAATCGTCGCGCCACAATGTTCCCTTGTCATCCTTATACCATGAGCCAAGATCTTTCGGCTGATAATCCTTGAATGGCTGGCGGATGAATATGTGATGGCTCAGGTGAAGTCTGTCGGCACCGTAACCTCCGCCGTCTCTGTCGCCGCCGTATGCTTCTATGATGTCGATTTCCTGAGTATCGTCAGGGCTGAGCATCCATACATCGGATGCCATGGTTGAATTTGAAAGTTTTGCGTATGCCTCTACATAAACCGGATACTTTACACGTGTCTTCGATGTGATACATCCCGTATAGGTTCCCGGCAGTTCCTTTGTGTTGGGTCCGCTTACAACTTTCTTCATGGGGACATCTTCAGGACGGCTGGCTCTTATTTTAAGGTATCCGTCGGAAACGGAAACATGGTCTCTCTGCCATATTGTAGGAGCAGGTCCTGTCCAATGATTATGATAGAAATCGGTCCATTTGGCATAGAACTCTTTTCCTTTATCCTTTTCGTCGGCAACATAATTAAAGTCGTCCGACTGTGGATGGAGTTTCCACACCATACCGTCGCCGGCATCAGCGGGTACAGGATAGATATCCCACTCGTACGATTTATTATTGAAATCTTCTGCTGCACAGGCTATTTGCAGCGATGCTAAACAAATGGTAAACAGTTTTCTCATCGTGGTATCTTAGTTTAAGTTATAATAATTATTTTCGTTCTTTTGATTCACCTTTAGCGGTATGTGTCTGCAATGTCCAGGTAGAAAATCTCATTATGCTCTGATAGTCTGAACTGTTGTATATATGAGTAAGACCCCATCTCAATATTTCGGTAGGTTCTTTTTCGGCATCTGCACTGCGGTTCAGGCCAATGGCGTGTGGCGCGCCTTTTACTACTGACATTATTTCAAAGTTTATTCCGTCAGGCGACCACTGGAGTGTGTTCTTTTCAGGACCGTCGGTGGTGATAAGTGAAGCTATACCTCCTTTGTAAGGCCATACGCAAACTTCATGCCCGCTGTTTGAAATAGGATTATATTCCGATTTCACATACGGACCCATAGGATTTTCCGCAATAGCCACTCCGTGTTTGATTTCACGGCCGCCCCATGTTATTTCTTCTCCCATACGTTCGCCTTTGTAGTACATATAGAACTTACCTTTATAAGGTATTATACACGGGTCGTGTACCTTATGACTGTCGAAATCACCTTTCGACACTACCTTGAATCTGTTATCCTCATCGCCTTCCCATTCGCCGGTATTAGAAGGTTCCAGTACAGGCTTGTCTGTCTTGATCCACGGTCCTTCAGGGGAATCAGCACATGCCATACCGATAGTATTCTTTACACGGACTGTGTAAGGGGATTTTACCGCCTGATAGCAAAGATAATACTTTCCTTTCCATTCCATCACCTCAGGAGTGAAGACTGAACGGTCGTCGTAAGCACCTTTTTCACCACGTTTCACTGCAATTCCCTGTTCCTTCCATGTCCATCCGTCTTTTGATGTGGCATACCATATATCACATCTGTCCCATGGGAAAACCTTATCTTTCTCTATATCTCCAGCAAATCCTTGGGTAGGTCCATAGCTCTTTGAATACCATACATAATATGTATTACCTATTTTCAGCATTGCACTCGGGTCTCTTCTTACTACGCCCTCTTCATAAGCAAGATCACCTTTAAGTGGTTCCATCTTATACTCAAAGAACCATTTATTGTCGTGATTTTCCCATTTCATGGCACGTTTCATAGCTGCACTTAACTTATTTCCCTTAGGTATTCCCAATGAATCGGCCTTACGCTCATCATAATTCTGAGTGTCGTCAACGGCAATAGTCTGTGTATTGCCTGTATTTCCGCATGCTGCCAATAGCGACATCATGCCGGCTGCAAGAATAATTTTTCTCATACTAGACTTTATTTTATATTAATTGTTAGTTTATTCGAGTGTAATTCACTTGTTTCTGCACTGATATTCAGTACCGATGATTTTTCTGTCGACTGAAGCATCAGCATACATCTTCCCTGATATGTCATAATATCCTTACTTTGATATGGAGAAACGTTCTTCACGTTTCCATTGTCTATACCAAGCAGACGGTACTCTCCATCAATGTTGAACTTAAGCATCTGTTCTGTTGTCTTTACAGGATTACCTTTTTTGTCTGTTAGCTGAGCTGTGACATGCAGAACATCCTTTCCATTTGCTGCGATACTTTGTTTGTCAACCGTCAGCAATATCGAATGTTCTTTGCCTGAAGTCCTTATAGCTGTAGTGGTATTACCTAACTTATTTTTTCCTTTTGCGGTAATAGTGCCAGGCTTGTACTGAACTGCCCATTTATAGATATGATCCTCAAAATCGTCTATATACTTCTTTCCCATCGACTTACCGTTAACGAAAAGTTCCACTTCATCACAATTGGAATATATCTCTACTATTACCGAGTCACCTTTCTGATAATTCCAGTGAGAGTTTACATCATCCCAAACCCATAATTTTCTATCCCATTCATGTCCTTTCTTATCAGTAAATCCATCTTTTACATGGAGATACGAAGATTTGTCTGTAGTCTGTGAATATATAGCAATAAAAGGCTTGTCTGTCCACAATGATTTCATCATGTCGTACGAAGGCTTCACATAGCCGCACATATCCAGGAGACCACATCCTATCGACTTTTGAGGCCATTTTGAAAGACGGCTTTCACTTTCTCCCAGATAATCGACTCCTGTCCATATAAACATACCCGGAACGAAATCCCTTTCAATCACCGCCTTCCATTCGTGCCACTGACCGAGATTTTCTGTACCCATTATAGGCTTGTCAGGATAATTCTTCTTAGCATAATCATACATCACGCGACGGTAGCTGAAGCCTGCCACATCGAGCGCGTCGATATATCCTGACTCAAAGCTTATGGAAGGCAGGATGCAGTTGGCGGTAACTACACGTGTGGTGTCCATCTGGCGTGTCCATGCAGCTAATTTTTGCGCTGTACGGCCAATGTCGTATGCATGTTTAGGCTGGATTTTCCACATTTCTCTGATTTTTTCTTTAGAGTATGGAGGCTGATTCCAGAAATAATTACCGTTGGAATCGGCACCGAAGAAACCTGTCGCCTCGCGGCATCCGGTATAAGTCCATTCTATTTCATTACCTATACTCCACTGGAAGATACAGGCATGATTACGGCTTCTCCTCATTACGTTTTTCAAATCTCTTTCTGCCCATTCCTGGAAATGCTCGCAATAGCCATGCGTAGGATAGTCTTCTACAGTTTCCTTCATATTGAGTCTTTTATCTTTGGGATAATCCCACTCATCGAAGAATTCTTCCTGAACCAGAAGACCTATCTCATCGCACAAAGACAGAAACTCTTCCGCTCCCGGATTGTGCGAGAGGCGGATGGCATTGCATCCTCCTTCCTTTAGGGTTTTCAGACGCCGGTACCACACATCGCGTATCATTGCCGCGCCAACCATTCCGGCATCATGGTGCAGGCATACTCCTTTTATCTTCATGTTTTTCCCGTTAAGGAAGAAACCTTTGTCTGCATCAAAACGGAATGTCCGTATGCCGAACCTGACAGTGTTTTCAGAAATTACTTCATCGCCATTCTTGATGCGTGTCTCGGCTGTATAGAGGACAGGTGTATCGACGCTCCACAAATCAGGCTGTTTAATCTCAGATACGATGTCGATAATTTTCTCCTCACCAGCATTCAGTTTTATACTGAAGACCTCAAAGGCTGCGATATTGCCTTTATTATCCTTATATACTACCTCAACAACTGCAGCTCTGGGTTCGGAGTAGCTGTTGCACACGGTAACCTGGTTGTTTACTTTAGCATATTTATCAGTAACCACGGGAGTAGTGACAAATGTTCCCCAAACCGGAATATGCAGTCTGTCGGTTACAATCATTTTCACATCCCTGTATATACCTGAACCGGTGTACCATCTGCTGTCGGCATAATGGCTGTGGTCGACCCTTACAGTCATACGGTTATCCTCATTGGGATTGAGATAGTCTGTGACATCAAAATAAAAAGGAGCATATCCCGAAGGATGATATCCAAGCTTTTTGCCATTTATCCAATACTCAGAATTATTATATACTCCATCGAACACTATATAGCATTTCTGATTTGCACTGATTGTTGTGGGAAATGATTTGCTATACCATCCTATTCCTCCCTGAAGGAAAGCTACACATCCTTCACCCGAAATGGAATCGTAAGGTAAACCAACACTCCAGTCATGTGGCAGGTTCACTTTCTTCCATTCATCACCAGGGACATAAGAAGTATATGAATAATGAGCAGAATCTTTCAGTACGAATTTCCAATCTTTATTGAAATCAACATTTGAATCAGATGCTGAAACCTTTAGGGTTGATAATAGGATTATTAAAGCTAAAAGATTTTTATTTCTCATAATCTTAGGTTTTACATGTTTTTTGATGTCACAAAACTATATCTTTCACTTATAATATATGAGGGGGATATTAATGTGATATAGGGTGGGAAATCAGAATTTTACATCTGCCCTGTATTCCACCGTCACCTACAACCTTGACAAAGGATGTTCCTTTCTTCCCTCTTATGGTTCTCAGGACAAACAGACACTTTCCGTTATATGTCCTTACACTATTGTTTATGACGTTGATGTTCAAATCTTCTATCGAAGGCGATCCATTGTCGAGTCCGGCAAGTTCAAGCTTGTCGTCGAGGATTATCCTCACATCCGAAGGTATATCGACTACTGTGTTTCCTTCTTTATCTTCAATGGATACTTCTACATGGATAAGGTCATAACCGTTGTCGGTAGCTGTTTTGCGGTCGCAGTTCAGTGCCAGACGGCACGGCTTGCCGCTTGTGGACAAAGTGTCTTTCGACAATATTCTGTCGCCGTCCTTGCCTACCGCAAGGAGTGTTCCTTCCTTGTATGCCACCTTCCACATCAGTATATTATGCTCCATGAAATCGCTGCGTTTCTTTGTTCCCAACGATTTGCCGTTCAGAAACAGTTCCACTTCTGGGGCGTTGGTATATACCTGCACCAGTATGTCCTCGTCCCTGCGGTACTTCCATTTATCGCGTGTGTCGTACCACTCCCAGCGTCTGATCCATCCCGGGCGTGGAGTGTAGGTGAAACTTCCGTCAGTATCCATCTTGAACTCGCTTTCCTTTTCAGGTATTGTTACAATATGGGTTTTCGGTGTGTCTTTCCACAGACATTCAAAGAAATGGCCACGCGCTGTCTTGTTGCCCACGAAATCGAAGAAAGAACAGTCTCCACCCCTTGCAGGCCATGGGCCGTTCTCGCCAAGATAGTCGAATCCTGTCCACACGAAGATGCCCGCTATGTACTTCTTGTCGGCCACGGCTGTCCATTCAAAGAGCTGACCAACATTCTCCGAACCGATAATAGGCTGATATGGATATAGCTTATGGTCGATTTCATAATATTTGTCTTTATAGTTATATCCCACTACATCAAGAACGTCTGTATATCCGGAGAGACGCGAAACTGACGGAACAACGACTCCTGAAGAGACGGGACGGGTAGTGTCCACATCCTTAACCCAACCGGCAAGGACAGCGGCTGTTTCAGCCAAATCGTCTTTTCCTCCTGACAGACGGTTGAACTCTTTCAGTATAGACTTGTTGTCTGTTTCCGGGTCGCCCGTATGGATAAGACCCTTGAACCCTTTATTGTCTTTGCTCGATGCCCAGTAATATGGATAGGTCCATTCTATTTCATTGCCTATACTCCAGAGTATCACGCAAGGATGATTTCTGTCTCGCCTGATGAACGACTTGAGGTCGTGCTCGGCATGCGTATCGAAGTATCTGGTATATCCTATTGATATGCTGTCGGGCGCATCTTCCTTAGCTCGCTCAGTAATCCACTTTTTCTTTGCCACCTTCCATTCGTCGATAAATTCATTCATTACAAGAAGTCCCAGACTGTCGCACATTTCCAGCAGACTTTCCGAATGCGGATTATGGGCTGTACGTATGGCATTGCAGCCTATGGAACGAAGTTTCAGAAGGCGTCGCAACAGGGCATCATCGTATGCGGCAACACCCATACATCCCAAGTCGTGGTGTATGTTCACTCCTTTTATTTTTACTGATTTTCCGTTTAGAAGGAAGCCTTCATCCGCATCGAATTTAATGTCGCGGATACCAAATTTTGTTGTTTTCTTATCCATCACATATCCGTCAGAAGCAATCAGAGTAGTATGAAGCTCATACATCGAAGGCGTTTCAAGACTCCAGAGATGACAATTCTCCAGTTCAACAGATGCAGTGAACTCATTGAAATCGCCTTTCAGGGCAACAAAATCATCGGAAACAGAAGCTATTGTCTTGCCGTCGTACACTACTTCGTGCTTCACGGTGACTCCTTTTACACCTGTTCCAGCATTCTTCACCTCGCATACCACATTCACCATCGAACGGTTGCCTACCTGTGGTGTGGTAACGAATATTCCGTCTGAAGGAATATAGAGCTCGTTTCTTAGAATAAGACTCACATTCCTGTATATACCGGCACCGACATACCATCTGCTATCGGCATACGCTCTTCTGTCAACGCAGACAGTTATTGTATTCATCGAACCTTTTGGTTTCAGATATTGAGTAAGTTCATATTCAAATCCCACATATCCGTTAGGACGGAATCCCAACATATGCCCGTTTATCCAAACCTTTGAGTTATTATATACACCTTCGAAATGAATGAACACTTTTTTCCCATTCATATCATCCGAGGTGAGAAAATTCTTCATGTAAATCCCCACACCGCCAGACAGAAAACCATTGCTTCCGGCTGTCTGAGTCTTGGTATATCCTTCGCTGATACTCCAGTCATGAGGCAGACACACATCCTCCCACTTTATATCTGGACTCAGGAACAAAGTGTCCTGAGGCACGAAACCTGCTGGTTTGCTGAATTTCCAATCGAAGTTGAAATCCACTTTAGTGGAGGTTCCGGCATAACAGAATCCGGACAGAAAGATAGTTAAGACTGTGATAATGTTTTTTATGGTCATATCGATTTTCAGATTAATATTAATGACAAAAATAATTTCAAAAGTGTAAAAACAAAAAAACTCTCCATTTATATTTCAGATATCAACGGAGAGTTTCATCATTAAAAAAAATAAAACATTTTATAAAGTTACTCCTTGCTTAAGGATAGCTATTTCCCGGTATCCCTTCTTTTCGTTCAGTGCCTGCTTTCCGCTTGCCACTTCCACCACAAAGTCTATAAAACGTCTGCTTAAAGATTCCATGCTTTCTCCCTCTACCAGAGTTCCGGCATTGAAATCAATCCACGTATGTTTCTGTTCATAAAGCGGAGTGTTGGTCGAAACCTTCACGGTTGGAACGAATGTTCCGAACGGTGTTCCGCGGCCTGTTGTGAACAGCACGATATGGCATCCGGCAGAAGCAAGAGCCGTACTTGCCACTAGGTCGTTGCCTGGTGCGCTCAACAGGTTAAGTCCGTGTGTTGTGACACGGTCGCCATATTTCAGAACATCCTCCACCATCGAGCTTCCCGACTTCTGTGTACATCCCAATGATTTCTCCTCAAGCGTGGAAATACCTCCCGCCTTGTTTCCCGGTGAAGGATTTTCATATATTGGCTGGTCGTTGCGGATGAAGTAGTTCTTGAAGTCGTTTATCATGGCCACTGTGTCGTCGAATATCTCCTTCGTGCGGCAACGGTTCATGAGCAGTGTCTCGGCTCCGAACATTTCAGGTACCTCCGTGAGGACTGTTGTCCCACCCTGGGCAACAAGATAGTCAGAGAACACCCCAAGCATCGGATTGGCCGTGATACCGGACAGTCCATCAGACCCGCCGCACTTGAGTCCTATACGCAGTTTTGACAGGGGGACATCAGTCCGCTTGTCTTCCCTGGCTATGGCATACATCTCACGGAGAAGTTTCATACCCTCTTCTATCTCATCATCTACTTTCTGAGAAACAAGGAAACGGATCCTTTGGGTATCATAGTCACCTATAAACTCACGAAAGGCATCAGGCTGGTTGTTCTCACAGCCAAGACCTACGACAAGGACAGCTCCGGCATTGGGATGAAGGACCATGTCACGCAATATCTTACGGGTGTTCTCATGGTCGTCACCCAACTGCGAGCATCCGTAGTTATGAGGGAAAGATATAATGGAGTCAACCCCCTCGCAACCTGTTTCCTTGCGAAGCTGCTCGGCCAACTGGTTTACTATTCCGTTCACGCAACCCACCGTAGGGATAATCCATATCTCATTACGTATGCCGGCTTCTCCGTTAGCACGCAAATACCCTTTGAATGTATGGTTCTCGTTCGTGAATGTCTGTTTCTCGAACTTCGGAGTGTAAGTGTATGTACTCAGACCGGAAAGGTTCGTCTTGACGGTTTTCTCGTTCAGCAGATGTCCTTTCCTGACTTCCTTTACAGCGTGCGATATGGGGAAACCGTATTTTATCACCATATCACCTTCTGCAAAATCCTTCAGGGCAATCTTATGACCGGCAGGTATATCCTCCATTAATTCTATGGAATTGCCGTTCACCTCTATTACAGTCCCTTTGGACAATGGGTGCAGTGCCACAGCCACATTGTCCGCAGGGTTTATCTGGATATATTCAGTCATAACAAACTAACATTTATAAATTGAAGAATACAGGTAGAAGTATCAACCTACAAGGTCTTTTACTGTCTGAAGCATTCCTTCGCTCTGGATTTTGTTGATATAGTAAATTACACGGTCTGCCAGTCCCGAGATAGTATTAAGGTCTTCACCCCAAATGGAAGTATCGGCGAGAACTGTCTTCACAAGATTTTCTACCGAGCCATCGTTCCACAAACTTGTAAGCATCGCCATGATTTCCTGTGCATCGTTAGGAACTATCTCTACACCATCGGCACGCTTTCCACCTTTGTAGTATACTATGATGGCTGCAAGACCGAGTACAAGTCCTTCAGGAAGCACACCCTTACGTTTCAGATATTCCTTCACTCCTGGAAGGTCGCGTGTGGCATACTTAGGGAATGAGTTAAGCATGATTGATGTTACCTGATGGTCTACGAAAGGATTATTGAAACGTTCCAGGACATCATCGGCAAACTTCTTGAGTTCCTCTTTCGGCAGGTTGAGGGTCTCCATCAGCTCGTCGAACATCACACGTTTGATGAACTTGCCTATCACCTCATGTTGGCATGCGTCTCTCACGATATTGACGCCCGAAAGGAATGCCACCGGCGACAATACAGTGTGAGGACCGTTCAGCAGAGTAACCTTGCGTTCATGATAAGGCTCCTCCGACGGGACGAACAGAACGTTCAGTCCCGCCTTGTTTGCAGGAAATTCTTCGGCAACCGATTCCGGTGCTTCGATAACCCACAGATGAAAAGCCTCGCCCTGTACAACTAAATTGTCATCAAAGTATAGTTTAGTTTTTATGTTGTCTATGTCTTTACGAGGGAAACCCGGTACGATACGGTCCACCAGTGTGGCATATACACCACATGCAGTTTCAAACCATGACTTGAACTCTTCGCCAAGGTTCCACAATTCAATATACTGATAGATTGTTTCCTTCAGTTTGTGACCGTTGAGGAAGATAAGCTCGCATGGGAAGATGATGAGTCCTTTCGACTTGTCACCGTTGAAATGTTTGAATCTGTGATAAAGCAACTGTGTCAGCTTGCCCGGATAAGAGCTTGCAGGAGCATCCTCAAGCTTGCACGACGGATCGAAGTTGATACCGGCCTCAGTAGTGTTCGAGATTACGAATCTCATATCAGGCTGTTCCGCCAGTGCCATGAAGTCATTATACTGGCTGTATGGATTCAGCGCGCGGCTGATGACATCAATCATTCTGAATGAGTTCACCACCTCGCCATTGTTCAGTCCCTGAAGATTGACATGATACAGACAGTCCTGGGCATTGAGGGCATCAACCATACCTTTTTCTATAGGCTGCACCACAACAACACTGCTGTTGAAATCTGTCTTTTCATTCATATTCGAGATAATCCAGTCGACAAACGCACGAAGGAAATTACCTTCGCCAAACTGTATGATACGTTCCGGACGTACTGCCTTTACTGCAGTCTTACTATTTAAAGCTTTCATTGTAATGCCAAAAAATTAAAATTGATAAGATTAAAATTCAACCAACATTCTGAATACCTTACCTGGATTTTCCGACCATTTCTGCAGAGCCTCGCCTGCCTCTTCAGGTTTCACTACGGCAGAGATAAGTTCGTTCATCGGGCAGTTGCCATTCTGAAGATAATGTATCACGGCACGGAAATCCTCAGGCATTGCATTGCGCGAACCGCGTATGTCGAGTTCCTTCTGGACAAAATATTTTGTCTGGAAAGCCACTTCACTCTTGGCATAGCCGATACATGCCACACGGCCTGTGAAACCTACAATGTCGATGGCAGTAACATATGTGATAGGACTACCCACAGCCTCTATCACCACATCAGCCATATAGCCGTCAGTAAGTTCCCTTACTCTTTCCACCACATTTTCAGTCTTCGAATTGATAACCATCGAAGCACCCAGGCGTTTTGCCAGTTCAAGCTTCTCATCGTCAATATCCAATGCTATTACCCTTGCGCCACGAAGCGATGCTCTTACTATGGCGCCAAGTCCAATCATTCCGCAACCAATCACGGCCACAGTATCAATGTCAGTTACCTGAGCTCTCGACACGGCATGGAAACCTACGCTCATAGGCTCAATCAGCGCACATTCCTTATCCGAAAGACCGGCAGCCGGAATAACCTTTGTCCAAGGGAGGACAAGGAACTCCTGCATAGAACCGTTACGCTGAACACCCAAAGTCTCGTTGTGTTCGCAGGCATTCACACGTCCGTTGCGGCATGAAGCACACTTTCCGCAGTTGGTATATGGATTTACTGTCACGTTCATTCCCTTCTCGAAACCGACAGGAACGCCTTCGCCTATTTCCTCTATCACAGCACCCACTTCATGTCCCGGGATGACAGGCATCTTCACCATAGGATTTCTTCCCAGGTAAGTATTAAGGTCGGAACCACAGAATCCGACATATTTGATACGAAGTAAAATTTCTCCGGCTCCAAGTGTTGGTTTAACTATATCAGCTACTTGAACCTTTCCGGCTTCAGTAATTTGTACAGCTTTCATAATCTATGTATTTATTTAAATTTGTTATTGTATTATTTTGATGTTGCATTAATTCAATGTTGTTTTTTCTCTATCTTATATCCTCTCCAGCCATAATATGCCGTAAAGAAGAAACATATCAGAGGTATTACATATGCCACCTGATAGAAGTCCGCGTTATGATTCATCACAAATGCGGTGAACTGAGGGATGCACGCATTACCTATAATAGCCATCACAAGGAATGCCGAACCACTCTTTGTGTCCTCGCCAAGGTCGCGTAGTGCAAGTGAGAACTGGGTTGGATACATTATCGACATGAAGAACGACACTGCAAGCATGGCATAAAGTCCTGTCATACCACCGAACATGATAATTACTCCACACAGTATGATATTTACTATAGCGTATGTAAGCAGCATATCCTGAGGTCTGAATTTCGACATTAGCATAGTACCTATCCATCTGCCGCCAAGGAAAGCCAGCATATACAGTCCGAAGAATGTGGTCGCCTCATCCTCCGACAGACCTGCATACATGCAGCAGTAAACTAGGAACAGGCTGTTGATGGCTGTCTGCCCTCCGTTATAGAAGAACTGTGCGATAACTCCCCATCTCAGGTGTTTGCGTTTCAACACTGCAAAATTGATAAGCTTGCCCTTCTCGCCGTGCGATTCCTCCTTGTCAATATCAGGCAACTTATACAGTGCAAACACCACAGCAAGAATAATCAGCAGGACTGCAAGAACCAGATAAGGCATCTTCATGGAGTCTGTCTCCATCTGAATAAATCCGTCCCAACCTCCGGGAAAGTCGGCAGGCAGAGTCTCGCGAGTATAGTTCTGTCCGGTAAGTATAAGCTTACTCAGAAACATTGCGGATATGAAAGCACCAAGACCGTTGAACGACTGTGCAAGATTCAGTCTTCTTGAAGCCGTATCGTGTGTACCCAGAGCTGTCACATACGGATTGGCAGCAGTTTCGAGGAAGCACATTCCCGTTGCCATGATGAAGAAGATTACAAGATATGCCCAGTATTCCTTTATCTCGGCTGCAGGGAAGAAAAGCAGACCACCGATGGCTGCAAGAATGAGACCGACAATTATACCCGACTTATAGCTGAAACGTTTCATGAACATTGCTATCGGTATGGGAAACAGGAAGTAGGCCAGCCAATAGGCAGCTTCAGTGAACGAGGCCTCAAAAGCATTCAGTTCACAGGTTTTCATCAACTGCCTGATCATTGTAGGCAATAGATTACTGCTGATAGCCCACATGAAGAACAAGCTGAATATCAGTAAAAGCGGTATAAAATATTTGTTTTTCATTCTGACATGTTTTTAATATAAGGTAACTCAGGCAGATTCTTGAAACCGTAAAAGGCTTTCGCGTTCTCGCCCAAGAAAAGTTTTTTGCTTCTCTCTTCCAATTCTTTTGATTTAATCACAAAGTCGTACGACATCTTGTAGGTAATGGCTGTGATTGTGCGTGGATAGTCGGAACCCCACATCAGTTTCTCGAAGCCAACAAGGTCGGCAGCTTCGTTGATGGCTCTGACAGCGCTGCGGAACGGATAGAACTCGTCATTGAACAGCCAAGTGATACCGCCCGACTCAATCATCACATTCTTATGACGGGCAAGCATTATCTGCTTCTTCCAATCCGGTTTAGTCACCATACCGAAATGCCCGATGGCAATCTTCAAGTACGGACATTCTGAAATGATTTCTTCCATCTCGCCCACCTGGAGGTCTCCCTCTGCCATATCTATGGAAAGAATCACCCCCTTGTCTTCCATTAGATGAAACATCCTCATCATCTCGTCCGAGTTGAGCATCACCCTACCGTCCTTCAGTTGCAGGCGGTGTCCCGGAATCTTTATGGCCTTGAACCCTTTGTCTATAAGTTCAACCGCCTGGTTATAGAAACCCGGTTTTCTGAATTCACACATACCACACACGAAGAACCTGTCCGGATATTTCGTCATCACCTCCATCAGATAGTCATTCTGAATGCCGTCGATATACTCCTGTGTGACAACAGCCGCGCCAATCAGGGCATAATTCATATTAGCCAGGAAAACCTCAGCCGTGTTTCTTCCGTCAATCATAAAGGGGGGGGGAGCATTTGTCTCACCTCCCCCATAAACAATGATTGACCGTTCTCTGTAGTCTTGATTTTCAGGCCATCTACTTCAGTGTCCTGATAAAGCCACAGATGCGAATGGGCGTCAATTATTGTATAATCCATAGAAACAGTATTTATGAATTTGCCCAACTTACTCTTTGCTGATCGCCTATTATCTCCTTAACCTTTTCCACAAGGCTCCAGTCTATCGGTTCCTCAATGTATTTTATGTTCTGAAGCACAGACTCTGTTCTTGCCGAGCTGAACAATGTTGTAGGTATTCTCGGATTGCTTACAGAGAACTGCACCGCAAGTTTCTCGATAGGGTATCCCTGTTCAGCACAATACTTGGCAGCCTTTGCACACACCTCAATCAATGGTTTTGGAGCCGGATGCCATTCAGGAACACCTCTATGTGTGAGAAGTCCCATACCGAACGGCGAAGCGTTTATCACTCCCACACCATTTTCGTCAAAATAGTCGAGGAAGTCCACCAGCTTGTCGTCGTTCAATGAATAGTGACAGAAGTTAAGCACCGCCTCTACTGTACCCGGAGCGGCATGGTCGATAATCCATTTCAGGTTTTCGAGCTGCAGGTCGGTGATACCCACGTGGCCCACCACGCCTTTCTTCTTCAGTTCCACCAGAGCAGGCAATGTCTCGTTCACCACCTGGTTCATATCCGAGAACTCAACGTCGTGAACGTTGATAAGGTCGATATAGTCGATGTTCAGACGTTCCATACTTTCGTAAACACTCTCCTGAGCGCGTTTGTCCGAGTAGTCCCACGTATTCACACCGTCCTTGCCATAGCGTCCCACCTTTGTAGAAAGGATGAACGATTCTCTTGGCAATTCCTTCAGAGCCTTACCCAATACGGTTTCGGCTTTATAATGTCCGTAATATGGAGAAACATCAATAAAGTTCAGTCCGCGTTCCACTGCTGTAAAAACAGACTGTATAGCGTCACTTTCTTTGATAGAATGAAAAACTCCGCCCAATGAAGATGCGCCATAACTCAATACAGGAACCTTAAGTCCTGTCTTTCCCAATTCACGATATTCCATTTTTGATAAATAATTTAAAGGTTAATATTTTTTACTCTGTTTATTCTTATTCATACAGATAGAACATACGTTCCATCATCTTCCATTTCTCGTCCGATGTGGCCCCCTCGGCACACTGCTGGAATTTGGCTACGTATTCTTCCCATTCGGCCTGACGCGGCAGAGTGGCAAGCTTTGCCATAGCTGTATCCCAGTCAAAATCCAGAGGTGTTTCCACTATCATAAAGAGTTTTGACCCCAATATGTATATTTCCATTTCCAGGATTCCCACCTCGCGTATTCCGGCGCGTATCTCAGGCCATGCCTCTTCCTTACTGTGAGCCTTTCTGTAGGCTTCAATCAATTCCGGATTCTCACGCAGACTCAATGTCTGACAGTATCTCTTCACAGGCAGGGAATAACTTTTCACTTTATATCCTTCTGTCTTCATGATATTATTGATATTAATATGTTAGTATTACATGTCACTGTCTTTATCTTTTCGACGATGCTAAAGTATGAAGTATCCATCAAAACAATAGAGGAGATTTTCAAAAAAGAAAGAGGGGATATTATACCCCCTCTTTTTCGACATTTTTACCCCTCATAAAGGAGATAAAAAGTCACCCCAAACTCTATAAAAAATCAAAACAGATTGAACTGCATTCCTGTGTAGAAAAATCCCTGGTTGGATTTCGGATTCCAATACGTCATCACCGTCAACGGGATTTCATATTCCATAATCCGAAGTTTATAAATCACATTCAGGGACACCTGAGTAATTCCTGCCGATTCGGCATACATGGTTCTGTTCACCATTTCCCCGCTTTCATTTCTTGAATTTCTCAATGCGAAAGCTGTTCCAATACCAGGACCGACCCTTAGCTTTTCGTTCTGATAGATGGTATAGCCCACATATACGAAACTGGAGTAGATGTTCTTGCTGTTGTCCAGATCCCTGTCGCGACCGTAAACAAGTGTAGAGAAGCTCAACTCCAGCGGAAATTTCCTGTCGCCCGTATAATTGACCATGAGATCAACGAAACGTCCAGTTTCATCAGGCTTATAGTTGAAGAACTCCTTATTATTATATGTAGCCCCGGGCGAGAAATTATATGTATCTATAGCCTTTATCTGAAACCTGCCATGAGTATATGCTATATACTGGCTCAGCTCCTTATAACTCCCCCTGGTGTTCGATCCGCCAAGGAAACCGGCGGTAAACCTCCCCGATGGGTCGGAAACCGACAAATCGGACGAGAGAATCAGTCCGTCGGCCACTTCAATGCCACGCCATAGAATCATGTTCTGTAGAGTAGTACTGAAATGAAGCTGAGCCTGAACATTTGCTGACAAAAATATAAATACAGGAATTAACAGTCGCTTTTTATACTTACAGGTATCCAATGATAATATATGTATCATACTCAGAGCAGTAGAAAATCGGTTTTAAATTATTATTATGGATTTATTTGTCGAAATACTCTATAAGATTATAAACATTCCAGTTAATATCCGACATGTATTTGGTCAATGATGTATAAGGTTTATAGTTATAATCGAGCATACCTTTATTGCAATCCTCATCATCCAGATACTTGAAGAAAACCCATCCTACACAATTCTTGGCTTCGAGCAGTCCCAAGGTAAAATGCTGGTAAGCGAATCCACGGTTTTGCTGGTCGCGTACCACGAAACCAGCTCCACTTGAATTGTCAAGCTTAGTATCCTCACCCTTGGTATAGAATTCCGTTACCATGAAAGGAGTACCGCCCGCCTGGTTCTTCCAGCCATCCATGTAGCCTTTTTCAGGCGACCATTTACTATAATAATTTATGGAAATGACATCACAATATTTTCCCGCTGCCTTAATTATATAACTGTTGTATTTAGGAAGGCTGTGCAGGCGTGAACCCAGATAAAGCAATTCAGGATCCTTCGATGCCTTAACCGCATTCTTTATGGCAGAATAATATTTTTCCGCACAAATACCGGCAAACTCATTGTTCAGTTCATCCGTTACATCAGAAACATTTGCACTCTTGTCCTTATCCGTCATAAACTTGGCGGCTGCAATATAAGCAGGATCCTGCTTGTTTGAAATTTTCAGGAATCTGTCGAGCAGCCTGTTTCCCCATGTAGAGAAGTCTATCTCATTATCCGAGAAGAATCCCAACACATCCGGGTTGTTTCTGAACATGCCGAAAGCATCCGAATTGAGATACTCCTTGCACCATTCATCCCATCCATCATAAAACACAAGACCTATCTTAAGATTCACGTTCTGCCCCGGATAGCTAATTCCCTTGCTATTCTTGAACTCTGCAAGGAATGAAAAGGAAGGAGCCTGTGTCAGAGGACTTGAAGCCGATTTATTATAATCATTTACAGCCTTGTCGCCTTCTTCCTTACCGAAAGCGCAGACACTATGAAATCCTATTTCAGAGAATTGTTTCTGCGACTTTGCCACCCAGTCATCTACTGAACTGTAAAGCTTGCCGAAAGCTGAGCTGTTGCCATCCATTCTGAATGAGGCGATACCCCTTACATAATATGGATAACCTTCGGGGTCGACTATCCAACTTCTTCCATTTGAGTTTTTCTCAACCCTGAACCGTCCAGTAGCCTTGGATTTTTGCCCTTTTGCGTATGAGCCATATTTATTCACGCTTTGCAAATACTCATCCTGTGTTTTTGTCTGCTGTTCATAACCAACCAGGTATGGCAATATCCTTGTCTTTGCCTCTATAAAAGCCTTGTCAGGTTTTTCCGCATACTCGACAATTATCGGTTGATACTGCTTGGTGCTATTAGGATAGGTTTCAGCAGGACCGGGAACAGGCAGTTGCAGTTCTACATCATCATCGTCATTATCGCCTGCATTGTCGCCGGGAGTATTATAGTCCTCCACATTCCCCGGTTGTGAGTAAATAACCTCAGGCGGAATATATGAGAACTCCTCCTGAGGGTCTTCACATGACAAAGCGAAGAACGGAACACTCAAGCAAATGGTTTTAGTAATAATAGTAGAATATTTCATTGTTGCAAATATTTAGTAAATTAATATAAATCCCATGTCCTGATTGTATCCCCCCATCGGTGGTCTATCGGGAACTCCATTTCTCCCCATGCCTTAACAGAAGTCCAAGGTTGGTCGGCATCAGTCCAGAATGGGTCAGAGGCAGGCAATCCCAACGGAAGGAATGCAAGTGTAGTCATATACAGGCTGCCATTGTTTGTATAATGATTCGAAATGCCAGTCTGATGTCCGCAGAATCCTATGGTGAGGAATCCGCCCTCATTGAAGTTATTGCCCGACTTGAACATACGTTTCATACACGCTGTCAGCGCACATCTCACCTGTGCTTTCGATACTCCCGCCGGCAACTCATTATACCATGCTATAAGAGCCAGTGGCTGCATTGTTGCCATACGGTAAGGTATAGAGCGTCCGAAAACAGGGAATGTTCCTTCAGGAGATATGAAACGCTCCAGAATCATGGCGAACCTCTGTGCCCTCATCAATGCCCTGTCATAGTACTTGCGATAGTCGAAACGTGTCCTCACGCCCGATTCCATTATTGCATGTATAGATTCGAGATACATAGGATGGAACACATAACTGCTATAATAATCGAATGCAAAGTGCTGTCCGTCTGCGTACCATCCGTCGCCTACATACCATTCCTCCACCTTGCGGAAAGTAGAATTTATACGATATGTATCCTGTCCGGCATCAATTTTGGCAAGGAAGCTTTCAATGGTGGCCGAGAACAGCAGCCAGTTAGTGTAAGGAGGGTCAATGCGTCGGAGACCTTTGAACTCTTTTATGTAGCGTTCCTTTGTTGTCTGGTCCAGCGGTTTCCACAGCTGGTCGAACGCGCGCAGGAAACTTTCCGCAATATAGGCAGCATCAACCAGTGCCTGACCATGACCGTTCCACAACAGATAATCCGGACTATTAGGGTCCACCGCATTTGCATAACTCTTCAATGCCCATTCTTTCAGTTGCTTGCGCTGCTGTCCTTCTGCTGTATCATCGTCAGGCAGGCTCAACCATGGAGCTATACCGGCCATGAGACGTCCGAAAGTTTCCATATATGCAACCTTCTTGTTACGGTTATCCCAGTTTGGACTTACCTCAAGAATCATATTTTTCTGCAGTTCCCCTTTCGCCATATTGCTCAACACAGGAGCAGCCATCCTGTAAGCCATATCCGTCCAGTATTTTCTTGTCTCGTTGTTGTTTGCCTCGAGATAACGCACATACTCGCAAGCGGCAAGAAGGAATGCGCCTACCCCAAAGTTGGCAGTCGACTTGGCGTCAACCACCTGTCCCGGAATAGCCTTTTCACCGATTGGCTGGACATAACCCACCGACCAGTCTTTCTGCAGTGCAGTCTTGGTAAGATATTTCCATGCTTTCCCCACTACAGGCATAAATTCATCCTTGTCAAGATAACCGTTGTTTATCCCCCAAAGCATACCGTAAGTGAAGAAAGCGGTACCGCTTGTTTCCGGTCCCGGAGCATGTTCCGGATCCATCATACTTCTTGTCCAGTAGCCCTCCGGCTGCTGCAGACATGCAACCGCCTTTGCCATACGCACAAACTTATCCTCGAAAAAAGACAGATGCTCATAACCCTCCGGCAGGTCCTTCAGCACCTTTGCCAGAGCGGCAAGCACCCATCCGTCGCCTCTTGCCCAGAAATCCTTCTTTCCGTTCAGACTCTTATGCTTGGGATAAACATATTTTGCGTCGCGATAATAGAGTCCTTCCTCCTCATCATACATTATTGAGTCCGACGTACAAAGATATTCATACAGTTTCTTAAGATACCGGTGATTATGCGTAATCTTATACATCTTCGTCATTACCGGCATCACCATATAAAGTCCGTCGCTCCACCACCAGTAATCCTTACGCGGTGTGCTCATCTGGTACTCCATGACTTCGCGTGCACGCTTGATTTTATAATTCTCCGGCATGACGTTATACAAGTCCGCATAAGTCTGGAAGCACACCTGATAATCGCCGAACAGCACATAATCATCCTTTACCCCGTATTTATACTTCCATTCAGATTTGTTGTTGCTTTTCGCACCCATCCACTGGTTATACTCAGCCCATGCCTCCGAATACTTTCTGTATTCTTCTTTCCCAGTAAGGAAATAGGCTTCCATATTACCGGTGTGATATGCCGCATAATCCCAGAAAGACCTTGCTTCGGGGGCATGATTTTTCTGCCAGGCATCGTTCACTTTTTCAATCATCTCCCTAACTTGCTGAGCCTCAGTTTTTTTTTGCGAAGGAAAATGAAGGTAAAACAGCTATAAGGATGTATAACATCCAGTAGTATCTATAACAGTTCATCTTTGTGATATTGTTTACATTTTCTAAAACGAAATGGGGAAGAATATATATTCCTCCCTCATTTCACGAATAATTGTATTATTATATTTATTTGTTAGGAGTCCATTCTGCTCCGTTGTTGAAACCTTCTGTTGTAGAGTCAAAACTTGCATCTGCTCCTGTACTTGGTCTTTCTGTAATTTCTTCAATCTTAAAAGAAGTGATTTTAGCGGTTCCAGTAGCATCAGTACCACCAGGGACATTAGTCTGTACAGTTAAAATAACGTTCTCAAGAACCGGCCACACAAGTGAACCATCTGCTCTTGAAGCTGGAGTTTCAGCAGAAGTAGAACTACTGATTGTGAATGTATTTGTATAGGTTCCACTTCCGGTATTTCTTCCAATCCAGAATTTATATTTATCAGATGCTCCCAATCTGAATGTTGTTGCACAGTCGTTAGATGCGTATGTATAAGTAAATTTGTAAGTACAACCATCACGGAATGACATTGATTTAGTAACTGGGAATTGATTATCTGCTGGAACAATTTCCAATTCTCCACTTGCATTAATTTTTTCGGCAACTCCTTCTGCAAGATATTCCTTAACTGCATCTATGTTAGCGAAGTTAAAATCAAAAGCATCTGCATGAGTCAAAGCAACATTGGCAGATTCAATCTTGATATTAGCATCTTCGTTGTTTTCGTTTTTAGCAGTCAAAGCACTAACAGCATAATCAGTGTTATAACTTACGCTAATATTTGCGTCATTACTATAAATCTTATCACCAAGAATAAGAGTCATAGTAGTTCCATTCACAGAACCGGAAGCAACAGGAATTGTTTTTCCTGCTACTGTTATGGTAAATGCTTTGTTAACAGCATCAGTGAATGTTCCAGAAACTTCCTTATCGAGTGTAAGTTCAATTCGGTCATTACCTGTTGTCTGATCAGGAACAATTTCTTTAGCTGAAGAAACGGCAACAGTAGTTTGTTTTTCCAAATCCACAGGAGGTTCACCGCCTCCTTGATCATCATTCAATACTATCGTTACAATCTGTCCTTTAGTAACTATAAGGTTTTCACCACTGAAGTTATAAGTTTTAGTACCAGAATTTCTTGTAAGTTCTAAAGTAAATCCATCGGTAAATGTCACCGGAGCTACAACCATTGAGTATTCCTTGGCATTTTTATTTTGTTCATTAGGACCAACAAATGTTCCCTCTTTAGCGGTTAGAGTTATAACATTAGAACCGGATTCCACTGTCAGGTTTGCTGAAGCATCAATTTTTACGTTCCCTGCAATCTTTACATCACCACCAGCAGTAAGTTTAATACCTGTAAGGTCAGTAAGATTATTTTTAAACTTAACCAATCCACAAGTATTCTGGAAAGTTAAAGATTTGTTATTATCTGTTGCAGTAGCATAAGATATATTTGCATTTGCATCGAATCCCCAAGCCGGAGCTGTCTGTTCAGATGGCAGTGTAGTAGTTACGACACCTTCAAGACACACAGCTTCGGCATTATAAGGATAAAGAGCTGTATATGAATTGTTAGGTGTAGCCTTACCTGTAAACGTTGTAACTGTGCTACCACCTGTAGCGGTAGTAAACTTGTTATTTTCTTGGCCTGAAAAGATATTGATTGCATCTCCTGTTGTCCACCACACCGTTGTTCCATTCTGCAACGAACTACGGCTTGAAGGCGTACCGGCAACAAAAGTCATATCCTGAGGACCACTGACTGCATTTACATTCGACAGTTCGTCTTTTGTACAAGACTGGAGCATTGCAATACTCATCAAAGCCGCTCCACAAAATAGCATCGTATTTTTCATGACATAAATTATTTGTTAAACAGTTTCAATAATAAAAAATCACATCACTTGTTATTCATATTCTTATTCTTTAGGATCAGGTTTCCATTCAGTACCGTCATCTTCAAAATCATCATGACCGCCATCTACAATTCCGGGAGGTATTGATATTCGGCATACCGCACTTTTTATTCCATTACCCGTATCTACAGAAGCACCGATATTAGAATCTCTGCCCCCGTCGATTGCCACGACCGTACATCTCATTTTATCGTCCGATGGTGTAATCATCAACACATCAGGGAAAGAAGTTCCCCAAACTATTGACTTGTAACCGGTATAAGGGAGATTATCCTTGGTTATATTAATACCCAACTCCACAGTGCCACTATATGGTAATTCTATATAACTGACAGGTTTGTTGTCAGTCTGCCCATCCTTGAACACTACATATTCAATTTTTATCTCCTCAGCTGGTGTTCCATCACCTCCACCTACGCCATCATCATCCTTATCACACGAGATTGCCGTAAACTGTATAAAAAGAAGTATGAAAAGGTTGTATACTGACAGAATCCGTGGTTTTATATCAACCATAATAAAATGTTATTTAAGCGCCAAACAAAATTTTCAATATTCAAAAGGCATAAGAGGAAACCCTGAATATGCCTTATTACCATGAAAACAAATCAATCTACCTTTTTCAATCCGGAATCAGAAAAATATGTTATTTATTTAGAACATATTTTTCCGATTTGCCAGATTACAATCACAATAAATAAATCAACAACTAAATCTAATTACCTAATCTTATAACTAAACCCTCAAACAATGTTATTTAACCTTTTCTATCTTGACATCATCAAGCAGGAAGCATCCACCATTACCTGAACCCGGAACAGCTGTGAAACGATATACAAAACCATTTTCCTGCAATTTGAATTTAACTGTTGTAAGATTGTAATTCTTACGGTCTTTCTTGACCTCAGCAGTGGCAATTTCTTCCAGTTTCTTTGAATCCGGATTATAGTACTCAATCCTGAAGTTAGGTTTGTCACCCCAACTGTATTTGGTATAAGCTGAAATCTGATATTCTGCTCCAGTTTCATAGCTGATGTTTACAGCCTGCCACATACCAACCTTCACCTCAACAGCATAGTTGCCTGAATGTGCCTTTTTCGCATCAACTATTTTGTTATCTTTCTTTTCCCAGACATTCCATGATGTCAAGTCACCTGACTCAAAATCACCGTTCTTAATTTCCTGAGCGTATGCAGAAGTCATCATCATTCCGCAAGCCATCATTGCTAAAATTTCTTTTTTCATTTTTTCTAAGGTTTTTAATTTAAGTATTATGTTGTATCTATTAAAATCACTCTTCTATTGGAACCAACTTATAAGCCCTGACCCAGTCATAATAAGTAGTACTTTTGTCCTTATCCTTCAAGTCCTCAGCTGTAGGTACTTGTTTTTCCCAATCGTATGTTTCAGTAACTATATGTATGAACATAGGTCGGTCAAACGGAGTATCTGTATATTTTGTTGTAGGCTTGATAGTGTACATATACTTTCCGTCATAATAGAATTTCACGGTATTTGCATCCACCCACCAACAACCGTAAGTATGGAAATCTTCTGCCGATGGGTCCGTCATATACGAAACCACATCCGAACGTTTCGCCGTATTGTCAGTACGTTTGCCTCCTTGTTCCTGATACCAATAGTGAGTATTACTGTTCATCTGCATATTCCATGTCTTGTTCCACGGATTATCAGGGTTGACACTTCTTATTATACCCATTGTTTCTATAATATCAAGTTCCTGACTGCTCCATGTCTTTATCTTCTTGCCGCCTTTCATTATTTCCTTCATTACCGGGCGGTTGGAAAGCCAAAAAGTAGACGACATGGTAGTGAGCGAAGCCTTCATCCTTGTTTCATAATACCCATAATGTGCCTGGTTCTTTGCAGAAGCAACCGCTCCACCGGCAAGACGATATTTATCGCCCGGCTTTCCATCAAGTCCTTCTGTTGGCGACAAAACGGTATTGATTATACGAAGACAACCTTTCTTGACACTAACATTCTCTGCCTTGAAAGTTGCAGGCGGCCGACCGTTAGTCCAATAAGGACTTTTAGCATGCCATTTAGCGGCATTAAGACGTTTACCATTGAATTCATCAGTATAATCTTCGTTAACTACCCATTTATAACCCTCAGGAGCCTCAGGCAAATTTTTTATATGCTCTTCAGCCAAAGAATATTCCTTATCATTTTTTAATGTATAAGATGACAGGAATAAAGATGCAGCAGATAAATACAATACTGTTTTTCTCATAAACTTTGTCGTTTTAGATTTTTTGTTACACGACAAAAGTATATAAGTTTCATGAAAGCATTAAGGGGGATTTACATCGTAAAAGGTGGGGTAAAATTCTACCACTCCCTGAAACACAATTATTTCACTCATGAAACCATGTGTTTTTACGATATATAAAACCCGACAGAAGAATAATACCGTATTACCGGCTAATTTACATAAGAATAACTTTTCAAACCGCCATATACCCCACTTTACGTCCGTACCCTCAGTCCTCGACTCCGGCAATATGTTTTCCATATCGAGATCTATGGTTTTCTGCCTCGGATTCAACCACTAACTGTCGAGCATGTGGATTGCGTATCTGTCATAGAATCTCTTTCCGAACCATATTATCTCGTCTGTGCTAAGTATGTTGTTCAGACGGATAATCTTTCCGGTATTTTACCACCTACTTCTCTTGCAAATCCTGATCTGATATAACCGGATACTCTCAATTCATTGATTTCCGACTTGTATACAGTCTGCGAAGAGGCATTGAAACTACTGCACAGACTGAACAGCAGCAGGGGAATAATTTAACTGATTTTAATAGTAGACATTCTGTGTTCATAATATTTCATTTTAATGATTACGTTTCTGACTTTCGTCTGATGCAAAATTATGAGGTATCGGACGGGGTTGTATCTTTCAGTAAAAATCAGTAAAGTCTTGGCAAGGGGTAAAAAACTTAACATCTTGTATATAAATATATTACAAACAAGGTGCAAAGATTTTCAGTAAACGATGGCGAATACAGAACCTATATATTTACACGCCATAAAATGAAGAAAAAGCAGTAGGAAAAAAATGCGGGCAAGTTCCGGATAAAATGTGGGCAAGTTTAAGGTAAAACTTGCCCGCATTTTAGATAGAATGCGATCGCATTTAAAACAAGTAAAAAACGAAGAAAAAAAATATGTGTTCTTCACAGAACACATATTTCAAAAATAGGTATAAACACGCTAAACAATGTTAACAAAATCTATTTATAAAAAAAGCTCACATCAATAATATCTGCAACATTTTTACAATACTCCATAAATGAAGAGACCTTGGGATGATTTATACACAGAGCTATCTGTGATGTAGGCGAAAAACGTCCTGTCCCGTCAAGAAACGCTGTAAGCTCAGATGGGAGGAGTATACTGCCAATACCTGGATTTACGTCAGTCAGAACGACTGTATTTACAGCTTCCACCGCTGACACATCAAGATAATCGAGTGCCGGAAGATCTGCGAAGTGCAATTTTCCTATCATATTGCCGCCTTTGCTGCCCTGAAGAGAGACACTCTCCAATGAAGAACAACCGGATATATGGATTTCACTGTCGAATATTGAAGTTTCGGAAACATCATCATTAAGTATAACAGAAGGAACAACTACCAATTGAAGCGAACTGTTATTCTCCACCCTAAGTACTTTCAATGATGATGCGGAACTTAAATCCATTCCCAAAGGTGTATCAATATTAGAGATTGAAAATACTGAAACTCCCGAAGACGGCTTGACATACGACATGGAATAATGCTTGGACTTCACTCCCGAAATGTCTACTTTTCCTCTGAAACCGGGATTTGACAATATATACTCCACACCCTCAAGGTTAGCTGTCTGCGACAGGAAAATGAGGTCGTTCCCTTCGGTTATCCTCTTCGTGACATCAATCTCCAACGATGAGACAAACACCGACGGGAAGTTTCTGTAAAGATATGAACGGAGCAAAGGATCCGGTACTCTTCGGTTTACTGTATATTCAGTGTAATTTCCATCCTCGTCCGACATCACGACAAGACATTTGTCCGTCATGGCTTTATAGAATGCAGGTATGACATCTGTATTCCATTTTGCAAAGTAAGGAAGTTTCAGATTTGTAAGACTTTTACAAAGCACCGTAGTGCCATCGGTTGAAATAAGATTGAGATACGAAGTTATGCCGTCATTGCCGCGTAAAGCTACCGATTTTATTCCTTCGGGCAGGTCAGCAAAGTCGAATATAGAAAAACTGTTACACTCAAGATTGACATCTGCGAGCGAAGGAAAACTCCTCAAACCGCTAATAGATGTAAGTTCGCATCTACTCAAGTCCAAAGAAGTGGTATTGAGAACTTGATTGTCACAAATCAGCTCTCCGTTTTCGCTGAAATTAAATCCTTTCCGGGTCAAGACATCGCGTAACTTTGTATCAAAAGTCACTTCAGACACTTCAAAGTCGGAAATTTCTGTTTCATCCTTACACGAGATTATTGTGAAACAGAGAACTATCAGTACATAAAAGCTAATAAAATTCCTCATAACAATCAGTTTTGTGGTAATAAGACTATATTATCAATCCAAGCCGCGTCGTTCTGTCTTTCGCACACAATGGCACACACTACTTTTTTCACTGTAGAATTAAAATCGAAAGATACGGCTTTATAATTGCCGGGAGAAGAAAATTCCTCTGTATATACCGTTCCTGTAGACATATCCTGTAGCATGACTTTCAACTTACATGCTCCTTCGGTCTTTACATCAGCAGAGAAGCGATAAGTCCTGCCACTCTCCATGTCAACCCTCTGCATGAGTCCTGCATGACCAGATATACAGGCTACATTATTGCCTGCATTGTCAGTCTGTACGCAAACCGTACCATAGTTACCCAATGGCTGCCATGCTGAAAGTCCTTCGCTGAAGGTTCCATTCTGCAAGGTAGAGACAGTATATTTCTCAACCTGCAGTATCATGGACGATACGTGACCTCCTCCGTCGGAAAAGGTGATGTCGACATTATTATCGCCATTCTTCAGCAGCTGTATGTCGAACGGTACTTCTATCATACCGAAAAATATATTGCGGTTGCTCTGGCCGTAGCCTTTCCAGTTGTCGGGAACACTCACAGCGGTACCATTAATCTTTACCACCGGTTTCTTGGAAGCAGAGACAGGACGGCCTATCGACATACGCAAGCTTGCTCTGCCCGAACCGGACTCGATTCCTGTGAAGGGGAACGAAAGGGATGATCCGGCGGAAATCGGTTTCAGATACTCACTGCTGTAATATTTATTGCGGATTATGGAGTTCGTGAATGCTGACGAAGACACATCTGCTACAAGGACTATGGTCTGATTTGGGACAATTGAGATGCTTTCAGGCATGGACGGGACATTCTGTTCCGTATATTCTATACCTGCGTTATAATTGACATATAGAGAACGCTTTGTGACATTCGATACATCCTTCCAGCTATTCTTATTGTTCAGATATACAGTCTGCGGGTTATCATCAAGATTATCAAGGGCGATATAGAGTCTGCCTCCATCCTTGAATGCCTGTACCTGAATATCAGGATTACTGCTGGTTATATCAACACGTTCGCCTTTTACATTCTTCCAGAGTTCGAAGAAATATTTTTTGTCATTAAGCCTCCATGTGGTATTCTTCAGATTCTGAGGATTGTCGGGAATAAACAGTGCCGCACTATATGAAGTATAATTGTTTGCAGCGGTGATATGCCACTCAGCCTTATCTGAGACAAAAGGTATTGAGATAAACAAATTGTCCTGACGTTCCATCAGATTAAACAGAAAATGATTAAACGACGAAACACTCCGCACACTGCTTATGTCATCATAGCTGTCGTCGGGCTTGCTGTTGTCAATACCTCCAAACTCGGAAATGGCAAGAGGCTTGACATGTCCGAACTTAATATAGGAATACGCCTCAACCATATCAAGAACTGCTTCGGAGTTACTTCCTGAACGTTTCGTATCGGTGCCGGTTACATTTATTCCATCATAAAGATGTACAGAGAATCCATCCATATATGCACCTGCCCGATCGATGAACATTTTCATGCGGGTGTTCCAGTAATTGAAGTTCCCATCCTCCCAGGCGGGGTAGGCTGCGGCATAGCCTATCACCTTCATCTTTCCGTTAAGACGCGGATTATTGTGTATATGTTTACCTATTGAAGCATAAAAATCGACCATCAGTTCGCGCATAGCCTGTCCCTGAACGGTAAAACCGGCATCATTTGCATGAACGAACGGTTCATTGAGGGGTTCAAAAAACTCAGGTACCAGCTCGCTGTTGGAATAATACTCAGCCGACCATGCACCTGCAGCCTGAACGTCTATGCCGCCCTGTATGTGCTGTACATAGGGATGCTCTGTGGCAATATATCTTTTTACGGAAATATTTCCGCTGTATGGTTTCATCTGAGGATATTTGCCTACCTCATGCGTCTTGTTATACGCATACGAGTATGGTCCCCAGAACTTTCTTCCAAGACCGACCTGATAGTCGGCAAGAAACTTGCCTACATCCTTATCATCATCGGAGGTGGAATGAATATTGAAATATTTAGAACGGTCGAGTTCTGAAACACCGCTCAAAAAGCGACGGGTATTATAGTCGACAACCACCTCGTTCCTTTCCTGACAATAAATACCGGGAGGAACACCTAGGGTAAATGCCGATAACAGAAAAATATATTTATAGCTCATAATTTCTTTCCTTTTAGACACAGAAACTTGTCAGTCCTGATGTGGATACATTATTTTCTCACTTTCTTATCGTAGCGTTCAGTCTGAAGAATCATAGTAGCCACACGGCCTCCATTATCCGGGAATGTTACTGACACCGAATTTTTTCCTTTTCTGATTAACCGGTAGTCGAAAGGTATTTCTATCATACCGAAGAAATCGTCTCTGCCGGTCTGGTCATATCCTCTCCAATTGTCGGGCATGTCGACTTTCTTGCCATTAACCATTATTTCAGGTTTCTTCGACATCTCGTGCTTCCTGCCTATTGACATACGCAGAACAGCTCTTCCTGTACCCGGTTTCAGACCATCGAAATCAAACACAATTGGTTTTCCGGCTTCCACCGGCTGAAGATAAGTGTTGCTATAATATTTAGTACGAACTATTCTGTTTGAATACTTTTTACGGATGATGTCGGCACACAATATTATTGTCTCATCTTTTATAATGTCAATACTTTGAGGCATCGAGTTCAGCGTCTTTTCATCATAAACTATACCTTTATCGAAAATCATCTTCAAAGAGCGCACAGAAACATTATCTACACCCTTCCAATTCAGTACGTTTTTCAAGTTTACCTTATGTGTATAGTCATCAAGATTGTCGACAGCTATGTAAAGCCTGTCATCGTCCTTAAAAGCTGCCACCTGTATGTCCGGATTGTCGGAAACAATATCTACACGTTCGCCTTTCACATCCTTCCATAACTTGAAGAAATATTTCTTGTCGTTCAGTTTCCATGCGGTATTCTTCAAGTCGTGAGGATTGTTGGCAACAAATAAAGCAGCTCCGTATGGTTCGAAATTATATTGTTTCGTTATATGCCATTCGGCCTTGTCAGAAACAAAGGGTATTGAGATGAGCATCTTGTCTTCGCGTTCAAGAAGATTGAACAGTATATGATTGAACGAAGCGACAGTTCGTACAGAGGCTATCGGATTATATCCTTTGGAAGTGTTGTCTATTCCTCCATATTCGGTTACGGCAAGAGGAAGAACTTTCCCCAAGCGGATGAACGAGTAGTTTTCCATAAGGTCGAGAATAGCTTCGGAATTACTTCCCGAACGGCGGGAACTCTTGCCTACTATGTTTATTCCATCGTAAAGATGTACCGACAAGCCATCCATGTACTCCCCGGCACGGTCAATGAACATCTTCATAGTATTATTCCAATGGTCGAAATCGCGCAACTCCATAGCCGGATATGCCGCGGCATATCCAATGATTTTCATTTTTTTCAGACTTGGCTCAGCGTGAATATGCTTTCCTGTCTGTGCATAAAAATCTGCCATGAGCATCCTCATTTCCTGACCATGCATATTGAAACATTTGTCGCGTGCATGGACAAAGGGTTCGTTAATGGGTTCGAAAAATTCAGGAACTGCCCCTTTCACATGCTTGGAATAGTATTCGGCAGCCCATGCACCCGCCTTCACTGGGTCTATGCCCCATTGTATGGTACGCGCGTTGGCATGTTCCGTAGCGACATATCGTTTTGTTTCCTTCAAATCAGTGTAGTTCAAAGGCTTTTCTGAAAAAGGATATTCGCCAACCTTTTTTGTCTTGCCATATGAATAAGAGAACGGTCCCCAGAAAGAGCGGCCGATTCCTACACCGTAATCTGCAAGAAATTTCCTGACATCTGGATCAGAATCTTTAGATGTGTGTATATTGAAATATTTACCTCTGTCAAGTGCCGATACATCATTCAGGTATCTCTGAGTGGCATAATCCACTGTGACAGTAGTGTTATAAGTCTTATTCTCGGAAGATGATAAAGGAAAAACCGAGAAAGACAAACACACAGACAAAGCTGTAAGAATTATGTTATTCATTGTATTATCAAAATTTAAAAGGCAGAGAACACTCCGATAGTTCAATTAAAGTATTCCCTGCCATTAAGATTATCACTTCTGTTTAAACACTAATATCAGAAATCGGCCGGTTTGAGTACATCGTTCAGCACCACTTCATATTCAACTTCTGTTCCGTCGTTTTCAGTAACAGTAAGATGGCCGTAACCGCCACTTGAGTTATTTTCTTTCTTACCTTCAAACATGAACATTCTCTTCTTCGTCACTTCCTGTTCTTCTTTATCGCCTGTTTCAGGATTGATAACTTCTTCCTTTTCAGTATAGACTTCATTGAAAGAGAATGAGAGATGTTTTTCTGTATCCGAATTGATTTTCAGCCACTCGGGCAATTCCGAAGGAGCCTCAGACGACTCGGCAAAGAACTCGATCTTATTCATTCTCAAAGTCTGATAGTCATTCTTCCAGGCAATGAGGTCGAACAATTCGCGATAAACAGAAAACTTGGAAACCTCGCCTGTCTTTCCTGTTTCCACATTCTTGAGATAGGTAAGTTCATACACGGGAGTAGAATCAAGTTCGACCTCAGCCCATTTGTCATTATCACATGCTCCGAACAAAACCAAAGCACATAAGAATGTAATTGTCTTATAAATTTTATCTATTAGCTTCATTGTTACTATAATTTATTATGGTCTTACTTCAATATATCCGAAAAATATATCGTCAAAATAAATATTATCCTTAAAGGCATTAAAGCGCATACTGAGCAATATATTGTCCATTTCAGCCTTTGAAGTCACAGTGGTTGTGGCCGACATCCATTTGCTGTCGGAGCCATTCACAATGCCGCACCATGGTCTATCGCTCTGCCATGTCATATCTTCAGCTCCTTCTTTACCTGCCGGAACGAAATACGGACTCATACCCTTACCCTGTTTATACCCCGGTGTATAATATTTGTAGCTGAAAGTATATGTACCTTTACCACCAGTAAATGTCTTGGAGAGTAATGCCCTGCATCGGTCAAATGCTTCGACAAACATACATTTTGCACTGTTGTTTATTCCATCCTTCAGAGGATTGTCCACAACCTGTGAAGGAACTACAGGATGTGTTTTGGTATCGGCATCAATAACTTTCCAGTCGGCATATGTGTCAGAATTTTCAAAATCTTCATCCAGGAACGCACCAAAAGTAGTCGCTACGTTTGGAGCTGTAGCCTTTATCTCAAGGTTCTGATATCCAACCAACGCTTCAGTTAAAGTTCCTGTAAGGGTCAGTTCATCTGTGTTATAGATTTTCTCAACCAAAGTAAGAATCAGTTCATATCTGCTTTGCTTGTTTACTTCTGCTGCTGTGATGTTTACGCTACCCCTGACAGCTGACGGTCTGTTATACGAGTTGGAGTAAGTAAGCTTTAGAGATGATGGATTTATCTCTTTATATCCAAACTCAGAATTATCCAAATCTATAGCAATGTGTGTTTGGTCAATCTGACGGATGTTATAAGTAATAGGATCATCACTAGGTACTACTGTAATAGCCAAAGGCACAACAAGAGTTTTTGGCGAAGCTTTTGGAGTGTACTTACCTTTACCCTCACTGGCAGAAGTTCTTTCTATTGTCATGGAAAGAAGCAATGGCTTATCGCTGAATTTCTTTGCAGTGAACTGGTATGGAGTGTCAAAACTGGTTAATTCGTCATTTACGCCAGTATCCGCACATTTGAAAGTCCATTTGTTAGGCAATCCGTATGAATCGTCCTTAATATAGACAGACTTACCATATTCAAGTTCGTATTTTTCGTATTCGGGAGCTTCCTCAGTTCCACCGACTATTCCGGTCTTTATTTCCTGTGTACACTCCGGATCACTGTATACCTTTACGGCCGGTACGAGGTTAGGATCATACACGCGGATATGGAAAGTTGTATCCATCACATATACATCACCCTCCTGCTTAGCATAACAATATTTTTTGATATATCCTCCGGTATTGTCGTCATACACCGAATATGGATATACAACCTGTCTGCGGAAAGTATTGCACAAACGTACCGTATGGTCACCGGGTTTAGTGAAATACACATGTATGGTTTTCAAATCGTTGGTATGAGGGATGGATTCATCAATCAGGTTTGTATAGTCTGTCTGTCCCCACTCCATCTTACCATTAAGGAACTTTGTACCATCATCCGACACAACCCACTGATGCGACAACATGCCTTGGGATAAGTCCATTATACTTATATAGTTATTAAGATTCAGCTGAATAGGTGAAACGTTTTCCTGATCTGTACTCACATGCCAGGTACATTCAGCCACGTTATTCAACGGTTCAAACTCATCATCCTTACAAGATGTCAGAACCGAGATTAATGAAAGAGCAATATATAAAAATCTATTTTTCATCGTATTTATTTATTAATATCAGGATTTGATGTAATTTCTATATTTGGAATAGGCCAGTATGCCACTTGCGGACCGTAGTTCAATGATGCTTGGAAATAATCCACAAAAGCGTTTCCTCTCTTTTCTGGCGGCAGCTCATAAAATCTGTACTGCTTTCCAAAGTTGAATGCTGATACCAAAGCATTAGGGTCATCAGGATTAGGCTTAAGATATTTGGTCTGAATCATACAGTACTTATATTCGTCGGATGCCAACTGATCAAACCTTTCCTTAGTTATATTCCAGCGTCTCAAATCAATGACACGTATGGCATGTCCTTCCATACACAGTTCAAGAGGACGTTCCACATACATCAGATGATTCATTACATCACTTGCAGCATATTCCTTCTCATCGTATGTATATCTCTTGAATTCTCCCTGTTCCGATTTTCCGATAAGCACAACTCCAGCACGGTGACGTACCTTGTTGATGGCATTGATAGCTGACTGAACATTTCCATCGCTTGCACCGCCTTTAATCAGACATTCTGCATACATCAGATATATATCTGCCAAACGGATAAGACGATAGTTTATTCCTGAGGCCATAGCAGGCTTAAATTCAGTTTCACTCTTACGTGTATCCCAATTTGATAATTTTCTGAAATACGCTGAAGAGCCACGGTTGAATTTTGATACCTGTTGTGGGAGAGACTGATAATATATCAGACTTTCATCGCCGTTTATTGCAAGAGAGGCAGATGCACGCATGGAATAGCTTCTGAGGCGATATGCCTGACCGTCTTCCCATTTAAATTCCGGAACTATGTCATCGTAGCCGGTAATCTTATTGTATAAAACTTTATTATCTCCGACAGTTGAGACGAGTCGTTCGCGTACTCCAACATATTTTCCTGCTGTTGCATCCCACGTATAAACGTACGTTCTGTTATATACGACACCCTGACGGTCCACCTGCGAGCTGAAAGTTGTTCCCAACTGGTCGTATATAATATCCCTATGTTCAGGATCACCATAATTGTCGGACTGCATTTTTATCCAGTTACGTTCATCAAGTCTGTCCACCGGCTCTGTTTCGAATGCTTCAACAAGCCAAAAAGCAGGAACAGTGTTAAGCCAGGCATCGCCCAAGCCATTTACATTCATTCCCCATATATTATATAAGGTAGACTCCGACCATGTACCGAATTCTGTATTATACTGTGTAGAATAGGAAACCTCGAGAATAGATTCCGAATTGAATTCATTGGCAGCAGTAAAATTATCGACTATGTCATCAACCAAAGCAAAACCTCCATTATCAATAATATCCTTAAAATATTCGGCAGCTTTATTATACTCTTTATCATAAAGGTAGCTTTTGCCTAATATTGCCTTTACAGCCCAAGAGGTGATACGTCCCAAATCGGTTTTCTCCCATTTGTCATTCAAGCCAAGGTCAAGAGCTTTCTGTAAATCTTCTCTGTAATATTTCTTGATTTCATCACTTGGTGTAACCTTTTTATAGTAATCTTCTTCTACCTCTGCAATTTCATTAATATAAGGAACATTACCATTATTGAATGAATTATTGAGATAAAAATAAAACAAGCCACGCAAAGAATATGCCTGTGCCTCAATCTGAGCAAGCTTGGTTATTTGAGGTTCATCTGTAACATTTGGACGGATTTTCTCTATACTGGCCAGAACCTGATTCGCACGGAACACACCAGTATACAGTGCAGACCATTTACCACGGACTGTTCCGTATGAATCATTAAAGGTTTGCTTATAGGCTTCGTTATCAAACTGCTTTCTGTCCTTATTACCTTCAACTGCTATATCACTTCTACGGTTCTCATCGAGCGGATGATAAATATTGGTATTTTTCAAAGCATTATATACAGCAGCCAGTCCTTTCTCGCAGTCGCCTATTGTTTTATAAAAATTCTGTGTTGTCAGCTGATGTATGTTTTCCTGCGTAAGGAAATCGTCGCATGAAACCAATGTCATGCCCGACATCAACAGACTGAATACTATTGTTTTATATCTGAAGTTCATATATTTATATTATTAAAAGTTAGAAATTAATCTGGAATCCGCCACGCATCTGGATACTTATAGGATATGTTCCATAGTCCAAACCACGACGTGACAATCCATTACTACCGACCTCAGGGTCGTATCCGTCGTATTTTGTCAGTGTAAGAAGATTATCGGCTGCAACGTATAAACGGAACTTGCCCAATCCAAGCTTTGATACCCAACTCTTGGGGAATGAATATCCTAACATAATATTTTTAAGTCTGACAAATGAACCGTCCTCAATCCACATATCAGTATGAGCACGATAGTTGTTATGCCCCTCTGTACGATAAGAAGGAATGGTAGAGGTATAGTTGGTAGGGGTCCACATGTATATCAGTTCCTTATTGGTTCTTCTTTGATATGTATATATCTTCGTACCGTTTATTATTTCATTTCCAACTGAAGCATACCAGTTCATAGAGAAATCGAAGCCTCTATAGTCGGCCGAGAAGTTCAAACCAAGTTCATAATCCGGCATACCACTACCGGCATAAACACGGTCGTCATCATTAAGAACACCATCATTATTGGTATCGATATACATAAGGTCACCCATACGGGCACTTGACTGTAATTTCTGATATTCTGCAAGCTTCTGTTCAGTATTGATTACCCCTGCGGTTGGCATAACAAAGAAAGCACCGGCTTCATATCCTTTCTTGATTGCAGTTACATAATCACTTCCTGATGAAACAGGTTTACCGTCGGGGAAGAAATATAACTCATTTTTTCCTGCCATAGACACAATCTCATTCACGTTTTTGGTAAATGTACCAGTCAAGCTGTAATTAACACCACGTATTTTGTTGCGGTGAGTAAGTGAAAACTCAACACCACGGTTTTCCATATCTCCGGCATTCAATGTAACAGTTGAACTCTGGCCCCCTCCATTTGACGGTGGCACGACCATCGGGAAAAGCATATTCTTCTTGTTACTCTTGTACAAATCAAGACCTAAGATAAGCTTGTTATTATATAAAGCCATGTCGATACCGGCATTAAGCTGCTGGGTTGTTTCCCATTTCACATTCGGATTGGCAAATCCCAATTGGGTAAAACCATTTGCAAGAATTTCGGAAGTTCCGGTACCAAAAGTATAGTCGTAGTTTTTGTATATAGCTGGTGCGTATGAATAATCAGGGAAGTTCTGATTACCGGTAGTACCATAGCTGAATCTTAATTTTAACGAATTTACTAGCCACCTGAATCTGTCGAAGAATGATTCCTCAGAAATATTCCATCCTACAGACAATGACGGGAACAATCCCCAACGATTTTCTTCGGAGAACTTAGATGAACCGTCGCGCCTGATACTGGCACTTGCCATGTATTTGTCTGCATAGCTATATTGTAGACGACCCAACATACCAACCATTGTACTGATACGGTCCTGTCCCCACTGGCCACTGCCTGTACCCACAGTCATATCGGATGTTCCCGCATTTAGGTTCGGAATCTCGTTAGTAACCAAATCCATTATACTGGCATAGAACATCTCGTATGTATATTTCTCCATACTGAAAACTCCGGTAAATTTAATATCATGCTTTTTTATCTTCTTATTATAATTTACCATTGTTTCCCAAGTGAGACTGGTATTCTTTGAATGAGTATCTTTTAATTGCGAACGGTAATTAGAGCTGGTTACCTTTTCGCCTTTCTGATTATATACCTCAAACTCAGGTCGAATTGAGACAGCTTTCTGATTGTTATATCCAAAGCCCAAACGTGTGGAAACATTCAGTCCGGGAATTACATTATAAGCAAGATAAAAATTACCGTTAAATGATTCTGTGTCCTTATGATTTTCCTCTTTCAATCTTCCCAATGTATAACTTACGCCCTGTAAATCTGCAGGATCGCCAGCTGCATTTACTATACTTGCCTGTGGATAAATCTGAGAACGAGTAGGCGAGTAGTCATAACATTCGTTCAATAACCCCCAAGCCGGAGATAACTGGTTTTCTATCTTCATAGCGATGTTAGTGTTGATAGTCCATTTTCCGCGCTGAAAATGTGTATTCGAACGAATATTATATCTTTTGTAATCGGAATTTATCAACACACCTTTCTGGTCGAAATAGTTCGCGGTAAGGTTATATGTCAAATCTTTCTTGCCGCCATTCGCAGTAACAGAATAATTCTGTATTGGTGCGTTATTATTGACTACATATTCATATAAACTAGAGTTGTTGAAGAAATTCACAGGATATGTTTTCAGATTAGACCAGGCCAGGTCGTCTGTATTCTGGTTTCCTTCCATCATTCTGTTAGACATCACTTTTACAAATATACTCTCGTTGGCATCAAGCAAATGAATATTCGAAGTAATGTGCTGTACACCATAATATCCGTCGACAGCTATCTTCATTTCTCCTTCCTTACCCTTCTTTGTGGTAATAAGGATAACACCGGAAGCACCGCGAGTACCATAAATGGCAGCCGAAGCAGCATCCTTAAGAATATCTATACTTGCTATTTCGCTACTACTCAATCCCGGGTCGCCCTCGAACGGGACACCATCGACAACATATAAAGGAGAACTGTCGCCTGAGATAGAACTTAAACCACGAATCTGGATGTTGGATTTGGCTCCAGGCTCACCAGAACTTGCCTGAACGTTAACTCCGGCAACCATACCCTGAAGAGCTGTACCCAAGTCGGAAGTACTGATCTTAGTAATCTCATCTGAGTTTACACGTGCCACTGCACCTGTCACCTCTTTTTTACGCATTGAGCCATAACCTACAACAACCACTTCATCCAACACTTTTGTGTCTTCCTGAAGCTTGATATTATAAATCTGACCATTCTTGATTGCAGCTTTTACAGTTTTATACCCAACAAAACTGAACACTAAGTTACCTTTAGTCGGTACCCCTTGAAGAACGAAATTACCATCCATATCAGTAATAGTTCCAAGAGAAGTACCTTCAACTTGAACAGCTGCGCCTATAACTTCAAGGTTATTGGCAGCATCAATCACCTTTCCTTTAACTGTTATCTTCTGTGAATACATAGACAATGTATAGAAGATAAGCATCACGAACAACATGTACCTGCCATGGTACCATTTTTTCTGATTTCTCATTTGTAAAAATTTTAATTTAGCAATAGGTTATGAAATTCCTTTTATAACTGACGCTAAATTATTTATTTATAATGGTACAAAAGGGGAGAATTATATATTTAAAAAGGGGGTAAAATTTTACCCCCACTTATATTAAGAATCCAAATCGGTCTGTATACTCTGTTCTTTGTACTGTTGCGGCAATACACCGAATTCTTTCTTGAAACATTCTCTGAAATACTTCAAATCATTGAACCCTACATCGTATGTCACCTCTGATACAGAATACCGTCCTGTCTTCAACAGTTCTGCCGCTCTCTTCATTCTTATTGAACGTACAAAAGCATTGGCTGTTACTCCCATAAGTGCTTTCAGCTTCTTGTTCAGAACCAAGGCCGTCACGCCAAGACCTTTACATATATCCTCTATCTGGAACGAAGAGTCTGTAATGTTGTCCTCTATTATCTTTACAAGTTTCTCAAGGAACTTATCGTCGGTAGATGTAGTGCTTACCTCGGAAATCTTTATTGCCGGAACTTTCTTGTGTTGAAGAATCCGCTTCCTGTTGGTTATAATGGAATTAAGCAGCTCTTTCATTATCTTGTTGTCGAAAGGTTTAGGGCAATAAGCATCTGCATGGAATTTATATCCGATGAAATAATCCTGCAATGTAGTCTTGGCTGAAAGCAATACTACAGGAATATGAGATGTCCTTACATCCTGCTTGATTCTCTCACACAGTTCCAGACCATTCATGCCCGGCATCATTATATCGGATAAAACAAGATCCGGTTGCAAATCTGGAATCATGTTCCATGCCATCTCCCCATCATGGGCTATCATTATCTTATACTTATCCGACAACAGTAATGACAACATATTACATATATCCTTATTGTCATCAACAATCAATATAGCCGGAGATTCTCCGTCCACTTCTATGTCTATCATCTCTTCATGCTCGCACGATTCACTTCTTAACACATCAGCAAACTTTTCATCCTCCCCACTGTTGGCAGAGATATTCTCCGTAACCATGTCCCCCTCAGTTATCATAGGAATTACAACATGGAAAACAGTGCCTTTACCTTCCTCTGATACAAACGTAATATTTCCATTATGTATCTCTACAAGCCGCTTGGTCAGAAACAGACCTATACCGGTACCTCCTTCAGCAGAGTTTTTATTCTGACTGTAGAAACGCTCGAAGAGGTGTGTTTTCAGGTTGTCGGATATTCCGTTTCCCGAGTCTGCCACAGAGATGTTTATTTTGTTATCCTGTTCATTGACAGTAAACGATACAAATCCTCCGGCAGGAGTATGCTTAATGGCATTCGATACGAGATTATAGATTATCTGTTCCATAAGATGAGGGTCGAACAGAAAGCTTATATCACTGCGTGAGACAGAATATTCCAGCCCTACACCTTTCTGTTTTGCCCAATACGTGAACTGCTGAAATACTTCTTTTGAGAAAGACGAGAAGTTGCCATATTTGAGATTCAGACTAAGCATTCCTTTCTCGCTCTTTGAGAAGTTCATCAGCTGGTTGACAAGACTTAACAGGAACTTACTGTTATGCTCCATTGTCTGCAGCATGCCGGCAAGATACTTGTCGGACGAATACTTGCCCGATTCAATAATCATACTAAGTGGAGAATGAATAAGTGTGAGTGGTGTCCTCAATTCATGCGATATGTTGGTAAAAAATGTAGTCTCCTTTTCAAGAAGTTCTTCAGTCTTGCGTTTTTCCATGTTTGCTATATATAGAGCATTTCTGCGCTGCACCCGTGAGGTATAATACACCTTGAACCGGTATAAAGACAAGACAAGCAATATAAAATAGAGTGTATAGGCATACCATGTACGCCAGAAAGGAGGGTTAATAATGACAGGTATGGAAAGTTCATTCAAACTGTAGACTCCATCGCTATTCCTGACCCTCAGTCTGAACATATATTCGCCTGAAGGAAGCTTTGTGTAGAAAGCCTCACGATGAAAAGCGGAGGTGGAAATCCATGAATCATCTACGCCTTCGAGCATATATTCGTAACCAACCTTATAAGGACTTCTGTAATCCAGGGAGCTGAACTGGAATGAGAAAGTGTTTAAATTATAAGGCAATTCAATGTGCTCTGTAAAACTTACACTTTTGTCGAAATAAGCTGAATATGTGGAATCTGCCTCAACGCTGTGATTGAAGATTTTAAAATCAACGAGTGTAGGACTACCGTTGAAATCTATCACATCAAAGTCATTAGGTCTAAAGACGTTAATTCCGTTTACGCCACCGAATATCATTGTTCCATCCGTCATTACTCCAGCAGAAAGTTCCATAAATTCATAATCCTGAAGACCATCGAAAATATCATAAGATCTTATTCTCTGTGTGTTGATATTCAACGAATTAATTCCTTTATTGGTAGAAATCCATAATGTTCCATCCGTGCCATTAACAATTGATTTTATTGTATTGCTGCTCAACCCGTCTGCAGAGCTAAAATTTTCAACGCAGGCATTATGGTTTTCATCCAAATCCACGATTTTCCTTAACCCACGTCCAAGTGTTCCATACCAGATATTATGATTCAAGTCTTCACATACAGGCACTATATAGTCGAGTTCATCAAGTCCCTTGACTGAGTTCAAAACAGGATTATCTATATACAAATCTGCAGATTCCAATACTTTAAGACCGAAGCTGGAAGCTACCCATATATTACCCTTATGATCTTTAATGATGTTTCTTACTATCTTAAGTTCTTTATTGTCAGATGTTTTGATTTCCTTCATCACACCTGTGGACAAATCATATCTGAAAAGACCTTTATTATATGTGCCAATCCACAAATATTTTCCATCGGCAAGCATTGCGCGCACATTTCTCAAACCTGAGATCTTTTTATAATCATTATCAGAAGTGAAACTGTAAATACCATCGTACATCAGAGACACATACATGCAGTCGGTGTAGTTTGAGTATGCTGTTGAGTATACTATCCTGTTTGCCGTGAAAGGAATAAGTCTGGCATTACCGGTAATGGAATTAAAATGATATAGCCCTGAGCCTTCTGTGCCTAAATATATATCAGATTTGGCAAATGTATAAACGGACGATATATGATCATTTCCTATTCCTCTGAATAAATCTATAGGTTTATTATTTTCGCGTATACTCATAAAGCCACTCTTGAAAAATCCTATCCAAAGAATATCGTTTTTATCAAGAACTACAGTTTGCGGATAGCTGTAAGAATATGTAGCAATAACCTGTGGTTTTGACTCGATGGCATGCAATACATCAAAAGTCAACACATTCACAGTGCTTGTAGTGGCATAAAATAATCTTTTGTTTTTATATACCATTTTTCGTATATCACAGTTTTCCAACAGGGTACTTACCTTGCAGGTATGCTTGTCGTATAAACATAATTGATGATTTTCCAGATTTGAGTACAATATTTGAGAAGATGAGATGACTATGGCTGAAGCTATAGGGCATCCCAATAGTTTGTTAAGCAGTAATTCATCTCCATCGACGTTACATTCGTACAGGCCGTCTTCGGAGGAGAGCATTATCGTATTATCTATTTCTATGATGTCGGAAATGTATGGTAATTTTAATGTTGATCTTAAGACAGTATTTATTTTGCCATTTTGAAAATCATAATTTACAAGGTATATACTTTCATCAGAGGAATGAAACCAGACTCTGTCTTTAGAGTCGACAAGAATCTTATCGCAAGTGAAATTTTTATCAATACCGCTGTGACCAAGATTTAATGAAACGAATTCGTTCTTTACAGAATTGAACAGGAACACTCCTCTATCGGCTGTACCTATCCACAGATTTCCATGTGAATCTTCGTCAATACATACTATCAGATTACTGTTAAGACCGTTTGACTGATATCCGTAAACCTTAAATTCATATCCGTCAAACCTGTTCAGTCCGTCGTTCGTGGCCAACCATATAAAGCCTTTTGAGTCTTGATAAATACATTGCACATCATTTTGGGAAAGTCCATCAAGAGTAGTGTACTTTCTTGTGACAAACTCATTGGATGCAAAGGATTTGCAAACTATAATCAGAACTGATATTAAACTTAAGATTAATCTAAACATATAACTATTATTCTTTATATTTCATCAAGATTACAAAGTTATTGATTTTATCTAAAACATCAAGTATTTACAGTAGTTAATAGATAATTATAGATATTTTCCACTTTAGAATGCGTATCAAAATCAATCAAGAAAAAAATAAATCTTTAACTTCATTTCATAGTATAAAACAAAAAAAGCATCGTACCATTACACTCAATAATAGATACGATGCCCGAAAGAAATTACAGTAACAGACTGTATTGGGATTGTTCTTAAAAAGACTTATCTGTATGACTTTATATATATGTCGAGTATTTCGGTATCCGACAGTTCATGAGGGTCCAGACTGAACAATGCACCCATGGCAGTTCGCGCATTATCAATCATCTTAGGGAAATCTTCCTTTACTATTCCCCAGTCGCTAAGCTTCAAATCGCGGACATTGCATTCCTTCTGCATTCTCACCAAAGCATCTATAAAATGTTCGGGATTAAGGTTCTTGCATCCGGTCATAACATCTGCCATGCGCATATATCTCTTTGTCCTGTCATAAATAAAAGTAGAGAAATAGGCCTCGCTTATAGCTATCAGGCCAACACCATGAGGAAGAGCGGGATAGTATGCGCTGAGAGCGTGCTCGAGAGAATGTTCGGAAGTACAACTGGATGTGGATTCAACCATTCCCGCCAGCGTACTTGCCCAAGCCACCTTTGCCCTCGCTTTCAGGTTATTTCCATCCTTCACCGCAACAGGTAAATATTTATACAGCAGTCTGATGGCCTCAAGAGCGAAAATATCACTTATTGGGGTTGCACAATTGGCAATATAGCCTTCGGCTGCATGAAAGAATGCGTCGAATCCCTGATAGGCAGTCAGATGTGGCGGAACTGAAACCATCAGTTCCGGGTCGATTATCGACAGACATGGGAAAGTTAAAGTGGAGCCGATACCTATCTTTTCGTTTGTTTCCAGATTGGTTATGACAGTCCATGGGTCAGCCTCGGTTCCGGTTCCGGCTGTTGTAGGAATGGCTATGATGGGCAATGCTTTGCTGTAAGGAAGCCCCTTGCCGGTACCTCCTTCAACATATTCCCAATAATCGCCATCATTACATGCCATGATTGCAATGGATTTGGCCGTATCTATCGAACTTCCGCCTCCCAAACCTATAATCATATCGCAATTTTCCTCACGACAGATTGCCGTACCTTCCATTACATGGTCTTTTATTGGGTTAGGCAATATCTTGTCGTACACCACGGCATCAACATTATTTTCTTTCAGCAGACCAATCACCTTATCCAGATAACCATATTTACGCATTGATGTTCCGGATGAAATGACTATCAAAGCCTTTTTGCCGGGCAATGTCTCTGTTGAAAGACGTTTAAGTTCGCCACATCCGAAGAGAATCTTCGTCGGAATATTATAACCAAAAACAAAATTATTGTCCATAAATATTATCAGTCAGTCAACTTACTATCTTAAAGCCTCATCAATCACTTTCTTGAGTTCAGGATAAGCCTCATCTGTATCGCCCACCTGTTTTCTCAACTCACGCAGTTTCTTTTTCATGTCCTTAAGAACTTTGGCGTATTTAGGATTATCAGCCAGGTTTACCATTTCGTAAGGGTCGTTCTTCACATCGTAGAGTTCGAAAGAAACCGGAGTAGGAACAATCTTGTGGCTGTTCTTCAACCATGACATTGATTTCTGTCCGTAACGTTTGTCGTCGTAATGACGGCCATAGAAAAGTATCAGCTTATAGTTTTCCGTGCGGATACCTATGTGTGCCGGAACGTCGTGATGAATCATGTGCATCCAGTATCTGTAGTAAACAGCATCCTTCCAGTTTTCTGGCTTTTTGCCTTCGAACACAGAGGCAAAGCTCTTTCCATCCATGTATGAAGGTTCTTTGCCACCGACCATCTCTATAAGAGTTGGAGCAAAATCAATGTTGTTAATCATCAGGTCCGACTTGGCTCCCTTGTAAGGACATCTCGGGTCGCGGACTATGAAAGGCATTCTTTGAGATTCTTCATACATCCATCTCTTATCCTGCAGATCGTGTTCGCCAAGCATCATACCCTGGTCGCCTGTATATACGATAATGGTATTTTCCCAGAGTCCTTCCTTCTTGAGATAGTCGAAAAGACGTTTCAGGTTGTCATCCACACCCTTTACGCAACGCAGATACGATTTCAGGTAATGCTGGTAGGCAAGGTATGTATTCTCCATTTCATCACCTGTATTGCACTTATATTCCATTACATAATTGCGGATTTCATGACGGCTTGAGACAGAAGTTCCGATGAAGTGACGAAGTGAATCGTTCTTGCCTCTTGTGCCTTCGGAGCCCCATTTGTCTGTATCGAACAATGACAATGGAACAGGCACTTCCACATCGTCAAGATAATATTCATAGCGCGGTGCGTACTCGAACATATCGTGCGGTGCCTTGTAATGATGCATCATGAAGAAAGGTTTGGACTTGTCGCGTCTGTTCTTCAACCAGTCAATAGCAAGGTTGGTCACGATATCCGAGGAGTAACCCATTTTCTTTATCTGGTTATTAGGCCATTTCTTGTCAGTTACGTCACTTGTAAGGAAAATAGGGTCGAAGTATTCGCCCTGTCCGCCATGACCGTTGAATACAGAATAATAGTCGAAGTGCGACGGTTCGCATCCCAAATGCCATTTACCGATCATGGCAGTCTGATATCCCATATTATGGAACTCATCAACCAGATATTCCTGGTCCGGCTGAAGCACTTCATCCAAAGTGAGCACCTTGTTACGATGGGAATACTGTCCGGTCATGATACATGCACGGCTTGGGGTACTGATGGAGTTTGTACAGAAACAGTTCTCGAAGAGCATACCGTCCCTTGCCAGTTCATCAATTGTAGGAGTAGGGTTCAGTACTGCAAGACGACTTCCGTATGCGCCGATAGCCTGCGAAGTATGGTCGTCCGACATGATGTAGATGACATTCATCTGTTTCTGCTGTGCTGCGACACCAACACATACAGACAGGAATGGCATAACAGCCATTCCCTTCATTATATTATTTTTTAAATTCGTTTTCATAAGTCAGATTATCATTGAAATAGAACTTGCAAGACATATCATCGAATGATTTTACGTCCTTATTCTGCATTTTAACCCATTGTTCTGATTTAGCCTTGACAGCGACCTGAGTTGAAACCTCATTACCGTCGACTACACTTTTAAGAGTGACATTTGCATCCTCTGCATTATGGTTTGCCACACGTACAGTGATAAGGCATCCGTTATCAACCTTATCGTATAGCGGTTTGGAAACCACCGCCCCTTTAAGCTTAATCTTGAACACATGTGCATATTCAGTAGGTTTGTTCTTAGGGAAGTTTACTACAAGACCCTCGTCAGTCATCTTATAGTCAATCTTCTCTGAGCTTCCAAGCATTTCAACCGACTCAATTTCCACGTTCTGGCAATACTTAGGAGCAAATGACTTGATAGTAACACTACCATCTGTCCAAGCCAGAGACACGGCATAGAGGTTATTGTCGCGTGTAGTAAAGCGAATGTCGTCCGCTGTATATTCAGTTTTTGTATTGTCTGTCATATAACCTGCGGTGCCTGCGTTATGTCCTTCGAAAGCAATCACCCATGGTCGTGAGCCATAAATAGCCTCACCGTTAGTCTTCAACCATTTACCTATCTCGGCAAGTACGTTCTTCTGTTCGTCTGTAATAGTACCGTCGGCCTTAGGACCTATATTCAGCAATAAGTTACCGTTCTTGCTGACAATATCAACAAAGTCGTCGATGATATGGTCAGGACTCTTGTTTTCCTCGCCCACACAATAGCTCCACGATTTCTTGCCTACAGAAGTATCAGTCTGCCATGGATATTCACGGATTCTGTCGCTCTTACCTCTTTCTATATCGAACACCTGGATATTGTCGCCATATCCGAATTTAGTGTTAACCACAACTTCTTTATTCCAATCAAGAGCCGAATTGTAATAATAAGCCATGAATTTATAGAAAGTAGGCTGGAACGGATATTTTCCCACAGTCCAGTCGAACCATATCAATTCAGGCTGATATTTGTCGATAAGCTCGTATGTATGCATAAGGAACTGACGGCGTGAACGTTCGTTCGAGCCTTCATACTTACCACAATAAGGTGTCATACCCTGACCTTCGGGCTCATGCAGTCTTTCGCCATACAGAGTGATTGTAGTGTCCTGAACATCAGAAGGAGTTTCCATTCCATATTCATAGAACCATGCATTCTCGCATCTGTGAGAAGAAAGTCCGAAACGCAGACCGGCTTTCTTGGTAGCTTCCTTCAATTCGCCGATTATATCCCTTTTCGGTCCCATATCCACAGCATTCCACTTATTGAAAGTACTGCTGTACATGGCAAATCCGTCGTGATGCTCGGCCACCGGAACAATGTATTGTGCTCCAGATGATTTTACCACTGCCAGCCACTCGTCGGCATTGAAATTTTCGGCTTTGAACATAGGGATGAAATCCTTATATCCGAATTTGGTCAAAGGACCGTAAGTCTGTACGTGATACTTATTAATAGGATGACCTTCCTTGTACATCCAGCGGGAATACCATTCACTGCCGTATGCAGGAACGGAATAAACTCCCCAGTGGATAAAGATACCGAACTTGGCATCCTTAAACCATTCAGGAATAGTGTAATTTTGAGCAATCGATGCCGAATCGGCCTTGAACACATCAGTACCTTTTAAAGATACAGTAGAATCTACATTAGGAGCGTATGTAGAATTGCACGACGCCAACAGGCTTAATGCCGCAACTCCTAAAACCGTTTTCATGGATTTCTTATTCATAATAATCTTATTACATTAAATAATGACATTAATTTTTTCTGTAAGCAAAGATACACTTGAGTTCCATTTACAATAAATAATTTAATTACTATAGTAAGGGGTAAAATATTTACCACCTATTATTGAACAAATTTACCCCCTCTCATATATGATAATAAACTGCCAATATCGAATTACAAGTAAATATATATTTCAACAAAAAAGGTTTAGCCTATTATTACACAACAATTTCACCCTAAGAATAAAATATATATAGAGTAAATTTGCCAATATAACAAACTGTAAAAACAAATTTATGAAAAACTATTTGATTTACTTACTCGCAGCAGTATCGTGTACAACTGTAGCAGACCTAAATGCTCAAGTCAGTACAAAAACAGGTAATGAAACCACAGAACTTACAATTCCGAAAAAGTTCTACAAGGACAGCATTGATTTCAGCAATGCTCCGAAAAGACTTAACAACAAGTACCCTCTTTCCGACCAGAAGAACGAAGGCGGATGGGTTCTAAACAAAAAGGCCTCTGACGAGTTCAAAGGAAAGAAGCTGAATGAGGAAAGATGGTTCCCGAACAACCCTAAATGGAAAGGAAGACAACCTACTTTCTTTGCAAAGGAGAATACTACATTTGAAGACGGCTGTTGCGTGATGAGAACTTACAAGCCAGCAGGATCACTGCCCGAAGGATATACTCACACTGCCGGTTTCCTGGTAAGCAAAGAACTTTTCCTTTACGGATATTTCGAAGCAAGACTGAGACCAAACGACTCGCCATGGGTTTTCGGTTTCTGGATGTCGAACAATGAAAGAAACTGGTGGACTGAAATAGACATTTGCGAGAACTGCCCCGGCAATCCTGCCAACAGACATGACCTGAACTCGAACGTGCATGTATTTAAAGCTCCAGCAGATAAGGGTGATATAAAGAAACATATCAACTTCCCTGCCAAATACTATATACCATTCGAATTGCAGAAAGACTTTCACGTATGGGGACTTGACTGGAGCAAGGAATATATCCGACTATATATAGACGGAGTACTGTACAGAGAAATAGAGAACAAGTACTGGCACCAGCCATTACGCATCAATCTTAACAACGAATCGAACAAATGGTTCGGAGCCTTGCCGGACGACAACAATATGGATTCTGAATATCTGATAGATTATGTAAGGGTGTGGTACAAGAAATAAGAAATAACATAATCTGAAATTATAAAAGGCAGTCTTCATTATCAGTATGCTGATGATAAAGTCTGCCTTTTTAACAAGAAGATAAAGATTTTAATCTGCCCTATCACTCATTTACTTCATCCGGATACTCTGTAAGCGAGTTTCCCGAATTGCTTATTTCAATAGAGCCGATAGGAAGATAATTGAACTTCTTGCTCCATGCAGAGATACCATAATCTCTTCTAAGAATAGGCATCATGACCTCCTCGGCACGTCCTGAGCGGACGAGGTCAAACCATCTGTCACCCTCGCATGCCAGTTCACAACGACGCTCATACCATAGAACATCAATTACGCTTTTAAATCTGTCAGGATACATCTGCATTAGCTTGTCAACATCAATATAACTTCCGTCGTCTGCATGAACATGCTTCTTTCTGAGTTCATTTATGTAATACTTCGCTTTTGCTTCATCAGGATTAGTACCTCTGAGATATGCTTCGGCAAGCATCAGATACACTTCACCATATCTGATGACCCTTACGTTTCCAGGCTTGTTTAGATTGGGGTTTCCTATCATATCGTAATTTTTGAAAGGAGGATATTTCTTCTGGGCATATCCCTGGAAATCAGGCCCGTAAGAGCCTGTCTCCCAAACAACTTTTTTTGATTCATCCTGAATATTGGCATTAGGTTTGGTTACAAGTTCATCGTAAGTAAATATCGCCGCATCACGACGCACATGGTCATCCGGAAGGAAATAATCATACAATTCCTTAGTAGGCAGACAAAAGCCATATCCATTATCATAATCAGGACTATTTTTCAACTGTCTCGGTCCGCAGAAAGTCACCCACATAGCACCTTCGCCTGCATCAATATTACCCCAGTTTGTATTACCAGATTTGGTAGAGGTCTGTATTTCAAATATAGATTCCTCGTTATTCTCCTGATGAGCCGCAAACAATTTAGAATAATCATCCGTCAGAGTATAATTACCACTTGAAATTACATCCTCCAATAAAGGTTTCGCTTTGTCAAAAATCTTAGCATCATCGTTGCTCCAGTCAGCCCAATAAAGATAGACCTTGGCCAACAGGGCTTGAGCCGCAGTCTTGGTAATACGTCCTTTCATTGTGTCCGGGAAATTATCCTTTAGAGAAGGGATAGCTTCAAGAAGATCTTTCTCTATTGCTTTATTTACATTTTCGCGAGTATCTCTCGTAAACTTGAATCCTTCAGGATAAAGAGTCTCAAGACTGATAAAGCATGGACCATAATATCTCAACAATTCAAAATGATACCAAGCACGTAAGAACTTAGCTTCAGCTTTATAAACTTTAGCTTCCGGACTGTCATACTCTGAATTTATTACAAGATTACATCTATATATACCACGGTAACGAGTTTTCCACAAATTATCGGAAATAGAATTGACACTCGTATTTGAATAATCCTCTATAGCCTGCATGTAAGGCTGATCCTGATCAGAGCCACCACCAGTACGAGCATTATCCGAACGGATTTCACCCATAGGTACAATGGAAGCAAGTGCATTACCCGAAGCACCACCTATGTGAGCTAACGGATCATAACAAGCAGTAAGCGCTTTGAACATCTGTTCATCGGTCCTATAAAAAGAACTTTCTGTTTCGGACATTATAGGAGCTGTATCCAGGAAACTGTCGCTGCAAGATGATGATGCAATAGCAGCAAACATGAGGACAAGAATATTATTATGTATTTTCGACTTCATAATTTTCAATTTTAGAAATTAAGACTTAAACCAAATCTGAATGTACGGGCCTGAGGGTAAGTACCATAGTCAATACCTGTGCTAAGAATATTGCCACCTGCCATATTTCCTACTTCAGGATCCATAAACGGATAGCTGGTGAAAGTGGCAAGATTATCAATTGCTGCATAAATTCTTGCTTTATTCAGCATCAACTTGTTTATTAATTTAGTTGGGAATGAATAGCCTACCTCAAGTGAAGAAATCTTTAAATGCGAACCATCATAAAGATAAAAATCGGATGGTTTGCCAAAGTTTCCATTAGGATCTTTGGATGAAAGACGAGGCACTCCATTATCATCACCTTCTTTCCGCCATCTGTCAAGATAGAATGATGGAAGGTTGCTGCGTCCGTATGCTTCCTGTCGGTAAATATCAGAGAAGACTTTATATCCAGCTTTTCCTGTTAAGAAGATTGTCATATCAATACCTCTCCAGTCGGCACCTAAATTCAAACCGAATGTCCATTTTGGCCAAGGATTGCCACAATCGGTTCTATCTTCATCTGTAATCTGCCCATCGTTATTTGTATCTTGCCATATAAAGTCACCCGGAACGGCATCAGGTTGTATCACTTTACCGTCTTTTGATTTATAGTTCTGTATCTGCTCTTCATTTTGGAATATTCCTAAGTTCTTATAAAGGCGGAAATAACCCATAGCATGACCTTCCTCCATACGCGTTACATTAACAGATGTTCTCCAGCTACCACCATCAGTATATCCATTTACATTTCCTATCTTTACAACCTCATTTTTAAGATATGAGGCATTTGCGGAAATAGAGAAGTTGATTTCGTTCCAATTTTTATTAAATGTCATCTGCATTTCCACACCCTGGTTTGTTATATTACCAAGGTTTCTAAAAGCTGCATTATTACCTCTAATGGCTTCAACTGTTGGCTGGAACAACAAATCCTTAGTACTTTTTTTAAACCAGTCGAAACTTGCTCTAATCATACCATTATAGAATGTCATATCGGCACCAACATTAAATTGTTCAGAAGTTTCCCATTTCACGTCTGGATTAACAAGGTTATTAGGAGCAGATCCCACAGTGATGGCATTACCAAACGTGTAATTATAATTATTGCCAATAATAGAAGTATAGGAGAATGGAGAAATTCGCTCATTTCCGTTCTGTCCCCAAGAGAATCTAAGTTTGAAGACATCAAAGTTCTTAATTTTCCAGAATTTCTCATTTGAAACATTCCAACCTAATGAAACGCCCGGGAAAGTAGCATATCTGTTATTGGGACCGAAATTTGAAGACCCATCGCGTCTGACCACAACTTCCGCCATATATTTTTCAGCATAATTATAGCTTAGACGAGCAAAATATGAGAACATACTATGTCTAGGATTAGCACCGCCACTATTAGCTGATGTCATAACATCACCAGCATTAAGATACCAGTAATTCTCATTGGTCATTGCTTCATTTGGATATTTATTTCGTGTTCCGGCCATAAACTCATAAACATCTCTTGATGCAGAAGTACCTAACAGGACAGATGTAGAATGTTCACCAAAAGATTTTTTATATCGCAATGTATTCTCCCACTGCCAACTACTATTAGCATTTGTACTTTGTTCTACCCTAGAATTATCTTCTTTACATTCTGCAGAATGAAAAAACTTTGGTGCAAACATTCTTCCACGGAAATTCCGATGATTAATACCAAAATCTGTGCGGAAAACAAGGTCTTTAATAAAAGTGATCTCAGCATAAACATTACCAAAAAATTGCTGGGTAATATTTTTATTCTTAGGTGCCTCATCCATAAATGCAATAGGGTTCCACATACGGCTATAAGGTACAGGAGAGACTCCATATCCGAAAGTATCGTTGCTATTCTCATCATAAACCGGAGTAGTAGGATCAATATTATAGGCGTATGATATCGGATTATAACCATTGATACCGGTTGCCACTCCACTATTCTCTATATATGCATAGTTGACGTTTGCACCTACACTTAAGAAATCATTTATAGAATAGGAACTGTTCAGCCTTGTGCTGAATCGTTTGTAAAATGACGCATCTTCACCGATAATACCATTCTGGTCTAGATAATTCAATGAAAGCAAGCTTGAACCCTTATCACTGCCAAAGTTAGCAGTAATGTTATGCTCAGTAACAGGAGCTGTATTCAATATTTCATTAAACCAGTCTGTATTATAACCTGTTGGAGCAGTAGGTACACCACCGGCAAGCGGCATATCATCATTGTCGGCAAACTCTTTCATCAGCATAATGTACTGTTCATCATTCAGCATGGTTGGTTTCTTTGCTACTGTAGAGAAACCATAGTAACCATCATAAGCAAGCGATGTCTTTCCTTTCTTTCCTTTCTTTGTGGTTATAAGGACTACACCATTAGCGGCTCTGGCACCATAAATAGCAGCTGAAGTTGCATCCTTCAAGACTTCCATGCTTTCAATGTCGTTGGGATTTACACTGTTCATGTCGTCCATAGGCAGTCCGTCAATTACAAAAAGAGGATTAGAGTTTCCATTTGTACCAACACCACGAATTACCAGCTTCGGTGCTGTTCCTGGCTGACCGGAATTTGTCACAACGTTCACACCACTAACCCTACCGCTCAATGCATTCACGGCATTTGCTGGTTTAGATTGCAATAAATCATCGGAATCGATGCTACTGATAGCACCTGTTACAACACTTTTTTTCTTAACCTCATATCCTATTGCTACAACTTCCTCGAGTGCAATGGCAGATGTTTTTAATTGAACGTCTATCTTAGACTGACCTTTATACACTATATTCTGTGTATCATATCCTACGAAGCTATAAATCAATGTCGATTCCATTGGTACATTTTCCAAGATATAATTTCCGTCCAAATCAGAAATAATACCGTTTGTGGTACCTTTAACTAAAATACTTGCACCTATCACAGGTAAACCATCGGAGTCTGTTATACAACCGGTAACTTTCCCGTTCTGTGCATTTAATGGTAAACTGAACGTTATAAGAATCAGCATACACATTAATGATAGTGTTCTGTTCATAATCTAGAGTTTTTTGTAATTAGTGTTTTTCTTAAAATAAAAAGTTTTGTTCTATCAGTTGCGCGCTACTTACTGACACTTGCAAATATATATACTATGTAATATAACCAAAGGGGGAAAATTTCATTTAAATAGGGGGGGGAAATAGATTAACTAAATATTTTAAGGAAAAATGGCTGTTAGAATCCATTCCCAGACTCCAACAGCCATTTTATCACTAACAATCGCCTGTTAATCAATATATTTTTCTGCCCATTTCCTTAAGATTTGCATCCCTGCCCAGTGGAACAAAAGTAAATCCGTATGAATAGCTTCCCTTCAGAAGACGCTTGTCTATTGAAGGACGGGCTTTCAGACTCCAGCTATCTGTTCCGCCCACTCCAGCCTGAACCAGGTCGATATTAAGAGTATTAGAATACAAGTCCTTTTCAAGTTCATTTATATGTTTAGCCTTATCAATCGCATTCTGCGACATCTCCCACACTGAAACAGATAGGGGTTCATCGCCGACAATCATCACACCTGCCTTATCCGACTGCAAGGCAAACCATCTCACGTCACAACGGTTTCCGTTTTCCTGCGGCATTACATAGTCAAATCCCAGAGCGGACACCTTGCAGTTATATATAGACACCATTGCAGAGGCTTTTCTGTCGGAATAGTTTTCCCATGGGCCACGTCCATAATATGTCACATCCGACAAACGATTGGTACATTCGCATTGCAATCCTACGCGCAACATTTCTGATATTTCAGGAGACTTCATCATTGAATAATGAACGCCTATTGTTCCGTCTGCTTTTACTTTATAATTCAAGGTAAGTCTCAGTCTTTCATCTATAGCCTTTAGCACCTTAACCTCAAGATTGCCTTCCGATTTGCGTACATCTATAGAAACTGTCTTTAGCTTTAATGGAGCATCTTTCCAGAATGCAAACAGTCTATCGACCTTCCATCCTCGCCAGTCATTGTCTGTTGACGCTCTCCAGAAGTTTGGTTTCAGAGCAGATGTGATGATACTTTCATTATCTATCTTATACTGACTGATATAACCATCACTGATATTCAGATAAAAGTTCTTTCCCTTCACGCTGATGTCTTTCTTGTTATCTGAATCGATTTCCATATCCAATGTAGTATCAACGCATTCTACTATCTTTGGTAAAGAAAGATACTTAAACTGTTCCCAGGCAACCTCGTATCCAGCTTTGGCATACAGATTGTCATTCTTGAGCCTGGCACTCAGGAATAACCAATATTCCGCACCGTCATCGGCCTTGAAATTCTGAATAGGAAGTTTTAGTTTACAGCTCTCACCAGCTGGTGTTGTCGGCACAATAATCTCACCTTCCTGCAATACACTGTCTTCGTCCTTCAATTGCCAAAAATAACGATACTCATCTGTTGAAAGGAAGAAGTTTCTGTTTTTTACAGTTATCTCTCCACTATAGACATTATCAGTTGTAAATGATACAGGAGCAAACACGTACTTGCATTCCTCAGTAGCAGGTTTAATGGAGCGGTCGGCACTGATAACACCATTTATACAGAAGTTTTGGTCGTTGTGCTCCCCTTTCTCATAGTCACCACCATAATTCCATGATTTCTTATTATATTTCCGTTCATTATCCAGCAATCCCTGGTCTATCCAGTCCCAAATATATCCGCCGGCAAGCGCATCATGAGAACGTATTGCATCCCAGTATTCTTTCAGCCCGCCGGTAGAGTTTCCCATAGAATGTGCATATTCACACATTATTATCGGACGGTTCATGACCGGATTCTTAGTCATTGCTATAAGCTCATCGACCATAGGATACATACGGCTAATGACATCGACGTATAAAGGATCATCGGGATTGGCATACACACAAAGCTCTTTCTTTGCCGGTTTGACATCTTCGTTCACATTAAAATCTATCTCACTAGTAACGATTGACGCTTCCTTACGTCCGATAGGTTTGTATAAAGGATTTTCCGGCTGTCCTTGCGCCCCCTCGTAATGAACAGGACGGGTTGGGTCATAATCTTTCAGCCATCCTGACAGAGCTGCATGATTAGGGCCGCATCCAGACTCGTTGCCCAACGACCACATAAACACAGAAGGATGGTTCCTGTCTCTCACAGCCATTCTTACCACTCTCTCCATGAACGAGTTAGCCCACTCAGGCCTATTGGACAGATACCCCCTTTGATGATGAGTTTCAAGATTAGCCTCATCCATTACGTATATACCATACTTATCGCACAGTTCATAGAAATAAGGGTCGTTAGGATAGTGCGATGTACGGACTGTATTGAAGTTATAACGCTTCATAAGCAGAACGTCTTCGAGCATCTCATCACGTGTAACGGTCTTACCTCCGGTCTCGCTATGGTCATGGCGGTTTACACCAATGAGTTTAATAGGAGTGTCATTCACCAGAATCTGATTACCTGTTATTTTAATATCCCTGAACCCTACCTTATTACTTCTCGCATCCACCACGTTGCCCTTTTTGTCTGTGAGCTTTATAACCAAAGTGTATAGATAAGGGTGTTCCGAATTCCATAGTTTTGGCTTAGAAACAATTCCCTCCATCATTCCGTAATAAACATTATCACGCTGAGGATAAGGTTCGTTCACCACATAATCGGCAGTAACGGTAATGTCTTTTCCAAACACCGGTTTCCCATCGGCATCATATAATTGGGCTGACAGATTCCATCCCTTCAAATCATCCATATTCTGATTTGTTATTTCCGGACGGATCTGTAACCGTGCTATATTCTTCCGGAAATCGATGCGTGTCCTTACTCCATAATCATATATTGCCACCTGCGGAATGGACATGATATATACTTCACGATGGATACCAGCCATTCGCCAGTGGTCGGCATCTTCCATATAACTTCCGTCGGTCCACTTATACACTTGCACCGCCAGTTTATTCTCCCCCTTCTTAACGTATTCGGTAATATCAAATTCAGTAGGCAGACAACTGTCTTCGGAATATCCCACCTTCTGTCCGTTTATCCATACATTAAATCCCGAATAGACGCCTCCGAAATGGAGTATAATCCTGTCGCTCTTCCACTTGTCAGGAACAACAAACTCCTTGATATAACACCCCGTCTGATTATTCCTGTCAATATATGGCGGACGAGCAGGGAAAGGATAAATAGTATTTGTATATATAGGATAGCCATATCCCTGCATCTCCCAACATGAAGGAACAGGAATAGTTTTCCATGATGATGAATTGTACTCCACTTTATAAAAACCGGCGGGAGCCAATGCCATATCCTCGGAAAAGTTAAACTTCCATTGGCCGTTCAACGACATATACTCCGATTTCTCTCTGTCTCCATCCAAAGCCCAATCCACTCTCCGGAAAGAATAAGTAGTACTGCGGGAAGGCAAACGGTTAATTCCGTTTATGGTCTGATCCTGCCATACATTCTGATTGTTTCTCCACTGATTGGCACCGTTGTCCGATGCAGACAGAAATTGCATCATGAAAAATAACACAGAAAATGAAAAAATAGATTTTAAGTTCAAGTTCATAAATTCGCATTTTAAGTTTCTATGCAAATATATAAGTATAACGAACAATGAATAGGGGGTATTTCTATCTATATAGAGTGGTATTTTTACATATGAGCTAAAACTTAAAAAAAACTGTCAGTATTACTATGCTATGTAGCACTCTATATGAAAATATTATATATTCCCAAGTCAAAAGCCTTTTCAAACAATTTTTATATATTCTCATCCTATCCCTTCCATCAAAGATAAATTCCAATCCTGATTTGCCAGCCGCATTTATTCCTTTTTTCAGGAGAATTTTCTTTATGGCTATCGCCATGAAAATTCACCTGAAAAAGAATGCGGCGGCAAACGGATTAGAATTAAAGAAAAGATTACAGGGATTAACTGCGACCGACGTGACGCATAGCCGTAATTCAAAGGCGGCTATCCTTATATTCCATATATGACCTCACAAATACTGTGAAAATCCACTTTCCCCAATAACAAAACATAGCCTGCCATATCAACACCCAAAATAAGACAGGGATTTCAACTCCCTCCGATCTGCATAGTCTGGTGGCTTCGCTATGCTTTTACTCCTACATCCATTTTTTTTCTTTCTTTTTTCCTCTGTTCCCGTTCTTTCCTATCCTTCGTGTGACATTTGATGACACCTGATGACATCTAATGTCATCTATTTGTAAATCAATTGTTTACTCAATTTATCATCTTACATTTGGACTGTGAAACAAATCAAGTAGTCACTCAAAACAAAAGATTATGGCACAAGAAAACAGTCCTGACAAGGAAAAAAGGCAAGGCCGGACAAAGAAACCCGAAAAGCCTTATGTGGAACAAATTGACGAGCTTCTGCTGGTACATAACAAGAATGACCCAAAGGAAGGTTTGGGAGTAATCAGCAAGATGGACGAGAAAGGCAATTATCAGACGGTTACACCGGAAGAGAAGAATGAGAACTCATTCCTGAAATTCGACAAGAATTCGAGTATTCTCGAAAACTTCATCAAGAATTTCTGGAGCCAGCTGAAGGAGCCTACGCATTTCAGGCTTATCCGTATGACCTTCAATGATTACAAACAGAACAAACAGGCTCTCAAGGACCTGGCCGAAGGCAAGAAGACAGACGCGGTAAAGGAGTTTCTGAAACGCTATGAAATCAGACCGAAAGTAAACAATCAGAAAAACAGTCAAACAAAAGAGGAGGAAACAACAATGGCAAAGAAGCAGGAACAGACAACGCAGGCTCAGCCTGAACAGGTATCACAGGTGGAAGCTGCCGCACAGGGGCGCGAACAGCAGGAACCGCAACGCCAGCAGACACCCACGTACCGCTACAACGAGAACATGATTAATTGGGAGGAACTGGGTAAGTTCGGTATATCCAAAGAAATGCTGGAGCAGTCCGGACAGCTTGACAGCATGTTGAAAGGATACAAGACCAACAGAACCATGCCGCTGACACTCAACATTCCTGGGGTACTGACCGCAAAACTTGATGCACGCCTTTCGTTCATATCCAACGGCGGGCAGGTCATGCTGGGCATCCACGGTATCAGAAAGGAACCTGAACTGGACCGTCCTTATTTCGGACATATCTTCACGGAAGAGGACAAGAAAAACCTGCGTGAAAGTGGAAACATGGGACGCGTGGCTGACCTTAACCTGCGTGGCAACACGACAGAGCCGTGTCTGATTTCCATCGACAAGAATACCAACGAACTGGTAGCCGTACGGCAGGAGCATGTCTATATCCCGAATGAAATCAAAGGGATAACCTTGACTCCGGACGAAATCCAGAAACTGAAAAACGGAGAACAGATATTCGTAGAGGGAATGAAGTCCAATCAAGGTAAAGAGTTTAATGCCAATCTGCAATATAGTGCGGAAAGAAGAGGCATCGAATTTATCTTCCCGAAAGACCAGGCTTTCAACCAGCAGACGCTTGGCGGTGTACCGCTTTCCCCCATGCAGCTCAAAGCGTTGAACGAAGGACACACCATCCTTGTAGAGGATATGAAACGAAAGAACGGCGAACTGTTTTCTTCCTTTGTTACCATGGACAAGGTTACAGGCGGGCTCCAATATACGCGCCACAATCCGGAAACGGGAGAAATCTACATACCAAAGGAAATCTGTTCGGTACAGCTCACACCGGAGGACAAGGAAGCGTTACGCAAAGGGCAGCCCATCTATCTTGAGAACATGATCAACCGTAAAGGTGAGGAATTCTCGTCATTCGTCAAGCTGGACCTGGCAAGCGGAAGACCACAGTATTCCAGAACTCCGGACGGTTTCAACGAACGACAGGCACCAGCCATCCCGGCTGAGGTTTACGGACACCTGCTTTCGGCACAGGAAAGAGCTAATCTTCAGGACGGAAAGGCTATCCTCGTAACGGGTATGAAAGGTCCCAACGGCAAACCGTTCGATTCCTATCTGAAAGTAAACGCAAACACCGGACAGCTGCAATATTTCCAGGAAAATCCGGATGTGCGCCGCAATACTTCACAGCGTGCTTCACAGACTGACAATACCCAGCAGCAGGAACAGAAGAAGGGAGCAAAACAGGCTGTCTGACCTGAACGGGATTCAAATCATTCAAATCATCAATTACTAAAAAAGGAAAGAACATGAACAAGACCAATCATCATATCTACAAGACTGAACAAATCGACTGGGAGAAACTGGAATCGGTAGGTATCAGCAGATCGCAAATTGAAAAGGACGGAAACATGGACCTGCTCCTTCAGGGAGAGGAAACCAATGTCATGTCCATTAAAATCAAGACTCCTGTATTTTCACTGACCATGGACGCCACACTCAGTCTGATTGAAGACGAGAATGGAAATCCGGTCATCAGCGTAAACGGTATCAACCCTTCAGGTGAATAAATAAGAAACCATAATGTATCATCTCTCTTTCCATACGGACTTACCGTATGGAAAGAGATAAAAACAGAATTTATCATGATTGCCATATTAACAGACAAACCAAGTGTAGGAAAAGAAATCGGAAGAATCATCGGTGCAACCAAAGTAAGAAACGGATATGTGGAAGGAAACGGCTACATGGTTACATGGACTTTCGGGAACATGCTGTCACTGGCCATGCCGAAGGACTACGGAACCCAGAAGCTGGAACGGAATGACTTTCCTTTCATCCCGTCCGAATTCGAACTGATGGTACGGCATACACGCACCGAGAACGGATGGATACCGGACATTGATGCCGTGCTCCAGCTTAAAGTAATCGAGAGAGTGTTTCAGGCATGCGATACCATCATTGCGGCTACCGATGCCAGCCGTGACGGGGAAATGACATTCCGCTATGTCTATCAATACCTGAACTGTACACTGCCTTGCTTCCGTCTGTGGATTTCCTCTCTTACCGACGAGTCTGTGCGTAAAGGCATGGAAAACCTGAAGCCGGACAGTTGCTACGACAGCCTGTTCCTTGCTGCCGACAGCCGCAACAAGGCGGACTGGATTCTCGGAATCAACGCCAGCTATGCCATGTGCAAGGCGACGGGCCTTGGCAACAATTCTCTCGGACGGGTACAGACACCGGTACTGGCTACCATCAGCAGACGCTACCGTGAAAGGGAGAACCATATTTCATCGGACAGCTGGCCCATCTACATCAGCCTGCAAAAGGACGGCATCCTTTTCAAGATGCGCCGCACACAGGATCTTCCCGACAAAGAATCCGCTACAATGTTTTTCCAGGACTGCAAGCTGGCACATCAGGCACAGATTACAGGTATCAGCCACAGCGTTAAGGAAATACTTCCACCGGACCTGCTTGACCTGACACAACTTCAGAAGGAAGCGAACATCCGCTATGGTTTTACCGCATCAGAGGTGTATGACATCGCCCAGTCTCTTTATGAAAAGAAACTGATTTCCTATCCGCGGACTTCCAGCCGTTATCTGACGGAGGATGTGTTTGACTCGCTTCCACCAATCATGGCGCGTCTGCTTTCATGGGAGCTGTTCCCTGCAGCTAAAGGAACTGGAGGTATTGACATATCCAATTTGTCCCGCCACGTAATAAGCGCAGAAAAAGCCAATGTACATCATGCCATCATCATTACAGGTATCCGTCCCGGAAATCTGTCCGAAAAGGAAATACAGGTTTACAGACTTGTAGCCGGAAGGATGCTTGAAACATTCATGGCTCCATGCCGCATAGAAACGACAAATGTTGAAGCGGTTTGTGCGGCACAGCATTTCAAGGCCGAACAAACAAGAATCATTGAAGCCGGCTGGCATGATGTGTTTATGCGTTCCGACATGGTTCCAAAATCAGGATATTCTGTCAATGAACTCCCCGAAGTGGAGAAAAGTGATACTCTGAATGTATGCGGATGCAACATGGTACACAAGAAACAGCTGCCGGTAAATCCGTTCACGGATGCAGAACTGGTGGAATACATGGAACAGAACGGACTGGGTACAGTATCCTCACGTACCAATATCATCCGTACACTGGTTAACCGTAAGTATATCCGTTATTCAGGGAAATATATCGTTCCGACCCCGAAAGGCATGTTCACCTACGAAACCATCCGTGGAAAGAAAATTGCGGATACTTCACTCACCGCAGACTGGGAAAAACAGCTGGCCGGACTTGAAAGCGGAATGATAACCGGACAGGACTTCCTGAACAGGATCAGGACTCTCGCCAAGGAAATGACTGATGACATTTTCAACACCTATTCCACAAAAGAAGAATAACATCTATACCTAATCAACCAAGAGAATGCAGGCCGGAAGGTCTGCATTTTTTTGTATCCGTACAGAAAAGAATCTGTTTTTCCGCTTTTAAGCGGCAAAGGTCTTGGATTGCCTGCCTTTTGCCGCAAGGCTGCCCTCATGGGCTTGGCTGGACAGGAAAAAATCATCCTCGCTGCGCTCCGGTATTTTTTCCTGCCAGGCCTTGCGCAAAAAGGCAATCCAAGAGGCCGGAGGCCTATAAAATCGGGAAAACACATCCCGATGGGATTATTCATTCATAAAATTAAGGATTATGAAACTACAGATTATCAGAAAGATCGGCAGACATGCAACAGCGATATTCCTGATTACCGGAATATGTCTGCTGACAAGTAAAGGGATTGTCCCTACTGGGATGATTACGCTGCTGTTGCTTGCAGGAGGGTTCATCGGTTTTCTGTTCAGGATACTGGTCATTATTTTCAAGATTCTTATTCTTCTGTTCATTGTAGGATTATTTGTCGCATAACCCAAAATATAAATATACATATATGGAAACAGTTGCTATAACCTCACAAGCTCCTGTCATGCCGGCTGTATGGCCACAGAACGAACATATCAGACCGGTTAAAAGACGTCTGCCCAATACAGTTGATGAACCTAAAAATATCGGCTACTATCTGGAATCGCTACGTGATATTTCCAGCAATCCGGACAGAGAGAATATTCTGAAAGAATTCTTCAAGGAAACTTATGTATAACCATAAAATTTTTCAATTATGTTTTTTCAATCAATTTATCAGATGATTACAGCAGGTACGGATCTGAATATCAATATCCGTAAAGTGGACAACAGCCTGAGCGTAGCAGTCATGCCAAGGCGGAACAGCCTGAAAGAGGATACGCGACAGAACATGGTGCCACTGATCGTGAACGGAACACCGGCAGAACTGGATATGGGCTTCCTGCAGACCATACTCCAACCGATACAGAAGGTACAGGGACTGCTTGTCAATGCGGAAAATTTCGAGAAACAGGCAGAAAAGGCTACATCACAGGCCAAATCATCCAAGGCTCCAACAATACCGGCCGAATCAAAGGAAGCCAGGGAAAAACGGGAAAAGATGGAAAAGCTCCTCAAGAAGGCTGATGAAGCAACCGCCGCAAAAAGGTACTCCGAAGCAATGACATGGCTGAAACAGGCACGGGTACTGGCTCCTACAGAAAAACAGAAGGATATTGACGAAAAGATGCAGGAAGTACAGAAACAGGCTAGTGCAGGAAGCCTGTTCGGTATGGCAGAGGAACCGGCGCCGGTAATTCCCCAACCACAAGGCTATATGAACGGTCAGTCACAACCAGGTATGCAAACAAGCATATTCCCGGAGCAACAGACCCATACTATGAATCCTGAACCTGTCATGCAGCCTGCTCCACAGCAGGTATCACAACAAATTCCACAAGGAATACCTCAACCGGCATATGGAACGAACGGGACATATAACCCACCTGCTCCAAACAGCCCGATAGTAAAAGGAGCAGACATACCGCAAGGCGCAACAATGCATCCTTACCCACAGCAGCCATACTACCAGCAAGAGGCGACTCCTTATCCAACACAACAGCCACAGCAACCGACAAACGGACATATACCGAATGGGGCTGCGCAAGTACAGAATGGAAACGGACGGGAATACCAGACTGCATCGGCTACACATGAGACATTCTGCTTCGATCCGGAAGACGAGAATGACAGGGAACTTCTAAGAGAGGACCCGTATGCGGAATATCCGGATTTTCCGGCTGAGTACCGAATGAAGGACGAGGCACAGGTAGAAATGGTATACTGCTGATATACACAATAAACGATTTGTAAAACCAATAAACTATAAACAATATGGCACTGGAAATTAAAGGAATGAAAAGAGTATTCAAGATGAAGAAGAACAATCAGGAAATCGTACTGGATGATCCGAACGTAAACATGTCTCCGGCTGAAGTGATGGACTTCTATTCCATGAATTATCCGGAACTGACAACCGCGACCGTACACGGACCGGAAATCGAAGACGACCGGGCGGTATATGAATTCAAGACCACTATCGGAGTAAAAGGGTAAGAGCATGAAAAAAGGACAACGTAAAGACAAGAAACCATGTACACAACTTACGGAACGGGCTTTGGAAAATTTAGCCAGACTTATCATATCGGAACTCGAAAATACGGACATAAGCCGGGGCATCAGGAACAGAAAGAAAAGAAGACTCCCTCCCGCAGAAAGCCTCATGGTTTTCTGAACACGAGAATACCTTCCATCGCTCCCGATCTGTATGTTGAGAATGACAGGGATGTAACGGTAAATGTCACCACCAAAGAGAATCTTGATTTCCTGTACCGTTCAGCCATGAAGTATGCGCAGCTCCTGGATGTGGAGCTGCCATACCATCCTACAGGCAGGACTTCCACAAGAGAGAAAATATGCCTGCTATATAATGCACTGGATTCCATAGTATCTCATCATGTAAATCTGGAACTTATTGGTGACAGGCTCCAGTTCTGCATCTACCATTTCCATGAATGGCCGGATTATACGCTTTTCTTTATGCCGATAGACTTTACGGAAAGGCTGCACGGTGAAATTAAAAAGATTACACTGGAGTTCATCAGAAAGTTCATCAAATATCACAGGATGATGGATATAACCGATACCCCTTATTTTGAGATGTCGGAAGTCTGTATCGATTATGTGGACTTTGAACAGCTCGATGAGGAAGAGAAAAAGGATTTGTACAGAAAGGAAAAGCTTTTCAGGTCATATGAGAAAGGGAGAATCCACAGGAAGCTGTGCCGGATGCACTCCAGGGCTTTCTGTAGGAATCTGGAAGAACATATCCGCAACTGTACTCCTTCCAGCGATAAGGAAAGAAGACTTTTGGAACTGATTACCGAAGGGCTGTCCCTGATTGCAAAGGACAGCCCTTATATCTTGAATTATGATTATGATTTTGCAAGCGAAAAGGAACGGGATTTCGAGCCGCCACCGCTCGAATATCAGATTCTGCTTACATATTCCATCACGGATACGGTTACCAAAGACATGGAAAGCTGTTTCAGTACTGACTGTCAGGAAACATATAACCAGACTCCCGTATCATTTACCTTCATCACGCCGGAAACAGAGGAACTTTTCAAGCCGGACAACTATCCGGAACGGTTTGAGAAATGGTTTGAGAAATTTGTAGAACATGTTACCTATAATTTATAAACATCATGAATGAACTGACCAAAAATATGCAAAAAATGATGGTACCGAAGGCTGCAATCATAGCCTACAAGTATGAAGACAGAAGAAATCTTGATACCAGGTACTTTATAGAATTACGTCCAATCAGAAAAAGCGGACAGATGGGGGCAGGTATCCCCGTCACATACGAATTCATGAATACCCTGCTGGAATCCTATACGGAAGAAATGAGCGGGATACCGGCAGGCAGAGTCCCTGAAAACATGCTGGCCTGCAATCCGAGAAAAGGACAGGAAGAATATATCTGGTACAATCCGCCCGGAAAAAGACAGATGTTCTTTCACAAGGATCTCAATATACAGGACGGCATGTTCAATCTGCCGGGAATTATCTACCAAGTAAAAAACGGAAACATGGACGTGTTCGCTTTCAAGGGGAAACGTCCGGTGGAGACGACTCCGCTGTTCCGTGCCCCGTTCTTCAACGTGACCGGATCAAGTGTCTGCCTTGGCAACAGTTCTCTGGAAAAGCCACAGAACCCGACTTTCCTTTCCCTGCTGGAATACTGGGAAAAACGGTTCTGGCTGACTGAATTCTCCCATCTGGGAGGAAATGTCAATCCTACCGTTTCAAATCTTGTCATCGTCACCGAAAATATAAGAAACAATCCGTTCGACATGAACGAACTCAAGCCCATGAATAAAAAACTTAAAGACATACTTCCATGAAAAAGATACATTTTACCGACCGCTACCTGCTCAATCCACGTCATCCGGTAACGGTATTCGTCATCGGAGCTGGAGGTACCGGCTCACAAGTGATAACCAATCTGGCACGCATGAGCATGGCACTTCAGGCATTAGGTCATCCGGGACTGCATGTCACCGTATTCGATCCCGATACGGTTAGCCAGGCCAATATAGGACGCCAGCTTTTCAGTGAGACGGAACTGGGACTGAACAAGGCCGTATCACTTGTCACACGCATCAACCGTTTCTTCGGATACGCATGGACTGCCGAACCGAAATGTTTCCCAACGAAGAAATTTTCAGGATATGATACAGCCAACATATTTATCACCTGCACTGACAATATACGTTCACGTCTTGAGATTTGGAAATTTCTAAAGAAAACTCGTAAAGAGAACTTCAATGACTATTTGGTTCCTATATATTGGATGGATTTTGGGAACAGCCAGACAAAGGGACAGGTCATCATCGGGACGGTACGTGAGAAAGTTCTCCAACCTTCTTCACAAGAATATATTCCCATGCCTAAAATGAATGTCATCACCGAGGAAGTGGACTATGCGAAAATCAAGGAAAAAGAATCAGGACCAAGCTGTTCTCTGGCGGAAGCCCTGGAAAAACAGGATTTGTTCATTAACTCCACACTGGCACATATCGGATGTGACATATTATGGAGAATGTTCAAGGAAGGAAAGACACTGTATCGCGGTGCCTATGTCAATCTGGATACATTGAAAATGACCGCAATCCCGGTGTAATGACAGAAGTGACCGTATCATCTTTCCATCAGAATACGGTCACTTATTCTATTTGCTACTTATTATTTACTACGTTCTTACCACGCTGGAGCAGGAAACTCTGTATCTCTGAGGCGAGATAGAATGATTTCCCGTTCTTTTCCACCGAGTAATATTTAATCTTGCCCTCTTGCCTGTAACGTGCCAAAGTTCTTTGTGACACACCAAGGAGTTCTGCCAGATCCACATTATCAAGCAGTCTGTCTCCATTCATACATTCTTTCAGACGATTCATCTGGTCCAGTTTCTTTTCAATGCGGGCAAATCCCTCTACCATTGTTCCTATAAGTCTTTCGAGTATCTCATTATCTATATATGACATAATTCCAATGTTATTAAGTGAATAAATCGATACTCTCTTCGTGCGCACTCTAAGAGTATGTACTTATAGTAGTGAAAATAGTATGCCTGAATCTAAGACAAAGATCAACAAGCTTATTAGGCGCTGATAATCAGGCGTATAATTTTTTCTACTTAATATTTAGTGTAAACCAAAAGTGTAAACTATGTAATACAGAATTGGGAACGGGTTAACACAGCCACCAACAATGACATCTGATGCTACCTGACGACACCTAATGACAACATTTTGTATCATATACATATTCAAAATACATTTGTACAAACTCAACTTTTTTGGATATGGAAATCATTGGAATTGAAACAGCTACATATGAAAAGACATTAAAGGAAATTGAAAACTTCCTTGATACCATTGATAAATTGATTACAGCTTCTTCACAGAAAACAATAGGGGAATGGTTGGATAACCAAGAAGTTTGCCTGATCCTCAAAATTTCTCCAAGAACATTACAGAATCTTAGAGATACAGACCAAATCTCTTATTCTCAAATTGGGAAAAAGATTTATTATAAAAAAGAAGATATTCAGAAGTTCATTGAAAAACACAACAGAAAATTATGAGCAAGGTAATTACCCAAGATAATGAGCAAGTTATTCAGATATACAATAGGTTAAAAGATACGCTAACAAGACTCGAAGATATTCTGAAGAATAACAACCCAACACTTAATGGGCATAGATATATGAATGATGCAGAATTGGCTAATTACCTTAAAGTATCAAGACGCACTTTACAAGAATATAGAAATAATGGAATCTTATCTTATTATCAGATTGGAGGTAAAATTCTATATCGGGAATCTGATATAGAAGAACTTCTTGAGAAAAACAGACAGGAAGCATTCCGTTAAACATTTCTTGGAATTTTCGTTGATTTTCAAAGCAAAAATCAGTATCTTTGCAATACTGACAAAGAGTTGTATATCAGTGCAGAACAAAGAAGTTCAATCGAGGTGAAATAGGTGGACTAAATGACAAACAACAAGATAAGTAATTGATTATTAGCGATAAAAAATATAAGGTTCCGCCCCCAGGCGGATCACTGAAAACAAAAGAGAAAT
SEQ ID NO:73(具有紫菜多糖PUL的pWD035质粒)：
aacaaatactttcaggacgatgtaaagtcctgagaaatcaaactgaatggatagataaatattagtcttttctcatcggattcttttaatatcgctactcttttattatgtagtggttgtattatcttaaataaaacaaaaacacccgactttacattcttgggtgtaaaacagggtgtttgttttgcaatctgctgattttcagcgttttttgcggagagacaggctctcgaacttatactttcacagaatatcataaaactgcaaacgactgataatcaagtgtaaattacaatatagagtaaaagtaaatgtttctaaatgtcttttgctatctcaataattgggtgtatatttgggtgtagaatttcaaacgcacccaattatgaatatcaagcgcaacatcatttttgcattggagagccggaaaaagaacggtgtgccaatcgtagagaacgtacccatccgtatgcgtgtcatctttgccagccaacgtatcgagtttacaacgggctaccggattgacgtagccaaatgggatgcagataagcagcgggtaaagaacggatgtaccaacaagctaaagcaaagtgcagccgaaatcaatacggacttgctgaaatactatgccgaaatccagaatattttcaaggaatttgaggtgcaggaggtcatgccaacgacccaacagttgaaggaagctttcaacatgagaatgaaagataccagtgaagaacagccggaagaagcccctgtcagcttttgggaggtgttcgatgagtttgtaaaagagtgcggtaaccagaataactggacggcatccacctatgaaaaatttgcagcagtgaggaaccacctcaaagagttcaaggaggatgcaacgttcaactatttcaacgagtttggattgaacgaatacgtcaacttcctgcgtgacaccaaggatatgagaaacagcaccatcggcaagcaaatgggattcctcaaatggttcctgcgctggagcttcaagaaaggacatcatcagaacattgcatacgatacgttcaaaccgaaactgaaaaccacctcgaaaaaagtaatcttcctgacttgggatgaactgaacaagctgaaagactaccagatacccaaggataagcaatacctggaacgtgtgcgtgatgttttcctgttctgctgctttacgagtttgcggtattcggatgttcgcaatctgaaaagaagcgatgtgaagtccgaccacatcgaaataaccacagtcaagactgccgacagcctgacgattgaactgaacaaatacagcaaagccatactggacaaatacaaggacatccatttcgagaattacatggctctgcccgtcatcagcaaccagaagatgaacgattacctgaaagagctgggcgaactggcagaaatcaacgagcctgtacgggaaacctactacaagggaaatgaacgtattgatgaagtcacacccaaatacgctttgctcagtacccatgcaggaaaaaggacattcatctgcaatgcgctggctctcggaatcccggcacaggtggtcatgaaatggacgggacacagcgactacaaagctatgaaaccctacattgacatagcggatgatattaaggcaaatgccatgaacaagtttaatcaactataaaagtatcaaacagtttcatagatagttaccaatgactgataggtgggctgcccttcctggttggcttggtttcatcagccatccgcttgccctcatctgttacgccggcggtagccggccagcctcgcagagcaggattcccgttgagcaccgccaggtgcgaataagggacagtgaagaaggaacacccgctcgcgggtgggcctacttcacctatcctgcccggctgacgccgttggatacaccaaggaaagtctacacgaaccctttggcaaaatcctgtatatcgtgcgaaaaaggatggatataccgaaaaaatcgctataatgaccccgaagcagggttatgcagcggaaaacggaattgatccggccacgatgcgtccggcgtagaggatctgaagatcagcgggattaaaagtcggggattggtgaacaaaaaggtgtttctctctttaagagaaatatcgttttgctaaacagttgatattgaggtatcattttatcgtaaaagacatttttgctcaacaattgcttgacggaaatcaacaaattttagcattttgtaaaaaagtcgctatataatttggtgaattggagttattttcatatttttgcatcccgaagagtttctcttaaagagagaaacatcttttgcataccttttccgaccgaatttttatgtcgtaaagaggggctttgcagggggtggactcagaaagatgagaatagatgactattgtagttgaaacacatagaaagttgctgatatacagaccgatacgcatatcgggatgaaccatgagtacgttcttttctcaaaaaacataaatattcgaaaagagatgcaataaattaaggagaggttataatgaacaaagtaaatataaaagatagtcaaaattttattacttcaaaatatcacatagaaaaaataatgaattgcataagtttagatgaaaaagataacatctttgaaataggtgcagggaaaggtcattttactgctggattggtaaagagatgtaattttgtaacggcgatagaaattgattctaaattatgtgaggtaactcgtaataagctcttaaattatcctaactatcaaatagtaaatgatgatatactgaaatttacatttcctagccacaatccatataaaatatttggcagcataccttacaacataagcacaaatataattcgaaaaattgtttttgaaagttcagccacaataagttatttaatagtggaatatggttttgctaaaatgttattagatacaaacagatcactagcattgctgttaatggcagaggtagatatttctatattagcaaaaattcctaggtattatttccatccaaaacctaaagtggatagcacattaattgtattaaaaagaaagccagcaaaaatggcatttaaagagagaaaaaaatatgaaacttttgtaatgaaatgggttaacaaagagtacgaaaaactgtttacaaaaaatcaatttaataaagctttaaaacatgcgagaatatatgatataaacaatattagtttcgaacaatttgtatcgctatttaatagttataaaatatttaacggctaaaaacaataggccacatgcaactgtaaatgtttacgcgggtaccgacaccgcggtggaggggaattatcacgtgctataaaaataattataatttaaattttttaatataaatatataaattaaaaatagaaagtaaaaaaagaaattaaagaaaaaatagtttttgttttccgaagatgtaaaagactctagggggatcgccaacaaatactaccttttatcttgctcttcctgctctcaggtattaatgccgaattgtttcatcttgtctgtgtagaagaccacacacgaaaatcctgtgattttacattttacttatcgttaatcgaatgtatatctatttaatctgcttttcttgtctaataaatatatatgtaaagtacgctttttgttgaaattttttaaacctttgtttatttttttttcttcattccgtaactcttctaccttctttatttactttctaaaatccaaatacaaaacataaaaataaataaacacagagtaaattcccaaattattccatcattaaaagatacgaggcgcgtgtaagttacaggcaagcgatccgtcagcttgcctcgtccccgccgggtcacccggccagcgacatggaggcccagaataccctccttgacagtcttgacgtgcgcagctcaggggcatgatgtgactgtcgcccgtacatttagcccatacatccccatgtataatcatttgcatccatacattttgatggccgcacggcgcgaagcaaaaattacggctcctcgctgcagacctgcgagcagggaaacgctcccctcacagacgcgttgaattgtccccacgccgcgcccctgtagagaaatataaaaggttaggatttgccactgaggttcttctttcatatacttccttttaaaatcttgctaggatacagttctcacatcacatccgaacataaacaaccatgggtaaggaaaagactcacgtttcgaggccgcgattaaattccaacatggatgctgatttatatgggtataaatgggctcgcgataatgtcgggcaatcaggtgcgacaatctatcgattgtatgggaagcccgatgcgccagagttgtttctgaaacatggcaaaggtagcgttgccaatgatgttacagatgagatggtcagactaaactggctgacggaatttatgcctcttccgaccatcaagcattttatccgtactcctgatgatgcatggttactcaccactgcgatccccggcaaaacagcattccaggtattagaagaatatcctgattcaggtgaaaatattgttgatgcgctggcagtgttcctgcgccggttgcattcgattcctgtttgtaattgtccttttaacagcgatcgcgtatttcgtctagctcaggcgcaatcacgaatgaataacggtttggttgatgcgagtgattttgatgacgagcgtaatggctggcctgttgaacaagtctggaaagaaatgcataagcttttgccattctcaccggattcagtcgtcactcatggtgatttctcacttgataaccttatttttgacgaggggaaattaataggttgtattgatgttggacgagtcggaatcgcagaccgataccaggatcttgccatcctatggaactgcctcggtgagttttctccttcattacagaaacggctttttcaaaaatatggtattgataatcctgatatgaataaa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cttgcattcctcagtagcaggtttaatggagcggtcggcactgataacaccatttatacagaagttttggtcgttgtgctcccctttctcatagtcaccaccataattccatgatttcttattatatttccgttcattatccagcaatccctggtctatccagtcccaaatatatccgccggcaagcgcatcatgagaacgtattgcatcccagtattctttcagcccgccggtagagtttcccatagaatgtgcatattcacacattattatcggacggttcatgaccggattcttagtcattgctataagctcatcgaccataggatacatacggctaatgacatcgacgtataaaggatcatcgggattggcatacacacaaagctctttctttgccggtttgacatcttcgttcacattaaaatctatctcactagtaacgattgacgcttccttacgtccgataggtttgtataaaggattttccggctgtccttgcgccccctcgtaatgaacaggacgggttgggtcataatctttcagccatcctgacagagctgcatgattagggccgcatccagactcgttgcccaacgaccacataaacacagaaggatggttcctgtctctcacagccattcttaccactctctccatgaacgagttagcccactcaggcctattggacagataccccctttgatgatgagtttcaagattagcctcatccattacgtatataccatacttatcgcacagttcatagaaataagggtcgttaggatagtgcgatgtacggactgtattgaagttataacgcttcataagcagaacgtcttcgagcatctcatcacgtgtaacggtcttacctccggtctcgctatggtcatggcggtttacaccaatgagtttaataggagtgtcattcaccagaatctgattacctgttattttaatatccctgaaccctaccttattacttctcgcatccaccacgttgccctttttgtctgtgagctttataaccaaagtgtatagataagggtgttccgaattccatagttttggcttagaaacaattccctccatcattccgtaataaacattatcacgctgaggataaggttcgttcaccacataatcggcagtaacggtaatgtcttttccaaacaccggtttcccatcggcatcatataattgggctgacagattccatcccttcaaatcatccatattctgatttgttatttccggacggatctgtaaccgtgctatattcttccggaaatcgatgcgtgtccttactccataatcatatattgccacctgcggaatggacatgatatatacttcacgatggataccagccattcgccagtggtcggcatcttccatataacttccgtcggtccacttatacacttgcaccgccagtttattctcccccttcttaacgtattcggtaatatcaaattcagtaggcagacaactgtcttcggaatatcccaccttctgtccgtttatccatacattaaatcccgaatagacgcctccgaaatggagtataatcctgtcgctcttccacttgtcaggaacaacaaactccttgatataacaccccgtctgattattcctgtcaatatatggcggacgagcagggaaaggataaatagtatttgtatatataggatagccatatccctgcatctcccaacatgaaggaacaggaatagttttccatgatgatgaattgtactccactttataaaaaccggcgggagccaatgccatatcctcggaaaagttaaacttccattggccgttcaacgacatatactccgatttctctctgtctccatccaaagcccaatccactctccggaaagaataagtagtactgcgggaaggcaaacggttaattccgtttatggtctgatcctgccatacattctgattgtttctccactgattggcaccgttgtccgatgcagacagaaattgcatcatgaaaaataacacagaaaatgaaaaaatagattttaagttcaagttcataaattcgcattttaagtttctatgcaaatatataagtataacgaacaatgaatagggggtatttctatctatatagagtggtatttttacatatgagctaaaacttaaaaaaaactgtcagtattactatgctatgtagcactctatatgaaaatattatatattcccaagtcaaaagccttttcaaacaatttttatatattctcatcctatcccttccatcaaagataaattccaatcctgatttgccagccgcatttattccttttttcaggagaattttctttatggctatcgccatgaaaattcacctgaaaaagaatgcggcggcaaacggattagaattaaagaaaagattacagggattaactgcgaccgacgtgacgcatagccgtaattcaaaggcggctatccttatattccatatatgacctcacaaatactgtgaaaatccactttccccaataacaaaacatagcctgccatatcaacacccaaaa
SEQ ID NO:74(pZR1622-氯霉素抗性载体)：
ACTAGAGATCTATGTGAGGATCCTAACTCGAGATCGAGACTGCTATGACTTGAGACCGGCTATTACGAGCGCTTAAACGGCGCGCCTGATAGGTGGGCTGCCCTTCCTGGTTGGCTTGGTTTCATCAGCCATCCGCTTGCCCTCATCTGTTACGCCGGCGGTAGCCGGCCAGCCTCGCAGAGCAGGATTCCCGTTGAGCACCGCCAGGTGCGAATAAGGGACAGTGAAGAAGGAACACCCGCTCGCGGGTGGGCCTACTTCACCTATCCTGCCCGGCTGACGCCGTTGGATACACCAAGGAAAGTCTACACGAACCCTTTGGCAAAATCCTGTATATCGTGCGAAAAAGGATGGATATACCGAAAAAATCGCTATAATGACCCCGAAGCAGGGTTATGCAGCGGAAAAGATAAAACGAAAGGCTCAGTCGAAAGACTGGGCCTTTCGTTTTACAATTGGGCTACCTTTTTTTTGTTTTGTTTGCAATGGTTAATCTATTGTTAAAATTTAAAGTTTCACTTGAACTTTCAAATAATGTTCTTATATTTGCAGTGTCGAAAGAAACAAAGTAGCCTGGATCACACAACATTTAAAAAATAACATTATGAAAGCACTCGAAAAGAGATTCAAAGGTAATATTAACAATAATTTATTTTCAATGGAGAAAAAAATCACTGGATATACCACCGTTGATATATCCCAATGGCATCGTAAAGAACATTTTGAGGCATTTCAGTCAGTTGCTCAATGTACCTATAACCAGACCGTTCAGCTGGATATTACGGCCTTTTTAAAGACCGTAAAGAAAAATAAGCACAAGTTTTATCCGGCCTTTATTCACATTCTTGCCCGCCTGATGAATGCTCATCCGGAATTTCGTATGGCAATGAAAGACGGTGAGCTGGTGATATGGGATAGTGTTCACCCTTGTTACACCGTTTTCCATGAGCAAACTGAAACGTTTTCATCGCTCTGGAGTGAATACCACGACGATTTCCGGCAGTTTCTACACATATATTCGCAAGATGTGGCGTGTTACGGTGAAAACCTGGCCTATTTCCCTAAAGGGTTTATTGAGAATATGTTTTTCGTTTCAGCCAATCCCTGGGTGAGTTTCACCAGTTTTGATTTAAACGTGGCCAATATGGACAACTTCTTCGCCCCCGTTTTCACCATGGGCAAATATTATACGCAAGGCGACAAGGTGCTGATGCCGCTGGCGATTCAGGTTCATCATGCCGTTTGTGATGGCTTCCATGTCGGCAGAATGCTTAATGAATTACAACAGTACTGCGATGAGTGGCAGGGCGGGGCGTAAGGTTCCTAGCTGATTAGAAGGCCATCCTGACGGATGGCCTTTTTTTTTGATAATAAGCGGATGAATGGCAGAAATTCGACGAGTCATTGGTAACTATCTATGAAACTGTTTGATACTTTTATAGTTGATTAAACTTGTTCATGGCATTTGCCTTAATATCATCCGCTATGTCAATGTAGGGTTTCATAGCTTTGTAGTCGCTGTGTCCCGTCCATTTCATGACCACCTGTGCCGGGATTCCGAGAGCCAGCGCATTGCAGATGAATGTCCTTTTTCCTGCATGGGTACTGAGCAAAGCGTATTTGGGTGTGACTTCATCAATACGTTCATTTCCCTTGTAGTAGGTTTCCCGTACAGGCTCGTTGATTTCTGCCAGTTCGCCCAGCTCTTTCAGGTAATCGTTCATCTTCTGGTTGCTGATGACGGGCAGAGCCATGTAATTCTCGAAATGGATGTCCTTGTATTTGTCCAGTATGGCTTTGCTGTATTTGTTCAGTTCAATCGTCAGGCTGTCGGCAGTCTTGACTGTGGTTATTTCGATGTGGTCGGACTTCACATCGCTTCTTTTCAGATTGCGAACATCCGAATACCGCAAACTCGTAAAGCAGCAGAACAGGAAAACATCACGCACACGTTCCAGGTATTGCTTATCCTTGGGTATCTGGTAGTCTTTCAGCTTGTTCAGTTCATCCCAAGTCAGGAAGATTACTTTTTTCGAGGTGGTTTTCAGTTTCGGTTTGAACGTATCGTATGCAATGTTCTGATGATGTCCTTTCTTGAAGCTCCAGCGCAGGAACCATTTGAGGAATCCCATTTGCTTGCCGATGGTGCTGTTTCTCATATCCTTGGTGTCACGCAGGAAGTTGACGTATTCGTTCAATCCAAACTCGTTGAAATAGTTGAACGTTGCATCCTCCTTGAACTCTTTGAGGTGGTTCCTCACTGCTGCAAATTTTTCATAGGTGGATGCCGTCCAGTTATTCTGGTTACCGCACTCTTTTACAAACTCATCGAACACCTCCCAAAAGCTGACAGGGGCTTCTTCCGGCTGTTCTTCACTGGTATCTTTCATTCTCATGTTGAAAGCTTCCTTCAACTGTTGGGTCGTTGGCATGACCTCCTGCACCTCAAATTCCTTGAAAATATTCTGGATTTCGGCATAGTATTTCAGCAAGTCCGTATTGATTTCGGCTGCACTTTGCTTTAGCTTGTTGGTACATCCGTTCTTTACCCGCTGCTTATCTGCATCCCATTTGGCTACGTCAATCCGGTAGCCCGTTGTAAACTCGATACGTTGGCTGGCAAAGATGACACGCATACGGATGGGTACGTTCTCTACGATTGGCACACCGTTCTTTTTCCGGCTCTCCAATGCAAAAATGATGTTGCGCTTGATATTCATAATTGGGTGCGTTTGAAATTCTACACCCAAATATACACCCAATTATTGAGATAGCAAAAGACATTTAGAAACATTTACTTTTACTCTATATTGTAATTTACACTTGATTATCAGTCGTTTGCAGTCTTATGATATTCTGTGAAAGTATAAGTTCGAGAGCCTGTCTCTCCGCAAAAAACGCTGAAAATCAGCAGATTGCAAAACAAACACCCTGTTTTACACCCAAGAATGTAAAGTCGGGTGTTTTTGTTTTATTTAAGATAATACAACCACTACATAATAAAAGAGTAGCGATATTAAAAGAATCCGATGAGAAAAGACTAATATTTATCTATCCATTCAGTTTGATTTCTCAGGACTTTACATCGTCCTGAAAGTATTTGTTGCCAGTGTTACAACCAATTAACCAATTCTGATTAGAAAAACTCATCGAGCATCAAATGAAACTGCAATTTATTCATATCAGGATTATCAATACCATATTTTTGAAAAAGCCGTTTCTGTAATGAAGGAGAAAACTCACCGAGGCAGTTCCATAGGATGGCAAGATCCTGGTATCGGTCTGCGATTCCGACTCGTCCAACATCAATACAACCTATTAATTTCCCCTCGTCAAAAATAAGGTTATCAAGTGAGAAATCACCATGAGTGACGACTGAATCCGGTGAGAATGGCAAAAGCTTATGCATTTCTTTCCAGACTTGTTCAACAGGCCAGCCATTACGCTCGTCATCAAAATCACTCGCATCAACCAAACCGTTATTCATTCGTGATTGCGCCTGAGCGAGGCGAAATACGCGATCGCTGTTAAAAGGACAATTACAAACAGGAATCGAATGCAACCGGCGCAGGAACACTGCCAGCGCATCAACAATATTTTCACCTGAATCAGGATATTCTTCTAATACCTGGAATGCTGTTTTCCCGGGGATCGCAGTGGTGAGTAACCATGCATCATCAGGAGTACGGATAAAATGCTTGATGGTCGGAAGAGGCATAAATTCCGTCAGCCAGTTTAGTCTGACCATCTCATCTGTAACATCATTGGCAACGCTACCTTTGCCATGTTTCAGAAACAACTCTGGCGCATCGGGCTTCCCATACAATCGATAGATTGTCGCACCTGATTGCCCGACATTATCGCGAGCCCATTTATACCCATATAAATCAGCATCCATGTTGGAATTTAATCGCGGCCTGGAGCAAGACGTTTCCCGTTGAATATGGCTCATAACACCCCTTGTATTACTGTTTATGTAAGCAGACAGTTTTATTGTTCATGATGATATATTTTTATCTTGTGCAATGTAACATCAGAGATTTTGAGACACAACGTGGCTTTGTTGAATAAATCGAACTTTTGCTGAGTTGAAGGATCAGCCGCGCAGTTCAACCTGTTGATAGTACGTACTAAGCTCTCATGTTTCACGTACTAAGCTCTCATGTTTAACGTACTAAGCTCTCATGTTTAACGAACTAAACCCTCATGGCTAACGTACTAAGCTCTCATGGCTAACGTACTAAGCTCTCATGTTTCACGTACTAAGCTCTCATGTTTGAACAATAAAATTAATATAAATCAGCAACTTAAATAGCCTCTAAGGTTTTAAGTTTTATAAGAAAAAAAAGAATATATAAGGCTTTTAAAGCTTTTAAGGTTTAACGGTTGTGGACAACAAGCCAGGGATGTAACGCACTGAGAAGCCCTTAGAGCCTCTCAAAGCAATTTTGAGTGACACAGGAACACTTAACGGCTGACATGGGGCGGCCGCTCAACGTACCGGTCTCAGTAGGGAGAGCTGTATGTGGGTAG
SEQ ID NO:75(pZR0879-Ec_frc,Of_oxc,Sc_YBR222C,Sc_YEL020C,Ec_yfdE,Of_oxlT(高强度启动子)表达载体)：
GGAGAGCTGTATGTGGGTAGGATAAAACGAAAGGCTCAGTCGAAAGACTGGGCCTTTCGTTTTACAATTGGGCTACCTTTTTTTTGTTTTGTTTGCAATGGTTAATCTATTGTTAAAATTTAAAGTTTCACTTGAACTTTCAAATAATGTTCTTATATTTGCAGTGTCGAAAGAAACAAAGTAGCCTGGATCACACAACATTTAAAAAATAACATTATGAAAGCACTCGAAAAGAGATTCAAAGGTAATATTAACAATAATTTATTTTCAATGTCAACTCCACTTCAAGGAATTAAAGTTCTCGATTTCACCGGTGTGCAATCTGGCCCATCTTGTACTCAAATGCTGGCCTGGTTTGGCGCTGACGTTATTAAAATTGAACGTCCCGGCGTTGGTGACGTAACGCGTCACCAGCTGCGAGATATTCCTGATATCGATGCGCTTTACTTCACCATGCTTAACAGTAACAAACGTTCTATTGAGTTAAATACCAAAACAGCGGAAGGCAAAGAGGTAATGGAAAAGCTGATCCGCGAAGCTGATATCTTAGTCGAGAACTTTCATCCAGGGGCCATTGATCACATGGGCTTCACCTGGGAGCATATTCAAGAAATCAATCCACGTCTGATTTTTGGTTCGATCAAAGGGTTTGATGAGTGTTCGCCTTATGTGAATGTAAAAGCCTATGAAAACGTTGCTCAGGCAGCGGGTGGCGCGGCATCCACTACGGGTTTTTGGGATGGTCCGCCGCTGGTAAGCGCTGCAGCGTTGGGTGACAGCAACACCGGAATGCATTTGCTGATCGGTTTACTTGCTGCTTTGCTGCATCGCGAAAAAACGGGGCGTGGGCAACGAGTCACCATGTCAATGCAGGATGCCGTATTGAACCTTTGCCGCGTGAAATTACGTGACCAGCAGCGTCTAGATAAATTGGGTTATCTGGAAGAATACCCGCAGTATCCGAATGGTACATTTGGTGATGCAGTTCCCCGCGGTGGTAATGCAGGTGGTGGCGGTCAGCCTGGCTGGATCCTGAAATGTAAAGGCTGGGAAACCGATCCTAACGCCTATATTTATTTCACTATTCAGGAGCAAAACTGGGAAAACACCTGTAAAGCCATCGGCAAACCAGAATGGATTACCGATCCGGCATACAGTACAGCCCATGCACGACAGCCACATATTTTCGATATTTTTGCTGAAATCGAAAAATACACTGTCACTATTGATAAACATGAAGCGGTGGCCTATTTGACTCAGTTTGATATTCCTTGTGCACCGGTTTTAAGTATGAAAGAAATTTCACTTGATCCCTCTTTGCGCCAAAGTGGCAGTGTTGTTGAAGTGGAACAACCGTTGCGTGGAAAATATCTGACCGTTGGTTGTCCAATGAAATTCTCTGCCTTTACGCCGGATATTAAAGCTGCGCCGCTATTAGGTGAACATACCGCTGCTGTATTGCAGGAGCTGGGTTATAGCGACGATGAAATTGCTGCAATGAAGCAAAACCACGCCATCTGAGGTTCCTAGCTGATTAGAAGGCCATCCTGACGGATGGCCTTTTTTTTGACTGGTAAGCCAATCTAGGGCCTCGTCTGTAGGATAAAACGAAAGGCTCAGTCGAAAGACTGGGCCTTTCGTTTTACAATTGGGCTACCTTTTTTTTGTTTTGTTTGCAATGGTTAATCTATTGTTAAAATTTAAAGTTTCACTTGAACTTTCAAATAATGTTCTTATATTTGCAGTGTCGAAAGAAACAAAGTAGCCTGAGCTGTCACCGGATGTGCTTTCCGGTCTGATGAGTCCGTGAGGACGAAACAGCCTCTACAAATAATTTTGTTTAATCCATCAATTTAAAATTTAAAATAATGGTTTTTACTCTGGAAGATTTTGTTGGCGATTGGCGTCAGACCGCGGGTTATAATTTGGATCAAGTCCTGGAACAGGGTGGCGTAAGCTCTCTGTTCCAGAACCTGGGTGTGAGCGTGACGCCGATTCAGCGCATCGTTCTGTCCGGCGAGAACGGTCTGAAAATTGATATTCATGTGATCATCCCGTACGAAGGCCTGAGCGGTGACCAAATGGGTCAAATCGAGAAAATCTTTAAAGTCGTCTACCCAGTTGACGATCACCACTTCAAGGTTATCTTGCATTACGGTACGCTGGTGATTGATGGTGTGACCCCGAATATGATTGACTATTTCGGCCGTCCGTATGAAGGCATTGCCGTTTTTGACGGTAAAAAGATCACCGTCACCGGTACCCTGTGGAATGGCAATAAGATTATTGACGAGCGTCTGATTAACCCGGACGGCAGCCTGCTGTTCCGCGTGACCATCAACGGTGTCACGGGTTGGCGTCTGTGCGAGCGCATCCTGGCAGGTTCAATGAGTAACGACGACAATGTAGAGTTGACTGATGGCTTTCATGTTTTGATCGATGCCCTGAAAATGAATGACATCGATACCATGTATGGTGTTGTCGGCATTCCTATCACGAACCTGGCTCGTATGTGGCAAGATGACGGTCAGCGTTTTTACAGCTTCCGTCACGAACAACACGCAGGTTATGCAGCTTCTATCGCCGGTTACATCGAAGGAAAACCTGGCGTTTGCTTGACCGTTTCCGCCCCTGGCTTCCTGAACGGCGTGACTTCCCTGGCTCATGCAACCACCAACTGCTTCCCAATGATCCTGTTGAGCGGTTCCAGTGAACGTGAAATCGTCGATTTGCAACAGGGCGATTACGAAGAAATGGATCAGATGAATGTTGCACGTCCACACTGCAAAGCTTCTTTCCGTATCAACAGCATCAAAGACATTCCAATCGGTATCGCTCGTGCAGTTCGCACCGCTGTATCCGGACGTCCAGGTGGTGTTTACGTTGACTTGCCAGCAAAACTGTTCGGTCAGACCATTTCTGTAGAAGAAGCTAACAAACTGCTCTTCAAACCAATCGATCCAGCTCCGGCACAGATTCCTGCTGAAGATGCTATCGCTCGCGCTGCTGACCTGATCAAGAACGCCAAACGTCCAGTTATCATGCTGGGTAAAGGCGCTGCATACGCACAATGCGACGACGAAATCCGCGCACTGGTTGAAGAAACCGGCATCCCATTCCTGCCAATGGGTATGGCTAAAGGCCTGCTGCCTGACAACCATCCACAATCCGCTGCTGCAACCCGTGCTTTCGCACTGGCACAGTGTGACGTTTGCGTACTGATCGGCGCTCGTCTGAACTGGCTGATGCAGCACGGTAAAGGCAAAACCTGGGGCGACGAACTGAAGAAATACGTTCAGATCGACATCCAGGCTAACGAAATGGACAGCAACCAGCCTATCGCTGCACCAGTTGTTGGTGACATCAAGTCCGCCGTTTCCCTGCTCCGCAAAGCACTGAAAGGCGCTCCAAAAGCTGACGCTGAATGGACCGGCGCTCTGAAAGCCAAAGTTGACGGCAACAAAGCCAAACTGGCTGGCAAGATGACTGCCGAAACCCCATCCGGAATGATGAACTACTCCAATTCCCTGGGCGTTGTTCGTGACTTCATGCTGGCAAATCCGGATATTTCCCTGGTTAACGAAGGCGCTAATGCACTCGACAACACTCGTATGATTGTTGACATGCTGAAACCACGCAAACGTCTTGACTCCGGTACCTGGGGTGTTATGGGTATTGGTATGGGCTACTGCGTTGCTGCAGCTGCTGTTACCGGCAAACCGGTTATCGCTGTTGAAGGCGATAGCGCATTCGGTTTCTCCGGTATGGAACTGGAAACCATCTGCCGTTACAACCTGCCAGTTACCGTTATCATCATGAACAATGGTGGTATCTATAAAGGTAACGAAGCAGATCCACAACCAGGCGTTATCTCCTGTACCCGTCTGACCCGTGGTCGTTACGACATGATGATGGAAGCATTTGGCGGTAAAGGTTATGTTGCCAATACTCCAGCAGAACTGAAAGCTGCTCTGGAAGAAGCTGTTGCTTCCGGCAAACCATGCCTGATCAACGCGATGATCGATCCAGACGCTGGTGTCGAATCTGGCCGTATCAAGAGCCTGAACGTTGTAAGTAAAGTTGGCAAGAAATAAGGTTCCTAGCTGATTAGAAGGCCATCCTGACGGATGGCCTTTTTTTTGACTGCGTTCCTGGCGTGGTGCTCGCATAGTAGGATAAAACGAAAGGCTCAGTCGAAAGACTGGGCCTTTCGTTTTACAATTGGGCTACCTTTTTTTTGTTTTGTTTGCAATGGTTAATCTATTGTTAAAATTTAAAGTTTCACTTGAACTTTCAAATAATGTTCTTATATTTGCAGTGTCGAAAGAAACAAAGTAGCCTGGATCACACAACATTTAAAAAATAACATTATGAAAGCACTCGAAAAGAGATTCAAAGGTAATATTAACAATAATTTATTTTCAATGACAAGTGCCGCTACTGTTACTGCTTCGTTCAACGATACTTTTAGCGTATCCGATAATGTCGCCGTTATTGTTCCTGAAACGGACACTCAGGTGACCTACAGGGATCTATCCCACATGGTGGGTCACTTCCAGACCATGTTCACAAATCCTAATTCTCCATTGTACGGAGCTGTTTTCAGACAAGATACAGTGGCGATATCCATGCGTAATGGGCTGGAATTTATCGTCGCTTTCCTCGGTGCTACTATGGACGCTAAAATTGGCGCGCCCTTGAATCCCAATTATAAGGAAAAGGAGTTCAATTTTTATTTGAATGACCTGAAATCTAAGGCGATTTGCGTCCCAAAGGGTACCACAAAGTTACAGAGTTCTGAAATTCTAAAATCTGCCTCCACGTTTGGATGTTTTATCGTAGAGCTGGCCTTCGATGCGACCAGGTTTAGGGTAGAGTATGATATATACTCTCCAGAGGACAACTACAAAAGGGTTATTTACCGGTCTTTGAACAACGCCAAATTTGTCAACACAAATCCCGTTAAATTCCCTGGGTTTGCCCGTTCCAGTGACGTTGCCCTGATTTTGCATACCAGTGGTACCACCTCCACTCCAAAAACGGTGCCTTTGTTACATTTGAACATTGTGAGAAGCACGTTGAACATTGCTAACACTTACAAGCTAACGCCCTTGGACAGATCTTATGTCGTGATGCCTCTTTTCCACGTCCATGGGTTAATTGGTGTTTTACTTTCCACTTTTAGAACTCAGGGTTCTGTTGTGGTTCCCGATGGATTCCATCCAAAGTTATTCTGGGACCAATTTGTTAAGTACAACTGTAATTGGTTCAGTTGCGTTCCCACAATAAGCATGATTATGCTGAACATGCCCAAACCAAACCCTTTCCCACACATTAGATTCATCAGATCGTGTTCTTCTGCTTTGGCTCCAGCAACGTTCCATAAGCTGGAGAAGGAATTCAATGCACCTGTCTTGGAGGCCTATGCGATGACCGAAGCATCACATCAAATGACCTCAAACAATCTGCCTCCAGGAAAGAGAAAGCCTGGTACTGTGGGCCAGCCACAAGGAGTCACCGTCGTCATTCTAGATGACAATGACAATGTCTTGCCCCCGGGCAAAGTCGGCGAAGTTTCCATCAGAGGCGAAAACGTCACTTTGGGGTATGCTAATAATCCAAAAGCTAACAAGGAGAACTTCACCAAGAGAGAGAACTATTTCAGAACCGGTGACCAAGGTTATTTCGACCCTGAGGGGTTTTTGGTCCTTACAGGCAGAATCAAAGAGCTTATCAACAGGGGTGGTGAAAAGATTTCACCCATTGAGCTCGACGGCATTATGCTATCGCATCCAAAGATCGATGAAGCCGTTGCATTTGGTGTTCCCGACGATATGTACGGCCAAGTAGTTCAAGCCGCCATTGTTTTGAAGAAGGGAGAAAAAATGACCTACGAAGAACTGGTGAACTTCTTAAAGAAGCACCTAGCCTCTTTCAAAATTCCAACCAAGGTGTACTTTGTTGATAAGCTACCAAAAACCGCTACAGGTAAAATCCAGAGAAGAGTTATCGCAGAAACTTTTGCTAAGAGCAGCAGAAATAAGAGTAAGTTGTAGGGTTCCTAGCTGATTAGAAGGCCATCCTGACGGATGGCCTTTTTTTTGACTGCCCGACGAACGGACTAATCCTGACGTAGGATAAAACGAAAGGCTCAGTCGAAAGACTGGGCCTTTCGTTTTACAATTGGGCTACCTTTTTTTTGTTTTGTTTGCAATGGTTAATCTATTGTTAAAATTTAAAGTTTCACTTGAACTTTCAAATAATGTTCTTATATTTGCAGTGTCGAAAGAAACAAAGTAGCCTGGATCACACAACATTTAAAAAATAACATTATGAAAGCACTCGAAAAGAGATTCAAAGGTAATATTAACAATAATTTATTTTCAATGACCACGACCGCTACCCAACACTTCGCTCAACTTTTACAAAAATATGGCATCGACACAGTCTTTGGAATTGTGGGCATCCCTATTGTCCAACTAGCGGATACGATGGTTGCCAACGGTATCAAATTCATTCCATGCAGGAACGAACAGGCTGCCTCATATGCAGCCTCTGCTTATGGTTATATTAGTGATAAGCCCGGCGTGTTACTTATTGTCGGGGGCCCCGGTTTAATCCACGCACTGGCTGGTATATACAACTCAATGAGTAATAGGTGGCCTCTTCTAGTAATTGCAGGGAGTTCTTCTCAAAGTGATATACATAAAGGCGGGTTTCAAGAGTTGGATCAAGTTAGTCTTTTGTCCCCATTTTTGAAGTTTACCGGAAAGTTAACCCCTGACAATATTGACATGATTACTCAGAAGGCTTTGAATTACTGTATACAAGGCACTGCAGGGGTTTCTTACATAGACGTCCCTGCAGATTTTATTGAGTATGAAAAACCCTTAGAAGGAAACGACCGTACAGGAAATGAATTACCGATGATATTAACTCCAAACATATGTGGCCCTGATCCGTCGAAGATCAAAAAAGTCGTACAGCTTATTTTGCAACATAAAAATAAAAATATTCTGATTGTTATTGGAAAGGGTGCGGTAAAAAACTCACACGAAATCCGCAGACTAGTAAATACGTTCAATCTACCATTTTTACCCACCCCAATGGCTAAGGGAATTGTCCCAGACTCTTCTCCACTGAACGTTTCATCTGCAAGATCTCAAGCATTGAAAATAGCGGATATTGTTCTTGTTCTCGGGGCAAGATTAAACTGGATATTGCATTTTGGTACTTCACCCAAATGGAACTCAGAATCCATATTCATCCAGTTCGATTCCAATCCCGAAACCTTGGGGGATAACAATGTCTCACCTGGTGCTGACCTTTCCATATGGGGAGATATAGGCTTGAGCGTAACCGCTTTAGTTGAGGAATTGACTCGCCAAGATTCCTGCTGGAAGTATAGTGGTGTTAAGCAAGAAATACGAGAGAAAATTCAACTGAATCAAACTCGTCTATTGAGGAAAGAGAAGACTAGAGGGGCACAATTGAATTACAACCAGGTTTATGGAACATTGAGGCCTCTTATCGATGATTACAGGACAATACTTGTGACGGAGGGAGCAAATACTATGGATATTGCGCGTATTTCATTCCCTACAGACGCTCCAAGGCGCCGTTTAGACGCAGGGACCAACGCGACTATGGGGATTGGGCTCGGATATGCTCTTGCATGCAAGGCATCTCATCCGGAACTCGATGTGGTGCTGATTCAGGGTGATTCCGCATTTGGATTCTCTGCCATGGAAATTGAAACGGCAGTAAGGTGTCAGCTGGCATTGGTCATCGTTGTGATGAACAATAGCGGTATTTACCATGGAGAAAAGGATATAGAGGGTGATTTACCTCCAACGGCACTAAGCAAGAACTGCCGTTACGATCTCGTAGGCAAAGGACTGGGTGCCAATGATTTTTTTGTCAACACAATAAGCGAACTGAGCAGATGCTTCCAACAAGCCGTGCAGCTTTCTCGGACTAAAAGAGAAACAAGTGTCATCAACGTTATCATTGAGCCTGGCGAACAAAAGCAAATTGCCTTTGCCTGGCAGAATAAACCGCGTTTATAGGGTTCCTAGCTGATTAGAAGGCCATCCTGACGGATGGCCTTTTTTTTGACTGCTCTGGGTCTGACGGCCATCAAGGGTAGTGAGACTAGCTGATTAGAAGGCCATCCTGACGGATGGCCTTTTTTTTGACTGGGTGTTCCGATACACTCAATTTGTGTAGGATAAAACGAAAGGCTCAGTCGAAAGACTGGGCCTTTCGTTTTACAATTGGGCTACCTTTTTTTTGTTTTGTTTGCAATGGTTAATCTATTGTTAAAATTTAAAGTTTCACTTGAACTTTCAAATAATGTTCTTATATTTGCAGTGTCGAAAGAAACAAAGTAGCCTGGATCACACAACATTTAAAAAATAACATTATGAAAGCACTCGAAAAGAGATTCAAAGGTAATATTAACAATAATTTATTTTCAATGACAAATAATGAAAGCAAAGGGCCGTTTGAAGGCTTATTAGTTATCGATATGACACATGTCCTTAATGGACCTTTCGGAACTCAACTTCTTTGTAATATGGGCGCAAGGGTAATTAAAGTTGAGCCGCCGGGTCATGGTGATGATACCCGCACATTTGGTCCCTATGTGGATGGACAGTCACTCTATTACAGTTTTATTAATCATGGCAAAGAGAGTGTGGTTCTTGATTTAAAGAATGATCACGATAAAAGTATATTTATAAATATGCTCAAACAAGCTGATGTATTAGCTGAGAATTTTCGCCCAGGTACAATGGAAAAACTGGGGTTTTCATGGGAAACGCTTCAAGAAATCAACCCGCGCCTCATATATGCTTCATCGTCAGGTTTCGGACATACCGGTCCGCTAAAAGATGCTCCTGCCTACGATACCATCATTCAGGCAATGAGCGGGATAATGATGGAAACAGGATATCCTGATGCTCCGCCAGTGCGCGTTGGTACATCTCTTGCGGATCTATGCGGCGGTGTCTATTTATTCAGCGGAATAGTGAGTGCACTTTATGGCCGCGAAAAGAGCCAGAGAGGGGCGCATGTCGATATAGCGATGTTTGATGCCACGCTGAGTTTTCTGGAGCATGGTCTGATGGCATATATCGCAACTGGGAAGTCACCACAACGTCTGGGAAATCGCCATCCCTACATGGCACCTTTTGATGTTTTCAATACTCAGGATAAGCCGATTACGATTTGTTGTGGTAATGACAAGCTTTTTTCTGCGTTATGCCAGGCACTGGAGCTTACGGAACTGGTTAATGATCCCCGATTTAGCAGCAATATTTTACGCGTACAAAACCAGGCTATTCTTAAACAATATATTGAGCGGACGTTAAAAACGCAGGCAGCTGAAGTTTGGTTAGCCAGAATACATGAAGTTGGTGTACCCGTCGCGCCGTTATTAAGTGTGGCTGAGGCCATTAAATTGCCACAAACTCAGGCGAGAAATATGTTGATTGAAGCCGGGGGAATAATGATGCCGGGTAATCCGATAAAAATCAGCGGCTGCGCGGACCCGCATGTTATGCCGGGAGCGGCAACGCTCGACCAGCATGGGGAACAAATTCGCCAGGAGTTCTCATCATAAGGTTCCTAGCTGATTAGAAGGCCATCCTGACGGATGGCCTTTTTTTTGACTTAGTTTCAATTTTACATTCCAGGAAGTAGGATAAAACGAAAGGCTCAGTCGAAAGACTGGGCCTTTCGTTTTACAATTGGGCTACCTTTTTTTTGTTTTGTTTGCAATGGTTAATCTATTGTTAAAATTTAAAGTTTCACTTGAACTTTCAAATAATGTTCTTATATTTGCAGTGTCGAAAGAAACAAAGTAGCCTGGATCACACAACATTTAAAAAATAACATTATGAAAGCACTCGAAAAGAGATTCAAAGGTAATATTAACAATAATTTATTTTAATGAATAATCCACAAACAGGACAATCAACAGGCCTCTTGGGCAATCGTTGGTTCTACTTGGTATTAGCAGTTTTGCTGATGTGTATGATCTCGGGTGTCCAATATTCCTGGACACTGTACGCTAACCCGGTTAAAGACAACCTTGGCGTTTCTTTGGCTGCGGTTCAGACGGCTTTCACACTCTCTCAGGTCATTCAAGCTGGTTCTCAGCCTGGTGGTGGTTACTTCGTTGATAAATTCGGTCCAAGAATTCCATTGATGTTCGGTGGTGCGATGGTTCTCGCTGGCTGGACCTTCATGGGTATGGTTGACAGTGTTCCTGCTCTGTATGCTCTTTATACTCTGGCCGGTGCAGGTGTTGGTATCGTTTACGGTATCGCGATGAACACGGCTAACAGATGGTTCCCGGACAAACGCGGTCTGGCTTCCGGTTTCACCGCTGCCGGTTACGGTCTGGGTGTTCTGCCGTTCCTGCCACTGATCAGCTCCGTTCTGAAAGTTGAAGGTGTTGGCGCAGCATTCATGTACACCGGTTTGATCATGGGTATCCTGATTATCCTGATCGCTTTCGTTATCCGTTTCCCTGGCCAGCAAGGCGCCAAAAAACAAATCGTTGTTACCGACAAGGATTTCAATTCTGGCGAAATGCTGAGAACACCACAATTCTGGGTTCTGTGGACCGCATTCTTTTCCGTTAACTTTGGTGGTTTGCTGCTGGTTGCCAACAGCGTCCCTTACGGTCGCAGCCTCGGTCTTGCCGCAGGTGTGCTGACGATCGGTGTTTCGATCCAGAACCTGTTCAATGGTGGTTGCCGTCCTTTCTGGGGTTTCGTTTCCGATAAAATCGGCCGTTACAAAACCATGTCCGTCGTTTTCGGTATCAATGCTGTTGTTCTCGCACTTTTCCCGACGATTGCTGCCTTGGGCGATGTAGCCTTTATCGCCATGTTGGCAATCGCATTCTTCACATGGGGTGGTAGCTACGCTCTGTTCCCATCGACCAACAGCGATATTTTCGGTACGGCATACTCTGCCAGAAACTATGGTTTCTTCTGGGCTGCAAAAGCAACTGCCTCGATCTTCGGTGGTGGTCTGGGTGCTGCAATTGCAACCAACTTCGGATGGAATACCGCTTTCCTGATTACTGCGATTACTTCTTTCATCGCATTTGCTCTGGCTACCTTCGTTATTCCAAGAATGGGCCGTCCAGTCAAGAAAATGGTCAAATTGTCTCCAGAAGAAAAAGCTGTACATTAAGGTTCCTAGCTGATTAGAAGGCCATCCTGACGGATGGCCTTTTTTTTGACTGCTATGACTAAACGGCGCGCCTGATAGGTGGGCTGCCCTTCCTGGTTGGCTTGGTTTCATCAGCCATCCGCTTGCCCTCATCTGTTACGCCGGCGGTAGCCGGCCAGCCTCGCAGAGCAGGATTCCCGTTGAGCACCGCCAGGTGCGAATAAGGGACAGTGAAGAAGGAACACCCGCTCGCGGGTGGGCCTACTTCACCTATCCTGCCCGGCTGACGCCGTTGGATACACCAAGGAAAGTCTACACGAACCCTTTGGCAAAATCCTGTATATCGTGCGAAAAAGGATGGATATACCGAAAAAATCGCTATAATGACCCCGAAGCAGGGTTATGCAGCGGAAAAGTTATATACATTCATGTCCATTTATGTAAAAAATCCTGCTGACCTTGTTTATGTCTTGTCAGTCACCATTTGCAAAACCATATTTGACCCTCAAAGAGGCTGAATTTGATAAGCAACTTGCTACATACTCATAATAAGGAGCTAAATAGAACACGAATGGGAAATACTCAAATGCCAAACTAAAGAAGATATTGGCCAAAATAAACGCTATACCGAGAGAGAAACTTGATTTTTCAACTTCCTAAAACAGTGTTGTTCAAACATTTCTACTTATTTGTACTTACCAGTTGAACCTACGTTTCCCTAATAAAATGTCTATGGTAAAAAGTTAAAAAATCCTCCTACTTTTGTTAGATATATTTTTTTGTGTAATTTTGTAATCGTTATGCGGCAGTAATAATATACATATTAATACGAGTTAGGAATCCTGTAGTTCTCATATGCTACGAGGAGGTATTAAAAGGTGCGTTTCGACAATGCATCTATTGTAGTATATTATTGCTTAATCCAAATGAATATTATAAATTTAGGAATTCTTGCTCACATTGATGCAGGAAAAACTTCCGTAACCGAGAATCTGCTGTTTGCCAGTGGAGCAACGGAAAAGTGCGGCTGTGTGGATAATGGTGACACCATAACGGACTCTATGGATATAGAGAAACGTAGAGGAATTACTGTTCGGGCTTCTACGACATCTATTATCTGGAATGGTGTGAAATGCAATATCATTGACACTCCGGGACACATGGATTTTATTGCGGAAGTGGAGCGGACATTCAAAATGCTTGATGGAGCAGTCCTCATCTTATCCGCAAAGGAAGGCATACAAGCGCAGACAAAGTTGCTGTTCAATACTTTACAGAAGCTGCAAATCCCGACAATTATATTTATCAATAAGATTGACCGAGCCGGTGTGAATTTGGAGCGTTTGTATCTGGATATAAAAGCAAATCTGTCTCAAGATGTCCTGTTTATGCAAAATGTTGTCGATGGATCGGTTTATCCGGTTTGCTCCCAAACATATATAAAGGAAGAATACAAAGAATTTGTATGCAACCATGACGACAATATATTAGAACGATATTTGGCGGATAGCGAAATTTCACCGGCTGATTATTGGAATACGATAATCGCTCTTGTGGCAAAAGCCAAAGTCTATCCGGTGCTACATGGATCAGCAATGTTCAATATCGGTATCAATGAGTTGTTGGACGCCATCACTTCTTTTATACTTCCTCCGGCATCGGTTTCAAACAGACTTTCATCTTATCTTTATAAGATAGAGCATGACCCCAAAGGACATAAAAGAAGTTTTCTAAAAATAATTGACGGAAGTCTGAGACTTCGAGATGTTGTAAGAATCAACGATTCGGAAAAATTCATCAAGATTAAAAATCTAAAAACTATCAATCAGGGCAGAGAGATAAATGTTGATGAAGTGGGCGCCAATGATATCGCGATTGTAGAGGATATGGATGATTTTCGAATCGGAAATTATTTAGGTGCTGAACCTTGTTTGATTCAAGGATTATCGCATCAGCATCCCGCTCTCAAATCCTCCGTCCGGCCAGACAGGCCCGAAGAGAGAAGCAAGGTGATATCCGCTCTGAATACATTGTGGATTGAAGATCCGTCTTTGTCCTTTTCCATAAACTCATATAGTGATGAATTGGAAATCTCGTTATATGGTTTAACCCAAAAGGAAATCATACAGACATTGCTGGAAGAACGATTTTCCGTAAAGGTCCATTTTGATGAGATCAAGACTATATACAAAGAACGACCTGTAAAAAAGGTCAATAAGATTATTCAGATCGAAGTGCCGCCCAACCCTTATTGGGCCACAATAGGGCTGACTCTTGAACCCTTACCGTTAGGGACAGGGTTGCAAATCGAAAGTGACATCTCCTATGGTTATCTGAACCATTCTTTTCAAAATGCCGTTTTTGAAGGGATTCGTATGTCTTGCCAATCCGGGTTACATGGATGGGAAGTGACTGATCTGAAAGTAACTTTTACTCAAGCCGAGTATTATAGCCCGGTAAGTACACCAGCTGATTTCAGACAGCTGACCCCTTATGTCTTTAGGCTGGCCTTGCAACAGTCAGGTGTGGACATTCTCGAACCGATGCTCTATTTTGAGTTGCAGATACCCCAAGCGGCAAGTTCCAAAGCTATTACAGATTTGCAAAAAATGATGTCTGAGATTGAAGATATCAGTTGCAATAATGAGTGGTGTCATATTAAAGGGAAAGTTCCATTAAATACAAGTAAAGACTATGCATCAGAAGTAAGTTCATACACTAAGGGCTTAGGCATTTTTATGGTTAAGCCATGCGGGTATCAAATAACAAAAGGCGGTTATTCTGATAATATCCGCATGAACGAAAAAGATAAACTTTTATTCATGTTCCAAAAATCAATGTCATCAAAATAACCACGAGTCATTGGTAACTATCTATGAAACTGTTTGATACTTTTATAGTTGATTAAACTTGTTCATGGCATTTGCCTTAATATCATCCGCTATGTCAATGTAGGGTTTCATAGCTTTGTAGTCGCTGTGTCCCGTCCATTTCATGACCACCTGTGCCGGGATTCCGAGAGCCAGCGCATTGCAGATGAATGTCCTTTTTCCTGCATGGGTACTGAGCAAAGCGTATTTGGGTGTGACTTCATCAATACGTTCATTTCCCTTGTAGTAGGTTTCCCGTACAGGCTCGTTGATTTCTGCCAGTTCGCCCAGCTCTTTCAGGTAATCGTTCATCTTCTGGTTGCTGATGACGGGCAGAGCCATGTAATTCTCGAAATGGATGTCCTTGTATTTGTCCAGTATGGCTTTGCTGTATTTGTTCAGTTCAATCGTCAGGCTGTCGGCAGTCTTGACTGTGGTTATTTCGATGTGGTCGGACTTCACATCGCTTCTTTTCAGATTGCGAACATCCGAATACCGCAAACTCGTAAAGCAGCAGAACAGGAAAACATCACGCACACGTTCCAGGTATTGCTTATCCTTGGGTATCTGGTAGTCTTTCAGCTTGTTCAGTTCATCCCAAGTCAGGAAGATTACTTTTTTCGAGGTGGTTTTCAGTTTCGGTTTGAACGTATCGTATGCAATGTTCTGATGATGTCCTTTCTTGAAGCTCCAGCGCAGGAACCATTTGAGGAATCCCATTTGCTTGCCGATGGTGCTGTTTCTCATATCCTTGGTGTCACGCAGGAAGTTGACGTATTCGTTCAATCCAAACTCGTTGAAATAGTTGAACGTTGCATCCTCCTTGAACTCTTTGAGGTGGTTCCTCACTGCTGCAAATTTTTCATAGGTGGATGCCGTCCAGTTATTCTGGTTACCGCACTCTTTTACAAACTCATCGAACACCTCCCAAAAGCTGACAGGGGCTTCTTCCGGCTGTTCTTCACTGGTATCTTTCATTCTCATGTTGAAAGCTTCCTTCAACTGTTGGGTCGTTGGCATGACCTCCTGCACCTCAAATTCCTTGAAAATATTCTGGATTTCGGCATAGTATTTCAGCAAGTCCGTATTGATTTCGGCTGCACTTTGCTTTAGCTTGTTGGTACATCCGTTCTTTACCCGCTGCTTATCTGCATCCCATTTGGCTACGTCAATCCGGTAGCCCGTTGTAAACTCGATACGTTGGCTGGCAAAGATGACACGCATACGGATGGGTACGTTCTCTACGATTGGCACACCGTTCTTTTTCCGGCTCTCCAATGCAAAAATGATGTTGCGCTTGATATTCATAATTGGGTGCGTTTGAAATTCTACACCCAAATATACACCCAATTATTGAGATAGCAAAAGACATTTAGAAACATTTACTTTTACTCTATATTGTAATTTACACTTGATTATCAGTCGTTTGCAGTCTTATGATATTCTGTGAAAGTATAAGTTCGAGAGCCTGTCTCTCCGCAAAAAACGCTGAAAATCAGCAGATTGCAAAACAAACACCCTGTTTTACACCCAAGAATGTAAAGTCGGGTGTTTTTGTTTTATTTAAGATAATACAACCACTACATAATAAAAGAGTAGCGATATTAAAAGAATCCGATGAGAAAAGACTAATATTTATCTATCCATTCAGTTTGATTTCTCAGGACTTTACATCGTCCTGAAAGTATTTGTTGCCAGTGTTACAACCAATTAACCAATTCTGATTAGAAAAACTCATCGAGCATCAAATGAAACTGCAATTTATTCATATCAGGATTATCAATACCATATTTTTGAAAAAGCCGTTTCTGTAATGAAGGAGAAAACTCACCGAGGCAGTTCCATAGGATGGCAAGATCCTGGTATCGGTCTGCGATTCCGACTCGTCCAACATCAATACAACCTATTAATTTCCCCTCGTCAAAAATAAGGTTATCAAGTGAGAAATCACCATGAGTGACGACTGAATCCGGTGAGAATGGCAAAAGCTTATGCATTTCTTTCCAGACTTGTTCAACAGGCCAGCCATTACGCTCGTCATCAAAATCACTCGCATCAACCAAACCGTTATTCATTCGTGATTGCGCCTGAGCGAGGCGAAATACGCGATCGCTGTTAAAAGGACAATTACAAACAGGAATCGAATGCAACCGGCGCAGGAACACTGCCAGCGCATCAACAATATTTTCACCTGAATCAGGATATTCTTCTAATACCTGGAATGCTGTTTTCCCGGGGATCGCAGTGGTGAGTAACCATGCATCATCAGGAGTACGGATAAAATGCTTGATGGTCGGAAGAGGCATAAATTCCGTCAGCCAGTTTAGTCTGACCATCTCATCTGTAACATCATTGGCAACGCTACCTTTGCCATGTTTCAGAAACAACTCTGGCGCATCGGGCTTCCCATACAATCGATAGATTGTCGCACCTGATTGCCCGACATTATCGCGAGCCCATTTATACCCATATAAATCAGCATCCATGTTGGAATTTAATCGCGGCCTGGAGCAAGACGTTTCCCGTTGAATATGGCTCATAACACCCCTTGTATTACTGTTTATGTAAGCAGACAGTTTTATTGTTCATGATGATATATTTTTATCTTGTGCAATGTAACATCAGAGATTTTGAGACACAACGTGGCTTTGTTGAATAAATCGAACTTTTGCTGAGTTGAAGGATCAGCCGCGCAGTTCAACCTGTTGATAGTACGTACTAAGCTCTCATGTTTCACGTACTAAGCTCTCATGTTTAACGTACTAAGCTCTCATGTTTAACGAACTAAACCCTCATGGCTAACGTACTAAGCTCTCATGGCTAACGTACTAAGCTCTCATGTTTCACGTACTAAGCTCTCATGTTTGAACAATAAAATTAATATAAATCAGCAACTTAAATAGCCTCTAAGGTTTTAAGTTTTATAAGAAAAAAAAGAATATATAAGGCTTTTAAAGCTTTTAAGGTTTAACGGTTGTGGACAACAAGCCAGGGATGTAACGCACTGAGAAGCCCTTAGAGCCTCTCAAAGCAATTTTGAGTGACACAGGAACACTTAACGGCTGACATGGGGCGGCCGCTCAAGTAG
SEQ ID NO:76(pZR0901-Ec_frc,Of_oxc,Sc_YBR222C,Sc_YEL020C,At_AAE3,Ec_yfdE,Of_oxlT(中强度启动子)表达载体)：
GGAGAGCTGTATGTGGGTAGGATAAAACGAAAGGCTCAGTCGAAAGACTGGGCCTTTCGTTTTACAATTGGGCTACCTTTTTTTTGTTTTGTTTGCAATGGTTAATCTATTGTTAAAATTTAAAGTTTCACTTGAACTTTCAAATAATGTTCTTATATTTGCAGTGTCGAAAGAAACAAAGTAGCCTGGATCACACAACATTTAAAAAATAACATTATGAAAGCACTCGAAAAGAGATTCAAAGGTAATATTAACAATAATTTATTTTCAATGTCAACTCCACTTCAAGGAATTAAAGTTCTCGATTTCACCGGTGTGCAATCTGGCCCATCTTGTACTCAAATGCTGGCCTGGTTTGGCGCTGACGTTATTAAAATTGAACGTCCCGGCGTTGGTGACGTAACGCGTCACCAGCTGCGAGATATTCCTGATATCGATGCGCTTTACTTCACCATGCTTAACAGTAACAAACGTTCTATTGAGTTAAATACCAAAACAGCGGAAGGCAAAGAGGTAATGGAAAAGCTGATCCGCGAAGCTGATATCTTAGTCGAGAACTTTCATCCAGGGGCCATTGATCACATGGGCTTCACCTGGGAGCATATTCAAGAAATCAATCCACGTCTGATTTTTGGTTCGATCAAAGGGTTTGATGAGTGTTCGCCTTATGTGAATGTAAAAGCCTATGAAAACGTTGCTCAGGCAGCGGGTGGCGCGGCATCCACTACGGGTTTTTGGGATGGTCCGCCGCTGGTAAGCGCTGCAGCGTTGGGTGACAGCAACACCGGAATGCATTTGCTGATCGGTTTACTTGCTGCTTTGCTGCATCGCGAAAAAACGGGGCGTGGGCAACGAGTCACCATGTCAATGCAGGATGCCGTATTGAACCTTTGCCGCGTGAAATTACGTGACCAGCAGCGTCTAGATAAATTGGGTTATCTGGAAGAATACCCGCAGTATCCGAATGGTACATTTGGTGATGCAGTTCCCCGCGGTGGTAATGCAGGTGGTGGCGGTCAGCCTGGCTGGATCCTGAAATGTAAAGGCTGGGAAACCGATCCTAACGCCTATATTTATTTCACTATTCAGGAGCAAAACTGGGAAAACACCTGTAAAGCCATCGGCAAACCAGAATGGATTACCGATCCGGCATACAGTACAGCCCATGCACGACAGCCACATATTTTCGATATTTTTGCTGAAATCGAAAAATACACTGTCACTATTGATAAACATGAAGCGGTGGCCTATTTGACTCAGTTTGATATTCCTTGTGCACCGGTTTTAAGTATGAAAGAAATTTCACTTGATCCCTCTTTGCGCCAAAGTGGCAGTGTTGTTGAAGTGGAACAACCGTTGCGTGGAAAATATCTGACCGTTGGTTGTCCAATGAAATTCTCTGCCTTTACGCCGGATATTAAAGCTGCGCCGCTATTAGGTGAACATACCGCTGCTGTATTGCAGGAGCTGGGTTATAGCGACGATGAAATTGCTGCAATGAAGCAAAACCACGCCATCTGAGGTTCCTAGCTGATTAGAAGGCCATCCTGACGGATGGCCTTTTTTTTGACTGGTAAGCCAATCTAGGGCCTCGTCTGTAGGATAAAACGAAAGGCTCAGTCGAAAGACTGGGCCTTTCGTTTTACAATTGGGCTACCTTTTTTTTGTTTTGTTTGCAATGGTTAATCTATTGTTAAAATTTAAAGTTTCACTTGAACTTTCAAATAATGTTCTTATATTTGCAGTGTCGAAAGAAACAAAGTAGCCTGAGCTGTCACCGGATGTGCTTTCCGGTCTGATGAGTCCGTGAGGACGAAACAGCCTCTACAAATAATTTTGTTTAATCCATCAATTTAAAATTTAAAATAATGGTTTTTACTCTGGAAGATTTTGTTGGCGATTGGCGTCAGACCGCGGGTTATAATTTGGATCAAGTCCTGGAACAGGGTGGCGTAAGCTCTCTGTTCCAGAACCTGGGTGTGAGCGTGACGCCGATTCAGCGCATCGTTCTGTCCGGCGAGAACGGTCTGAAAATTGATATTCATGTGATCATCCCGTACGAAGGCCTGAGCGGTGACCAAATGGGTCAAATCGAGAAAATCTTTAAAGTCGTCTACCCAGTTGACGATCACCACTTCAAGGTTATCTTGCATTACGGTACGCTGGTGATTGATGGTGTGACCCCGAATATGATTGACTATTTCGGCCGTCCGTATGAAGGCATTGCCGTTTTTGACGGTAAAAAGATCACCGTCACCGGTACCCTGTGGAATGGCAATAAGATTATTGACGAGCGTCTGATTAACCCGGACGGCAGCCTGCTGTTCCGCGTGACCATCAACGGTGTCACGGGTTGGCGTCTGTGCGAGCGCATCCTGGCAGGTTCAATGAGTAACGACGACAATGTAGAGTTGACTGATGGCTTTCATGTTTTGATCGATGCCCTGAAAATGAATGACATCGATACCATGTATGGTGTTGTCGGCATTCCTATCACGAACCTGGCTCGTATGTGGCAAGATGACGGTCAGCGTTTTTACAGCTTCCGTCACGAACAACACGCAGGTTATGCAGCTTCTATCGCCGGTTACATCGAAGGAAAACCTGGCGTTTGCTTGACCGTTTCCGCCCCTGGCTTCCTGAACGGCGTGACTTCCCTGGCTCATGCAACCACCAACTGCTTCCCAATGATCCTGTTGAGCGGTTCCAGTGAACGTGAAATCGTCGATTTGCAACAGGGCGATTACGAAGAAATGGATCAGATGAATGTTGCACGTCCACACTGCAAAGCTTCTTTCCGTATCAACAGCATCAAAGACATTCCAATCGGTATCGCTCGTGCAGTTCGCACCGCTGTATCCGGACGTCCAGGTGGTGTTTACGTTGACTTGCCAGCAAAACTGTTCGGTCAGACCATTTCTGTAGAAGAAGCTAACAAACTGCTCTTCAAACCAATCGATCCAGCTCCGGCACAGATTCCTGCTGAAGATGCTATCGCTCGCGCTGCTGACCTGATCAAGAACGCCAAACGTCCAGTTATCATGCTGGGTAAAGGCGCTGCATACGCACAATGCGACGACGAAATCCGCGCACTGGTTGAAGAAACCGGCATCCCATTCCTGCCAATGGGTATGGCTAAAGGCCTGCTGCCTGACAACCATCCACAATCCGCTGCTGCAACCCGTGCTTTCGCACTGGCACAGTGTGACGTTTGCGTACTGATCGGCGCTCGTCTGAACTGGCTGATGCAGCACGGTAAAGGCAAAACCTGGGGCGACGAACTGAAGAAATACGTTCAGATCGACATCCAGGCTAACGAAATGGACAGCAACCAGCCTATCGCTGCACCAGTTGTTGGTGACATCAAGTCCGCCGTTTCCCTGCTCCGCAAAGCACTGAAAGGCGCTCCAAAAGCTGACGCTGAATGGACCGGCGCTCTGAAAGCCAAAGTTGACGGCAACAAAGCCAAACTGGCTGGCAAGATGACTGCCGAAACCCCATCCGGAATGATGAACTACTCCAATTCCCTGGGCGTTGTTCGTGACTTCATGCTGGCAAATCCGGATATTTCCCTGGTTAACGAAGGCGCTAATGCACTCGACAACACTCGTATGATTGTTGACATGCTGAAACCACGCAAACGTCTTGACTCCGGTACCTGGGGTGTTATGGGTATTGGTATGGGCTACTGCGTTGCTGCAGCTGCTGTTACCGGCAAACCGGTTATCGCTGTTGAAGGCGATAGCGCATTCGGTTTCTCCGGTATGGAACTGGAAACCATCTGCCGTTACAACCTGCCAGTTACCGTTATCATCATGAACAATGGTGGTATCTATAAAGGTAACGAAGCAGATCCACAACCAGGCGTTATCTCCTGTACCCGTCTGACCCGTGGTCGTTACGACATGATGATGGAAGCATTTGGCGGTAAAGGTTATGTTGCCAATACTCCAGCAGAACTGAAAGCTGCTCTGGAAGAAGCTGTTGCTTCCGGCAAACCATGCCTGATCAACGCGATGATCGATCCAGACGCTGGTGTCGAATCTGGCCGTATCAAGAGCCTGAACGTTGTAAGTAAAGTTGGCAAGAAATAAGGTTCCTAGCTGATTAGAAGGCCATCCTGACGGATGGCCTTTTTTTTGACTGCGTTCCTGGCGTGGTGCTCGCATAGTAGGATAAAACGAAAGGCTCAGTCGAAAGACTGGGCCTTTCGTTTTACAATTGGGCTACCTTTTTTTTGTTTTGTTTGCAATGGTTAATCTATTGTTAAAATTTAAAGTTTCACTTGAACTTTCAAATAATGTTCTTATATTTGCAGTGTCGAAAGAAACAAAGTAGCCTGGATCACACAACATTTAAAAAATAACATTATGAAAGCACTCGAAAAGAGATTCAAAGGTAATATTAACAATAATTTATTTTCAATGACAAGTGCCGCTACTGTTACTGCTTCGTTCAACGATACTTTTAGCGTATCCGATAATGTCGCCGTTATTGTTCCTGAAACGGACACTCAGGTGACCTACAGGGATCTATCCCACATGGTGGGTCACTTCCAGACCATGTTCACAAATCCTAATTCTCCATTGTACGGAGCTGTTTTCAGACAAGATACAGTGGCGATATCCATGCGTAATGGGCTGGAATTTATCGTCGCTTTCCTCGGTGCTACTATGGACGCTAAAATTGGCGCGCCCTTGAATCCCAATTATAAGGAAAAGGAGTTCAATTTTTATTTGAATGACCTGAAATCTAAGGCGATTTGCGTCCCAAAGGGTACCACAAAGTTACAGAGTTCTGAAATTCTAAAATCTGCCTCCACGTTTGGATGTTTTATCGTAGAGCTGGCCTTCGATGCGACCAGGTTTAGGGTAGAGTATGATATATACTCTCCAGAGGACAACTACAAAAGGGTTATTTACCGGTCTTTGAACAACGCCAAATTTGTCAACACAAATCCCGTTAAATTCCCTGGGTTTGCCCGTTCCAGTGACGTTGCCCTGATTTTGCATACCAGTGGTACCACCTCCACTCCAAAAACGGTGCCTTTGTTACATTTGAACATTGTGAGAAGCACGTTGAACATTGCTAACACTTACAAGCTAACGCCCTTGGACAGATCTTATGTCGTGATGCCTCTTTTCCACGTCCATGGGTTAATTGGTGTTTTACTTTCCACTTTTAGAACTCAGGGTTCTGTTGTGGTTCCCGATGGATTCCATCCAAAGTTATTCTGGGACCAATTTGTTAAGTACAACTGTAATTGGTTCAGTTGCGTTCCCACAATAAGCATGATTATGCTGAACATGCCCAAACCAAACCCTTTCCCACACATTAGATTCATCAGATCGTGTTCTTCTGCTTTGGCTCCAGCAACGTTCCATAAGCTGGAGAAGGAATTCAATGCACCTGTCTTGGAGGCCTATGCGATGACCGAAGCATCACATCAAATGACCTCAAACAATCTGCCTCCAGGAAAGAGAAAGCCTGGTACTGTGGGCCAGCCACAAGGAGTCACCGTCGTCATTCTAGATGACAATGACAATGTCTTGCCCCCGGGCAAAGTCGGCGAAGTTTCCATCAGAGGCGAAAACGTCACTTTGGGGTATGCTAATAATCCAAAAGCTAACAAGGAGAACTTCACCAAGAGAGAGAACTATTTCAGAACCGGTGACCAAGGTTATTTCGACCCTGAGGGGTTTTTGGTCCTTACAGGCAGAATCAAAGAGCTTATCAACAGGGGTGGTGAAAAGATTTCACCCATTGAGCTCGACGGCATTATGCTATCGCATCCAAAGATCGATGAAGCCGTTGCATTTGGTGTTCCCGACGATATGTACGGCCAAGTAGTTCAAGCCGCCATTGTTTTGAAGAAGGGAGAAAAAATGACCTACGAAGAACTGGTGAACTTCTTAAAGAAGCACCTAGCCTCTTTCAAAATTCCAACCAAGGTGTACTTTGTTGATAAGCTACCAAAAACCGCTACAGGTAAAATCCAGAGAAGAGTTATCGCAGAAACTTTTGCTAAGAGCAGCAGAAATAAGAGTAAGTTGTAGGGTTCCTAGCTGATTAGAAGGCCATCCTGACGGATGGCCTTTTTTTTGACTGCCCGACGAACGGACTAATCCTGACGTAGGATAAAACGAAAGGCTCAGTCGAAAGACTGGGCCTTTCGTTTTACAATTGGGCTACCTTTTTTTTGTTTTGTTTGCAATGGTTAATCTATTGTTAAAATTTAAAGTTTCACTTGAACTTTCAAATAATGTTCTTATATTTGCAGTGTCGAAAGAAACAAAGTAGCCTGGATCACACAACATTTAAAAAATAACATTATGAAAGCACTCGAAAAGAGATTCAAAGGTAATATTAACAATAATTTATTTTCAATGACCACGACCGCTACCCAACACTTCGCTCAACTTTTACAAAAATATGGCATCGACACAGTCTTTGGAATTGTGGGCATCCCTATTGTCCAACTAGCGGATACGATGGTTGCCAACGGTATCAAATTCATTCCATGCAGGAACGAACAGGCTGCCTCATATGCAGCCTCTGCTTATGGTTATATTAGTGATAAGCCCGGCGTGTTACTTATTGTCGGGGGCCCCGGTTTAATCCACGCACTGGCTGGTATATACAACTCAATGAGTAATAGGTGGCCTCTTCTAGTAATTGCAGGGAGTTCTTCTCAAAGTGATATACATAAAGGCGGGTTTCAAGAGTTGGATCAAGTTAGTCTTTTGTCCCCATTTTTGAAGTTTACCGGAAAGTTAACCCCTGACAATATTGACATGATTACTCAGAAGGCTTTGAATTACTGTATACAAGGCACTGCAGGGGTTTCTTACATAGACGTCCCTGCAGATTTTATTGAGTATGAAAAACCCTTAGAAGGAAACGACCGTACAGGAAATGAATTACCGATGATATTAACTCCAAACATATGTGGCCCTGATCCGTCGAAGATCAAAAAAGTCGTACAGCTTATTTTGCAACATAAAAATAAAAATATTCTGATTGTTATTGGAAAGGGTGCGGTAAAAAACTCACACGAAATCCGCAGACTAGTAAATACGTTCAATCTACCATTTTTACCCACCCCAATGGCTAAGGGAATTGTCCCAGACTCTTCTCCACTGAACGTTTCATCTGCAAGATCTCAAGCATTGAAAATAGCGGATATTGTTCTTGTTCTCGGGGCAAGATTAAACTGGATATTGCATTTTGGTACTTCACCCAAATGGAACTCAGAATCCATATTCATCCAGTTCGATTCCAATCCCGAAACCTTGGGGGATAACAATGTCTCACCTGGTGCTGACCTTTCCATATGGGGAGATATAGGCTTGAGCGTAACCGCTTTAGTTGAGGAATTGACTCGCCAAGATTCCTGCTGGAAGTATAGTGGTGTTAAGCAAGAAATACGAGAGAAAATTCAACTGAATCAAACTCGTCTATTGAGGAAAGAGAAGACTAGAGGGGCACAATTGAATTACAACCAGGTTTATGGAACATTGAGGCCTCTTATCGATGATTACAGGACAATACTTGTGACGGAGGGAGCAAATACTATGGATATTGCGCGTATTTCATTCCCTACAGACGCTCCAAGGCGCCGTTTAGACGCAGGGACCAACGCGACTATGGGGATTGGGCTCGGATATGCTCTTGCATGCAAGGCATCTCATCCGGAACTCGATGTGGTGCTGATTCAGGGTGATTCCGCATTTGGATTCTCTGCCATGGAAATTGAAACGGCAGTAAGGTGTCAGCTGGCATTGGTCATCGTTGTGATGAACAATAGCGGTATTTACCATGGAGAAAAGGATATAGAGGGTGATTTACCTCCAACGGCACTAAGCAAGAACTGCCGTTACGATCTCGTAGGCAAAGGACTGGGTGCCAATGATTTTTTTGTCAACACAATAAGCGAACTGAGCAGATGCTTCCAACAAGCCGTGCAGCTTTCTCGGACTAAAAGAGAAACAAGTGTCATCAACGTTATCATTGAGCCTGGCGAACAAAAGCAAATTGCCTTTGCCTGGCAGAATAAACCGCGTTTATAGGGTTCCTAGCTGATTAGAAGGCCATCCTGACGGATGGCCTTTTTTTTGACTGCTCTGGGTCTGACGGCCATCAAGGGTAGGATAAAACGAAAGGCTCAGTCGAAAGACTGGGCCTTTCGTTTTACAATTGGGCTACCTTTTTTTTGTTTTGTTTGCAATGGTTAATCTATTGTTAAAATTTAAAGTTTCACTTGAACTTTCAAATAATGTTCTTATATTTGCAGTGTCGAAAGAAACAAAGTAGCCTGGATCACACAACATTTAAAAAATAACATTATGAAAGCACTCGAAAAGAGATTCAAAGGTAATATTAACAATAATTTATTTTCAATGGATAGCGATACTCTCTCAGGATTATTGGAAAACGTCGCCAAAAAATTCCCCGATCGCCGAGCTCTCTCCGTTTCTGGAAAATTCAATCTCACTCACGCGCGTCTTCACGATCTAATCGAACGCGCCGCTTCACGCCTTGTCTCCGACGCTGGAATCAAACCCGGCGATGTCGTTGCTCTCACCTTCCCTAACACCGTCGAGTTTGTTATAATGTTTTTAGCGGTGATAAGAGCTAGAGCCACGGCGGCGCCGTTGAACGCAGCGTACACGGCGGAGGAATTTGAGTTTTACCTCTCCGATTCAGATTCAAAGCTATTGTTAACCTCTAAAGAAGGAAACGCACCGGCTCAAGAAGCAGCTTCAAAGCTGAAAATCTCTCACGTCACCGCTACGCTGCTTGACGCTGGCTCGGACCTTGTACTATCCGTTGCGGATTCAGATTCCGTCGTTGACTCAGCGACGGAACTCGTTAATCACCCGGACGACGGTGCTCTCTTCCTCCACACTTCTGGCACTACGAGCCGTCCAAAGGGTGTACCGCTTACGCAGCTCAATCTAGCTTCATCCGTCAAGAACATTAAAGCTGTGTACAAGCTTACTGAGTCTGATTCTACGGTGATTGTTCTCCCTCTGTTCCATGTTCATGGATTGTTAGCTGGGTTGCTTAGCTCGCTTGGAGCTGGTGCTGCTGTAACTCTTCCAGCTGCTGGTAGATTCTCAGCAACAACATTTTGGCCAGATATGAAGAAGTATAACGCTACATGGTATACTGCTGTGCCGACCATTCATCAGATCATATTGGACCGCCACGCGAGCCACCCTGAAACGGAATATCCTAAACTCCGGTTCATCAGGAGTTGCAGTGCTTCTTTGGCTCCGGTGATATTGTCCAGGCTTGAGGAAGCGTTTGGAGCACCGGTGCTCGAGGCCTATGCAATGACAGAGGCAACACATTTGATGAGCTCAAACCCCTTACCAGAGGAAGGTCCACACAAGCCTGGGTCTGTTGGGAAACCGGTAGGTCAAGAAATGGCGATCCTTAATGAGAAAGGCGAGATCCAAGAGCCAAATAACAAAGGAGAGGTTTGTATAAGAGGTCCAAATGTGACCAAGGGTTACAAGAATAACCCAGAAGCCAACAAGGCAGGTTTCGAGTTTGGGTGGTTCCACACTGGTGATATCGGTTACTTTGATACCGATGGGTATTTGCATCTGGTGGGTCGGATCAAAGAGCTTATTAACCGTGGAGGTGAGAAGATATCTCCAATTGAAGTGGATGCAGTACTCTTAACGCATCCTGACGTTTCTCAGGGTGTTGCATTCGGTGTTCCTGATGAGAAATATGGGGAAGAGATTAACTGTGCGGTGATTCCAAGAGAAGGAACTACTGTAACCGAAGAGGACATTAAAGCGTTTTGTAAGAAGAATTTGGCAGCTTTCAAGGTGCCAAAGAGAGTGTTCATCACTGATAACCTCCCCAAAACTGCCTCTGGTAAGATTCAGCGCCGTATCGTCGCACAACATTTCCTTGAGAAGCCCTGAGGTTCCTAGCTGATTAGAAGGCCATCCTGACGGATGGCCTTTTTTTTGACTGGGTGTTCCGATACACTCAATTTGTGTAGGATAAAACGAAAGGCTCAGTCGAAAGACTGGGCCTTTCGTTTTACAATTGGGCTACCTTTTTTTTGTTTTGTTTGCAATGGTTAATCTATTGTTAAAATTTAAAGTTTCACTTGAACTTTCAAATAATGTTCTTATATTTGCAGTGTCGAAAGAAACAAAGTAGCCTGGATCACACAACATTTAAAAAATAACATTATGAAAGCACTCGAAAAGAGATTCAAAGGTAATATTAACAATAATTTATTTTCAATGACAAATAATGAAAGCAAAGGGCCGTTTGAAGGCTTATTAGTTATCGATATGACACATGTCCTTAATGGACCTTTCGGAACTCAACTTCTTTGTAATATGGGCGCAAGGGTAATTAAAGTTGAGCCGCCGGGTCATGGTGATGATACCCGCACATTTGGTCCCTATGTGGATGGACAGTCACTCTATTACAGTTTTATTAATCATGGCAAAGAGAGTGTGGTTCTTGATTTAAAGAATGATCACGATAAAAGTATATTTATAAATATGCTCAAACAAGCTGATGTATTAGCTGAGAATTTTCGCCCAGGTACAATGGAAAAACTGGGGTTTTCATGGGAAACGCTTCAAGAAATCAACCCGCGCCTCATATATGCTTCATCGTCAGGTTTCGGACATACCGGTCCGCTAAAAGATGCTCCTGCCTACGATACCATCATTCAGGCAATGAGCGGGATAATGATGGAAACAGGATATCCTGATGCTCCGCCAGTGCGCGTTGGTACATCTCTTGCGGATCTATGCGGCGGTGTCTATTTATTCAGCGGAATAGTGAGTGCACTTTATGGCCGCGAAAAGAGCCAGAGAGGGGCGCATGTCGATATAGCGATGTTTGATGCCACGCTGAGTTTTCTGGAGCATGGTCTGATGGCATATATCGCAACTGGGAAGTCACCACAACGTCTGGGAAATCGCCATCCCTACATGGCACCTTTTGATGTTTTCAATACTCAGGATAAGCCGATTACGATTTGTTGTGGTAATGACAAGCTTTTTTCTGCGTTATGCCAGGCACTGGAGCTTACGGAACTGGTTAATGATCCCCGATTTAGCAGCAATATTTTACGCGTACAAAACCAGGCTATTCTTAAACAATATATTGAGCGGACGTTAAAAACGCAGGCAGCTGAAGTTTGGTTAGCCAGAATACATGAAGTTGGTGTACCCGTCGCGCCGTTATTAAGTGTGGCTGAGGCCATTAAATTGCCACAAACTCAGGCGAGAAATATGTTGATTGAAGCCGGGGGAATAATGATGCCGGGTAATCCGATAAAAATCAGCGGCTGCGCGGACCCGCATGTTATGCCGGGAGCGGCAACGCTCGACCAGCATGGGGAACAAATTCGCCAGGAGTTCTCATCATAAGGTTCCTAGCTGATTAGAAGGCCATCCTGACGGATGGCCTTTTTTTTGACTTAGTTTCAATTTTACATTCCAGGAAGTAGGATAAAACGAAAGGCTCAGTCGAAAGACTGGGCCTTTCGTTTTACAATTGGGCTACCTTTTTTTTGTTTTGTTTGCAATGGTTAATCTATTGTTAAAATTTAAAGTTTCACTTGAACTTTCAAATAATGTTCTTATATTTCCAGTGTCGAAAGAAACAAAGTAGCCTGGATCACACAACATTTAAAAAATAACATTATGAAAGCACTCGAAAAGAGATTCAAAGGTAATATTAACAATAATTTATTTTAATGAATAATCCACAAACAGGACAATCAACAGGCCTCTTGGGCAATCGTTGGTTCTACTTGGTATTAGCAGTTTTGCTGATGTGTATGATCTCGGGTGTCCAATATTCCTGGACACTGTACGCTAACCCGGTTAAAGACAACCTTGGCGTTTCTTTGGCTGCGGTTCAGACGGCTTTCACACTCTCTCAGGTCATTCAAGCTGGTTCTCAGCCTGGTGGTGGTTACTTCGTTGATAAATTCGGTCCAAGAATTCCATTGATGTTCGGTGGTGCGATGGTTCTCGCTGGCTGGACCTTCATGGGTATGGTTGACAGTGTTCCTGCTCTGTATGCTCTTTATACTCTGGCCGGTGCAGGTGTTGGTATCGTTTACGGTATCGCGATGAACACGGCTAACAGATGGTTCCCGGACAAACGCGGTCTGGCTTCCGGTTTCACCGCTGCCGGTTACGGTCTGGGTGTTCTGCCGTTCCTGCCACTGATCAGCTCCGTTCTGAAAGTTGAAGGTGTTGGCGCAGCATTCATGTACACCGGTTTGATCATGGGTATCCTGATTATCCTGATCGCTTTCGTTATCCGTTTCCCTGGCCAGCAAGGCGCCAAAAAACAAATCGTTGTTACCGACAAGGATTTCAATTCTGGCGAAATGCTGAGAACACCACAATTCTGGGTTCTGTGGACCGCATTCTTTTCCGTTAACTTTGGTGGTTTGCTGCTGGTTGCCAACAGCGTCCCTTACGGTCGCAGCCTCGGTCTTGCCGCAGGTGTGCTGACGATCGGTGTTTCGATCCAGAACCTGTTCAATGGTGGTTGCCGTCCTTTCTGGGGTTTCGTTTCCGATAAAATCGGCCGTTACAAAACCATGTCCGTCGTTTTCGGTATCAATGCTGTTGTTCTCGCACTTTTCCCGACGATTGCTGCCTTGGGCGATGTAGCCTTTATCGCCATGTTGGCAATCGCATTCTTCACATGGGGTGGTAGCTACGCTCTGTTCCCATCGACCAACAGCGATATTTTCGGTACGGCATACTCTGCCAGAAACTATGGTTTCTTCTGGGCTGCAAAAGCAACTGCCTCGATCTTCGGTGGTGGTCTGGGTGCTGCAATTGCAACCAACTTCGGATGGAATACCGCTTTCCTGATTACTGCGATTACTTCTTTCATCGCATTTGCTCTGGCTACCTTCGTTATTCCAAGAATGGGCCGTCCAGTCAAGAAAATGGTCAAATTGTCTCCAGAAGAAAAAGCTGTACATTAAGGTTCCTAGCTGATTAGAAGGCCATCCTGACGGATGGCCTTTTTTTTGACTGCTATGACTAAACGGCGCGCCTGATAGGTGGGCTGCCCTTCCTGGTTGGCTTGGTTTCATCAGCCATCCGCTTGCCCTCATCTGTTACGCCGGCGGTAGCCGGCCAGCCTCGCAGAGCAGGATTCCCGTTGAGCACCGCCAGGTGCGAATAAGGGACAGTGAAGAAGGAACACCCGCTCGCGGGTGGGCCTACTTCACCTATCCTGCCCGGCTGACGCCGTTGGATACACCAAGGAAAGTCTACACGAACCCTTTGGCAAAATCCTGTATATCGTGCGAAAAAGGATGGATATACCGAAAAAATCGCTATAATGACCCCGAAGCAGGGTTATGCAGCGGAAAAGTTATATACATTCATGTCCATTTATGTAAAAAATCCTGCTGACCTTGTTTATGTCTTGTCAGTCACCATTTGCAAAACCATATTTGACCCTCAAAGAGGCTGAATTTGATAAGCAACTTGCTACATACTCATAATAAGGAGCTAAATAGAACACGAATGGGAAATACTCAAATGCCAAACTAAAGAAGATATTGGCCAAAATAAACGCTATACCGAGAGAGAAACTTGATTTTTCAACTTCCTAAAACAGTGTTGTTCAAACATTTCTACTTATTTGTACTTACCAGTTGAACCTACGTTTCCCTAATAAAATGTCTATGGTAAAAAGTTAAAAAATCCTCCTACTTTTGTTAGATATATTTTTTTGTGTAATTTTGTAATCGTTATGCGGCAGTAATAATATACATATTAATACGAGTTAGGAATCCTGTAGTTCTCATATGCTACGAGGAGGTATTAAAAGGTGCGTTTCGACAATGCATCTATTGTAGTATATTATTGCTTAATCCAAATGAATATTATAAATTTAGGAATTCTTGCTCACATTGATGCAGGAAAAACTTCCGTAACCGAGAATCTGCTGTTTGCCAGTGGAGCAACGGAAAAGTGCGGCTGTGTGGATAATGGTGACACCATAACGGACTCTATGGATATAGAGAAACGTAGAGGAATTACTGTTCGGGCTTCTACGACATCTATTATCTGGAATGGTGTGAAATGCAATATCATTGACACTCCGGGACACATGGATTTTATTGCGGAAGTGGAGCGGACATTCAAAATGCTTGATGGAGCAGTCCTCATCTTATCCGCAAAGGAAGGCATACAAGCGCAGACAAAGTTGCTGTTCAATACTTTACAGAAGCTGCAAATCCCGACAATTATATTTATCAATAAGATTGACCGAGCCGGTGTGAATTTGGAGCGTTTGTATCTGGATATAAAAGCAAATCTGTCTCAAGATGTCCTGTTTATGCAAAATGTTGTCGATGGATCGGTTTATCCGGTTTGCTCCCAAACATATATAAAGGAAGAATACAAAGAATTTGTATGCAACCATGACGACAATATATTAGAACGATATTTGGCGGATAGCGAAATTTCACCGGCTGATTATTGGAATACGATAATCGCTCTTGTGGCAAAAGCCAAAGTCTATCCGGTGCTACATGGATCAGCAATGTTCAATATCGGTATCAATGAGTTGTTGGACGCCATCACTTCTTTTATACTTCCTCCGGCATCGGTTTCAAACAGACTTTCATCTTATCTTTATAAGATAGAGCATGACCCCAAAGGACATAAAAGAAGTTTTCTAAAAATAATTGACGGAAGTCTGAGACTTCGAGATGTTGTAAGAATCAACGATTCGGAAAAATTCATCAAGATTAAAAATCTAAAAACTATCAATCAGGGCAGAGAGATAAATGTTGATGAAGTGGGCGCCAATGATATCGCGATTGTAGAGGATATGGATGATTTTCGAATCGGAAATTATTTAGGTGCTGAACCTTGTTTGATTCAAGGATTATCGCATCAGCATCCCGCTCTCAAATCCTCCGTCCGGCCAGACAGGCCCGAAGAGAGAAGCAAGGTGATATCCGCTCTGAATACATTGTGGATTGAAGATCCGTCTTTGTCCTTTTCCATAAACTCATATAGTGATGAATTGGAAATCTCGTTATATGGTTTAACCCAAAAGGAAATCATACAGACATTGCTGGAAGAACGATTTTCCGTAAAGGTCCATTTTGATGAGATCAAGACTATATACAAAGAACGACCTGTAAAAAAGGTCAATAAGATTATTCAGATCGAAGTGCCGCCCAACCCTTATTGGGCCACAATAGGGCTGACTCTTGAACCCTTACCGTTAGGGACAGGGTTGCAAATCGAAAGTGACATCTCCTATGGTTATCTGAACCATTCTTTTCAAAATGCCGTTTTTGAAGGGATTCGTATGTCTTGCCAATCCGGGTTACATGGATGGGAAGTGACTGATCTGAAAGTAACTTTTACTCAAGCCGAGTATTATAGCCCGGTAAGTACACCAGCTGATTTCAGACAGCTGACCCCTTATGTCTTTAGGCTGGCCTTGCAACAGTCAGGTGTGGACATTCTCGAACCGATGCTCTATTTTGAGTTGCAGATACCCCAAGCGGCAAGTTCCAAAGCTATTACAGATTTGCAAAAAATGATGTCTGAGATTGAAGATATCAGTTGCAATAATGAGTGGTGTCATATTAAAGGGAAAGTTCCATTAAATACAAGTAAAGACTATGCATCAGAAGTAAGTTCATACACTAAGGGCTTAGGCATTTTTATGGTTAAGCCATGCGGGTATCAAATAACAAAAGGCGGTTATTCTGATAATATCCGCATGAACGAAAAAGATAAACTTTTATTCATGTTCCAAAAATCAATGTCATCAAAATAACCACGAGTCATTGGTAACTATCTATGAAACTGTTTGATACTTTTATAGTTGATTAAACTTGTTCATGGCATTTGCCTTAATATCATCCGCTATGTCAATGTAGGGTTTCATAGCTTTGTAGTCGCTGTGTCCCGTCCATTTCATGACCACCTGTGCCGGGATTCCGAGAGCCAGCGCATTGCAGATGAATGTCCTTTTTCCTGCATGGGTACTGAGCAAAGCGTATTTGGGTGTGACTTCATCAATACGTTCATTTCCCTTGTAGTAGGTTTCCCGTACAGGCTCGTTGATTTCTGCCAGTTCGCCCAGCTCTTTCAGGTAATCGTTCATCTTCTGGTTGCTGATGACGGGCAGAGCCATGTAATTCTCGAAATGGATGTCCTTGTATTTGTCCAGTATGGCTTTGCTGTATTTGTTCAGTTCAATCGTCAGGCTGTCGGCAGTCTTGACTGTGGTTATTTCGATGTGGTCGGACTTCACATCGCTTCTTTTCAGATTGCGAACATCCGAATACCGCAAACTCGTAAAGCAGCAGAACAGGAAAACATCACGCACACGTTCCAGGTATTGCTTATCCTTGGGTATCTGGTAGTCTTTCAGCTTGTTCAGTTCATCCCAAGTCAGGAAGATTACTTTTTTCGAGGTGGTTTTCAGTTTCGGTTTGAACGTATCGTATGCAATGTTCTGATGATGTCCTTTCTTGAAGCTCCAGCGCAGGAACCATTTGAGGAATCCCATTTGCTTGCCGATGGTGCTGTTTCTCATATCCTTGGTGTCACGCAGGAAGTTGACGTATTCGTTCAATCCAAACTCGTTGAAATAGTTGAACGTTGCATCCTCCTTGAACTCTTTGAGGTGGTTCCTCACTGCTGCAAATTTTTCATAGGTGGATGCCGTCCAGTTATTCTGGTTACCGCACTCTTTTACAAACTCATCGAACACCTCCCAAAAGCTGACAGGGGCTTCTTCCGGCTGTTCTTCACTGGTATCTTTCATTCTCATGTTGAAAGCTTCCTTCAACTGTTGGGTCGTTGGCATGACCTCCTGCACCTCAAATTCCTTGAAAATATTCTGGATTTCGGCATAGTATTTCAGCAAGTCCGTATTGATTTCGGCTGCACTTTGCTTTAGCTTGTTGGTACATCCGTTCTTTACCCGCTGCTTATCTGCATCCCATTTGGCTACGTCAATCCGGTAGCCCGTTGTAAACTCGATACGTTGGCTGGCAAAGATGACACGCATACGGATGGGTACGTTCTCTACGATTGGCACACCGTTCTTTTTCCGGCTCTCCAATGCAAAAATGATGTTGCGCTTGATATTCATAATTGGGTGCGTTTGAAATTCTACACCCAAATATACACCCAATTATTGAGATAGCAAAAGACATTTAGAAACATTTACTTTTACTCTATATTGTAATTTACACTTGATTATCAGTCGTTTGCAGTCTTATGATATTCTGTGAAAGTATAAGTTCGAGAGCCTGTCTCTCCGCAAAAAACGCTGAAAATCAGCAGATTGCAAAACAAACACCCTGTTTTACACCCAAGAATGTAAAGTCGGGTGTTTTTGTTTTATTTAAGATAATACAACCACTACATAATAAAAGAGTAGCGATATTAAAAGAATCCGATGAGAAAAGACTAATATTTATCTATCCATTCAGTTTGATTTCTCAGGACTTTACATCGTCCTGAAAGTATTTGTTGCCAGTGTTACAACCAATTAACCAATTCTGATTAGAAAAACTCATCGAGCATCAAATGAAACTGCAATTTATTCATATCAGGATTATCAATACCATATTTTTGAAAAAGCCGTTTCTGTAATGAAGGAGAAAACTCACCGAGGCAGTTCCATAGGATGGCAAGATCCTGGTATCGGTCTGCGATTCCGACTCGTCCAACATCAATACAACCTATTAATTTCCCCTCGTCAAAAATAAGGTTATCAAGTGAGAAATCACCATGAGTGACGACTGAATCCGGTGAGAATGGCAAAAGCTTATGCATTTCTTTCCAGACTTGTTCAACAGGCCAGCCATTACGCTCGTCATCAAAATCACTCGCATCAACCAAACCGTTATTCATTCGTGATTGCGCCTGAGCGAGGCGAAATACGCGATCGCTGTTAAAAGGACAATTACAAACAGGAATCGAATGCAACCGGCGCAGGAACACTGCCAGCGCATCAACAATATTTTCACCTGAATCAGGATATTCTTCTAATACCTGGAATGCTGTTTTCCCGGGGATCGCAGTGGTGAGTAACCATGCATCATCAGGAGTACGGATAAAATGCTTGATGGTCGGAAGAGGCATAAATTCCGTCAGCCAGTTTAGTCTGACCATCTCATCTGTAACATCATTGGCAACGCTACCTTTGCCATGTTTCAGAAACAACTCTGGCGCATCGGGCTTCCCATACAATCGATAGATTGTCGCACCTGATTGCCCGACATTATCGCGAGCCCATTTATACCCATATAAATCAGCATCCATGTTGGAATTTAATCGCGGCCTGGAGCAAGACGTTTCCCGTTGAATATGGCTCATAACACCCCTTGTATTACTGTTTATGTAAGCAGACAGTTTTATTGTTCATGATGATATATTTTTATCTTGTGCAATGTAACATCAGAGATTTTGAGACACAACGTGGCTTTGTTGAATAAATCGAACTTTTGCTGAGTTGAAGGATCAGCCGCGCAGTTCAACCTGTTGATAGTACGTACTAAGCTCTCATGTTTCACGTACTAAGCTCTCATGTTTAACGTACTAAGCTCTCATGTTTAACGAACTAAACCCTCATGGCTAACGTACTAAGCTCTCATGGCTAACGTACTAAGCTCTCATGTTTCACGTACTAAGCTCTCATGTTTGAACAATAAAATTAATATAAATCAGCAACTTAAATAGCCTCTAAGGTTTTAAGTTTTATAAGAAAAAAAAGAATATATAAGGCTTTTAAAGCTTTTAAGGTTTAACGGTTGTGGACAACAAGCCAGGGATGTAACGCACTGAGAAGCCCTTAGAGCCTCTCAAAGCAATTTTGAGTGACACAGGAACACTTAACGGCTGACATGGGGCGGCCGCTCAAGTAG
SEQ ID NO:77(pZR1871-Ec_frc,Of_oxc,Sc_YBR222C,Sc_YEL020C,At_AAE3,Ec_yfdE,Of_oxlT(高强度启动子)表达载体)：
GGAGAGCTGTATGTGGGTAGGATAAAACGAAAGGCTCAGTCGAAAGACTGGGCCTTTCGTTTTACAATTGGGCTACCTTTTTTTTGTTTTGTTTGCAATGGTTAATCTATTGTTAAAATTTAAAGTTTCACTTGAACTTTCAAATAATGTTCTTATATTTGCAGTGTCGAAAGAAACAAAGTAGCCTGGATCACACAACATTTAAAAAATAACATTATGAAAGCACTCGAAAAGAGATTCAAAGGTAATATTAACAATAATTTATTTTCAATGTCAACTCCACTTCAAGGAATTAAAGTTCTCGATTTCACCGGTGTGCAATCTGGCCCATCTTGTACTCAAATGCTGGCCTGGTTTGGCGCTGACGTTATTAAAATTGAACGTCCCGGCGTTGGTGACGTAACGCGTCACCAGCTGCGAGATATTCCTGATATCGATGCGCTTTACTTCACCATGCTTAACAGTAACAAACGTTCTATTGAGTTAAATACCAAAACAGCGGAAGGCAAAGAGGTAATGGAAAAGCTGATCCGCGAAGCTGATATCTTAGTCGAGAACTTTCATCCAGGGGCCATTGATCACATGGGCTTCACCTGGGAGCATATTCAAGAAATCAATCCACGTCTGATTTTTGGTTCGATCAAAGGGTTTGATGAGTGTTCGCCTTATGTGAATGTAAAAGCCTATGAAAACGTTGCTCAGGCAGCGGGTGGCGCGGCATCCACTACGGGTTTTTGGGATGGTCCGCCGCTGGTAAGCGCTGCAGCGTTGGGTGACAGCAACACCGGAATGCATTTGCTGATCGGTTTACTTGCTGCTTTGCTGCATCGCGAAAAAACGGGGCGTGGGCAACGAGTCACCATGTCAATGCAGGATGCCGTATTGAACCTTTGCCGCGTGAAATTACGTGACCAGCAGCGTCTAGATAAATTGGGTTATCTGGAAGAATACCCGCAGTATCCGAATGGTACATTTGGTGATGCAGTTCCCCGCGGTGGTAATGCAGGTGGTGGCGGTCAGCCTGGCTGGATCCTGAAATGTAAAGGCTGGGAAACCGATCCTAACGCCTATATTTATTTCACTATTCAGGAGCAAAACTGGGAAAACACCTGTAAAGCCATCGGCAAACCAGAATGGATTACCGATCCGGCATACAGTACAGCCCATGCACGACAGCCACATATTTTCGATATTTTTGCTGAAATCGAAAAATACACTGTCACTATTGATAAACATGAAGCGGTGGCCTATTTGACTCAGTTTGATATTCCTTGTGCACCGGTTTTAAGTATGAAAGAAATTTCACTTGATCCCTCTTTGCGCCAAAGTGGCAGTGTTGTTGAAGTGGAACAACCGTTGCGTGGAAAATATCTGACCGTTGGTTGTCCAATGAAATTCTCTGCCTTTACGCCGGATATTAAAGCTGCGCCGCTATTAGGTGAACATACCGCTGCTGTATTGCAGGAGCTGGGTTATAGCGACGATGAAATTGCTGCAATGAAGCAAAACCACGCCATCTGAGGTTCCTAGCTGATTAGAAGGCCATCCTGACGGATGGCCTTTTTTTTGACTGGTAAGCCAATCTAGGGCCTCGTCTGTAGGATAAAACGAAAGGCTCAGTCGAAAGACTGGGCCTTTCGTTTTACAATTGGGCTACCTTTTTTTTGTTTTGTTTGCAATGGTTAATCTATTGTTAAAATTTAAAGTTTCACTTGAACTTTCAAATAATGTTCTTATATTTGCAGTGTCGAAAGAAACAAAGTAGCCTGAGCTGTCACCGGATGTGCTTTCCGGTCTGATGAGTCCGTGAGGACGAAACAGCCTCTACAAATAATTTTGTTTAATCCATCAATTTAAAATTTAAAATAATGGTTTTTACTCTGGAAGATTTTGTTGGCGATTGGCGTCAGACCGCGGGTTATAATTTGGATCAAGTCCTGGAACAGGGTGGCGTAAGCTCTCTGTTCCAGAACCTGGGTGTGAGCGTGACGCCGATTCAGCGCATCGTTCTGTCCGGCGAGAACGGTCTGAAAATTGATATTCATGTGATCATCCCGTACGAAGGCCTGAGCGGTGACCAAATGGGTCAAATCGAGAAAATCTTTAAAGTCGTCTACCCAGTTGACGATCACCACTTCAAGGTTATCTTGCATTACGGTACGCTGGTGATTGATGGTGTGACCCCGAATATGATTGACTATTTCGGCCGTCCGTATGAAGGCATTGCCGTTTTTGACGGTAAAAAGATCACCGTCACCGGTACCCTGTGGAATGGCAATAAGATTATTGACGAGCGTCTGATTAACCCGGACGGCAGCCTGCTGTTCCGCGTGACCATCAACGGTGTCACGGGTTGGCGTCTGTGCGAGCGCATCCTGGCAGGTTCAATGAGTAACGACGACAATGTAGAGTTGACTGATGGCTTTCATGTTTTGATCGATGCCCTGAAAATGAATGACATCGATACCATGTATGGTGTTGTCGGCATTCCTATCACGAACCTGGCTCGTATGTGGCAAGATGACGGTCAGCGTTTTTACAGCTTCCGTCACGAACAACACGCAGGTTATGCAGCTTCTATCGCCGGTTACATCGAAGGAAAACCTGGCGTTTGCTTGACCGTTTCCGCCCCTGGCTTCCTGAACGGCGTGACTTCCCTGGCTCATGCAACCACCAACTGCTTCCCAATGATCCTGTTGAGCGGTTCCAGTGAACGTGAAATCGTCGATTTGCAACAGGGCGATTACGAAGAAATGGATCAGATGAATGTTGCACGTCCACACTGCAAAGCTTCTTTCCGTATCAACAGCATCAAAGACATTCCAATCGGTATCGCTCGTGCAGTTCGCACCGCTGTATCCGGACGTCCAGGTGGTGTTTACGTTGACTTGCCAGCAAAACTGTTCGGTCAGACCATTTCTGTAGAAGAAGCTAACAAACTGCTCTTCAAACCAATCGATCCAGCTCCGGCACAGATTCCTGCTGAAGATGCTATCGCTCGCGCTGCTGACCTGATCAAGAACGCCAAACGTCCAGTTATCATGCTGGGTAAAGGCGCTGCATACGCACAATGCGACGACGAAATCCGCGCACTGGTTGAAGAAACCGGCATCCCATTCCTGCCAATGGGTATGGCTAAAGGCCTGCTGCCTGACAACCATCCACAATCCGCTGCTGCAACCCGTGCTTTCGCACTGGCACAGTGTGACGTTTGCGTACTGATCGGCGCTCGTCTGAACTGGCTGATGCAGCACGGTAAAGGCAAAACCTGGGGCGACGAACTGAAGAAATACGTTCAGATCGACATCCAGGCTAACGAAATGGACAGCAACCAGCCTATCGCTGCACCAGTTGTTGGTGACATCAAGTCCGCCGTTTCCCTGCTCCGCAAAGCACTGAAAGGCGCTCCAAAAGCTGACGCTGAATGGACCGGCGCTCTGAAAGCCAAAGTTGACGGCAACAAAGCCAAACTGGCTGGCAAGATGACTGCCGAAACCCCATCCGGAATGATGAACTACTCCAATTCCCTGGGCGTTGTTCGTGACTTCATGCTGGCAAATCCGGATATTTCCCTGGTTAACGAAGGCGCTAATGCACTCGACAACACTCGTATGATTGTTGACATGCTGAAACCACGCAAACGTCTTGACTCCGGTACCTGGGGTGTTATGGGTATTGGTATGGGCTACTGCGTTGCTGCAGCTGCTGTTACCGGCAAACCGGTTATCGCTGTTGAAGGCGATAGCGCATTCGGTTTCTCCGGTATGGAACTGGAAACCATCTGCCGTTACAACCTGCCAGTTACCGTTATCATCATGAACAATGGTGGTATCTATAAAGGTAACGAAGCAGATCCACAACCAGGCGTTATCTCCTGTACCCGTCTGACCCGTGGTCGTTACGACATGATGATGGAAGCATTTGGCGGTAAAGGTTATGTTGCCAATACTCCAGCAGAACTGAAAGCTGCTCTGGAAGAAGCTGTTGCTTCCGGCAAACCATGCCTGATCAACGCGATGATCGATCCAGACGCTGGTGTCGAATCTGGCCGTATCAAGAGCCTGAACGTTGTAAGTAAAGTTGGCAAGAAATAAGGTTCCTAGCTGATTAGAAGGCCATCCTGACGGATGGCCTTTTTTTTGACTGCGTTCCTGGCGTGGTGCTCGCATAGTAGGATAAAACGAAAGGCTCAGTCGAAAGACTGGGCCTTTCGTTTTACAATTGGGCTACCTTTTTTTTGTTTTGTTTGCAATGGTTAATCTATTGTTAAAATTTAAAGTTTCACTTGAACTTTCAAATAATGTTCTTATATTTGCAGTGTCGAAAGAAACAAAGTAGCCTGGATCACACAACATTTAAAAAATAACATTATGAAAGCACTCGAAAAGAGATTCAAAGGTAATATTAACAATAATTTATTTTCAATGACAAGTGCCGCTACTGTTACTGCTTCGTTCAACGATACTTTTAGCGTATCCGATAATGTCGCCGTTATTGTTCCTGAAACGGACACTCAGGTGACCTACAGGGATCTATCCCACATGGTGGGTCACTTCCAGACCATGTTCACAAATCCTAATTCTCCATTGTACGGAGCTGTTTTCAGACAAGATACAGTGGCGATATCCATGCGTAATGGGCTGGAATTTATCGTCGCTTTCCTCGGTGCTACTATGGACGCTAAAATTGGCGCGCCCTTGAATCCCAATTATAAGGAAAAGGAGTTCAATTTTTATTTGAATGACCTGAAATCTAAGGCGATTTGCGTCCCAAAGGGTACCACAAAGTTACAGAGTTCTGAAATTCTAAAATCTGCCTCCACGTTTGGATGTTTTATCGTAGAGCTGGCCTTCGATGCGACCAGGTTTAGGGTAGAGTATGATATATACTCTCCAGAGGACAACTACAAAAGGGTTATTTACCGGTCTTTGAACAACGCCAAATTTGTCAACACAAATCCCGTTAAATTCCCTGGGTTTGCCCGTTCCAGTGACGTTGCCCTGATTTTGCATACCAGTGGTACCACCTCCACTCCAAAAACGGTGCCTTTGTTACATTTGAACATTGTGAGAAGCACGTTGAACATTGCTAACACTTACAAGCTAACGCCCTTGGACAGATCTTATGTCGTGATGCCTCTTTTCCACGTCCATGGGTTAATTGGTGTTTTACTTTCCACTTTTAGAACTCAGGGTTCTGTTGTGGTTCCCGATGGATTCCATCCAAAGTTATTCTGGGACCAATTTGTTAAGTACAACTGTAATTGGTTCAGTTGCGTTCCCACAATAAGCATGATTATGCTGAACATGCCCAAACCAAACCCTTTCCCACACATTAGATTCATCAGATCGTGTTCTTCTGCTTTGGCTCCAGCAACGTTCCATAAGCTGGAGAAGGAATTCAATGCACCTGTCTTGGAGGCCTATGCGATGACCGAAGCATCACATCAAATGACCTCAAACAATCTGCCTCCAGGAAAGAGAAAGCCTGGTACTGTGGGCCAGCCACAAGGAGTCACCGTCGTCATTCTAGATGACAATGACAATGTCTTGCCCCCGGGCAAAGTCGGCGAAGTTTCCATCAGAGGCGAAAACGTCACTTTGGGGTATGCTAATAATCCAAAAGCTAACAAGGAGAACTTCACCAAGAGAGAGAACTATTTCAGAACCGGTGACCAAGGTTATTTCGACCCTGAGGGGTTTTTGGTCCTTACAGGCAGAATCAAAGAGCTTATCAACAGGGGTGGTGAAAAGATTTCACCCATTGAGCTCGACGGCATTATGCTATCGCATCCAAAGATCGATGAAGCCGTTGCATTTGGTGTTCCCGACGATATGTACGGCCAAGTAGTTCAAGCCGCCATTGTTTTGAAGAAGGGAGAAAAAATGACCTACGAAGAACTGGTGAACTTCTTAAAGAAGCACCTAGCCTCTTTCAAAATTCCAACCAAGGTGTACTTTGTTGATAAGCTACCAAAAACCGCTACAGGTAAAATCCAGAGAAGAGTTATCGCAGAAACTTTTGCTAAGAGCAGCAGAAATAAGAGTAAGTTGTAGGGTTCCTAGCTGATTAGAAGGCCATCCTGACGGATGGCCTTTTTTTTGACTGCCCGACGAACGGACTAATCCTGACGTAGGATAAAACGAAAGGCTCAGTCGAAAGACTGGGCCTTTCGTTTTACAATTGGGCTACCTTTTTTTTGTTTTGTTTGCAATGGTTAATCTATTGTTAAAATTTAAAGTTTCACTTGAACTTTCAAATAATGTTCTTATATTTGCAGTGTCGAAAGAAACAAAGTAGCCTGGATCACACAACATTTAAAAAATAACATTATGAAAGCACTCGAAAAGAGATTCAAAGGTAATATTAACAATAATTTATTTTCAATGACCACGACCGCTACCCAACACTTCGCTCAACTTTTACAAAAATATGGCATCGACACAGTCTTTGGAATTGTGGGCATCCCTATTGTCCAACTAGCGGATACGATGGTTGCCAACGGTATCAAATTCATTCCATGCAGGAACGAACAGGCTGCCTCATATGCAGCCTCTGCTTATGGTTATATTAGTGATAAGCCCGGCGTGTTACTTATTGTCGGGGGCCCCGGTTTAATCCACGCACTGGCTGGTATATACAACTCAATGAGTAATAGGTGGCCTCTTCTAGTAATTGCAGGGAGTTCTTCTCAAAGTGATATACATAAAGGCGGGTTTCAAGAGTTGGATCAAGTTAGTCTTTTGTCCCCATTTTTGAAGTTTACCGGAAAGTTAACCCCTGACAATATTGACATGATTACTCAGAAGGCTTTGAATTACTGTATACAAGGCACTGCAGGGGTTTCTTACATAGACGTCCCTGCAGATTTTATTGAGTATGAAAAACCCTTAGAAGGAAACGACCGTACAGGAAATGAATTACCGATGATATTAACTCCAAACATATGTGGCCCTGATCCGTCGAAGATCAAAAAAGTCGTACAGCTTATTTTGCAACATAAAAATAAAAATATTCTGATTGTTATTGGAAAGGGTGCGGTAAAAAACTCACACGAAATCCGCAGACTAGTAAATACGTTCAATCTACCATTTTTACCCACCCCAATGGCTAAGGGAATTGTCCCAGACTCTTCTCCACTGAACGTTTCATCTGCAAGATCTCAAGCATTGAAAATAGCGGATATTGTTCTTGTTCTCGGGGCAAGATTAAACTGGATATTGCATTTTGGTACTTCACCCAAATGGAACTCAGAATCCATATTCATCCAGTTCGATTCCAATCCCGAAACCTTGGGGGATAACAATGTCTCACCTGGTGCTGACCTTTCCATATGGGGAGATATAGGCTTGAGCGTAACCGCTTTAGTTGAGGAATTGACTCGCCAAGATTCCTGCTGGAAGTATAGTGGTGTTAAGCAAGAAATACGAGAGAAAATTCAACTGAATCAAACTCGTCTATTGAGGAAAGAGAAGACTAGAGGGGCACAATTGAATTACAACCAGGTTTATGGAACATTGAGGCCTCTTATCGATGATTACAGGACAATACTTGTGACGGAGGGAGCAAATACTATGGATATTGCGCGTATTTCATTCCCTACAGACGCTCCAAGGCGCCGTTTAGACGCAGGGACCAACGCGACTATGGGGATTGGGCTCGGATATGCTCTTGCATGCAAGGCATCTCATCCGGAACTCGATGTGGTGCTGATTCAGGGTGATTCCGCATTTGGATTCTCTGCCATGGAAATTGAAACGGCAGTAAGGTGTCAGCTGGCATTGGTCATCGTTGTGATGAACAATAGCGGTATTTACCATGGAGAAAAGGATATAGAGGGTGATTTACCTCCAACGGCACTAAGCAAGAACTGCCGTTACGATCTCGTAGGCAAAGGACTGGGTGCCAATGATTTTTTTGTCAACACAATAAGCGAACTGAGCAGATGCTTCCAACAAGCCGTGCAGCTTTCTCGGACTAAAAGAGAAACAAGTGTCATCAACGTTATCATTGAGCCTGGCGAACAAAAGCAAATTGCCTTTGCCTGGCAGAATAAACCGCGTTTATAGGGTTCCTAGCTGATTAGAAGGCCATCCTGACGGATGGCCTTTTTTTTGACTGCTCTGGGTCTGACGGCCATCAAGGGTAGGATAAAACGAAAGGCTCAGTCGAAAGACTGGGCCTTTCGTTTTACAATTGGGCTACCTTTTTTTTGTTTTGTTTGCAATGGTTAATCTATTGTTAAAATTTAAAGTTTCACTTGAACTTTCAAATAATGTTCTTATATTTGCAGTGTCGAAAGAAACAAAGTAGCCTGGATCACACAACATTTAAAAAATAACATTATGAAAGCACTCGAAAAGAGATTCAAAGGTAATATTAACAATAATTTATTTTCAATGGATAGCGATACTCTCTCAGGATTATTGGAAAACGTCGCCAAAAAATTCCCCGATCGCCGAGCTCTCTCCGTTTCTGGAAAATTCAATCTCACTCACGCGCGTCTTCACGATCTAATCGAACGCGCCGCTTCACGCCTTGTCTCCGACGCTGGAATCAAACCCGGCGATGTCGTTGCTCTCACCTTCCCTAACACCGTCGAGTTTGTTATAATGTTTTTAGCGGTGATAAGAGCTAGAGCCACGGCGGCGCCGTTGAACGCAGCGTACACGGCGGAGGAATTTGAGTTTTACCTCTCCGATTCAGATTCAAAGCTATTGTTAACCTCTAAAGAAGGAAACGCACCGGCTCAAGAAGCAGCTTCAAAGCTGAAAATCTCTCACGTCACCGCTACGCTGCTTGACGCTGGCTCGGACCTTGTACTATCCGTTGCGGATTCAGATTCCGTCGTTGACTCAGCGACGGAACTCGTTAATCACCCGGACGACGGTGCTCTCTTCCTCCACACTTCTGGCACTACGAGCCGTCCAAAGGGTGTACCGCTTACGCAGCTCAATCTAGCTTCATCCGTCAAGAACATTAAAGCTGTGTACAAGCTTACTGAGTCTGATTCTACGGTGATTGTTCTCCCTCTGTTCCATGTTCATGGATTGTTAGCTGGGTTGCTTAGCTCGCTTGGAGCTGGTGCTGCTGTAACTCTTCCAGCTGCTGGTAGATTCTCAGCAACAACATTTTGGCCAGATATGAAGAAGTATAACGCTACATGGTATACTGCTGTGCCGACCATTCATCAGATCATATTGGACCGCCACGCGAGCCACCCTGAAACGGAATATCCTAAACTCCGGTTCATCAGGAGTTGCAGTGCTTCTTTGGCTCCGGTGATATTGTCCAGGCTTGAGGAAGCGTTTGGAGCACCGGTGCTCGAGGCCTATGCAATGACAGAGGCAACACATTTGATGAGCTCAAACCCCTTACCAGAGGAAGGTCCACACAAGCCTGGGTCTGTTGGGAAACCGGTAGGTCAAGAAATGGCGATCCTTAATGAGAAAGGCGAGATCCAAGAGCCAAATAACAAAGGAGAGGTTTGTATAAGAGGTCCAAATGTGACCAAGGGTTACAAGAATAACCCAGAAGCCAACAAGGCAGGTTTCGAGTTTGGGTGGTTCCACACTGGTGATATCGGTTACTTTGATACCGATGGGTATTTGCATCTGGTGGGTCGGATCAAAGAGCTTATTAACCGTGGAGGTGAGAAGATATCTCCAATTGAAGTGGATGCAGTACTCTTAACGCATCCTGACGTTTCTCAGGGTGTTGCATTCGGTGTTCCTGATGAGAAATATGGGGAAGAGATTAACTGTGCGGTGATTCCAAGAGAAGGAACTACTGTAACCGAAGAGGACATTAAAGCGTTTTGTAAGAAGAATTTGGCAGCTTTCAAGGTGCCAAAGAGAGTGTTCATCACTGATAACCTCCCCAAAACTGCCTCTGGTAAGATTCAGCGCCGTATCGTCGCACAACATTTCCTTGAGAAGCCCTGAGGTTCCTAGCTGATTAGAAGGCCATCCTGACGGATGGCCTTTTTTTTGACTGGGTGTTCCGATACACTCAATTTGTGTAGGATAAAACGAAAGGCTCAGTCGAAAGACTGGGCCTTTCGTTTTACAATTGGGCTACCTTTTTTTTGTTTTGTTTGCAATGGTTAATCTATTGTTAAAATTTAAAGTTTCACTTGAACTTTCAAATAATGTTCTTATATTTGCAGTGTCGAAAGAAACAAAGTAGCCTGGATCACACAACATTTAAAAAATAACATTATGAAAGCACTCGAAAAGAGATTCAAAGGTAATATTAACAATAATTTATTTTCAATGACAAATAATGAAAGCAAAGGGCCGTTTGAAGGCTTATTAGTTATCGATATGACACATGTCCTTAATGGACCTTTCGGAACTCAACTTCTTTGTAATATGGGCGCAAGGGTAATTAAAGTTGAGCCGCCGGGTCATGGTGATGATACCCGCACATTTGGTCCCTATGTGGATGGACAGTCACTCTATTACAGTTTTATTAATCATGGCAAAGAGAGTGTGGTTCTTGATTTAAAGAATGATCACGATAAAAGTATATTTATAAATATGCTCAAACAAGCTGATGTATTAGCTGAGAATTTTCGCCCAGGTACAATGGAAAAACTGGGGTTTTCATGGGAAACGCTTCAAGAAATCAACCCGCGCCTCATATATGCTTCATCGTCAGGTTTCGGACATACCGGTCCGCTAAAAGATGCTCCTGCCTACGATACCATCATTCAGGCAATGAGCGGGATAATGATGGAAACAGGATATCCTGATGCTCCGCCAGTGCGCGTTGGTACATCTCTTGCGGATCTATGCGGCGGTGTCTATTTATTCAGCGGAATAGTGAGTGCACTTTATGGCCGCGAAAAGAGCCAGAGAGGGGCGCATGTCGATATAGCGATGTTTGATGCCACGCTGAGTTTTCTGGAGCATGGTCTGATGGCATATATCGCAACTGGGAAGTCACCACAACGTCTGGGAAATCGCCATCCCTACATGGCACCTTTTGATGTTTTCAATACTCAGGATAAGCCGATTACGATTTGTTGTGGTAATGACAAGCTTTTTTCTGCGTTATGCCAGGCACTGGAGCTTACGGAACTGGTTAATGATCCCCGATTTAGCAGCAATATTTTACGCGTACAAAACCAGGCTATTCTTAAACAATATATTGAGCGGACGTTAAAAACGCAGGCAGCTGAAGTTTGGTTAGCCAGAATACATGAAGTTGGTGTACCCGTCGCGCCGTTATTAAGTGTGGCTGAGGCCATTAAATTGCCACAAACTCAGGCGAGAAATATGTTGATTGAAGCCGGGGGAATAATGATGCCGGGTAATCCGATAAAAATCAGCGGCTGCGCGGACCCGCATGTTATGCCGGGAGCGGCAACGCTCGACCAGCATGGGGAACAAATTCGCCAGGAGTTCTCATCATAAGGTTCCTAGCTGATTAGAAGGCCATCCTGACGGATGGCCTTTTTTTTGACTTAGTTTCAATTTTACATTCCAGGAAGTAGGATAAAACGAAAGGCTCAGTCGAAAGACTGGGCCTTTCGTTTTACAATTGGGCTACCTTTTTTTTGTTTTGTTTGCAATGGTTAATCTATTGTTAAAATTTAAAGTTTCACTTGAACTTTCAAATAATGTTCTTATATTTGCAGTGTCGAAAGAAACAAAGTAGCCTGGATCACACAACATTTAAAAAATAACATTATGAAAGCACTCGAAAAGAGATTCAAAGGTAATATTAACAATAATTTATTTTAATGAATAATCCACAAACAGGACAATCAACAGGCCTCTTGGGCAATCGTTGGTTCTACTTGGTATTAGCAGTTTTGCTGATGTGTATGATCTCGGGTGTCCAATATTCCTGGACACTGTACGCTAACCCGGTTAAAGACAACCTTGGCGTTTCTTTGGCTGCGGTTCAGACGGCTTTCACACTCTCTCAGGTCATTCAAGCTGGTTCTCAGCCTGGTGGTGGTTACTTCGTTGATAAATTCGGTCCAAGAATTCCATTGATGTTCGGTGGTGCGATGGTTCTCGCTGGCTGGACCTTCATGGGTATGGTTGACAGTGTTCCTGCTCTGTATGCTCTTTATACTCTGGCCGGTGCAGGTGTTGGTATCGTTTACGGTATCGCGATGAACACGGCTAACAGATGGTTCCCGGACAAACGCGGTCTGGCTTCCGGTTTCACCGCTGCCGGTTACGGTCTGGGTGTTCTGCCGTTCCTGCCACTGATCAGCTCCGTTCTGAAAGTTGAAGGTGTTGGCGCAGCATTCATGTACACCGGTTTGATCATGGGTATCCTGATTATCCTGATCGCTTTCGTTATCCGTTTCCCTGGCCAGCAAGGCGCCAAAAAACAAATCGTTGTTACCGACAAGGATTTCAATTCTGGCGAAATGCTGAGAACACCACAATTCTGGGTTCTGTGGACCGCATTCTTTTCCGTTAACTTTGGTGGTTTGCTGCTGGTTGCCAACAGCGTCCCTTACGGTCGCAGCCTCGGTCTTGCCGCAGGTGTGCTGACGATCGGTGTTTCGATCCAGAACCTGTTCAATGGTGGTTGCCGTCCTTTCTGGGGTTTCGTTTCCGATAAAATCGGCCGTTACAAAACCATGTCCGTCGTTTTCGGTATCAATGCTGTTGTTCTCGCACTTTTCCCGACGATTGCTGCCTTGGGCGATGTAGCCTTTATCGCCATGTTGGCAATCGCATTCTTCACATGGGGTGGTAGCTACGCTCTGTTCCCATCGACCAACAGCGATATTTTCGGTACGGCATACTCTGCCAGAAACTATGGTTTCTTCTGGGCTGCAAAAGCAACTGCCTCGATCTTCGGTGGTGGTCTGGGTGCTGCAATTGCAACCAACTTCGGATGGAATACCGCTTTCCTGATTACTGCGATTACTTCTTTCATCGCATTTGCTCTGGCTACCTTCGTTATTCCAAGAATGGGCCGTCCAGTCAAGAAAATGGTCAAATTGTCTCCAGAAGAAAAAGCTGTACATTAAGGTTCCTAGCTGATTAGAAGGCCATCCTGACGGATGGCCTTTTTTTTGACTGCTATGACTAAACGGCGCGCCTGATAGGTGGGCTGCCCTTCCTGGTTGGCTTGGTTTCATCAGCCATCCGCTTGCCCTCATCTGTTACGCCGGCGGTAGCCGGCCAGCCTCGCAGAGCAGGATTCCCGTTGAGCACCGCCAGGTGCGAATAAGGGACAGTGAAGAAGGAACACCCGCTCGCGGGTGGGCCTACTTCACCTATCCTGCCCGGCTGACGCCGTTGGATACACCAAGGAAAGTCTACACGAACCCTTTGGCAAAATCCTGTATATCGTGCGAAAAAGGATGGATATACCGAAAAAATCGCTATAATGACCCCGAAGCAGGGTTATGCAGCGGAAAAGTTATATACATTCATGTCCATTTATGTAAAAAATCCTGCTGACCTTGTTTATGTCTTGTCAGTCACCATTTGCAAAACCATATTTGACCCTCAAAGAGGCTGAATTTGATAAGCAACTTGCTACATACTCATAATAAGGAGCTAAATAGAACACGAATGGGAAATACTCAAATGCCAAACTAAAGAAGATATTGGCCAAAATAAACGCTATACCGAGAGAGAAACTTGATTTTTCAACTTCCTAAAACAGTGTTGTTCAAACATTTCTACTTATTTGTACTTACCAGTTGAACCTACGTTTCCCTAATAAAATGTCTATGGTAAAAAGTTAAAAAATCCTCCTACTTTTGTTAGATATATTTTTTTGTGTAATTTTGTAATCGTTATGCGGCAGTAATAATATACATATTAATACGAGTTAGGAATCCTGTAGTTCTCATATGCTACGAGGAGGTATTAAAAGGTGCGTTTCGACAATGCATCTATTGTAGTATATTATTGCTTAATCCAAATGAATATTATAAATTTAGGAATTCTTGCTCACATTGATGCAGGAAAAACTTCCGTAACCGAGAATCTGCTGTTTGCCAGTGGAGCAACGGAAAAGTGCGGCTGTGTGGATAATGGTGACACCATAACGGACTCTATGGATATAGAGAAACGTAGAGGAATTACTGTTCGGGCTTCTACGACATCTATTATCTGGAATGGTGTGAAATGCAATATCATTGACACTCCGGGACACATGGATTTTATTGCGGAAGTGGAGCGGACATTCAAAATGCTTGATGGAGCAGTCCTCATCTTATCCGCAAAGGAAGGCATACAAGCGCAGACAAAGTTGCTGTTCAATACTTTACAGAAGCTGCAAATCCCGACAATTATATTTATCAATAAGATTGACCGAGCCGGTGTGAATTTGGAGCGTTTGTATCTGGATATAAAAGCAAATCTGTCTCAAGATGTCCTGTTTATGCAAAATGTTGTCGATGGATCGGTTTATCCGGTTTGCTCCCAAACATATATAAAGGAAGAATACAAAGAATTTGTATGCAACCATGACGACAATATATTAGAACGATATTTGGCGGATAGCGAAATTTCACCGGCTGATTATTGGAATACGATAATCGCTCTTGTGGCAAAAGCCAAAGTCTATCCGGTGCTACATGGATCAGCAATGTTCAATATCGGTATCAATGAGTTGTTGGACGCCATCACTTCTTTTATACTTCCTCCGGCATCGGTTTCAAACAGACTTTCATCTTATCTTTATAAGATAGAGCATGACCCCAAAGGACATAAAAGAAGTTTTCTAAAAATAATTGACGGAAGTCTGAGACTTCGAGATGTTGTAAGAATCAACGATTCGGAAAAATTCATCAAGATTAAAAATCTAAAAACTATCAATCAGGGCAGAGAGATAAATGTTGATGAAGTGGGCGCCAATGATATCGCGATTGTAGAGGATATGGATGATTTTCGAATCGGAAATTATTTAGGTGCTGAACCTTGTTTGATTCAAGGATTATCGCATCAGCATCCCGCTCTCAAATCCTCCGTCCGGCCAGACAGGCCCGAAGAGAGAAGCAAGGTGATATCCGCTCTGAATACATTGTGGATTGAAGATCCGTCTTTGTCCTTTTCCATAAACTCATATAGTGATGAATTGGAAATCTCGTTATATGGTTTAACCCAAAAGGAAATCATACAGACATTGCTGGAAGAACGATTTTCCGTAAAGGTCCATTTTGATGAGATCAAGACTATATACAAAGAACGACCTGTAAAAAAGGTCAATAAGATTATTCAGATCGAAGTGCCGCCCAACCCTTATTGGGCCACAATAGGGCTGACTCTTGAACCCTTACCGTTAGGGACAGGGTTGCAAATCGAAAGTGACATCTCCTATGGTTATCTGAACCATTCTTTTCAAAATGCCGTTTTTGAAGGGATTCGTATGTCTTGCCAATCCGGGTTACATGGATGGGAAGTGACTGATCTGAAAGTAACTTTTACTCAAGCCGAGTATTATAGCCCGGTAAGTACACCAGCTGATTTCAGACAGCTGACCCCTTATGTCTTTAGGCTGGCCTTGCAACAGTCAGGTGTGGACATTCTCGAACCGATGCTCTATTTTGAGTTGCAGATACCCCAAGCGGCAAGTTCCAAAGCTATTACAGATTTGCAAAAAATGATGTCTGAGATTGAAGATATCAGTTGCAATAATGAGTGGTGTCATATTAAAGGGAAAGTTCCATTAAATACAAGTAAAGACTATGCATCAGAAGTAAGTTCATACACTAAGGGCTTAGGCATTTTTATGGTTAAGCCATGCGGGTATCAAATAACAAAAGGCGGTTATTCTGATAATATCCGCATGAACGAAAAAGATAAACTTTTATTCATGTTCCAAAAATCAATGTCATCAAAATAACCACGAGTCATTGGTAACTATCTATGAAACTGTTTGATACTTTTATAGTTGATTAAACTTGTTCATGGCATTTGCCTTAATATCATCCGCTATGTCAATGTAGGGTTTCATAGCTTTGTAGTCGCTGTGTCCCGTCCATTTCATGACCACCTGTGCCGGGATTCCGAGAGCCAGCGCATTGCAGATGAATGTCCTTTTTCCTGCATGGGTACTGAGCAAAGCGTATTTGGGTGTGACTTCATCAATACGTTCATTTCCCTTGTAGTAGGTTTCCCGTACAGGCTCGTTGATTTCTGCCAGTTCGCCCAGCTCTTTCAGGTAATCGTTCATCTTCTGGTTGCTGATGACGGGCAGAGCCATGTAATTCTCGAAATGGATGTCCTTGTATTTGTCCAGTATGGCTTTGCTGTATTTGTTCAGTTCAATCGTCAGGCTGTCGGCAGTCTTGACTGTGGTTATTTCGATGTGGTCGGACTTCACATCGCTTCTTTTCAGATTGCGAACATCCGAATACCGCAAACTCGTAAAGCAGCAGAACAGGAAAACATCACGCACACGTTCCAGGTATTGCTTATCCTTGGGTATCTGGTAGTCTTTCAGCTTGTTCAGTTCATCCCAAGTCAGGAAGATTACTTTTTTCGAGGTGGTTTTCAGTTTCGGTTTGAACGTATCGTATGCAATGTTCTGATGATGTCCTTTCTTGAAGCTCCAGCGCAGGAACCATTTGAGGAATCCCATTTGCTTGCCGATGGTGCTGTTTCTCATATCCTTGGTGTCACGCAGGAAGTTGACGTATTCGTTCAATCCAAACTCGTTGAAATAGTTGAACGTTGCATCCTCCTTGAACTCTTTGAGGTGGTTCCTCACTGCTGCAAATTTTTCATAGGTGGATGCCGTCCAGTTATTCTGGTTACCGCACTCTTTTACAAACTCATCGAACACCTCCCAAAAGCTGACAGGGGCTTCTTCCGGCTGTTCTTCACTGGTATCTTTCATTCTCATGTTGAAAGCTTCCTTCAACTGTTGGGTCGTTGGCATGACCTCCTGCACCTCAAATTCCTTGAAAATATTCTGGATTTCGGCATAGTATTTCAGCAAGTCCGTATTGATTTCGGCTGCACTTTGCTTTAGCTTGTTGGTACATCCGTTCTTTACCCGCTGCTTATCTGCATCCCATTTGGCTACGTCAATCCGGTAGCCCGTTGTAAACTCGATACGTTGGCTGGCAAAGATGACACGCATACGGATGGGTACGTTCTCTACGATTGGCACACCGTTCTTTTTCCGGCTCTCCAATGCAAAAATGATGTTGCGCTTGATATTCATAATTGGGTGCGTTTGAAATTCTACACCCAAATATACACCCAATTATTGAGATAGCAAAAGACATTTAGAAACATTTACTTTTACTCTATATTGTAATTTACACTTGATTATCAGTCGTTTGCAGTCTTATGATATTCTGTGAAAGTATAAGTTCGAGAGCCTGTCTCTCCGCAAAAAACGCTGAAAATCAGCAGATTGCAAAACAAACACCCTGTTTTACACCCAAGAATGTAAAGTCGGGTGTTTTTGTTTTATTTAAGATAATACAACCACTACATAATAAAAGAGTAGCGATATTAAAAGAATCCGATGAGAAAAGACTAATATTTATCTATCCATTCAGTTTGATTTCTCAGGACTTTACATCGTCCTGAAAGTATTTGTTGCCAGTGTTACAACCAATTAACCAATTCTGATTAGAAAAACTCATCGAGCATCAAATGAAACTGCAATTTATTCATATCAGGATTATCAATACCATATTTTTGAAAAAGCCGTTTCTGTAATGAAGGAGAAAACTCACCGAGGCAGTTCCATAGGATGGCAAGATCCTGGTATCGGTCTGCGATTCCGACTCGTCCAACATCAATACAACCTATTAATTTCCCCTCGTCAAAAATAAGGTTATCAAGTGAGAAATCACCATGAGTGACGACTGAATCCGGTGAGAATGGCAAAAGCTTATGCATTTCTTTCCAGACTTGTTCAACAGGCCAGCCATTACGCTCGTCATCAAAATCACTCGCATCAACCAAACCGTTATTCATTCGTGATTGCGCCTGAGCGAGGCGAAATACGCGATCGCTGTTAAAAGGACAATTACAAACAGGAATCGAATGCAACCGGCGCAGGAACACTGCCAGCGCATCAACAATATTTTCACCTGAATCAGGATATTCTTCTAATACCTGGAATGCTGTTTTCCCGGGGATCGCAGTGGTGAGTAACCATGCATCATCAGGAGTACGGATAAAATGCTTGATGGTCGGAAGAGGCATAAATTCCGTCAGCCAGTTTAGTCTGACCATCTCATCTGTAACATCATTGGCAACGCTACCTTTGCCATGTTTCAGAAACAACTCTGGCGCATCGGGCTTCCCATACAATCGATAGATTGTCGCACCTGATTGCCCGACATTATCGCGAGCCCATTTATACCCATATAAATCAGCATCCATGTTGGAATTTAATCGCGGCCTGGAGCAAGACGTTTCCCGTTGAATATGGCTCATAACACCCCTTGTATTACTGTTTATGTAAGCAGACAGTTTTATTGTTCATGATGATATATTTTTATCTTGTGCAATGTAACATCAGAGATTTTGAGACACAACGTGGCTTTGTTGAATAAATCGAACTTTTGCTGAGTTGAAGGATCAGCCGCGCAGTTCAACCTGTTGATAGTACGTACTAAGCTCTCATGTTTCACGTACTAAGCTCTCATGTTTAACGTACTAAGCTCTCATGTTTAACGAACTAAACCCTCATGGCTAACGTACTAAGCTCTCATGGCTAACGTACTAAGCTCTCATGTTTCACGTACTAAGCTCTCATGTTTGAACAATAAAATTAATATAAATCAGCAACTTAAATAGCCTCTAAGGTTTTAAGTTTTATAAGAAAAAAAAGAATATATAAGGCTTTTAAAGCTTTTAAGGTTTAACGGTTGTGGACAACAAGCCAGGGATGTAACGCACTGAGAAGCCCTTAGAGCCTCTCAAAGCAATTTTGAGTGACACAGGAACACTTAACGGCTGACATGGGGCGGCCGCTCAAGTAG
SEQ ID NO:78(RBS和N-端前导肽)：
GATCACACAACATTTAAAAAATAACATTATGAAAGCACTCGAAAAGAGATTCAAAGGTAATATTAACAATAATTTATTTTCA
SEQ ID NO:79(RBS和N-端NanoLuc萤光素酶报告基因)：
TCCATCAATTTAAAATTTAAAATAATGGTTTTTACTCTGGAAGATTTTGTTGGCGATTGGCGTCAGACCGCGGGTTATAATTTGGATCAAGTCCTGGAACAGGGTGGCGTAAGCTCTCTGTTCCAGAACCTGGGTGTGAGCGTGACGCCGATTCAGCGCATCGTTCTGTCCGGCGAGAACGGTCTGAAAATTGATATTCATGTGATCATCCCGTACGAAGGCCTGAGCGGTGACCAAATGGGTCAAATCGAGAAAATCTTTAAAGTCGTCTACCCAGTTGACGATCACCACTTCAAGGTTATCTTGCATTACGGTACGCTGGTGATTGATGGTGTGACCCCGAATATGATTGACTATTTCGGCCGTCCGTATGAAGGCATTGCCGTTTTTGACGGTAAAAAGATCACCGTCACCGGTACCCTGTGGAATGGCAATAAGATTATTGACGAGCGTCTGATTAACCCGGACGGCAGCCTGCTGTTCCGCGTGACCATCAACGGTGTCACGGGTTGGCGTCTGTGCGAGCGCATCCTGGCA
SEQ ID NO:80(P_BfP2E5)：
GATAAAACGAAAGGCTCAGTCGAAAGACTGGGCCTTTCGTTTTACAATTGGGCTACCTTTTTTTTGTTTTGTTTGCAATGGTTAATCTATTGTTAAAATTTAAAGTTTCACTTGAACTTTCAAATAATGTTCTTATATTTCCAGTGTCGAAAGAAACAAAGTAG
SEQ ID NO:81(用于基因组整合进普通拟杆菌基因组中的编码草酸盐降解途径的质粒)：
GGAGAGCTGTATGTGGGGTGGAAGAAGCTATCGTAAAAATGCTGGAGCAGCTTGATCCTCATTCTACCTATTCTGATCCGGAGGAAGTGAAGAGAATGAACGAGCCGTTGCAGGGAAACTTCGAAGGTATCGGTATACAGTTTAATATGGCGGAGGACACTTTGCTGGTGATACAGCCTGTATCGGGCGGACCGTCCGAAAAAGTCGGTATTCTGGCCGGTGATCGTATCGTCATGGTGGAAGATACGCTGATAGCCGGTGTAAAGATGAGTACCGAGGATATTATGCGTCGCCTCCGTGGTCCTAAAGATTCAAAAGTGAATCTGAAAATACTTCGCCGAGGGGTGAAGGAACTGCTCCCCTTTACGGTGAAAAGGGATAAAATACCTGTTTACAGCTTGGATGCTTCCTATATGATTAAAGATAAGATCGGTTATATTCGTATCAACCGTTTTGCCGCCACTACCCATGAGGAGTTCAAGAAAGCGCTGACCGGGTTGCAGAAGAAAGGAATGAAAGACTTGATTCTTGATTTGCAGGGCAATGGCGGGGGGTATTTGAATGCTGCTATCGATATTGCCAATGAATTTTTGGGTAAAAAGGAACTGATTGTCTATACGGAGGGCCGTCGTAATCCTCGTAGCGAGTTTTTTGCCAAAGGTACGGGCGAGTTTCAGAACGGTCGTCTGATGGTATTGGTTGATGAATTTTCGGCTTCTGCCAGTGAGATTGTGACCGGAGCTGTTCAGGATTGGGACAGAGGCGTTGTGGTAGGCCGTCGCAGTTTTGGTAAAGGCTTGGTGCAGCGTCCCATTGATTTGCCGGACGGTTCGATGATCCGTCTGACGGTGGCCCGCTATTATACGCCTGCCGGACGTTGTATCCAGAAACCTTACGAGAGCATTGAAAAGTATAATGAAGACCTGATAGACCGTTATAATAAAGGAGAAATGATGAGTGAAGACAGCATCCACTTTCCGGATTCACTGAAGTTCAAGACTCATAGGTTGGCCCGTACGGTATATGGTGGCGGAGGGATCATGCCCGATTATTTTGTGCCGATTGATACGACTCTCTATACTAAATACCATCGTCAGTTGCGTGATAAAGGAGCATTGATGAAAGCTCATTTCCATTTTATTGATGCCCATCGTAAAGAATGGCTGGGCAAATACAAGACTTTCAATGAGTTCTACAAACGTTTTGAAGTGACTCCGGATATGTTGACACAACTGGTTGCTACCGGCAAGGAGATGGGAGTGGAATATAATGAAGAAGAGTATCAAAAGGCCCTGCCTCTGCTGAGGTTGCAGATGAAAGCATTGATAGCCCGTGATTTATGGGATATGAATGAATATTATCACGTCATTAATGACGCGAATGAAAGTATCAGGAAAGCGCTGGAACTGTTGGAGCAACCGGACTTTGAGGGGCTGCTGTTGAAAAAGCGATAGTCAAGACTTTATATTAACTGCAGGGGCGCATAGTTGCACTCCTGCAGTTGTCTGAAAAACTTGTAGGATAAAACGAAAGGCTCAGTCGAAAGACTGGGCCTTTCGTTTTACAATTGGGCTACCTTTTTTTTGTTTTGTTTGCAATGGTTAATCTATTGTTAAAATTTAAAGTTTCACTTGAACTTTCAAATAATGTTCTTATATTTGCAGTGTCGAAAGAAACAAAGTAGCCTGGCATCTTAATCAATTTTAAACTCACCAAATCAAAATATGAAAAAAAAAAATAATAAAATGTCAACTCCACTTCAAGGAATTAAAGTTCTCGATTTCACCGGTGTGCAATCTGGCCCATCTTGTACTCAAATGCTGGCCTGGTTTGGCGCTGACGTTATTAAAATTGAACGTCCCGGCGTTGGTGACGTAACGCGTCACCAGCTGCGAGATATTCCTGATATCGATGCGCTTTACTTCACCATGCTTAACAGTAACAAACGTTCTATTGAGTTAAATACCAAAACAGCGGAAGGCAAAGAGGTAATGGAAAAGCTGATCCGCGAAGCTGATATCTTAGTCGAGAACTTTCATCCAGGGGCCATTGATCACATGGGCTTCACCTGGGAGCATATTCAAGAAATCAATCCACGTCTGATTTTTGGTTCGATCAAAGGGTTTGATGAGTGTTCGCCTTATGTGAATGTAAAAGCCTATGAAAACGTTGCTCAGGCAGCGGGTGGCGCGGCATCCACTACGGGTTTTTGGGATGGTCCGCCGCTGGTAAGCGCTGCAGCGTTGGGTGACAGCAACACCGGAATGCATTTGCTGATCGGTTTACTTGCTGCTTTGCTGCATCGCGAAAAAACGGGGCGTGGGCAACGAGTCACCATGTCAATGCAGGATGCCGTATTGAACCTTTGCCGCGTGAAATTACGTGACCAGCAGCGTCTAGATAAATTGGGTTATCTGGAAGAATACCCGCAGTATCCGAATGGTACATTTGGTGATGCAGTTCCCCGCGGTGGTAATGCAGGTGGTGGCGGTCAGCCTGGCTGGATCCTGAAATGTAAAGGCTGGGAAACCGATCCTAACGCCTATATTTATTTCACTATTCAGGAGCAAAACTGGGAAAACACCTGTAAAGCCATCGGCAAACCAGAATGGATTACCGATCCGGCATACAGTACAGCCCATGCACGACAGCCACATATTTTCGATATTTTTGCTGAAATCGAAAAATACACTGTCACTATTGATAAACATGAAGCGGTGGCCTATTTGACTCAGTTTGATATTCCTTGTGCACCGGTTTTAAGTATGAAAGAAATTTCACTTGATCCCTCTTTGCGCCAAAGTGGCAGTGTTGTTGAAGTGGAACAACCGTTGCGTGGAAAATATCTGACCGTTGGTTGTCCAATGAAATTCTCTGCCTTTACGCCGGATATTAAAGCTGCGCCGCTATTAGGTGAACATACCGCTGCTGTATTGCAGGAGCTGGGTTATAGCGACGATGAAATTGCTGCAATGAAGCAAAACCACGCCATCTGAGGTTCCTAACCTGCCTGGCATCAACTACCTAAATTAATTATCACCCCAAATTAATAACATAAAAATATAATAAAATGAGTAACGACGACAATGTAGAGTTGACTGATGGCTTTCATGTTTTGATCGATGCCCTGAAAATGAATGACATCGATACCATGTATGGTGTTGTCGGCATTCCTATCACGAACCTGGCTCGTATGTGGCAAGATGACGGTCAGCGTTTTTACAGCTTCCGTCACGAACAACACGCAGGTTATGCAGCTTCTATCGCCGGTTACATCGAAGGAAAACCTGGCGTTTGCTTGACCGTTTCCGCCCCTGGCTTCCTGAACGGCGTGACTTCCCTGGCTCATGCAACCACCAACTGCTTCCCAATGATCCTGTTGAGCGGTTCCAGTGAACGTGAAATCGTCGATTTGCAACAGGGCGATTACGAAGAAATGGATCAGATGAATGTTGCACGTCCACACTGCAAAGCTTCTTTCCGTATCAACAGCATCAAAGACATTCCAATCGGTATCGCTCGTGCAGTTCGCACCGCTGTATCCGGACGTCCAGGTGGTGTTTACGTTGACTTGCCAGCAAAACTGTTCGGTCAGACCATTTCTGTAGAAGAAGCTAACAAACTGCTCTTCAAACCAATCGATCCAGCTCCGGCACAGATTCCTGCTGAAGATGCTATCGCTCGCGCTGCTGACCTGATCAAGAACGCCAAACGTCCAGTTATCATGCTGGGTAAAGGCGCTGCATACGCACAATGCGACGACGAAATCCGCGCACTGGTTGAAGAAACCGGCATCCCATTCCTGCCAATGGGTATGGCTAAAGGCCTGCTGCCTGACAACCATCCACAATCCGCTGCTGCAACCCGTGCTTTCGCACTGGCACAGTGTGACGTTTGCGTACTGATCGGCGCTCGTCTGAACTGGCTGATGCAGCACGGTAAAGGCAAAACCTGGGGCGACGAACTGAAGAAATACGTTCAGATCGACATCCAGGCTAACGAAATGGACAGCAACCAGCCTATCGCTGCACCAGTTGTTGGTGACATCAAGTCCGCCGTTTCCCTGCTCCGCAAAGCACTGAAAGGCGCTCCAAAAGCTGACGCTGAATGGACCGGCGCTCTGAAAGCCAAAGTTGACGGCAACAAAGCCAAACTGGCTGGCAAGATGACTGCCGAAACCCCATCCGGAATGATGAACTACTCCAATTCCCTGGGCGTTGTTCGTGACTTCATGCTGGCAAATCCGGATATTTCCCTGGTTAACGAAGGCGCTAATGCACTCGACAACACTCGTATGATTGTTGACATGCTGAAACCACGCAAACGTCTTGACTCCGGTACCTGGGGTGTTATGGGTATTGGTATGGGCTACTGCGTTGCTGCAGCTGCTGTTACCGGCAAACCGGTTATCGCTGTTGAAGGCGATAGCGCATTCGGTTTCTCCGGTATGGAACTGGAAACCATCTGCCGTTACAACCTGCCAGTTACCGTTATCATCATGAACAATGGTGGTATCTATAAAGGTAACGAAGCAGATCCACAACCAGGCGTTATCTCCTGTACCCGTCTGACCCGTGGTCGTTACGACATGATGATGGAAGCATTTGGCGGTAAAGGTTATGTTGCCAATACTCCAGCAGAACTGAAAGCTGCTCTGGAAGAAGCTGTTGCTTCCGGCAAACCATGCCTGATCAACGCGATGATCGATCCAGACGCTGGTGTCGAATCTGGCCGTATCAAGAGCCTGAACGTTGTAAGTAAAGTTGGCAAGAAATAAGGTTCCTAAACGACCTGGCATCACCTAAAAATTATAAATAAATTAATAAACTTTTATTAAAAAAAAATTACAAAATGAATAATCCACAAACAGGACAATCAACAGGCCTCTTGGGCAATCGTTGGTTCTACTTGGTATTAGCAGTTTTGCTGATGTGTATGATCTCGGGTGTCCAATATTCCTGGACACTGTACGCTAACCCGGTTAAAGACAACCTTGGCGTTTCTTTGGCTGCGGTTCAGACGGCTTTCACACTCTCTCAGGTCATTCAAGCTGGTTCTCAGCCTGGTGGTGGTTACTTCGTTGATAAATTCGGTCCAAGAATTCCATTGATGTTCGGTGGTGCGATGGTTCTCGCTGGCTGGACCTTCATGGGTATGGTTGACAGTGTTCCTGCTCTGTATGCTCTTTATACTCTGGCCGGTGCAGGTGTTGGTATCGTTTACGGTATCGCGATGAACACGGCTAACAGATGGTTCCCGGACAAACGCGGTCTGGCTTCCGGTTTCACCGCTGCCGGTTACGGTCTGGGTGTTCTGCCGTTCCTGCCACTGATCAGCTCCGTTCTGAAAGTTGAAGGTGTTGGCGCAGCATTCATGTACACCGGTTTGATCATGGGTATCCTGATTATCCTGATCGCTTTCGTTATCCGTTTCCCTGGCCAGCAAGGCGCCAAAAAACAAATCGTTGTTACCGACAAGGATTTCAATTCTGGCGAAATGCTGAGAACACCACAATTCTGGGTTCTGTGGACCGCATTCTTTTCCGTTAACTTTGGTGGTTTGCTGCTGGTTGCCAACAGCGTCCCTTACGGTCGCAGCCTCGGTCTTGCCGCAGGTGTGCTGACGATCGGTGTTTCGATCCAGAACCTGTTCAATGGTGGTTGCCGTCCTTTCTGGGGTTTCGTTTCCGATAAAATCGGCCGTTACAAAACCATGTCCGTCGTTTTCGGTATCAATGCTGTTGTTCTCGCACTTTTCCCGACGATTGCTGCCTTGGGCGATGTAGCCTTTATCGCCATGTTGGCAATCGCATTCTTCACATGGGGTGGTAGCTACGCTCTGTTCCCATCGACCAACAGCGATATTTTCGGTACGGCATACTCTGCCAGAAACTATGGTTTCTTCTGGGCTGCAAAAGCAACTGCCTCGATCTTCGGTGGTGGTCTGGGTGCTGCAATTGCAACCAACTTCGGATGGAATACCGCTTTCCTGATTACTGCGATTACTTCTTTCATCGCATTTGCTCTGGCTACCTTCGTTATTCCAAGAATGGGCCGTCCAGTCAAGAAAATGGTCAAATTGTCTCCAGAAGAAAAAGCTGTACATTAAGGTTCCTAATTCCCCTGGCATCATCTACCTTTATTAATCAACAATCCAAATTATTAATAAAAAAAATTTAAAAAATGACAAATAATGAAAGCAAAGGGCCGTTTGAAGGCTTATTAGTTATCGATATGACACATGTCCTTAATGGACCTTTCGGAACTCAACTTCTTTGTAATATGGGCGCAAGGGTAATTAAAGTTGAGCCGCCGGGTCATGGTGATGATACCCGCACATTTGGTCCCTATGTGGATGGACAGTCACTCTATTACAGTTTTATTAATCATGGCAAAGAGAGTGTGGTTCTTGATTTAAAGAATGATCACGATAAAAGTATATTTATAAATATGCTCAAACAAGCTGATGTATTAGCTGAGAATTTTCGCCCAGGTACAATGGAAAAACTGGGGTTTTCATGGGAAACGCTTCAAGAAATCAACCCGCGCCTCATATATGCTTCATCGTCAGGTTTCGGACATACCGGTCCGCTAAAAGATGCTCCTGCCTACGATACCATCATTCAGGCAATGAGCGGGATAATGATGGAAACAGGATATCCTGATGCTCCGCCAGTGCGCGTTGGTACATCTCTTGCGGATCTATGCGGCGGTGTCTATTTATTCAGCGGAATAGTGAGTGCACTTTATGGCCGCGAAAAGAGCCAGAGAGGGGCGCATGTCGATATAGCGATGTTTGATGCCACGCTGAGTTTTCTGGAGCATGGTCTGATGGCATATATCGCAACTGGGAAGTCACCACAACGTCTGGGAAATCGCCATCCCTACATGGCACCTTTTGATGTTTTCAATACTCAGGATAAGCCGATTACGATTTGTTGTGGTAATGACAAGCTTTTTTCTGCGTTATGCCAGGCACTGGAGCTTACGGAACTGGTTAATGATCCCCGATTTAGCAGCAATATTTTACGCGTACAAAACCAGGCTATTCTTAAACAATATATTGAGCGGACGTTAAAAACGCAGGCAGCTGAAGTTTGGTTAGCCAGAATACATGAAGTTGGTGTACCCGTCGCGCCGTTATTAAGTGTGGCTGAGGCCATTAAATTGCCACAAACTCAGGCGAGAAATATGTTGATTGAAGCCGGGGGAATAATGATGCCGGGTAATCCGATAAAAATCAGCGGCTGCGCGGACCCGCATGTTATGCCGGGAGCGGCAACGCTCGACCAGCATGGGGAACAAATTCGCCAGGAGTTCTCATCATAAGGTTCCTAAAAACCCTGGCATCATCTACCTATATTAATCATCAACCCAATTTTCATATGTATAAAATTAACAAAATGACAAGTGCCGCTACTGTTACTGCTTCGTTCAACGATACTTTTAGCGTATCCGATAATGTCGCCGTTATTGTTCCTGAAACGGACACTCAGGTGACCTACAGGGATCTATCCCACATGGTGGGTCACTTCCAGACCATGTTCACAAATCCTAATTCTCCATTGTACGGAGCTGTTTTCAGACAAGATACAGTGGCGATATCCATGCGTAATGGGCTGGAATTTATCGTCGCTTTCCTCGGTGCTACTATGGACGCTAAAATTGGCGCGCCCTTGAATCCCAATTATAAGGAAAAGGAGTTCAATTTTTATTTGAATGACCTGAAATCTAAGGCGATTTGCGTCCCAAAGGGTACCACAAAGTTACAGAGTTCTGAAATTCTAAAATCTGCCTCCACGTTTGGATGTTTTATCGTAGAGCTGGCCTTCGATGCGACCAGGTTTAGGGTAGAGTATGATATATACTCTCCAGAGGACAACTACAAAAGGGTTATTTACCGGTCTTTGAACAACGCCAAATTTGTCAACACAAATCCCGTTAAATTCCCTGGGTTTGCCCGTTCCAGTGACGTTGCCCTGATTTTGCATACCAGTGGTACCACCTCCACTCCAAAAACGGTGCCTTTGTTACATTTGAACATTGTGAGAAGCACGTTGAACATTGCTAACACTTACAAGCTAACGCCCTTGGACAGATCTTATGTCGTGATGCCTCTTTTCCACGTCCATGGGTTAATTGGTGTTTTACTTTCCACTTTTAGAACTCAGGGTTCTGTTGTGGTTCCCGATGGATTCCATCCAAAGTTATTCTGGGACCAATTTGTTAAGTACAACTGTAATTGGTTCAGTTGCGTTCCCACAATAAGCATGATTATGCTGAACATGCCCAAACCAAACCCTTTCCCACACATTAGATTCATCAGATCGTGTTCTTCTGCTTTGGCTCCAGCAACGTTCCATAAGCTGGAGAAGGAATTCAATGCACCTGTCTTGGAGGCCTATGCGATGACCGAAGCATCACATCAAATGACCTCAAACAATCTGCCTCCAGGAAAGAGAAAGCCTGGTACTGTGGGCCAGCCACAAGGAGTCACCGTCGTCATTCTAGATGACAATGACAATGTCTTGCCCCCGGGCAAAGTCGGCGAAGTTTCCATCAGAGGCGAAAACGTCACTTTGGGGTATGCTAATAATCCAAAAGCTAACAAGGAGAACTTCACCAAGAGAGAGAACTATTTCAGAACCGGTGACCAAGGTTATTTCGACCCTGAGGGGTTTTTGGTCCTTACAGGCAGAATCAAAGAGCTTATCAACAGGGGTGGTGAAAAGATTTCACCCATTGAGCTCGACGGCATTATGCTATCGCATCCAAAGATCGATGAAGCCGTTGCATTTGGTGTTCCCGACGATATGTACGGCCAAGTAGTTCAAGCCGCCATTGTTTTGAAGAAGGGAGAAAAAATGACCTACGAAGAACTGGTGAACTTCTTAAAGAAGCACCTAGCCTCTTTCAAAATTCCAACCAAGGTGTACTTTGTTGATAAGCTACCAAAAACCGCTACAGGTAAAATCCAGAGAAGAGTTATCGCAGAAACTTTTGCTAAGAGCAGCAGAAATAAGAGTAAGTTGTAGGGTTCCTAGCTGATTAGAAGGCCATCCTGACGGATGGCCTTTTTTTTGACTCTGTTGTGTCTTCTTTTAGGTGTGGAATAATTTTCCAACCATCACTTTATCTGTCTCCCGAACAGCACCTTATTCAGCCATCGGTTCACATACACGTTCACGAAAGCACATCCTACTCCGATAGCGGCTCCTGCCACTACATCACTGGGATAGTGCACCCCTTGGTTCATCCGGGCAAACCCTACTCCGCAAGCCCATACGGCTGAAGGGGCGATAACATACCACTTAGGATAAGTGATGGAGAGTGAGGTGGCTAAAGAAAAAGCTGCCGCAGTGTGTCCTGAAGGGAATGACGGACTATCGGCATCCGGTGCTCCGTATGCATGAATCTTATCCGGATACTTTACAAAAGGCCGGTCACGACCTATTATATGTTTGGCGGCGTATGTAATCCCCACTGCTTCAATCACACTGGTTCCTATGTAAATGGCATCTTTCAGCATCGGCTGGTCTTTCTTTATCAATGCATAGAGTCCCATGGCTGTAGGTATTCCTACGGGTAGAATCACTCCGCTAGCGGACAATCCGCGGCTCAGTCCGTGTACATCCCATCGGTTGATGGTTTTCAACGTGTTGATGTCTATATTCTGTGCGCTGAGGCTGAATGTAAAACAGGCTGTCAGTATAAGAAAAATAATCCTTTGGCGTTTCATTTAGTTTGGCATTTGTTCAGTGCAAAGATAGGAAAATAATGTAATTCGCATTTACCCTTCCCCGAATTAAAGAATAATTTCCATAATTCCTTGTATTATCGCTAGAATTCCTTTACTTTTGCATCGTGAATACACAGCTTTTAACAGAATTTTTAGTTCTAACAATTAAATAATTTAAATTCAATCATTTATGTGGTTAAGTAATTCATCTATTGGAAGGAAAGTGGTGATGAGTGTCACAGGTATCGCCCTTGTCCTGTTTCTGACGTTTCACATGGCGATGAACCTTGTTGCGCTCTTCTCGGAAGATGCTTACAACATGATTTGTGAATTCTTGGGAGCTAACTGGTACGCGTTGGTTGCAACTGCCGGGCTGGCTGCTCTGTTTGTAATTCACATCATCTACGCTTTCTGGTTGACCATGCAGAACCGTGCAGCTCGCGGTAGCGAACGTTATGCAGTGAACGTTAAACCTAAAACTGTGGAATGGGCATCTCAGAACATGCTTGTCCTGGGTATTGTCGTTATTGCCGGATTGGCTCTTCATTTTGCCAATTTCTGGTACAAAATGCAGTTTGCCGAAATTATCGGTGTTCATGATTTGGGCGCATTCGGTCCTACAGACGGTGCCGCTTACATTCGTGAAACATTCGGATGCCCTGTATTTACAGTGCTTTACCTCATTTGGTTAGCAGCTTTGTGGTTCCACCTGACTCACGGATTCTGGAGTGCTTTGCAGACTTTGGGCTGGAGCAATAAAATCTGGTTCGAGCGTTGGAAGACTATTGGTAACATCTACTCTACTTTGGTAGTTCTTGGTTTTGCAGCAGTAGTCGTTATCTACTTCGCTATGACTGGCGCGCCTGATAGGTGGGCTGCCCTTCCTGGTTGGCTTGGTTTCATCAGCCATCCGCTTGCCCTCATCTGTTACGCCGGCGGTAGCCGGCCAGCCTCGCAGAGCAGGATTCCCGTTGAGCACCGCCAGGTGCGAATAAGGGACAGTGAAGAAGGAACACCCGCTCGCGGGTGGGCCTACTTCACCTATCCTGCCCGGCTGACGCCGTTGGATACACCAAGGAAAGTCTACACGAACCCTTTGGCAAAATCCTGTATATCGTGCGAAAAAGGATGGATATACCGAAAAAATCGCTATAATGACCCCGAAGCAGGGTTATGCAGCGGAAAAGCGGGATTAAAAGTCGGGGATTGGTGAACAAAAAGGTGTTTCTCTCTTTAAGAGAAATATCGTTTTGCTAAACAGTTGATATTGAGGTATCATTTTATCGTAAAAGACATTTTTGCTCAACAATTGCTTGACGGAAATCAACAAATTTTAGCATTTTGTAAAAAAGTCGCTATATAATTTGGTGAATTGGAGTTATTTTCATATTTTTGCATCCCGAAGAGTTTCTCTTAAAGAGAGAAACATCTTTTGCATACCTTTTCCGACCGAATTTTTATGTCGTAAAGAGGGGCTTTGCAGGGGGTGGACTCAGAAAGATGAGAATAGATGACTATTGTAGTTGAAACACATAGAAAGTTGCTGATATACAGACCGATACGCATATCGGGATGAACCATGAGTACGTTCTTTTCTCAAAAAACATAAATATTCGAAAAGAGATGCAATAAATTAAGGAGAGGTTATAATGAACAAAGTAAATATAAAAGATAGTCAAAATTTTATTACTTCAAAATATCACATAGAAAAAATAATGAATTGCATAAGTTTAGATGAAAAAGATAACATCTTTGAAATAGGTGCAGGGAAAGGTCATTTTACTGCTGGATTGGTAAAGAGATGTAATTTTGTAACGGCGATAGAAATTGATTCTAAATTATGTGAGGTAACTCGTAATAAGCTCTTAAATTATCCTAACTATCAAATAGTAAATGATGATATACTGAAATTTACATTTCCTAGCCACAATCCATATAAAATATTTGGCAGCATACCTTACAACATAAGCACAAATATAATTCGAAAAATTGTTTTTGAAAGTTCAGCCACAATAAGTTATTTAATAGTGGAATATGGTTTTGCTAAAATGTTATTAGATACAAACAGATCACTAGCATTGCTGTTAATGGCAGAGGTAGATATTTCTATATTAGCAAAAATTCCTAGGTATTATTTCCATCCAAAACCTAAAGTGGATAGCACATTAATTGTATTAAAAAGAAAGCCAGCAAAAATGGCATTTAAAGAGAGAAAAAAATATGAAACTTTTGTAATGAAATGGGTTAACAAAGAGTACGAAAAACTGTTTACAAAAAATCAATTTAATAAAGCTTTAAAACATGCGAGAATATATGATATAAACAATATTAGTTTCGAACAATTTGTATCGCTATTTAATAGTTATAAAATATTTAACGGCTAAAAACAATAGGCCACATGCAACTGTAAATGTTTACGCGGGTACCGACACCGCGGTGGAGGGGAATTGTGTTACAACCAATTAACCAATTCTGATTAGAAAAACTCATCGAGCATCAAATGAAACTGCAATTTATTCATATCAGGATTATCAATACCATATTTTTGAAAAAGCCGTTTCTGTAATGAAGGAGAAAACTCACCGAGGCAGTTCCATAGGATGGCAAGATCCTGGTATCGGTCTGCGATTCCGACTCGTCCAACATCAATACAACCTATTAATTTCCCCTCGTCAAAAATAAGGTTATCAAGTGAGAAATCACCATGAGTGACGACTGAATCCGGTGAGAATGGCAAAAGCTTATGCATTTCTTTCCAGACTTGTTCAACAGGCCAGCCATTACGCTCGTCATCAAAATCACTCGCATCAACCAAACCGTTATTCATTCGTGATTGCGCCTGAGCGAGGCGAAATACGCGATCGCTGTTAAAAGGACAATTACAAACAGGAATCGAATGCAACCGGCGCAGGAACACTGCCAGCGCATCAACAATATTTTCACCTGAATCAGGATATTCTTCTAATACCTGGAATGCTGTTTTCCCGGGGATCGCAGTGGTGAGTAACCATGCATCATCAGGAGTACGGATAAAATGCTTGATGGTCGGAAGAGGCATAAATTCCGTCAGCCAGTTTAGTCTGACCATCTCATCTGTAACATCATTGGCAACGCTACCTTTGCCATGTTTCAGAAACAACTCTGGCGCATCGGGCTTCCCATACAATCGATAGATTGTCGCACCTGATTGCCCGACATTATCGCGAGCCCATTTATACCCATATAAATCAGCATCCATGTTGGAATTTAATCGCGGCCTGGAGCAAGACGTTTCCCGTTGAATATGGCTCATAACACCCCTTGTATTACTGTTTATGTAAGCAGACAGTTTTATTGTTCATGATGATATATTTTTATCTTGTGCAATGTAACATCAGAGATTTTGAGACACAACGTGGCTTTGTTGAATAAATCGAACTTTTGCTGAGTTGAAGGATCAGAGCCTACGTTCCGAATACGGTCAAAAAAAAGGCCATCCGTCAGGATGGCCTTCGCATTAATATGCCGCTTCGAATTCTTTTAGGAAGCGTGTATCGTTTTCAGAGAACATACGGAGGTCTTTCACCTGATATTTCAGGTTTGTGATACGCTCGATACCCATACCGAGTCCATAACCGCTGTATATTTTGCTGTCTATACCATTTGATTCAAGTACGTTCGGGTCTACCATACCGCAACCGAGGATTTCTACCCAGCCGGTGTGTTTACAGAACGGACATCCTTTACCGCCGCAGATATTACAGCTGATATCCATTTCCGCACTTGGTTCAGCAAACGGGAAGTAAGACGGACGCAGACGGATCTTTGTATCAGCACCGAACATTTCTTTGGCAAAGAGCAGCAATACCTGCTTCAAGTCGGTGAATGATACGTTTTTATCTACATACAGCGCTTCTACCTGATGGAAGAAACAGTGTGCGCGATAGCTGATAGCTTCGTTACGATATACACGTCCCGGACAGATGATGCGGATAGGAGGCTGTGAAGTTTCCATCACACGAGTCTGTACAGAAGAAGTATGTGTACGCAATACTACGTCCGGGTGAGCTTCGATAAAGAAAGTGTCCTGCATATCGCGTGCCGGATGATCTTCGGCAAAGTTCAGTGCCGAGAACACGTGCCAGTCATCTTCAATTTCCGGACCTTCGGCAATGCTGAATCCCAGACGGGCAAAGATATCAATGATTTCGTTCTTTACAATGGTGAGCGGGTGGCGTGTACCGAGTTCTACAGGATAAGCCGAACGCGTCAAATCCAGTCCGTCACAATCGTTGTCCTGACTTTCAAACATTTCTTTCAGCGCGTTGATTTTGTCCTGCGCTTTTGTTTTCAGTTCATTCAGTCTCATGCCGACTTCTTTTTTCTGTTCGGCAGCTACATTACGGAAATCTGCCATTAAGTCGTTAATGGCTCCCTTCTTACTTAGGTATTTGATGCGGAGAGCTTCGAGTTCTTCGGCATTGGAGGCGTGTAAGGCTTCCACCTCTTTCAGAAGTTGTTCAATCTTAGCTATCATTTTTTAATATTTTTAGCGGCCCCGTTAAACAAAATTATTTGTAGAGGCTGTTTCGTCCTCACGGACTCATCAGACCGGAAAGCACATCCGGTGACAGCTCAGGCTACTTTGTTTCTTTCGACACTGCAAATATAAGAACATTATTTGAAAGTTCAAGTGAAACTTTAAATTTTAACAATAGATTAACCATTGCAAACAAAACAAAAAAAAGGTAGCCCAATTGTAAAACGAAAGGCCCAGTCTTTCGACTGAGCCTTTCGTTTTATCCTACAGTCGCTCGGCGATCGAAGGCTTCGGAAAAAAAAGGCCATCCGTCAGGATGGCCTTCGCATTAATATGCCGCTTCGAATTCTTTTAGGAAGCGTGTATCGTTTTCAGAGAACATACGGAGGTCTTTCACCTGATATTTCAGGTTTGTGATACGCTCGATACCCATACCGAGTCCATAACCGCTGTATATTTTGCTGTCTATACCATTTGATTCAAGTACGTTCGGGTCTACCATACCGCAACCGAGGATTTCTACCCAGCCGGTGTGTTTACAGAACGGACATCCTTTACCGCCGCAGATATTACAGCTGATATCCATTTCCGCACTTGGTTCAGCAAACGGGAAGTAAGACGGACGCAGACGGATCTTTGTATCAGCACCGAACATTTCTTTGGCAAAGAGCAGCAATACCTGCTTCAAGTCGGTGAATGATACGTTTTTATCTACATACAGCGCTTCTACCTGATGGAAGAAACAGTGTGCGCGATAGCTGATAGCTTCGTTACGATATACACGTCCCGGACAGATGATGCGGATAGGAGGCTGTGAAGTTTCCATCACACGAGTCTGTACAGAAGAAGTATGTGTACGCAATACTACGTCCGGGTGAGCTTCGATAAAGAAAGTGTCCTGCATATCGCGTGCCGGATGATCTTCGGCAAAGTTCAGTGCCGAGAACACGTGCCAGTCATCTTCAATTTCCGGACCTTCGGCAATGCTGAATCCCAGACGGGCAAAGATATCAATGATTTCGTTCTTTACAATGGTGAGCGGGTGGCGTGTACCGAGTTCTACAGGATAAGCCGAACGCGTCAAATCCAGTCCGTCACAATCGTTGTCCTGACTTTCAAACATTTCTTTCAGCGCGTTGATTTTGTCCTGCGCTTTTGTTTTCAGTTCATTCAGTCTCATGCCGACTTCTTTTTTCTGTTCGGCAGCTACATTACGGAAATCTGCCATTAAGTCGTTAATGGCTCCCTTCTTACTTAGGTATTTGATGCGGAGAGCTTCGAGTTCTTCGGCATTGGAGGCGTGTAAGGCTTCCACCTCTTTCAGAAGTTGTTCAATCTTAGCTATCATTTTTTAATATTTTTAGCGGCCCCGTTAAACAAAATTATTTGTAGAGGCTGTTTCGTCCTCACGGACTCATCAGACCGGAAAGCACATCCGGTGACAGCTCAGGCTACTTTGTTTCTTTCGACACTGCAAATATAAGAACATTATTTGAAAGTTCAAGTGAAACTTTAAATTTTAACAATAGATTAACCATTGCAAACAAAACAAAAAAAAGGTAGCCCAATTGTAAAACGAAAGGCCCAGTCTTTCGACTGAGCCTTTCGTTTTATCCTAGGATCAGCTGTACGTACTCGCAGTTCAACCTGTTGATAGTACGTACTAAGCTCTCATGTTTCACGTACTAAGCTCTCATGTTTAACGTACTAAGCTCTCATGTTTAACGAACTAAACCCTCATGGCTAACGTACTAAGCTCTCATGGCTAACGTACTAAGCTCTCATGTTTCACGTACTAAGCTCTCATGTTTGAACAATAAAATTAATATAAATCAGCAACTTAAATAGCCTCTAAGGTTTTAAGTTTTATAAGAAAAAAAAGAATATATAAGGCTTTTAAAGCTTTTAAGGTTTAACGGTTGTGGACAACAAGCCAGGGATGTAACGCACTGAGAAGCCCTTAGAGCCTCTCAAAGCAATTTTGAGTGACACAGGAACACTTAACGGCTGACATGGGGCGGCCGCGTAG
SEQ ID NO:82(用于基因组整合进普通拟杆菌基因组中的编码草酸盐降解途径的质粒)：
GGAGAGCTGTATGTGGGGTGGAAGAAGCTATCGTAAAAATGCTGGAGCAGCTTGATCCTCATTCTACCTATTCTGATCCGGAGGAAGTGAAGAGAATGAACGAGCCGTTGCAGGGAAACTTCGAAGGTATCGGTATACAGTTTAATATGGCGGAGGACACTTTGCTGGTGATACAGCCTGTATCGGGCGGACCGTCCGAAAAAGTCGGTATTCTGGCCGGTGATCGTATCGTCATGGTGGAAGATACGCTGATAGCCGGTGTAAAGATGAGTACCGAGGATATTATGCGTCGCCTCCGTGGTCCTAAAGATTCAAAAGTGAATCTGAAAATACTTCGCCGAGGGGTGAAGGAACTGCTCCCCTTTACGGTGAAAAGGGATAAAATACCTGTTTACAGCTTGGATGCTTCCTATATGATTAAAGATAAGATCGGTTATATTCGTATCAACCGTTTTGCCGCCACTACCCATGAGGAGTTCAAGAAAGCGCTGACCGGGTTGCAGAAGAAAGGAATGAAAGACTTGATTCTTGATTTGCAGGGCAATGGCGGGGGGTATTTGAATGCTGCTATCGATATTGCCAATGAATTTTTGGGTAAAAAGGAACTGATTGTCTATACGGAGGGCCGTCGTAATCCTCGTAGCGAGTTTTTTGCCAAAGGTACGGGCGAGTTTCAGAACGGTCGTCTGATGGTATTGGTTGATGAATTTTCGGCTTCTGCCAGTGAGATTGTGACCGGAGCTGTTCAGGATTGGGACAGAGGCGTTGTGGTAGGCCGTCGCAGTTTTGGTAAAGGCTTGGTGCAGCGTCCCATTGATTTGCCGGACGGTTCGATGATCCGTCTGACGGTGGCCCGCTATTATACGCCTGCCGGACGTTGTATCCAGAAACCTTACGAGAGCATTGAAAAGTATAATGAAGACCTGATAGACCGTTATAATAAAGGAGAAATGATGAGTGAAGACAGCATCCACTTTCCGGATTCACTGAAGTTCAAGACTCATAGGTTGGCCCGTACGGTATATGGTGGCGGAGGGATCATGCCCGATTATTTTGTGCCGATTGATACGACTCTCTATACTAAATACCATCGTCAGTTGCGTGATAAAGGAGCATTGATGAAAGCTCATTTCCATTTTATTGATGCCCATCGTAAAGAATGGCTGGGCAAATACAAGACTTTCAATGAGTTCTACAAACGTTTTGAAGTGACTCCGGATATGTTGACACAACTGGTTGCTACCGGCAAGGAGATGGGAGTGGAATATAATGAAGAAGAGTATCAAAAGGCCCTGCCTCTGCTGAGGTTGCAGATGAAAGCATTGATAGCCCGTGATTTATGGGATATGAATGAATATTATCACGTCATTAATGACGCGAATGAAAGTATCAGGAAAGCGCTGGAACTGTTGGAGCAACCGGACTTTGAGGGGCTGCTGTTGAAAAAGCGATAGTCAAGACTTTATATTAACTGCAGGGGCGCATAGTTGCACTCCTGCAGTTGTCTGAAAAACTTGTAGGATAAAACGAAAGGCTCAGTCGAAAGACTGGGCCTTTCGTTTTACAATTGGGCTACCTTTTTTTTGTTTTGTTTGCAATGGTTAATCTATTGTTAAAATTTAAAGTTTCACTTGAACTTTCAAATAATGTTCTTATATTTGCAGTGTCGAAAGAAACAAAGTAGCCTGGCATCTTAATCAATTTTAAACTCACCAAATCAAAATATGAAAAAAAAAAATAATAAAATGTCAACTCCACTTCAAGGAATTAAAGTTCTCGATTTCACCGGTGTGCAATCTGGCCCATCTTGTACTCAAATGCTGGCCTGGTTTGGCGCTGACGTTATTAAAATTGAACGTCCCGGCGTTGGTGACGTAACGCGTCACCAGCTGCGAGATATTCCTGATATCGATGCGCTTTACTTCACCATGCTTAACAGTAACAAACGTTCTATTGAGTTAAATACCAAAACAGCGGAAGGCAAAGAGGTAATGGAAAAGCTGATCCGCGAAGCTGATATCTTAGTCGAGAACTTTCATCCAGGGGCCATTGATCACATGGGCTTCACCTGGGAGCATATTCAAGAAATCAATCCACGTCTGATTTTTGGTTCGATCAAAGGGTTTGATGAGTGTTCGCCTTATGTGAATGTAAAAGCCTATGAAAACGTTGCTCAGGCAGCGGGTGGCGCGGCATCCACTACGGGTTTTTGGGATGGTCCGCCGCTGGTAAGCGCTGCAGCGTTGGGTGACAGCAACACCGGAATGCATTTGCTGATCGGTTTACTTGCTGCTTTGCTGCATCGCGAAAAAACGGGGCGTGGGCAACGAGTCACCATGTCAATGCAGGATGCCGTATTGAACCTTTGCCGCGTGAAATTACGTGACCAGCAGCGTCTAGATAAATTGGGTTATCTGGAAGAATACCCGCAGTATCCGAATGGTACATTTGGTGATGCAGTTCCCCGCGGTGGTAATGCAGGTGGTGGCGGTCAGCCTGGCTGGATCCTGAAATGTAAAGGCTGGGAAACCGATCCTAACGCCTATATTTATTTCACTATTCAGGAGCAAAACTGGGAAAACACCTGTAAAGCCATCGGCAAACCAGAATGGATTACCGATCCGGCATACAGTACAGCCCATGCACGACAGCCACATATTTTCGATATTTTTGCTGAAATCGAAAAATACACTGTCACTATTGATAAACATGAAGCGGTGGCCTATTTGACTCAGTTTGATATTCCTTGTGCACCGGTTTTAAGTATGAAAGAAATTTCACTTGATCCCTCTTTGCGCCAAAGTGGCAGTGTTGTTGAAGTGGAACAACCGTTGCGTGGAAAATATCTGACCGTTGGTTGTCCAATGAAATTCTCTGCCTTTACGCCGGATATTAAAGCTGCGCCGCTATTAGGTGAACATACCGCTGCTGTATTGCAGGAGCTGGGTTATAGCGACGATGAAATTGCTGCAATGAAGCAAAACCACGCCATCTGAGGTTCCTAACCTGCCTGGCATCAACTACCTAAATTAATTATCACCCCAAATTAATAACATAAAAATATAATAAAATGAGTAACGACGACAATGTAGAGTTGACTGATGGCTTTCATGTTTTGATCGATGCCCTGAAAATGAATGACATCGATACCATGTATGGTGTTGTCGGCATTCCTATCACGAACCTGGCTCGTATGTGGCAAGATGACGGTCAGCGTTTTTACAGCTTCCGTCACGAACAACACGCAGGTTATGCAGCTTCTATCGCCGGTTACATCGAAGGAAAACCTGGCGTTTGCTTGACCGTTTCCGCCCCTGGCTTCCTGAACGGCGTGACTTCCCTGGCTCATGCAACCACCAACTGCTTCCCAATGATCCTGTTGAGCGGTTCCAGTGAACGTGAAATCGTCGATTTGCAACAGGGCGATTACGAAGAAATGGATCAGATGAATGTTGCACGTCCACACTGCAAAGCTTCTTTCCGTATCAACAGCATCAAAGACATTCCAATCGGTATCGCTCGTGCAGTTCGCACCGCTGTATCCGGACGTCCAGGTGGTGTTTACGTTGACTTGCCAGCAAAACTGTTCGGTCAGACCATTTCTGTAGAAGAAGCTAACAAACTGCTCTTCAAACCAATCGATCCAGCTCCGGCACAGATTCCTGCTGAAGATGCTATCGCTCGCGCTGCTGACCTGATCAAGAACGCCAAACGTCCAGTTATCATGCTGGGTAAAGGCGCTGCATACGCACAATGCGACGACGAAATCCGCGCACTGGTTGAAGAAACCGGCATCCCATTCCTGCCAATGGGTATGGCTAAAGGCCTGCTGCCTGACAACCATCCACAATCCGCTGCTGCAACCCGTGCTTTCGCACTGGCACAGTGTGACGTTTGCGTACTGATCGGCGCTCGTCTGAACTGGCTGATGCAGCACGGTAAAGGCAAAACCTGGGGCGACGAACTGAAGAAATACGTTCAGATCGACATCCAGGCTAACGAAATGGACAGCAACCAGCCTATCGCTGCACCAGTTGTTGGTGACATCAAGTCCGCCGTTTCCCTGCTCCGCAAAGCACTGAAAGGCGCTCCAAAAGCTGACGCTGAATGGACCGGCGCTCTGAAAGCCAAAGTTGACGGCAACAAAGCCAAACTGGCTGGCAAGATGACTGCCGAAACCCCATCCGGAATGATGAACTACTCCAATTCCCTGGGCGTTGTTCGTGACTTCATGCTGGCAAATCCGGATATTTCCCTGGTTAACGAAGGCGCTAATGCACTCGACAACACTCGTATGATTGTTGACATGCTGAAACCACGCAAACGTCTTGACTCCGGTACCTGGGGTGTTATGGGTATTGGTATGGGCTACTGCGTTGCTGCAGCTGCTGTTACCGGCAAACCGGTTATCGCTGTTGAAGGCGATAGCGCATTCGGTTTCTCCGGTATGGAACTGGAAACCATCTGCCGTTACAACCTGCCAGTTACCGTTATCATCATGAACAATGGTGGTATCTATAAAGGTAACGAAGCAGATCCACAACCAGGCGTTATCTCCTGTACCCGTCTGACCCGTGGTCGTTACGACATGATGATGGAAGCATTTGGCGGTAAAGGTTATGTTGCCAATACTCCAGCAGAACTGAAAGCTGCTCTGGAAGAAGCTGTTGCTTCCGGCAAACCATGCCTGATCAACGCGATGATCGATCCAGACGCTGGTGTCGAATCTGGCCGTATCAAGAGCCTGAACGTTGTAAGTAAAGTTGGCAAGAAATAAGGTTCCTAAACGACCTGGCATCACCTAAAAATTATAAATAAATTAATAAACTTTTATTAAAAAAAAATTACAAAATGAATAATCCACAAACAGGACAATCAACAGGCCTCTTGGGCAATCGTTGGTTCTACTTGGTATTAGCAGTTTTGCTGATGTGTATGATCTCGGGTGTCCAATATTCCTGGACACTGTACGCTAACCCGGTTAAAGACAACCTTGGCGTTTCTTTGGCTGCGGTTCAGACGGCTTTCACACTCTCTCAGGTCATTCAAGCTGGTTCTCAGCCTGGTGGTGGTTACTTCGTTGATAAATTCGGTCCAAGAATTCCATTGATGTTCGGTGGTGCGATGGTTCTCGCTGGCTGGACCTTCATGGGTATGGTTGACAGTGTTCCTGCTCTGTATGCTCTTTATACTCTGGCCGGTGCAGGTGTTGGTATCGTTTACGGTATCGCGATGAACACGGCTAACAGATGGTTCCCGGACAAACGCGGTCTGGCTTCCGGTTTCACCGCTGCCGGTTACGGTCTGGGTGTTCTGCCGTTCCTGCCACTGATCAGCTCCGTTCTGAAAGTTGAAGGTGTTGGCGCAGCATTCATGTACACCGGTTTGATCATGGGTATCCTGATTATCCTGATCGCTTTCGTTATCCGTTTCCCTGGCCAGCAAGGCGCCAAAAAACAAATCGTTGTTACCGACAAGGATTTCAATTCTGGCGAAATGCTGAGAACACCACAATTCTGGGTTCTGTGGACCGCATTCTTTTCCGTTAACTTTGGTGGTTTGCTGCTGGTTGCCAACAGCGTCCCTTACGGTCGCAGCCTCGGTCTTGCCGCAGGTGTGCTGACGATCGGTGTTTCGATCCAGAACCTGTTCAATGGTGGTTGCCGTCCTTTCTGGGGTTTCGTTTCCGATAAAATCGGCCGTTACAAAACCATGTCCGTCGTTTTCGGTATCAATGCTGTTGTTCTCGCACTTTTCCCGACGATTGCTGCCTTGGGCGATGTAGCCTTTATCGCCATGTTGGCAATCGCATTCTTCACATGGGGTGGTAGCTACGCTCTGTTCCCATCGACCAACAGCGATATTTTCGGTACGGCATACTCTGCCAGAAACTATGGTTTCTTCTGGGCTGCAAAAGCAACTGCCTCGATCTTCGGTGGTGGTCTGGGTGCTGCAATTGCAACCAACTTCGGATGGAATACCGCTTTCCTGATTACTGCGATTACTTCTTTCATCGCATTTGCTCTGGCTACCTTCGTTATTCCAAGAATGGGCCGTCCAGTCAAGAAAATGGTCAAATTGTCTCCAGAAGAAAAAGCTGTACATTAAGGTTCCTAATTCCCCTGGCATCATCTACCTTTATTAATCAACAATCCAAATTATTAATAAAAAAAATTTAAAAAATGACAAATAATGAAAGCAAAGGGCCGTTTGAAGGCTTATTAGTTATCGATATGACACATGTCCTTAATGGACCTTTCGGAACTCAACTTCTTTGTAATATGGGCGCAAGGGTAATTAAAGTTGAGCCGCCGGGTCATGGTGATGATACCCGCACATTTGGTCCCTATGTGGATGGACAGTCACTCTATTACAGTTTTATTAATCATGGCAAAGAGAGTGTGGTTCTTGATTTAAAGAATGATCACGATAAAAGTATATTTATAAATATGCTCAAACAAGCTGATGTATTAGCTGAGAATTTTCGCCCAGGTACAATGGAAAAACTGGGGTTTTCATGGGAAACGCTTCAAGAAATCAACCCGCGCCTCATATATGCTTCATCGTCAGGTTTCGGACATACCGGTCCGCTAAAAGATGCTCCTGCCTACGATACCATCATTCAGGCAATGAGCGGGATAATGATGGAAACAGGATATCCTGATGCTCCGCCAGTGCGCGTTGGTACATCTCTTGCGGATCTATGCGGCGGTGTCTATTTATTCAGCGGAATAGTGAGTGCACTTTATGGCCGCGAAAAGAGCCAGAGAGGGGCGCATGTCGATATAGCGATGTTTGATGCCACGCTGAGTTTTCTGGAGCATGGTCTGATGGCATATATCGCAACTGGGAAGTCACCACAACGTCTGGGAAATCGCCATCCCTACATGGCACCTTTTGATGTTTTCAATACTCAGGATAAGCCGATTACGATTTGTTGTGGTAATGACAAGCTTTTTTCTGCGTTATGCCAGGCACTGGAGCTTACGGAACTGGTTAATGATCCCCGATTTAGCAGCAATATTTTACGCGTACAAAACCAGGCTATTCTTAAACAATATATTGAGCGGACGTTAAAAACGCAGGCAGCTGAAGTTTGGTTAGCCAGAATACATGAAGTTGGTGTACCCGTCGCGCCGTTATTAAGTGTGGCTGAGGCCATTAAATTGCCACAAACTCAGGCGAGAAATATGTTGATTGAAGCCGGGGGAATAATGATGCCGGGTAATCCGATAAAAATCAGCGGCTGCGCGGACCCGCATGTTATGCCGGGAGCGGCAACGCTCGACCAGCATGGGGAACAAATTCGCCAGGAGTTCTCATCATAAGGTTCCTAAAAACCCTGGCATCATCTACCTATATTAATCATCAACCCAATTTTCATATGTATAAAATTAACAAAATGACAAGTGCCGCTACTGTTACTGCTTCGTTCAACGATACTTTTAGCGTATCCGATAATGTCGCCGTTATTGTTCCTGAAACGGACACTCAGGTGACCTACAGGGATCTATCCCACATGGTGGGTCACTTCCAGACCATGTTCACAAATCCTAATTCTCCATTGTACGGAGCTGTTTTCAGACAAGATACAGTGGCGATATCCATGCGTAATGGGCTGGAATTTATCGTCGCTTTCCTCGGTGCTACTATGGACGCTAAAATTGGCGCGCCCTTGAATCCCAATTATAAGGAAAAGGAGTTCAATTTTTATTTGAATGACCTGAAATCTAAGGCGATTTGCGTCCCAAAGGGTACCACAAAGTTACAGAGTTCTGAAATTCTAAAATCTGCCTCCACGTTTGGATGTTTTATCGTAGAGCTGGCCTTCGATGCGACCAGGTTTAGGGTAGAGTATGATATATACTCTCCAGAGGACAACTACAAAAGGGTTATTTACCGGTCTTTGAACAACGCCAAATTTGTCAACACAAATCCCGTTAAATTCCCTGGGTTTGCCCGTTCCAGTGACGTTGCCCTGATTTTGCATACCAGTGGTACCACCTCCACTCCAAAAACGGTGCCTTTGTTACATTTGAACATTGTGAGAAGCACGTTGAACATTGCTAACACTTACAAGCTAACGCCCTTGGACAGATCTTATGTCGTGATGCCTCTTTTCCACGTCCATGGGTTAATTGGTGTTTTACTTTCCACTTTTAGAACTCAGGGTTCTGTTGTGGTTCCCGATGGATTCCATCCAAAGTTATTCTGGGACCAATTTGTTAAGTACAACTGTAATTGGTTCAGTTGCGTTCCCACAATAAGCATGATTATGCTGAACATGCCCAAACCAAACCCTTTCCCACACATTAGATTCATCAGATCGTGTTCTTCTGCTTTGGCTCCAGCAACGTTCCATAAGCTGGAGAAGGAATTCAATGCACCTGTCTTGGAGGCCTATGCGATGACCGAAGCATCACATCAAATGACCTCAAACAATCTGCCTCCAGGAAAGAGAAAGCCTGGTACTGTGGGCCAGCCACAAGGAGTCACCGTCGTCATTCTAGATGACAATGACAATGTCTTGCCCCCGGGCAAAGTCGGCGAAGTTTCCATCAGAGGCGAAAACGTCACTTTGGGGTATGCTAATAATCCAAAAGCTAACAAGGAGAACTTCACCAAGAGAGAGAACTATTTCAGAACCGGTGACCAAGGTTATTTCGACCCTGAGGGGTTTTTGGTCCTTACAGGCAGAATCAAAGAGCTTATCAACAGGGGTGGTGAAAAGATTTCACCCATTGAGCTCGACGGCATTATGCTATCGCATCCAAAGATCGATGAAGCCGTTGCATTTGGTGTTCCCGACGATATGTACGGCCAAGTAGTTCAAGCCGCCATTGTTTTGAAGAAGGGAGAAAAAATGACCTACGAAGAACTGGTGAACTTCTTAAAGAAGCACCTAGCCTCTTTCAAAATTCCAACCAAGGTGTACTTTGTTGATAAGCTACCAAAAACCGCTACAGGTAAAATCCAGAGAAGAGTTATCGCAGAAACTTTTGCTAAGAGCAGCAGAAATAAGAGTAAGTTGTAGGGTTCCTAGCTGATTAGAAGGCCATCCTGACGGATGGCCTTTTTTTTGACTCTGTTGTGTCTTCTTTTAGGTGTGGAATAATTTTCCAACCATCACTTTATCTGTCTCCCGAACAGCACCTTATTCAGCCATCGGTTCACATACACGTTCACGAAAGCACATCCTACTCCGATAGCGGCTCCTGCCACTACATCACTGGGATAGTGCACCCCTTGGTTCATCCGGGCAAACCCTACTCCGCAAGCCCATACGGCTGAAGGGGCGATAACATACCACTTAGGATAAGTGATGGAGAGTGAGGTGGCTAAAGAAAAAGCTGCCGCAGTGTGTCCTGAAGGGAATGACGGACTATCGGCATCCGGTGCTCCGTATGCATGAATCTTATCCGGATACTTTACAAAAGGCCGGTCACGACCTATTATATGTTTGGCGGCGTATGTAATCCCCACTGCTTCAATCACACTGGTTCCTATGTAAATGGCATCTTTCAGCATCGGCTGGTCTTTCTTTATCAATGCATAGAGTCCCATGGCTGTAGGTATTCCTACGGGTAGAATCACTCCGCTAGCGGACAATCCGCGGCTCAGTCCGTGTACATCCCATCGGTTGATGGTTTTCAACGTGTTGATGTCTATATTCTGTGCGCTGAGGCTGAATGTAAAACAGGCTGTCAGTATAAGAAAAATAATCCTTTGGCGTTTCATTTAGTTTGGCATTTGTTCAGTGCAAAGATAGGAAAATAATGTAATTCGCATTTACCCTTCCCCGAATTAAAGAATAATTTCCATAATTCCTTGTATTATCGCTAGAATTCCTTTACTTTTGCATCGTGAATACACAGCTTTTAACAGAATTTTTAGTTCTAACAATTAAATAATTTAAATTCAATCATTTATGTGGTTAAGTAATTCATCTATTGGAAGGAAAGTGGTGATGAGTGTCACAGGTATCGCCCTTGTCCTGTTTCTGACGTTTCACATGGCGATGAACCTTGTTGCGCTCTTCTCGGAAGATGCTTACAACATGATTTGTGAATTCTTGGGAGCTAACTGGTACGCGTTGGTTGCAACTGCCGGGCTGGCTGCTCTGTTTGTAATTCACATCATCTACGCTTTCTGGTTGACCATGCAGAACCGTGCAGCTCGCGGTAGCGAACGTTATGCAGTGAACGTTAAACCTAAAACTGTGGAATGGGCATCTCAGAACATGCTTGTCCTGGGTATTGTCGTTATTGCCGGATTGGCTCTTCATTTTGCCAATTTCTGGTACAAAATGCAGTTTGCCGAAATTATCGGTGTTCATGATTTGGGCGCATTCGGTCCTACAGACGGTGCCGCTTACATTCGTGAAACATTCGGATGCCCTGTATTTACAGTGCTTTACCTCATTTGGTTAGCAGCTTTGTGGTTCCACCTGACTCACGGATTCTGGAGTGCTTTGCAGACTTTGGGCTGGAGCAATAAAATCTGGTTCGAGCGTTGGAAGACTATTGGTAACATCTACTCTACTTTGGTAGTTCTTGGTTTTGCAGCAGTAGTCGTTATCTACTTCGCTATGACTGGCGCGCCTGATAGGTGGGCTGCCCTTCCTGGTTGGCTTGGTTTCATCAGCCATCCGCTTGCCCTCATCTGTTACGCCGGCGGTAGCCGGCCAGCCTCGCAGAGCAGGATTCCCGTTGAGCACCGCCAGGTGCGAATAAGGGACAGTGAAGAAGGAACACCCGCTCGCGGGTGGGCCTACTTCACCTATCCTGCCCGGCTGACGCCGTTGGATACACCAAGGAAAGTCTACACGAACCCTTTGGCAAAATCCTGTATATCGTGCGAAAAAGGATGGATATACCGAAAAAATCGCTATAATGACCCCGAAGCAGGGTTATGCAGCGGAAAAGCGGGATTAAAAGTCGGGGATTGGTGAACAAAAAGGTGTTTCTCTCTTTAAGAGAAATATCGTTTTGCTAAACAGTTGATATTGAGGTATCATTTTATCGTAAAAGACATTTTTGCTCAACAATTGCTTGACGGAAATCAACAAATTTTAGCATTTTGTAAAAAAGTCGCTATATAATTTGGTGAATTGGAGTTATTTTCATATTTTTGCATCCCGAAGAGTTTCTCTTAAAGAGAGAAACATCTTTTGCATACCTTTTCCGACCGAATTTTTATGTCGTAAAGAGGGGCTTTGCAGGGGGTGGACTCAGAAAGATGAGAATAGATGACTATTGTAGTTGAAACACATAGAAAGTTGCTGATATACAGACCGATACGCATATCGGGATGAACCATGAGTACGTTCTTTTCTCAAAAAACATAAATATTCGAAAAGAGATGCAATAAATTAAGGAGAGGTTATAATGAACAAAGTAAATATAAAAGATAGTCAAAATTTTATTACTTCAAAATATCACATAGAAAAAATAATGAATTGCATAAGTTTAGATGAAAAAGATAACATCTTTGAAATAGGTGCAGGGAAAGGTCATTTTACTGCTGGATTGGTAAAGAGATGTAATTTTGTAACGGCGATAGAAATTGATTCTAAATTATGTGAGGTAACTCGTAATAAGCTCTTAAATTATCCTAACTATCAAATAGTAAATGATGATATACTGAAATTTACATTTCCTAGCCACAATCCATATAAAATATTTGGCAGCATACCTTACAACATAAGCACAAATATAATTCGAAAAATTGTTTTTGAAAGTTCAGCCACAATAAGTTATTTAATAGTGGAATATGGTTTTGCTAAAATGTTATTAGATACAAACAGATCACTAGCATTGCTGTTAATGGCAGAGGTAGATATTTCTATATTAGCAAAAATTCCTAGGTATTATTTCCATCCAAAACCTAAAGTGGATAGCACATTAATTGTATTAAAAAGAAAGCCAGCAAAAATGGCATTTAAAGAGAGAAAAAAATATGAAACTTTTGTAATGAAATGGGTTAACAAAGAGTACGAAAAACTGTTTACAAAAAATCAATTTAATAAAGCTTTAAAACATGCGAGAATATATGATATAAACAATATTAGTTTCGAACAATTTGTATCGCTATTTAATAGTTATAAAATATTTAACGGCTAAAAACAATAGGCCACATGCAACTGTAAATGTTTACGCGGGTACCGACACCGCGGTGGAGGGGAATTGTGTTACAACCAATTAACCAATTCTGATTAGAAAAACTCATCGAGCATCAAATGAAACTGCAATTTATTCATATCAGGATTATCAATACCATATTTTTGAAAAAGCCGTTTCTGTAATGAAGGAGAAAACTCACCGAGGCAGTTCCATAGGATGGCAAGATCCTGGTATCGGTCTGCGATTCCGACTCGTCCAACATCAATACAACCTATTAATTTCCCCTCGTCAAAAATAAGGTTATCAAGTGAGAAATCACCATGAGTGACGACTGAATCCGGTGAGAATGGCAAAAGCTTATGCATTTCTTTCCAGACTTGTTCAACAGGCCAGCCATTACGCTCGTCATCAAAATCACTCGCATCAACCAAACCGTTATTCATTCGTGATTGCGCCTGAGCGAGGCGAAATACGCGATCGCTGTTAAAAGGACAATTACAAACAGGAATCGAATGCAACCGGCGCAGGAACACTGCCAGCGCATCAACAATATTTTCACCTGAATCAGGATATTCTTCTAATACCTGGAATGCTGTTTTCCCGGGGATCGCAGTGGTGAGTAACCATGCATCATCAGGAGTACGGATAAAATGCTTGATGGTCGGAAGAGGCATAAATTCCGTCAGCCAGTTTAGTCTGACCATCTCATCTGTAACATCATTGGCAACGCTACCTTTGCCATGTTTCAGAAACAACTCTGGCGCATCGGGCTTCCCATACAATCGATAGATTGTCGCACCTGATTGCCCGACATTATCGCGAGCCCATTTATACCCATATAAATCAGCATCCATGTTGGAATTTAATCGCGGCCTGGAGCAAGACGTTTCCCGTTGAATATGGCTCATAACACCCCTTGTATTACTGTTTATGTAAGCAGACAGTTTTATTGTTCATGATGATATATTTTTATCTTGTGCAATGTAACATCAGAGATTTTGAGACACAACGTGGCTTTGTTGAATAAATCGAACTTTTGCTGAGTTGAAGGATCAGAGCCTACGTTCCGAATACGGTCAAAAAAAAGGCCATCCGTCAGGATGGCCTTCGCATTAATATGCCGCTTCGAATTCTTTTAGGAAGCGTGTATCGTTTTCAGAGAACATACGGAGGTCTTTCACCTGATATTTCAGGTTTGTGATACGCTCGATACCCATACCGAGTCCATAACCGCTGTATATTTTGCTGTCTATACCATTTGATTCAAGTACGTTCGGGTCTACCATACCGCAACCGAGGATTTCTACCCAGCCGGTGTGTTTACAGAACGGACATCCTTTACCGCCGCAGATATTACAGCTGATATCCATTTCCGCACTTGGTTCAGCAAACGGGAAGTAAGACGGACGCAGACGGATCTTTGTATCAGCACCGAACATTTCTTTGGCAAAGAGCAGCAATACCTGCTTCAAGTCGGTGAATGATACGTTTTTATCTACATACAGCGCTTCTACCTGATGGAAGAAACAGTGTGCGCGATAGCTGATAGCTTCGTTACGATATACACGTCCCGGACAGATGATGCGGATAGGAGGCTGTGAAGTTTCCATCACACGAGTCTGTACAGAAGAAGTATGTGTACGCAATACTACGTCCGGGTGAGCTTCGATAAAGAAAGTGTCCTGCATATCGCGTGCCGGATGATCTTCGGCAAAGTTCAGTGCCGAGAACACGTGCCAGTCATCTTCAATTTCCGGACCTTCGGCAATGCTGAATCCCAGACGGGCAAAGATATCAATGATTTCGTTCTTTACAATGGTGAGCGGGTGGCGTGTACCGAGTTCTACAGGATAAGCCGAACGCGTCAAATCCAGTCCGTCACAATCGTTGTCCTGACTTTCAAACATTTCTTTCAGCGCGTTGATTTTGTCCTGCGCTTTTGTTTTCAGTTCATTCAGTCTCATGCCGACTTCTTTTTTCTGTTCGGCAGCTACATTACGGAAATCTGCCATTAAGTCGTTAATGGCTCCCTTCTTACTTAGGTATTTGATGCGGAGAGCTTCGAGTTCTTCGGCATTGGAGGCGTGTAAGGCTTCCACCTCTTTCAGAAGTTGTTCAATCTTAGCTATCATTTTTTAATATTTTTAGCGGCCCCGTTAAACAAAATTATTTGTAGAGGCTGTTTCGTCCTCACGGACTCATCAGACCGGAAAGCACATCCGGTGACAGCTCAGGCTACTTTGTTTCTTTCGACACTGCAAATATAAGAACATTATTTGAAAGTTCAAGTGAAACTTTAAATTTTAACAATAGATTAACCATTGCAAACAAAACAAAAAAAAGGTAGCCCAATTGTAAAACGAAAGGCCCAGTCTTTCGACTGAGCCTTTCGTTTTATCCTACAGTCGCTCGGCGATCGAAGGCTTCGGAAAAAAAAGGCCATCCGTCAGGATGGCCTTCGCATTAATATGCCGCTTCGAATTCTTTTAGGAAGCGTGTATCGTTTTCAGAGAACATACGGAGGTCTTTCACCTGATATTTCAGGTTTGTGATACGCTCGATACCCATACCGAGTCCATAACCGCTGTATATTTTGCTGTCTATACCATTTGATTCAAGTACGTTCGGGTCTACCATACCGCAACCGAGGATTTCTACCCAGCCGGTGTGTTTACAGAACGGACATCCTTTACCGCCGCAGATATTACAGCTGATATCCATTTCCGCACTTGGTTCAGCAAACGGGAAGTAAGACGGACGCAGACGGATCTTTGTATCAGCACCGAACATTTCTTTGGCAAAGAGCAGCAATACCTGCTTCAAGTCGGTGAATGATACGTTTTTATCTACATACAGCGCTTCTACCTGATGGAAGAAACAGTGTGCGCGATAGCTGATAGCTTCGTTACGATATACACGTCCCGGACAGATGATGCGGATAGGAGGCTGTGAAGTTTCCATCACACGAGTCTGTACAGAAGAAGTATGTGTACGCAATACTACGTCCGGGTGAGCTTCGATAAAGAAAGTGTCCTGCATATCGCGTGCCGGATGATCTTCGGCAAAGTTCAGTGCCGAGAACACGTGCCAGTCATCTTCAATTTCCGGACCTTCGGCAATGCTGAATCCCAGACGGGCAAAGATATCAATGATTTCGTTCTTTACAATGGTGAGCGGGTGGCGTGTACCGAGTTCTACAGGATAAGCCGAACGCGTCAAATCCAGTCCGTCACAATCGTTGTCCTGACTTTCAAACATTTCTTTCAGCGCGTTGATTTTGTCCTGCGCTTTTGTTTTCAGTTCATTCAGTCTCATGCCGACTTCTTTTTTCTGTTCGGCAGCTACATTACGGAAATCTGCCATTAAGTCGTTAATGGCTCCCTTCTTACTTAGGTATTTGATGCGGAGAGCTTCGAGTTCTTCGGCATTGGAGGCGTGTAAGGCTTCCACCTCTTTCAGAAGTTGTTCAATCTTAGCTATCATTTTTTAATATTTTTAGCGGCCCCGTTAAACAAAATTATTTGTAGAGGCTGTTTCGTCCTCACGGACTCATCAGACCGGAAAGCACATCCGGTGACAGCTCAGGCTACTTTGTTTCTTTCGACACTGCAAATATAAGAACATTATTTGAAAGTTCAAGTGAAACTTTAAATTTTAACAATAGATTAACCATTGCAAACAAAACAAAAAAAAGGTAGCCCAATTGTAAAACGAAAGGCCCAGTCTTTCGACTGAGCCTTTCGTTTTATCCTAGGATCAGCTGTACGTACTCGCAGTTCAACCTGTTGATAGTACGTACTAAGCTCTCATGTTTCACGTACTAAGCTCTCATGTTTAACGTACTAAGCTCTCATGTTTAACGAACTAAACCCTCATGGCTAACGTACTAAGCTCTCATGGCTAACGTACTAAGCTCTCATGTTTCACGTACTAAGCTCTCATGTTTGAACAATAAAATTAATATAAATCAGCAACTTAAATAGCCTCTAAGGTTTTAAGTTTTATAAGAAAAAAAAGAATATATAAGGCTTTTAAAGCTTTTAAGGTTTAACGGTTGTGGACAACAAGCCAGGGATGTAACGCACTGAGAAGCCCTTAGAGCCTCTCAAAGCAATTTTGAGTGACACAGGAACACTTAACGGCTGACATGGGGCGGCCGCGTAG
SEQ ID NO:83(用于基因组整合进普通拟杆菌基因组中的编码草酸盐降解途径的质粒):
GGAGAGCTGTATGTGGGGTGGAAGAAGCTATCGTAAAAATGCTGGAGCAGCTTGATCCTCATTCTACCTATTCTGATCCGGAGGAAGTGAAGAGAATGAACGAGCCGTTGCAGGGAAACTTCGAAGGTATCGGTATACAGTTTAATATGGCGGAGGACACTTTGCTGGTGATACAGCCTGTATCGGGCGGACCGTCCGAAAAAGTCGGTATTCTGGCCGGTGATCGTATCGTCATGGTGGAAGATACGCTGATAGCCGGTGTAAAGATGAGTACCGAGGATATTATGCGTCGCCTCCGTGGTCCTAAAGATTCAAAAGTGAATCTGAAAATACTTCGCCGAGGGGTGAAGGAACTGCTCCCCTTTACGGTGAAAAGGGATAAAATACCTGTTTACAGCTTGGATGCTTCCTATATGATTAAAGATAAGATCGGTTATATTCGTATCAACCGTTTTGCCGCCACTACCCATGAGGAGTTCAAGAAAGCGCTGACCGGGTTGCAGAAGAAAGGAATGAAAGACTTGATTCTTGATTTGCAGGGCAATGGCGGGGGGTATTTGAATGCTGCTATCGATATTGCCAATGAATTTTTGGGTAAAAAGGAACTGATTGTCTATACGGAGGGCCGTCGTAATCCTCGTAGCGAGTTTTTTGCCAAAGGTACGGGCGAGTTTCAGAACGGTCGTCTGATGGTATTGGTTGATGAATTTTCGGCTTCTGCCAGTGAGATTGTGACCGGAGCTGTTCAGGATTGGGACAGAGGCGTTGTGGTAGGCCGTCGCAGTTTTGGTAAAGGCTTGGTGCAGCGTCCCATTGATTTGCCGGACGGTTCGATGATCCGTCTGACGGTGGCCCGCTATTATACGCCTGCCGGACGTTGTATCCAGAAACCTTACGAGAGCATTGAAAAGTATAATGAAGACCTGATAGACCGTTATAATAAAGGAGAAATGATGAGTGAAGACAGCATCCACTTTCCGGATTCACTGAAGTTCAAGACTCATAGGTTGGCCCGTACGGTATATGGTGGCGGAGGGATCATGCCCGATTATTTTGTGCCGATTGATACGACTCTCTATACTAAATACCATCGTCAGTTGCGTGATAAAGGAGCATTGATGAAAGCTCATTTCCATTTTATTGATGCCCATCGTAAAGAATGGCTGGGCAAATACAAGACTTTCAATGAGTTCTACAAACGTTTTGAAGTGACTCCGGATATGTTGACACAACTGGTTGCTACCGGCAAGGAGATGGGAGTGGAATATAATGAAGAAGAGTATCAAAAGGCCCTGCCTCTGCTGAGGTTGCAGATGAAAGCATTGATAGCCCGTGATTTATGGGATATGAATGAATATTATCACGTCATTAATGACGCGAATGAAAGTATCAGGAAAGCGCTGGAACTGTTGGAGCAACCGGACTTTGAGGGGCTGCTGTTGAAAAAGCGATAGTCAAGACTTTATATTAACTGCAGGGGCGCATAGTTGCACTCCTGCAGTTGTCTGAAAAACTTGTAGGATAAAACGAAAGGCTCAGTCGAAAGACTGGGCCTTTCGTTTTACAATTGGGCTACCTTTTTTTTGTTTTGTTTGCAATGGTTAATCTATTGTTAAAATTTAAAGTTTCACTTGAACTTTCAAATAATGTTCTTATATTTGCAGTGTCGAAAGAAACAAAGTAGCCTGGCATCATCTACCTTCATTATTAAACAAACCAAATTAATATCATAAAAAAATAAAAAAATGACTACCACAGCAACGCAGCATTTTGCCCAGTTACTCCAGAAGTACGGAATTGATACTGTCTTCGGCATAGTAGGTATTCCGATCGTGCAGTTGGCCGACACTATGGTCGCGAATGGAATTAAGTTTATACCGTGTCGCAATGAGCAAGCCGCAAGTTACGCGGCAAGCGCCTACGGCTACATCTCGGACAAACCTGGTGTCCTGTTAATCGTGGGAGGGCCTGGCCTTATTCATGCCTTAGCGGGGATCTATAATAGTATGTCGAACCGCTGGCCCTTGTTGGTCATCGCCGGATCCAGCAGCCAGTCAGACATCCACAAGGGAGGTTTCCAGGAACTTGACCAGGTGTCCTTGCTAAGTCCGTTCCTAAAATTCACTGGTAAACTGACGCCGGATAACATCGATATGATAACCCAAAAAGCACTCAACTATTGCATTCAGGGAACCGCCGGAGTGAGCTATATCGATGTCCCCGCTGACTTCATCGAATACGAGAAGCCGCTTGAGGGCAATGATAGAACTGGTAACGAGCTGCCAATGATCCTGACGCCCAATATCTGCGGTCCAGACCCCAGTAAAATTAAGAAGGTGGTGCAATTGATACTCCAGCACAAGAACAAGAACATATTAATAGTGATAGGTAAAGGAGCAGTGAAGAATAGCCATGAGATTAGACGCTTGGTTAACACCTTTAACTTGCCGTTCCTTCCGACTCCCATGGCGAAAGGCATAGTGCCCGATAGCAGCCCTTTAAATGTGAGCAGCGCCCGGAGCCAGGCTCTTAAGATTGCTGACATCGTGTTAGTATTGGGAGCTCGCCTGAATTGGATCCTTCACTTCGGAACGAGTCCAAAGTGGAATAGCGAGAGTATTTTTATACAATTTGACAGTAACCCGGAAACGCTCGGAGACAATAACGTTAGTCCCGGAGCAGATTTAAGTATCTGGGGGGACATCGGACTATCGGTTACTGCACTGGTAGAAGAGCTCACGAGACAGGACAGTTGTTGGAAATACTCGGGAGTCAAACAGGAGATCAGGGAAAAGATACAGTTAAACCAGACCAGATTGCTCCGTAAGGAAAAAACGCGTGGAGCTCAGCTTAACTATAATCAAGTGTACGGCACCCTTCGTCCGTTAATTGACGACTATCGCACGATCTTGGTCACAGAAGGTGCCAACACAATGGACATCGCTAGAATAAGTTTTCCGACTGATGCACCTCGCAGAAGACTTGATGCTGGTACAAATGCTACAATGGGTATAGGTTTAGGTTACGCATTAGCGTGTAAAGCCAGCCACCCTGAGTTAGACGTTGTTTTAATCCAAGGCGACAGTGCCTTCGGTTTTAGCGCTATGGAGATCGAGACTGCCGTCCGTTGCCAATTAGCCCTTGTGATTGTCGTAATGAATAACTCTGGGATATATCACGGTGAGAAAGACATCGAAGGAGATCTGCCCCCGACCGCTTTGTCAAAAAATTGTAGATATGACTTGGTCGGTAAGGGTTTAGGAGCTAACGACTTCTTCGTCAATACCATCTCAGAGTTATCTCGCTGTTTTCAGCAGGCAGTTCAATTGAGCAGAACCAAGCGCGAAACCTCCGTCATTAATGTCATAATCGAACCGGGAGAGCAGAAACAGATCGCGTTCGCATGGCAAAACAAGCCTAGACTGTAAGGTTCCTAACCTGCCTGGCATCTTAATCAATTTTAAACTCACCAAATCAAAATATGAAAAAAAAAAATAATAAAATGTCAACTCCACTTCAAGGAATTAAAGTTCTCGATTTCACCGGTGTGCAATCTGGCCCATCTTGTACTCAAATGCTGGCCTGGTTTGGCGCTGACGTTATTAAAATTGAACGTCCCGGCGTTGGTGACGTAACGCGTCACCAGCTGCGAGATATTCCTGATATCGATGCGCTTTACTTCACCATGCTTAACAGTAACAAACGTTCTATTGAGTTAAATACCAAAACAGCGGAAGGCAAAGAGGTAATGGAAAAGCTGATCCGCGAAGCTGATATCTTAGTCGAGAACTTTCATCCAGGGGCCATTGATCACATGGGCTTCACCTGGGAGCATATTCAAGAAATCAATCCACGTCTGATTTTTGGTTCGATCAAAGGGTTTGATGAGTGTTCGCCTTATGTGAATGTAAAAGCCTATGAAAACGTTGCTCAGGCAGCGGGTGGCGCGGCATCCACTACGGGTTTTTGGGATGGTCCGCCGCTGGTAAGCGCTGCAGCGTTGGGTGACAGCAACACCGGAATGCATTTGCTGATCGGTTTACTTGCTGCTTTGCTGCATCGCGAAAAAACGGGGCGTGGGCAACGAGTCACCATGTCAATGCAGGATGCCGTATTGAACCTTTGCCGCGTGAAATTACGTGACCAGCAGCGTCTAGATAAATTGGGTTATCTGGAAGAATACCCGCAGTATCCGAATGGTACATTTGGTGATGCAGTTCCCCGCGGTGGTAATGCAGGTGGTGGCGGTCAGCCTGGCTGGATCCTGAAATGTAAAGGCTGGGAAACCGATCCTAACGCCTATATTTATTTCACTATTCAGGAGCAAAACTGGGAAAACACCTGTAAAGCCATCGGCAAACCAGAATGGATTACCGATCCGGCATACAGTACAGCCCATGCACGACAGCCACATATTTTCGATATTTTTGCTGAAATCGAAAAATACACTGTCACTATTGATAAACATGAAGCGGTGGCCTATTTGACTCAGTTTGATATTCCTTGTGCACCGGTTTTAAGTATGAAAGAAATTTCACTTGATCCCTCTTTGCGCCAAAGTGGCAGTGTTGTTGAAGTGGAACAACCGTTGCGTGGAAAATATCTGACCGTTGGTTGTCCAATGAAATTCTCTGCCTTTACGCCGGATATTAAAGCTGCGCCGCTATTAGGTGAACATACCGCTGCTGTATTGCAGGAGCTGGGTTATAGCGACGATGAAATTGCTGCAATGAAGCAAAACCACGCCATCTGAGGTTCCTAAACGACCTGGCATCATCTACCTTTATTAATCAACAATCCAAATTATTAATAAAAAAAATTTAAAAAATGACAAATAATGAAAGCAAAGGGCCGTTTGAAGGCTTATTAGTTATCGATATGACACATGTCCTTAATGGACCTTTCGGAACTCAACTTCTTTGTAATATGGGCGCAAGGGTAATTAAAGTTGAGCCGCCGGGTCATGGTGATGATACCCGCACATTTGGTCCCTATGTGGATGGACAGTCACTCTATTACAGTTTTATTAATCATGGCAAAGAGAGTGTGGTTCTTGATTTAAAGAATGATCACGATAAAAGTATATTTATAAATATGCTCAAACAAGCTGATGTATTAGCTGAGAATTTTCGCCCAGGTACAATGGAAAAACTGGGGTTTTCATGGGAAACGCTTCAAGAAATCAACCCGCGCCTCATATATGCTTCATCGTCAGGTTTCGGACATACCGGTCCGCTAAAAGATGCTCCTGCCTACGATACCATCATTCAGGCAATGAGCGGGATAATGATGGAAACAGGATATCCTGATGCTCCGCCAGTGCGCGTTGGTACATCTCTTGCGGATCTATGCGGCGGTGTCTATTTATTCAGCGGAATAGTGAGTGCACTTTATGGCCGCGAAAAGAGCCAGAGAGGGGCGCATGTCGATATAGCGATGTTTGATGCCACGCTGAGTTTTCTGGAGCATGGTCTGATGGCATATATCGCAACTGGGAAGTCACCACAACGTCTGGGAAATCGCCATCCCTACATGGCACCTTTTGATGTTTTCAATACTCAGGATAAGCCGATTACGATTTGTTGTGGTAATGACAAGCTTTTTTCTGCGTTATGCCAGGCACTGGAGCTTACGGAACTGGTTAATGATCCCCGATTTAGCAGCAATATTTTACGCGTACAAAACCAGGCTATTCTTAAACAATATATTGAGCGGACGTTAAAAACGCAGGCAGCTGAAGTTTGGTTAGCCAGAATACATGAAGTTGGTGTACCCGTCGCGCCGTTATTAAGTGTGGCTGAGGCCATTAAATTGCCACAAACTCAGGCGAGAAATATGTTGATTGAAGCCGGGGGAATAATGATGCCGGGTAATCCGATAAAAATCAGCGGCTGCGCGGACCCGCATGTTATGCCGGGAGCGGCAACGCTCGACCAGCATGGGGAACAAATTCGCCAGGAGTTCTCATCATAAGGTTCCTAAGGGTCCTGGCATCAACTACCTACATTAATAACCAACCCAAATGCCTAATCAATAAATATTATAAAATGAATAATCCACAAACAGGACAATCAACAGGCCTCTTGGGCAATCGTTGGTTCTACTTGGTATTAGCAGTTTTGCTGATGTGTATGATCTCGGGTGTCCAATATTCCTGGACACTGTACGCTAACCCGGTTAAAGACAACCTTGGCGTTTCTTTGGCTGCGGTTCAGACGGCTTTCACACTCTCTCAGGTCATTCAAGCTGGTTCTCAGCCTGGTGGTGGTTACTTCGTTGATAAATTCGGTCCAAGAATTCCATTGATGTTCGGTGGTGCGATGGTTCTCGCTGGCTGGACCTTCATGGGTATGGTTGACAGTGTTCCTGCTCTGTATGCTCTTTATACTCTGGCCGGTGCAGGTGTTGGTATCGTTTACGGTATCGCGATGAACACGGCTAACAGATGGTTCCCGGACAAACGCGGTCTGGCTTCCGGTTTCACCGCTGCCGGTTACGGTCTGGGTGTTCTGCCGTTCCTGCCACTGATCAGCTCCGTTCTGAAAGTTGAAGGTGTTGGCGCAGCATTCATGTACACCGGTTTGATCATGGGTATCCTGATTATCCTGATCGCTTTCGTTATCCGTTTCCCTGGCCAGCAAGGCGCCAAAAAACAAATCGTTGTTACCGACAAGGATTTCAATTCTGGCGAAATGCTGAGAACACCACAATTCTGGGTTCTGTGGACCGCATTCTTTTCCGTTAACTTTGGTGGTTTGCTGCTGGTTGCCAACAGCGTCCCTTACGGTCGCAGCCTCGGTCTTGCCGCAGGTGTGCTGACGATCGGTGTTTCGATCCAGAACCTGTTCAATGGTGGTTGCCGTCCTTTCTGGGGTTTCGTTTCCGATAAAATCGGCCGTTACAAAACCATGTCCGTCGTTTTCGGTATCAATGCTGTTGTTCTCGCACTTTTCCCGACGATTGCTGCCTTGGGCGATGTAGCCTTTATCGCCATGTTGGCAATCGCATTCTTCACATGGGGTGGTAGCTACGCTCTGTTCCCATCGACCAACAGCGATATTTTCGGTACGGCATACTCTGCCAGAAACTATGGTTTCTTCTGGGCTGCAAAAGCAACTGCCTCGATCTTCGGTGGTGGTCTGGGTGCTGCAATTGCAACCAACTTCGGATGGAATACCGCTTTCCTGATTACTGCGATTACTTCTTTCATCGCATTTGCTCTGGCTACCTTCGTTATTCCAAGAATGGGCCGTCCAGTCAAGAAAATGGTCAAATTGTCTCCAGAAGAAAAAGCTGTACATTAAGGTTCCTAATTCCCCTGGCATCCCCAAAAAATTAATTCATTTTCTCTTTTCAACAATTTAAAAAAAATTAAAAAATGAGTAACGACGACAATGTAGAGTTGACTGATGGCTTTCATGTTTTGATCGATGCCCTGAAAATGAATGACATCGATACCATGTATGGTGTTGTCGGCATTCCTATCACGAACCTGGCTCGTATGTGGCAAGATGACGGTCAGCGTTTTTACAGCTTCCGTCACGAACAACACGCAGGTTATGCAGCTTCTATCGCCGGTTACATCGAAGGAAAACCTGGCGTTTGCTTGACCGTTTCCGCCCCTGGCTTCCTGAACGGCGTGACTTCCCTGGCTCATGCAACCACCAACTGCTTCCCAATGATCCTGTTGAGCGGTTCCAGTGAACGTGAAATCGTCGATTTGCAACAGGGCGATTACGAAGAAATGGATCAGATGAATGTTGCACGTCCACACTGCAAAGCTTCTTTCCGTATCAACAGCATCAAAGACATTCCAATCGGTATCGCTCGTGCAGTTCGCACCGCTGTATCCGGACGTCCAGGTGGTGTTTACGTTGACTTGCCAGCAAAACTGTTCGGTCAGACCATTTCTGTAGAAGAAGCTAACAAACTGCTCTTCAAACCAATCGATCCAGCTCCGGCACAGATTCCTGCTGAAGATGCTATCGCTCGCGCTGCTGACCTGATCAAGAACGCCAAACGTCCAGTTATCATGCTGGGTAAAGGCGCTGCATACGCACAATGCGACGACGAAATCCGCGCACTGGTTGAAGAAACCGGCATCCCATTCCTGCCAATGGGTATGGCTAAAGGCCTGCTGCCTGACAACCATCCACAATCCGCTGCTGCAACCCGTGCTTTCGCACTGGCACAGTGTGACGTTTGCGTACTGATCGGCGCTCGTCTGAACTGGCTGATGCAGCACGGTAAAGGCAAAACCTGGGGCGACGAACTGAAGAAATACGTTCAGATCGACATCCAGGCTAACGAAATGGACAGCAACCAGCCTATCGCTGCACCAGTTGTTGGTGACATCAAGTCCGCCGTTTCCCTGCTCCGCAAAGCACTGAAAGGCGCTCCAAAAGCTGACGCTGAATGGACCGGCGCTCTGAAAGCCAAAGTTGACGGCAACAAAGCCAAACTGGCTGGCAAGATGACTGCCGAAACCCCATCCGGAATGATGAACTACTCCAATTCCCTGGGCGTTGTTCGTGACTTCATGCTGGCAAATCCGGATATTTCCCTGGTTAACGAAGGCGCTAATGCACTCGACAACACTCGTATGATTGTTGACATGCTGAAACCACGCAAACGTCTTGACTCCGGTACCTGGGGTGTTATGGGTATTGGTATGGGCTACTGCGTTGCTGCAGCTGCTGTTACCGGCAAACCGGTTATCGCTGTTGAAGGCGATAGCGCATTCGGTTTCTCCGGTATGGAACTGGAAACCATCTGCCGTTACAACCTGCCAGTTACCGTTATCATCATGAACAATGGTGGTATCTATAAAGGTAACGAAGCAGATCCACAACCAGGCGTTATCTCCTGTACCCGTCTGACCCGTGGTCGTTACGACATGATGATGGAAGCATTTGGCGGTAAAGGTTATGTTGCCAATACTCCAGCAGAACTGAAAGCTGCTCTGGAAGAAGCTGTTGCTTCCGGCAAACCATGCCTGATCAACGCGATGATCGATCCAGACGCTGGTGTCGAATCTGGCCGTATCAAGAGCCTGAACGTTGTAAGTAAAGTTGGCAAGAAATAAGGTTCCTAAAATGCCTGGCATCTAAACTAAAATACAATAACCAAAAATCATCTATGCGTAAAAAAAATTATAAAATGGATAGCGATACTCTCTCAGGATTATTGGAAAACGTCGCCAAAAAATTCCCCGATCGCCGAGCTCTCTCCGTTTCTGGAAAATTCAATCTCACTCACGCGCGTCTTCACGATCTAATCGAACGCGCCGCTTCACGCCTTGTCTCCGACGCTGGAATCAAACCCGGCGATGTCGTTGCTCTCACCTTCCCTAACACCGTCGAGTTTGTTATAATGTTTTTAGCGGTGATAAGAGCTAGAGCCACGGCGGCGCCGTTGAACGCAGCGTACACGGCGGAGGAATTTGAGTTTTACCTCTCCGATTCAGATTCAAAGCTATTGTTAACCTCTAAAGAAGGAAACGCACCGGCTCAAGAAGCAGCTTCAAAGCTGAAAATCTCTCACGTCACCGCTACGCTGCTTGACGCTGGCTCGGACCTTGTACTATCCGTTGCGGATTCAGATTCCGTCGTTGACTCAGCGACGGAACTCGTTAATCACCCGGACGACGGTGCTCTCTTCCTCCACACTTCTGGCACTACGAGCCGTCCAAAGGGTGTACCGCTTACGCAGCTCAATCTAGCTTCATCCGTCAAGAACATTAAAGCTGTGTACAAGCTTACTGAGTCTGATTCTACGGTGATTGTTCTCCCTCTGTTCCATGTTCATGGATTGTTAGCTGGGTTGCTTAGCTCGCTTGGAGCTGGTGCTGCTGTAACTCTTCCAGCTGCTGGTAGATTCTCAGCAACAACATTTTGGCCAGATATGAAGAAGTATAACGCTACATGGTATACTGCTGTGCCGACCATTCATCAGATCATATTGGACCGCCACGCGAGCCACCCTGAAACGGAATATCCTAAACTCCGGTTCATCAGGAGTTGCAGTGCTTCTTTGGCTCCGGTGATATTGTCCAGGCTTGAGGAAGCGTTTGGAGCACCGGTGCTCGAGGCCTATGCAATGACAGAGGCAACACATTTGATGAGCTCAAACCCCTTACCAGAGGAAGGTCCACACAAGCCTGGGTCTGTTGGGAAACCGGTAGGTCAAGAAATGGCGATCCTTAATGAGAAAGGCGAGATCCAAGAGCCAAATAACAAAGGAGAGGTTTGTATAAGAGGTCCAAATGTGACCAAGGGTTACAAGAATAACCCAGAAGCCAACAAGGCAGGTTTCGAGTTTGGGTGGTTCCACACTGGTGATATCGGTTACTTTGATACCGATGGGTATTTGCATCTGGTGGGTCGGATCAAAGAGCTTATTAACCGTGGAGGTGAGAAGATATCTCCAATTGAAGTGGATGCAGTACTCTTAACGCATCCTGACGTTTCTCAGGGTGTTGCATTCGGTGTTCCTGATGAGAAATATGGGGAAGAGATTAACTGTGCGGTGATTCCAAGAGAAGGAACTACTGTAACCGAAGAGGACATTAAAGCGTTTTGTAAGAAGAATTTGGCAGCTTTCAAGGTGCCAAAGAGAGTGTTCATCACTGATAACCTCCCCAAAACTGCCTCTGGTAAGATTCAGCGCCGTATCGTCGCACAACATTTCCTTGAGAAGCCCTGAGGTTCCTAAAAACCCTGGCATCTTTTCCATAACAAAAATTAAACCTCCTCCATATGAAAAATAAAAATTATAAAATGACAAGTGCCGCTACTGTTACTGCTTCGTTCAACGATACTTTTAGCGTATCCGATAATGTCGCCGTTATTGTTCCTGAAACGGACACTCAGGTGACCTACAGGGATCTATCCCACATGGTGGGTCACTTCCAGACCATGTTCACAAATCCTAATTCTCCATTGTACGGAGCTGTTTTCAGACAAGATACAGTGGCGATATCCATGCGTAATGGGCTGGAATTTATCGTCGCTTTCCTCGGTGCTACTATGGACGCTAAAATTGGCGCGCCCTTGAATCCCAATTATAAGGAAAAGGAGTTCAATTTTTATTTGAATGACCTGAAATCTAAGGCGATTTGCGTCCCAAAGGGTACCACAAAGTTACAGAGTTCTGAAATTCTAAAATCTGCCTCCACGTTTGGATGTTTTATCGTAGAGCTGGCCTTCGATGCGACCAGGTTTAGGGTAGAGTATGATATATACTCTCCAGAGGACAACTACAAAAGGGTTATTTACCGGTCTTTGAACAACGCCAAATTTGTCAACACAAATCCCGTTAAATTCCCTGGGTTTGCCCGTTCCAGTGACGTTGCCCTGATTTTGCATACCAGTGGTACCACCTCCACTCCAAAAACGGTGCCTTTGTTACATTTGAACATTGTGAGAAGCACGTTGAACATTGCTAACACTTACAAGCTAACGCCCTTGGACAGATCTTATGTCGTGATGCCTCTTTTCCACGTCCATGGGTTAATTGGTGTTTTACTTTCCACTTTTAGAACTCAGGGTTCTGTTGTGGTTCCCGATGGATTCCATCCAAAGTTATTCTGGGACCAATTTGTTAAGTACAACTGTAATTGGTTCAGTTGCGTTCCCACAATAAGCATGATTATGCTGAACATGCCCAAACCAAACCCTTTCCCACACATTAGATTCATCAGATCGTGTTCTTCTGCTTTGGCTCCAGCAACGTTCCATAAGCTGGAGAAGGAATTCAATGCACCTGTCTTGGAGGCCTATGCGATGACCGAAGCATCACATCAAATGACCTCAAACAATCTGCCTCCAGGAAAGAGAAAGCCTGGTACTGTGGGCCAGCCACAAGGAGTCACCGTCGTCATTCTAGATGACAATGACAATGTCTTGCCCCCGGGCAAAGTCGGCGAAGTTTCCATCAGAGGCGAAAACGTCACTTTGGGGTATGCTAATAATCCAAAAGCTAACAAGGAGAACTTCACCAAGAGAGAGAACTATTTCAGAACCGGTGACCAAGGTTATTTCGACCCTGAGGGGTTTTTGGTCCTTACAGGCAGAATCAAAGAGCTTATCAACAGGGGTGGTGAAAAGATTTCACCCATTGAGCTCGACGGCATTATGCTATCGCATCCAAAGATCGATGAAGCCGTTGCATTTGGTGTTCCCGACGATATGTACGGCCAAGTAGTTCAAGCCGCCATTGTTTTGAAGAAGGGAGAAAAAATGACCTACGAAGAACTGGTGAACTTCTTAAAGAAGCACCTAGCCTCTTTCAAAATTCCAACCAAGGTGTACTTTGTTGATAAGCTACCAAAAACCGCTACAGGTAAAATCCAGAGAAGAGTTATCGCAGAAACTTTTGCTAAGAGCAGCAGAAATAAGAGTAAGTTGTAGGGTTCCTAGCTGATTAGAAGGCCATCCTGACGGATGGCCTTTTTTTTGACTCTGTTGTGTCTTCTTTTAGGTGTGGAATAATTTTCCAACCATCACTTTATCTGTCTCCCGAACAGCACCTTATTCAGCCATCGGTTCACATACACGTTCACGAAAGCACATCCTACTCCGATAGCGGCTCCTGCCACTACATCACTGGGATAGTGCACCCCTTGGTTCATCCGGGCAAACCCTACTCCGCAAGCCCATACGGCTGAAGGGGCGATAACATACCACTTAGGATAAGTGATGGAGAGTGAGGTGGCTAAAGAAAAAGCTGCCGCAGTGTGTCCTGAAGGGAATGACGGACTATCGGCATCCGGTGCTCCGTATGCATGAATCTTATCCGGATACTTTACAAAAGGCCGGTCACGACCTATTATATGTTTGGCGGCGTATGTAATCCCCACTGCTTCAATCACACTGGTTCCTATGTAAATGGCATCTTTCAGCATCGGCTGGTCTTTCTTTATCAATGCATAGAGTCCCATGGCTGTAGGTATTCCTACGGGTAGAATCACTCCGCTAGCGGACAATCCGCGGCTCAGTCCGTGTACATCCCATCGGTTGATGGTTTTCAACGTGTTGATGTCTATATTCTGTGCGCTGAGGCTGAATGTAAAACAGGCTGTCAGTATAAGAAAAATAATCCTTTGGCGTTTCATTTAGTTTGGCATTTGTTCAGTGCAAAGATAGGAAAATAATGTAATTCGCATTTACCCTTCCCCGAATTAAAGAATAATTTCCATAATTCCTTGTATTATCGCTAGAATTCCTTTACTTTTGCATCGTGAATACACAGCTTTTAACAGAATTTTTAGTTCTAACAATTAAATAATTTAAATTCAATCATTTATGTGGTTAAGTAATTCATCTATTGGAAGGAAAGTGGTGATGAGTGTCACAGGTATCGCCCTTGTCCTGTTTCTGACGTTTCACATGGCGATGAACCTTGTTGCGCTCTTCTCGGAAGATGCTTACAACATGATTTGTGAATTCTTGGGAGCTAACTGGTACGCGTTGGTTGCAACTGCCGGGCTGGCTGCTCTGTTTGTAATTCACATCATCTACGCTTTCTGGTTGACCATGCAGAACCGTGCAGCTCGCGGTAGCGAACGTTATGCAGTGAACGTTAAACCTAAAACTGTGGAATGGGCATCTCAGAACATGCTTGTCCTGGGTATTGTCGTTATTGCCGGATTGGCTCTTCATTTTGCCAATTTCTGGTACAAAATGCAGTTTGCCGAAATTATCGGTGTTCATGATTTGGGCGCATTCGGTCCTACAGACGGTGCCGCTTACATTCGTGAAACATTCGGATGCCCTGTATTTACAGTGCTTTACCTCATTTGGTTAGCAGCTTTGTGGTTCCACCTGACTCACGGATTCTGGAGTGCTTTGCAGACTTTGGGCTGGAGCAATAAAATCTGGTTCGAGCGTTGGAAGACTATTGGTAACATCTACTCTACTTTGGTAGTTCTTGGTTTTGCAGCAGTAGTCGTTATCTACTTCGCTATGACTGGCGCGCCTGATAGGTGGGCTGCCCTTCCTGGTTGGCTTGGTTTCATCAGCCATCCGCTTGCCCTCATCTGTTACGCCGGCGGTAGCCGGCCAGCCTCGCAGAGCAGGATTCCCGTTGAGCACCGCCAGGTGCGAATAAGGGACAGTGAAGAAGGAACACCCGCTCGCGGGTGGGCCTACTTCACCTATCCTGCCCGGCTGACGCCGTTGGATACACCAAGGAAAGTCTACACGAACCCTTTGGCAAAATCCTGTATATCGTGCGAAAAAGGATGGATATACCGAAAAAATCGCTATAATGACCCCGAAGCAGGGTTATGCAGCGGAAAAGCGGGATTAAAAGTCGGGGATTGGTGAACAAAAAGGTGTTTCTCTCTTTAAGAGAAATATCGTTTTGCTAAACAGTTGATATTGAGGTATCATTTTATCGTAAAAGACATTTTTGCTCAACAATTGCTTGACGGAAATCAACAAATTTTAGCATTTTGTAAAAAAGTCGCTATATAATTTGGTGAATTGGAGTTATTTTCATATTTTTGCATCCCGAAGAGTTTCTCTTAAAGAGAGAAACATCTTTTGCATACCTTTTCCGACCGAATTTTTATGTCGTAAAGAGGGGCTTTGCAGGGGGTGGACTCAGAAAGATGAGAATAGATGACTATTGTAGTTGAAACACATAGAAAGTTGCTGATATACAGACCGATACGCATATCGGGATGAACCATGAGTACGTTCTTTTCTCAAAAAACATAAATATTCGAAAAGAGATGCAATAAATTAAGGAGAGGTTATAATGAACAAAGTAAATATAAAAGATAGTCAAAATTTTATTACTTCAAAATATCACATAGAAAAAATAATGAATTGCATAAGTTTAGATGAAAAAGATAACATCTTTGAAATAGGTGCAGGGAAAGGTCATTTTACTGCTGGATTGGTAAAGAGATGTAATTTTGTAACGGCGATAGAAATTGATTCTAAATTATGTGAGGTAACTCGTAATAAGCTCTTAAATTATCCTAACTATCAAATAGTAAATGATGATATACTGAAATTTACATTTCCTAGCCACAATCCATATAAAATATTTGGCAGCATACCTTACAACATAAGCACAAATATAATTCGAAAAATTGTTTTTGAAAGTTCAGCCACAATAAGTTATTTAATAGTGGAATATGGTTTTGCTAAAATGTTATTAGATACAAACAGATCACTAGCATTGCTGTTAATGGCAGAGGTAGATATTTCTATATTAGCAAAAATTCCTAGGTATTATTTCCATCCAAAACCTAAAGTGGATAGCACATTAATTGTATTAAAAAGAAAGCCAGCAAAAATGGCATTTAAAGAGAGAAAAAAATATGAAACTTTTGTAATGAAATGGGTTAACAAAGAGTACGAAAAACTGTTTACAAAAAATCAATTTAATAAAGCTTTAAAACATGCGAGAATATATGATATAAACAATATTAGTTTCGAACAATTTGTATCGCTATTTAATAGTTATAAAATATTTAACGGCTAAAAACAATAGGCCACATGCAACTGTAAATGTTTACGCGGGTACCGACACCGCGGTGGAGGGGAATTGTGTTACAACCAATTAACCAATTCTGATTAGAAAAACTCATCGAGCATCAAATGAAACTGCAATTTATTCATATCAGGATTATCAATACCATATTTTTGAAAAAGCCGTTTCTGTAATGAAGGAGAAAACTCACCGAGGCAGTTCCATAGGATGGCAAGATCCTGGTATCGGTCTGCGATTCCGACTCGTCCAACATCAATACAACCTATTAATTTCCCCTCGTCAAAAATAAGGTTATCAAGTGAGAAATCACCATGAGTGACGACTGAATCCGGTGAGAATGGCAAAAGCTTATGCATTTCTTTCCAGACTTGTTCAACAGGCCAGCCATTACGCTCGTCATCAAAATCACTCGCATCAACCAAACCGTTATTCATTCGTGATTGCGCCTGAGCGAGGCGAAATACGCGATCGCTGTTAAAAGGACAATTACAAACAGGAATCGAATGCAACCGGCGCAGGAACACTGCCAGCGCATCAACAATATTTTCACCTGAATCAGGATATTCTTCTAATACCTGGAATGCTGTTTTCCCGGGGATCGCAGTGGTGAGTAACCATGCATCATCAGGAGTACGGATAAAATGCTTGATGGTCGGAAGAGGCATAAATTCCGTCAGCCAGTTTAGTCTGACCATCTCATCTGTAACATCATTGGCAACGCTACCTTTGCCATGTTTCAGAAACAACTCTGGCGCATCGGGCTTCCCATACAATCGATAGATTGTCGCACCTGATTGCCCGACATTATCGCGAGCCCATTTATACCCATATAAATCAGCATCCATGTTGGAATTTAATCGCGGCCTGGAGCAAGACGTTTCCCGTTGAATATGGCTCATAACACCCCTTGTATTACTGTTTATGTAAGCAGACAGTTTTATTGTTCATGATGATATATTTTTATCTTGTGCAATGTAACATCAGAGATTTTGAGACACAACGTGGCTTTGTTGAATAAATCGAACTTTTGCTGAGTTGAAGGATCAGAGCCTACGTTCCGAATACGGTCAAAAAAAAGGCCATCCGTCAGGATGGCCTTCGCATTAATATGCCGCTTCGAATTCTTTTAGGAAGCGTGTATCGTTTTCAGAGAACATACGGAGGTCTTTCACCTGATATTTCAGGTTTGTGATACGCTCGATACCCATACCGAGTCCATAACCGCTGTATATTTTGCTGTCTATACCATTTGATTCAAGTACGTTCGGGTCTACCATACCGCAACCGAGGATTTCTACCCAGCCGGTGTGTTTACAGAACGGACATCCTTTACCGCCGCAGATATTACAGCTGATATCCATTTCCGCACTTGGTTCAGCAAACGGGAAGTAAGACGGACGCAGACGGATCTTTGTATCAGCACCGAACATTTCTTTGGCAAAGAGCAGCAATACCTGCTTCAAGTCGGTGAATGATACGTTTTTATCTACATACAGCGCTTCTACCTGATGGAAGAAACAGTGTGCGCGATAGCTGATAGCTTCGTTACGATATACACGTCCCGGACAGATGATGCGGATAGGAGGCTGTGAAGTTTCCATCACACGAGTCTGTACAGAAGAAGTATGTGTACGCAATACTACGTCCGGGTGAGCTTCGATAAAGAAAGTGTCCTGCATATCGCGTGCCGGATGATCTTCGGCAAAGTTCAGTGCCGAGAACACGTGCCAGTCATCTTCAATTTCCGGACCTTCGGCAATGCTGAATCCCAGACGGGCAAAGATATCAATGATTTCGTTCTTTACAATGGTGAGCGGGTGGCGTGTACCGAGTTCTACAGGATAAGCCGAACGCGTCAAATCCAGTCCGTCACAATCGTTGTCCTGACTTTCAAACATTTCTTTCAGCGCGTTGATTTTGTCCTGCGCTTTTGTTTTCAGTTCATTCAGTCTCATGCCGACTTCTTTTTTCTGTTCGGCAGCTACATTACGGAAATCTGCCATTAAGTCGTTAATGGCTCCCTTCTTACTTAGGTATTTGATGCGGAGAGCTTCGAGTTCTTCGGCATTGGAGGCGTGTAAGGCTTCCACCTCTTTCAGAAGTTGTTCAATCTTAGCTATCATTTTTTAATATTTTTAGCGGCCCCGTTAAACAAAATTATTTGTAGAGGCTGTTTCGTCCTCACGGACTCATCAGACCGGAAAGCACATCCGGTGACAGCTCAGGCTACTTTGTTTCTTTCGACACTGCAAATATAAGAACATTATTTGAAAGTTCAAGTGAAACTTTAAATTTTAACAATAGATTAACCATTGCAAACAAAACAAAAAAAAGGTAGCCCAATTGTAAAACGAAAGGCCCAGTCTTTCGACTGAGCCTTTCGTTTTATCCTACAGTCGCTCGGCGATCGAAGGCTTCGGAAAAAAAAGGCCATCCGTCAGGATGGCCTTCGCATTAATATGCCGCTTCGAATTCTTTTAGGAAGCGTGTATCGTTTTCAGAGAACATACGGAGGTCTTTCACCTGATATTTCAGGTTTGTGATACGCTCGATACCCATACCGAGTCCATAACCGCTGTATATTTTGCTGTCTATACCATTTGATTCAAGTACGTTCGGGTCTACCATACCGCAACCGAGGATTTCTACCCAGCCGGTGTGTTTACAGAACGGACATCCTTTACCGCCGCAGATATTACAGCTGATATCCATTTCCGCACTTGGTTCAGCAAACGGGAAGTAAGACGGACGCAGACGGATCTTTGTATCAGCACCGAACATTTCTTTGGCAAAGAGCAGCAATACCTGCTTCAAGTCGGTGAATGATACGTTTTTATCTACATACAGCGCTTCTACCTGATGGAAGAAACAGTGTGCGCGATAGCTGATAGCTTCGTTACGATATACACGTCCCGGACAGATGATGCGGATAGGAGGCTGTGAAGTTTCCATCACACGAGTCTGTACAGAAGAAGTATGTGTACGCAATACTACGTCCGGGTGAGCTTCGATAAAGAAAGTGTCCTGCATATCGCGTGCCGGATGATCTTCGGCAAAGTTCAGTGCCGAGAACACGTGCCAGTCATCTTCAATTTCCGGACCTTCGGCAATGCTGAATCCCAGACGGGCAAAGATATCAATGATTTCGTTCTTTACAATGGTGAGCGGGTGGCGTGTACCGAGTTCTACAGGATAAGCCGAACGCGTCAAATCCAGTCCGTCACAATCGTTGTCCTGACTTTCAAACATTTCTTTCAGCGCGTTGATTTTGTCCTGCGCTTTTGTTTTCAGTTCATTCAGTCTCATGCCGACTTCTTTTTTCTGTTCGGCAGCTACATTACGGAAATCTGCCATTAAGTCGTTAATGGCTCCCTTCTTACTTAGGTATTTGATGCGGAGAGCTTCGAGTTCTTCGGCATTGGAGGCGTGTAAGGCTTCCACCTCTTTCAGAAGTTGTTCAATCTTAGCTATCATTTTTTAATATTTTTAGCGGCCCCGTTAAACAAAATTATTTGTAGAGGCTGTTTCGTCCTCACGGACTCATCAGACCGGAAAGCACATCCGGTGACAGCTCAGGCTACTTTGTTTCTTTCGACACTGCAAATATAAGAACATTATTTGAAAGTTCAAGTGAAACTTTAAATTTTAACAATAGATTAACCATTGCAAACAAAACAAAAAAAAGGTAGCCCAATTGTAAAACGAAAGGCCCAGTCTTTCGACTGAGCCTTTCGTTTTATCCTAGGATCAGCTGTACGTACTCGCAGTTCAACCTGTTGATAGTACGTACTAAGCTCTCATGTTTCACGTACTAAGCTCTCATGTTTAACGTACTAAGCTCTCATGTTTAACGAACTAAACCCTCATGGCTAACGTACTAAGCTCTCATGGCTAACGTACTAAGCTCTCATGTTTCACGTACTAAGCTCTCATGTTTGAACAATAAAATTAATATAAATCAGCAACTTAAATAGCCTCTAAGGTTTTAAGTTTTATAAGAAAAAAAAGAATATATAAGGCTTTTAAAGCTTTTAAGGTTTAACGGTTGTGGACAACAAGCCAGGGATGTAACGCACTGAGAAGCCCTTAGAGCCTCTCAAAGCAATTTTGAGTGACACAGGAACACTTAACGGCTGACATGGGGCGGCCGCGTAG
SEQ ID NO:84(用于基因组整合进普通拟杆菌基因组中的编码草酸盐降解途径的质粒)：
GGAGAGCTGTATGTGGGGTGGAAGAAGCTATCGTAAAAATGCTGGAGCAGCTTGATCCTCATTCTACCTATTCTGATCCGGAGGAAGTGAAGAGAATGAACGAGCCGTTGCAGGGAAACTTCGAAGGTATCGGTATACAGTTTAATATGGCGGAGGACACTTTGCTGGTGATACAGCCTGTATCGGGCGGACCGTCCGAAAAAGTCGGTATTCTGGCCGGTGATCGTATCGTCATGGTGGAAGATACGCTGATAGCCGGTGTAAAGATGAGTACCGAGGATATTATGCGTCGCCTCCGTGGTCCTAAAGATTCAAAAGTGAATCTGAAAATACTTCGCCGAGGGGTGAAGGAACTGCTCCCCTTTACGGTGAAAAGGGATAAAATACCTGTTTACAGCTTGGATGCTTCCTATATGATTAAAGATAAGATCGGTTATATTCGTATCAACCGTTTTGCCGCCACTACCCATGAGGAGTTCAAGAAAGCGCTGACCGGGTTGCAGAAGAAAGGAATGAAAGACTTGATTCTTGATTTGCAGGGCAATGGCGGGGGGTATTTGAATGCTGCTATCGATATTGCCAATGAATTTTTGGGTAAAAAGGAACTGATTGTCTATACGGAGGGCCGTCGTAATCCTCGTAGCGAGTTTTTTGCCAAAGGTACGGGCGAGTTTCAGAACGGTCGTCTGATGGTATTGGTTGATGAATTTTCGGCTTCTGCCAGTGAGATTGTGACCGGAGCTGTTCAGGATTGGGACAGAGGCGTTGTGGTAGGCCGTCGCAGTTTTGGTAAAGGCTTGGTGCAGCGTCCCATTGATTTGCCGGACGGTTCGATGATCCGTCTGACGGTGGCCCGCTATTATACGCCTGCCGGACGTTGTATCCAGAAACCTTACGAGAGCATTGAAAAGTATAATGAAGACCTGATAGACCGTTATAATAAAGGAGAAATGATGAGTGAAGACAGCATCCACTTTCCGGATTCACTGAAGTTCAAGACTCATAGGTTGGCCCGTACGGTATATGGTGGCGGAGGGATCATGCCCGATTATTTTGTGCCGATTGATACGACTCTCTATACTAAATACCATCGTCAGTTGCGTGATAAAGGAGCATTGATGAAAGCTCATTTCCATTTTATTGATGCCCATCGTAAAGAATGGCTGGGCAAATACAAGACTTTCAATGAGTTCTACAAACGTTTTGAAGTGACTCCGGATATGTTGACACAACTGGTTGCTACCGGCAAGGAGATGGGAGTGGAATATAATGAAGAAGAGTATCAAAAGGCCCTGCCTCTGCTGAGGTTGCAGATGAAAGCATTGATAGCCCGTGATTTATGGGATATGAATGAATATTATCACGTCATTAATGACGCGAATGAAAGTATCAGGAAAGCGCTGGAACTGTTGGAGCAACCGGACTTTGAGGGGCTGCTGTTGAAAAAGCGATAGTCAAGACTTTATATTAACTGCAGGGGCGCATAGTTGCACTCCTGCAGTTGTCTGAAAAACTTGTAGGATAAAACGAAAGGCTCAGTCGAAAGACTGGGCCTTTCGTTTTACAATTGGGCTACCTTTTTTTTGTTTTGTTTGCAATGGTTAATCTATTGTTAAAATTTAAAGTTTCACTTGAACTTTCAAATAATGTTCTTATATTTGCAGTGTCGAAAGAAACAAAGTAGCCTGGCATCATCTACCTTCATTATTAAACAAACCAAATTAATATCATAAAAAAATAAAAAAATGACTACCACAGCAACGCAGCATTTTGCCCAGTTACTCCAGAAGTACGGAATTGATACTGTCTTCGGCATAGTAGGTATTCCGATCGTGCAGTTGGCCGACACTATGGTCGCGAATGGAATTAAGTTTATACCGTGTCGCAATGAGCAAGCCGCAAGTTACGCGGCAAGCGCCTACGGCTACATCTCGGACAAACCTGGTGTCCTGTTAATCGTGGGAGGGCCTGGCCTTATTCATGCCTTAGCGGGGATCTATAATAGTATGTCGAACCGCTGGCCCTTGTTGGTCATCGCCGGATCCAGCAGCCAGTCAGACATCCACAAGGGAGGTTTCCAGGAACTTGACCAGGTGTCCTTGCTAAGTCCGTTCCTAAAATTCACTGGTAAACTGACGCCGGATAACATCGATATGATAACCCAAAAAGCACTCAACTATTGCATTCAGGGAACCGCCGGAGTGAGCTATATCGATGTCCCCGCTGACTTCATCGAATACGAGAAGCCGCTTGAGGGCAATGATAGAACTGGTAACGAGCTGCCAATGATCCTGACGCCCAATATCTGCGGTCCAGACCCCAGTAAAATTAAGAAGGTGGTGCAATTGATACTCCAGCACAAGAACAAGAACATATTAATAGTGATAGGTAAAGGAGCAGTGAAGAATAGCCATGAGATTAGACGCTTGGTTAACACCTTTAACTTGCCGTTCCTTCCGACTCCCATGGCGAAAGGCATAGTGCCCGATAGCAGCCCTTTAAATGTGAGCAGCGCCCGGAGCCAGGCTCTTAAGATTGCTGACATCGTGTTAGTATTGGGAGCTCGCCTGAATTGGATCCTTCACTTCGGAACGAGTCCAAAGTGGAATAGCGAGAGTATTTTTATACAATTTGACAGTAACCCGGAAACGCTCGGAGACAATAACGTTAGTCCCGGAGCAGATTTAAGTATCTGGGGGGACATCGGACTATCGGTTACTGCACTGGTAGAAGAGCTCACGAGACAGGACAGTTGTTGGAAATACTCGGGAGTCAAACAGGAGATCAGGGAAAAGATACAGTTAAACCAGACCAGATTGCTCCGTAAGGAAAAAACGCGTGGAGCTCAGCTTAACTATAATCAAGTGTACGGCACCCTTCGTCCGTTAATTGACGACTATCGCACGATCTTGGTCACAGAAGGTGCCAACACAATGGACATCGCTAGAATAAGTTTTCCGACTGATGCACCTCGCAGAAGACTTGATGCTGGTACAAATGCTACAATGGGTATAGGTTTAGGTTACGCATTAGCGTGTAAAGCCAGCCACCCTGAGTTAGACGTTGTTTTAATCCAAGGCGACAGTGCCTTCGGTTTTAGCGCTATGGAGATCGAGACTGCCGTCCGTTGCCAATTAGCCCTTGTGATTGTCGTAATGAATAACTCTGGGATATATCACGGTGAGAAAGACATCGAAGGAGATCTGCCCCCGACCGCTTTGTCAAAAAATTGTAGATATGACTTGGTCGGTAAGGGTTTAGGAGCTAACGACTTCTTCGTCAATACCATCTCAGAGTTATCTCGCTGTTTTCAGCAGGCAGTTCAATTGAGCAGAACCAAGCGCGAAACCTCCGTCATTAATGTCATAATCGAACCGGGAGAGCAGAAACAGATCGCGTTCGCATGGCAAAACAAGCCTAGACTGTAAGGTTCCTAACCTGCCTGGCATCTTAATCAATTTTAAACTCACCAAATCAAAATATGAAAAAAAAAAATAATAAAATGTCAACTCCACTTCAAGGAATTAAAGTTCTCGATTTCACCGGTGTGCAATCTGGCCCATCTTGTACTCAAATGCTGGCCTGGTTTGGCGCTGACGTTATTAAAATTGAACGTCCCGGCGTTGGTGACGTAACGCGTCACCAGCTGCGAGATATTCCTGATATCGATGCGCTTTACTTCACCATGCTTAACAGTAACAAACGTTCTATTGAGTTAAATACCAAAACAGCGGAAGGCAAAGAGGTAATGGAAAAGCTGATCCGCGAAGCTGATATCTTAGTCGAGAACTTTCATCCAGGGGCCATTGATCACATGGGCTTCACCTGGGAGCATATTCAAGAAATCAATCCACGTCTGATTTTTGGTTCGATCAAAGGGTTTGATGAGTGTTCGCCTTATGTGAATGTAAAAGCCTATGAAAACGTTGCTCAGGCAGCGGGTGGCGCGGCATCCACTACGGGTTTTTGGGATGGTCCGCCGCTGGTAAGCGCTGCAGCGTTGGGTGACAGCAACACCGGAATGCATTTGCTGATCGGTTTACTTGCTGCTTTGCTGCATCGCGAAAAAACGGGGCGTGGGCAACGAGTCACCATGTCAATGCAGGATGCCGTATTGAACCTTTGCCGCGTGAAATTACGTGACCAGCAGCGTCTAGATAAATTGGGTTATCTGGAAGAATACCCGCAGTATCCGAATGGTACATTTGGTGATGCAGTTCCCCGCGGTGGTAATGCAGGTGGTGGCGGTCAGCCTGGCTGGATCCTGAAATGTAAAGGCTGGGAAACCGATCCTAACGCCTATATTTATTTCACTATTCAGGAGCAAAACTGGGAAAACACCTGTAAAGCCATCGGCAAACCAGAATGGATTACCGATCCGGCATACAGTACAGCCCATGCACGACAGCCACATATTTTCGATATTTTTGCTGAAATCGAAAAATACACTGTCACTATTGATAAACATGAAGCGGTGGCCTATTTGACTCAGTTTGATATTCCTTGTGCACCGGTTTTAAGTATGAAAGAAATTTCACTTGATCCCTCTTTGCGCCAAAGTGGCAGTGTTGTTGAAGTGGAACAACCGTTGCGTGGAAAATATCTGACCGTTGGTTGTCCAATGAAATTCTCTGCCTTTACGCCGGATATTAAAGCTGCGCCGCTATTAGGTGAACATACCGCTGCTGTATTGCAGGAGCTGGGTTATAGCGACGATGAAATTGCTGCAATGAAGCAAAACCACGCCATCTGAGGTTCCTAAACGACCTGGCATCATCTACCTTTATTAATCAACAATCCAAATTATTAATAAAAAAAATTTAAAAAATGACAAATAATGAAAGCAAAGGGCCGTTTGAAGGCTTATTAGTTATCGATATGACACATGTCCTTAATGGACCTTTCGGAACTCAACTTCTTTGTAATATGGGCGCAAGGGTAATTAAAGTTGAGCCGCCGGGTCATGGTGATGATACCCGCACATTTGGTCCCTATGTGGATGGACAGTCACTCTATTACAGTTTTATTAATCATGGCAAAGAGAGTGTGGTTCTTGATTTAAAGAATGATCACGATAAAAGTATATTTATAAATATGCTCAAACAAGCTGATGTATTAGCTGAGAATTTTCGCCCAGGTACAATGGAAAAACTGGGGTTTTCATGGGAAACGCTTCAAGAAATCAACCCGCGCCTCATATATGCTTCATCGTCAGGTTTCGGACATACCGGTCCGCTAAAAGATGCTCCTGCCTACGATACCATCATTCAGGCAATGAGCGGGATAATGATGGAAACAGGATATCCTGATGCTCCGCCAGTGCGCGTTGGTACATCTCTTGCGGATCTATGCGGCGGTGTCTATTTATTCAGCGGAATAGTGAGTGCACTTTATGGCCGCGAAAAGAGCCAGAGAGGGGCGCATGTCGATATAGCGATGTTTGATGCCACGCTGAGTTTTCTGGAGCATGGTCTGATGGCATATATCGCAACTGGGAAGTCACCACAACGTCTGGGAAATCGCCATCCCTACATGGCACCTTTTGATGTTTTCAATACTCAGGATAAGCCGATTACGATTTGTTGTGGTAATGACAAGCTTTTTTCTGCGTTATGCCAGGCACTGGAGCTTACGGAACTGGTTAATGATCCCCGATTTAGCAGCAATATTTTACGCGTACAAAACCAGGCTATTCTTAAACAATATATTGAGCGGACGTTAAAAACGCAGGCAGCTGAAGTTTGGTTAGCCAGAATACATGAAGTTGGTGTACCCGTCGCGCCGTTATTAAGTGTGGCTGAGGCCATTAAATTGCCACAAACTCAGGCGAGAAATATGTTGATTGAAGCCGGGGGAATAATGATGCCGGGTAATCCGATAAAAATCAGCGGCTGCGCGGACCCGCATGTTATGCCGGGAGCGGCAACGCTCGACCAGCATGGGGAACAAATTCGCCAGGAGTTCTCATCATAAGGTTCCTAAGGGTCCTGGCATCACCTAAAAATTATAAATAAATTAATAAACCTTTTAACATAAAAATTAATAAAATGAATAATCCACAAACAGGACAATCAACAGGCCTCTTGGGCAATCGTTGGTTCTACTTGGTATTAGCAGTTTTGCTGATGTGTATGATCTCGGGTGTCCAATATTCCTGGACACTGTACGCTAACCCGGTTAAAGACAACCTTGGCGTTTCTTTGGCTGCGGTTCAGACGGCTTTCACACTCTCTCAGGTCATTCAAGCTGGTTCTCAGCCTGGTGGTGGTTACTTCGTTGATAAATTCGGTCCAAGAATTCCATTGATGTTCGGTGGTGCGATGGTTCTCGCTGGCTGGACCTTCATGGGTATGGTTGACAGTGTTCCTGCTCTGTATGCTCTTTATACTCTGGCCGGTGCAGGTGTTGGTATCGTTTACGGTATCGCGATGAACACGGCTAACAGATGGTTCCCGGACAAACGCGGTCTGGCTTCCGGTTTCACCGCTGCCGGTTACGGTCTGGGTGTTCTGCCGTTCCTGCCACTGATCAGCTCCGTTCTGAAAGTTGAAGGTGTTGGCGCAGCATTCATGTACACCGGTTTGATCATGGGTATCCTGATTATCCTGATCGCTTTCGTTATCCGTTTCCCTGGCCAGCAAGGCGCCAAAAAACAAATCGTTGTTACCGACAAGGATTTCAATTCTGGCGAAATGCTGAGAACACCACAATTCTGGGTTCTGTGGACCGCATTCTTTTCCGTTAACTTTGGTGGTTTGCTGCTGGTTGCCAACAGCGTCCCTTACGGTCGCAGCCTCGGTCTTGCCGCAGGTGTGCTGACGATCGGTGTTTCGATCCAGAACCTGTTCAATGGTGGTTGCCGTCCTTTCTGGGGTTTCGTTTCCGATAAAATCGGCCGTTACAAAACCATGTCCGTCGTTTTCGGTATCAATGCTGTTGTTCTCGCACTTTTCCCGACGATTGCTGCCTTGGGCGATGTAGCCTTTATCGCCATGTTGGCAATCGCATTCTTCACATGGGGTGGTAGCTACGCTCTGTTCCCATCGACCAACAGCGATATTTTCGGTACGGCATACTCTGCCAGAAACTATGGTTTCTTCTGGGCTGCAAAAGCAACTGCCTCGATCTTCGGTGGTGGTCTGGGTGCTGCAATTGCAACCAACTTCGGATGGAATACCGCTTTCCTGATTACTGCGATTACTTCTTTCATCGCATTTGCTCTGGCTACCTTCGTTATTCCAAGAATGGGCCGTCCAGTCAAGAAAATGGTCAAATTGTCTCCAGAAGAAAAAGCTGTACATTAAGGTTCCTAATTCCCCTGGCATCCCCAAAAAATTAATTCATTTTCTCTTTTCAACAATTTAAAAAAAATTAAAAAATGAGTAACGACGACAATGTAGAGTTGACTGATGGCTTTCATGTTTTGATCGATGCCCTGAAAATGAATGACATCGATACCATGTATGGTGTTGTCGGCATTCCTATCACGAACCTGGCTCGTATGTGGCAAGATGACGGTCAGCGTTTTTACAGCTTCCGTCACGAACAACACGCAGGTTATGCAGCTTCTATCGCCGGTTACATCGAAGGAAAACCTGGCGTTTGCTTGACCGTTTCCGCCCCTGGCTTCCTGAACGGCGTGACTTCCCTGGCTCATGCAACCACCAACTGCTTCCCAATGATCCTGTTGAGCGGTTCCAGTGAACGTGAAATCGTCGATTTGCAACAGGGCGATTACGAAGAAATGGATCAGATGAATGTTGCACGTCCACACTGCAAAGCTTCTTTCCGTATCAACAGCATCAAAGACATTCCAATCGGTATCGCTCGTGCAGTTCGCACCGCTGTATCCGGACGTCCAGGTGGTGTTTACGTTGACTTGCCAGCAAAACTGTTCGGTCAGACCATTTCTGTAGAAGAAGCTAACAAACTGCTCTTCAAACCAATCGATCCAGCTCCGGCACAGATTCCTGCTGAAGATGCTATCGCTCGCGCTGCTGACCTGATCAAGAACGCCAAACGTCCAGTTATCATGCTGGGTAAAGGCGCTGCATACGCACAATGCGACGACGAAATCCGCGCACTGGTTGAAGAAACCGGCATCCCATTCCTGCCAATGGGTATGGCTAAAGGCCTGCTGCCTGACAACCATCCACAATCCGCTGCTGCAACCCGTGCTTTCGCACTGGCACAGTGTGACGTTTGCGTACTGATCGGCGCTCGTCTGAACTGGCTGATGCAGCACGGTAAAGGCAAAACCTGGGGCGACGAACTGAAGAAATACGTTCAGATCGACATCCAGGCTAACGAAATGGACAGCAACCAGCCTATCGCTGCACCAGTTGTTGGTGACATCAAGTCCGCCGTTTCCCTGCTCCGCAAAGCACTGAAAGGCGCTCCAAAAGCTGACGCTGAATGGACCGGCGCTCTGAAAGCCAAAGTTGACGGCAACAAAGCCAAACTGGCTGGCAAGATGACTGCCGAAACCCCATCCGGAATGATGAACTACTCCAATTCCCTGGGCGTTGTTCGTGACTTCATGCTGGCAAATCCGGATATTTCCCTGGTTAACGAAGGCGCTAATGCACTCGACAACACTCGTATGATTGTTGACATGCTGAAACCACGCAAACGTCTTGACTCCGGTACCTGGGGTGTTATGGGTATTGGTATGGGCTACTGCGTTGCTGCAGCTGCTGTTACCGGCAAACCGGTTATCGCTGTTGAAGGCGATAGCGCATTCGGTTTCTCCGGTATGGAACTGGAAACCATCTGCCGTTACAACCTGCCAGTTACCGTTATCATCATGAACAATGGTGGTATCTATAAAGGTAACGAAGCAGATCCACAACCAGGCGTTATCTCCTGTACCCGTCTGACCCGTGGTCGTTACGACATGATGATGGAAGCATTTGGCGGTAAAGGTTATGTTGCCAATACTCCAGCAGAACTGAAAGCTGCTCTGGAAGAAGCTGTTGCTTCCGGCAAACCATGCCTGATCAACGCGATGATCGATCCAGACGCTGGTGTCGAATCTGGCCGTATCAAGAGCCTGAACGTTGTAAGTAAAGTTGGCAAGAAATAAGGTTCCTAAAATGCCTGGCATCTAAACTAAAATACAATAACCAAAAATCATCTATGCGTAAAAAAAATTATAAAATGGATAGCGATACTCTCTCAGGATTATTGGAAAACGTCGCCAAAAAATTCCCCGATCGCCGAGCTCTCTCCGTTTCTGGAAAATTCAATCTCACTCACGCGCGTCTTCACGATCTAATCGAACGCGCCGCTTCACGCCTTGTCTCCGACGCTGGAATCAAACCCGGCGATGTCGTTGCTCTCACCTTCCCTAACACCGTCGAGTTTGTTATAATGTTTTTAGCGGTGATAAGAGCTAGAGCCACGGCGGCGCCGTTGAACGCAGCGTACACGGCGGAGGAATTTGAGTTTTACCTCTCCGATTCAGATTCAAAGCTATTGTTAACCTCTAAAGAAGGAAACGCACCGGCTCAAGAAGCAGCTTCAAAGCTGAAAATCTCTCACGTCACCGCTACGCTGCTTGACGCTGGCTCGGACCTTGTACTATCCGTTGCGGATTCAGATTCCGTCGTTGACTCAGCGACGGAACTCGTTAATCACCCGGACGACGGTGCTCTCTTCCTCCACACTTCTGGCACTACGAGCCGTCCAAAGGGTGTACCGCTTACGCAGCTCAATCTAGCTTCATCCGTCAAGAACATTAAAGCTGTGTACAAGCTTACTGAGTCTGATTCTACGGTGATTGTTCTCCCTCTGTTCCATGTTCATGGATTGTTAGCTGGGTTGCTTAGCTCGCTTGGAGCTGGTGCTGCTGTAACTCTTCCAGCTGCTGGTAGATTCTCAGCAACAACATTTTGGCCAGATATGAAGAAGTATAACGCTACATGGTATACTGCTGTGCCGACCATTCATCAGATCATATTGGACCGCCACGCGAGCCACCCTGAAACGGAATATCCTAAACTCCGGTTCATCAGGAGTTGCAGTGCTTCTTTGGCTCCGGTGATATTGTCCAGGCTTGAGGAAGCGTTTGGAGCACCGGTGCTCGAGGCCTATGCAATGACAGAGGCAACACATTTGATGAGCTCAAACCCCTTACCAGAGGAAGGTCCACACAAGCCTGGGTCTGTTGGGAAACCGGTAGGTCAAGAAATGGCGATCCTTAATGAGAAAGGCGAGATCCAAGAGCCAAATAACAAAGGAGAGGTTTGTATAAGAGGTCCAAATGTGACCAAGGGTTACAAGAATAACCCAGAAGCCAACAAGGCAGGTTTCGAGTTTGGGTGGTTCCACACTGGTGATATCGGTTACTTTGATACCGATGGGTATTTGCATCTGGTGGGTCGGATCAAAGAGCTTATTAACCGTGGAGGTGAGAAGATATCTCCAATTGAAGTGGATGCAGTACTCTTAACGCATCCTGACGTTTCTCAGGGTGTTGCATTCGGTGTTCCTGATGAGAAATATGGGGAAGAGATTAACTGTGCGGTGATTCCAAGAGAAGGAACTACTGTAACCGAAGAGGACATTAAAGCGTTTTGTAAGAAGAATTTGGCAGCTTTCAAGGTGCCAAAGAGAGTGTTCATCACTGATAACCTCCCCAAAACTGCCTCTGGTAAGATTCAGCGCCGTATCGTCGCACAACATTTCCTTGAGAAGCCCTGAGGTTCCTAAAAACCCTGGCATCTTTTCCATAACAAAAATTAAACCTCCTCCATATGAAAAATAAAAATTATAAAATGACAAGTGCCGCTACTGTTACTGCTTCGTTCAACGATACTTTTAGCGTATCCGATAATGTCGCCGTTATTGTTCCTGAAACGGACACTCAGGTGACCTACAGGGATCTATCCCACATGGTGGGTCACTTCCAGACCATGTTCACAAATCCTAATTCTCCATTGTACGGAGCTGTTTTCAGACAAGATACAGTGGCGATATCCATGCGTAATGGGCTGGAATTTATCGTCGCTTTCCTCGGTGCTACTATGGACGCTAAAATTGGCGCGCCCTTGAATCCCAATTATAAGGAAAAGGAGTTCAATTTTTATTTGAATGACCTGAAATCTAAGGCGATTTGCGTCCCAAAGGGTACCACAAAGTTACAGAGTTCTGAAATTCTAAAATCTGCCTCCACGTTTGGATGTTTTATCGTAGAGCTGGCCTTCGATGCGACCAGGTTTAGGGTAGAGTATGATATATACTCTCCAGAGGACAACTACAAAAGGGTTATTTACCGGTCTTTGAACAACGCCAAATTTGTCAACACAAATCCCGTTAAATTCCCTGGGTTTGCCCGTTCCAGTGACGTTGCCCTGATTTTGCATACCAGTGGTACCACCTCCACTCCAAAAACGGTGCCTTTGTTACATTTGAACATTGTGAGAAGCACGTTGAACATTGCTAACACTTACAAGCTAACGCCCTTGGACAGATCTTATGTCGTGATGCCTCTTTTCCACGTCCATGGGTTAATTGGTGTTTTACTTTCCACTTTTAGAACTCAGGGTTCTGTTGTGGTTCCCGATGGATTCCATCCAAAGTTATTCTGGGACCAATTTGTTAAGTACAACTGTAATTGGTTCAGTTGCGTTCCCACAATAAGCATGATTATGCTGAACATGCCCAAACCAAACCCTTTCCCACACATTAGATTCATCAGATCGTGTTCTTCTGCTTTGGCTCCAGCAACGTTCCATAAGCTGGAGAAGGAATTCAATGCACCTGTCTTGGAGGCCTATGCGATGACCGAAGCATCACATCAAATGACCTCAAACAATCTGCCTCCAGGAAAGAGAAAGCCTGGTACTGTGGGCCAGCCACAAGGAGTCACCGTCGTCATTCTAGATGACAATGACAATGTCTTGCCCCCGGGCAAAGTCGGCGAAGTTTCCATCAGAGGCGAAAACGTCACTTTGGGGTATGCTAATAATCCAAAAGCTAACAAGGAGAACTTCACCAAGAGAGAGAACTATTTCAGAACCGGTGACCAAGGTTATTTCGACCCTGAGGGGTTTTTGGTCCTTACAGGCAGAATCAAAGAGCTTATCAACAGGGGTGGTGAAAAGATTTCACCCATTGAGCTCGACGGCATTATGCTATCGCATCCAAAGATCGATGAAGCCGTTGCATTTGGTGTTCCCGACGATATGTACGGCCAAGTAGTTCAAGCCGCCATTGTTTTGAAGAAGGGAGAAAAAATGACCTACGAAGAACTGGTGAACTTCTTAAAGAAGCACCTAGCCTCTTTCAAAATTCCAACCAAGGTGTACTTTGTTGATAAGCTACCAAAAACCGCTACAGGTAAAATCCAGAGAAGAGTTATCGCAGAAACTTTTGCTAAGAGCAGCAGAAATAAGAGTAAGTTGTAGGGTTCCTAGCTGATTAGAAGGCCATCCTGACGGATGGCCTTTTTTTTGACTCTGTTGTGTCTTCTTTTAGGTGTGGAATAATTTTCCAACCATCACTTTATCTGTCTCCCGAACAGCACCTTATTCAGCCATCGGTTCACATACACGTTCACGAAAGCACATCCTACTCCGATAGCGGCTCCTGCCACTACATCACTGGGATAGTGCACCCCTTGGTTCATCCGGGCAAACCCTACTCCGCAAGCCCATACGGCTGAAGGGGCGATAACATACCACTTAGGATAAGTGATGGAGAGTGAGGTGGCTAAAGAAAAAGCTGCCGCAGTGTGTCCTGAAGGGAATGACGGACTATCGGCATCCGGTGCTCCGTATGCATGAATCTTATCCGGATACTTTACAAAAGGCCGGTCACGACCTATTATATGTTTGGCGGCGTATGTAATCCCCACTGCTTCAATCACACTGGTTCCTATGTAAATGGCATCTTTCAGCATCGGCTGGTCTTTCTTTATCAATGCATAGAGTCCCATGGCTGTAGGTATTCCTACGGGTAGAATCACTCCGCTAGCGGACAATCCGCGGCTCAGTCCGTGTACATCCCATCGGTTGATGGTTTTCAACGTGTTGATGTCTATATTCTGTGCGCTGAGGCTGAATGTAAAACAGGCTGTCAGTATAAGAAAAATAATCCTTTGGCGTTTCATTTAGTTTGGCATTTGTTCAGTGCAAAGATAGGAAAATAATGTAATTCGCATTTACCCTTCCCCGAATTAAAGAATAATTTCCATAATTCCTTGTATTATCGCTAGAATTCCTTTACTTTTGCATCGTGAATACACAGCTTTTAACAGAATTTTTAGTTCTAACAATTAAATAATTTAAATTCAATCATTTATGTGGTTAAGTAATTCATCTATTGGAAGGAAAGTGGTGATGAGTGTCACAGGTATCGCCCTTGTCCTGTTTCTGACGTTTCACATGGCGATGAACCTTGTTGCGCTCTTCTCGGAAGATGCTTACAACATGATTTGTGAATTCTTGGGAGCTAACTGGTACGCGTTGGTTGCAACTGCCGGGCTGGCTGCTCTGTTTGTAATTCACATCATCTACGCTTTCTGGTTGACCATGCAGAACCGTGCAGCTCGCGGTAGCGAACGTTATGCAGTGAACGTTAAACCTAAAACTGTGGAATGGGCATCTCAGAACATGCTTGTCCTGGGTATTGTCGTTATTGCCGGATTGGCTCTTCATTTTGCCAATTTCTGGTACAAAATGCAGTTTGCCGAAATTATCGGTGTTCATGATTTGGGCGCATTCGGTCCTACAGACGGTGCCGCTTACATTCGTGAAACATTCGGATGCCCTGTATTTACAGTGCTTTACCTCATTTGGTTAGCAGCTTTGTGGTTCCACCTGACTCACGGATTCTGGAGTGCTTTGCAGACTTTGGGCTGGAGCAATAAAATCTGGTTCGAGCGTTGGAAGACTATTGGTAACATCTACTCTACTTTGGTAGTTCTTGGTTTTGCAGCAGTAGTCGTTATCTACTTCGCTATGACTGGCGCGCCTGATAGGTGGGCTGCCCTTCCTGGTTGGCTTGGTTTCATCAGCCATCCGCTTGCCCTCATCTGTTACGCCGGCGGTAGCCGGCCAGCCTCGCAGAGCAGGATTCCCGTTGAGCACCGCCAGGTGCGAATAAGGGACAGTGAAGAAGGAACACCCGCTCGCGGGTGGGCCTACTTCACCTATCCTGCCCGGCTGACGCCGTTGGATACACCAAGGAAAGTCTACACGAACCCTTTGGCAAAATCCTGTATATCGTGCGAAAAAGGATGGATATACCGAAAAAATCGCTATAATGACCCCGAAGCAGGGTTATGCAGCGGAAAAGCGGGATTAAAAGTCGGGGATTGGTGAACAAAAAGGTGTTTCTCTCTTTAAGAGAAATATCGTTTTGCTAAACAGTTGATATTGAGGTATCATTTTATCGTAAAAGACATTTTTGCTCAACAATTGCTTGACGGAAATCAACAAATTTTAGCATTTTGTAAAAAAGTCGCTATATAATTTGGTGAATTGGAGTTATTTTCATATTTTTGCATCCCGAAGAGTTTCTCTTAAAGAGAGAAACATCTTTTGCATACCTTTTCCGACCGAATTTTTATGTCGTAAAGAGGGGCTTTGCAGGGGGTGGACTCAGAAAGATGAGAATAGATGACTATTGTAGTTGAAACACATAGAAAGTTGCTGATATACAGACCGATACGCATATCGGGATGAACCATGAGTACGTTCTTTTCTCAAAAAACATAAATATTCGAAAAGAGATGCAATAAATTAAGGAGAGGTTATAATGAACAAAGTAAATATAAAAGATAGTCAAAATTTTATTACTTCAAAATATCACATAGAAAAAATAATGAATTGCATAAGTTTAGATGAAAAAGATAACATCTTTGAAATAGGTGCAGGGAAAGGTCATTTTACTGCTGGATTGGTAAAGAGATGTAATTTTGTAACGGCGATAGAAATTGATTCTAAATTATGTGAGGTAACTCGTAATAAGCTCTTAAATTATCCTAACTATCAAATAGTAAATGATGATATACTGAAATTTACATTTCCTAGCCACAATCCATATAAAATATTTGGCAGCATACCTTACAACATAAGCACAAATATAATTCGAAAAATTGTTTTTGAAAGTTCAGCCACAATAAGTTATTTAATAGTGGAATATGGTTTTGCTAAAATGTTATTAGATACAAACAGATCACTAGCATTGCTGTTAATGGCAGAGGTAGATATTTCTATATTAGCAAAAATTCCTAGGTATTATTTCCATCCAAAACCTAAAGTGGATAGCACATTAATTGTATTAAAAAGAAAGCCAGCAAAAATGGCATTTAAAGAGAGAAAAAAATATGAAACTTTTGTAATGAAATGGGTTAACAAAGAGTACGAAAAACTGTTTACAAAAAATCAATTTAATAAAGCTTTAAAACATGCGAGAATATATGATATAAACAATATTAGTTTCGAACAATTTGTATCGCTATTTAATAGTTATAAAATATTTAACGGCTAAAAACAATAGGCCACATGCAACTGTAAATGTTTACGCGGGTACCGACACCGCGGTGGAGGGGAATTGTGTTACAACCAATTAACCAATTCTGATTAGAAAAACTCATCGAGCATCAAATGAAACTGCAATTTATTCATATCAGGATTATCAATACCATATTTTTGAAAAAGCCGTTTCTGTAATGAAGGAGAAAACTCACCGAGGCAGTTCCATAGGATGGCAAGATCCTGGTATCGGTCTGCGATTCCGACTCGTCCAACATCAATACAACCTATTAATTTCCCCTCGTCAAAAATAAGGTTATCAAGTGAGAAATCACCATGAGTGACGACTGAATCCGGTGAGAATGGCAAAAGCTTATGCATTTCTTTCCAGACTTGTTCAACAGGCCAGCCATTACGCTCGTCATCAAAATCACTCGCATCAACCAAACCGTTATTCATTCGTGATTGCGCCTGAGCGAGGCGAAATACGCGATCGCTGTTAAAAGGACAATTACAAACAGGAATCGAATGCAACCGGCGCAGGAACACTGCCAGCGCATCAACAATATTTTCACCTGAATCAGGATATTCTTCTAATACCTGGAATGCTGTTTTCCCGGGGATCGCAGTGGTGAGTAACCATGCATCATCAGGAGTACGGATAAAATGCTTGATGGTCGGAAGAGGCATAAATTCCGTCAGCCAGTTTAGTCTGACCATCTCATCTGTAACATCATTGGCAACGCTACCTTTGCCATGTTTCAGAAACAACTCTGGCGCATCGGGCTTCCCATACAATCGATAGATTGTCGCACCTGATTGCCCGACATTATCGCGAGCCCATTTATACCCATATAAATCAGCATCCATGTTGGAATTTAATCGCGGCCTGGAGCAAGACGTTTCCCGTTGAATATGGCTCATAACACCCCTTGTATTACTGTTTATGTAAGCAGACAGTTTTATTGTTCATGATGATATATTTTTATCTTGTGCAATGTAACATCAGAGATTTTGAGACACAACGTGGCTTTGTTGAATAAATCGAACTTTTGCTGAGTTGAAGGATCAGAGCCTACGTTCCGAATACGGTCAAAAAAAAGGCCATCCGTCAGGATGGCCTTCGCATTAATATGCCGCTTCGAATTCTTTTAGGAAGCGTGTATCGTTTTCAGAGAACATACGGAGGTCTTTCACCTGATATTTCAGGTTTGTGATACGCTCGATACCCATACCGAGTCCATAACCGCTGTATATTTTGCTGTCTATACCATTTGATTCAAGTACGTTCGGGTCTACCATACCGCAACCGAGGATTTCTACCCAGCCGGTGTGTTTACAGAACGGACATCCTTTACCGCCGCAGATATTACAGCTGATATCCATTTCCGCACTTGGTTCAGCAAACGGGAAGTAAGACGGACGCAGACGGATCTTTGTATCAGCACCGAACATTTCTTTGGCAAAGAGCAGCAATACCTGCTTCAAGTCGGTGAATGATACGTTTTTATCTACATACAGCGCTTCTACCTGATGGAAGAAACAGTGTGCGCGATAGCTGATAGCTTCGTTACGATATACACGTCCCGGACAGATGATGCGGATAGGAGGCTGTGAAGTTTCCATCACACGAGTCTGTACAGAAGAAGTATGTGTACGCAATACTACGTCCGGGTGAGCTTCGATAAAGAAAGTGTCCTGCATATCGCGTGCCGGATGATCTTCGGCAAAGTTCAGTGCCGAGAACACGTGCCAGTCATCTTCAATTTCCGGACCTTCGGCAATGCTGAATCCCAGACGGGCAAAGATATCAATGATTTCGTTCTTTACAATGGTGAGCGGGTGGCGTGTACCGAGTTCTACAGGATAAGCCGAACGCGTCAAATCCAGTCCGTCACAATCGTTGTCCTGACTTTCAAACATTTCTTTCAGCGCGTTGATTTTGTCCTGCGCTTTTGTTTTCAGTTCATTCAGTCTCATGCCGACTTCTTTTTTCTGTTCGGCAGCTACATTACGGAAATCTGCCATTAAGTCGTTAATGGCTCCCTTCTTACTTAGGTATTTGATGCGGAGAGCTTCGAGTTCTTCGGCATTGGAGGCGTGTAAGGCTTCCACCTCTTTCAGAAGTTGTTCAATCTTAGCTATCATTTTTTAATATTTTTAGCGGCCCCGTTAAACAAAATTATTTGTAGAGGCTGTTTCGTCCTCACGGACTCATCAGACCGGAAAGCACATCCGGTGACAGCTCAGGCTACTTTGTTTCTTTCGACACTGCAAATATAAGAACATTATTTGAAAGTTCAAGTGAAACTTTAAATTTTAACAATAGATTAACCATTGCAAACAAAACAAAAAAAAGGTAGCCCAATTGTAAAACGAAAGGCCCAGTCTTTCGACTGAGCCTTTCGTTTTATCCTACAGTCGCTCGGCGATCGAAGGCTTCGGAAAAAAAAGGCCATCCGTCAGGATGGCCTTCGCATTAATATGCCGCTTCGAATTCTTTTAGGAAGCGTGTATCGTTTTCAGAGAACATACGGAGGTCTTTCACCTGATATTTCAGGTTTGTGATACGCTCGATACCCATACCGAGTCCATAACCGCTGTATATTTTGCTGTCTATACCATTTGATTCAAGTACGTTCGGGTCTACCATACCGCAACCGAGGATTTCTACCCAGCCGGTGTGTTTACAGAACGGACATCCTTTACCGCCGCAGATATTACAGCTGATATCCATTTCCGCACTTGGTTCAGCAAACGGGAAGTAAGACGGACGCAGACGGATCTTTGTATCAGCACCGAACATTTCTTTGGCAAAGAGCAGCAATACCTGCTTCAAGTCGGTGAATGATACGTTTTTATCTACATACAGCGCTTCTACCTGATGGAAGAAACAGTGTGCGCGATAGCTGATAGCTTCGTTACGATATACACGTCCCGGACAGATGATGCGGATAGGAGGCTGTGAAGTTTCCATCACACGAGTCTGTACAGAAGAAGTATGTGTACGCAATACTACGTCCGGGTGAGCTTCGATAAAGAAAGTGTCCTGCATATCGCGTGCCGGATGATCTTCGGCAAAGTTCAGTGCCGAGAACACGTGCCAGTCATCTTCAATTTCCGGACCTTCGGCAATGCTGAATCCCAGACGGGCAAAGATATCAATGATTTCGTTCTTTACAATGGTGAGCGGGTGGCGTGTACCGAGTTCTACAGGATAAGCCGAACGCGTCAAATCCAGTCCGTCACAATCGTTGTCCTGACTTTCAAACATTTCTTTCAGCGCGTTGATTTTGTCCTGCGCTTTTGTTTTCAGTTCATTCAGTCTCATGCCGACTTCTTTTTTCTGTTCGGCAGCTACATTACGGAAATCTGCCATTAAGTCGTTAATGGCTCCCTTCTTACTTAGGTATTTGATGCGGAGAGCTTCGAGTTCTTCGGCATTGGAGGCGTGTAAGGCTTCCACCTCTTTCAGAAGTTGTTCAATCTTAGCTATCATTTTTTAATATTTTTAGCGGCCCCGTTAAACAAAATTATTTGTAGAGGCTGTTTCGTCCTCACGGACTCATCAGACCGGAAAGCACATCCGGTGACAGCTCAGGCTACTTTGTTTCTTTCGACACTGCAAATATAAGAACATTATTTGAAAGTTCAAGTGAAACTTTAAATTTTAACAATAGATTAACCATTGCAAACAAAACAAAAAAAAGGTAGCCCAATTGTAAAACGAAAGGCCCAGTCTTTCGACTGAGCCTTTCGTTTTATCCTAGGATCAGCTGTACGTACTCGCAGTTCAACCTGTTGATAGTACGTACTAAGCTCTCATGTTTCACGTACTAAGCTCTCATGTTTAACGTACTAAGCTCTCATGTTTAACGAACTAAACCCTCATGGCTAACGTACTAAGCTCTCATGGCTAACGTACTAAGCTCTCATGTTTCACGTACTAAGCTCTCATGTTTGAACAATAAAATTAATATAAATCAGCAACTTAAATAGCCTCTAAGGTTTTAAGTTTTATAAGAAAAAAAAGAATATATAAGGCTTTTAAAGCTTTTAAGGTTTAACGGTTGTGGACAACAAGCCAGGGATGTAACGCACTGAGAAGCCCTTAGAGCCTCTCAAAGCAATTTTGAGTGACACAGGAACACTTAACGGCTGACATGGGGCGGCCGCGTAG
SEQ ID NO:85(用于基因组整合进普通拟杆菌基因组中的编码草酸盐降解途径的质粒)：
GGAGAGCTGTATGTGGGGTGGAAGAAGCTATCGTAAAAATGCTGGAGCAGCTTGATCCTCATTCTACCTATTCTGATCCGGAGGAAGTGAAGAGAATGAACGAGCCGTTGCAGGGAAACTTCGAAGGTATCGGTATACAGTTTAATATGGCGGAGGACACTTTGCTGGTGATACAGCCTGTATCGGGCGGACCGTCCGAAAAAGTCGGTATTCTGGCCGGTGATCGTATCGTCATGGTGGAAGATACGCTGATAGCCGGTGTAAAGATGAGTACCGAGGATATTATGCGTCGCCTCCGTGGTCCTAAAGATTCAAAAGTGAATCTGAAAATACTTCGCCGAGGGGTGAAGGAACTGCTCCCCTTTACGGTGAAAAGGGATAAAATACCTGTTTACAGCTTGGATGCTTCCTATATGATTAAAGATAAGATCGGTTATATTCGTATCAACCGTTTTGCCGCCACTACCCATGAGGAGTTCAAGAAAGCGCTGACCGGGTTGCAGAAGAAAGGAATGAAAGACTTGATTCTTGATTTGCAGGGCAATGGCGGGGGGTATTTGAATGCTGCTATCGATATTGCCAATGAATTTTTGGGTAAAAAGGAACTGATTGTCTATACGGAGGGCCGTCGTAATCCTCGTAGCGAGTTTTTTGCCAAAGGTACGGGCGAGTTTCAGAACGGTCGTCTGATGGTATTGGTTGATGAATTTTCGGCTTCTGCCAGTGAGATTGTGACCGGAGCTGTTCAGGATTGGGACAGAGGCGTTGTGGTAGGCCGTCGCAGTTTTGGTAAAGGCTTGGTGCAGCGTCCCATTGATTTGCCGGACGGTTCGATGATCCGTCTGACGGTGGCCCGCTATTATACGCCTGCCGGACGTTGTATCCAGAAACCTTACGAGAGCATTGAAAAGTATAATGAAGACCTGATAGACCGTTATAATAAAGGAGAAATGATGAGTGAAGACAGCATCCACTTTCCGGATTCACTGAAGTTCAAGACTCATAGGTTGGCCCGTACGGTATATGGTGGCGGAGGGATCATGCCCGATTATTTTGTGCCGATTGATACGACTCTCTATACTAAATACCATCGTCAGTTGCGTGATAAAGGAGCATTGATGAAAGCTCATTTCCATTTTATTGATGCCCATCGTAAAGAATGGCTGGGCAAATACAAGACTTTCAATGAGTTCTACAAACGTTTTGAAGTGACTCCGGATATGTTGACACAACTGGTTGCTACCGGCAAGGAGATGGGAGTGGAATATAATGAAGAAGAGTATCAAAAGGCCCTGCCTCTGCTGAGGTTGCAGATGAAAGCATTGATAGCCCGTGATTTATGGGATATGAATGAATATTATCACGTCATTAATGACGCGAATGAAAGTATCAGGAAAGCGCTGGAACTGTTGGAGCAACCGGACTTTGAGGGGCTGCTGTTGAAAAAGCGATAGTCAAGACTTTATATTAACTGCAGGGGCGCATAGTTGCACTCCTGCAGTTGTCTGAAAAACTTGTAGGATAAAACGAAAGGCTCAGTCGAAAGACTGGGCCTTTCGTTTTACAATTGGGCTACCTTTTTTTTGTTTTGTTTGCAATGGTTAATCTATTGTTAAAATTTAAAGTTTCACTTGAACTTTCAAATAATGTTCTTATATTTGCAGTGTCGAAAGAAACAAAGTAGCCTGGCATCACCTAAAAATTATAAATAAATTAATAAACCACAATACTAAAAATTTAATAAAATGAATAATCCACAAACAGGACAATCAACAGGCCTCTTGGGCAATCGTTGGTTCTACTTGGTATTAGCAGTTTTGCTGATGTGTATGATCTCGGGTGTCCAATATTCCTGGACACTGTACGCTAACCCGGTTAAAGACAACCTTGGCGTTTCTTTGGCTGCGGTTCAGACGGCTTTCACACTCTCTCAGGTCATTCAAGCTGGTTCTCAGCCTGGTGGTGGTTACTTCGTTGATAAATTCGGTCCAAGAATTCCATTGATGTTCGGTGGTGCGATGGTTCTCGCTGGCTGGACCTTCATGGGTATGGTTGACAGTGTTCCTGCTCTGTATGCTCTTTATACTCTGGCCGGTGCAGGTGTTGGTATCGTTTACGGTATCGCGATGAACACGGCTAACAGATGGTTCCCGGACAAACGCGGTCTGGCTTCCGGTTTCACCGCTGCCGGTTACGGTCTGGGTGTTCTGCCGTTCCTGCCACTGATCAGCTCCGTTCTGAAAGTTGAAGGTGTTGGCGCAGCATTCATGTACACCGGTTTGATCATGGGTATCCTGATTATCCTGATCGCTTTCGTTATCCGTTTCCCTGGCCAGCAAGGCGCCAAAAAACAAATCGTTGTTACCGACAAGGATTTCAATTCTGGCGAAATGCTGAGAACACCACAATTCTGGGTTCTGTGGACCGCATTCTTTTCCGTTAACTTTGGTGGTTTGCTGCTGGTTGCCAACAGCGTCCCTTACGGTCGCAGCCTCGGTCTTGCCGCAGGTGTGCTGACGATCGGTGTTTCGATCCAGAACCTGTTCAATGGTGGTTGCCGTCCTTTCTGGGGTTTCGTTTCCGATAAAATCGGCCGTTACAAAACCATGTCCGTCGTTTTCGGTATCAATGCTGTTGTTCTCGCACTTTTCCCGACGATTGCTGCCTTGGGCGATGTAGCCTTTATCGCCATGTTGGCAATCGCATTCTTCACATGGGGTGGTAGCTACGCTCTGTTCCCATCGACCAACAGCGATATTTTCGGTACGGCATACTCTGCCAGAAACTATGGTTTCTTCTGGGCTGCAAAAGCAACTGCCTCGATCTTCGGTGGTGGTCTGGGTGCTGCAATTGCAACCAACTTCGGATGGAATACCGCTTTCCTGATTACTGCGATTACTTCTTTCATCGCATTTGCTCTGGCTACCTTCGTTATTCCAAGAATGGGCCGTCCAGTCAAGAAAATGGTCAAATTGTCTCCAGAAGAAAAAGCTGTACATTAAGGTTCCTAACCTGCCTGGCATCATCTACCTATATTAATCATCAACCCAATTTTCATATGTATAAAATTAACAAAATGACAAGTGCCGCTACTGTTACTGCTTCGTTCAACGATACTTTTAGCGTATCCGATAATGTCGCCGTTATTGTTCCTGAAACGGACACTCAGGTGACCTACAGGGATCTATCCCACATGGTGGGTCACTTCCAGACCATGTTCACAAATCCTAATTCTCCATTGTACGGAGCTGTTTTCAGACAAGATACAGTGGCGATATCCATGCGTAATGGGCTGGAATTTATCGTCGCTTTCCTCGGTGCTACTATGGACGCTAAAATTGGCGCGCCCTTGAATCCCAATTATAAGGAAAAGGAGTTCAATTTTTATTTGAATGACCTGAAATCTAAGGCGATTTGCGTCCCAAAGGGTACCACAAAGTTACAGAGTTCTGAAATTCTAAAATCTGCCTCCACGTTTGGATGTTTTATCGTAGAGCTGGCCTTCGATGCGACCAGGTTTAGGGTAGAGTATGATATATACTCTCCAGAGGACAACTACAAAAGGGTTATTTACCGGTCTTTGAACAACGCCAAATTTGTCAACACAAATCCCGTTAAATTCCCTGGGTTTGCCCGTTCCAGTGACGTTGCCCTGATTTTGCATACCAGTGGTACCACCTCCACTCCAAAAACGGTGCCTTTGTTACATTTGAACATTGTGAGAAGCACGTTGAACATTGCTAACACTTACAAGCTAACGCCCTTGGACAGATCTTATGTCGTGATGCCTCTTTTCCACGTCCATGGGTTAATTGGTGTTTTACTTTCCACTTTTAGAACTCAGGGTTCTGTTGTGGTTCCCGATGGATTCCATCCAAAGTTATTCTGGGACCAATTTGTTAAGTACAACTGTAATTGGTTCAGTTGCGTTCCCACAATAAGCATGATTATGCTGAACATGCCCAAACCAAACCCTTTCCCACACATTAGATTCATCAGATCGTGTTCTTCTGCTTTGGCTCCAGCAACGTTCCATAAGCTGGAGAAGGAATTCAATGCACCTGTCTTGGAGGCCTATGCGATGACCGAAGCATCACATCAAATGACCTCAAACAATCTGCCTCCAGGAAAGAGAAAGCCTGGTACTGTGGGCCAGCCACAAGGAGTCACCGTCGTCATTCTAGATGACAATGACAATGTCTTGCCCCCGGGCAAAGTCGGCGAAGTTTCCATCAGAGGCGAAAACGTCACTTTGGGGTATGCTAATAATCCAAAAGCTAACAAGGAGAACTTCACCAAGAGAGAGAACTATTTCAGAACCGGTGACCAAGGTTATTTCGACCCTGAGGGGTTTTTGGTCCTTACAGGCAGAATCAAAGAGCTTATCAACAGGGGTGGTGAAAAGATTTCACCCATTGAGCTCGACGGCATTATGCTATCGCATCCAAAGATCGATGAAGCCGTTGCATTTGGTGTTCCCGACGATATGTACGGCCAAGTAGTTCAAGCCGCCATTGTTTTGAAGAAGGGAGAAAAAATGACCTACGAAGAACTGGTGAACTTCTTAAAGAAGCACCTAGCCTCTTTCAAAATTCCAACCAAGGTGTACTTTGTTGATAAGCTACCAAAAACCGCTACAGGTAAAATCCAGAGAAGAGTTATCGCAGAAACTTTTGCTAAGAGCAGCAGAAATAAGAGTAAGTTGTAGGGTTCCTAAACGACCTGGCATCAACTACCTTTATTATTCAACAAACCAATTTAACATATTAAAAAAATAAAAAAATGACTACCACAGCAACGCAGCATTTTGCCCAGTTACTCCAGAAGTACGGAATTGATACTGTCTTCGGCATAGTAGGTATTCCGATCGTGCAGTTGGCCGACACTATGGTCGCGAATGGAATTAAGTTTATACCGTGTCGCAATGAGCAAGCCGCAAGTTACGCGGCAAGCGCCTACGGCTACATCTCGGACAAACCTGGTGTCCTGTTAATCGTGGGAGGGCCTGGCCTTATTCATGCCTTAGCGGGGATCTATAATAGTATGTCGAACCGCTGGCCCTTGTTGGTCATCGCCGGATCCAGCAGCCAGTCAGACATCCACAAGGGAGGTTTCCAGGAACTTGACCAGGTGTCCTTGCTAAGTCCGTTCCTAAAATTCACTGGTAAACTGACGCCGGATAACATCGATATGATAACCCAAAAAGCACTCAACTATTGCATTCAGGGAACCGCCGGAGTGAGCTATATCGATGTCCCCGCTGACTTCATCGAATACGAGAAGCCGCTTGAGGGCAATGATAGAACTGGTAACGAGCTGCCAATGATCCTGACGCCCAATATCTGCGGTCCAGACCCCAGTAAAATTAAGAAGGTGGTGCAATTGATACTCCAGCACAAGAACAAGAACATATTAATAGTGATAGGTAAAGGAGCAGTGAAGAATAGCCATGAGATTAGACGCTTGGTTAACACCTTTAACTTGCCGTTCCTTCCGACTCCCATGGCGAAAGGCATAGTGCCCGATAGCAGCCCTTTAAATGTGAGCAGCGCCCGGAGCCAGGCTCTTAAGATTGCTGACATCGTGTTAGTATTGGGAGCTCGCCTGAATTGGATCCTTCACTTCGGAACGAGTCCAAAGTGGAATAGCGAGAGTATTTTTATACAATTTGACAGTAACCCGGAAACGCTCGGAGACAATAACGTTAGTCCCGGAGCAGATTTAAGTATCTGGGGGGACATCGGACTATCGGTTACTGCACTGGTAGAAGAGCTCACGAGACAGGACAGTTGTTGGAAATACTCGGGAGTCAAACAGGAGATCAGGGAAAAGATACAGTTAAACCAGACCAGATTGCTCCGTAAGGAAAAAACGCGTGGAGCTCAGCTTAACTATAATCAAGTGTACGGCACCCTTCGTCCGTTAATTGACGACTATCGCACGATCTTGGTCACAGAAGGTGCCAACACAATGGACATCGCTAGAATAAGTTTTCCGACTGATGCACCTCGCAGAAGACTTGATGCTGGTACAAATGCTACAATGGGTATAGGTTTAGGTTACGCATTAGCGTGTAAAGCCAGCCACCCTGAGTTAGACGTTGTTTTAATCCAAGGCGACAGTGCCTTCGGTTTTAGCGCTATGGAGATCGAGACTGCCGTCCGTTGCCAATTAGCCCTTGTGATTGTCGTAATGAATAACTCTGGGATATATCACGGTGAGAAAGACATCGAAGGAGATCTGCCCCCGACCGCTTTGTCAAAAAATTGTAGATATGACTTGGTCGGTAAGGGTTTAGGAGCTAACGACTTCTTCGTCAATACCATCTCAGAGTTATCTCGCTGTTTTCAGCAGGCAGTTCAATTGAGCAGAACCAAGCGCGAAACCTCCGTCATTAATGTCATAATCGAACCGGGAGAGCAGAAACAGATCGCGTTCGCATGGCAAAACAAGCCTAGACTGTAAGGTTCCTAAGGGTCCTGGCATCTAAACTAAAATACAATAACCAAAAATCATGTTTAACAAATAAAAATAATAAAATGGATAGCGATACTCTCTCAGGATTATTGGAAAACGTCGCCAAAAAATTCCCCGATCGCCGAGCTCTCTCCGTTTCTGGAAAATTCAATCTCACTCACGCGCGTCTTCACGATCTAATCGAACGCGCCGCTTCACGCCTTGTCTCCGACGCTGGAATCAAACCCGGCGATGTCGTTGCTCTCACCTTCCCTAACACCGTCGAGTTTGTTATAATGTTTTTAGCGGTGATAAGAGCTAGAGCCACGGCGGCGCCGTTGAACGCAGCGTACACGGCGGAGGAATTTGAGTTTTACCTCTCCGATTCAGATTCAAAGCTATTGTTAACCTCTAAAGAAGGAAACGCACCGGCTCAAGAAGCAGCTTCAAAGCTGAAAATCTCTCACGTCACCGCTACGCTGCTTGACGCTGGCTCGGACCTTGTACTATCCGTTGCGGATTCAGATTCCGTCGTTGACTCAGCGACGGAACTCGTTAATCACCCGGACGACGGTGCTCTCTTCCTCCACACTTCTGGCACTACGAGCCGTCCAAAGGGTGTACCGCTTACGCAGCTCAATCTAGCTTCATCCGTCAAGAACATTAAAGCTGTGTACAAGCTTACTGAGTCTGATTCTACGGTGATTGTTCTCCCTCTGTTCCATGTTCATGGATTGTTAGCTGGGTTGCTTAGCTCGCTTGGAGCTGGTGCTGCTGTAACTCTTCCAGCTGCTGGTAGATTCTCAGCAACAACATTTTGGCCAGATATGAAGAAGTATAACGCTACATGGTATACTGCTGTGCCGACCATTCATCAGATCATATTGGACCGCCACGCGAGCCACCCTGAAACGGAATATCCTAAACTCCGGTTCATCAGGAGTTGCAGTGCTTCTTTGGCTCCGGTGATATTGTCCAGGCTTGAGGAAGCGTTTGGAGCACCGGTGCTCGAGGCCTATGCAATGACAGAGGCAACACATTTGATGAGCTCAAACCCCTTACCAGAGGAAGGTCCACACAAGCCTGGGTCTGTTGGGAAACCGGTAGGTCAAGAAATGGCGATCCTTAATGAGAAAGGCGAGATCCAAGAGCCAAATAACAAAGGAGAGGTTTGTATAAGAGGTCCAAATGTGACCAAGGGTTACAAGAATAACCCAGAAGCCAACAAGGCAGGTTTCGAGTTTGGGTGGTTCCACACTGGTGATATCGGTTACTTTGATACCGATGGGTATTTGCATCTGGTGGGTCGGATCAAAGAGCTTATTAACCGTGGAGGTGAGAAGATATCTCCAATTGAAGTGGATGCAGTACTCTTAACGCATCCTGACGTTTCTCAGGGTGTTGCATTCGGTGTTCCTGATGAGAAATATGGGGAAGAGATTAACTGTGCGGTGATTCCAAGAGAAGGAACTACTGTAACCGAAGAGGACATTAAAGCGTTTTGTAAGAAGAATTTGGCAGCTTTCAAGGTGCCAAAGAGAGTGTTCATCACTGATAACCTCCCCAAAACTGCCTCTGGTAAGATTCAGCGCCGTATCGTCGCACAACATTTCCTTGAGAAGCCCTGAGGTTCCTAATTCCCCTGGCATCATCTACCTATATTACTAAACAAACCAATTATAATAATTTTAAAAATAAAAAAATGACAAATAATGAAAGCAAAGGGCCGTTTGAAGGCTTATTAGTTATCGATATGACACATGTCCTTAATGGACCTTTCGGAACTCAACTTCTTTGTAATATGGGCGCAAGGGTAATTAAAGTTGAGCCGCCGGGTCATGGTGATGATACCCGCACATTTGGTCCCTATGTGGATGGACAGTCACTCTATTACAGTTTTATTAATCATGGCAAAGAGAGTGTGGTTCTTGATTTAAAGAATGATCACGATAAAAGTATATTTATAAATATGCTCAAACAAGCTGATGTATTAGCTGAGAATTTTCGCCCAGGTACAATGGAAAAACTGGGGTTTTCATGGGAAACGCTTCAAGAAATCAACCCGCGCCTCATATATGCTTCATCGTCAGGTTTCGGACATACCGGTCCGCTAAAAGATGCTCCTGCCTACGATACCATCATTCAGGCAATGAGCGGGATAATGATGGAAACAGGATATCCTGATGCTCCGCCAGTGCGCGTTGGTACATCTCTTGCGGATCTATGCGGCGGTGTCTATTTATTCAGCGGAATAGTGAGTGCACTTTATGGCCGCGAAAAGAGCCAGAGAGGGGCGCATGTCGATATAGCGATGTTTGATGCCACGCTGAGTTTTCTGGAGCATGGTCTGATGGCATATATCGCAACTGGGAAGTCACCACAACGTCTGGGAAATCGCCATCCCTACATGGCACCTTTTGATGTTTTCAATACTCAGGATAAGCCGATTACGATTTGTTGTGGTAATGACAAGCTTTTTTCTGCGTTATGCCAGGCACTGGAGCTTACGGAACTGGTTAATGATCCCCGATTTAGCAGCAATATTTTACGCGTACAAAACCAGGCTATTCTTAAACAATATATTGAGCGGACGTTAAAAACGCAGGCAGCTGAAGTTTGGTTAGCCAGAATACATGAAGTTGGTGTACCCGTCGCGCCGTTATTAAGTGTGGCTGAGGCCATTAAATTGCCACAAACTCAGGCGAGAAATATGTTGATTGAAGCCGGGGGAATAATGATGCCGGGTAATCCGATAAAAATCAGCGGCTGCGCGGACCCGCATGTTATGCCGGGAGCGGCAACGCTCGACCAGCATGGGGAACAAATTCGCCAGGAGTTCTCATCATAAGGTTCCTAAAATGCCTGGCATCATCTACCTAAATTAATAAACAACCCATTTATAAACCCTTTAAAATTAACAAAATGTCAACTCCACTTCAAGGAATTAAAGTTCTCGATTTCACCGGTGTGCAATCTGGCCCATCTTGTACTCAAATGCTGGCCTGGTTTGGCGCTGACGTTATTAAAATTGAACGTCCCGGCGTTGGTGACGTAACGCGTCACCAGCTGCGAGATATTCCTGATATCGATGCGCTTTACTTCACCATGCTTAACAGTAACAAACGTTCTATTGAGTTAAATACCAAAACAGCGGAAGGCAAAGAGGTAATGGAAAAGCTGATCCGCGAAGCTGATATCTTAGTCGAGAACTTTCATCCAGGGGCCATTGATCACATGGGCTTCACCTGGGAGCATATTCAAGAAATCAATCCACGTCTGATTTTTGGTTCGATCAAAGGGTTTGATGAGTGTTCGCCTTATGTGAATGTAAAAGCCTATGAAAACGTTGCTCAGGCAGCGGGTGGCGCGGCATCCACTACGGGTTTTTGGGATGGTCCGCCGCTGGTAAGCGCTGCAGCGTTGGGTGACAGCAACACCGGAATGCATTTGCTGATCGGTTTACTTGCTGCTTTGCTGCATCGCGAAAAAACGGGGCGTGGGCAACGAGTCACCATGTCAATGCAGGATGCCGTATTGAACCTTTGCCGCGTGAAATTACGTGACCAGCAGCGTCTAGATAAATTGGGTTATCTGGAAGAATACCCGCAGTATCCGAATGGTACATTTGGTGATGCAGTTCCCCGCGGTGGTAATGCAGGTGGTGGCGGTCAGCCTGGCTGGATCCTGAAATGTAAAGGCTGGGAAACCGATCCTAACGCCTATATTTATTTCACTATTCAGGAGCAAAACTGGGAAAACACCTGTAAAGCCATCGGCAAACCAGAATGGATTACCGATCCGGCATACAGTACAGCCCATGCACGACAGCCACATATTTTCGATATTTTTGCTGAAATCGAAAAATACACTGTCACTATTGATAAACATGAAGCGGTGGCCTATTTGACTCAGTTTGATATTCCTTGTGCACCGGTTTTAAGTATGAAAGAAATTTCACTTGATCCCTCTTTGCGCCAAAGTGGCAGTGTTGTTGAAGTGGAACAACCGTTGCGTGGAAAATATCTGACCGTTGGTTGTCCAATGAAATTCTCTGCCTTTACGCCGGATATTAAAGCTGCGCCGCTATTAGGTGAACATACCGCTGCTGTATTGCAGGAGCTGGGTTATAGCGACGATGAAATTGCTGCAATGAAGCAAAACCACGCCATCTGAGGTTCCTAAAAACCCTGGCATCAACTACCTAAATTAATTATCACCCCAAATTAATAACATAAAAATATAATAAAATGAGTAACGACGACAATGTAGAGTTGACTGATGGCTTTCATGTTTTGATCGATGCCCTGAAAATGAATGACATCGATACCATGTATGGTGTTGTCGGCATTCCTATCACGAACCTGGCTCGTATGTGGCAAGATGACGGTCAGCGTTTTTACAGCTTCCGTCACGAACAACACGCAGGTTATGCAGCTTCTATCGCCGGTTACATCGAAGGAAAACCTGGCGTTTGCTTGACCGTTTCCGCCCCTGGCTTCCTGAACGGCGTGACTTCCCTGGCTCATGCAACCACCAACTGCTTCCCAATGATCCTGTTGAGCGGTTCCAGTGAACGTGAAATCGTCGATTTGCAACAGGGCGATTACGAAGAAATGGATCAGATGAATGTTGCACGTCCACACTGCAAAGCTTCTTTCCGTATCAACAGCATCAAAGACATTCCAATCGGTATCGCTCGTGCAGTTCGCACCGCTGTATCCGGACGTCCAGGTGGTGTTTACGTTGACTTGCCAGCAAAACTGTTCGGTCAGACCATTTCTGTAGAAGAAGCTAACAAACTGCTCTTCAAACCAATCGATCCAGCTCCGGCACAGATTCCTGCTGAAGATGCTATCGCTCGCGCTGCTGACCTGATCAAGAACGCCAAACGTCCAGTTATCATGCTGGGTAAAGGCGCTGCATACGCACAATGCGACGACGAAATCCGCGCACTGGTTGAAGAAACCGGCATCCCATTCCTGCCAATGGGTATGGCTAAAGGCCTGCTGCCTGACAACCATCCACAATCCGCTGCTGCAACCCGTGCTTTCGCACTGGCACAGTGTGACGTTTGCGTACTGATCGGCGCTCGTCTGAACTGGCTGATGCAGCACGGTAAAGGCAAAACCTGGGGCGACGAACTGAAGAAATACGTTCAGATCGACATCCAGGCTAACGAAATGGACAGCAACCAGCCTATCGCTGCACCAGTTGTTGGTGACATCAAGTCCGCCGTTTCCCTGCTCCGCAAAGCACTGAAAGGCGCTCCAAAAGCTGACGCTGAATGGACCGGCGCTCTGAAAGCCAAAGTTGACGGCAACAAAGCCAAACTGGCTGGCAAGATGACTGCCGAAACCCCATCCGGAATGATGAACTACTCCAATTCCCTGGGCGTTGTTCGTGACTTCATGCTGGCAAATCCGGATATTTCCCTGGTTAACGAAGGCGCTAATGCACTCGACAACACTCGTATGATTGTTGACATGCTGAAACCACGCAAACGTCTTGACTCCGGTACCTGGGGTGTTATGGGTATTGGTATGGGCTACTGCGTTGCTGCAGCTGCTGTTACCGGCAAACCGGTTATCGCTGTTGAAGGCGATAGCGCATTCGGTTTCTCCGGTATGGAACTGGAAACCATCTGCCGTTACAACCTGCCAGTTACCGTTATCATCATGAACAATGGTGGTATCTATAAAGGTAACGAAGCAGATCCACAACCAGGCGTTATCTCCTGTACCCGTCTGACCCGTGGTCGTTACGACATGATGATGGAAGCATTTGGCGGTAAAGGTTATGTTGCCAATACTCCAGCAGAACTGAAAGCTGCTCTGGAAGAAGCTGTTGCTTCCGGCAAACCATGCCTGATCAACGCGATGATCGATCCAGACGCTGGTGTCGAATCTGGCCGTATCAAGAGCCTGAACGTTGTAAGTAAAGTTGGCAAGAAATAAGGTTCCTAGCTGATTAGAAGGCCATCCTGACGGATGGCCTTTTTTTTGACTCTGTTGTGTCTTCTTTTAGGTGTGGAATAATTTTCCAACCATCACTTTATCTGTCTCCCGAACAGCACCTTATTCAGCCATCGGTTCACATACACGTTCACGAAAGCACATCCTACTCCGATAGCGGCTCCTGCCACTACATCACTGGGATAGTGCACCCCTTGGTTCATCCGGGCAAACCCTACTCCGCAAGCCCATACGGCTGAAGGGGCGATAACATACCACTTAGGATAAGTGATGGAGAGTGAGGTGGCTAAAGAAAAAGCTGCCGCAGTGTGTCCTGAAGGGAATGACGGACTATCGGCATCCGGTGCTCCGTATGCATGAATCTTATCCGGATACTTTACAAAAGGCCGGTCACGACCTATTATATGTTTGGCGGCGTATGTAATCCCCACTGCTTCAATCACACTGGTTCCTATGTAAATGGCATCTTTCAGCATCGGCTGGTCTTTCTTTATCAATGCATAGAGTCCCATGGCTGTAGGTATTCCTACGGGTAGAATCACTCCGCTAGCGGACAATCCGCGGCTCAGTCCGTGTACATCCCATCGGTTGATGGTTTTCAACGTGTTGATGTCTATATTCTGTGCGCTGAGGCTGAATGTAAAACAGGCTGTCAGTATAAGAAAAATAATCCTTTGGCGTTTCATTTAGTTTGGCATTTGTTCAGTGCAAAGATAGGAAAATAATGTAATTCGCATTTACCCTTCCCCGAATTAAAGAATAATTTCCATAATTCCTTGTATTATCGCTAGAATTCCTTTACTTTTGCATCGTGAATACACAGCTTTTAACAGAATTTTTAGTTCTAACAATTAAATAATTTAAATTCAATCATTTATGTGGTTAAGTAATTCATCTATTGGAAGGAAAGTGGTGATGAGTGTCACAGGTATCGCCCTTGTCCTGTTTCTGACGTTTCACATGGCGATGAACCTTGTTGCGCTCTTCTCGGAAGATGCTTACAACATGATTTGTGAATTCTTGGGAGCTAACTGGTACGCGTTGGTTGCAACTGCCGGGCTGGCTGCTCTGTTTGTAATTCACATCATCTACGCTTTCTGGTTGACCATGCAGAACCGTGCAGCTCGCGGTAGCGAACGTTATGCAGTGAACGTTAAACCTAAAACTGTGGAATGGGCATCTCAGAACATGCTTGTCCTGGGTATTGTCGTTATTGCCGGATTGGCTCTTCATTTTGCCAATTTCTGGTACAAAATGCAGTTTGCCGAAATTATCGGTGTTCATGATTTGGGCGCATTCGGTCCTACAGACGGTGCCGCTTACATTCGTGAAACATTCGGATGCCCTGTATTTACAGTGCTTTACCTCATTTGGTTAGCAGCTTTGTGGTTCCACCTGACTCACGGATTCTGGAGTGCTTTGCAGACTTTGGGCTGGAGCAATAAAATCTGGTTCGAGCGTTGGAAGACTATTGGTAACATCTACTCTACTTTGGTAGTTCTTGGTTTTGCAGCAGTAGTCGTTATCTACTTCGCTATGACTGGCGCGCCTGATAGGTGGGCTGCCCTTCCTGGTTGGCTTGGTTTCATCAGCCATCCGCTTGCCCTCATCTGTTACGCCGGCGGTAGCCGGCCAGCCTCGCAGAGCAGGATTCCCGTTGAGCACCGCCAGGTGCGAATAAGGGACAGTGAAGAAGGAACACCCGCTCGCGGGTGGGCCTACTTCACCTATCCTGCCCGGCTGACGCCGTTGGATACACCAAGGAAAGTCTACACGAACCCTTTGGCAAAATCCTGTATATCGTGCGAAAAAGGATGGATATACCGAAAAAATCGCTATAATGACCCCGAAGCAGGGTTATGCAGCGGAAAAGCGGGATTAAAAGTCGGGGATTGGTGAACAAAAAGGTGTTTCTCTCTTTAAGAGAAATATCGTTTTGCTAAACAGTTGATATTGAGGTATCATTTTATCGTAAAAGACATTTTTGCTCAACAATTGCTTGACGGAAATCAACAAATTTTAGCATTTTGTAAAAAAGTCGCTATATAATTTGGTGAATTGGAGTTATTTTCATATTTTTGCATCCCGAAGAGTTTCTCTTAAAGAGAGAAACATCTTTTGCATACCTTTTCCGACCGAATTTTTATGTCGTAAAGAGGGGCTTTGCAGGGGGTGGACTCAGAAAGATGAGAATAGATGACTATTGTAGTTGAAACACATAGAAAGTTGCTGATATACAGACCGATACGCATATCGGGATGAACCATGAGTACGTTCTTTTCTCAAAAAACATAAATATTCGAAAAGAGATGCAATAAATTAAGGAGAGGTTATAATGAACAAAGTAAATATAAAAGATAGTCAAAATTTTATTACTTCAAAATATCACATAGAAAAAATAATGAATTGCATAAGTTTAGATGAAAAAGATAACATCTTTGAAATAGGTGCAGGGAAAGGTCATTTTACTGCTGGATTGGTAAAGAGATGTAATTTTGTAACGGCGATAGAAATTGATTCTAAATTATGTGAGGTAACTCGTAATAAGCTCTTAAATTATCCTAACTATCAAATAGTAAATGATGATATACTGAAATTTACATTTCCTAGCCACAATCCATATAAAATATTTGGCAGCATACCTTACAACATAAGCACAAATATAATTCGAAAAATTGTTTTTGAAAGTTCAGCCACAATAAGTTATTTAATAGTGGAATATGGTTTTGCTAAAATGTTATTAGATACAAACAGATCACTAGCATTGCTGTTAATGGCAGAGGTAGATATTTCTATATTAGCAAAAATTCCTAGGTATTATTTCCATCCAAAACCTAAAGTGGATAGCACATTAATTGTATTAAAAAGAAAGCCAGCAAAAATGGCATTTAAAGAGAGAAAAAAATATGAAACTTTTGTAATGAAATGGGTTAACAAAGAGTACGAAAAACTGTTTACAAAAAATCAATTTAATAAAGCTTTAAAACATGCGAGAATATATGATATAAACAATATTAGTTTCGAACAATTTGTATCGCTATTTAATAGTTATAAAATATTTAACGGCTAAAAACAATAGGCCACATGCAACTGTAAATGTTTACGCGGGTACCGACACCGCGGTGGAGGGGAATTGTGTTACAACCAATTAACCAATTCTGATTAGAAAAACTCATCGAGCATCAAATGAAACTGCAATTTATTCATATCAGGATTATCAATACCATATTTTTGAAAAAGCCGTTTCTGTAATGAAGGAGAAAACTCACCGAGGCAGTTCCATAGGATGGCAAGATCCTGGTATCGGTCTGCGATTCCGACTCGTCCAACATCAATACAACCTATTAATTTCCCCTCGTCAAAAATAAGGTTATCAAGTGAGAAATCACCATGAGTGACGACTGAATCCGGTGAGAATGGCAAAAGCTTATGCATTTCTTTCCAGACTTGTTCAACAGGCCAGCCATTACGCTCGTCATCAAAATCACTCGCATCAACCAAACCGTTATTCATTCGTGATTGCGCCTGAGCGAGGCGAAATACGCGATCGCTGTTAAAAGGACAATTACAAACAGGAATCGAATGCAACCGGCGCAGGAACACTGCCAGCGCATCAACAATATTTTCACCTGAATCAGGATATTCTTCTAATACCTGGAATGCTGTTTTCCCGGGGATCGCAGTGGTGAGTAACCATGCATCATCAGGAGTACGGATAAAATGCTTGATGGTCGGAAGAGGCATAAATTCCGTCAGCCAGTTTAGTCTGACCATCTCATCTGTAACATCATTGGCAACGCTACCTTTGCCATGTTTCAGAAACAACTCTGGCGCATCGGGCTTCCCATACAATCGATAGATTGTCGCACCTGATTGCCCGACATTATCGCGAGCCCATTTATACCCATATAAATCAGCATCCATGTTGGAATTTAATCGCGGCCTGGAGCAAGACGTTTCCCGTTGAATATGGCTCATAACACCCCTTGTATTACTGTTTATGTAAGCAGACAGTTTTATTGTTCATGATGATATATTTTTATCTTGTGCAATGTAACATCAGAGATTTTGAGACACAACGTGGCTTTGTTGAATAAATCGAACTTTTGCTGAGTTGAAGGATCAGAGCCTACGTTCCGAATACGGTCAAAAAAAAGGCCATCCGTCAGGATGGCCTTCGCATTAATATGCCGCTTCGAATTCTTTTAGGAAGCGTGTATCGTTTTCAGAGAACATACGGAGGTCTTTCACCTGATATTTCAGGTTTGTGATACGCTCGATACCCATACCGAGTCCATAACCGCTGTATATTTTGCTGTCTATACCATTTGATTCAAGTACGTTCGGGTCTACCATACCGCAACCGAGGATTTCTACCCAGCCGGTGTGTTTACAGAACGGACATCCTTTACCGCCGCAGATATTACAGCTGATATCCATTTCCGCACTTGGTTCAGCAAACGGGAAGTAAGACGGACGCAGACGGATCTTTGTATCAGCACCGAACATTTCTTTGGCAAAGAGCAGCAATACCTGCTTCAAGTCGGTGAATGATACGTTTTTATCTACATACAGCGCTTCTACCTGATGGAAGAAACAGTGTGCGCGATAGCTGATAGCTTCGTTACGATATACACGTCCCGGACAGATGATGCGGATAGGAGGCTGTGAAGTTTCCATCACACGAGTCTGTACAGAAGAAGTATGTGTACGCAATACTACGTCCGGGTGAGCTTCGATAAAGAAAGTGTCCTGCATATCGCGTGCCGGATGATCTTCGGCAAAGTTCAGTGCCGAGAACACGTGCCAGTCATCTTCAATTTCCGGACCTTCGGCAATGCTGAATCCCAGACGGGCAAAGATATCAATGATTTCGTTCTTTACAATGGTGAGCGGGTGGCGTGTACCGAGTTCTACAGGATAAGCCGAACGCGTCAAATCCAGTCCGTCACAATCGTTGTCCTGACTTTCAAACATTTCTTTCAGCGCGTTGATTTTGTCCTGCGCTTTTGTTTTCAGTTCATTCAGTCTCATGCCGACTTCTTTTTTCTGTTCGGCAGCTACATTACGGAAATCTGCCATTAAGTCGTTAATGGCTCCCTTCTTACTTAGGTATTTGATGCGGAGAGCTTCGAGTTCTTCGGCATTGGAGGCGTGTAAGGCTTCCACCTCTTTCAGAAGTTGTTCAATCTTAGCTATCATTTTTTAATATTTTTAGCGGCCCCGTTAAACAAAATTATTTGTAGAGGCTGTTTCGTCCTCACGGACTCATCAGACCGGAAAGCACATCCGGTGACAGCTCAGGCTACTTTGTTTCTTTCGACACTGCAAATATAAGAACATTATTTGAAAGTTCAAGTGAAACTTTAAATTTTAACAATAGATTAACCATTGCAAACAAAACAAAAAAAAGGTAGCCCAATTGTAAAACGAAAGGCCCAGTCTTTCGACTGAGCCTTTCGTTTTATCCTACAGTCGCTCGGCGATCGAAGGCTTCGGAAAAAAAAGGCCATCCGTCAGGATGGCCTTCGCATTAATATGCCGCTTCGAATTCTTTTAGGAAGCGTGTATCGTTTTCAGAGAACATACGGAGGTCTTTCACCTGATATTTCAGGTTTGTGATACGCTCGATACCCATACCGAGTCCATAACCGCTGTATATTTTGCTGTCTATACCATTTGATTCAAGTACGTTCGGGTCTACCATACCGCAACCGAGGATTTCTACCCAGCCGGTGTGTTTACAGAACGGACATCCTTTACCGCCGCAGATATTACAGCTGATATCCATTTCCGCACTTGGTTCAGCAAACGGGAAGTAAGACGGACGCAGACGGATCTTTGTATCAGCACCGAACATTTCTTTGGCAAAGAGCAGCAATACCTGCTTCAAGTCGGTGAATGATACGTTTTTATCTACATACAGCGCTTCTACCTGATGGAAGAAACAGTGTGCGCGATAGCTGATAGCTTCGTTACGATATACACGTCCCGGACAGATGATGCGGATAGGAGGCTGTGAAGTTTCCATCACACGAGTCTGTACAGAAGAAGTATGTGTACGCAATACTACGTCCGGGTGAGCTTCGATAAAGAAAGTGTCCTGCATATCGCGTGCCGGATGATCTTCGGCAAAGTTCAGTGCCGAGAACACGTGCCAGTCATCTTCAATTTCCGGACCTTCGGCAATGCTGAATCCCAGACGGGCAAAGATATCAATGATTTCGTTCTTTACAATGGTGAGCGGGTGGCGTGTACCGAGTTCTACAGGATAAGCCGAACGCGTCAAATCCAGTCCGTCACAATCGTTGTCCTGACTTTCAAACATTTCTTTCAGCGCGTTGATTTTGTCCTGCGCTTTTGTTTTCAGTTCATTCAGTCTCATGCCGACTTCTTTTTTCTGTTCGGCAGCTACATTACGGAAATCTGCCATTAAGTCGTTAATGGCTCCCTTCTTACTTAGGTATTTGATGCGGAGAGCTTCGAGTTCTTCGGCATTGGAGGCGTGTAAGGCTTCCACCTCTTTCAGAAGTTGTTCAATCTTAGCTATCATTTTTTAATATTTTTAGCGGCCCCGTTAAACAAAATTATTTGTAGAGGCTGTTTCGTCCTCACGGACTCATCAGACCGGAAAGCACATCCGGTGACAGCTCAGGCTACTTTGTTTCTTTCGACACTGCAAATATAAGAACATTATTTGAAAGTTCAAGTGAAACTTTAAATTTTAACAATAGATTAACCATTGCAAACAAAACAAAAAAAAGGTAGCCCAATTGTAAAACGAAAGGCCCAGTCTTTCGACTGAGCCTTTCGTTTTATCCTAGGATCAGCTGTACGTACTCGCAGTTCAACCTGTTGATAGTACGTACTAAGCTCTCATGTTTCACGTACTAAGCTCTCATGTTTAACGTACTAAGCTCTCATGTTTAACGAACTAAACCCTCATGGCTAACGTACTAAGCTCTCATGGCTAACGTACTAAGCTCTCATGTTTCACGTACTAAGCTCTCATGTTTGAACAATAAAATTAATATAAATCAGCAACTTAAATAGCCTCTAAGGTTTTAAGTTTTATAAGAAAAAAAAGAATATATAAGGCTTTTAAAGCTTTTAAGGTTTAACGGTTGTGGACAACAAGCCAGGGATGTAACGCACTGAGAAGCCCTTAGAGCCTCTCAAAGCAATTTTGAGTGACACAGGAACACTTAACGGCTGACATGGGGCGGCCGCGTAG
SEQ ID NO:86(用于基因组整合进普通拟杆菌基因组中的编码草酸盐降解途径的质粒)：
GGAGAGCTGTATGTGGGGTGGAAGAAGCTATCGTAAAAATGCTGGAGCAGCTTGATCCTCATTCTACCTATTCTGATCCGGAGGAAGTGAAGAGAATGAACGAGCCGTTGCAGGGAAACTTCGAAGGTATCGGTATACAGTTTAATATGGCGGAGGACACTTTGCTGGTGATACAGCCTGTATCGGGCGGACCGTCCGAAAAAGTCGGTATTCTGGCCGGTGATCGTATCGTCATGGTGGAAGATACGCTGATAGCCGGTGTAAAGATGAGTACCGAGGATATTATGCGTCGCCTCCGTGGTCCTAAAGATTCAAAAGTGAATCTGAAAATACTTCGCCGAGGGGTGAAGGAACTGCTCCCCTTTACGGTGAAAAGGGATAAAATACCTGTTTACAGCTTGGATGCTTCCTATATGATTAAAGATAAGATCGGTTATATTCGTATCAACCGTTTTGCCGCCACTACCCATGAGGAGTTCAAGAAAGCGCTGACCGGGTTGCAGAAGAAAGGAATGAAAGACTTGATTCTTGATTTGCAGGGCAATGGCGGGGGGTATTTGAATGCTGCTATCGATATTGCCAATGAATTTTTGGGTAAAAAGGAACTGATTGTCTATACGGAGGGCCGTCGTAATCCTCGTAGCGAGTTTTTTGCCAAAGGTACGGGCGAGTTTCAGAACGGTCGTCTGATGGTATTGGTTGATGAATTTTCGGCTTCTGCCAGTGAGATTGTGACCGGAGCTGTTCAGGATTGGGACAGAGGCGTTGTGGTAGGCCGTCGCAGTTTTGGTAAAGGCTTGGTGCAGCGTCCCATTGATTTGCCGGACGGTTCGATGATCCGTCTGACGGTGGCCCGCTATTATACGCCTGCCGGACGTTGTATCCAGAAACCTTACGAGAGCATTGAAAAGTATAATGAAGACCTGATAGACCGTTATAATAAAGGAGAAATGATGAGTGAAGACAGCATCCACTTTCCGGATTCACTGAAGTTCAAGACTCATAGGTTGGCCCGTACGGTATATGGTGGCGGAGGGATCATGCCCGATTATTTTGTGCCGATTGATACGACTCTCTATACTAAATACCATCGTCAGTTGCGTGATAAAGGAGCATTGATGAAAGCTCATTTCCATTTTATTGATGCCCATCGTAAAGAATGGCTGGGCAAATACAAGACTTTCAATGAGTTCTACAAACGTTTTGAAGTGACTCCGGATATGTTGACACAACTGGTTGCTACCGGCAAGGAGATGGGAGTGGAATATAATGAAGAAGAGTATCAAAAGGCCCTGCCTCTGCTGAGGTTGCAGATGAAAGCATTGATAGCCCGTGATTTATGGGATATGAATGAATATTATCACGTCATTAATGACGCGAATGAAAGTATCAGGAAAGCGCTGGAACTGTTGGAGCAACCGGACTTTGAGGGGCTGCTGTTGAAAAAGCGATAGTCAAGACTTTATATTAACTGCAGGGGCGCATAGTTGCACTCCTGCAGTTGTCTGAAAAACTTGTAGGATAAAACGAAAGGCTCAGTCGAAAGACTGGGCCTTTCGTTTTACAATTGGGCTACCTTTTTTTTGTTTTGTTTGCAATGGTTAATCTATTGTTAAAATTTAAAGTTTCACTTGAACTTTCAAATAATGTTCTTATATTTGCAGTGTCGAAAGAAACAAAGTAGCCTGGCATCACCTAAAAATTATAAATAAATTAATAAACTTTTATTAAAAAAAAATTACAAAATGAATAATCCACAAACAGGACAATCAACAGGCCTCTTGGGCAATCGTTGGTTCTACTTGGTATTAGCAGTTTTGCTGATGTGTATGATCTCGGGTGTCCAATATTCCTGGACACTGTACGCTAACCCGGTTAAAGACAACCTTGGCGTTTCTTTGGCTGCGGTTCAGACGGCTTTCACACTCTCTCAGGTCATTCAAGCTGGTTCTCAGCCTGGTGGTGGTTACTTCGTTGATAAATTCGGTCCAAGAATTCCATTGATGTTCGGTGGTGCGATGGTTCTCGCTGGCTGGACCTTCATGGGTATGGTTGACAGTGTTCCTGCTCTGTATGCTCTTTATACTCTGGCCGGTGCAGGTGTTGGTATCGTTTACGGTATCGCGATGAACACGGCTAACAGATGGTTCCCGGACAAACGCGGTCTGGCTTCCGGTTTCACCGCTGCCGGTTACGGTCTGGGTGTTCTGCCGTTCCTGCCACTGATCAGCTCCGTTCTGAAAGTTGAAGGTGTTGGCGCAGCATTCATGTACACCGGTTTGATCATGGGTATCCTGATTATCCTGATCGCTTTCGTTATCCGTTTCCCTGGCCAGCAAGGCGCCAAAAAACAAATCGTTGTTACCGACAAGGATTTCAATTCTGGCGAAATGCTGAGAACACCACAATTCTGGGTTCTGTGGACCGCATTCTTTTCCGTTAACTTTGGTGGTTTGCTGCTGGTTGCCAACAGCGTCCCTTACGGTCGCAGCCTCGGTCTTGCCGCAGGTGTGCTGACGATCGGTGTTTCGATCCAGAACCTGTTCAATGGTGGTTGCCGTCCTTTCTGGGGTTTCGTTTCCGATAAAATCGGCCGTTACAAAACCATGTCCGTCGTTTTCGGTATCAATGCTGTTGTTCTCGCACTTTTCCCGACGATTGCTGCCTTGGGCGATGTAGCCTTTATCGCCATGTTGGCAATCGCATTCTTCACATGGGGTGGTAGCTACGCTCTGTTCCCATCGACCAACAGCGATATTTTCGGTACGGCATACTCTGCCAGAAACTATGGTTTCTTCTGGGCTGCAAAAGCAACTGCCTCGATCTTCGGTGGTGGTCTGGGTGCTGCAATTGCAACCAACTTCGGATGGAATACCGCTTTCCTGATTACTGCGATTACTTCTTTCATCGCATTTGCTCTGGCTACCTTCGTTATTCCAAGAATGGGCCGTCCAGTCAAGAAAATGGTCAAATTGTCTCCAGAAGAAAAAGCTGTACATTAAGGTTCCTAACCTGCCTGGCATCTTAATCAATTTTAAACTCACCAAATCAAAATATGAAAAAAAAAAATAATAAAATGTCAACTCCACTTCAAGGAATTAAAGTTCTCGATTTCACCGGTGTGCAATCTGGCCCATCTTGTACTCAAATGCTGGCCTGGTTTGGCGCTGACGTTATTAAAATTGAACGTCCCGGCGTTGGTGACGTAACGCGTCACCAGCTGCGAGATATTCCTGATATCGATGCGCTTTACTTCACCATGCTTAACAGTAACAAACGTTCTATTGAGTTAAATACCAAAACAGCGGAAGGCAAAGAGGTAATGGAAAAGCTGATCCGCGAAGCTGATATCTTAGTCGAGAACTTTCATCCAGGGGCCATTGATCACATGGGCTTCACCTGGGAGCATATTCAAGAAATCAATCCACGTCTGATTTTTGGTTCGATCAAAGGGTTTGATGAGTGTTCGCCTTATGTGAATGTAAAAGCCTATGAAAACGTTGCTCAGGCAGCGGGTGGCGCGGCATCCACTACGGGTTTTTGGGATGGTCCGCCGCTGGTAAGCGCTGCAGCGTTGGGTGACAGCAACACCGGAATGCATTTGCTGATCGGTTTACTTGCTGCTTTGCTGCATCGCGAAAAAACGGGGCGTGGGCAACGAGTCACCATGTCAATGCAGGATGCCGTATTGAACCTTTGCCGCGTGAAATTACGTGACCAGCAGCGTCTAGATAAATTGGGTTATCTGGAAGAATACCCGCAGTATCCGAATGGTACATTTGGTGATGCAGTTCCCCGCGGTGGTAATGCAGGTGGTGGCGGTCAGCCTGGCTGGATCCTGAAATGTAAAGGCTGGGAAACCGATCCTAACGCCTATATTTATTTCACTATTCAGGAGCAAAACTGGGAAAACACCTGTAAAGCCATCGGCAAACCAGAATGGATTACCGATCCGGCATACAGTACAGCCCATGCACGACAGCCACATATTTTCGATATTTTTGCTGAAATCGAAAAATACACTGTCACTATTGATAAACATGAAGCGGTGGCCTATTTGACTCAGTTTGATATTCCTTGTGCACCGGTTTTAAGTATGAAAGAAATTTCACTTGATCCCTCTTTGCGCCAAAGTGGCAGTGTTGTTGAAGTGGAACAACCGTTGCGTGGAAAATATCTGACCGTTGGTTGTCCAATGAAATTCTCTGCCTTTACGCCGGATATTAAAGCTGCGCCGCTATTAGGTGAACATACCGCTGCTGTATTGCAGGAGCTGGGTTATAGCGACGATGAAATTGCTGCAATGAAGCAAAACCACGCCATCTGAGGTTCCTAAACGACCTGGCATCAACTACCTTTATTATTCAACAAACCAATTTAACATATTAAAAAAATAAAAAAATGACTACCACAGCAACGCAGCATTTTGCCCAGTTACTCCAGAAGTACGGAATTGATACTGTCTTCGGCATAGTAGGTATTCCGATCGTGCAGTTGGCCGACACTATGGTCGCGAATGGAATTAAGTTTATACCGTGTCGCAATGAGCAAGCCGCAAGTTACGCGGCAAGCGCCTACGGCTACATCTCGGACAAACCTGGTGTCCTGTTAATCGTGGGAGGGCCTGGCCTTATTCATGCCTTAGCGGGGATCTATAATAGTATGTCGAACCGCTGGCCCTTGTTGGTCATCGCCGGATCCAGCAGCCAGTCAGACATCCACAAGGGAGGTTTCCAGGAACTTGACCAGGTGTCCTTGCTAAGTCCGTTCCTAAAATTCACTGGTAAACTGACGCCGGATAACATCGATATGATAACCCAAAAAGCACTCAACTATTGCATTCAGGGAACCGCCGGAGTGAGCTATATCGATGTCCCCGCTGACTTCATCGAATACGAGAAGCCGCTTGAGGGCAATGATAGAACTGGTAACGAGCTGCCAATGATCCTGACGCCCAATATCTGCGGTCCAGACCCCAGTAAAATTAAGAAGGTGGTGCAATTGATACTCCAGCACAAGAACAAGAACATATTAATAGTGATAGGTAAAGGAGCAGTGAAGAATAGCCATGAGATTAGACGCTTGGTTAACACCTTTAACTTGCCGTTCCTTCCGACTCCCATGGCGAAAGGCATAGTGCCCGATAGCAGCCCTTTAAATGTGAGCAGCGCCCGGAGCCAGGCTCTTAAGATTGCTGACATCGTGTTAGTATTGGGAGCTCGCCTGAATTGGATCCTTCACTTCGGAACGAGTCCAAAGTGGAATAGCGAGAGTATTTTTATACAATTTGACAGTAACCCGGAAACGCTCGGAGACAATAACGTTAGTCCCGGAGCAGATTTAAGTATCTGGGGGGACATCGGACTATCGGTTACTGCACTGGTAGAAGAGCTCACGAGACAGGACAGTTGTTGGAAATACTCGGGAGTCAAACAGGAGATCAGGGAAAAGATACAGTTAAACCAGACCAGATTGCTCCGTAAGGAAAAAACGCGTGGAGCTCAGCTTAACTATAATCAAGTGTACGGCACCCTTCGTCCGTTAATTGACGACTATCGCACGATCTTGGTCACAGAAGGTGCCAACACAATGGACATCGCTAGAATAAGTTTTCCGACTGATGCACCTCGCAGAAGACTTGATGCTGGTACAAATGCTACAATGGGTATAGGTTTAGGTTACGCATTAGCGTGTAAAGCCAGCCACCCTGAGTTAGACGTTGTTTTAATCCAAGGCGACAGTGCCTTCGGTTTTAGCGCTATGGAGATCGAGACTGCCGTCCGTTGCCAATTAGCCCTTGTGATTGTCGTAATGAATAACTCTGGGATATATCACGGTGAGAAAGACATCGAAGGAGATCTGCCCCCGACCGCTTTGTCAAAAAATTGTAGATATGACTTGGTCGGTAAGGGTTTAGGAGCTAACGACTTCTTCGTCAATACCATCTCAGAGTTATCTCGCTGTTTTCAGCAGGCAGTTCAATTGAGCAGAACCAAGCGCGAAACCTCCGTCATTAATGTCATAATCGAACCGGGAGAGCAGAAACAGATCGCGTTCGCATGGCAAAACAAGCCTAGACTGTAAGGTTCCTAAGGGTCCTGGCATCAACTACCTAAATTACTTATCAAACCACTTAGTAGTAAAAAAAATATAACAAAATGACAAATAATGAAAGCAAAGGGCCGTTTGAAGGCTTATTAGTTATCGATATGACACATGTCCTTAATGGACCTTTCGGAACTCAACTTCTTTGTAATATGGGCGCAAGGGTAATTAAAGTTGAGCCGCCGGGTCATGGTGATGATACCCGCACATTTGGTCCCTATGTGGATGGACAGTCACTCTATTACAGTTTTATTAATCATGGCAAAGAGAGTGTGGTTCTTGATTTAAAGAATGATCACGATAAAAGTATATTTATAAATATGCTCAAACAAGCTGATGTATTAGCTGAGAATTTTCGCCCAGGTACAATGGAAAAACTGGGGTTTTCATGGGAAACGCTTCAAGAAATCAACCCGCGCCTCATATATGCTTCATCGTCAGGTTTCGGACATACCGGTCCGCTAAAAGATGCTCCTGCCTACGATACCATCATTCAGGCAATGAGCGGGATAATGATGGAAACAGGATATCCTGATGCTCCGCCAGTGCGCGTTGGTACATCTCTTGCGGATCTATGCGGCGGTGTCTATTTATTCAGCGGAATAGTGAGTGCACTTTATGGCCGCGAAAAGAGCCAGAGAGGGGCGCATGTCGATATAGCGATGTTTGATGCCACGCTGAGTTTTCTGGAGCATGGTCTGATGGCATATATCGCAACTGGGAAGTCACCACAACGTCTGGGAAATCGCCATCCCTACATGGCACCTTTTGATGTTTTCAATACTCAGGATAAGCCGATTACGATTTGTTGTGGTAATGACAAGCTTTTTTCTGCGTTATGCCAGGCACTGGAGCTTACGGAACTGGTTAATGATCCCCGATTTAGCAGCAATATTTTACGCGTACAAAACCAGGCTATTCTTAAACAATATATTGAGCGGACGTTAAAAACGCAGGCAGCTGAAGTTTGGTTAGCCAGAATACATGAAGTTGGTGTACCCGTCGCGCCGTTATTAAGTGTGGCTGAGGCCATTAAATTGCCACAAACTCAGGCGAGAAATATGTTGATTGAAGCCGGGGGAATAATGATGCCGGGTAATCCGATAAAAATCAGCGGCTGCGCGGACCCGCATGTTATGCCGGGAGCGGCAACGCTCGACCAGCATGGGGAACAAATTCGCCAGGAGTTCTCATCATAAGGTTCCTAATTCCCCTGGCATCTTTTCCATAACAAAAATTAAACCTCCTCCATATGAAAAATAAAAATTATAAAATGACAAGTGCCGCTACTGTTACTGCTTCGTTCAACGATACTTTTAGCGTATCCGATAATGTCGCCGTTATTGTTCCTGAAACGGACACTCAGGTGACCTACAGGGATCTATCCCACATGGTGGGTCACTTCCAGACCATGTTCACAAATCCTAATTCTCCATTGTACGGAGCTGTTTTCAGACAAGATACAGTGGCGATATCCATGCGTAATGGGCTGGAATTTATCGTCGCTTTCCTCGGTGCTACTATGGACGCTAAAATTGGCGCGCCCTTGAATCCCAATTATAAGGAAAAGGAGTTCAATTTTTATTTGAATGACCTGAAATCTAAGGCGATTTGCGTCCCAAAGGGTACCACAAAGTTACAGAGTTCTGAAATTCTAAAATCTGCCTCCACGTTTGGATGTTTTATCGTAGAGCTGGCCTTCGATGCGACCAGGTTTAGGGTAGAGTATGATATATACTCTCCAGAGGACAACTACAAAAGGGTTATTTACCGGTCTTTGAACAACGCCAAATTTGTCAACACAAATCCCGTTAAATTCCCTGGGTTTGCCCGTTCCAGTGACGTTGCCCTGATTTTGCATACCAGTGGTACCACCTCCACTCCAAAAACGGTGCCTTTGTTACATTTGAACATTGTGAGAAGCACGTTGAACATTGCTAACACTTACAAGCTAACGCCCTTGGACAGATCTTATGTCGTGATGCCTCTTTTCCACGTCCATGGGTTAATTGGTGTTTTACTTTCCACTTTTAGAACTCAGGGTTCTGTTGTGGTTCCCGATGGATTCCATCCAAAGTTATTCTGGGACCAATTTGTTAAGTACAACTGTAATTGGTTCAGTTGCGTTCCCACAATAAGCATGATTATGCTGAACATGCCCAAACCAAACCCTTTCCCACACATTAGATTCATCAGATCGTGTTCTTCTGCTTTGGCTCCAGCAACGTTCCATAAGCTGGAGAAGGAATTCAATGCACCTGTCTTGGAGGCCTATGCGATGACCGAAGCATCACATCAAATGACCTCAAACAATCTGCCTCCAGGAAAGAGAAAGCCTGGTACTGTGGGCCAGCCACAAGGAGTCACCGTCGTCATTCTAGATGACAATGACAATGTCTTGCCCCCGGGCAAAGTCGGCGAAGTTTCCATCAGAGGCGAAAACGTCACTTTGGGGTATGCTAATAATCCAAAAGCTAACAAGGAGAACTTCACCAAGAGAGAGAACTATTTCAGAACCGGTGACCAAGGTTATTTCGACCCTGAGGGGTTTTTGGTCCTTACAGGCAGAATCAAAGAGCTTATCAACAGGGGTGGTGAAAAGATTTCACCCATTGAGCTCGACGGCATTATGCTATCGCATCCAAAGATCGATGAAGCCGTTGCATTTGGTGTTCCCGACGATATGTACGGCCAAGTAGTTCAAGCCGCCATTGTTTTGAAGAAGGGAGAAAAAATGACCTACGAAGAACTGGTGAACTTCTTAAAGAAGCACCTAGCCTCTTTCAAAATTCCAACCAAGGTGTACTTTGTTGATAAGCTACCAAAAACCGCTACAGGTAAAATCCAGAGAAGAGTTATCGCAGAAACTTTTGCTAAGAGCAGCAGAAATAAGAGTAAGTTGTAGGGTTCCTAAAATGCCTGGCATCAACTACCTAAATTAATTATCACCCCAAATTAATAACATAAAAATATAATAAAATGAGTAACGACGACAATGTAGAGTTGACTGATGGCTTTCATGTTTTGATCGATGCCCTGAAAATGAATGACATCGATACCATGTATGGTGTTGTCGGCATTCCTATCACGAACCTGGCTCGTATGTGGCAAGATGACGGTCAGCGTTTTTACAGCTTCCGTCACGAACAACACGCAGGTTATGCAGCTTCTATCGCCGGTTACATCGAAGGAAAACCTGGCGTTTGCTTGACCGTTTCCGCCCCTGGCTTCCTGAACGGCGTGACTTCCCTGGCTCATGCAACCACCAACTGCTTCCCAATGATCCTGTTGAGCGGTTCCAGTGAACGTGAAATCGTCGATTTGCAACAGGGCGATTACGAAGAAATGGATCAGATGAATGTTGCACGTCCACACTGCAAAGCTTCTTTCCGTATCAACAGCATCAAAGACATTCCAATCGGTATCGCTCGTGCAGTTCGCACCGCTGTATCCGGACGTCCAGGTGGTGTTTACGTTGACTTGCCAGCAAAACTGTTCGGTCAGACCATTTCTGTAGAAGAAGCTAACAAACTGCTCTTCAAACCAATCGATCCAGCTCCGGCACAGATTCCTGCTGAAGATGCTATCGCTCGCGCTGCTGACCTGATCAAGAACGCCAAACGTCCAGTTATCATGCTGGGTAAAGGCGCTGCATACGCACAATGCGACGACGAAATCCGCGCACTGGTTGAAGAAACCGGCATCCCATTCCTGCCAATGGGTATGGCTAAAGGCCTGCTGCCTGACAACCATCCACAATCCGCTGCTGCAACCCGTGCTTTCGCACTGGCACAGTGTGACGTTTGCGTACTGATCGGCGCTCGTCTGAACTGGCTGATGCAGCACGGTAAAGGCAAAACCTGGGGCGACGAACTGAAGAAATACGTTCAGATCGACATCCAGGCTAACGAAATGGACAGCAACCAGCCTATCGCTGCACCAGTTGTTGGTGACATCAAGTCCGCCGTTTCCCTGCTCCGCAAAGCACTGAAAGGCGCTCCAAAAGCTGACGCTGAATGGACCGGCGCTCTGAAAGCCAAAGTTGACGGCAACAAAGCCAAACTGGCTGGCAAGATGACTGCCGAAACCCCATCCGGAATGATGAACTACTCCAATTCCCTGGGCGTTGTTCGTGACTTCATGCTGGCAAATCCGGATATTTCCCTGGTTAACGAAGGCGCTAATGCACTCGACAACACTCGTATGATTGTTGACATGCTGAAACCACGCAAACGTCTTGACTCCGGTACCTGGGGTGTTATGGGTATTGGTATGGGCTACTGCGTTGCTGCAGCTGCTGTTACCGGCAAACCGGTTATCGCTGTTGAAGGCGATAGCGCATTCGGTTTCTCCGGTATGGAACTGGAAACCATCTGCCGTTACAACCTGCCAGTTACCGTTATCATCATGAACAATGGTGGTATCTATAAAGGTAACGAAGCAGATCCACAACCAGGCGTTATCTCCTGTACCCGTCTGACCCGTGGTCGTTACGACATGATGATGGAAGCATTTGGCGGTAAAGGTTATGTTGCCAATACTCCAGCAGAACTGAAAGCTGCTCTGGAAGAAGCTGTTGCTTCCGGCAAACCATGCCTGATCAACGCGATGATCGATCCAGACGCTGGTGTCGAATCTGGCCGTATCAAGAGCCTGAACGTTGTAAGTAAAGTTGGCAAGAAATAAGGTTCCTAAAAACCCTGGCATCTAAACTAAAATACAATAACCAAAAATCATTACAAATGAAAAAAAATAATAAAATGGATAGCGATACTCTCTCAGGATTATTGGAAAACGTCGCCAAAAAATTCCCCGATCGCCGAGCTCTCTCCGTTTCTGGAAAATTCAATCTCACTCACGCGCGTCTTCACGATCTAATCGAACGCGCCGCTTCACGCCTTGTCTCCGACGCTGGAATCAAACCCGGCGATGTCGTTGCTCTCACCTTCCCTAACACCGTCGAGTTTGTTATAATGTTTTTAGCGGTGATAAGAGCTAGAGCCACGGCGGCGCCGTTGAACGCAGCGTACACGGCGGAGGAATTTGAGTTTTACCTCTCCGATTCAGATTCAAAGCTATTGTTAACCTCTAAAGAAGGAAACGCACCGGCTCAAGAAGCAGCTTCAAAGCTGAAAATCTCTCACGTCACCGCTACGCTGCTTGACGCTGGCTCGGACCTTGTACTATCCGTTGCGGATTCAGATTCCGTCGTTGACTCAGCGACGGAACTCGTTAATCACCCGGACGACGGTGCTCTCTTCCTCCACACTTCTGGCACTACGAGCCGTCCAAAGGGTGTACCGCTTACGCAGCTCAATCTAGCTTCATCCGTCAAGAACATTAAAGCTGTGTACAAGCTTACTGAGTCTGATTCTACGGTGATTGTTCTCCCTCTGTTCCATGTTCATGGATTGTTAGCTGGGTTGCTTAGCTCGCTTGGAGCTGGTGCTGCTGTAACTCTTCCAGCTGCTGGTAGATTCTCAGCAACAACATTTTGGCCAGATATGAAGAAGTATAACGCTACATGGTATACTGCTGTGCCGACCATTCATCAGATCATATTGGACCGCCACGCGAGCCACCCTGAAACGGAATATCCTAAACTCCGGTTCATCAGGAGTTGCAGTGCTTCTTTGGCTCCGGTGATATTGTCCAGGCTTGAGGAAGCGTTTGGAGCACCGGTGCTCGAGGCCTATGCAATGACAGAGGCAACACATTTGATGAGCTCAAACCCCTTACCAGAGGAAGGTCCACACAAGCCTGGGTCTGTTGGGAAACCGGTAGGTCAAGAAATGGCGATCCTTAATGAGAAAGGCGAGATCCAAGAGCCAAATAACAAAGGAGAGGTTTGTATAAGAGGTCCAAATGTGACCAAGGGTTACAAGAATAACCCAGAAGCCAACAAGGCAGGTTTCGAGTTTGGGTGGTTCCACACTGGTGATATCGGTTACTTTGATACCGATGGGTATTTGCATCTGGTGGGTCGGATCAAAGAGCTTATTAACCGTGGAGGTGAGAAGATATCTCCAATTGAAGTGGATGCAGTACTCTTAACGCATCCTGACGTTTCTCAGGGTGTTGCATTCGGTGTTCCTGATGAGAAATATGGGGAAGAGATTAACTGTGCGGTGATTCCAAGAGAAGGAACTACTGTAACCGAAGAGGACATTAAAGCGTTTTGTAAGAAGAATTTGGCAGCTTTCAAGGTGCCAAAGAGAGTGTTCATCACTGATAACCTCCCCAAAACTGCCTCTGGTAAGATTCAGCGCCGTATCGTCGCACAACATTTCCTTGAGAAGCCCTGAGGTTCCTAGCTGATTAGAAGGCCATCCTGACGGATGGCCTTTTTTTTGACTCTGTTGTGTCTTCTTTTAGGTGTGGAATAATTTTCCAACCATCACTTTATCTGTCTCCCGAACAGCACCTTATTCAGCCATCGGTTCACATACACGTTCACGAAAGCACATCCTACTCCGATAGCGGCTCCTGCCACTACATCACTGGGATAGTGCACCCCTTGGTTCATCCGGGCAAACCCTACTCCGCAAGCCCATACGGCTGAAGGGGCGATAACATACCACTTAGGATAAGTGATGGAGAGTGAGGTGGCTAAAGAAAAAGCTGCCGCAGTGTGTCCTGAAGGGAATGACGGACTATCGGCATCCGGTGCTCCGTATGCATGAATCTTATCCGGATACTTTACAAAAGGCCGGTCACGACCTATTATATGTTTGGCGGCGTATGTAATCCCCACTGCTTCAATCACACTGGTTCCTATGTAAATGGCATCTTTCAGCATCGGCTGGTCTTTCTTTATCAATGCATAGAGTCCCATGGCTGTAGGTATTCCTACGGGTAGAATCACTCCGCTAGCGGACAATCCGCGGCTCAGTCCGTGTACATCCCATCGGTTGATGGTTTTCAACGTGTTGATGTCTATATTCTGTGCGCTGAGGCTGAATGTAAAACAGGCTGTCAGTATAAGAAAAATAATCCTTTGGCGTTTCATTTAGTTTGGCATTTGTTCAGTGCAAAGATAGGAAAATAATGTAATTCGCATTTACCCTTCCCCGAATTAAAGAATAATTTCCATAATTCCTTGTATTATCGCTAGAATTCCTTTACTTTTGCATCGTGAATACACAGCTTTTAACAGAATTTTTAGTTCTAACAATTAAATAATTTAAATTCAATCATTTATGTGGTTAAGTAATTCATCTATTGGAAGGAAAGTGGTGATGAGTGTCACAGGTATCGCCCTTGTCCTGTTTCTGACGTTTCACATGGCGATGAACCTTGTTGCGCTCTTCTCGGAAGATGCTTACAACATGATTTGTGAATTCTTGGGAGCTAACTGGTACGCGTTGGTTGCAACTGCCGGGCTGGCTGCTCTGTTTGTAATTCACATCATCTACGCTTTCTGGTTGACCATGCAGAACCGTGCAGCTCGCGGTAGCGAACGTTATGCAGTGAACGTTAAACCTAAAACTGTGGAATGGGCATCTCAGAACATGCTTGTCCTGGGTATTGTCGTTATTGCCGGATTGGCTCTTCATTTTGCCAATTTCTGGTACAAAATGCAGTTTGCCGAAATTATCGGTGTTCATGATTTGGGCGCATTCGGTCCTACAGACGGTGCCGCTTACATTCGTGAAACATTCGGATGCCCTGTATTTACAGTGCTTTACCTCATTTGGTTAGCAGCTTTGTGGTTCCACCTGACTCACGGATTCTGGAGTGCTTTGCAGACTTTGGGCTGGAGCAATAAAATCTGGTTCGAGCGTTGGAAGACTATTGGTAACATCTACTCTACTTTGGTAGTTCTTGGTTTTGCAGCAGTAGTCGTTATCTACTTCGCTATGACTGGCGCGCCTGATAGGTGGGCTGCCCTTCCTGGTTGGCTTGGTTTCATCAGCCATCCGCTTGCCCTCATCTGTTACGCCGGCGGTAGCCGGCCAGCCTCGCAGAGCAGGATTCCCGTTGAGCACCGCCAGGTGCGAATAAGGGACAGTGAAGAAGGAACACCCGCTCGCGGGTGGGCCTACTTCACCTATCCTGCCCGGCTGACGCCGTTGGATACACCAAGGAAAGTCTACACGAACCCTTTGGCAAAATCCTGTATATCGTGCGAAAAAGGATGGATATACCGAAAAAATCGCTATAATGACCCCGAAGCAGGGTTATGCAGCGGAAAAGCGGGATTAAAAGTCGGGGATTGGTGAACAAAAAGGTGTTTCTCTCTTTAAGAGAAATATCGTTTTGCTAAACAGTTGATATTGAGGTATCATTTTATCGTAAAAGACATTTTTGCTCAACAATTGCTTGACGGAAATCAACAAATTTTAGCATTTTGTAAAAAAGTCGCTATATAATTTGGTGAATTGGAGTTATTTTCATATTTTTGCATCCCGAAGAGTTTCTCTTAAAGAGAGAAACATCTTTTGCATACCTTTTCCGACCGAATTTTTATGTCGTAAAGAGGGGCTTTGCAGGGGGTGGACTCAGAAAGATGAGAATAGATGACTATTGTAGTTGAAACACATAGAAAGTTGCTGATATACAGACCGATACGCATATCGGGATGAACCATGAGTACGTTCTTTTCTCAAAAAACATAAATATTCGAAAAGAGATGCAATAAATTAAGGAGAGGTTATAATGAACAAAGTAAATATAAAAGATAGTCAAAATTTTATTACTTCAAAATATCACATAGAAAAAATAATGAATTGCATAAGTTTAGATGAAAAAGATAACATCTTTGAAATAGGTGCAGGGAAAGGTCATTTTACTGCTGGATTGGTAAAGAGATGTAATTTTGTAACGGCGATAGAAATTGATTCTAAATTATGTGAGGTAACTCGTAATAAGCTCTTAAATTATCCTAACTATCAAATAGTAAATGATGATATACTGAAATTTACATTTCCTAGCCACAATCCATATAAAATATTTGGCAGCATACCTTACAACATAAGCACAAATATAATTCGAAAAATTGTTTTTGAAAGTTCAGCCACAATAAGTTATTTAATAGTGGAATATGGTTTTGCTAAAATGTTATTAGATACAAACAGATCACTAGCATTGCTGTTAATGGCAGAGGTAGATATTTCTATATTAGCAAAAATTCCTAGGTATTATTTCCATCCAAAACCTAAAGTGGATAGCACATTAATTGTATTAAAAAGAAAGCCAGCAAAAATGGCATTTAAAGAGAGAAAAAAATATGAAACTTTTGTAATGAAATGGGTTAACAAAGAGTACGAAAAACTGTTTACAAAAAATCAATTTAATAAAGCTTTAAAACATGCGAGAATATATGATATAAACAATATTAGTTTCGAACAATTTGTATCGCTATTTAATAGTTATAAAATATTTAACGGCTAAAAACAATAGGCCACATGCAACTGTAAATGTTTACGCGGGTACCGACACCGCGGTGGAGGGGAATTGTGTTACAACCAATTAACCAATTCTGATTAGAAAAACTCATCGAGCATCAAATGAAACTGCAATTTATTCATATCAGGATTATCAATACCATATTTTTGAAAAAGCCGTTTCTGTAATGAAGGAGAAAACTCACCGAGGCAGTTCCATAGGATGGCAAGATCCTGGTATCGGTCTGCGATTCCGACTCGTCCAACATCAATACAACCTATTAATTTCCCCTCGTCAAAAATAAGGTTATCAAGTGAGAAATCACCATGAGTGACGACTGAATCCGGTGAGAATGGCAAAAGCTTATGCATTTCTTTCCAGACTTGTTCAACAGGCCAGCCATTACGCTCGTCATCAAAATCACTCGCATCAACCAAACCGTTATTCATTCGTGATTGCGCCTGAGCGAGGCGAAATACGCGATCGCTGTTAAAAGGACAATTACAAACAGGAATCGAATGCAACCGGCGCAGGAACACTGCCAGCGCATCAACAATATTTTCACCTGAATCAGGATATTCTTCTAATACCTGGAATGCTGTTTTCCCGGGGATCGCAGTGGTGAGTAACCATGCATCATCAGGAGTACGGATAAAATGCTTGATGGTCGGAAGAGGCATAAATTCCGTCAGCCAGTTTAGTCTGACCATCTCATCTGTAACATCATTGGCAACGCTACCTTTGCCATGTTTCAGAAACAACTCTGGCGCATCGGGCTTCCCATACAATCGATAGATTGTCGCACCTGATTGCCCGACATTATCGCGAGCCCATTTATACCCATATAAATCAGCATCCATGTTGGAATTTAATCGCGGCCTGGAGCAAGACGTTTCCCGTTGAATATGGCTCATAACACCCCTTGTATTACTGTTTATGTAAGCAGACAGTTTTATTGTTCATGATGATATATTTTTATCTTGTGCAATGTAACATCAGAGATTTTGAGACACAACGTGGCTTTGTTGAATAAATCGAACTTTTGCTGAGTTGAAGGATCAGAGCCTACGTTCCGAATACGGTCAAAAAAAAGGCCATCCGTCAGGATGGCCTTCGCATTAATATGCCGCTTCGAATTCTTTTAGGAAGCGTGTATCGTTTTCAGAGAACATACGGAGGTCTTTCACCTGATATTTCAGGTTTGTGATACGCTCGATACCCATACCGAGTCCATAACCGCTGTATATTTTGCTGTCTATACCATTTGATTCAAGTACGTTCGGGTCTACCATACCGCAACCGAGGATTTCTACCCAGCCGGTGTGTTTACAGAACGGACATCCTTTACCGCCGCAGATATTACAGCTGATATCCATTTCCGCACTTGGTTCAGCAAACGGGAAGTAAGACGGACGCAGACGGATCTTTGTATCAGCACCGAACATTTCTTTGGCAAAGAGCAGCAATACCTGCTTCAAGTCGGTGAATGATACGTTTTTATCTACATACAGCGCTTCTACCTGATGGAAGAAACAGTGTGCGCGATAGCTGATAGCTTCGTTACGATATACACGTCCCGGACAGATGATGCGGATAGGAGGCTGTGAAGTTTCCATCACACGAGTCTGTACAGAAGAAGTATGTGTACGCAATACTACGTCCGGGTGAGCTTCGATAAAGAAAGTGTCCTGCATATCGCGTGCCGGATGATCTTCGGCAAAGTTCAGTGCCGAGAACACGTGCCAGTCATCTTCAATTTCCGGACCTTCGGCAATGCTGAATCCCAGACGGGCAAAGATATCAATGATTTCGTTCTTTACAATGGTGAGCGGGTGGCGTGTACCGAGTTCTACAGGATAAGCCGAACGCGTCAAATCCAGTCCGTCACAATCGTTGTCCTGACTTTCAAACATTTCTTTCAGCGCGTTGATTTTGTCCTGCGCTTTTGTTTTCAGTTCATTCAGTCTCATGCCGACTTCTTTTTTCTGTTCGGCAGCTACATTACGGAAATCTGCCATTAAGTCGTTAATGGCTCCCTTCTTACTTAGGTATTTGATGCGGAGAGCTTCGAGTTCTTCGGCATTGGAGGCGTGTAAGGCTTCCACCTCTTTCAGAAGTTGTTCAATCTTAGCTATCATTTTTTAATATTTTTAGCGGCCCCGTTAAACAAAATTATTTGTAGAGGCTGTTTCGTCCTCACGGACTCATCAGACCGGAAAGCACATCCGGTGACAGCTCAGGCTACTTTGTTTCTTTCGACACTGCAAATATAAGAACATTATTTGAAAGTTCAAGTGAAACTTTAAATTTTAACAATAGATTAACCATTGCAAACAAAACAAAAAAAAGGTAGCCCAATTGTAAAACGAAAGGCCCAGTCTTTCGACTGAGCCTTTCGTTTTATCCTACAGTCGCTCGGCGATCGAAGGCTTCGGAAAAAAAAGGCCATCCGTCAGGATGGCCTTCGCATTAATATGCCGCTTCGAATTCTTTTAGGAAGCGTGTATCGTTTTCAGAGAACATACGGAGGTCTTTCACCTGATATTTCAGGTTTGTGATACGCTCGATACCCATACCGAGTCCATAACCGCTGTATATTTTGCTGTCTATACCATTTGATTCAAGTACGTTCGGGTCTACCATACCGCAACCGAGGATTTCTACCCAGCCGGTGTGTTTACAGAACGGACATCCTTTACCGCCGCAGATATTACAGCTGATATCCATTTCCGCACTTGGTTCAGCAAACGGGAAGTAAGACGGACGCAGACGGATCTTTGTATCAGCACCGAACATTTCTTTGGCAAAGAGCAGCAATACCTGCTTCAAGTCGGTGAATGATACGTTTTTATCTACATACAGCGCTTCTACCTGATGGAAGAAACAGTGTGCGCGATAGCTGATAGCTTCGTTACGATATACACGTCCCGGACAGATGATGCGGATAGGAGGCTGTGAAGTTTCCATCACACGAGTCTGTACAGAAGAAGTATGTGTACGCAATACTACGTCCGGGTGAGCTTCGATAAAGAAAGTGTCCTGCATATCGCGTGCCGGATGATCTTCGGCAAAGTTCAGTGCCGAGAACACGTGCCAGTCATCTTCAATTTCCGGACCTTCGGCAATGCTGAATCCCAGACGGGCAAAGATATCAATGATTTCGTTCTTTACAATGGTGAGCGGGTGGCGTGTACCGAGTTCTACAGGATAAGCCGAACGCGTCAAATCCAGTCCGTCACAATCGTTGTCCTGACTTTCAAACATTTCTTTCAGCGCGTTGATTTTGTCCTGCGCTTTTGTTTTCAGTTCATTCAGTCTCATGCCGACTTCTTTTTTCTGTTCGGCAGCTACATTACGGAAATCTGCCATTAAGTCGTTAATGGCTCCCTTCTTACTTAGGTATTTGATGCGGAGAGCTTCGAGTTCTTCGGCATTGGAGGCGTGTAAGGCTTCCACCTCTTTCAGAAGTTGTTCAATCTTAGCTATCATTTTTTAATATTTTTAGCGGCCCCGTTAAACAAAATTATTTGTAGAGGCTGTTTCGTCCTCACGGACTCATCAGACCGGAAAGCACATCCGGTGACAGCTCAGGCTACTTTGTTTCTTTCGACACTGCAAATATAAGAACATTATTTGAAAGTTCAAGTGAAACTTTAAATTTTAACAATAGATTAACCATTGCAAACAAAACAAAAAAAAGGTAGCCCAATTGTAAAACGAAAGGCCCAGTCTTTCGACTGAGCCTTTCGTTTTATCCTAGGATCAGCTGTACGTACTCGCAGTTCAACCTGTTGATAGTACGTACTAAGCTCTCATGTTTCACGTACTAAGCTCTCATGTTTAACGTACTAAGCTCTCATGTTTAACGAACTAAACCCTCATGGCTAACGTACTAAGCTCTCATGGCTAACGTACTAAGCTCTCATGTTTCACGTACTAAGCTCTCATGTTTGAACAATAAAATTAATATAAATCAGCAACTTAAATAGCCTCTAAGGTTTTAAGTTTTATAAGAAAAAAAAGAATATATAAGGCTTTTAAAGCTTTTAAGGTTTAACGGTTGTGGACAACAAGCCAGGGATGTAACGCACTGAGAAGCCCTTAGAGCCTCTCAAAGCAATTTTGAGTGACACAGGAACACTTAACGGCTGACATGGGGCGGCCGCGTAG
SEQ ID NO:87(用于基因组整合进普通拟杆菌基因组中的编码草酸盐降解途径的质粒)：
GGAGAGCTGTATGTGGGGTGGAAGAAGCTATCGTAAAAATGCTGGAGCAGCTTGATCCTCATTCTACCTATTCTGATCCGGAGGAAGTGAAGAGAATGAACGAGCCGTTGCAGGGAAACTTCGAAGGTATCGGTATACAGTTTAATATGGCGGAGGACACTTTGCTGGTGATACAGCCTGTATCGGGCGGACCGTCCGAAAAAGTCGGTATTCTGGCCGGTGATCGTATCGTCATGGTGGAAGATACGCTGATAGCCGGTGTAAAGATGAGTACCGAGGATATTATGCGTCGCCTCCGTGGTCCTAAAGATTCAAAAGTGAATCTGAAAATACTTCGCCGAGGGGTGAAGGAACTGCTCCCCTTTACGGTGAAAAGGGATAAAATACCTGTTTACAGCTTGGATGCTTCCTATATGATTAAAGATAAGATCGGTTATATTCGTATCAACCGTTTTGCCGCCACTACCCATGAGGAGTTCAAGAAAGCGCTGACCGGGTTGCAGAAGAAAGGAATGAAAGACTTGATTCTTGATTTGCAGGGCAATGGCGGGGGGTATTTGAATGCTGCTATCGATATTGCCAATGAATTTTTGGGTAAAAAGGAACTGATTGTCTATACGGAGGGCCGTCGTAATCCTCGTAGCGAGTTTTTTGCCAAAGGTACGGGCGAGTTTCAGAACGGTCGTCTGATGGTATTGGTTGATGAATTTTCGGCTTCTGCCAGTGAGATTGTGACCGGAGCTGTTCAGGATTGGGACAGAGGCGTTGTGGTAGGCCGTCGCAGTTTTGGTAAAGGCTTGGTGCAGCGTCCCATTGATTTGCCGGACGGTTCGATGATCCGTCTGACGGTGGCCCGCTATTATACGCCTGCCGGACGTTGTATCCAGAAACCTTACGAGAGCATTGAAAAGTATAATGAAGACCTGATAGACCGTTATAATAAAGGAGAAATGATGAGTGAAGACAGCATCCACTTTCCGGATTCACTGAAGTTCAAGACTCATAGGTTGGCCCGTACGGTATATGGTGGCGGAGGGATCATGCCCGATTATTTTGTGCCGATTGATACGACTCTCTATACTAAATACCATCGTCAGTTGCGTGATAAAGGAGCATTGATGAAAGCTCATTTCCATTTTATTGATGCCCATCGTAAAGAATGGCTGGGCAAATACAAGACTTTCAATGAGTTCTACAAACGTTTTGAAGTGACTCCGGATATGTTGACACAACTGGTTGCTACCGGCAAGGAGATGGGAGTGGAATATAATGAAGAAGAGTATCAAAAGGCCCTGCCTCTGCTGAGGTTGCAGATGAAAGCATTGATAGCCCGTGATTTATGGGATATGAATGAATATTATCACGTCATTAATGACGCGAATGAAAGTATCAGGAAAGCGCTGGAACTGTTGGAGCAACCGGACTTTGAGGGGCTGCTGTTGAAAAAGCGATAGTCAAGACTTTATATTAACTGCAGGGGCGCATAGTTGCACTCCTGCAGTTGTCTGAAAAACTTGTAGGATAAAACGAAAGGCTCAGTCGAAAGACTGGGCCTTTCGTTTTACAATTGGGCTACCTTTTTTTTGTTTTGTTTGCAATGGTTAATCTATTGTTAAAATTTAAAGTTTCACTTGAACTTTCAAATAATGTTCTTATATTTGCAGTGTCGAAAGAAACAAAGTAGCCTGGCATCACCTAAAAATTATAAATAAATTAATAAACTTTTATTAAAAAAAAATTACAAAATGAATAATCCACAAACAGGACAATCAACAGGCCTCTTGGGCAATCGTTGGTTCTACTTGGTATTAGCAGTTTTGCTGATGTGTATGATCTCGGGTGTCCAATATTCCTGGACACTGTACGCTAACCCGGTTAAAGACAACCTTGGCGTTTCTTTGGCTGCGGTTCAGACGGCTTTCACACTCTCTCAGGTCATTCAAGCTGGTTCTCAGCCTGGTGGTGGTTACTTCGTTGATAAATTCGGTCCAAGAATTCCATTGATGTTCGGTGGTGCGATGGTTCTCGCTGGCTGGACCTTCATGGGTATGGTTGACAGTGTTCCTGCTCTGTATGCTCTTTATACTCTGGCCGGTGCAGGTGTTGGTATCGTTTACGGTATCGCGATGAACACGGCTAACAGATGGTTCCCGGACAAACGCGGTCTGGCTTCCGGTTTCACCGCTGCCGGTTACGGTCTGGGTGTTCTGCCGTTCCTGCCACTGATCAGCTCCGTTCTGAAAGTTGAAGGTGTTGGCGCAGCATTCATGTACACCGGTTTGATCATGGGTATCCTGATTATCCTGATCGCTTTCGTTATCCGTTTCCCTGGCCAGCAAGGCGCCAAAAAACAAATCGTTGTTACCGACAAGGATTTCAATTCTGGCGAAATGCTGAGAACACCACAATTCTGGGTTCTGTGGACCGCATTCTTTTCCGTTAACTTTGGTGGTTTGCTGCTGGTTGCCAACAGCGTCCCTTACGGTCGCAGCCTCGGTCTTGCCGCAGGTGTGCTGACGATCGGTGTTTCGATCCAGAACCTGTTCAATGGTGGTTGCCGTCCTTTCTGGGGTTTCGTTTCCGATAAAATCGGCCGTTACAAAACCATGTCCGTCGTTTTCGGTATCAATGCTGTTGTTCTCGCACTTTTCCCGACGATTGCTGCCTTGGGCGATGTAGCCTTTATCGCCATGTTGGCAATCGCATTCTTCACATGGGGTGGTAGCTACGCTCTGTTCCCATCGACCAACAGCGATATTTTCGGTACGGCATACTCTGCCAGAAACTATGGTTTCTTCTGGGCTGCAAAAGCAACTGCCTCGATCTTCGGTGGTGGTCTGGGTGCTGCAATTGCAACCAACTTCGGATGGAATACCGCTTTCCTGATTACTGCGATTACTTCTTTCATCGCATTTGCTCTGGCTACCTTCGTTATTCCAAGAATGGGCCGTCCAGTCAAGAAAATGGTCAAATTGTCTCCAGAAGAAAAAGCTGTACATTAAGGTTCCTAACCTGCCTGGCATCTTAATCAATTTTAAACTCACCAAATCAAAATATGAAAAAAAAAAATAATAAAATGTCAACTCCACTTCAAGGAATTAAAGTTCTCGATTTCACCGGTGTGCAATCTGGCCCATCTTGTACTCAAATGCTGGCCTGGTTTGGCGCTGACGTTATTAAAATTGAACGTCCCGGCGTTGGTGACGTAACGCGTCACCAGCTGCGAGATATTCCTGATATCGATGCGCTTTACTTCACCATGCTTAACAGTAACAAACGTTCTATTGAGTTAAATACCAAAACAGCGGAAGGCAAAGAGGTAATGGAAAAGCTGATCCGCGAAGCTGATATCTTAGTCGAGAACTTTCATCCAGGGGCCATTGATCACATGGGCTTCACCTGGGAGCATATTCAAGAAATCAATCCACGTCTGATTTTTGGTTCGATCAAAGGGTTTGATGAGTGTTCGCCTTATGTGAATGTAAAAGCCTATGAAAACGTTGCTCAGGCAGCGGGTGGCGCGGCATCCACTACGGGTTTTTGGGATGGTCCGCCGCTGGTAAGCGCTGCAGCGTTGGGTGACAGCAACACCGGAATGCATTTGCTGATCGGTTTACTTGCTGCTTTGCTGCATCGCGAAAAAACGGGGCGTGGGCAACGAGTCACCATGTCAATGCAGGATGCCGTATTGAACCTTTGCCGCGTGAAATTACGTGACCAGCAGCGTCTAGATAAATTGGGTTATCTGGAAGAATACCCGCAGTATCCGAATGGTACATTTGGTGATGCAGTTCCCCGCGGTGGTAATGCAGGTGGTGGCGGTCAGCCTGGCTGGATCCTGAAATGTAAAGGCTGGGAAACCGATCCTAACGCCTATATTTATTTCACTATTCAGGAGCAAAACTGGGAAAACACCTGTAAAGCCATCGGCAAACCAGAATGGATTACCGATCCGGCATACAGTACAGCCCATGCACGACAGCCACATATTTTCGATATTTTTGCTGAAATCGAAAAATACACTGTCACTATTGATAAACATGAAGCGGTGGCCTATTTGACTCAGTTTGATATTCCTTGTGCACCGGTTTTAAGTATGAAAGAAATTTCACTTGATCCCTCTTTGCGCCAAAGTGGCAGTGTTGTTGAAGTGGAACAACCGTTGCGTGGAAAATATCTGACCGTTGGTTGTCCAATGAAATTCTCTGCCTTTACGCCGGATATTAAAGCTGCGCCGCTATTAGGTGAACATACCGCTGCTGTATTGCAGGAGCTGGGTTATAGCGACGATGAAATTGCTGCAATGAAGCAAAACCACGCCATCTGAGGTTCCTAAACGACCTGGCATCAACTACCTTTATTATTCAACAAACCAATTTAACATATTAAAAAAATAAAAAAATGACTACCACAGCAACGCAGCATTTTGCCCAGTTACTCCAGAAGTACGGAATTGATACTGTCTTCGGCATAGTAGGTATTCCGATCGTGCAGTTGGCCGACACTATGGTCGCGAATGGAATTAAGTTTATACCGTGTCGCAATGAGCAAGCCGCAAGTTACGCGGCAAGCGCCTACGGCTACATCTCGGACAAACCTGGTGTCCTGTTAATCGTGGGAGGGCCTGGCCTTATTCATGCCTTAGCGGGGATCTATAATAGTATGTCGAACCGCTGGCCCTTGTTGGTCATCGCCGGATCCAGCAGCCAGTCAGACATCCACAAGGGAGGTTTCCAGGAACTTGACCAGGTGTCCTTGCTAAGTCCGTTCCTAAAATTCACTGGTAAACTGACGCCGGATAACATCGATATGATAACCCAAAAAGCACTCAACTATTGCATTCAGGGAACCGCCGGAGTGAGCTATATCGATGTCCCCGCTGACTTCATCGAATACGAGAAGCCGCTTGAGGGCAATGATAGAACTGGTAACGAGCTGCCAATGATCCTGACGCCCAATATCTGCGGTCCAGACCCCAGTAAAATTAAGAAGGTGGTGCAATTGATACTCCAGCACAAGAACAAGAACATATTAATAGTGATAGGTAAAGGAGCAGTGAAGAATAGCCATGAGATTAGACGCTTGGTTAACACCTTTAACTTGCCGTTCCTTCCGACTCCCATGGCGAAAGGCATAGTGCCCGATAGCAGCCCTTTAAATGTGAGCAGCGCCCGGAGCCAGGCTCTTAAGATTGCTGACATCGTGTTAGTATTGGGAGCTCGCCTGAATTGGATCCTTCACTTCGGAACGAGTCCAAAGTGGAATAGCGAGAGTATTTTTATACAATTTGACAGTAACCCGGAAACGCTCGGAGACAATAACGTTAGTCCCGGAGCAGATTTAAGTATCTGGGGGGACATCGGACTATCGGTTACTGCACTGGTAGAAGAGCTCACGAGACAGGACAGTTGTTGGAAATACTCGGGAGTCAAACAGGAGATCAGGGAAAAGATACAGTTAAACCAGACCAGATTGCTCCGTAAGGAAAAAACGCGTGGAGCTCAGCTTAACTATAATCAAGTGTACGGCACCCTTCGTCCGTTAATTGACGACTATCGCACGATCTTGGTCACAGAAGGTGCCAACACAATGGACATCGCTAGAATAAGTTTTCCGACTGATGCACCTCGCAGAAGACTTGATGCTGGTACAAATGCTACAATGGGTATAGGTTTAGGTTACGCATTAGCGTGTAAAGCCAGCCACCCTGAGTTAGACGTTGTTTTAATCCAAGGCGACAGTGCCTTCGGTTTTAGCGCTATGGAGATCGAGACTGCCGTCCGTTGCCAATTAGCCCTTGTGATTGTCGTAATGAATAACTCTGGGATATATCACGGTGAGAAAGACATCGAAGGAGATCTGCCCCCGACCGCTTTGTCAAAAAATTGTAGATATGACTTGGTCGGTAAGGGTTTAGGAGCTAACGACTTCTTCGTCAATACCATCTCAGAGTTATCTCGCTGTTTTCAGCAGGCAGTTCAATTGAGCAGAACCAAGCGCGAAACCTCCGTCATTAATGTCATAATCGAACCGGGAGAGCAGAAACAGATCGCGTTCGCATGGCAAAACAAGCCTAGACTGTAAGGTTCCTAATTCCCCTGGCATCAACTACCTAAATTACTTATCAAACCACTTAGTAGTAAAAAAAATATAACAAAATGACAAATAATGAAAGCAAAGGGCCGTTTGAAGGCTTATTAGTTATCGATATGACACATGTCCTTAATGGACCTTTCGGAACTCAACTTCTTTGTAATATGGGCGCAAGGGTAATTAAAGTTGAGCCGCCGGGTCATGGTGATGATACCCGCACATTTGGTCCCTATGTGGATGGACAGTCACTCTATTACAGTTTTATTAATCATGGCAAAGAGAGTGTGGTTCTTGATTTAAAGAATGATCACGATAAAAGTATATTTATAAATATGCTCAAACAAGCTGATGTATTAGCTGAGAATTTTCGCCCAGGTACAATGGAAAAACTGGGGTTTTCATGGGAAACGCTTCAAGAAATCAACCCGCGCCTCATATATGCTTCATCGTCAGGTTTCGGACATACCGGTCCGCTAAAAGATGCTCCTGCCTACGATACCATCATTCAGGCAATGAGCGGGATAATGATGGAAACAGGATATCCTGATGCTCCGCCAGTGCGCGTTGGTACATCTCTTGCGGATCTATGCGGCGGTGTCTATTTATTCAGCGGAATAGTGAGTGCACTTTATGGCCGCGAAAAGAGCCAGAGAGGGGCGCATGTCGATATAGCGATGTTTGATGCCACGCTGAGTTTTCTGGAGCATGGTCTGATGGCATATATCGCAACTGGGAAGTCACCACAACGTCTGGGAAATCGCCATCCCTACATGGCACCTTTTGATGTTTTCAATACTCAGGATAAGCCGATTACGATTTGTTGTGGTAATGACAAGCTTTTTTCTGCGTTATGCCAGGCACTGGAGCTTACGGAACTGGTTAATGATCCCCGATTTAGCAGCAATATTTTACGCGTACAAAACCAGGCTATTCTTAAACAATATATTGAGCGGACGTTAAAAACGCAGGCAGCTGAAGTTTGGTTAGCCAGAATACATGAAGTTGGTGTACCCGTCGCGCCGTTATTAAGTGTGGCTGAGGCCATTAAATTGCCACAAACTCAGGCGAGAAATATGTTGATTGAAGCCGGGGGAATAATGATGCCGGGTAATCCGATAAAAATCAGCGGCTGCGCGGACCCGCATGTTATGCCGGGAGCGGCAACGCTCGACCAGCATGGGGAACAAATTCGCCAGGAGTTCTCATCATAAGGTTCCTAAAATGCCTGGCATCTTTTCCATAACAAAAATTAAACCTCCTCCATATGAAAAATAAAAATTATAAAATGACAAGTGCCGCTACTGTTACTGCTTCGTTCAACGATACTTTTAGCGTATCCGATAATGTCGCCGTTATTGTTCCTGAAACGGACACTCAGGTGACCTACAGGGATCTATCCCACATGGTGGGTCACTTCCAGACCATGTTCACAAATCCTAATTCTCCATTGTACGGAGCTGTTTTCAGACAAGATACAGTGGCGATATCCATGCGTAATGGGCTGGAATTTATCGTCGCTTTCCTCGGTGCTACTATGGACGCTAAAATTGGCGCGCCCTTGAATCCCAATTATAAGGAAAAGGAGTTCAATTTTTATTTGAATGACCTGAAATCTAAGGCGATTTGCGTCCCAAAGGGTACCACAAAGTTACAGAGTTCTGAAATTCTAAAATCTGCCTCCACGTTTGGATGTTTTATCGTAGAGCTGGCCTTCGATGCGACCAGGTTTAGGGTAGAGTATGATATATACTCTCCAGAGGACAACTACAAAAGGGTTATTTACCGGTCTTTGAACAACGCCAAATTTGTCAACACAAATCCCGTTAAATTCCCTGGGTTTGCCCGTTCCAGTGACGTTGCCCTGATTTTGCATACCAGTGGTACCACCTCCACTCCAAAAACGGTGCCTTTGTTACATTTGAACATTGTGAGAAGCACGTTGAACATTGCTAACACTTACAAGCTAACGCCCTTGGACAGATCTTATGTCGTGATGCCTCTTTTCCACGTCCATGGGTTAATTGGTGTTTTACTTTCCACTTTTAGAACTCAGGGTTCTGTTGTGGTTCCCGATGGATTCCATCCAAAGTTATTCTGGGACCAATTTGTTAAGTACAACTGTAATTGGTTCAGTTGCGTTCCCACAATAAGCATGATTATGCTGAACATGCCCAAACCAAACCCTTTCCCACACATTAGATTCATCAGATCGTGTTCTTCTGCTTTGGCTCCAGCAACGTTCCATAAGCTGGAGAAGGAATTCAATGCACCTGTCTTGGAGGCCTATGCGATGACCGAAGCATCACATCAAATGACCTCAAACAATCTGCCTCCAGGAAAGAGAAAGCCTGGTACTGTGGGCCAGCCACAAGGAGTCACCGTCGTCATTCTAGATGACAATGACAATGTCTTGCCCCCGGGCAAAGTCGGCGAAGTTTCCATCAGAGGCGAAAACGTCACTTTGGGGTATGCTAATAATCCAAAAGCTAACAAGGAGAACTTCACCAAGAGAGAGAACTATTTCAGAACCGGTGACCAAGGTTATTTCGACCCTGAGGGGTTTTTGGTCCTTACAGGCAGAATCAAAGAGCTTATCAACAGGGGTGGTGAAAAGATTTCACCCATTGAGCTCGACGGCATTATGCTATCGCATCCAAAGATCGATGAAGCCGTTGCATTTGGTGTTCCCGACGATATGTACGGCCAAGTAGTTCAAGCCGCCATTGTTTTGAAGAAGGGAGAAAAAATGACCTACGAAGAACTGGTGAACTTCTTAAAGAAGCACCTAGCCTCTTTCAAAATTCCAACCAAGGTGTACTTTGTTGATAAGCTACCAAAAACCGCTACAGGTAAAATCCAGAGAAGAGTTATCGCAGAAACTTTTGCTAAGAGCAGCAGAAATAAGAGTAAGTTGTAGGGTTCCTAAAAACCCTGGCATCTAAACTAAAATACAATAACCAAAAATCATTACAAATGAAAAAAAATAATAAAATGGATAGCGATACTCTCTCAGGATTATTGGAAAACGTCGCCAAAAAATTCCCCGATCGCCGAGCTCTCTCCGTTTCTGGAAAATTCAATCTCACTCACGCGCGTCTTCACGATCTAATCGAACGCGCCGCTTCACGCCTTGTCTCCGACGCTGGAATCAAACCCGGCGATGTCGTTGCTCTCACCTTCCCTAACACCGTCGAGTTTGTTATAATGTTTTTAGCGGTGATAAGAGCTAGAGCCACGGCGGCGCCGTTGAACGCAGCGTACACGGCGGAGGAATTTGAGTTTTACCTCTCCGATTCAGATTCAAAGCTATTGTTAACCTCTAAAGAAGGAAACGCACCGGCTCAAGAAGCAGCTTCAAAGCTGAAAATCTCTCACGTCACCGCTACGCTGCTTGACGCTGGCTCGGACCTTGTACTATCCGTTGCGGATTCAGATTCCGTCGTTGACTCAGCGACGGAACTCGTTAATCACCCGGACGACGGTGCTCTCTTCCTCCACACTTCTGGCACTACGAGCCGTCCAAAGGGTGTACCGCTTACGCAGCTCAATCTAGCTTCATCCGTCAAGAACATTAAAGCTGTGTACAAGCTTACTGAGTCTGATTCTACGGTGATTGTTCTCCCTCTGTTCCATGTTCATGGATTGTTAGCTGGGTTGCTTAGCTCGCTTGGAGCTGGTGCTGCTGTAACTCTTCCAGCTGCTGGTAGATTCTCAGCAACAACATTTTGGCCAGATATGAAGAAGTATAACGCTACATGGTATACTGCTGTGCCGACCATTCATCAGATCATATTGGACCGCCACGCGAGCCACCCTGAAACGGAATATCCTAAACTCCGGTTCATCAGGAGTTGCAGTGCTTCTTTGGCTCCGGTGATATTGTCCAGGCTTGAGGAAGCGTTTGGAGCACCGGTGCTCGAGGCCTATGCAATGACAGAGGCAACACATTTGATGAGCTCAAACCCCTTACCAGAGGAAGGTCCACACAAGCCTGGGTCTGTTGGGAAACCGGTAGGTCAAGAAATGGCGATCCTTAATGAGAAAGGCGAGATCCAAGAGCCAAATAACAAAGGAGAGGTTTGTATAAGAGGTCCAAATGTGACCAAGGGTTACAAGAATAACCCAGAAGCCAACAAGGCAGGTTTCGAGTTTGGGTGGTTCCACACTGGTGATATCGGTTACTTTGATACCGATGGGTATTTGCATCTGGTGGGTCGGATCAAAGAGCTTATTAACCGTGGAGGTGAGAAGATATCTCCAATTGAAGTGGATGCAGTACTCTTAACGCATCCTGACGTTTCTCAGGGTGTTGCATTCGGTGTTCCTGATGAGAAATATGGGGAAGAGATTAACTGTGCGGTGATTCCAAGAGAAGGAACTACTGTAACCGAAGAGGACATTAAAGCGTTTTGTAAGAAGAATTTGGCAGCTTTCAAGGTGCCAAAGAGAGTGTTCATCACTGATAACCTCCCCAAAACTGCCTCTGGTAAGATTCAGCGCCGTATCGTCGCACAACATTTCCTTGAGAAGCCCTGAGGTTCCTAGCTGATTAGAAGGCCATCCTGACGGATGGCCTTTTTTTTGACTCTGTTGTGTCTTCTTTTAGGTGTGGAATAATTTTCCAACCATCACTTTATCTGTCTCCCGAACAGCACCTTATTCAGCCATCGGTTCACATACACGTTCACGAAAGCACATCCTACTCCGATAGCGGCTCCTGCCACTACATCACTGGGATAGTGCACCCCTTGGTTCATCCGGGCAAACCCTACTCCGCAAGCCCATACGGCTGAAGGGGCGATAACATACCACTTAGGATAAGTGATGGAGAGTGAGGTGGCTAAAGAAAAAGCTGCCGCAGTGTGTCCTGAAGGGAATGACGGACTATCGGCATCCGGTGCTCCGTATGCATGAATCTTATCCGGATACTTTACAAAAGGCCGGTCACGACCTATTATATGTTTGGCGGCGTATGTAATCCCCACTGCTTCAATCACACTGGTTCCTATGTAAATGGCATCTTTCAGCATCGGCTGGTCTTTCTTTATCAATGCATAGAGTCCCATGGCTGTAGGTATTCCTACGGGTAGAATCACTCCGCTAGCGGACAATCCGCGGCTCAGTCCGTGTACATCCCATCGGTTGATGGTTTTCAACGTGTTGATGTCTATATTCTGTGCGCTGAGGCTGAATGTAAAACAGGCTGTCAGTATAAGAAAAATAATCCTTTGGCGTTTCATTTAGTTTGGCATTTGTTCAGTGCAAAGATAGGAAAATAATGTAATTCGCATTTACCCTTCCCCGAATTAAAGAATAATTTCCATAATTCCTTGTATTATCGCTAGAATTCCTTTACTTTTGCATCGTGAATACACAGCTTTTAACAGAATTTTTAGTTCTAACAATTAAATAATTTAAATTCAATCATTTATGTGGTTAAGTAATTCATCTATTGGAAGGAAAGTGGTGATGAGTGTCACAGGTATCGCCCTTGTCCTGTTTCTGACGTTTCACATGGCGATGAACCTTGTTGCGCTCTTCTCGGAAGATGCTTACAACATGATTTGTGAATTCTTGGGAGCTAACTGGTACGCGTTGGTTGCAACTGCCGGGCTGGCTGCTCTGTTTGTAATTCACATCATCTACGCTTTCTGGTTGACCATGCAGAACCGTGCAGCTCGCGGTAGCGAACGTTATGCAGTGAACGTTAAACCTAAAACTGTGGAATGGGCATCTCAGAACATGCTTGTCCTGGGTATTGTCGTTATTGCCGGATTGGCTCTTCATTTTGCCAATTTCTGGTACAAAATGCAGTTTGCCGAAATTATCGGTGTTCATGATTTGGGCGCATTCGGTCCTACAGACGGTGCCGCTTACATTCGTGAAACATTCGGATGCCCTGTATTTACAGTGCTTTACCTCATTTGGTTAGCAGCTTTGTGGTTCCACCTGACTCACGGATTCTGGAGTGCTTTGCAGACTTTGGGCTGGAGCAATAAAATCTGGTTCGAGCGTTGGAAGACTATTGGTAACATCTACTCTACTTTGGTAGTTCTTGGTTTTGCAGCAGTAGTCGTTATCTACTTCGCTATGACTGGCGCGCCTGATAGGTGGGCTGCCCTTCCTGGTTGGCTTGGTTTCATCAGCCATCCGCTTGCCCTCATCTGTTACGCCGGCGGTAGCCGGCCAGCCTCGCAGAGCAGGATTCCCGTTGAGCACCGCCAGGTGCGAATAAGGGACAGTGAAGAAGGAACACCCGCTCGCGGGTGGGCCTACTTCACCTATCCTGCCCGGCTGACGCCGTTGGATACACCAAGGAAAGTCTACACGAACCCTTTGGCAAAATCCTGTATATCGTGCGAAAAAGGATGGATATACCGAAAAAATCGCTATAATGACCCCGAAGCAGGGTTATGCAGCGGAAAAGCGGGATTAAAAGTCGGGGATTGGTGAACAAAAAGGTGTTTCTCTCTTTAAGAGAAATATCGTTTTGCTAAACAGTTGATATTGAGGTATCATTTTATCGTAAAAGACATTTTTGCTCAACAATTGCTTGACGGAAATCAACAAATTTTAGCATTTTGTAAAAAAGTCGCTATATAATTTGGTGAATTGGAGTTATTTTCATATTTTTGCATCCCGAAGAGTTTCTCTTAAAGAGAGAAACATCTTTTGCATACCTTTTCCGACCGAATTTTTATGTCGTAAAGAGGGGCTTTGCAGGGGGTGGACTCAGAAAGATGAGAATAGATGACTATTGTAGTTGAAACACATAGAAAGTTGCTGATATACAGACCGATACGCATATCGGGATGAACCATGAGTACGTTCTTTTCTCAAAAAACATAAATATTCGAAAAGAGATGCAATAAATTAAGGAGAGGTTATAATGAACAAAGTAAATATAAAAGATAGTCAAAATTTTATTACTTCAAAATATCACATAGAAAAAATAATGAATTGCATAAGTTTAGATGAAAAAGATAACATCTTTGAAATAGGTGCAGGGAAAGGTCATTTTACTGCTGGATTGGTAAAGAGATGTAATTTTGTAACGGCGATAGAAATTGATTCTAAATTATGTGAGGTAACTCGTAATAAGCTCTTAAATTATCCTAACTATCAAATAGTAAATGATGATATACTGAAATTTACATTTCCTAGCCACAATCCATATAAAATATTTGGCAGCATACCTTACAACATAAGCACAAATATAATTCGAAAAATTGTTTTTGAAAGTTCAGCCACAATAAGTTATTTAATAGTGGAATATGGTTTTGCTAAAATGTTATTAGATACAAACAGATCACTAGCATTGCTGTTAATGGCAGAGGTAGATATTTCTATATTAGCAAAAATTCCTAGGTATTATTTCCATCCAAAACCTAAAGTGGATAGCACATTAATTGTATTAAAAAGAAAGCCAGCAAAAATGGCATTTAAAGAGAGAAAAAAATATGAAACTTTTGTAATGAAATGGGTTAACAAAGAGTACGAAAAACTGTTTACAAAAAATCAATTTAATAAAGCTTTAAAACATGCGAGAATATATGATATAAACAATATTAGTTTCGAACAATTTGTATCGCTATTTAATAGTTATAAAATATTTAACGGCTAAAAACAATAGGCCACATGCAACTGTAAATGTTTACGCGGGTACCGACACCGCGGTGGAGGGGAATTGTGTTACAACCAATTAACCAATTCTGATTAGAAAAACTCATCGAGCATCAAATGAAACTGCAATTTATTCATATCAGGATTATCAATACCATATTTTTGAAAAAGCCGTTTCTGTAATGAAGGAGAAAACTCACCGAGGCAGTTCCATAGGATGGCAAGATCCTGGTATCGGTCTGCGATTCCGACTCGTCCAACATCAATACAACCTATTAATTTCCCCTCGTCAAAAATAAGGTTATCAAGTGAGAAATCACCATGAGTGACGACTGAATCCGGTGAGAATGGCAAAAGCTTATGCATTTCTTTCCAGACTTGTTCAACAGGCCAGCCATTACGCTCGTCATCAAAATCACTCGCATCAACCAAACCGTTATTCATTCGTGATTGCGCCTGAGCGAGGCGAAATACGCGATCGCTGTTAAAAGGACAATTACAAACAGGAATCGAATGCAACCGGCGCAGGAACACTGCCAGCGCATCAACAATATTTTCACCTGAATCAGGATATTCTTCTAATACCTGGAATGCTGTTTTCCCGGGGATCGCAGTGGTGAGTAACCATGCATCATCAGGAGTACGGATAAAATGCTTGATGGTCGGAAGAGGCATAAATTCCGTCAGCCAGTTTAGTCTGACCATCTCATCTGTAACATCATTGGCAACGCTACCTTTGCCATGTTTCAGAAACAACTCTGGCGCATCGGGCTTCCCATACAATCGATAGATTGTCGCACCTGATTGCCCGACATTATCGCGAGCCCATTTATACCCATATAAATCAGCATCCATGTTGGAATTTAATCGCGGCCTGGAGCAAGACGTTTCCCGTTGAATATGGCTCATAACACCCCTTGTATTACTGTTTATGTAAGCAGACAGTTTTATTGTTCATGATGATATATTTTTATCTTGTGCAATGTAACATCAGAGATTTTGAGACACAACGTGGCTTTGTTGAATAAATCGAACTTTTGCTGAGTTGAAGGATCAGAGCCTACGTTCCGAATACGGTCAAAAAAAAGGCCATCCGTCAGGATGGCCTTCGCATTAATATGCCGCTTCGAATTCTTTTAGGAAGCGTGTATCGTTTTCAGAGAACATACGGAGGTCTTTCACCTGATATTTCAGGTTTGTGATACGCTCGATACCCATACCGAGTCCATAACCGCTGTATATTTTGCTGTCTATACCATTTGATTCAAGTACGTTCGGGTCTACCATACCGCAACCGAGGATTTCTACCCAGCCGGTGTGTTTACAGAACGGACATCCTTTACCGCCGCAGATATTACAGCTGATATCCATTTCCGCACTTGGTTCAGCAAACGGGAAGTAAGACGGACGCAGACGGATCTTTGTATCAGCACCGAACATTTCTTTGGCAAAGAGCAGCAATACCTGCTTCAAGTCGGTGAATGATACGTTTTTATCTACATACAGCGCTTCTACCTGATGGAAGAAACAGTGTGCGCGATAGCTGATAGCTTCGTTACGATATACACGTCCCGGACAGATGATGCGGATAGGAGGCTGTGAAGTTTCCATCACACGAGTCTGTACAGAAGAAGTATGTGTACGCAATACTACGTCCGGGTGAGCTTCGATAAAGAAAGTGTCCTGCATATCGCGTGCCGGATGATCTTCGGCAAAGTTCAGTGCCGAGAACACGTGCCAGTCATCTTCAATTTCCGGACCTTCGGCAATGCTGAATCCCAGACGGGCAAAGATATCAATGATTTCGTTCTTTACAATGGTGAGCGGGTGGCGTGTACCGAGTTCTACAGGATAAGCCGAACGCGTCAAATCCAGTCCGTCACAATCGTTGTCCTGACTTTCAAACATTTCTTTCAGCGCGTTGATTTTGTCCTGCGCTTTTGTTTTCAGTTCATTCAGTCTCATGCCGACTTCTTTTTTCTGTTCGGCAGCTACATTACGGAAATCTGCCATTAAGTCGTTAATGGCTCCCTTCTTACTTAGGTATTTGATGCGGAGAGCTTCGAGTTCTTCGGCATTGGAGGCGTGTAAGGCTTCCACCTCTTTCAGAAGTTGTTCAATCTTAGCTATCATTTTTTAATATTTTTAGCGGCCCCGTTAAACAAAATTATTTGTAGAGGCTGTTTCGTCCTCACGGACTCATCAGACCGGAAAGCACATCCGGTGACAGCTCAGGCTACTTTGTTTCTTTCGACACTGCAAATATAAGAACATTATTTGAAAGTTCAAGTGAAACTTTAAATTTTAACAATAGATTAACCATTGCAAACAAAACAAAAAAAAGGTAGCCCAATTGTAAAACGAAAGGCCCAGTCTTTCGACTGAGCCTTTCGTTTTATCCTACAGTCGCTCGGCGATCGAAGGCTTCGGAAAAAAAAGGCCATCCGTCAGGATGGCCTTCGCATTAATATGCCGCTTCGAATTCTTTTAGGAAGCGTGTATCGTTTTCAGAGAACATACGGAGGTCTTTCACCTGATATTTCAGGTTTGTGATACGCTCGATACCCATACCGAGTCCATAACCGCTGTATATTTTGCTGTCTATACCATTTGATTCAAGTACGTTCGGGTCTACCATACCGCAACCGAGGATTTCTACCCAGCCGGTGTGTTTACAGAACGGACATCCTTTACCGCCGCAGATATTACAGCTGATATCCATTTCCGCACTTGGTTCAGCAAACGGGAAGTAAGACGGACGCAGACGGATCTTTGTATCAGCACCGAACATTTCTTTGGCAAAGAGCAGCAATACCTGCTTCAAGTCGGTGAATGATACGTTTTTATCTACATACAGCGCTTCTACCTGATGGAAGAAACAGTGTGCGCGATAGCTGATAGCTTCGTTACGATATACACGTCCCGGACAGATGATGCGGATAGGAGGCTGTGAAGTTTCCATCACACGAGTCTGTACAGAAGAAGTATGTGTACGCAATACTACGTCCGGGTGAGCTTCGATAAAGAAAGTGTCCTGCATATCGCGTGCCGGATGATCTTCGGCAAAGTTCAGTGCCGAGAACACGTGCCAGTCATCTTCAATTTCCGGACCTTCGGCAATGCTGAATCCCAGACGGGCAAAGATATCAATGATTTCGTTCTTTACAATGGTGAGCGGGTGGCGTGTACCGAGTTCTACAGGATAAGCCGAACGCGTCAAATCCAGTCCGTCACAATCGTTGTCCTGACTTTCAAACATTTCTTTCAGCGCGTTGATTTTGTCCTGCGCTTTTGTTTTCAGTTCATTCAGTCTCATGCCGACTTCTTTTTTCTGTTCGGCAGCTACATTACGGAAATCTGCCATTAAGTCGTTAATGGCTCCCTTCTTACTTAGGTATTTGATGCGGAGAGCTTCGAGTTCTTCGGCATTGGAGGCGTGTAAGGCTTCCACCTCTTTCAGAAGTTGTTCAATCTTAGCTATCATTTTTTAATATTTTTAGCGGCCCCGTTAAACAAAATTATTTGTAGAGGCTGTTTCGTCCTCACGGACTCATCAGACCGGAAAGCACATCCGGTGACAGCTCAGGCTACTTTGTTTCTTTCGACACTGCAAATATAAGAACATTATTTGAAAGTTCAAGTGAAACTTTAAATTTTAACAATAGATTAACCATTGCAAACAAAACAAAAAAAAGGTAGCCCAATTGTAAAACGAAAGGCCCAGTCTTTCGACTGAGCCTTTCGTTTTATCCTAGGATCAGCTGTACGTACTCGCAGTTCAACCTGTTGATAGTACGTACTAAGCTCTCATGTTTCACGTACTAAGCTCTCATGTTTAACGTACTAAGCTCTCATGTTTAACGAACTAAACCCTCATGGCTAACGTACTAAGCTCTCATGGCTAACGTACTAAGCTCTCATGTTTCACGTACTAAGCTCTCATGTTTGAACAATAAAATTAATATAAATCAGCAACTTAAATAGCCTCTAAGGTTTTAAGTTTTATAAGAAAAAAAAGAATATATAAGGCTTTTAAAGCTTTTAAGGTTTAACGGTTGTGGACAACAAGCCAGGGATGTAACGCACTGAGAAGCCCTTAGAGCCTCTCAAAGCAATTTTGAGTGACACAGGAACACTTAACGGCTGACATGGGGCGGCCGCGTAG
SEQ ID NO:88(用于基因组整合进普通拟杆菌基因组中的编码草酸盐降解途径的质粒)：
GGAGAGCTGTATGTGGGGTGGAAGAAGCTATCGTAAAAATGCTGGAGCAGCTTGATCCTCATTCTACCTATTCTGATCCGGAGGAAGTGAAGAGAATGAACGAGCCGTTGCAGGGAAACTTCGAAGGTATCGGTATACAGTTTAATATGGCGGAGGACACTTTGCTGGTGATACAGCCTGTATCGGGCGGACCGTCCGAAAAAGTCGGTATTCTGGCCGGTGATCGTATCGTCATGGTGGAAGATACGCTGATAGCCGGTGTAAAGATGAGTACCGAGGATATTATGCGTCGCCTCCGTGGTCCTAAAGATTCAAAAGTGAATCTGAAAATACTTCGCCGAGGGGTGAAGGAACTGCTCCCCTTTACGGTGAAAAGGGATAAAATACCTGTTTACAGCTTGGATGCTTCCTATATGATTAAAGATAAGATCGGTTATATTCGTATCAACCGTTTTGCCGCCACTACCCATGAGGAGTTCAAGAAAGCGCTGACCGGGTTGCAGAAGAAAGGAATGAAAGACTTGATTCTTGATTTGCAGGGCAATGGCGGGGGGTATTTGAATGCTGCTATCGATATTGCCAATGAATTTTTGGGTAAAAAGGAACTGATTGTCTATACGGAGGGCCGTCGTAATCCTCGTAGCGAGTTTTTTGCCAAAGGTACGGGCGAGTTTCAGAACGGTCGTCTGATGGTATTGGTTGATGAATTTTCGGCTTCTGCCAGTGAGATTGTGACCGGAGCTGTTCAGGATTGGGACAGAGGCGTTGTGGTAGGCCGTCGCAGTTTTGGTAAAGGCTTGGTGCAGCGTCCCATTGATTTGCCGGACGGTTCGATGATCCGTCTGACGGTGGCCCGCTATTATACGCCTGCCGGACGTTGTATCCAGAAACCTTACGAGAGCATTGAAAAGTATAATGAAGACCTGATAGACCGTTATAATAAAGGAGAAATGATGAGTGAAGACAGCATCCACTTTCCGGATTCACTGAAGTTCAAGACTCATAGGTTGGCCCGTACGGTATATGGTGGCGGAGGGATCATGCCCGATTATTTTGTGCCGATTGATACGACTCTCTATACTAAATACCATCGTCAGTTGCGTGATAAAGGAGCATTGATGAAAGCTCATTTCCATTTTATTGATGCCCATCGTAAAGAATGGCTGGGCAAATACAAGACTTTCAATGAGTTCTACAAACGTTTTGAAGTGACTCCGGATATGTTGACACAACTGGTTGCTACCGGCAAGGAGATGGGAGTGGAATATAATGAAGAAGAGTATCAAAAGGCCCTGCCTCTGCTGAGGTTGCAGATGAAAGCATTGATAGCCCGTGATTTATGGGATATGAATGAATATTATCACGTCATTAATGACGCGAATGAAAGTATCAGGAAAGCGCTGGAACTGTTGGAGCAACCGGACTTTGAGGGGCTGCTGTTGAAAAAGCGATAGTCAAGACTTTATATTAACTGCAGGGGCGCATAGTTGCACTCCTGCAGTTGTCTGAAAAACTTGTAGGATAAAACGAAAGGCTCAGTCGAAAGACTGGGCCTTTCGTTTTACAATTGGGCTACCTTTTTTTTGTTTTGTTTGCAATGGTTAATCTATTGTTAAAATTTAAAGTTTCACTTGAACTTTCAAATAATGTTCTTATATTTGCAGTGTCGAAAGAAACAAAGTAGCCTGGCATCATCTACCTTCATTATTAATCATTCCAATTGTATCACTTAAAAATATAAAAAAATGTCAACTCCACTTCAAGGAATTAAAGTTCTCGATTTCACCGGTGTGCAATCTGGCCCATCTTGTACTCAAATGCTGGCCTGGTTTGGCGCTGACGTTATTAAAATTGAACGTCCCGGCGTTGGTGACGTAACGCGTCACCAGCTGCGAGATATTCCTGATATCGATGCGCTTTACTTCACCATGCTTAACAGTAACAAACGTTCTATTGAGTTAAATACCAAAACAGCGGAAGGCAAAGAGGTAATGGAAAAGCTGATCCGCGAAGCTGATATCTTAGTCGAGAACTTTCATCCAGGGGCCATTGATCACATGGGCTTCACCTGGGAGCATATTCAAGAAATCAATCCACGTCTGATTTTTGGTTCGATCAAAGGGTTTGATGAGTGTTCGCCTTATGTGAATGTAAAAGCCTATGAAAACGTTGCTCAGGCAGCGGGTGGCGCGGCATCCACTACGGGTTTTTGGGATGGTCCGCCGCTGGTAAGCGCTGCAGCGTTGGGTGACAGCAACACCGGAATGCATTTGCTGATCGGTTTACTTGCTGCTTTGCTGCATCGCGAAAAAACGGGGCGTGGGCAACGAGTCACCATGTCAATGCAGGATGCCGTATTGAACCTTTGCCGCGTGAAATTACGTGACCAGCAGCGTCTAGATAAATTGGGTTATCTGGAAGAATACCCGCAGTATCCGAATGGTACATTTGGTGATGCAGTTCCCCGCGGTGGTAATGCAGGTGGTGGCGGTCAGCCTGGCTGGATCCTGAAATGTAAAGGCTGGGAAACCGATCCTAACGCCTATATTTATTTCACTATTCAGGAGCAAAACTGGGAAAACACCTGTAAAGCCATCGGCAAACCAGAATGGATTACCGATCCGGCATACAGTACAGCCCATGCACGACAGCCACATATTTTCGATATTTTTGCTGAAATCGAAAAATACACTGTCACTATTGATAAACATGAAGCGGTGGCCTATTTGACTCAGTTTGATATTCCTTGTGCACCGGTTTTAAGTATGAAAGAAATTTCACTTGATCCCTCTTTGCGCCAAAGTGGCAGTGTTGTTGAAGTGGAACAACCGTTGCGTGGAAAATATCTGACCGTTGGTTGTCCAATGAAATTCTCTGCCTTTACGCCGGATATTAAAGCTGCGCCGCTATTAGGTGAACATACCGCTGCTGTATTGCAGGAGCTGGGTTATAGCGACGATGAAATTGCTGCAATGAAGCAAAACCACGCCATCTGAGGTTCCTAACCTGCCTGGCATCAACTACCTAAATTACTTATCAAACCACTTAGTAGTAAAAAAAATATAACAAAATGACAAATAATGAAAGCAAAGGGCCGTTTGAAGGCTTATTAGTTATCGATATGACACATGTCCTTAATGGACCTTTCGGAACTCAACTTCTTTGTAATATGGGCGCAAGGGTAATTAAAGTTGAGCCGCCGGGTCATGGTGATGATACCCGCACATTTGGTCCCTATGTGGATGGACAGTCACTCTATTACAGTTTTATTAATCATGGCAAAGAGAGTGTGGTTCTTGATTTAAAGAATGATCACGATAAAAGTATATTTATAAATATGCTCAAACAAGCTGATGTATTAGCTGAGAATTTTCGCCCAGGTACAATGGAAAAACTGGGGTTTTCATGGGAAACGCTTCAAGAAATCAACCCGCGCCTCATATATGCTTCATCGTCAGGTTTCGGACATACCGGTCCGCTAAAAGATGCTCCTGCCTACGATACCATCATTCAGGCAATGAGCGGGATAATGATGGAAACAGGATATCCTGATGCTCCGCCAGTGCGCGTTGGTACATCTCTTGCGGATCTATGCGGCGGTGTCTATTTATTCAGCGGAATAGTGAGTGCACTTTATGGCCGCGAAAAGAGCCAGAGAGGGGCGCATGTCGATATAGCGATGTTTGATGCCACGCTGAGTTTTCTGGAGCATGGTCTGATGGCATATATCGCAACTGGGAAGTCACCACAACGTCTGGGAAATCGCCATCCCTACATGGCACCTTTTGATGTTTTCAATACTCAGGATAAGCCGATTACGATTTGTTGTGGTAATGACAAGCTTTTTTCTGCGTTATGCCAGGCACTGGAGCTTACGGAACTGGTTAATGATCCCCGATTTAGCAGCAATATTTTACGCGTACAAAACCAGGCTATTCTTAAACAATATATTGAGCGGACGTTAAAAACGCAGGCAGCTGAAGTTTGGTTAGCCAGAATACATGAAGTTGGTGTACCCGTCGCGCCGTTATTAAGTGTGGCTGAGGCCATTAAATTGCCACAAACTCAGGCGAGAAATATGTTGATTGAAGCCGGGGGAATAATGATGCCGGGTAATCCGATAAAAATCAGCGGCTGCGCGGACCCGCATGTTATGCCGGGAGCGGCAACGCTCGACCAGCATGGGGAACAAATTCGCCAGGAGTTCTCATCATAAGGTTCCTAAACGACCTGGCATCATCTACCTTCATTATTAAACAAACCAAATTAATATCATAAAAAAATAAAAAAATGACTACCACAGCAACGCAGCATTTTGCCCAGTTACTCCAGAAGTACGGAATTGATACTGTCTTCGGCATAGTAGGTATTCCGATCGTGCAGTTGGCCGACACTATGGTCGCGAATGGAATTAAGTTTATACCGTGTCGCAATGAGCAAGCCGCAAGTTACGCGGCAAGCGCCTACGGCTACATCTCGGACAAACCTGGTGTCCTGTTAATCGTGGGAGGGCCTGGCCTTATTCATGCCTTAGCGGGGATCTATAATAGTATGTCGAACCGCTGGCCCTTGTTGGTCATCGCCGGATCCAGCAGCCAGTCAGACATCCACAAGGGAGGTTTCCAGGAACTTGACCAGGTGTCCTTGCTAAGTCCGTTCCTAAAATTCACTGGTAAACTGACGCCGGATAACATCGATATGATAACCCAAAAAGCACTCAACTATTGCATTCAGGGAACCGCCGGAGTGAGCTATATCGATGTCCCCGCTGACTTCATCGAATACGAGAAGCCGCTTGAGGGCAATGATAGAACTGGTAACGAGCTGCCAATGATCCTGACGCCCAATATCTGCGGTCCAGACCCCAGTAAAATTAAGAAGGTGGTGCAATTGATACTCCAGCACAAGAACAAGAACATATTAATAGTGATAGGTAAAGGAGCAGTGAAGAATAGCCATGAGATTAGACGCTTGGTTAACACCTTTAACTTGCCGTTCCTTCCGACTCCCATGGCGAAAGGCATAGTGCCCGATAGCAGCCCTTTAAATGTGAGCAGCGCCCGGAGCCAGGCTCTTAAGATTGCTGACATCGTGTTAGTATTGGGAGCTCGCCTGAATTGGATCCTTCACTTCGGAACGAGTCCAAAGTGGAATAGCGAGAGTATTTTTATACAATTTGACAGTAACCCGGAAACGCTCGGAGACAATAACGTTAGTCCCGGAGCAGATTTAAGTATCTGGGGGGACATCGGACTATCGGTTACTGCACTGGTAGAAGAGCTCACGAGACAGGACAGTTGTTGGAAATACTCGGGAGTCAAACAGGAGATCAGGGAAAAGATACAGTTAAACCAGACCAGATTGCTCCGTAAGGAAAAAACGCGTGGAGCTCAGCTTAACTATAATCAAGTGTACGGCACCCTTCGTCCGTTAATTGACGACTATCGCACGATCTTGGTCACAGAAGGTGCCAACACAATGGACATCGCTAGAATAAGTTTTCCGACTGATGCACCTCGCAGAAGACTTGATGCTGGTACAAATGCTACAATGGGTATAGGTTTAGGTTACGCATTAGCGTGTAAAGCCAGCCACCCTGAGTTAGACGTTGTTTTAATCCAAGGCGACAGTGCCTTCGGTTTTAGCGCTATGGAGATCGAGACTGCCGTCCGTTGCCAATTAGCCCTTGTGATTGTCGTAATGAATAACTCTGGGATATATCACGGTGAGAAAGACATCGAAGGAGATCTGCCCCCGACCGCTTTGTCAAAAAATTGTAGATATGACTTGGTCGGTAAGGGTTTAGGAGCTAACGACTTCTTCGTCAATACCATCTCAGAGTTATCTCGCTGTTTTCAGCAGGCAGTTCAATTGAGCAGAACCAAGCGCGAAACCTCCGTCATTAATGTCATAATCGAACCGGGAGAGCAGAAACAGATCGCGTTCGCATGGCAAAACAAGCCTAGACTGTAAGGTTCCTAAGGGTCCTGGCATCCCCAAAAAATTAATTCATTTTCTCTTTTCCCTAACCCTTAAAATATAATAAAATGAGTAACGACGACAATGTAGAGTTGACTGATGGCTTTCATGTTTTGATCGATGCCCTGAAAATGAATGACATCGATACCATGTATGGTGTTGTCGGCATTCCTATCACGAACCTGGCTCGTATGTGGCAAGATGACGGTCAGCGTTTTTACAGCTTCCGTCACGAACAACACGCAGGTTATGCAGCTTCTATCGCCGGTTACATCGAAGGAAAACCTGGCGTTTGCTTGACCGTTTCCGCCCCTGGCTTCCTGAACGGCGTGACTTCCCTGGCTCATGCAACCACCAACTGCTTCCCAATGATCCTGTTGAGCGGTTCCAGTGAACGTGAAATCGTCGATTTGCAACAGGGCGATTACGAAGAAATGGATCAGATGAATGTTGCACGTCCACACTGCAAAGCTTCTTTCCGTATCAACAGCATCAAAGACATTCCAATCGGTATCGCTCGTGCAGTTCGCACCGCTGTATCCGGACGTCCAGGTGGTGTTTACGTTGACTTGCCAGCAAAACTGTTCGGTCAGACCATTTCTGTAGAAGAAGCTAACAAACTGCTCTTCAAACCAATCGATCCAGCTCCGGCACAGATTCCTGCTGAAGATGCTATCGCTCGCGCTGCTGACCTGATCAAGAACGCCAAACGTCCAGTTATCATGCTGGGTAAAGGCGCTGCATACGCACAATGCGACGACGAAATCCGCGCACTGGTTGAAGAAACCGGCATCCCATTCCTGCCAATGGGTATGGCTAAAGGCCTGCTGCCTGACAACCATCCACAATCCGCTGCTGCAACCCGTGCTTTCGCACTGGCACAGTGTGACGTTTGCGTACTGATCGGCGCTCGTCTGAACTGGCTGATGCAGCACGGTAAAGGCAAAACCTGGGGCGACGAACTGAAGAAATACGTTCAGATCGACATCCAGGCTAACGAAATGGACAGCAACCAGCCTATCGCTGCACCAGTTGTTGGTGACATCAAGTCCGCCGTTTCCCTGCTCCGCAAAGCACTGAAAGGCGCTCCAAAAGCTGACGCTGAATGGACCGGCGCTCTGAAAGCCAAAGTTGACGGCAACAAAGCCAAACTGGCTGGCAAGATGACTGCCGAAACCCCATCCGGAATGATGAACTACTCCAATTCCCTGGGCGTTGTTCGTGACTTCATGCTGGCAAATCCGGATATTTCCCTGGTTAACGAAGGCGCTAATGCACTCGACAACACTCGTATGATTGTTGACATGCTGAAACCACGCAAACGTCTTGACTCCGGTACCTGGGGTGTTATGGGTATTGGTATGGGCTACTGCGTTGCTGCAGCTGCTGTTACCGGCAAACCGGTTATCGCTGTTGAAGGCGATAGCGCATTCGGTTTCTCCGGTATGGAACTGGAAACCATCTGCCGTTACAACCTGCCAGTTACCGTTATCATCATGAACAATGGTGGTATCTATAAAGGTAACGAAGCAGATCCACAACCAGGCGTTATCTCCTGTACCCGTCTGACCCGTGGTCGTTACGACATGATGATGGAAGCATTTGGCGGTAAAGGTTATGTTGCCAATACTCCAGCAGAACTGAAAGCTGCTCTGGAAGAAGCTGTTGCTTCCGGCAAACCATGCCTGATCAACGCGATGATCGATCCAGACGCTGGTGTCGAATCTGGCCGTATCAAGAGCCTGAACGTTGTAAGTAAAGTTGGCAAGAAATAAGGTTCCTAATTCCCCTGGCATCTAAACTAAAATACAATAACCAAAAATCATACTACATGTATAAAATTAAAAAAATGGATAGCGATACTCTCTCAGGATTATTGGAAAACGTCGCCAAAAAATTCCCCGATCGCCGAGCTCTCTCCGTTTCTGGAAAATTCAATCTCACTCACGCGCGTCTTCACGATCTAATCGAACGCGCCGCTTCACGCCTTGTCTCCGACGCTGGAATCAAACCCGGCGATGTCGTTGCTCTCACCTTCCCTAACACCGTCGAGTTTGTTATAATGTTTTTAGCGGTGATAAGAGCTAGAGCCACGGCGGCGCCGTTGAACGCAGCGTACACGGCGGAGGAATTTGAGTTTTACCTCTCCGATTCAGATTCAAAGCTATTGTTAACCTCTAAAGAAGGAAACGCACCGGCTCAAGAAGCAGCTTCAAAGCTGAAAATCTCTCACGTCACCGCTACGCTGCTTGACGCTGGCTCGGACCTTGTACTATCCGTTGCGGATTCAGATTCCGTCGTTGACTCAGCGACGGAACTCGTTAATCACCCGGACGACGGTGCTCTCTTCCTCCACACTTCTGGCACTACGAGCCGTCCAAAGGGTGTACCGCTTACGCAGCTCAATCTAGCTTCATCCGTCAAGAACATTAAAGCTGTGTACAAGCTTACTGAGTCTGATTCTACGGTGATTGTTCTCCCTCTGTTCCATGTTCATGGATTGTTAGCTGGGTTGCTTAGCTCGCTTGGAGCTGGTGCTGCTGTAACTCTTCCAGCTGCTGGTAGATTCTCAGCAACAACATTTTGGCCAGATATGAAGAAGTATAACGCTACATGGTATACTGCTGTGCCGACCATTCATCAGATCATATTGGACCGCCACGCGAGCCACCCTGAAACGGAATATCCTAAACTCCGGTTCATCAGGAGTTGCAGTGCTTCTTTGGCTCCGGTGATATTGTCCAGGCTTGAGGAAGCGTTTGGAGCACCGGTGCTCGAGGCCTATGCAATGACAGAGGCAACACATTTGATGAGCTCAAACCCCTTACCAGAGGAAGGTCCACACAAGCCTGGGTCTGTTGGGAAACCGGTAGGTCAAGAAATGGCGATCCTTAATGAGAAAGGCGAGATCCAAGAGCCAAATAACAAAGGAGAGGTTTGTATAAGAGGTCCAAATGTGACCAAGGGTTACAAGAATAACCCAGAAGCCAACAAGGCAGGTTTCGAGTTTGGGTGGTTCCACACTGGTGATATCGGTTACTTTGATACCGATGGGTATTTGCATCTGGTGGGTCGGATCAAAGAGCTTATTAACCGTGGAGGTGAGAAGATATCTCCAATTGAAGTGGATGCAGTACTCTTAACGCATCCTGACGTTTCTCAGGGTGTTGCATTCGGTGTTCCTGATGAGAAATATGGGGAAGAGATTAACTGTGCGGTGATTCCAAGAGAAGGAACTACTGTAACCGAAGAGGACATTAAAGCGTTTTGTAAGAAGAATTTGGCAGCTTTCAAGGTGCCAAAGAGAGTGTTCATCACTGATAACCTCCCCAAAACTGCCTCTGGTAAGATTCAGCGCCGTATCGTCGCACAACATTTCCTTGAGAAGCCCTGAGGTTCCTAAAATGCCTGGCATCAACTACCTCAATTAATAATCAAACCATCTGAACAATGAATAAATTTAAAAAAATGACAAGTGCCGCTACTGTTACTGCTTCGTTCAACGATACTTTTAGCGTATCCGATAATGTCGCCGTTATTGTTCCTGAAACGGACACTCAGGTGACCTACAGGGATCTATCCCACATGGTGGGTCACTTCCAGACCATGTTCACAAATCCTAATTCTCCATTGTACGGAGCTGTTTTCAGACAAGATACAGTGGCGATATCCATGCGTAATGGGCTGGAATTTATCGTCGCTTTCCTCGGTGCTACTATGGACGCTAAAATTGGCGCGCCCTTGAATCCCAATTATAAGGAAAAGGAGTTCAATTTTTATTTGAATGACCTGAAATCTAAGGCGATTTGCGTCCCAAAGGGTACCACAAAGTTACAGAGTTCTGAAATTCTAAAATCTGCCTCCACGTTTGGATGTTTTATCGTAGAGCTGGCCTTCGATGCGACCAGGTTTAGGGTAGAGTATGATATATACTCTCCAGAGGACAACTACAAAAGGGTTATTTACCGGTCTTTGAACAACGCCAAATTTGTCAACACAAATCCCGTTAAATTCCCTGGGTTTGCCCGTTCCAGTGACGTTGCCCTGATTTTGCATACCAGTGGTACCACCTCCACTCCAAAAACGGTGCCTTTGTTACATTTGAACATTGTGAGAAGCACGTTGAACATTGCTAACACTTACAAGCTAACGCCCTTGGACAGATCTTATGTCGTGATGCCTCTTTTCCACGTCCATGGGTTAATTGGTGTTTTACTTTCCACTTTTAGAACTCAGGGTTCTGTTGTGGTTCCCGATGGATTCCATCCAAAGTTATTCTGGGACCAATTTGTTAAGTACAACTGTAATTGGTTCAGTTGCGTTCCCACAATAAGCATGATTATGCTGAACATGCCCAAACCAAACCCTTTCCCACACATTAGATTCATCAGATCGTGTTCTTCTGCTTTGGCTCCAGCAACGTTCCATAAGCTGGAGAAGGAATTCAATGCACCTGTCTTGGAGGCCTATGCGATGACCGAAGCATCACATCAAATGACCTCAAACAATCTGCCTCCAGGAAAGAGAAAGCCTGGTACTGTGGGCCAGCCACAAGGAGTCACCGTCGTCATTCTAGATGACAATGACAATGTCTTGCCCCCGGGCAAAGTCGGCGAAGTTTCCATCAGAGGCGAAAACGTCACTTTGGGGTATGCTAATAATCCAAAAGCTAACAAGGAGAACTTCACCAAGAGAGAGAACTATTTCAGAACCGGTGACCAAGGTTATTTCGACCCTGAGGGGTTTTTGGTCCTTACAGGCAGAATCAAAGAGCTTATCAACAGGGGTGGTGAAAAGATTTCACCCATTGAGCTCGACGGCATTATGCTATCGCATCCAAAGATCGATGAAGCCGTTGCATTTGGTGTTCCCGACGATATGTACGGCCAAGTAGTTCAAGCCGCCATTGTTTTGAAGAAGGGAGAAAAAATGACCTACGAAGAACTGGTGAACTTCTTAAAGAAGCACCTAGCCTCTTTCAAAATTCCAACCAAGGTGTACTTTGTTGATAAGCTACCAAAAACCGCTACAGGTAAAATCCAGAGAAGAGTTATCGCAGAAACTTTTGCTAAGAGCAGCAGAAATAAGAGTAAGTTGTAGGGTTCCTAAAAACCCTGGCATCACCTAAAAATTATAAATAAATTAATAAACTTTTATTAAAAAAAAATTACAAAATGAATAATCCACAAACAGGACAATCAACAGGCCTCTTGGGCAATCGTTGGTTCTACTTGGTATTAGCAGTTTTGCTGATGTGTATGATCTCGGGTGTCCAATATTCCTGGACACTGTACGCTAACCCGGTTAAAGACAACCTTGGCGTTTCTTTGGCTGCGGTTCAGACGGCTTTCACACTCTCTCAGGTCATTCAAGCTGGTTCTCAGCCTGGTGGTGGTTACTTCGTTGATAAATTCGGTCCAAGAATTCCATTGATGTTCGGTGGTGCGATGGTTCTCGCTGGCTGGACCTTCATGGGTATGGTTGACAGTGTTCCTGCTCTGTATGCTCTTTATACTCTGGCCGGTGCAGGTGTTGGTATCGTTTACGGTATCGCGATGAACACGGCTAACAGATGGTTCCCGGACAAACGCGGTCTGGCTTCCGGTTTCACCGCTGCCGGTTACGGTCTGGGTGTTCTGCCGTTCCTGCCACTGATCAGCTCCGTTCTGAAAGTTGAAGGTGTTGGCGCAGCATTCATGTACACCGGTTTGATCATGGGTATCCTGATTATCCTGATCGCTTTCGTTATCCGTTTCCCTGGCCAGCAAGGCGCCAAAAAACAAATCGTTGTTACCGACAAGGATTTCAATTCTGGCGAAATGCTGAGAACACCACAATTCTGGGTTCTGTGGACCGCATTCTTTTCCGTTAACTTTGGTGGTTTGCTGCTGGTTGCCAACAGCGTCCCTTACGGTCGCAGCCTCGGTCTTGCCGCAGGTGTGCTGACGATCGGTGTTTCGATCCAGAACCTGTTCAATGGTGGTTGCCGTCCTTTCTGGGGTTTCGTTTCCGATAAAATCGGCCGTTACAAAACCATGTCCGTCGTTTTCGGTATCAATGCTGTTGTTCTCGCACTTTTCCCGACGATTGCTGCCTTGGGCGATGTAGCCTTTATCGCCATGTTGGCAATCGCATTCTTCACATGGGGTGGTAGCTACGCTCTGTTCCCATCGACCAACAGCGATATTTTCGGTACGGCATACTCTGCCAGAAACTATGGTTTCTTCTGGGCTGCAAAAGCAACTGCCTCGATCTTCGGTGGTGGTCTGGGTGCTGCAATTGCAACCAACTTCGGATGGAATACCGCTTTCCTGATTACTGCGATTACTTCTTTCATCGCATTTGCTCTGGCTACCTTCGTTATTCCAAGAATGGGCCGTCCAGTCAAGAAAATGGTCAAATTGTCTCCAGAAGAAAAAGCTGTACATTAAGGTTCCTAGCTGATTAGAAGGCCATCCTGACGGATGGCCTTTTTTTTGACTCTGTTGTGTCTTCTTTTAGGTGTGGAATAATTTTCCAACCATCACTTTATCTGTCTCCCGAACAGCACCTTATTCAGCCATCGGTTCACATACACGTTCACGAAAGCACATCCTACTCCGATAGCGGCTCCTGCCACTACATCACTGGGATAGTGCACCCCTTGGTTCATCCGGGCAAACCCTACTCCGCAAGCCCATACGGCTGAAGGGGCGATAACATACCACTTAGGATAAGTGATGGAGAGTGAGGTGGCTAAAGAAAAAGCTGCCGCAGTGTGTCCTGAAGGGAATGACGGACTATCGGCATCCGGTGCTCCGTATGCATGAATCTTATCCGGATACTTTACAAAAGGCCGGTCACGACCTATTATATGTTTGGCGGCGTATGTAATCCCCACTGCTTCAATCACACTGGTTCCTATGTAAATGGCATCTTTCAGCATCGGCTGGTCTTTCTTTATCAATGCATAGAGTCCCATGGCTGTAGGTATTCCTACGGGTAGAATCACTCCGCTAGCGGACAATCCGCGGCTCAGTCCGTGTACATCCCATCGGTTGATGGTTTTCAACGTGTTGATGTCTATATTCTGTGCGCTGAGGCTGAATGTAAAACAGGCTGTCAGTATAAGAAAAATAATCCTTTGGCGTTTCATTTAGTTTGGCATTTGTTCAGTGCAAAGATAGGAAAATAATGTAATTCGCATTTACCCTTCCCCGAATTAAAGAATAATTTCCATAATTCCTTGTATTATCGCTAGAATTCCTTTACTTTTGCATCGTGAATACACAGCTTTTAACAGAATTTTTAGTTCTAACAATTAAATAATTTAAATTCAATCATTTATGTGGTTAAGTAATTCATCTATTGGAAGGAAAGTGGTGATGAGTGTCACAGGTATCGCCCTTGTCCTGTTTCTGACGTTTCACATGGCGATGAACCTTGTTGCGCTCTTCTCGGAAGATGCTTACAACATGATTTGTGAATTCTTGGGAGCTAACTGGTACGCGTTGGTTGCAACTGCCGGGCTGGCTGCTCTGTTTGTAATTCACATCATCTACGCTTTCTGGTTGACCATGCAGAACCGTGCAGCTCGCGGTAGCGAACGTTATGCAGTGAACGTTAAACCTAAAACTGTGGAATGGGCATCTCAGAACATGCTTGTCCTGGGTATTGTCGTTATTGCCGGATTGGCTCTTCATTTTGCCAATTTCTGGTACAAAATGCAGTTTGCCGAAATTATCGGTGTTCATGATTTGGGCGCATTCGGTCCTACAGACGGTGCCGCTTACATTCGTGAAACATTCGGATGCCCTGTATTTACAGTGCTTTACCTCATTTGGTTAGCAGCTTTGTGGTTCCACCTGACTCACGGATTCTGGAGTGCTTTGCAGACTTTGGGCTGGAGCAATAAAATCTGGTTCGAGCGTTGGAAGACTATTGGTAACATCTACTCTACTTTGGTAGTTCTTGGTTTTGCAGCAGTAGTCGTTATCTACTTCGCTATGACTGGCGCGCCTGATAGGTGGGCTGCCCTTCCTGGTTGGCTTGGTTTCATCAGCCATCCGCTTGCCCTCATCTGTTACGCCGGCGGTAGCCGGCCAGCCTCGCAGAGCAGGATTCCCGTTGAGCACCGCCAGGTGCGAATAAGGGACAGTGAAGAAGGAACACCCGCTCGCGGGTGGGCCTACTTCACCTATCCTGCCCGGCTGACGCCGTTGGATACACCAAGGAAAGTCTACACGAACCCTTTGGCAAAATCCTGTATATCGTGCGAAAAAGGATGGATATACCGAAAAAATCGCTATAATGACCCCGAAGCAGGGTTATGCAGCGGAAAAGCGGGATTAAAAGTCGGGGATTGGTGAACAAAAAGGTGTTTCTCTCTTTAAGAGAAATATCGTTTTGCTAAACAGTTGATATTGAGGTATCATTTTATCGTAAAAGACATTTTTGCTCAACAATTGCTTGACGGAAATCAACAAATTTTAGCATTTTGTAAAAAAGTCGCTATATAATTTGGTGAATTGGAGTTATTTTCATATTTTTGCATCCCGAAGAGTTTCTCTTAAAGAGAGAAACATCTTTTGCATACCTTTTCCGACCGAATTTTTATGTCGTAAAGAGGGGCTTTGCAGGGGGTGGACTCAGAAAGATGAGAATAGATGACTATTGTAGTTGAAACACATAGAAAGTTGCTGATATACAGACCGATACGCATATCGGGATGAACCATGAGTACGTTCTTTTCTCAAAAAACATAAATATTCGAAAAGAGATGCAATAAATTAAGGAGAGGTTATAATGAACAAAGTAAATATAAAAGATAGTCAAAATTTTATTACTTCAAAATATCACATAGAAAAAATAATGAATTGCATAAGTTTAGATGAAAAAGATAACATCTTTGAAATAGGTGCAGGGAAAGGTCATTTTACTGCTGGATTGGTAAAGAGATGTAATTTTGTAACGGCGATAGAAATTGATTCTAAATTATGTGAGGTAACTCGTAATAAGCTCTTAAATTATCCTAACTATCAAATAGTAAATGATGATATACTGAAATTTACATTTCCTAGCCACAATCCATATAAAATATTTGGCAGCATACCTTACAACATAAGCACAAATATAATTCGAAAAATTGTTTTTGAAAGTTCAGCCACAATAAGTTATTTAATAGTGGAATATGGTTTTGCTAAAATGTTATTAGATACAAACAGATCACTAGCATTGCTGTTAATGGCAGAGGTAGATATTTCTATATTAGCAAAAATTCCTAGGTATTATTTCCATCCAAAACCTAAAGTGGATAGCACATTAATTGTATTAAAAAGAAAGCCAGCAAAAATGGCATTTAAAGAGAGAAAAAAATATGAAACTTTTGTAATGAAATGGGTTAACAAAGAGTACGAAAAACTGTTTACAAAAAATCAATTTAATAAAGCTTTAAAACATGCGAGAATATATGATATAAACAATATTAGTTTCGAACAATTTGTATCGCTATTTAATAGTTATAAAATATTTAACGGCTAAAAACAATAGGCCACATGCAACTGTAAATGTTTACGCGGGTACCGACACCGCGGTGGAGGGGAATTGTGTTACAACCAATTAACCAATTCTGATTAGAAAAACTCATCGAGCATCAAATGAAACTGCAATTTATTCATATCAGGATTATCAATACCATATTTTTGAAAAAGCCGTTTCTGTAATGAAGGAGAAAACTCACCGAGGCAGTTCCATAGGATGGCAAGATCCTGGTATCGGTCTGCGATTCCGACTCGTCCAACATCAATACAACCTATTAATTTCCCCTCGTCAAAAATAAGGTTATCAAGTGAGAAATCACCATGAGTGACGACTGAATCCGGTGAGAATGGCAAAAGCTTATGCATTTCTTTCCAGACTTGTTCAACAGGCCAGCCATTACGCTCGTCATCAAAATCACTCGCATCAACCAAACCGTTATTCATTCGTGATTGCGCCTGAGCGAGGCGAAATACGCGATCGCTGTTAAAAGGACAATTACAAACAGGAATCGAATGCAACCGGCGCAGGAACACTGCCAGCGCATCAACAATATTTTCACCTGAATCAGGATATTCTTCTAATACCTGGAATGCTGTTTTCCCGGGGATCGCAGTGGTGAGTAACCATGCATCATCAGGAGTACGGATAAAATGCTTGATGGTCGGAAGAGGCATAAATTCCGTCAGCCAGTTTAGTCTGACCATCTCATCTGTAACATCATTGGCAACGCTACCTTTGCCATGTTTCAGAAACAACTCTGGCGCATCGGGCTTCCCATACAATCGATAGATTGTCGCACCTGATTGCCCGACATTATCGCGAGCCCATTTATACCCATATAAATCAGCATCCATGTTGGAATTTAATCGCGGCCTGGAGCAAGACGTTTCCCGTTGAATATGGCTCATAACACCCCTTGTATTACTGTTTATGTAAGCAGACAGTTTTATTGTTCATGATGATATATTTTTATCTTGTGCAATGTAACATCAGAGATTTTGAGACACAACGTGGCTTTGTTGAATAAATCGAACTTTTGCTGAGTTGAAGGATCAGAGCCTACGTTCCGAATACGGTCAAAAAAAAGGCCATCCGTCAGGATGGCCTTCGCATTAATATGCCGCTTCGAATTCTTTTAGGAAGCGTGTATCGTTTTCAGAGAACATACGGAGGTCTTTCACCTGATATTTCAGGTTTGTGATACGCTCGATACCCATACCGAGTCCATAACCGCTGTATATTTTGCTGTCTATACCATTTGATTCAAGTACGTTCGGGTCTACCATACCGCAACCGAGGATTTCTACCCAGCCGGTGTGTTTACAGAACGGACATCCTTTACCGCCGCAGATATTACAGCTGATATCCATTTCCGCACTTGGTTCAGCAAACGGGAAGTAAGACGGACGCAGACGGATCTTTGTATCAGCACCGAACATTTCTTTGGCAAAGAGCAGCAATACCTGCTTCAAGTCGGTGAATGATACGTTTTTATCTACATACAGCGCTTCTACCTGATGGAAGAAACAGTGTGCGCGATAGCTGATAGCTTCGTTACGATATACACGTCCCGGACAGATGATGCGGATAGGAGGCTGTGAAGTTTCCATCACACGAGTCTGTACAGAAGAAGTATGTGTACGCAATACTACGTCCGGGTGAGCTTCGATAAAGAAAGTGTCCTGCATATCGCGTGCCGGATGATCTTCGGCAAAGTTCAGTGCCGAGAACACGTGCCAGTCATCTTCAATTTCCGGACCTTCGGCAATGCTGAATCCCAGACGGGCAAAGATATCAATGATTTCGTTCTTTACAATGGTGAGCGGGTGGCGTGTACCGAGTTCTACAGGATAAGCCGAACGCGTCAAATCCAGTCCGTCACAATCGTTGTCCTGACTTTCAAACATTTCTTTCAGCGCGTTGATTTTGTCCTGCGCTTTTGTTTTCAGTTCATTCAGTCTCATGCCGACTTCTTTTTTCTGTTCGGCAGCTACATTACGGAAATCTGCCATTAAGTCGTTAATGGCTCCCTTCTTACTTAGGTATTTGATGCGGAGAGCTTCGAGTTCTTCGGCATTGGAGGCGTGTAAGGCTTCCACCTCTTTCAGAAGTTGTTCAATCTTAGCTATCATTTTTTAATATTTTTAGCGGCCCCGTTAAACAAAATTATTTGTAGAGGCTGTTTCGTCCTCACGGACTCATCAGACCGGAAAGCACATCCGGTGACAGCTCAGGCTACTTTGTTTCTTTCGACACTGCAAATATAAGAACATTATTTGAAAGTTCAAGTGAAACTTTAAATTTTAACAATAGATTAACCATTGCAAACAAAACAAAAAAAAGGTAGCCCAATTGTAAAACGAAAGGCCCAGTCTTTCGACTGAGCCTTTCGTTTTATCCTACAGTCGCTCGGCGATCGAAGGCTTCGGAAAAAAAAGGCCATCCGTCAGGATGGCCTTCGCATTAATATGCCGCTTCGAATTCTTTTAGGAAGCGTGTATCGTTTTCAGAGAACATACGGAGGTCTTTCACCTGATATTTCAGGTTTGTGATACGCTCGATACCCATACCGAGTCCATAACCGCTGTATATTTTGCTGTCTATACCATTTGATTCAAGTACGTTCGGGTCTACCATACCGCAACCGAGGATTTCTACCCAGCCGGTGTGTTTACAGAACGGACATCCTTTACCGCCGCAGATATTACAGCTGATATCCATTTCCGCACTTGGTTCAGCAAACGGGAAGTAAGACGGACGCAGACGGATCTTTGTATCAGCACCGAACATTTCTTTGGCAAAGAGCAGCAATACCTGCTTCAAGTCGGTGAATGATACGTTTTTATCTACATACAGCGCTTCTACCTGATGGAAGAAACAGTGTGCGCGATAGCTGATAGCTTCGTTACGATATACACGTCCCGGACAGATGATGCGGATAGGAGGCTGTGAAGTTTCCATCACACGAGTCTGTACAGAAGAAGTATGTGTACGCAATACTACGTCCGGGTGAGCTTCGATAAAGAAAGTGTCCTGCATATCGCGTGCCGGATGATCTTCGGCAAAGTTCAGTGCCGAGAACACGTGCCAGTCATCTTCAATTTCCGGACCTTCGGCAATGCTGAATCCCAGACGGGCAAAGATATCAATGATTTCGTTCTTTACAATGGTGAGCGGGTGGCGTGTACCGAGTTCTACAGGATAAGCCGAACGCGTCAAATCCAGTCCGTCACAATCGTTGTCCTGACTTTCAAACATTTCTTTCAGCGCGTTGATTTTGTCCTGCGCTTTTGTTTTCAGTTCATTCAGTCTCATGCCGACTTCTTTTTTCTGTTCGGCAGCTACATTACGGAAATCTGCCATTAAGTCGTTAATGGCTCCCTTCTTACTTAGGTATTTGATGCGGAGAGCTTCGAGTTCTTCGGCATTGGAGGCGTGTAAGGCTTCCACCTCTTTCAGAAGTTGTTCAATCTTAGCTATCATTTTTTAATATTTTTAGCGGCCCCGTTAAACAAAATTATTTGTAGAGGCTGTTTCGTCCTCACGGACTCATCAGACCGGAAAGCACATCCGGTGACAGCTCAGGCTACTTTGTTTCTTTCGACACTGCAAATATAAGAACATTATTTGAAAGTTCAAGTGAAACTTTAAATTTTAACAATAGATTAACCATTGCAAACAAAACAAAAAAAAGGTAGCCCAATTGTAAAACGAAAGGCCCAGTCTTTCGACTGAGCCTTTCGTTTTATCCTAGGATCAGCTGTACGTACTCGCAGTTCAACCTGTTGATAGTACGTACTAAGCTCTCATGTTTCACGTACTAAGCTCTCATGTTTAACGTACTAAGCTCTCATGTTTAACGAACTAAACCCTCATGGCTAACGTACTAAGCTCTCATGGCTAACGTACTAAGCTCTCATGTTTCACGTACTAAGCTCTCATGTTTGAACAATAAAATTAATATAAATCAGCAACTTAAATAGCCTCTAAGGTTTTAAGTTTTATAAGAAAAAAAAGAATATATAAGGCTTTTAAAGCTTTTAAGGTTTAACGGTTGTGGACAACAAGCCAGGGATGTAACGCACTGAGAAGCCCTTAGAGCCTCTCAAAGCAATTTTGAGTGACACAGGAACACTTAACGGCTGACATGGGGCGGCCGCGTAG
SEQ ID NO:89(用于基因组整合进普通拟杆菌基因组中的编码草酸盐降解途径的质粒)：
GGAGAGCTGTATGTGGGGTGGAAGAAGCTATCGTAAAAATGCTGGAGCAGCTTGATCCTCATTCTACCTATTCTGATCCGGAGGAAGTGAAGAGAATGAACGAGCCGTTGCAGGGAAACTTCGAAGGTATCGGTATACAGTTTAATATGGCGGAGGACACTTTGCTGGTGATACAGCCTGTATCGGGCGGACCGTCCGAAAAAGTCGGTATTCTGGCCGGTGATCGTATCGTCATGGTGGAAGATACGCTGATAGCCGGTGTAAAGATGAGTACCGAGGATATTATGCGTCGCCTCCGTGGTCCTAAAGATTCAAAAGTGAATCTGAAAATACTTCGCCGAGGGGTGAAGGAACTGCTCCCCTTTACGGTGAAAAGGGATAAAATACCTGTTTACAGCTTGGATGCTTCCTATATGATTAAAGATAAGATCGGTTATATTCGTATCAACCGTTTTGCCGCCACTACCCATGAGGAGTTCAAGAAAGCGCTGACCGGGTTGCAGAAGAAAGGAATGAAAGACTTGATTCTTGATTTGCAGGGCAATGGCGGGGGGTATTTGAATGCTGCTATCGATATTGCCAATGAATTTTTGGGTAAAAAGGAACTGATTGTCTATACGGAGGGCCGTCGTAATCCTCGTAGCGAGTTTTTTGCCAAAGGTACGGGCGAGTTTCAGAACGGTCGTCTGATGGTATTGGTTGATGAATTTTCGGCTTCTGCCAGTGAGATTGTGACCGGAGCTGTTCAGGATTGGGACAGAGGCGTTGTGGTAGGCCGTCGCAGTTTTGGTAAAGGCTTGGTGCAGCGTCCCATTGATTTGCCGGACGGTTCGATGATCCGTCTGACGGTGGCCCGCTATTATACGCCTGCCGGACGTTGTATCCAGAAACCTTACGAGAGCATTGAAAAGTATAATGAAGACCTGATAGACCGTTATAATAAAGGAGAAATGATGAGTGAAGACAGCATCCACTTTCCGGATTCACTGAAGTTCAAGACTCATAGGTTGGCCCGTACGGTATATGGTGGCGGAGGGATCATGCCCGATTATTTTGTGCCGATTGATACGACTCTCTATACTAAATACCATCGTCAGTTGCGTGATAAAGGAGCATTGATGAAAGCTCATTTCCATTTTATTGATGCCCATCGTAAAGAATGGCTGGGCAAATACAAGACTTTCAATGAGTTCTACAAACGTTTTGAAGTGACTCCGGATATGTTGACACAACTGGTTGCTACCGGCAAGGAGATGGGAGTGGAATATAATGAAGAAGAGTATCAAAAGGCCCTGCCTCTGCTGAGGTTGCAGATGAAAGCATTGATAGCCCGTGATTTATGGGATATGAATGAATATTATCACGTCATTAATGACGCGAATGAAAGTATCAGGAAAGCGCTGGAACTGTTGGAGCAACCGGACTTTGAGGGGCTGCTGTTGAAAAAGCGATAGTCAAGACTTTATATTAACTGCAGGGGCGCATAGTTGCACTCCTGCAGTTGTCTGAAAAACTTGTAGGATAAAACGAAAGGCTCAGTCGAAAGACTGGGCCTTTCGTTTTACAATTGGGCTACCTTTTTTTTGTTTTGTTTGCAATGGTTAATCTATTGTTAAAATTTAAAGTTTCACTTGAACTTTCAAATAATGTTCTTATATTTGCAGTGTCGAAAGAAACAAAGTAGCCTGGCATCATCTACCTTCATTATTAATCATTCCAATTGTATCACTTAAAAATATAAAAAAATGTCAACTCCACTTCAAGGAATTAAAGTTCTCGATTTCACCGGTGTGCAATCTGGCCCATCTTGTACTCAAATGCTGGCCTGGTTTGGCGCTGACGTTATTAAAATTGAACGTCCCGGCGTTGGTGACGTAACGCGTCACCAGCTGCGAGATATTCCTGATATCGATGCGCTTTACTTCACCATGCTTAACAGTAACAAACGTTCTATTGAGTTAAATACCAAAACAGCGGAAGGCAAAGAGGTAATGGAAAAGCTGATCCGCGAAGCTGATATCTTAGTCGAGAACTTTCATCCAGGGGCCATTGATCACATGGGCTTCACCTGGGAGCATATTCAAGAAATCAATCCACGTCTGATTTTTGGTTCGATCAAAGGGTTTGATGAGTGTTCGCCTTATGTGAATGTAAAAGCCTATGAAAACGTTGCTCAGGCAGCGGGTGGCGCGGCATCCACTACGGGTTTTTGGGATGGTCCGCCGCTGGTAAGCGCTGCAGCGTTGGGTGACAGCAACACCGGAATGCATTTGCTGATCGGTTTACTTGCTGCTTTGCTGCATCGCGAAAAAACGGGGCGTGGGCAACGAGTCACCATGTCAATGCAGGATGCCGTATTGAACCTTTGCCGCGTGAAATTACGTGACCAGCAGCGTCTAGATAAATTGGGTTATCTGGAAGAATACCCGCAGTATCCGAATGGTACATTTGGTGATGCAGTTCCCCGCGGTGGTAATGCAGGTGGTGGCGGTCAGCCTGGCTGGATCCTGAAATGTAAAGGCTGGGAAACCGATCCTAACGCCTATATTTATTTCACTATTCAGGAGCAAAACTGGGAAAACACCTGTAAAGCCATCGGCAAACCAGAATGGATTACCGATCCGGCATACAGTACAGCCCATGCACGACAGCCACATATTTTCGATATTTTTGCTGAAATCGAAAAATACACTGTCACTATTGATAAACATGAAGCGGTGGCCTATTTGACTCAGTTTGATATTCCTTGTGCACCGGTTTTAAGTATGAAAGAAATTTCACTTGATCCCTCTTTGCGCCAAAGTGGCAGTGTTGTTGAAGTGGAACAACCGTTGCGTGGAAAATATCTGACCGTTGGTTGTCCAATGAAATTCTCTGCCTTTACGCCGGATATTAAAGCTGCGCCGCTATTAGGTGAACATACCGCTGCTGTATTGCAGGAGCTGGGTTATAGCGACGATGAAATTGCTGCAATGAAGCAAAACCACGCCATCTGAGGTTCCTAACCTGCCTGGCATCTACCCCTAACAACCCATAAATTTAACTTTAAAAAATTTTTAAATATAATAAAATGACTACCACAGCAACGCAGCATTTTGCCCAGTTACTCCAGAAGTACGGAATTGATACTGTCTTCGGCATAGTAGGTATTCCGATCGTGCAGTTGGCCGACACTATGGTCGCGAATGGAATTAAGTTTATACCGTGTCGCAATGAGCAAGCCGCAAGTTACGCGGCAAGCGCCTACGGCTACATCTCGGACAAACCTGGTGTCCTGTTAATCGTGGGAGGGCCTGGCCTTATTCATGCCTTAGCGGGGATCTATAATAGTATGTCGAACCGCTGGCCCTTGTTGGTCATCGCCGGATCCAGCAGCCAGTCAGACATCCACAAGGGAGGTTTCCAGGAACTTGACCAGGTGTCCTTGCTAAGTCCGTTCCTAAAATTCACTGGTAAACTGACGCCGGATAACATCGATATGATAACCCAAAAAGCACTCAACTATTGCATTCAGGGAACCGCCGGAGTGAGCTATATCGATGTCCCCGCTGACTTCATCGAATACGAGAAGCCGCTTGAGGGCAATGATAGAACTGGTAACGAGCTGCCAATGATCCTGACGCCCAATATCTGCGGTCCAGACCCCAGTAAAATTAAGAAGGTGGTGCAATTGATACTCCAGCACAAGAACAAGAACATATTAATAGTGATAGGTAAAGGAGCAGTGAAGAATAGCCATGAGATTAGACGCTTGGTTAACACCTTTAACTTGCCGTTCCTTCCGACTCCCATGGCGAAAGGCATAGTGCCCGATAGCAGCCCTTTAAATGTGAGCAGCGCCCGGAGCCAGGCTCTTAAGATTGCTGACATCGTGTTAGTATTGGGAGCTCGCCTGAATTGGATCCTTCACTTCGGAACGAGTCCAAAGTGGAATAGCGAGAGTATTTTTATACAATTTGACAGTAACCCGGAAACGCTCGGAGACAATAACGTTAGTCCCGGAGCAGATTTAAGTATCTGGGGGGACATCGGACTATCGGTTACTGCACTGGTAGAAGAGCTCACGAGACAGGACAGTTGTTGGAAATACTCGGGAGTCAAACAGGAGATCAGGGAAAAGATACAGTTAAACCAGACCAGATTGCTCCGTAAGGAAAAAACGCGTGGAGCTCAGCTTAACTATAATCAAGTGTACGGCACCCTTCGTCCGTTAATTGACGACTATCGCACGATCTTGGTCACAGAAGGTGCCAACACAATGGACATCGCTAGAATAAGTTTTCCGACTGATGCACCTCGCAGAAGACTTGATGCTGGTACAAATGCTACAATGGGTATAGGTTTAGGTTACGCATTAGCGTGTAAAGCCAGCCACCCTGAGTTAGACGTTGTTTTAATCCAAGGCGACAGTGCCTTCGGTTTTAGCGCTATGGAGATCGAGACTGCCGTCCGTTGCCAATTAGCCCTTGTGATTGTCGTAATGAATAACTCTGGGATATATCACGGTGAGAAAGACATCGAAGGAGATCTGCCCCCGACCGCTTTGTCAAAAAATTGTAGATATGACTTGGTCGGTAAGGGTTTAGGAGCTAACGACTTCTTCGTCAATACCATCTCAGAGTTATCTCGCTGTTTTCAGCAGGCAGTTCAATTGAGCAGAACCAAGCGCGAAACCTCCGTCATTAATGTCATAATCGAACCGGGAGAGCAGAAACAGATCGCGTTCGCATGGCAAAACAAGCCTAGACTGTAAGGTTCCTAAACGACCTGGCATCATCTACCTATATTACTAAACAAACCAATTATAATAATTTTAAAAATAAAAAAATGACAAATAATGAAAGCAAAGGGCCGTTTGAAGGCTTATTAGTTATCGATATGACACATGTCCTTAATGGACCTTTCGGAACTCAACTTCTTTGTAATATGGGCGCAAGGGTAATTAAAGTTGAGCCGCCGGGTCATGGTGATGATACCCGCACATTTGGTCCCTATGTGGATGGACAGTCACTCTATTACAGTTTTATTAATCATGGCAAAGAGAGTGTGGTTCTTGATTTAAAGAATGATCACGATAAAAGTATATTTATAAATATGCTCAAACAAGCTGATGTATTAGCTGAGAATTTTCGCCCAGGTACAATGGAAAAACTGGGGTTTTCATGGGAAACGCTTCAAGAAATCAACCCGCGCCTCATATATGCTTCATCGTCAGGTTTCGGACATACCGGTCCGCTAAAAGATGCTCCTGCCTACGATACCATCATTCAGGCAATGAGCGGGATAATGATGGAAACAGGATATCCTGATGCTCCGCCAGTGCGCGTTGGTACATCTCTTGCGGATCTATGCGGCGGTGTCTATTTATTCAGCGGAATAGTGAGTGCACTTTATGGCCGCGAAAAGAGCCAGAGAGGGGCGCATGTCGATATAGCGATGTTTGATGCCACGCTGAGTTTTCTGGAGCATGGTCTGATGGCATATATCGCAACTGGGAAGTCACCACAACGTCTGGGAAATCGCCATCCCTACATGGCACCTTTTGATGTTTTCAATACTCAGGATAAGCCGATTACGATTTGTTGTGGTAATGACAAGCTTTTTTCTGCGTTATGCCAGGCACTGGAGCTTACGGAACTGGTTAATGATCCCCGATTTAGCAGCAATATTTTACGCGTACAAAACCAGGCTATTCTTAAACAATATATTGAGCGGACGTTAAAAACGCAGGCAGCTGAAGTTTGGTTAGCCAGAATACATGAAGTTGGTGTACCCGTCGCGCCGTTATTAAGTGTGGCTGAGGCCATTAAATTGCCACAAACTCAGGCGAGAAATATGTTGATTGAAGCCGGGGGAATAATGATGCCGGGTAATCCGATAAAAATCAGCGGCTGCGCGGACCCGCATGTTATGCCGGGAGCGGCAACGCTCGACCAGCATGGGGAACAAATTCGCCAGGAGTTCTCATCATAAGGTTCCTAAGGGTCCTGGCATCTAAACTAAAATACAATAACCAAAAATCATCTATGCGTAAAAAAAATTATAAAATGGATAGCGATACTCTCTCAGGATTATTGGAAAACGTCGCCAAAAAATTCCCCGATCGCCGAGCTCTCTCCGTTTCTGGAAAATTCAATCTCACTCACGCGCGTCTTCACGATCTAATCGAACGCGCCGCTTCACGCCTTGTCTCCGACGCTGGAATCAAACCCGGCGATGTCGTTGCTCTCACCTTCCCTAACACCGTCGAGTTTGTTATAATGTTTTTAGCGGTGATAAGAGCTAGAGCCACGGCGGCGCCGTTGAACGCAGCGTACACGGCGGAGGAATTTGAGTTTTACCTCTCCGATTCAGATTCAAAGCTATTGTTAACCTCTAAAGAAGGAAACGCACCGGCTCAAGAAGCAGCTTCAAAGCTGAAAATCTCTCACGTCACCGCTACGCTGCTTGACGCTGGCTCGGACCTTGTACTATCCGTTGCGGATTCAGATTCCGTCGTTGACTCAGCGACGGAACTCGTTAATCACCCGGACGACGGTGCTCTCTTCCTCCACACTTCTGGCACTACGAGCCGTCCAAAGGGTGTACCGCTTACGCAGCTCAATCTAGCTTCATCCGTCAAGAACATTAAAGCTGTGTACAAGCTTACTGAGTCTGATTCTACGGTGATTGTTCTCCCTCTGTTCCATGTTCATGGATTGTTAGCTGGGTTGCTTAGCTCGCTTGGAGCTGGTGCTGCTGTAACTCTTCCAGCTGCTGGTAGATTCTCAGCAACAACATTTTGGCCAGATATGAAGAAGTATAACGCTACATGGTATACTGCTGTGCCGACCATTCATCAGATCATATTGGACCGCCACGCGAGCCACCCTGAAACGGAATATCCTAAACTCCGGTTCATCAGGAGTTGCAGTGCTTCTTTGGCTCCGGTGATATTGTCCAGGCTTGAGGAAGCGTTTGGAGCACCGGTGCTCGAGGCCTATGCAATGACAGAGGCAACACATTTGATGAGCTCAAACCCCTTACCAGAGGAAGGTCCACACAAGCCTGGGTCTGTTGGGAAACCGGTAGGTCAAGAAATGGCGATCCTTAATGAGAAAGGCGAGATCCAAGAGCCAAATAACAAAGGAGAGGTTTGTATAAGAGGTCCAAATGTGACCAAGGGTTACAAGAATAACCCAGAAGCCAACAAGGCAGGTTTCGAGTTTGGGTGGTTCCACACTGGTGATATCGGTTACTTTGATACCGATGGGTATTTGCATCTGGTGGGTCGGATCAAAGAGCTTATTAACCGTGGAGGTGAGAAGATATCTCCAATTGAAGTGGATGCAGTACTCTTAACGCATCCTGACGTTTCTCAGGGTGTTGCATTCGGTGTTCCTGATGAGAAATATGGGGAAGAGATTAACTGTGCGGTGATTCCAAGAGAAGGAACTACTGTAACCGAAGAGGACATTAAAGCGTTTTGTAAGAAGAATTTGGCAGCTTTCAAGGTGCCAAAGAGAGTGTTCATCACTGATAACCTCCCCAAAACTGCCTCTGGTAAGATTCAGCGCCGTATCGTCGCACAACATTTCCTTGAGAAGCCCTGAGGTTCCTAATTCCCCTGGCATCCCCAAAAAATTAATTCATTTTCTCTTTTCAACAATTTAAAAAAAATTAAAAAATGAGTAACGACGACAATGTAGAGTTGACTGATGGCTTTCATGTTTTGATCGATGCCCTGAAAATGAATGACATCGATACCATGTATGGTGTTGTCGGCATTCCTATCACGAACCTGGCTCGTATGTGGCAAGATGACGGTCAGCGTTTTTACAGCTTCCGTCACGAACAACACGCAGGTTATGCAGCTTCTATCGCCGGTTACATCGAAGGAAAACCTGGCGTTTGCTTGACCGTTTCCGCCCCTGGCTTCCTGAACGGCGTGACTTCCCTGGCTCATGCAACCACCAACTGCTTCCCAATGATCCTGTTGAGCGGTTCCAGTGAACGTGAAATCGTCGATTTGCAACAGGGCGATTACGAAGAAATGGATCAGATGAATGTTGCACGTCCACACTGCAAAGCTTCTTTCCGTATCAACAGCATCAAAGACATTCCAATCGGTATCGCTCGTGCAGTTCGCACCGCTGTATCCGGACGTCCAGGTGGTGTTTACGTTGACTTGCCAGCAAAACTGTTCGGTCAGACCATTTCTGTAGAAGAAGCTAACAAACTGCTCTTCAAACCAATCGATCCAGCTCCGGCACAGATTCCTGCTGAAGATGCTATCGCTCGCGCTGCTGACCTGATCAAGAACGCCAAACGTCCAGTTATCATGCTGGGTAAAGGCGCTGCATACGCACAATGCGACGACGAAATCCGCGCACTGGTTGAAGAAACCGGCATCCCATTCCTGCCAATGGGTATGGCTAAAGGCCTGCTGCCTGACAACCATCCACAATCCGCTGCTGCAACCCGTGCTTTCGCACTGGCACAGTGTGACGTTTGCGTACTGATCGGCGCTCGTCTGAACTGGCTGATGCAGCACGGTAAAGGCAAAACCTGGGGCGACGAACTGAAGAAATACGTTCAGATCGACATCCAGGCTAACGAAATGGACAGCAACCAGCCTATCGCTGCACCAGTTGTTGGTGACATCAAGTCCGCCGTTTCCCTGCTCCGCAAAGCACTGAAAGGCGCTCCAAAAGCTGACGCTGAATGGACCGGCGCTCTGAAAGCCAAAGTTGACGGCAACAAAGCCAAACTGGCTGGCAAGATGACTGCCGAAACCCCATCCGGAATGATGAACTACTCCAATTCCCTGGGCGTTGTTCGTGACTTCATGCTGGCAAATCCGGATATTTCCCTGGTTAACGAAGGCGCTAATGCACTCGACAACACTCGTATGATTGTTGACATGCTGAAACCACGCAAACGTCTTGACTCCGGTACCTGGGGTGTTATGGGTATTGGTATGGGCTACTGCGTTGCTGCAGCTGCTGTTACCGGCAAACCGGTTATCGCTGTTGAAGGCGATAGCGCATTCGGTTTCTCCGGTATGGAACTGGAAACCATCTGCCGTTACAACCTGCCAGTTACCGTTATCATCATGAACAATGGTGGTATCTATAAAGGTAACGAAGCAGATCCACAACCAGGCGTTATCTCCTGTACCCGTCTGACCCGTGGTCGTTACGACATGATGATGGAAGCATTTGGCGGTAAAGGTTATGTTGCCAATACTCCAGCAGAACTGAAAGCTGCTCTGGAAGAAGCTGTTGCTTCCGGCAAACCATGCCTGATCAACGCGATGATCGATCCAGACGCTGGTGTCGAATCTGGCCGTATCAAGAGCCTGAACGTTGTAAGTAAAGTTGGCAAGAAATAAGGTTCCTAAAATGCCTGGCATCAACTACCTCAATTAATAATCAAACCATCTGAACAATGAATAAATTTAAAAAAATGACAAGTGCCGCTACTGTTACTGCTTCGTTCAACGATACTTTTAGCGTATCCGATAATGTCGCCGTTATTGTTCCTGAAACGGACACTCAGGTGACCTACAGGGATCTATCCCACATGGTGGGTCACTTCCAGACCATGTTCACAAATCCTAATTCTCCATTGTACGGAGCTGTTTTCAGACAAGATACAGTGGCGATATCCATGCGTAATGGGCTGGAATTTATCGTCGCTTTCCTCGGTGCTACTATGGACGCTAAAATTGGCGCGCCCTTGAATCCCAATTATAAGGAAAAGGAGTTCAATTTTTATTTGAATGACCTGAAATCTAAGGCGATTTGCGTCCCAAAGGGTACCACAAAGTTACAGAGTTCTGAAATTCTAAAATCTGCCTCCACGTTTGGATGTTTTATCGTAGAGCTGGCCTTCGATGCGACCAGGTTTAGGGTAGAGTATGATATATACTCTCCAGAGGACAACTACAAAAGGGTTATTTACCGGTCTTTGAACAACGCCAAATTTGTCAACACAAATCCCGTTAAATTCCCTGGGTTTGCCCGTTCCAGTGACGTTGCCCTGATTTTGCATACCAGTGGTACCACCTCCACTCCAAAAACGGTGCCTTTGTTACATTTGAACATTGTGAGAAGCACGTTGAACATTGCTAACACTTACAAGCTAACGCCCTTGGACAGATCTTATGTCGTGATGCCTCTTTTCCACGTCCATGGGTTAATTGGTGTTTTACTTTCCACTTTTAGAACTCAGGGTTCTGTTGTGGTTCCCGATGGATTCCATCCAAAGTTATTCTGGGACCAATTTGTTAAGTACAACTGTAATTGGTTCAGTTGCGTTCCCACAATAAGCATGATTATGCTGAACATGCCCAAACCAAACCCTTTCCCACACATTAGATTCATCAGATCGTGTTCTTCTGCTTTGGCTCCAGCAACGTTCCATAAGCTGGAGAAGGAATTCAATGCACCTGTCTTGGAGGCCTATGCGATGACCGAAGCATCACATCAAATGACCTCAAACAATCTGCCTCCAGGAAAGAGAAAGCCTGGTACTGTGGGCCAGCCACAAGGAGTCACCGTCGTCATTCTAGATGACAATGACAATGTCTTGCCCCCGGGCAAAGTCGGCGAAGTTTCCATCAGAGGCGAAAACGTCACTTTGGGGTATGCTAATAATCCAAAAGCTAACAAGGAGAACTTCACCAAGAGAGAGAACTATTTCAGAACCGGTGACCAAGGTTATTTCGACCCTGAGGGGTTTTTGGTCCTTACAGGCAGAATCAAAGAGCTTATCAACAGGGGTGGTGAAAAGATTTCACCCATTGAGCTCGACGGCATTATGCTATCGCATCCAAAGATCGATGAAGCCGTTGCATTTGGTGTTCCCGACGATATGTACGGCCAAGTAGTTCAAGCCGCCATTGTTTTGAAGAAGGGAGAAAAAATGACCTACGAAGAACTGGTGAACTTCTTAAAGAAGCACCTAGCCTCTTTCAAAATTCCAACCAAGGTGTACTTTGTTGATAAGCTACCAAAAACCGCTACAGGTAAAATCCAGAGAAGAGTTATCGCAGAAACTTTTGCTAAGAGCAGCAGAAATAAGAGTAAGTTGTAGGGTTCCTAAAAACCCTGGCATCATCTACCTATATTATTCATCAAACCATTTGATCCAACATTAAATATTAAAAAATGAATAATCCACAAACAGGACAATCAACAGGCCTCTTGGGCAATCGTTGGTTCTACTTGGTATTAGCAGTTTTGCTGATGTGTATGATCTCGGGTGTCCAATATTCCTGGACACTGTACGCTAACCCGGTTAAAGACAACCTTGGCGTTTCTTTGGCTGCGGTTCAGACGGCTTTCACACTCTCTCAGGTCATTCAAGCTGGTTCTCAGCCTGGTGGTGGTTACTTCGTTGATAAATTCGGTCCAAGAATTCCATTGATGTTCGGTGGTGCGATGGTTCTCGCTGGCTGGACCTTCATGGGTATGGTTGACAGTGTTCCTGCTCTGTATGCTCTTTATACTCTGGCCGGTGCAGGTGTTGGTATCGTTTACGGTATCGCGATGAACACGGCTAACAGATGGTTCCCGGACAAACGCGGTCTGGCTTCCGGTTTCACCGCTGCCGGTTACGGTCTGGGTGTTCTGCCGTTCCTGCCACTGATCAGCTCCGTTCTGAAAGTTGAAGGTGTTGGCGCAGCATTCATGTACACCGGTTTGATCATGGGTATCCTGATTATCCTGATCGCTTTCGTTATCCGTTTCCCTGGCCAGCAAGGCGCCAAAAAACAAATCGTTGTTACCGACAAGGATTTCAATTCTGGCGAAATGCTGAGAACACCACAATTCTGGGTTCTGTGGACCGCATTCTTTTCCGTTAACTTTGGTGGTTTGCTGCTGGTTGCCAACAGCGTCCCTTACGGTCGCAGCCTCGGTCTTGCCGCAGGTGTGCTGACGATCGGTGTTTCGATCCAGAACCTGTTCAATGGTGGTTGCCGTCCTTTCTGGGGTTTCGTTTCCGATAAAATCGGCCGTTACAAAACCATGTCCGTCGTTTTCGGTATCAATGCTGTTGTTCTCGCACTTTTCCCGACGATTGCTGCCTTGGGCGATGTAGCCTTTATCGCCATGTTGGCAATCGCATTCTTCACATGGGGTGGTAGCTACGCTCTGTTCCCATCGACCAACAGCGATATTTTCGGTACGGCATACTCTGCCAGAAACTATGGTTTCTTCTGGGCTGCAAAAGCAACTGCCTCGATCTTCGGTGGTGGTCTGGGTGCTGCAATTGCAACCAACTTCGGATGGAATACCGCTTTCCTGATTACTGCGATTACTTCTTTCATCGCATTTGCTCTGGCTACCTTCGTTATTCCAAGAATGGGCCGTCCAGTCAAGAAAATGGTCAAATTGTCTCCAGAAGAAAAAGCTGTACATTAAGGTTCCTAGCTGATTAGAAGGCCATCCTGACGGATGGCCTTTTTTTTGACTCTGTTGTGTCTTCTTTTAGGTGTGGAATAATTTTCCAACCATCACTTTATCTGTCTCCCGAACAGCACCTTATTCAGCCATCGGTTCACATACACGTTCACGAAAGCACATCCTACTCCGATAGCGGCTCCTGCCACTACATCACTGGGATAGTGCACCCCTTGGTTCATCCGGGCAAACCCTACTCCGCAAGCCCATACGGCTGAAGGGGCGATAACATACCACTTAGGATAAGTGATGGAGAGTGAGGTGGCTAAAGAAAAAGCTGCCGCAGTGTGTCCTGAAGGGAATGACGGACTATCGGCATCCGGTGCTCCGTATGCATGAATCTTATCCGGATACTTTACAAAAGGCCGGTCACGACCTATTATATGTTTGGCGGCGTATGTAATCCCCACTGCTTCAATCACACTGGTTCCTATGTAAATGGCATCTTTCAGCATCGGCTGGTCTTTCTTTATCAATGCATAGAGTCCCATGGCTGTAGGTATTCCTACGGGTAGAATCACTCCGCTAGCGGACAATCCGCGGCTCAGTCCGTGTACATCCCATCGGTTGATGGTTTTCAACGTGTTGATGTCTATATTCTGTGCGCTGAGGCTGAATGTAAAACAGGCTGTCAGTATAAGAAAAATAATCCTTTGGCGTTTCATTTAGTTTGGCATTTGTTCAGTGCAAAGATAGGAAAATAATGTAATTCGCATTTACCCTTCCCCGAATTAAAGAATAATTTCCATAATTCCTTGTATTATCGCTAGAATTCCTTTACTTTTGCATCGTGAATACACAGCTTTTAACAGAATTTTTAGTTCTAACAATTAAATAATTTAAATTCAATCATTTATGTGGTTAAGTAATTCATCTATTGGAAGGAAAGTGGTGATGAGTGTCACAGGTATCGCCCTTGTCCTGTTTCTGACGTTTCACATGGCGATGAACCTTGTTGCGCTCTTCTCGGAAGATGCTTACAACATGATTTGTGAATTCTTGGGAGCTAACTGGTACGCGTTGGTTGCAACTGCCGGGCTGGCTGCTCTGTTTGTAATTCACATCATCTACGCTTTCTGGTTGACCATGCAGAACCGTGCAGCTCGCGGTAGCGAACGTTATGCAGTGAACGTTAAACCTAAAACTGTGGAATGGGCATCTCAGAACATGCTTGTCCTGGGTATTGTCGTTATTGCCGGATTGGCTCTTCATTTTGCCAATTTCTGGTACAAAATGCAGTTTGCCGAAATTATCGGTGTTCATGATTTGGGCGCATTCGGTCCTACAGACGGTGCCGCTTACATTCGTGAAACATTCGGATGCCCTGTATTTACAGTGCTTTACCTCATTTGGTTAGCAGCTTTGTGGTTCCACCTGACTCACGGATTCTGGAGTGCTTTGCAGACTTTGGGCTGGAGCAATAAAATCTGGTTCGAGCGTTGGAAGACTATTGGTAACATCTACTCTACTTTGGTAGTTCTTGGTTTTGCAGCAGTAGTCGTTATCTACTTCGCTATGACTGGCGCGCCTGATAGGTGGGCTGCCCTTCCTGGTTGGCTTGGTTTCATCAGCCATCCGCTTGCCCTCATCTGTTACGCCGGCGGTAGCCGGCCAGCCTCGCAGAGCAGGATTCCCGTTGAGCACCGCCAGGTGCGAATAAGGGACAGTGAAGAAGGAACACCCGCTCGCGGGTGGGCCTACTTCACCTATCCTGCCCGGCTGACGCCGTTGGATACACCAAGGAAAGTCTACACGAACCCTTTGGCAAAATCCTGTATATCGTGCGAAAAAGGATGGATATACCGAAAAAATCGCTATAATGACCCCGAAGCAGGGTTATGCAGCGGAAAAGCGGGATTAAAAGTCGGGGATTGGTGAACAAAAAGGTGTTTCTCTCTTTAAGAGAAATATCGTTTTGCTAAACAGTTGATATTGAGGTATCATTTTATCGTAAAAGACATTTTTGCTCAACAATTGCTTGACGGAAATCAACAAATTTTAGCATTTTGTAAAAAAGTCGCTATATAATTTGGTGAATTGGAGTTATTTTCATATTTTTGCATCCCGAAGAGTTTCTCTTAAAGAGAGAAACATCTTTTGCATACCTTTTCCGACCGAATTTTTATGTCGTAAAGAGGGGCTTTGCAGGGGGTGGACTCAGAAAGATGAGAATAGATGACTATTGTAGTTGAAACACATAGAAAGTTGCTGATATACAGACCGATACGCATATCGGGATGAACCATGAGTACGTTCTTTTCTCAAAAAACATAAATATTCGAAAAGAGATGCAATAAATTAAGGAGAGGTTATAATGAACAAAGTAAATATAAAAGATAGTCAAAATTTTATTACTTCAAAATATCACATAGAAAAAATAATGAATTGCATAAGTTTAGATGAAAAAGATAACATCTTTGAAATAGGTGCAGGGAAAGGTCATTTTACTGCTGGATTGGTAAAGAGATGTAATTTTGTAACGGCGATAGAAATTGATTCTAAATTATGTGAGGTAACTCGTAATAAGCTCTTAAATTATCCTAACTATCAAATAGTAAATGATGATATACTGAAATTTACATTTCCTAGCCACAATCCATATAAAATATTTGGCAGCATACCTTACAACATAAGCACAAATATAATTCGAAAAATTGTTTTTGAAAGTTCAGCCACAATAAGTTATTTAATAGTGGAATATGGTTTTGCTAAAATGTTATTAGATACAAACAGATCACTAGCATTGCTGTTAATGGCAGAGGTAGATATTTCTATATTAGCAAAAATTCCTAGGTATTATTTCCATCCAAAACCTAAAGTGGATAGCACATTAATTGTATTAAAAAGAAAGCCAGCAAAAATGGCATTTAAAGAGAGAAAAAAATATGAAACTTTTGTAATGAAATGGGTTAACAAAGAGTACGAAAAACTGTTTACAAAAAATCAATTTAATAAAGCTTTAAAACATGCGAGAATATATGATATAAACAATATTAGTTTCGAACAATTTGTATCGCTATTTAATAGTTATAAAATATTTAACGGCTAAAAACAATAGGCCACATGCAACTGTAAATGTTTACGCGGGTACCGACACCGCGGTGGAGGGGAATTGTGTTACAACCAATTAACCAATTCTGATTAGAAAAACTCATCGAGCATCAAATGAAACTGCAATTTATTCATATCAGGATTATCAATACCATATTTTTGAAAAAGCCGTTTCTGTAATGAAGGAGAAAACTCACCGAGGCAGTTCCATAGGATGGCAAGATCCTGGTATCGGTCTGCGATTCCGACTCGTCCAACATCAATACAACCTATTAATTTCCCCTCGTCAAAAATAAGGTTATCAAGTGAGAAATCACCATGAGTGACGACTGAATCCGGTGAGAATGGCAAAAGCTTATGCATTTCTTTCCAGACTTGTTCAACAGGCCAGCCATTACGCTCGTCATCAAAATCACTCGCATCAACCAAACCGTTATTCATTCGTGATTGCGCCTGAGCGAGGCGAAATACGCGATCGCTGTTAAAAGGACAATTACAAACAGGAATCGAATGCAACCGGCGCAGGAACACTGCCAGCGCATCAACAATATTTTCACCTGAATCAGGATATTCTTCTAATACCTGGAATGCTGTTTTCCCGGGGATCGCAGTGGTGAGTAACCATGCATCATCAGGAGTACGGATAAAATGCTTGATGGTCGGAAGAGGCATAAATTCCGTCAGCCAGTTTAGTCTGACCATCTCATCTGTAACATCATTGGCAACGCTACCTTTGCCATGTTTCAGAAACAACTCTGGCGCATCGGGCTTCCCATACAATCGATAGATTGTCGCACCTGATTGCCCGACATTATCGCGAGCCCATTTATACCCATATAAATCAGCATCCATGTTGGAATTTAATCGCGGCCTGGAGCAAGACGTTTCCCGTTGAATATGGCTCATAACACCCCTTGTATTACTGTTTATGTAAGCAGACAGTTTTATTGTTCATGATGATATATTTTTATCTTGTGCAATGTAACATCAGAGATTTTGAGACACAACGTGGCTTTGTTGAATAAATCGAACTTTTGCTGAGTTGAAGGATCAGAGCCTACGTTCCGAATACGGTCAAAAAAAAGGCCATCCGTCAGGATGGCCTTCGCATTAATATGCCGCTTCGAATTCTTTTAGGAAGCGTGTATCGTTTTCAGAGAACATACGGAGGTCTTTCACCTGATATTTCAGGTTTGTGATACGCTCGATACCCATACCGAGTCCATAACCGCTGTATATTTTGCTGTCTATACCATTTGATTCAAGTACGTTCGGGTCTACCATACCGCAACCGAGGATTTCTACCCAGCCGGTGTGTTTACAGAACGGACATCCTTTACCGCCGCAGATATTACAGCTGATATCCATTTCCGCACTTGGTTCAGCAAACGGGAAGTAAGACGGACGCAGACGGATCTTTGTATCAGCACCGAACATTTCTTTGGCAAAGAGCAGCAATACCTGCTTCAAGTCGGTGAATGATACGTTTTTATCTACATACAGCGCTTCTACCTGATGGAAGAAACAGTGTGCGCGATAGCTGATAGCTTCGTTACGATATACACGTCCCGGACAGATGATGCGGATAGGAGGCTGTGAAGTTTCCATCACACGAGTCTGTACAGAAGAAGTATGTGTACGCAATACTACGTCCGGGTGAGCTTCGATAAAGAAAGTGTCCTGCATATCGCGTGCCGGATGATCTTCGGCAAAGTTCAGTGCCGAGAACACGTGCCAGTCATCTTCAATTTCCGGACCTTCGGCAATGCTGAATCCCAGACGGGCAAAGATATCAATGATTTCGTTCTTTACAATGGTGAGCGGGTGGCGTGTACCGAGTTCTACAGGATAAGCCGAACGCGTCAAATCCAGTCCGTCACAATCGTTGTCCTGACTTTCAAACATTTCTTTCAGCGCGTTGATTTTGTCCTGCGCTTTTGTTTTCAGTTCATTCAGTCTCATGCCGACTTCTTTTTTCTGTTCGGCAGCTACATTACGGAAATCTGCCATTAAGTCGTTAATGGCTCCCTTCTTACTTAGGTATTTGATGCGGAGAGCTTCGAGTTCTTCGGCATTGGAGGCGTGTAAGGCTTCCACCTCTTTCAGAAGTTGTTCAATCTTAGCTATCATTTTTTAATATTTTTAGCGGCCCCGTTAAACAAAATTATTTGTAGAGGCTGTTTCGTCCTCACGGACTCATCAGACCGGAAAGCACATCCGGTGACAGCTCAGGCTACTTTGTTTCTTTCGACACTGCAAATATAAGAACATTATTTGAAAGTTCAAGTGAAACTTTAAATTTTAACAATAGATTAACCATTGCAAACAAAACAAAAAAAAGGTAGCCCAATTGTAAAACGAAAGGCCCAGTCTTTCGACTGAGCCTTTCGTTTTATCCTACAGTCGCTCGGCGATCGAAGGCTTCGGAAAAAAAAGGCCATCCGTCAGGATGGCCTTCGCATTAATATGCCGCTTCGAATTCTTTTAGGAAGCGTGTATCGTTTTCAGAGAACATACGGAGGTCTTTCACCTGATATTTCAGGTTTGTGATACGCTCGATACCCATACCGAGTCCATAACCGCTGTATATTTTGCTGTCTATACCATTTGATTCAAGTACGTTCGGGTCTACCATACCGCAACCGAGGATTTCTACCCAGCCGGTGTGTTTACAGAACGGACATCCTTTACCGCCGCAGATATTACAGCTGATATCCATTTCCGCACTTGGTTCAGCAAACGGGAAGTAAGACGGACGCAGACGGATCTTTGTATCAGCACCGAACATTTCTTTGGCAAAGAGCAGCAATACCTGCTTCAAGTCGGTGAATGATACGTTTTTATCTACATACAGCGCTTCTACCTGATGGAAGAAACAGTGTGCGCGATAGCTGATAGCTTCGTTACGATATACACGTCCCGGACAGATGATGCGGATAGGAGGCTGTGAAGTTTCCATCACACGAGTCTGTACAGAAGAAGTATGTGTACGCAATACTACGTCCGGGTGAGCTTCGATAAAGAAAGTGTCCTGCATATCGCGTGCCGGATGATCTTCGGCAAAGTTCAGTGCCGAGAACACGTGCCAGTCATCTTCAATTTCCGGACCTTCGGCAATGCTGAATCCCAGACGGGCAAAGATATCAATGATTTCGTTCTTTACAATGGTGAGCGGGTGGCGTGTACCGAGTTCTACAGGATAAGCCGAACGCGTCAAATCCAGTCCGTCACAATCGTTGTCCTGACTTTCAAACATTTCTTTCAGCGCGTTGATTTTGTCCTGCGCTTTTGTTTTCAGTTCATTCAGTCTCATGCCGACTTCTTTTTTCTGTTCGGCAGCTACATTACGGAAATCTGCCATTAAGTCGTTAATGGCTCCCTTCTTACTTAGGTATTTGATGCGGAGAGCTTCGAGTTCTTCGGCATTGGAGGCGTGTAAGGCTTCCACCTCTTTCAGAAGTTGTTCAATCTTAGCTATCATTTTTTAATATTTTTAGCGGCCCCGTTAAACAAAATTATTTGTAGAGGCTGTTTCGTCCTCACGGACTCATCAGACCGGAAAGCACATCCGGTGACAGCTCAGGCTACTTTGTTTCTTTCGACACTGCAAATATAAGAACATTATTTGAAAGTTCAAGTGAAACTTTAAATTTTAACAATAGATTAACCATTGCAAACAAAACAAAAAAAAGGTAGCCCAATTGTAAAACGAAAGGCCCAGTCTTTCGACTGAGCCTTTCGTTTTATCCTAGGATCAGCTGTACGTACTCGCAGTTCAACCTGTTGATAGTACGTACTAAGCTCTCATGTTTCACGTACTAAGCTCTCATGTTTAACGTACTAAGCTCTCATGTTTAACGAACTAAACCCTCATGGCTAACGTACTAAGCTCTCATGGCTAACGTACTAAGCTCTCATGTTTCACGTACTAAGCTCTCATGTTTGAACAATAAAATTAATATAAATCAGCAACTTAAATAGCCTCTAAGGTTTTAAGTTTTATAAGAAAAAAAAGAATATATAAGGCTTTTAAAGCTTTTAAGGTTTAACGGTTGTGGACAACAAGCCAGGGATGTAACGCACTGAGAAGCCCTTAGAGCCTCTCAAAGCAATTTTGAGTGACACAGGAACACTTAACGGCTGACATGGGGCGGCCGCGTAG
SEQ ID NO:90(用于基因组整合进普通拟杆菌基因组中的编码草酸盐降解途径的质粒)：
GGAGAGCTGTATGTGGGGTGGAAGAAGCTATCGTAAAAATGCTGGAGCAGCTTGATCCTCATTCTACCTATTCTGATCCGGAGGAAGTGAAGAGAATGAACGAGCCGTTGCAGGGAAACTTCGAAGGTATCGGTATACAGTTTAATATGGCGGAGGACACTTTGCTGGTGATACAGCCTGTATCGGGCGGACCGTCCGAAAAAGTCGGTATTCTGGCCGGTGATCGTATCGTCATGGTGGAAGATACGCTGATAGCCGGTGTAAAGATGAGTACCGAGGATATTATGCGTCGCCTCCGTGGTCCTAAAGATTCAAAAGTGAATCTGAAAATACTTCGCCGAGGGGTGAAGGAACTGCTCCCCTTTACGGTGAAAAGGGATAAAATACCTGTTTACAGCTTGGATGCTTCCTATATGATTAAAGATAAGATCGGTTATATTCGTATCAACCGTTTTGCCGCCACTACCCATGAGGAGTTCAAGAAAGCGCTGACCGGGTTGCAGAAGAAAGGAATGAAAGACTTGATTCTTGATTTGCAGGGCAATGGCGGGGGGTATTTGAATGCTGCTATCGATATTGCCAATGAATTTTTGGGTAAAAAGGAACTGATTGTCTATACGGAGGGCCGTCGTAATCCTCGTAGCGAGTTTTTTGCCAAAGGTACGGGCGAGTTTCAGAACGGTCGTCTGATGGTATTGGTTGATGAATTTTCGGCTTCTGCCAGTGAGATTGTGACCGGAGCTGTTCAGGATTGGGACAGAGGCGTTGTGGTAGGCCGTCGCAGTTTTGGTAAAGGCTTGGTGCAGCGTCCCATTGATTTGCCGGACGGTTCGATGATCCGTCTGACGGTGGCCCGCTATTATACGCCTGCCGGACGTTGTATCCAGAAACCTTACGAGAGCATTGAAAAGTATAATGAAGACCTGATAGACCGTTATAATAAAGGAGAAATGATGAGTGAAGACAGCATCCACTTTCCGGATTCACTGAAGTTCAAGACTCATAGGTTGGCCCGTACGGTATATGGTGGCGGAGGGATCATGCCCGATTATTTTGTGCCGATTGATACGACTCTCTATACTAAATACCATCGTCAGTTGCGTGATAAAGGAGCATTGATGAAAGCTCATTTCCATTTTATTGATGCCCATCGTAAAGAATGGCTGGGCAAATACAAGACTTTCAATGAGTTCTACAAACGTTTTGAAGTGACTCCGGATATGTTGACACAACTGGTTGCTACCGGCAAGGAGATGGGAGTGGAATATAATGAAGAAGAGTATCAAAAGGCCCTGCCTCTGCTGAGGTTGCAGATGAAAGCATTGATAGCCCGTGATTTATGGGATATGAATGAATATTATCACGTCATTAATGACGCGAATGAAAGTATCAGGAAAGCGCTGGAACTGTTGGAGCAACCGGACTTTGAGGGGCTGCTGTTGAAAAAGCGATAGTCAAGACTTTATATTAACTGCAGGGGCGCATAGTTGCACTCCTGCAGTTGTCTGAAAAACTTGTAGGATAAAACGAAAGGCTCAGTCGAAAGACTGGGCCTTTCGTTTTACAATTGGGCTACCTTTTTTTTGTTTTGTTTGCAATGGTTAATCTATTGTTAAAATTTAAAGTTTCACTTGAACTTTCAAATAATGTTCTTATATTTGCAGTGTCGAAAGAAACAAAGTAGCCTGGCATCTTAATCAATTTTAAACTCACCAAATCAAAATATGAAAAAAAAAAATAATAAAATGTCAACTCCACTTCAAGGAATTAAAGTTCTCGATTTCACCGGTGTGCAATCTGGCCCATCTTGTACTCAAATGCTGGCCTGGTTTGGCGCTGACGTTATTAAAATTGAACGTCCCGGCGTTGGTGACGTAACGCGTCACCAGCTGCGAGATATTCCTGATATCGATGCGCTTTACTTCACCATGCTTAACAGTAACAAACGTTCTATTGAGTTAAATACCAAAACAGCGGAAGGCAAAGAGGTAATGGAAAAGCTGATCCGCGAAGCTGATATCTTAGTCGAGAACTTTCATCCAGGGGCCATTGATCACATGGGCTTCACCTGGGAGCATATTCAAGAAATCAATCCACGTCTGATTTTTGGTTCGATCAAAGGGTTTGATGAGTGTTCGCCTTATGTGAATGTAAAAGCCTATGAAAACGTTGCTCAGGCAGCGGGTGGCGCGGCATCCACTACGGGTTTTTGGGATGGTCCGCCGCTGGTAAGCGCTGCAGCGTTGGGTGACAGCAACACCGGAATGCATTTGCTGATCGGTTTACTTGCTGCTTTGCTGCATCGCGAAAAAACGGGGCGTGGGCAACGAGTCACCATGTCAATGCAGGATGCCGTATTGAACCTTTGCCGCGTGAAATTACGTGACCAGCAGCGTCTAGATAAATTGGGTTATCTGGAAGAATACCCGCAGTATCCGAATGGTACATTTGGTGATGCAGTTCCCCGCGGTGGTAATGCAGGTGGTGGCGGTCAGCCTGGCTGGATCCTGAAATGTAAAGGCTGGGAAACCGATCCTAACGCCTATATTTATTTCACTATTCAGGAGCAAAACTGGGAAAACACCTGTAAAGCCATCGGCAAACCAGAATGGATTACCGATCCGGCATACAGTACAGCCCATGCACGACAGCCACATATTTTCGATATTTTTGCTGAAATCGAAAAATACACTGTCACTATTGATAAACATGAAGCGGTGGCCTATTTGACTCAGTTTGATATTCCTTGTGCACCGGTTTTAAGTATGAAAGAAATTTCACTTGATCCCTCTTTGCGCCAAAGTGGCAGTGTTGTTGAAGTGGAACAACCGTTGCGTGGAAAATATCTGACCGTTGGTTGTCCAATGAAATTCTCTGCCTTTACGCCGGATATTAAAGCTGCGCCGCTATTAGGTGAACATACCGCTGCTGTATTGCAGGAGCTGGGTTATAGCGACGATGAAATTGCTGCAATGAAGCAAAACCACGCCATCTGAGGTTCCTAACCTGCCTGGCATCAACTACCTAAATTAATTATCACCCCAAATTAATAACATAAAAATATAATAAAATGAGTAACGACGACAATGTAGAGTTGACTGATGGCTTTCATGTTTTGATCGATGCCCTGAAAATGAATGACATCGATACCATGTATGGTGTTGTCGGCATTCCTATCACGAACCTGGCTCGTATGTGGCAAGATGACGGTCAGCGTTTTTACAGCTTCCGTCACGAACAACACGCAGGTTATGCAGCTTCTATCGCCGGTTACATCGAAGGAAAACCTGGCGTTTGCTTGACCGTTTCCGCCCCTGGCTTCCTGAACGGCGTGACTTCCCTGGCTCATGCAACCACCAACTGCTTCCCAATGATCCTGTTGAGCGGTTCCAGTGAACGTGAAATCGTCGATTTGCAACAGGGCGATTACGAAGAAATGGATCAGATGAATGTTGCACGTCCACACTGCAAAGCTTCTTTCCGTATCAACAGCATCAAAGACATTCCAATCGGTATCGCTCGTGCAGTTCGCACCGCTGTATCCGGACGTCCAGGTGGTGTTTACGTTGACTTGCCAGCAAAACTGTTCGGTCAGACCATTTCTGTAGAAGAAGCTAACAAACTGCTCTTCAAACCAATCGATCCAGCTCCGGCACAGATTCCTGCTGAAGATGCTATCGCTCGCGCTGCTGACCTGATCAAGAACGCCAAACGTCCAGTTATCATGCTGGGTAAAGGCGCTGCATACGCACAATGCGACGACGAAATCCGCGCACTGGTTGAAGAAACCGGCATCCCATTCCTGCCAATGGGTATGGCTAAAGGCCTGCTGCCTGACAACCATCCACAATCCGCTGCTGCAACCCGTGCTTTCGCACTGGCACAGTGTGACGTTTGCGTACTGATCGGCGCTCGTCTGAACTGGCTGATGCAGCACGGTAAAGGCAAAACCTGGGGCGACGAACTGAAGAAATACGTTCAGATCGACATCCAGGCTAACGAAATGGACAGCAACCAGCCTATCGCTGCACCAGTTGTTGGTGACATCAAGTCCGCCGTTTCCCTGCTCCGCAAAGCACTGAAAGGCGCTCCAAAAGCTGACGCTGAATGGACCGGCGCTCTGAAAGCCAAAGTTGACGGCAACAAAGCCAAACTGGCTGGCAAGATGACTGCCGAAACCCCATCCGGAATGATGAACTACTCCAATTCCCTGGGCGTTGTTCGTGACTTCATGCTGGCAAATCCGGATATTTCCCTGGTTAACGAAGGCGCTAATGCACTCGACAACACTCGTATGATTGTTGACATGCTGAAACCACGCAAACGTCTTGACTCCGGTACCTGGGGTGTTATGGGTATTGGTATGGGCTACTGCGTTGCTGCAGCTGCTGTTACCGGCAAACCGGTTATCGCTGTTGAAGGCGATAGCGCATTCGGTTTCTCCGGTATGGAACTGGAAACCATCTGCCGTTACAACCTGCCAGTTACCGTTATCATCATGAACAATGGTGGTATCTATAAAGGTAACGAAGCAGATCCACAACCAGGCGTTATCTCCTGTACCCGTCTGACCCGTGGTCGTTACGACATGATGATGGAAGCATTTGGCGGTAAAGGTTATGTTGCCAATACTCCAGCAGAACTGAAAGCTGCTCTGGAAGAAGCTGTTGCTTCCGGCAAACCATGCCTGATCAACGCGATGATCGATCCAGACGCTGGTGTCGAATCTGGCCGTATCAAGAGCCTGAACGTTGTAAGTAAAGTTGGCAAGAAATAAGGTTCCTAAACGACCTGGCATCAACTACCTACATTAATAACCAACCCAAATGCCTAATCAATAAATATTATAAAATGAATAATCCACAAACAGGACAATCAACAGGCCTCTTGGGCAATCGTTGGTTCTACTTGGTATTAGCAGTTTTGCTGATGTGTATGATCTCGGGTGTCCAATATTCCTGGACACTGTACGCTAACCCGGTTAAAGACAACCTTGGCGTTTCTTTGGCTGCGGTTCAGACGGCTTTCACACTCTCTCAGGTCATTCAAGCTGGTTCTCAGCCTGGTGGTGGTTACTTCGTTGATAAATTCGGTCCAAGAATTCCATTGATGTTCGGTGGTGCGATGGTTCTCGCTGGCTGGACCTTCATGGGTATGGTTGACAGTGTTCCTGCTCTGTATGCTCTTTATACTCTGGCCGGTGCAGGTGTTGGTATCGTTTACGGTATCGCGATGAACACGGCTAACAGATGGTTCCCGGACAAACGCGGTCTGGCTTCCGGTTTCACCGCTGCCGGTTACGGTCTGGGTGTTCTGCCGTTCCTGCCACTGATCAGCTCCGTTCTGAAAGTTGAAGGTGTTGGCGCAGCATTCATGTACACCGGTTTGATCATGGGTATCCTGATTATCCTGATCGCTTTCGTTATCCGTTTCCCTGGCCAGCAAGGCGCCAAAAAACAAATCGTTGTTACCGACAAGGATTTCAATTCTGGCGAAATGCTGAGAACACCACAATTCTGGGTTCTGTGGACCGCATTCTTTTCCGTTAACTTTGGTGGTTTGCTGCTGGTTGCCAACAGCGTCCCTTACGGTCGCAGCCTCGGTCTTGCCGCAGGTGTGCTGACGATCGGTGTTTCGATCCAGAACCTGTTCAATGGTGGTTGCCGTCCTTTCTGGGGTTTCGTTTCCGATAAAATCGGCCGTTACAAAACCATGTCCGTCGTTTTCGGTATCAATGCTGTTGTTCTCGCACTTTTCCCGACGATTGCTGCCTTGGGCGATGTAGCCTTTATCGCCATGTTGGCAATCGCATTCTTCACATGGGGTGGTAGCTACGCTCTGTTCCCATCGACCAACAGCGATATTTTCGGTACGGCATACTCTGCCAGAAACTATGGTTTCTTCTGGGCTGCAAAAGCAACTGCCTCGATCTTCGGTGGTGGTCTGGGTGCTGCAATTGCAACCAACTTCGGATGGAATACCGCTTTCCTGATTACTGCGATTACTTCTTTCATCGCATTTGCTCTGGCTACCTTCGTTATTCCAAGAATGGGCCGTCCAGTCAAGAAAATGGTCAAATTGTCTCCAGAAGAAAAAGCTGTACATTAAGGTTCCTAATTCCCCTGGCATCAACTACCTAAATTACTTATCAAACCACTTAGTAGTAAAAAAAATATAACAAAATGACAAATAATGAAAGCAAAGGGCCGTTTGAAGGCTTATTAGTTATCGATATGACACATGTCCTTAATGGACCTTTCGGAACTCAACTTCTTTGTAATATGGGCGCAAGGGTAATTAAAGTTGAGCCGCCGGGTCATGGTGATGATACCCGCACATTTGGTCCCTATGTGGATGGACAGTCACTCTATTACAGTTTTATTAATCATGGCAAAGAGAGTGTGGTTCTTGATTTAAAGAATGATCACGATAAAAGTATATTTATAAATATGCTCAAACAAGCTGATGTATTAGCTGAGAATTTTCGCCCAGGTACAATGGAAAAACTGGGGTTTTCATGGGAAACGCTTCAAGAAATCAACCCGCGCCTCATATATGCTTCATCGTCAGGTTTCGGACATACCGGTCCGCTAAAAGATGCTCCTGCCTACGATACCATCATTCAGGCAATGAGCGGGATAATGATGGAAACAGGATATCCTGATGCTCCGCCAGTGCGCGTTGGTACATCTCTTGCGGATCTATGCGGCGGTGTCTATTTATTCAGCGGAATAGTGAGTGCACTTTATGGCCGCGAAAAGAGCCAGAGAGGGGCGCATGTCGATATAGCGATGTTTGATGCCACGCTGAGTTTTCTGGAGCATGGTCTGATGGCATATATCGCAACTGGGAAGTCACCACAACGTCTGGGAAATCGCCATCCCTACATGGCACCTTTTGATGTTTTCAATACTCAGGATAAGCCGATTACGATTTGTTGTGGTAATGACAAGCTTTTTTCTGCGTTATGCCAGGCACTGGAGCTTACGGAACTGGTTAATGATCCCCGATTTAGCAGCAATATTTTACGCGTACAAAACCAGGCTATTCTTAAACAATATATTGAGCGGACGTTAAAAACGCAGGCAGCTGAAGTTTGGTTAGCCAGAATACATGAAGTTGGTGTACCCGTCGCGCCGTTATTAAGTGTGGCTGAGGCCATTAAATTGCCACAAACTCAGGCGAGAAATATGTTGATTGAAGCCGGGGGAATAATGATGCCGGGTAATCCGATAAAAATCAGCGGCTGCGCGGACCCGCATGTTATGCCGGGAGCGGCAACGCTCGACCAGCATGGGGAACAAATTCGCCAGGAGTTCTCATCATAAGGTTCCTAAAAACCCTGGCATCTTTTCCATAACAAAAATTAAACCTCCTCCATATGAAAAATAAAAATTATAAAATGACAAGTGCCGCTACTGTTACTGCTTCGTTCAACGATACTTTTAGCGTATCCGATAATGTCGCCGTTATTGTTCCTGAAACGGACACTCAGGTGACCTACAGGGATCTATCCCACATGGTGGGTCACTTCCAGACCATGTTCACAAATCCTAATTCTCCATTGTACGGAGCTGTTTTCAGACAAGATACAGTGGCGATATCCATGCGTAATGGGCTGGAATTTATCGTCGCTTTCCTCGGTGCTACTATGGACGCTAAAATTGGCGCGCCCTTGAATCCCAATTATAAGGAAAAGGAGTTCAATTTTTATTTGAATGACCTGAAATCTAAGGCGATTTGCGTCCCAAAGGGTACCACAAAGTTACAGAGTTCTGAAATTCTAAAATCTGCCTCCACGTTTGGATGTTTTATCGTAGAGCTGGCCTTCGATGCGACCAGGTTTAGGGTAGAGTATGATATATACTCTCCAGAGGACAACTACAAAAGGGTTATTTACCGGTCTTTGAACAACGCCAAATTTGTCAACACAAATCCCGTTAAATTCCCTGGGTTTGCCCGTTCCAGTGACGTTGCCCTGATTTTGCATACCAGTGGTACCACCTCCACTCCAAAAACGGTGCCTTTGTTACATTTGAACATTGTGAGAAGCACGTTGAACATTGCTAACACTTACAAGCTAACGCCCTTGGACAGATCTTATGTCGTGATGCCTCTTTTCCACGTCCATGGGTTAATTGGTGTTTTACTTTCCACTTTTAGAACTCAGGGTTCTGTTGTGGTTCCCGATGGATTCCATCCAAAGTTATTCTGGGACCAATTTGTTAAGTACAACTGTAATTGGTTCAGTTGCGTTCCCACAATAAGCATGATTATGCTGAACATGCCCAAACCAAACCCTTTCCCACACATTAGATTCATCAGATCGTGTTCTTCTGCTTTGGCTCCAGCAACGTTCCATAAGCTGGAGAAGGAATTCAATGCACCTGTCTTGGAGGCCTATGCGATGACCGAAGCATCACATCAAATGACCTCAAACAATCTGCCTCCAGGAAAGAGAAAGCCTGGTACTGTGGGCCAGCCACAAGGAGTCACCGTCGTCATTCTAGATGACAATGACAATGTCTTGCCCCCGGGCAAAGTCGGCGAAGTTTCCATCAGAGGCGAAAACGTCACTTTGGGGTATGCTAATAATCCAAAAGCTAACAAGGAGAACTTCACCAAGAGAGAGAACTATTTCAGAACCGGTGACCAAGGTTATTTCGACCCTGAGGGGTTTTTGGTCCTTACAGGCAGAATCAAAGAGCTTATCAACAGGGGTGGTGAAAAGATTTCACCCATTGAGCTCGACGGCATTATGCTATCGCATCCAAAGATCGATGAAGCCGTTGCATTTGGTGTTCCCGACGATATGTACGGCCAAGTAGTTCAAGCCGCCATTGTTTTGAAGAAGGGAGAAAAAATGACCTACGAAGAACTGGTGAACTTCTTAAAGAAGCACCTAGCCTCTTTCAAAATTCCAACCAAGGTGTACTTTGTTGATAAGCTACCAAAAACCGCTACAGGTAAAATCCAGAGAAGAGTTATCGCAGAAACTTTTGCTAAGAGCAGCAGAAATAAGAGTAAGTTGTAGGGTTCCTAGCTGATTAGAAGGCCATCCTGACGGATGGCCTTTTTTTTGACTCTGTTGTGTCTTCTTTTAGGTGTGGAATAATTTTCCAACCATCACTTTATCTGTCTCCCGAACAGCACCTTATTCAGCCATCGGTTCACATACACGTTCACGAAAGCACATCCTACTCCGATAGCGGCTCCTGCCACTACATCACTGGGATAGTGCACCCCTTGGTTCATCCGGGCAAACCCTACTCCGCAAGCCCATACGGCTGAAGGGGCGATAACATACCACTTAGGATAAGTGATGGAGAGTGAGGTGGCTAAAGAAAAAGCTGCCGCAGTGTGTCCTGAAGGGAATGACGGACTATCGGCATCCGGTGCTCCGTATGCATGAATCTTATCCGGATACTTTACAAAAGGCCGGTCACGACCTATTATATGTTTGGCGGCGTATGTAATCCCCACTGCTTCAATCACACTGGTTCCTATGTAAATGGCATCTTTCAGCATCGGCTGGTCTTTCTTTATCAATGCATAGAGTCCCATGGCTGTAGGTATTCCTACGGGTAGAATCACTCCGCTAGCGGACAATCCGCGGCTCAGTCCGTGTACATCCCATCGGTTGATGGTTTTCAACGTGTTGATGTCTATATTCTGTGCGCTGAGGCTGAATGTAAAACAGGCTGTCAGTATAAGAAAAATAATCCTTTGGCGTTTCATTTAGTTTGGCATTTGTTCAGTGCAAAGATAGGAAAATAATGTAATTCGCATTTACCCTTCCCCGAATTAAAGAATAATTTCCATAATTCCTTGTATTATCGCTAGAATTCCTTTACTTTTGCATCGTGAATACACAGCTTTTAACAGAATTTTTAGTTCTAACAATTAAATAATTTAAATTCAATCATTTATGTGGTTAAGTAATTCATCTATTGGAAGGAAAGTGGTGATGAGTGTCACAGGTATCGCCCTTGTCCTGTTTCTGACGTTTCACATGGCGATGAACCTTGTTGCGCTCTTCTCGGAAGATGCTTACAACATGATTTGTGAATTCTTGGGAGCTAACTGGTACGCGTTGGTTGCAACTGCCGGGCTGGCTGCTCTGTTTGTAATTCACATCATCTACGCTTTCTGGTTGACCATGCAGAACCGTGCAGCTCGCGGTAGCGAACGTTATGCAGTGAACGTTAAACCTAAAACTGTGGAATGGGCATCTCAGAACATGCTTGTCCTGGGTATTGTCGTTATTGCCGGATTGGCTCTTCATTTTGCCAATTTCTGGTACAAAATGCAGTTTGCCGAAATTATCGGTGTTCATGATTTGGGCGCATTCGGTCCTACAGACGGTGCCGCTTACATTCGTGAAACATTCGGATGCCCTGTATTTACAGTGCTTTACCTCATTTGGTTAGCAGCTTTGTGGTTCCACCTGACTCACGGATTCTGGAGTGCTTTGCAGACTTTGGGCTGGAGCAATAAAATCTGGTTCGAGCGTTGGAAGACTATTGGTAACATCTACTCTACTTTGGTAGTTCTTGGTTTTGCAGCAGTAGTCGTTATCTACTTCGCTATGACTGGCGCGCCTGATAGGTGGGCTGCCCTTCCTGGTTGGCTTGGTTTCATCAGCCATCCGCTTGCCCTCATCTGTTACGCCGGCGGTAGCCGGCCAGCCTCGCAGAGCAGGATTCCCGTTGAGCACCGCCAGGTGCGAATAAGGGACAGTGAAGAAGGAACACCCGCTCGCGGGTGGGCCTACTTCACCTATCCTGCCCGGCTGACGCCGTTGGATACACCAAGGAAAGTCTACACGAACCCTTTGGCAAAATCCTGTATATCGTGCGAAAAAGGATGGATATACCGAAAAAATCGCTATAATGACCCCGAAGCAGGGTTATGCAGCGGAAAAGCGGGATTAAAAGTCGGGGATTGGTGAACAAAAAGGTGTTTCTCTCTTTAAGAGAAATATCGTTTTGCTAAACAGTTGATATTGAGGTATCATTTTATCGTAAAAGACATTTTTGCTCAACAATTGCTTGACGGAAATCAACAAATTTTAGCATTTTGTAAAAAAGTCGCTATATAATTTGGTGAATTGGAGTTATTTTCATATTTTTGCATCCCGAAGAGTTTCTCTTAAAGAGAGAAACATCTTTTGCATACCTTTTCCGACCGAATTTTTATGTCGTAAAGAGGGGCTTTGCAGGGGGTGGACTCAGAAAGATGAGAATAGATGACTATTGTAGTTGAAACACATAGAAAGTTGCTGATATACAGACCGATACGCATATCGGGATGAACCATGAGTACGTTCTTTTCTCAAAAAACATAAATATTCGAAAAGAGATGCAATAAATTAAGGAGAGGTTATAATGAACAAAGTAAATATAAAAGATAGTCAAAATTTTATTACTTCAAAATATCACATAGAAAAAATAATGAATTGCATAAGTTTAGATGAAAAAGATAACATCTTTGAAATAGGTGCAGGGAAAGGTCATTTTACTGCTGGATTGGTAAAGAGATGTAATTTTGTAACGGCGATAGAAATTGATTCTAAATTATGTGAGGTAACTCGTAATAAGCTCTTAAATTATCCTAACTATCAAATAGTAAATGATGATATACTGAAATTTACATTTCCTAGCCACAATCCATATAAAATATTTGGCAGCATACCTTACAACATAAGCACAAATATAATTCGAAAAATTGTTTTTGAAAGTTCAGCCACAATAAGTTATTTAATAGTGGAATATGGTTTTGCTAAAATGTTATTAGATACAAACAGATCACTAGCATTGCTGTTAATGGCAGAGGTAGATATTTCTATATTAGCAAAAATTCCTAGGTATTATTTCCATCCAAAACCTAAAGTGGATAGCACATTAATTGTATTAAAAAGAAAGCCAGCAAAAATGGCATTTAAAGAGAGAAAAAAATATGAAACTTTTGTAATGAAATGGGTTAACAAAGAGTACGAAAAACTGTTTACAAAAAATCAATTTAATAAAGCTTTAAAACATGCGAGAATATATGATATAAACAATATTAGTTTCGAACAATTTGTATCGCTATTTAATAGTTATAAAATATTTAACGGCTAAAAACAATAGGCCACATGCAACTGTAAATGTTTACGCGGGTACCGACACCGCGGTGGAGGGGAATTGTGTTACAACCAATTAACCAATTCTGATTAGAAAAACTCATCGAGCATCAAATGAAACTGCAATTTATTCATATCAGGATTATCAATACCATATTTTTGAAAAAGCCGTTTCTGTAATGAAGGAGAAAACTCACCGAGGCAGTTCCATAGGATGGCAAGATCCTGGTATCGGTCTGCGATTCCGACTCGTCCAACATCAATACAACCTATTAATTTCCCCTCGTCAAAAATAAGGTTATCAAGTGAGAAATCACCATGAGTGACGACTGAATCCGGTGAGAATGGCAAAAGCTTATGCATTTCTTTCCAGACTTGTTCAACAGGCCAGCCATTACGCTCGTCATCAAAATCACTCGCATCAACCAAACCGTTATTCATTCGTGATTGCGCCTGAGCGAGGCGAAATACGCGATCGCTGTTAAAAGGACAATTACAAACAGGAATCGAATGCAACCGGCGCAGGAACACTGCCAGCGCATCAACAATATTTTCACCTGAATCAGGATATTCTTCTAATACCTGGAATGCTGTTTTCCCGGGGATCGCAGTGGTGAGTAACCATGCATCATCAGGAGTACGGATAAAATGCTTGATGGTCGGAAGAGGCATAAATTCCGTCAGCCAGTTTAGTCTGACCATCTCATCTGTAACATCATTGGCAACGCTACCTTTGCCATGTTTCAGAAACAACTCTGGCGCATCGGGCTTCCCATACAATCGATAGATTGTCGCACCTGATTGCCCGACATTATCGCGAGCCCATTTATACCCATATAAATCAGCATCCATGTTGGAATTTAATCGCGGCCTGGAGCAAGACGTTTCCCGTTGAATATGGCTCATAACACCCCTTGTATTACTGTTTATGTAAGCAGACAGTTTTATTGTTCATGATGATATATTTTTATCTTGTGCAATGTAACATCAGAGATTTTGAGACACAACGTGGCTTTGTTGAATAAATCGAACTTTTGCTGAGTTGAAGGATCAGAGCCTACGTTCCGAATACGGTCAAAAAAAAGGCCATCCGTCAGGATGGCCTTCGCATTAATATGCCGCTTCGAATTCTTTTAGGAAGCGTGTATCGTTTTCAGAGAACATACGGAGGTCTTTCACCTGATATTTCAGGTTTGTGATACGCTCGATACCCATACCGAGTCCATAACCGCTGTATATTTTGCTGTCTATACCATTTGATTCAAGTACGTTCGGGTCTACCATACCGCAACCGAGGATTTCTACCCAGCCGGTGTGTTTACAGAACGGACATCCTTTACCGCCGCAGATATTACAGCTGATATCCATTTCCGCACTTGGTTCAGCAAACGGGAAGTAAGACGGACGCAGACGGATCTTTGTATCAGCACCGAACATTTCTTTGGCAAAGAGCAGCAATACCTGCTTCAAGTCGGTGAATGATACGTTTTTATCTACATACAGCGCTTCTACCTGATGGAAGAAACAGTGTGCGCGATAGCTGATAGCTTCGTTACGATATACACGTCCCGGACAGATGATGCGGATAGGAGGCTGTGAAGTTTCCATCACACGAGTCTGTACAGAAGAAGTATGTGTACGCAATACTACGTCCGGGTGAGCTTCGATAAAGAAAGTGTCCTGCATATCGCGTGCCGGATGATCTTCGGCAAAGTTCAGTGCCGAGAACACGTGCCAGTCATCTTCAATTTCCGGACCTTCGGCAATGCTGAATCCCAGACGGGCAAAGATATCAATGATTTCGTTCTTTACAATGGTGAGCGGGTGGCGTGTACCGAGTTCTACAGGATAAGCCGAACGCGTCAAATCCAGTCCGTCACAATCGTTGTCCTGACTTTCAAACATTTCTTTCAGCGCGTTGATTTTGTCCTGCGCTTTTGTTTTCAGTTCATTCAGTCTCATGCCGACTTCTTTTTTCTGTTCGGCAGCTACATTACGGAAATCTGCCATTAAGTCGTTAATGGCTCCCTTCTTACTTAGGTATTTGATGCGGAGAGCTTCGAGTTCTTCGGCATTGGAGGCGTGTAAGGCTTCCACCTCTTTCAGAAGTTGTTCAATCTTAGCTATCATTTTTTAATATTTTTAGCGGCCCCGTTAAACAAAATTATTTGTAGAGGCTGTTTCGTCCTCACGGACTCATCAGACCGGAAAGCACATCCGGTGACAGCTCAGGCTACTTTGTTTCTTTCGACACTGCAAATATAAGAACATTATTTGAAAGTTCAAGTGAAACTTTAAATTTTAACAATAGATTAACCATTGCAAACAAAACAAAAAAAAGGTAGCCCAATTGTAAAACGAAAGGCCCAGTCTTTCGACTGAGCCTTTCGTTTTATCCTACAGTCGCTCGGCGATCGAAGGCTTCGGAAAAAAAAGGCCATCCGTCAGGATGGCCTTCGCATTAATATGCCGCTTCGAATTCTTTTAGGAAGCGTGTATCGTTTTCAGAGAACATACGGAGGTCTTTCACCTGATATTTCAGGTTTGTGATACGCTCGATACCCATACCGAGTCCATAACCGCTGTATATTTTGCTGTCTATACCATTTGATTCAAGTACGTTCGGGTCTACCATACCGCAACCGAGGATTTCTACCCAGCCGGTGTGTTTACAGAACGGACATCCTTTACCGCCGCAGATATTACAGCTGATATCCATTTCCGCACTTGGTTCAGCAAACGGGAAGTAAGACGGACGCAGACGGATCTTTGTATCAGCACCGAACATTTCTTTGGCAAAGAGCAGCAATACCTGCTTCAAGTCGGTGAATGATACGTTTTTATCTACATACAGCGCTTCTACCTGATGGAAGAAACAGTGTGCGCGATAGCTGATAGCTTCGTTACGATATACACGTCCCGGACAGATGATGCGGATAGGAGGCTGTGAAGTTTCCATCACACGAGTCTGTACAGAAGAAGTATGTGTACGCAATACTACGTCCGGGTGAGCTTCGATAAAGAAAGTGTCCTGCATATCGCGTGCCGGATGATCTTCGGCAAAGTTCAGTGCCGAGAACACGTGCCAGTCATCTTCAATTTCCGGACCTTCGGCAATGCTGAATCCCAGACGGGCAAAGATATCAATGATTTCGTTCTTTACAATGGTGAGCGGGTGGCGTGTACCGAGTTCTACAGGATAAGCCGAACGCGTCAAATCCAGTCCGTCACAATCGTTGTCCTGACTTTCAAACATTTCTTTCAGCGCGTTGATTTTGTCCTGCGCTTTTGTTTTCAGTTCATTCAGTCTCATGCCGACTTCTTTTTTCTGTTCGGCAGCTACATTACGGAAATCTGCCATTAAGTCGTTAATGGCTCCCTTCTTACTTAGGTATTTGATGCGGAGAGCTTCGAGTTCTTCGGCATTGGAGGCGTGTAAGGCTTCCACCTCTTTCAGAAGTTGTTCAATCTTAGCTATCATTTTTTAATATTTTTAGCGGCCCCGTTAAACAAAATTATTTGTAGAGGCTGTTTCGTCCTCACGGACTCATCAGACCGGAAAGCACATCCGGTGACAGCTCAGGCTACTTTGTTTCTTTCGACACTGCAAATATAAGAACATTATTTGAAAGTTCAAGTGAAACTTTAAATTTTAACAATAGATTAACCATTGCAAACAAAACAAAAAAAAGGTAGCCCAATTGTAAAACGAAAGGCCCAGTCTTTCGACTGAGCCTTTCGTTTTATCCTAGGATCAGCTGTACGTACTCGCAGTTCAACCTGTTGATAGTACGTACTAAGCTCTCATGTTTCACGTACTAAGCTCTCATGTTTAACGTACTAAGCTCTCATGTTTAACGAACTAAACCCTCATGGCTAACGTACTAAGCTCTCATGGCTAACGTACTAAGCTCTCATGTTTCACGTACTAAGCTCTCATGTTTGAACAATAAAATTAATATAAATCAGCAACTTAAATAGCCTCTAAGGTTTTAAGTTTTATAAGAAAAAAAAGAATATATAAGGCTTTTAAAGCTTTTAAGGTTTAACGGTTGTGGACAACAAGCCAGGGATGTAACGCACTGAGAAGCCCTTAGAGCCTCTCAAAGCAATTTTGAGTGACACAGGAACACTTAACGGCTGACATGGGGCGGCCGCGTAG
SEQ ID NO:91(用于基因组整合进普通拟杆菌基因组中的编码草酸盐降解途径的质粒):
GGAGAGCTGTATGTGGGGTGGAAGAAGCTATCGTAAAAATGCTGGAGCAGCTTGATCCTCATTCTACCTATTCTGATCCGGAGGAAGTGAAGAGAATGAACGAGCCGTTGCAGGGAAACTTCGAAGGTATCGGTATACAGTTTAATATGGCGGAGGACACTTTGCTGGTGATACAGCCTGTATCGGGCGGACCGTCCGAAAAAGTCGGTATTCTGGCCGGTGATCGTATCGTCATGGTGGAAGATACGCTGATAGCCGGTGTAAAGATGAGTACCGAGGATATTATGCGTCGCCTCCGTGGTCCTAAAGATTCAAAAGTGAATCTGAAAATACTTCGCCGAGGGGTGAAGGAACTGCTCCCCTTTACGGTGAAAAGGGATAAAATACCTGTTTACAGCTTGGATGCTTCCTATATGATTAAAGATAAGATCGGTTATATTCGTATCAACCGTTTTGCCGCCACTACCCATGAGGAGTTCAAGAAAGCGCTGACCGGGTTGCAGAAGAAAGGAATGAAAGACTTGATTCTTGATTTGCAGGGCAATGGCGGGGGGTATTTGAATGCTGCTATCGATATTGCCAATGAATTTTTGGGTAAAAAGGAACTGATTGTCTATACGGAGGGCCGTCGTAATCCTCGTAGCGAGTTTTTTGCCAAAGGTACGGGCGAGTTTCAGAACGGTCGTCTGATGGTATTGGTTGATGAATTTTCGGCTTCTGCCAGTGAGATTGTGACCGGAGCTGTTCAGGATTGGGACAGAGGCGTTGTGGTAGGCCGTCGCAGTTTTGGTAAAGGCTTGGTGCAGCGTCCCATTGATTTGCCGGACGGTTCGATGATCCGTCTGACGGTGGCCCGCTATTATACGCCTGCCGGACGTTGTATCCAGAAACCTTACGAGAGCATTGAAAAGTATAATGAAGACCTGATAGACCGTTATAATAAAGGAGAAATGATGAGTGAAGACAGCATCCACTTTCCGGATTCACTGAAGTTCAAGACTCATAGGTTGGCCCGTACGGTATATGGTGGCGGAGGGATCATGCCCGATTATTTTGTGCCGATTGATACGACTCTCTATACTAAATACCATCGTCAGTTGCGTGATAAAGGAGCATTGATGAAAGCTCATTTCCATTTTATTGATGCCCATCGTAAAGAATGGCTGGGCAAATACAAGACTTTCAATGAGTTCTACAAACGTTTTGAAGTGACTCCGGATATGTTGACACAACTGGTTGCTACCGGCAAGGAGATGGGAGTGGAATATAATGAAGAAGAGTATCAAAAGGCCCTGCCTCTGCTGAGGTTGCAGATGAAAGCATTGATAGCCCGTGATTTATGGGATATGAATGAATATTATCACGTCATTAATGACGCGAATGAAAGTATCAGGAAAGCGCTGGAACTGTTGGAGCAACCGGACTTTGAGGGGCTGCTGTTGAAAAAGCGATAGTCAAGACTTTATATTAACTGCAGGGGCGCATAGTTGCACTCCTGCAGTTGTCTGAAAAACTTGTAGGATAAAACGAAAGGCTCAGTCGAAAGACTGGGCCTTTCGTTTTACAATTGGGCTACCTTTTTTTTGTTTTGTTTGCAATGGTTAATCTATTGTTAAAATTTAAAGTTTCACTTGAACTTTCAAATAATGTTCTTATATTTGCAGTGTCGAAAGAAACAAAGTAGCCTGGCATCACCTAAAAATTATAAATAAATTAATAAACTTTTATTAAAAAAAAATTACAAAATGAATAATCCACAAACAGGACAATCAACAGGCCTCTTGGGCAATCGTTGGTTCTACTTGGTATTAGCAGTTTTGCTGATGTGTATGATCTCGGGTGTCCAATATTCCTGGACACTGTACGCTAACCCGGTTAAAGACAACCTTGGCGTTTCTTTGGCTGCGGTTCAGACGGCTTTCACACTCTCTCAGGTCATTCAAGCTGGTTCTCAGCCTGGTGGTGGTTACTTCGTTGATAAATTCGGTCCAAGAATTCCATTGATGTTCGGTGGTGCGATGGTTCTCGCTGGCTGGACCTTCATGGGTATGGTTGACAGTGTTCCTGCTCTGTATGCTCTTTATACTCTGGCCGGTGCAGGTGTTGGTATCGTTTACGGTATCGCGATGAACACGGCTAACAGATGGTTCCCGGACAAACGCGGTCTGGCTTCCGGTTTCACCGCTGCCGGTTACGGTCTGGGTGTTCTGCCGTTCCTGCCACTGATCAGCTCCGTTCTGAAAGTTGAAGGTGTTGGCGCAGCATTCATGTACACCGGTTTGATCATGGGTATCCTGATTATCCTGATCGCTTTCGTTATCCGTTTCCCTGGCCAGCAAGGCGCCAAAAAACAAATCGTTGTTACCGACAAGGATTTCAATTCTGGCGAAATGCTGAGAACACCACAATTCTGGGTTCTGTGGACCGCATTCTTTTCCGTTAACTTTGGTGGTTTGCTGCTGGTTGCCAACAGCGTCCCTTACGGTCGCAGCCTCGGTCTTGCCGCAGGTGTGCTGACGATCGGTGTTTCGATCCAGAACCTGTTCAATGGTGGTTGCCGTCCTTTCTGGGGTTTCGTTTCCGATAAAATCGGCCGTTACAAAACCATGTCCGTCGTTTTCGGTATCAATGCTGTTGTTCTCGCACTTTTCCCGACGATTGCTGCCTTGGGCGATGTAGCCTTTATCGCCATGTTGGCAATCGCATTCTTCACATGGGGTGGTAGCTACGCTCTGTTCCCATCGACCAACAGCGATATTTTCGGTACGGCATACTCTGCCAGAAACTATGGTTTCTTCTGGGCTGCAAAAGCAACTGCCTCGATCTTCGGTGGTGGTCTGGGTGCTGCAATTGCAACCAACTTCGGATGGAATACCGCTTTCCTGATTACTGCGATTACTTCTTTCATCGCATTTGCTCTGGCTACCTTCGTTATTCCAAGAATGGGCCGTCCAGTCAAGAAAATGGTCAAATTGTCTCCAGAAGAAAAAGCTGTACATTAAGGTTCCTAACCTGCCTGGCATCTTAATCAATTTTAAACTCACCAAATCAAAATATGAAAAAAAAAAATAATAAAATGTCAACTCCACTTCAAGGAATTAAAGTTCTCGATTTCACCGGTGTGCAATCTGGCCCATCTTGTACTCAAATGCTGGCCTGGTTTGGCGCTGACGTTATTAAAATTGAACGTCCCGGCGTTGGTGACGTAACGCGTCACCAGCTGCGAGATATTCCTGATATCGATGCGCTTTACTTCACCATGCTTAACAGTAACAAACGTTCTATTGAGTTAAATACCAAAACAGCGGAAGGCAAAGAGGTAATGGAAAAGCTGATCCGCGAAGCTGATATCTTAGTCGAGAACTTTCATCCAGGGGCCATTGATCACATGGGCTTCACCTGGGAGCATATTCAAGAAATCAATCCACGTCTGATTTTTGGTTCGATCAAAGGGTTTGATGAGTGTTCGCCTTATGTGAATGTAAAAGCCTATGAAAACGTTGCTCAGGCAGCGGGTGGCGCGGCATCCACTACGGGTTTTTGGGATGGTCCGCCGCTGGTAAGCGCTGCAGCGTTGGGTGACAGCAACACCGGAATGCATTTGCTGATCGGTTTACTTGCTGCTTTGCTGCATCGCGAAAAAACGGGGCGTGGGCAACGAGTCACCATGTCAATGCAGGATGCCGTATTGAACCTTTGCCGCGTGAAATTACGTGACCAGCAGCGTCTAGATAAATTGGGTTATCTGGAAGAATACCCGCAGTATCCGAATGGTACATTTGGTGATGCAGTTCCCCGCGGTGGTAATGCAGGTGGTGGCGGTCAGCCTGGCTGGATCCTGAAATGTAAAGGCTGGGAAACCGATCCTAACGCCTATATTTATTTCACTATTCAGGAGCAAAACTGGGAAAACACCTGTAAAGCCATCGGCAAACCAGAATGGATTACCGATCCGGCATACAGTACAGCCCATGCACGACAGCCACATATTTTCGATATTTTTGCTGAAATCGAAAAATACACTGTCACTATTGATAAACATGAAGCGGTGGCCTATTTGACTCAGTTTGATATTCCTTGTGCACCGGTTTTAAGTATGAAAGAAATTTCACTTGATCCCTCTTTGCGCCAAAGTGGCAGTGTTGTTGAAGTGGAACAACCGTTGCGTGGAAAATATCTGACCGTTGGTTGTCCAATGAAATTCTCTGCCTTTACGCCGGATATTAAAGCTGCGCCGCTATTAGGTGAACATACCGCTGCTGTATTGCAGGAGCTGGGTTATAGCGACGATGAAATTGCTGCAATGAAGCAAAACCACGCCATCTGAGGTTCCTAAACGACCTGGCATCAACTACCTTTATTATTCAACAAACCAATTTAACATATTAAAAAAATAAAAAAATGACTACCACAGCAACGCAGCATTTTGCCCAGTTACTCCAGAAGTACGGAATTGATACTGTCTTCGGCATAGTAGGTATTCCGATCGTGCAGTTGGCCGACACTATGGTCGCGAATGGAATTAAGTTTATACCGTGTCGCAATGAGCAAGCCGCAAGTTACGCGGCAAGCGCCTACGGCTACATCTCGGACAAACCTGGTGTCCTGTTAATCGTGGGAGGGCCTGGCCTTATTCATGCCTTAGCGGGGATCTATAATAGTATGTCGAACCGCTGGCCCTTGTTGGTCATCGCCGGATCCAGCAGCCAGTCAGACATCCACAAGGGAGGTTTCCAGGAACTTGACCAGGTGTCCTTGCTAAGTCCGTTCCTAAAATTCACTGGTAAACTGACGCCGGATAACATCGATATGATAACCCAAAAAGCACTCAACTATTGCATTCAGGGAACCGCCGGAGTGAGCTATATCGATGTCCCCGCTGACTTCATCGAATACGAGAAGCCGCTTGAGGGCAATGATAGAACTGGTAACGAGCTGCCAATGATCCTGACGCCCAATATCTGCGGTCCAGACCCCAGTAAAATTAAGAAGGTGGTGCAATTGATACTCCAGCACAAGAACAAGAACATATTAATAGTGATAGGTAAAGGAGCAGTGAAGAATAGCCATGAGATTAGACGCTTGGTTAACACCTTTAACTTGCCGTTCCTTCCGACTCCCATGGCGAAAGGCATAGTGCCCGATAGCAGCCCTTTAAATGTGAGCAGCGCCCGGAGCCAGGCTCTTAAGATTGCTGACATCGTGTTAGTATTGGGAGCTCGCCTGAATTGGATCCTTCACTTCGGAACGAGTCCAAAGTGGAATAGCGAGAGTATTTTTATACAATTTGACAGTAACCCGGAAACGCTCGGAGACAATAACGTTAGTCCCGGAGCAGATTTAAGTATCTGGGGGGACATCGGACTATCGGTTACTGCACTGGTAGAAGAGCTCACGAGACAGGACAGTTGTTGGAAATACTCGGGAGTCAAACAGGAGATCAGGGAAAAGATACAGTTAAACCAGACCAGATTGCTCCGTAAGGAAAAAACGCGTGGAGCTCAGCTTAACTATAATCAAGTGTACGGCACCCTTCGTCCGTTAATTGACGACTATCGCACGATCTTGGTCACAGAAGGTGCCAACACAATGGACATCGCTAGAATAAGTTTTCCGACTGATGCACCTCGCAGAAGACTTGATGCTGGTACAAATGCTACAATGGGTATAGGTTTAGGTTACGCATTAGCGTGTAAAGCCAGCCACCCTGAGTTAGACGTTGTTTTAATCCAAGGCGACAGTGCCTTCGGTTTTAGCGCTATGGAGATCGAGACTGCCGTCCGTTGCCAATTAGCCCTTGTGATTGTCGTAATGAATAACTCTGGGATATATCACGGTGAGAAAGACATCGAAGGAGATCTGCCCCCGACCGCTTTGTCAAAAAATTGTAGATATGACTTGGTCGGTAAGGGTTTAGGAGCTAACGACTTCTTCGTCAATACCATCTCAGAGTTATCTCGCTGTTTTCAGCAGGCAGTTCAATTGAGCAGAACCAAGCGCGAAACCTCCGTCATTAATGTCATAATCGAACCGGGAGAGCAGAAACAGATCGCGTTCGCATGGCAAAACAAGCCTAGACTGTAAGGTTCCTAATTCCCCTGGCATCAACTACCTAAATTACTTATCAAACCACTTAGTAGTAAAAAAAATATAACAAAATGACAAATAATGAAAGCAAAGGGCCGTTTGAAGGCTTATTAGTTATCGATATGACACATGTCCTTAATGGACCTTTCGGAACTCAACTTCTTTGTAATATGGGCGCAAGGGTAATTAAAGTTGAGCCGCCGGGTCATGGTGATGATACCCGCACATTTGGTCCCTATGTGGATGGACAGTCACTCTATTACAGTTTTATTAATCATGGCAAAGAGAGTGTGGTTCTTGATTTAAAGAATGATCACGATAAAAGTATATTTATAAATATGCTCAAACAAGCTGATGTATTAGCTGAGAATTTTCGCCCAGGTACAATGGAAAAACTGGGGTTTTCATGGGAAACGCTTCAAGAAATCAACCCGCGCCTCATATATGCTTCATCGTCAGGTTTCGGACATACCGGTCCGCTAAAAGATGCTCCTGCCTACGATACCATCATTCAGGCAATGAGCGGGATAATGATGGAAACAGGATATCCTGATGCTCCGCCAGTGCGCGTTGGTACATCTCTTGCGGATCTATGCGGCGGTGTCTATTTATTCAGCGGAATAGTGAGTGCACTTTATGGCCGCGAAAAGAGCCAGAGAGGGGCGCATGTCGATATAGCGATGTTTGATGCCACGCTGAGTTTTCTGGAGCATGGTCTGATGGCATATATCGCAACTGGGAAGTCACCACAACGTCTGGGAAATCGCCATCCCTACATGGCACCTTTTGATGTTTTCAATACTCAGGATAAGCCGATTACGATTTGTTGTGGTAATGACAAGCTTTTTTCTGCGTTATGCCAGGCACTGGAGCTTACGGAACTGGTTAATGATCCCCGATTTAGCAGCAATATTTTACGCGTACAAAACCAGGCTATTCTTAAACAATATATTGAGCGGACGTTAAAAACGCAGGCAGCTGAAGTTTGGTTAGCCAGAATACATGAAGTTGGTGTACCCGTCGCGCCGTTATTAAGTGTGGCTGAGGCCATTAAATTGCCACAAACTCAGGCGAGAAATATGTTGATTGAAGCCGGGGGAATAATGATGCCGGGTAATCCGATAAAAATCAGCGGCTGCGCGGACCCGCATGTTATGCCGGGAGCGGCAACGCTCGACCAGCATGGGGAACAAATTCGCCAGGAGTTCTCATCATAAGGTTCCTAAAAACCCTGGCATCTTTTCCATAACAAAAATTAAACCTCCTCCATATGAAAAATAAAAATTATAAAATGACAAGTGCCGCTACTGTTACTGCTTCGTTCAACGATACTTTTAGCGTATCCGATAATGTCGCCGTTATTGTTCCTGAAACGGACACTCAGGTGACCTACAGGGATCTATCCCACATGGTGGGTCACTTCCAGACCATGTTCACAAATCCTAATTCTCCATTGTACGGAGCTGTTTTCAGACAAGATACAGTGGCGATATCCATGCGTAATGGGCTGGAATTTATCGTCGCTTTCCTCGGTGCTACTATGGACGCTAAAATTGGCGCGCCCTTGAATCCCAATTATAAGGAAAAGGAGTTCAATTTTTATTTGAATGACCTGAAATCTAAGGCGATTTGCGTCCCAAAGGGTACCACAAAGTTACAGAGTTCTGAAATTCTAAAATCTGCCTCCACGTTTGGATGTTTTATCGTAGAGCTGGCCTTCGATGCGACCAGGTTTAGGGTAGAGTATGATATATACTCTCCAGAGGACAACTACAAAAGGGTTATTTACCGGTCTTTGAACAACGCCAAATTTGTCAACACAAATCCCGTTAAATTCCCTGGGTTTGCCCGTTCCAGTGACGTTGCCCTGATTTTGCATACCAGTGGTACCACCTCCACTCCAAAAACGGTGCCTTTGTTACATTTGAACATTGTGAGAAGCACGTTGAACATTGCTAACACTTACAAGCTAACGCCCTTGGACAGATCTTATGTCGTGATGCCTCTTTTCCACGTCCATGGGTTAATTGGTGTTTTACTTTCCACTTTTAGAACTCAGGGTTCTGTTGTGGTTCCCGATGGATTCCATCCAAAGTTATTCTGGGACCAATTTGTTAAGTACAACTGTAATTGGTTCAGTTGCGTTCCCACAATAAGCATGATTATGCTGAACATGCCCAAACCAAACCCTTTCCCACACATTAGATTCATCAGATCGTGTTCTTCTGCTTTGGCTCCAGCAACGTTCCATAAGCTGGAGAAGGAATTCAATGCACCTGTCTTGGAGGCCTATGCGATGACCGAAGCATCACATCAAATGACCTCAAACAATCTGCCTCCAGGAAAGAGAAAGCCTGGTACTGTGGGCCAGCCACAAGGAGTCACCGTCGTCATTCTAGATGACAATGACAATGTCTTGCCCCCGGGCAAAGTCGGCGAAGTTTCCATCAGAGGCGAAAACGTCACTTTGGGGTATGCTAATAATCCAAAAGCTAACAAGGAGAACTTCACCAAGAGAGAGAACTATTTCAGAACCGGTGACCAAGGTTATTTCGACCCTGAGGGGTTTTTGGTCCTTACAGGCAGAATCAAAGAGCTTATCAACAGGGGTGGTGAAAAGATTTCACCCATTGAGCTCGACGGCATTATGCTATCGCATCCAAAGATCGATGAAGCCGTTGCATTTGGTGTTCCCGACGATATGTACGGCCAAGTAGTTCAAGCCGCCATTGTTTTGAAGAAGGGAGAAAAAATGACCTACGAAGAACTGGTGAACTTCTTAAAGAAGCACCTAGCCTCTTTCAAAATTCCAACCAAGGTGTACTTTGTTGATAAGCTACCAAAAACCGCTACAGGTAAAATCCAGAGAAGAGTTATCGCAGAAACTTTTGCTAAGAGCAGCAGAAATAAGAGTAAGTTGTAGGGTTCCTAGCTGATTAGAAGGCCATCCTGACGGATGGCCTTTTTTTTGACTCTGTTGTGTCTTCTTTTAGGTGTGGAATAATTTTCCAACCATCACTTTATCTGTCTCCCGAACAGCACCTTATTCAGCCATCGGTTCACATACACGTTCACGAAAGCACATCCTACTCCGATAGCGGCTCCTGCCACTACATCACTGGGATAGTGCACCCCTTGGTTCATCCGGGCAAACCCTACTCCGCAAGCCCATACGGCTGAAGGGGCGATAACATACCACTTAGGATAAGTGATGGAGAGTGAGGTGGCTAAAGAAAAAGCTGCCGCAGTGTGTCCTGAAGGGAATGACGGACTATCGGCATCCGGTGCTCCGTATGCATGAATCTTATCCGGATACTTTACAAAAGGCCGGTCACGACCTATTATATGTTTGGCGGCGTATGTAATCCCCACTGCTTCAATCACACTGGTTCCTATGTAAATGGCATCTTTCAGCATCGGCTGGTCTTTCTTTATCAATGCATAGAGTCCCATGGCTGTAGGTATTCCTACGGGTAGAATCACTCCGCTAGCGGACAATCCGCGGCTCAGTCCGTGTACATCCCATCGGTTGATGGTTTTCAACGTGTTGATGTCTATATTCTGTGCGCTGAGGCTGAATGTAAAACAGGCTGTCAGTATAAGAAAAATAATCCTTTGGCGTTTCATTTAGTTTGGCATTTGTTCAGTGCAAAGATAGGAAAATAATGTAATTCGCATTTACCCTTCCCCGAATTAAAGAATAATTTCCATAATTCCTTGTATTATCGCTAGAATTCCTTTACTTTTGCATCGTGAATACACAGCTTTTAACAGAATTTTTAGTTCTAACAATTAAATAATTTAAATTCAATCATTTATGTGGTTAAGTAATTCATCTATTGGAAGGAAAGTGGTGATGAGTGTCACAGGTATCGCCCTTGTCCTGTTTCTGACGTTTCACATGGCGATGAACCTTGTTGCGCTCTTCTCGGAAGATGCTTACAACATGATTTGTGAATTCTTGGGAGCTAACTGGTACGCGTTGGTTGCAACTGCCGGGCTGGCTGCTCTGTTTGTAATTCACATCATCTACGCTTTCTGGTTGACCATGCAGAACCGTGCAGCTCGCGGTAGCGAACGTTATGCAGTGAACGTTAAACCTAAAACTGTGGAATGGGCATCTCAGAACATGCTTGTCCTGGGTATTGTCGTTATTGCCGGATTGGCTCTTCATTTTGCCAATTTCTGGTACAAAATGCAGTTTGCCGAAATTATCGGTGTTCATGATTTGGGCGCATTCGGTCCTACAGACGGTGCCGCTTACATTCGTGAAACATTCGGATGCCCTGTATTTACAGTGCTTTACCTCATTTGGTTAGCAGCTTTGTGGTTCCACCTGACTCACGGATTCTGGAGTGCTTTGCAGACTTTGGGCTGGAGCAATAAAATCTGGTTCGAGCGTTGGAAGACTATTGGTAACATCTACTCTACTTTGGTAGTTCTTGGTTTTGCAGCAGTAGTCGTTATCTACTTCGCTATGACTGGCGCGCCTGATAGGTGGGCTGCCCTTCCTGGTTGGCTTGGTTTCATCAGCCATCCGCTTGCCCTCATCTGTTACGCCGGCGGTAGCCGGCCAGCCTCGCAGAGCAGGATTCCCGTTGAGCACCGCCAGGTGCGAATAAGGGACAGTGAAGAAGGAACACCCGCTCGCGGGTGGGCCTACTTCACCTATCCTGCCCGGCTGACGCCGTTGGATACACCAAGGAAAGTCTACACGAACCCTTTGGCAAAATCCTGTATATCGTGCGAAAAAGGATGGATATACCGAAAAAATCGCTATAATGACCCCGAAGCAGGGTTATGCAGCGGAAAAGCGGGATTAAAAGTCGGGGATTGGTGAACAAAAAGGTGTTTCTCTCTTTAAGAGAAATATCGTTTTGCTAAACAGTTGATATTGAGGTATCATTTTATCGTAAAAGACATTTTTGCTCAACAATTGCTTGACGGAAATCAACAAATTTTAGCATTTTGTAAAAAAGTCGCTATATAATTTGGTGAATTGGAGTTATTTTCATATTTTTGCATCCCGAAGAGTTTCTCTTAAAGAGAGAAACATCTTTTGCATACCTTTTCCGACCGAATTTTTATGTCGTAAAGAGGGGCTTTGCAGGGGGTGGACTCAGAAAGATGAGAATAGATGACTATTGTAGTTGAAACACATAGAAAGTTGCTGATATACAGACCGATACGCATATCGGGATGAACCATGAGTACGTTCTTTTCTCAAAAAACATAAATATTCGAAAAGAGATGCAATAAATTAAGGAGAGGTTATAATGAACAAAGTAAATATAAAAGATAGTCAAAATTTTATTACTTCAAAATATCACATAGAAAAAATAATGAATTGCATAAGTTTAGATGAAAAAGATAACATCTTTGAAATAGGTGCAGGGAAAGGTCATTTTACTGCTGGATTGGTAAAGAGATGTAATTTTGTAACGGCGATAGAAATTGATTCTAAATTATGTGAGGTAACTCGTAATAAGCTCTTAAATTATCCTAACTATCAAATAGTAAATGATGATATACTGAAATTTACATTTCCTAGCCACAATCCATATAAAATATTTGGCAGCATACCTTACAACATAAGCACAAATATAATTCGAAAAATTGTTTTTGAAAGTTCAGCCACAATAAGTTATTTAATAGTGGAATATGGTTTTGCTAAAATGTTATTAGATACAAACAGATCACTAGCATTGCTGTTAATGGCAGAGGTAGATATTTCTATATTAGCAAAAATTCCTAGGTATTATTTCCATCCAAAACCTAAAGTGGATAGCACATTAATTGTATTAAAAAGAAAGCCAGCAAAAATGGCATTTAAAGAGAGAAAAAAATATGAAACTTTTGTAATGAAATGGGTTAACAAAGAGTACGAAAAACTGTTTACAAAAAATCAATTTAATAAAGCTTTAAAACATGCGAGAATATATGATATAAACAATATTAGTTTCGAACAATTTGTATCGCTATTTAATAGTTATAAAATATTTAACGGCTAAAAACAATAGGCCACATGCAACTGTAAATGTTTACGCGGGTACCGACACCGCGGTGGAGGGGAATTGTGTTACAACCAATTAACCAATTCTGATTAGAAAAACTCATCGAGCATCAAATGAAACTGCAATTTATTCATATCAGGATTATCAATACCATATTTTTGAAAAAGCCGTTTCTGTAATGAAGGAGAAAACTCACCGAGGCAGTTCCATAGGATGGCAAGATCCTGGTATCGGTCTGCGATTCCGACTCGTCCAACATCAATACAACCTATTAATTTCCCCTCGTCAAAAATAAGGTTATCAAGTGAGAAATCACCATGAGTGACGACTGAATCCGGTGAGAATGGCAAAAGCTTATGCATTTCTTTCCAGACTTGTTCAACAGGCCAGCCATTACGCTCGTCATCAAAATCACTCGCATCAACCAAACCGTTATTCATTCGTGATTGCGCCTGAGCGAGGCGAAATACGCGATCGCTGTTAAAAGGACAATTACAAACAGGAATCGAATGCAACCGGCGCAGGAACACTGCCAGCGCATCAACAATATTTTCACCTGAATCAGGATATTCTTCTAATACCTGGAATGCTGTTTTCCCGGGGATCGCAGTGGTGAGTAACCATGCATCATCAGGAGTACGGATAAAATGCTTGATGGTCGGAAGAGGCATAAATTCCGTCAGCCAGTTTAGTCTGACCATCTCATCTGTAACATCATTGGCAACGCTACCTTTGCCATGTTTCAGAAACAACTCTGGCGCATCGGGCTTCCCATACAATCGATAGATTGTCGCACCTGATTGCCCGACATTATCGCGAGCCCATTTATACCCATATAAATCAGCATCCATGTTGGAATTTAATCGCGGCCTGGAGCAAGACGTTTCCCGTTGAATATGGCTCATAACACCCCTTGTATTACTGTTTATGTAAGCAGACAGTTTTATTGTTCATGATGATATATTTTTATCTTGTGCAATGTAACATCAGAGATTTTGAGACACAACGTGGCTTTGTTGAATAAATCGAACTTTTGCTGAGTTGAAGGATCAGAGCCTACGTTCCGAATACGGTCAAAAAAAAGGCCATCCGTCAGGATGGCCTTCGCATTAATATGCCGCTTCGAATTCTTTTAGGAAGCGTGTATCGTTTTCAGAGAACATACGGAGGTCTTTCACCTGATATTTCAGGTTTGTGATACGCTCGATACCCATACCGAGTCCATAACCGCTGTATATTTTGCTGTCTATACCATTTGATTCAAGTACGTTCGGGTCTACCATACCGCAACCGAGGATTTCTACCCAGCCGGTGTGTTTACAGAACGGACATCCTTTACCGCCGCAGATATTACAGCTGATATCCATTTCCGCACTTGGTTCAGCAAACGGGAAGTAAGACGGACGCAGACGGATCTTTGTATCAGCACCGAACATTTCTTTGGCAAAGAGCAGCAATACCTGCTTCAAGTCGGTGAATGATACGTTTTTATCTACATACAGCGCTTCTACCTGATGGAAGAAACAGTGTGCGCGATAGCTGATAGCTTCGTTACGATATACACGTCCCGGACAGATGATGCGGATAGGAGGCTGTGAAGTTTCCATCACACGAGTCTGTACAGAAGAAGTATGTGTACGCAATACTACGTCCGGGTGAGCTTCGATAAAGAAAGTGTCCTGCATATCGCGTGCCGGATGATCTTCGGCAAAGTTCAGTGCCGAGAACACGTGCCAGTCATCTTCAATTTCCGGACCTTCGGCAATGCTGAATCCCAGACGGGCAAAGATATCAATGATTTCGTTCTTTACAATGGTGAGCGGGTGGCGTGTACCGAGTTCTACAGGATAAGCCGAACGCGTCAAATCCAGTCCGTCACAATCGTTGTCCTGACTTTCAAACATTTCTTTCAGCGCGTTGATTTTGTCCTGCGCTTTTGTTTTCAGTTCATTCAGTCTCATGCCGACTTCTTTTTTCTGTTCGGCAGCTACATTACGGAAATCTGCCATTAAGTCGTTAATGGCTCCCTTCTTACTTAGGTATTTGATGCGGAGAGCTTCGAGTTCTTCGGCATTGGAGGCGTGTAAGGCTTCCACCTCTTTCAGAAGTTGTTCAATCTTAGCTATCATTTTTTAATATTTTTAGCGGCCCCGTTAAACAAAATTATTTGTAGAGGCTGTTTCGTCCTCACGGACTCATCAGACCGGAAAGCACATCCGGTGACAGCTCAGGCTACTTTGTTTCTTTCGACACTGCAAATATAAGAACATTATTTGAAAGTTCAAGTGAAACTTTAAATTTTAACAATAGATTAACCATTGCAAACAAAACAAAAAAAAGGTAGCCCAATTGTAAAACGAAAGGCCCAGTCTTTCGACTGAGCCTTTCGTTTTATCCTACAGTCGCTCGGCGATCGAAGGCTTCGGAAAAAAAAGGCCATCCGTCAGGATGGCCTTCGCATTAATATGCCGCTTCGAATTCTTTTAGGAAGCGTGTATCGTTTTCAGAGAACATACGGAGGTCTTTCACCTGATATTTCAGGTTTGTGATACGCTCGATACCCATACCGAGTCCATAACCGCTGTATATTTTGCTGTCTATACCATTTGATTCAAGTACGTTCGGGTCTACCATACCGCAACCGAGGATTTCTACCCAGCCGGTGTGTTTACAGAACGGACATCCTTTACCGCCGCAGATATTACAGCTGATATCCATTTCCGCACTTGGTTCAGCAAACGGGAAGTAAGACGGACGCAGACGGATCTTTGTATCAGCACCGAACATTTCTTTGGCAAAGAGCAGCAATACCTGCTTCAAGTCGGTGAATGATACGTTTTTATCTACATACAGCGCTTCTACCTGATGGAAGAAACAGTGTGCGCGATAGCTGATAGCTTCGTTACGATATACACGTCCCGGACAGATGATGCGGATAGGAGGCTGTGAAGTTTCCATCACACGAGTCTGTACAGAAGAAGTATGTGTACGCAATACTACGTCCGGGTGAGCTTCGATAAAGAAAGTGTCCTGCATATCGCGTGCCGGATGATCTTCGGCAAAGTTCAGTGCCGAGAACACGTGCCAGTCATCTTCAATTTCCGGACCTTCGGCAATGCTGAATCCCAGACGGGCAAAGATATCAATGATTTCGTTCTTTACAATGGTGAGCGGGTGGCGTGTACCGAGTTCTACAGGATAAGCCGAACGCGTCAAATCCAGTCCGTCACAATCGTTGTCCTGACTTTCAAACATTTCTTTCAGCGCGTTGATTTTGTCCTGCGCTTTTGTTTTCAGTTCATTCAGTCTCATGCCGACTTCTTTTTTCTGTTCGGCAGCTACATTACGGAAATCTGCCATTAAGTCGTTAATGGCTCCCTTCTTACTTAGGTATTTGATGCGGAGAGCTTCGAGTTCTTCGGCATTGGAGGCGTGTAAGGCTTCCACCTCTTTCAGAAGTTGTTCAATCTTAGCTATCATTTTTTAATATTTTTAGCGGCCCCGTTAAACAAAATTATTTGTAGAGGCTGTTTCGTCCTCACGGACTCATCAGACCGGAAAGCACATCCGGTGACAGCTCAGGCTACTTTGTTTCTTTCGACACTGCAAATATAAGAACATTATTTGAAAGTTCAAGTGAAACTTTAAATTTTAACAATAGATTAACCATTGCAAACAAAACAAAAAAAAGGTAGCCCAATTGTAAAACGAAAGGCCCAGTCTTTCGACTGAGCCTTTCGTTTTATCCTAGGATCAGCTGTACGTACTCGCAGTTCAACCTGTTGATAGTACGTACTAAGCTCTCATGTTTCACGTACTAAGCTCTCATGTTTAACGTACTAAGCTCTCATGTTTAACGAACTAAACCCTCATGGCTAACGTACTAAGCTCTCATGGCTAACGTACTAAGCTCTCATGTTTCACGTACTAAGCTCTCATGTTTGAACAATAAAATTAATATAAATCAGCAACTTAAATAGCCTCTAAGGTTTTAAGTTTTATAAGAAAAAAAAGAATATATAAGGCTTTTAAAGCTTTTAAGGTTTAACGGTTGTGGACAACAAGCCAGGGATGTAACGCACTGAGAAGCCCTTAGAGCCTCTCAAAGCAATTTTGAGTGACACAGGAACACTTAACGGCTGACATGGGGCGGCCGCGTAG
SEQ ID NO:92(用于基因组整合进普通拟杆菌基因组中的编码草酸盐降解途径的质粒):
GGAGAGCTGTATGTGGGGTGGAAGAAGCTATCGTAAAAATGCTGGAGCAGCTTGATCCTCATTCTACCTATTCTGATCCGGAGGAAGTGAAGAGAATGAACGAGCCGTTGCAGGGAAACTTCGAAGGTATCGGTATACAGTTTAATATGGCGGAGGACACTTTGCTGGTGATACAGCCTGTATCGGGCGGACCGTCCGAAAAAGTCGGTATTCTGGCCGGTGATCGTATCGTCATGGTGGAAGATACGCTGATAGCCGGTGTAAAGATGAGTACCGAGGATATTATGCGTCGCCTCCGTGGTCCTAAAGATTCAAAAGTGAATCTGAAAATACTTCGCCGAGGGGTGAAGGAACTGCTCCCCTTTACGGTGAAAAGGGATAAAATACCTGTTTACAGCTTGGATGCTTCCTATATGATTAAAGATAAGATCGGTTATATTCGTATCAACCGTTTTGCCGCCACTACCCATGAGGAGTTCAAGAAAGCGCTGACCGGGTTGCAGAAGAAAGGAATGAAAGACTTGATTCTTGATTTGCAGGGCAATGGCGGGGGGTATTTGAATGCTGCTATCGATATTGCCAATGAATTTTTGGGTAAAAAGGAACTGATTGTCTATACGGAGGGCCGTCGTAATCCTCGTAGCGAGTTTTTTGCCAAAGGTACGGGCGAGTTTCAGAACGGTCGTCTGATGGTATTGGTTGATGAATTTTCGGCTTCTGCCAGTGAGATTGTGACCGGAGCTGTTCAGGATTGGGACAGAGGCGTTGTGGTAGGCCGTCGCAGTTTTGGTAAAGGCTTGGTGCAGCGTCCCATTGATTTGCCGGACGGTTCGATGATCCGTCTGACGGTGGCCCGCTATTATACGCCTGCCGGACGTTGTATCCAGAAACCTTACGAGAGCATTGAAAAGTATAATGAAGACCTGATAGACCGTTATAATAAAGGAGAAATGATGAGTGAAGACAGCATCCACTTTCCGGATTCACTGAAGTTCAAGACTCATAGGTTGGCCCGTACGGTATATGGTGGCGGAGGGATCATGCCCGATTATTTTGTGCCGATTGATACGACTCTCTATACTAAATACCATCGTCAGTTGCGTGATAAAGGAGCATTGATGAAAGCTCATTTCCATTTTATTGATGCCCATCGTAAAGAATGGCTGGGCAAATACAAGACTTTCAATGAGTTCTACAAACGTTTTGAAGTGACTCCGGATATGTTGACACAACTGGTTGCTACCGGCAAGGAGATGGGAGTGGAATATAATGAAGAAGAGTATCAAAAGGCCCTGCCTCTGCTGAGGTTGCAGATGAAAGCATTGATAGCCCGTGATTTATGGGATATGAATGAATATTATCACGTCATTAATGACGCGAATGAAAGTATCAGGAAAGCGCTGGAACTGTTGGAGCAACCGGACTTTGAGGGGCTGCTGTTGAAAAAGCGATAGTCAAGACTTTATATTAACTGCAGGGGCGCATAGTTGCACTCCTGCAGTTGTCTGAAAAACTTGTAGGATAAAACGAAAGGCTCAGTCGAAAGACTGGGCCTTTCGTTTTACAATTGGGCTACCTTTTTTTTGTTTTGTTTGCAATGGTTAATCTATTGTTAAAATTTAAAGTTTCACTTGAACTTTCAAATAATGTTCTTATATTTGCAGTGTCGAAAGAAACAAAGTAGCCTGGCATCATCTACCTTCATTATTAAACAAACCAAATTAATATCATAAAAAAATAAAAAAATGACTACCACAGCAACGCAGCATTTTGCCCAGTTACTCCAGAAGTACGGAATTGATACTGTCTTCGGCATAGTAGGTATTCCGATCGTGCAGTTGGCCGACACTATGGTCGCGAATGGAATTAAGTTTATACCGTGTCGCAATGAGCAAGCCGCAAGTTACGCGGCAAGCGCCTACGGCTACATCTCGGACAAACCTGGTGTCCTGTTAATCGTGGGAGGGCCTGGCCTTATTCATGCCTTAGCGGGGATCTATAATAGTATGTCGAACCGCTGGCCCTTGTTGGTCATCGCCGGATCCAGCAGCCAGTCAGACATCCACAAGGGAGGTTTCCAGGAACTTGACCAGGTGTCCTTGCTAAGTCCGTTCCTAAAATTCACTGGTAAACTGACGCCGGATAACATCGATATGATAACCCAAAAAGCACTCAACTATTGCATTCAGGGAACCGCCGGAGTGAGCTATATCGATGTCCCCGCTGACTTCATCGAATACGAGAAGCCGCTTGAGGGCAATGATAGAACTGGTAACGAGCTGCCAATGATCCTGACGCCCAATATCTGCGGTCCAGACCCCAGTAAAATTAAGAAGGTGGTGCAATTGATACTCCAGCACAAGAACAAGAACATATTAATAGTGATAGGTAAAGGAGCAGTGAAGAATAGCCATGAGATTAGACGCTTGGTTAACACCTTTAACTTGCCGTTCCTTCCGACTCCCATGGCGAAAGGCATAGTGCCCGATAGCAGCCCTTTAAATGTGAGCAGCGCCCGGAGCCAGGCTCTTAAGATTGCTGACATCGTGTTAGTATTGGGAGCTCGCCTGAATTGGATCCTTCACTTCGGAACGAGTCCAAAGTGGAATAGCGAGAGTATTTTTATACAATTTGACAGTAACCCGGAAACGCTCGGAGACAATAACGTTAGTCCCGGAGCAGATTTAAGTATCTGGGGGGACATCGGACTATCGGTTACTGCACTGGTAGAAGAGCTCACGAGACAGGACAGTTGTTGGAAATACTCGGGAGTCAAACAGGAGATCAGGGAAAAGATACAGTTAAACCAGACCAGATTGCTCCGTAAGGAAAAAACGCGTGGAGCTCAGCTTAACTATAATCAAGTGTACGGCACCCTTCGTCCGTTAATTGACGACTATCGCACGATCTTGGTCACAGAAGGTGCCAACACAATGGACATCGCTAGAATAAGTTTTCCGACTGATGCACCTCGCAGAAGACTTGATGCTGGTACAAATGCTACAATGGGTATAGGTTTAGGTTACGCATTAGCGTGTAAAGCCAGCCACCCTGAGTTAGACGTTGTTTTAATCCAAGGCGACAGTGCCTTCGGTTTTAGCGCTATGGAGATCGAGACTGCCGTCCGTTGCCAATTAGCCCTTGTGATTGTCGTAATGAATAACTCTGGGATATATCACGGTGAGAAAGACATCGAAGGAGATCTGCCCCCGACCGCTTTGTCAAAAAATTGTAGATATGACTTGGTCGGTAAGGGTTTAGGAGCTAACGACTTCTTCGTCAATACCATCTCAGAGTTATCTCGCTGTTTTCAGCAGGCAGTTCAATTGAGCAGAACCAAGCGCGAAACCTCCGTCATTAATGTCATAATCGAACCGGGAGAGCAGAAACAGATCGCGTTCGCATGGCAAAACAAGCCTAGACTGTAAGGTTCCTAACCTGCCTGGCATCTTAATCAATTTTAAACTCACCAAATCAAAATATGAAAAAAAAAAATAATAAAATGTCAACTCCACTTCAAGGAATTAAAGTTCTCGATTTCACCGGTGTGCAATCTGGCCCATCTTGTACTCAAATGCTGGCCTGGTTTGGCGCTGACGTTATTAAAATTGAACGTCCCGGCGTTGGTGACGTAACGCGTCACCAGCTGCGAGATATTCCTGATATCGATGCGCTTTACTTCACCATGCTTAACAGTAACAAACGTTCTATTGAGTTAAATACCAAAACAGCGGAAGGCAAAGAGGTAATGGAAAAGCTGATCCGCGAAGCTGATATCTTAGTCGAGAACTTTCATCCAGGGGCCATTGATCACATGGGCTTCACCTGGGAGCATATTCAAGAAATCAATCCACGTCTGATTTTTGGTTCGATCAAAGGGTTTGATGAGTGTTCGCCTTATGTGAATGTAAAAGCCTATGAAAACGTTGCTCAGGCAGCGGGTGGCGCGGCATCCACTACGGGTTTTTGGGATGGTCCGCCGCTGGTAAGCGCTGCAGCGTTGGGTGACAGCAACACCGGAATGCATTTGCTGATCGGTTTACTTGCTGCTTTGCTGCATCGCGAAAAAACGGGGCGTGGGCAACGAGTCACCATGTCAATGCAGGATGCCGTATTGAACCTTTGCCGCGTGAAATTACGTGACCAGCAGCGTCTAGATAAATTGGGTTATCTGGAAGAATACCCGCAGTATCCGAATGGTACATTTGGTGATGCAGTTCCCCGCGGTGGTAATGCAGGTGGTGGCGGTCAGCCTGGCTGGATCCTGAAATGTAAAGGCTGGGAAACCGATCCTAACGCCTATATTTATTTCACTATTCAGGAGCAAAACTGGGAAAACACCTGTAAAGCCATCGGCAAACCAGAATGGATTACCGATCCGGCATACAGTACAGCCCATGCACGACAGCCACATATTTTCGATATTTTTGCTGAAATCGAAAAATACACTGTCACTATTGATAAACATGAAGCGGTGGCCTATTTGACTCAGTTTGATATTCCTTGTGCACCGGTTTTAAGTATGAAAGAAATTTCACTTGATCCCTCTTTGCGCCAAAGTGGCAGTGTTGTTGAAGTGGAACAACCGTTGCGTGGAAAATATCTGACCGTTGGTTGTCCAATGAAATTCTCTGCCTTTACGCCGGATATTAAAGCTGCGCCGCTATTAGGTGAACATACCGCTGCTGTATTGCAGGAGCTGGGTTATAGCGACGATGAAATTGCTGCAATGAAGCAAAACCACGCCATCTGAGGTTCCTAAACGACCTGGCATCATCTACCTTTATTAATCAACAATCCAAATTATTAATAAAAAAAATTTAAAAAATGACAAATAATGAAAGCAAAGGGCCGTTTGAAGGCTTATTAGTTATCGATATGACACATGTCCTTAATGGACCTTTCGGAACTCAACTTCTTTGTAATATGGGCGCAAGGGTAATTAAAGTTGAGCCGCCGGGTCATGGTGATGATACCCGCACATTTGGTCCCTATGTGGATGGACAGTCACTCTATTACAGTTTTATTAATCATGGCAAAGAGAGTGTGGTTCTTGATTTAAAGAATGATCACGATAAAAGTATATTTATAAATATGCTCAAACAAGCTGATGTATTAGCTGAGAATTTTCGCCCAGGTACAATGGAAAAACTGGGGTTTTCATGGGAAACGCTTCAAGAAATCAACCCGCGCCTCATATATGCTTCATCGTCAGGTTTCGGACATACCGGTCCGCTAAAAGATGCTCCTGCCTACGATACCATCATTCAGGCAATGAGCGGGATAATGATGGAAACAGGATATCCTGATGCTCCGCCAGTGCGCGTTGGTACATCTCTTGCGGATCTATGCGGCGGTGTCTATTTATTCAGCGGAATAGTGAGTGCACTTTATGGCCGCGAAAAGAGCCAGAGAGGGGCGCATGTCGATATAGCGATGTTTGATGCCACGCTGAGTTTTCTGGAGCATGGTCTGATGGCATATATCGCAACTGGGAAGTCACCACAACGTCTGGGAAATCGCCATCCCTACATGGCACCTTTTGATGTTTTCAATACTCAGGATAAGCCGATTACGATTTGTTGTGGTAATGACAAGCTTTTTTCTGCGTTATGCCAGGCACTGGAGCTTACGGAACTGGTTAATGATCCCCGATTTAGCAGCAATATTTTACGCGTACAAAACCAGGCTATTCTTAAACAATATATTGAGCGGACGTTAAAAACGCAGGCAGCTGAAGTTTGGTTAGCCAGAATACATGAAGTTGGTGTACCCGTCGCGCCGTTATTAAGTGTGGCTGAGGCCATTAAATTGCCACAAACTCAGGCGAGAAATATGTTGATTGAAGCCGGGGGAATAATGATGCCGGGTAATCCGATAAAAATCAGCGGCTGCGCGGACCCGCATGTTATGCCGGGAGCGGCAACGCTCGACCAGCATGGGGAACAAATTCGCCAGGAGTTCTCATCATAAGGTTCCTAAGGGTCCTGGCATCACCTAAAAATTATAAATAAATTAATAAACCTTTTAACATAAAAATTAATAAAATGAATAATCCACAAACAGGACAATCAACAGGCCTCTTGGGCAATCGTTGGTTCTACTTGGTATTAGCAGTTTTGCTGATGTGTATGATCTCGGGTGTCCAATATTCCTGGACACTGTACGCTAACCCGGTTAAAGACAACCTTGGCGTTTCTTTGGCTGCGGTTCAGACGGCTTTCACACTCTCTCAGGTCATTCAAGCTGGTTCTCAGCCTGGTGGTGGTTACTTCGTTGATAAATTCGGTCCAAGAATTCCATTGATGTTCGGTGGTGCGATGGTTCTCGCTGGCTGGACCTTCATGGGTATGGTTGACAGTGTTCCTGCTCTGTATGCTCTTTATACTCTGGCCGGTGCAGGTGTTGGTATCGTTTACGGTATCGCGATGAACACGGCTAACAGATGGTTCCCGGACAAACGCGGTCTGGCTTCCGGTTTCACCGCTGCCGGTTACGGTCTGGGTGTTCTGCCGTTCCTGCCACTGATCAGCTCCGTTCTGAAAGTTGAAGGTGTTGGCGCAGCATTCATGTACACCGGTTTGATCATGGGTATCCTGATTATCCTGATCGCTTTCGTTATCCGTTTCCCTGGCCAGCAAGGCGCCAAAAAACAAATCGTTGTTACCGACAAGGATTTCAATTCTGGCGAAATGCTGAGAACACCACAATTCTGGGTTCTGTGGACCGCATTCTTTTCCGTTAACTTTGGTGGTTTGCTGCTGGTTGCCAACAGCGTCCCTTACGGTCGCAGCCTCGGTCTTGCCGCAGGTGTGCTGACGATCGGTGTTTCGATCCAGAACCTGTTCAATGGTGGTTGCCGTCCTTTCTGGGGTTTCGTTTCCGATAAAATCGGCCGTTACAAAACCATGTCCGTCGTTTTCGGTATCAATGCTGTTGTTCTCGCACTTTTCCCGACGATTGCTGCCTTGGGCGATGTAGCCTTTATCGCCATGTTGGCAATCGCATTCTTCACATGGGGTGGTAGCTACGCTCTGTTCCCATCGACCAACAGCGATATTTTCGGTACGGCATACTCTGCCAGAAACTATGGTTTCTTCTGGGCTGCAAAAGCAACTGCCTCGATCTTCGGTGGTGGTCTGGGTGCTGCAATTGCAACCAACTTCGGATGGAATACCGCTTTCCTGATTACTGCGATTACTTCTTTCATCGCATTTGCTCTGGCTACCTTCGTTATTCCAAGAATGGGCCGTCCAGTCAAGAAAATGGTCAAATTGTCTCCAGAAGAAAAAGCTGTACATTAAGGTTCCTAATTCCCCTGGCATCCCCAAAAAATTAATTCATTTTCTCTTTTCAACAATTTAAAAAAAATTAAAAAATGAGTAACGACGACAATGTAGAGTTGACTGATGGCTTTCATGTTTTGATCGATGCCCTGAAAATGAATGACATCGATACCATGTATGGTGTTGTCGGCATTCCTATCACGAACCTGGCTCGTATGTGGCAAGATGACGGTCAGCGTTTTTACAGCTTCCGTCACGAACAACACGCAGGTTATGCAGCTTCTATCGCCGGTTACATCGAAGGAAAACCTGGCGTTTGCTTGACCGTTTCCGCCCCTGGCTTCCTGAACGGCGTGACTTCCCTGGCTCATGCAACCACCAACTGCTTCCCAATGATCCTGTTGAGCGGTTCCAGTGAACGTGAAATCGTCGATTTGCAACAGGGCGATTACGAAGAAATGGATCAGATGAATGTTGCACGTCCACACTGCAAAGCTTCTTTCCGTATCAACAGCATCAAAGACATTCCAATCGGTATCGCTCGTGCAGTTCGCACCGCTGTATCCGGACGTCCAGGTGGTGTTTACGTTGACTTGCCAGCAAAACTGTTCGGTCAGACCATTTCTGTAGAAGAAGCTAACAAACTGCTCTTCAAACCAATCGATCCAGCTCCGGCACAGATTCCTGCTGAAGATGCTATCGCTCGCGCTGCTGACCTGATCAAGAACGCCAAACGTCCAGTTATCATGCTGGGTAAAGGCGCTGCATACGCACAATGCGACGACGAAATCCGCGCACTGGTTGAAGAAACCGGCATCCCATTCCTGCCAATGGGTATGGCTAAAGGCCTGCTGCCTGACAACCATCCACAATCCGCTGCTGCAACCCGTGCTTTCGCACTGGCACAGTGTGACGTTTGCGTACTGATCGGCGCTCGTCTGAACTGGCTGATGCAGCACGGTAAAGGCAAAACCTGGGGCGACGAACTGAAGAAATACGTTCAGATCGACATCCAGGCTAACGAAATGGACAGCAACCAGCCTATCGCTGCACCAGTTGTTGGTGACATCAAGTCCGCCGTTTCCCTGCTCCGCAAAGCACTGAAAGGCGCTCCAAAAGCTGACGCTGAATGGACCGGCGCTCTGAAAGCCAAAGTTGACGGCAACAAAGCCAAACTGGCTGGCAAGATGACTGCCGAAACCCCATCCGGAATGATGAACTACTCCAATTCCCTGGGCGTTGTTCGTGACTTCATGCTGGCAAATCCGGATATTTCCCTGGTTAACGAAGGCGCTAATGCACTCGACAACACTCGTATGATTGTTGACATGCTGAAACCACGCAAACGTCTTGACTCCGGTACCTGGGGTGTTATGGGTATTGGTATGGGCTACTGCGTTGCTGCAGCTGCTGTTACCGGCAAACCGGTTATCGCTGTTGAAGGCGATAGCGCATTCGGTTTCTCCGGTATGGAACTGGAAACCATCTGCCGTTACAACCTGCCAGTTACCGTTATCATCATGAACAATGGTGGTATCTATAAAGGTAACGAAGCAGATCCACAACCAGGCGTTATCTCCTGTACCCGTCTGACCCGTGGTCGTTACGACATGATGATGGAAGCATTTGGCGGTAAAGGTTATGTTGCCAATACTCCAGCAGAACTGAAAGCTGCTCTGGAAGAAGCTGTTGCTTCCGGCAAACCATGCCTGATCAACGCGATGATCGATCCAGACGCTGGTGTCGAATCTGGCCGTATCAAGAGCCTGAACGTTGTAAGTAAAGTTGGCAAGAAATAAGGTTCCTAAAATGCCTGGCATCTAAACTAAAATACAATAACCAAAAATCATCTATGCGTAAAAAAAATTATAAAATGGATAGCGATACTCTCTCAGGATTATTGGAAAACGTCGCCAAAAAATTCCCCGATCGCCGAGCTCTCTCCGTTTCTGGAAAATTCAATCTCACTCACGCGCGTCTTCACGATCTAATCGAACGCGCCGCTTCACGCCTTGTCTCCGACGCTGGAATCAAACCCGGCGATGTCGTTGCTCTCACCTTCCCTAACACCGTCGAGTTTGTTATAATGTTTTTAGCGGTGATAAGAGCTAGAGCCACGGCGGCGCCGTTGAACGCAGCGTACACGGCGGAGGAATTTGAGTTTTACCTCTCCGATTCAGATTCAAAGCTATTGTTAACCTCTAAAGAAGGAAACGCACCGGCTCAAGAAGCAGCTTCAAAGCTGAAAATCTCTCACGTCACCGCTACGCTGCTTGACGCTGGCTCGGACCTTGTACTATCCGTTGCGGATTCAGATTCCGTCGTTGACTCAGCGACGGAACTCGTTAATCACCCGGACGACGGTGCTCTCTTCCTCCACACTTCTGGCACTACGAGCCGTCCAAAGGGTGTACCGCTTACGCAGCTCAATCTAGCTTCATCCGTCAAGAACATTAAAGCTGTGTACAAGCTTACTGAGTCTGATTCTACGGTGATTGTTCTCCCTCTGTTCCATGTTCATGGATTGTTAGCTGGGTTGCTTAGCTCGCTTGGAGCTGGTGCTGCTGTAACTCTTCCAGCTGCTGGTAGATTCTCAGCAACAACATTTTGGCCAGATATGAAGAAGTATAACGCTACATGGTATACTGCTGTGCCGACCATTCATCAGATCATATTGGACCGCCACGCGAGCCACCCTGAAACGGAATATCCTAAACTCCGGTTCATCAGGAGTTGCAGTGCTTCTTTGGCTCCGGTGATATTGTCCAGGCTTGAGGAAGCGTTTGGAGCACCGGTGCTCGAGGCCTATGCAATGACAGAGGCAACACATTTGATGAGCTCAAACCCCTTACCAGAGGAAGGTCCACACAAGCCTGGGTCTGTTGGGAAACCGGTAGGTCAAGAAATGGCGATCCTTAATGAGAAAGGCGAGATCCAAGAGCCAAATAACAAAGGAGAGGTTTGTATAAGAGGTCCAAATGTGACCAAGGGTTACAAGAATAACCCAGAAGCCAACAAGGCAGGTTTCGAGTTTGGGTGGTTCCACACTGGTGATATCGGTTACTTTGATACCGATGGGTATTTGCATCTGGTGGGTCGGATCAAAGAGCTTATTAACCGTGGAGGTGAGAAGATATCTCCAATTGAAGTGGATGCAGTACTCTTAACGCATCCTGACGTTTCTCAGGGTGTTGCATTCGGTGTTCCTGATGAGAAATATGGGGAAGAGATTAACTGTGCGGTGATTCCAAGAGAAGGAACTACTGTAACCGAAGAGGACATTAAAGCGTTTTGTAAGAAGAATTTGGCAGCTTTCAAGGTGCCAAAGAGAGTGTTCATCACTGATAACCTCCCCAAAACTGCCTCTGGTAAGATTCAGCGCCGTATCGTCGCACAACATTTCCTTGAGAAGCCCTGAGGTTCCTAAAAACCCTGGCATCTTTTCCATAACAAAAATTAAACCTCCTCCATATGAAAAATAAAAATTATAAAATGACAAGTGCCGCTACTGTTACTGCTTCGTTCAACGATACTTTTAGCGTATCCGATAATGTCGCCGTTATTGTTCCTGAAACGGACACTCAGGTGACCTACAGGGATCTATCCCACATGGTGGGTCACTTCCAGACCATGTTCACAAATCCTAATTCTCCATTGTACGGAGCTGTTTTCAGACAAGATACAGTGGCGATATCCATGCGTAATGGGCTGGAATTTATCGTCGCTTTCCTCGGTGCTACTATGGACGCTAAAATTGGCGCGCCCTTGAATCCCAATTATAAGGAAAAGGAGTTCAATTTTTATTTGAATGACCTGAAATCTAAGGCGATTTGCGTCCCAAAGGGTACCACAAAGTTACAGAGTTCTGAAATTCTAAAATCTGCCTCCACGTTTGGATGTTTTATCGTAGAGCTGGCCTTCGATGCGACCAGGTTTAGGGTAGAGTATGATATATACTCTCCAGAGGACAACTACAAAAGGGTTATTTACCGGTCTTTGAACAACGCCAAATTTGTCAACACAAATCCCGTTAAATTCCCTGGGTTTGCCCGTTCCAGTGACGTTGCCCTGATTTTGCATACCAGTGGTACCACCTCCACTCCAAAAACGGTGCCTTTGTTACATTTGAACATTGTGAGAAGCACGTTGAACATTGCTAACACTTACAAGCTAACGCCCTTGGACAGATCTTATGTCGTGATGCCTCTTTTCCACGTCCATGGGTTAATTGGTGTTTTACTTTCCACTTTTAGAACTCAGGGTTCTGTTGTGGTTCCCGATGGATTCCATCCAAAGTTATTCTGGGACCAATTTGTTAAGTACAACTGTAATTGGTTCAGTTGCGTTCCCACAATAAGCATGATTATGCTGAACATGCCCAAACCAAACCCTTTCCCACACATTAGATTCATCAGATCGTGTTCTTCTGCTTTGGCTCCAGCAACGTTCCATAAGCTGGAGAAGGAATTCAATGCACCTGTCTTGGAGGCCTATGCGATGACCGAAGCATCACATCAAATGACCTCAAACAATCTGCCTCCAGGAAAGAGAAAGCCTGGTACTGTGGGCCAGCCACAAGGAGTCACCGTCGTCATTCTAGATGACAATGACAATGTCTTGCCCCCGGGCAAAGTCGGCGAAGTTTCCATCAGAGGCGAAAACGTCACTTTGGGGTATGCTAATAATCCAAAAGCTAACAAGGAGAACTTCACCAAGAGAGAGAACTATTTCAGAACCGGTGACCAAGGTTATTTCGACCCTGAGGGGTTTTTGGTCCTTACAGGCAGAATCAAAGAGCTTATCAACAGGGGTGGTGAAAAGATTTCACCCATTGAGCTCGACGGCATTATGCTATCGCATCCAAAGATCGATGAAGCCGTTGCATTTGGTGTTCCCGACGATATGTACGGCCAAGTAGTTCAAGCCGCCATTGTTTTGAAGAAGGGAGAAAAAATGACCTACGAAGAACTGGTGAACTTCTTAAAGAAGCACCTAGCCTCTTTCAAAATTCCAACCAAGGTGTACTTTGTTGATAAGCTACCAAAAACCGCTACAGGTAAAATCCAGAGAAGAGTTATCGCAGAAACTTTTGCTAAGAGCAGCAGAAATAAGAGTAAGTTGTAGGGTTCCTAGCTGATTAGAAGGCCATCCTGACGGATGGCCTTTTTTTTGACTCTGTTGTGTCTTCTTTTAGGTGTGGAATAATTTTCCAACCATCACTTTATCTGTCTCCCGAACAGCACCTTATTCAGCCATCGGTTCACATACACGTTCACGAAAGCACATCCTACTCCGATAGCGGCTCCTGCCACTACATCACTGGGATAGTGCACCCCTTGGTTCATCCGGGCAAACCCTACTCCGCAAGCCCATACGGCTGAAGGGGCGATAACATACCACTTAGGATAAGTGATGGAGAGTGAGGTGGCTAAAGAAAAAGCTGCCGCAGTGTGTCCTGAAGGGAATGACGGACTATCGGCATCCGGTGCTCCGTATGCATGAATCTTATCCGGATACTTTACAAAAGGCCGGTCACGACCTATTATATGTTTGGCGGCGTATGTAATCCCCACTGCTTCAATCACACTGGTTCCTATGTAAATGGCATCTTTCAGCATCGGCTGGTCTTTCTTTATCAATGCATAGAGTCCCATGGCTGTAGGTATTCCTACGGGTAGAATCACTCCGCTAGCGGACAATCCGCGGCTCAGTCCGTGTACATCCCATCGGTTGATGGTTTTCAACGTGTTGATGTCTATATTCTGTGCGCTGAGGCTGAATGTAAAACAGGCTGTCAGTATAAGAAAAATAATCCTTTGGCGTTTCATTTAGTTTGGCATTTGTTCAGTGCAAAGATAGGAAAATAATGTAATTCGCATTTACCCTTCCCCGAATTAAAGAATAATTTCCATAATTCCTTGTATTATCGCTAGAATTCCTTTACTTTTGCATCGTGAATACACAGCTTTTAACAGAATTTTTAGTTCTAACAATTAAATAATTTAAATTCAATCATTTATGTGGTTAAGTAATTCATCTATTGGAAGGAAAGTGGTGATGAGTGTCACAGGTATCGCCCTTGTCCTGTTTCTGACGTTTCACATGGCGATGAACCTTGTTGCGCTCTTCTCGGAAGATGCTTACAACATGATTTGTGAATTCTTGGGAGCTAACTGGTACGCGTTGGTTGCAACTGCCGGGCTGGCTGCTCTGTTTGTAATTCACATCATCTACGCTTTCTGGTTGACCATGCAGAACCGTGCAGCTCGCGGTAGCGAACGTTATGCAGTGAACGTTAAACCTAAAACTGTGGAATGGGCATCTCAGAACATGCTTGTCCTGGGTATTGTCGTTATTGCCGGATTGGCTCTTCATTTTGCCAATTTCTGGTACAAAATGCAGTTTGCCGAAATTATCGGTGTTCATGATTTGGGCGCATTCGGTCCTACAGACGGTGCCGCTTACATTCGTGAAACATTCGGATGCCCTGTATTTACAGTGCTTTACCTCATTTGGTTAGCAGCTTTGTGGTTCCACCTGACTCACGGATTCTGGAGTGCTTTGCAGACTTTGGGCTGGAGCAATAAAATCTGGTTCGAGCGTTGGAAGACTATTGGTAACATCTACTCTACTTTGGTAGTTCTTGGTTTTGCAGCAGTAGTCGTTATCTACTTCGCTATGACTGGCGCGCCTGATAGGTGGGCTGCCCTTCCTGGTTGGCTTGGTTTCATCAGCCATCCGCTTGCCCTCATCTGTTACGCCGGCGGTAGCCGGCCAGCCTCGCAGAGCAGGATTCCCGTTGAGCACCGCCAGGTGCGAATAAGGGACAGTGAAGAAGGAACACCCGCTCGCGGGTGGGCCTACTTCACCTATCCTGCCCGGCTGACGCCGTTGGATACACCAAGGAAAGTCTACACGAACCCTTTGGCAAAATCCTGTATATCGTGCGAAAAAGGATGGATATACCGAAAAAATCGCTATAATGACCCCGAAGCAGGGTTATGCAGCGGAAAAGCGGGATTAAAAGTCGGGGATTGGTGAACAAAAAGGTGTTTCTCTCTTTAAGAGAAATATCGTTTTGCTAAACAGTTGATATTGAGGTATCATTTTATCGTAAAAGACATTTTTGCTCAACAATTGCTTGACGGAAATCAACAAATTTTAGCATTTTGTAAAAAAGTCGCTATATAATTTGGTGAATTGGAGTTATTTTCATATTTTTGCATCCCGAAGAGTTTCTCTTAAAGAGAGAAACATCTTTTGCATACCTTTTCCGACCGAATTTTTATGTCGTAAAGAGGGGCTTTGCAGGGGGTGGACTCAGAAAGATGAGAATAGATGACTATTGTAGTTGAAACACATAGAAAGTTGCTGATATACAGACCGATACGCATATCGGGATGAACCATGAGTACGTTCTTTTCTCAAAAAACATAAATATTCGAAAAGAGATGCAATAAATTAAGGAGAGGTTATAATGAACAAAGTAAATATAAAAGATAGTCAAAATTTTATTACTTCAAAATATCACATAGAAAAAATAATGAATTGCATAAGTTTAGATGAAAAAGATAACATCTTTGAAATAGGTGCAGGGAAAGGTCATTTTACTGCTGGATTGGTAAAGAGATGTAATTTTGTAACGGCGATAGAAATTGATTCTAAATTATGTGAGGTAACTCGTAATAAGCTCTTAAATTATCCTAACTATCAAATAGTAAATGATGATATACTGAAATTTACATTTCCTAGCCACAATCCATATAAAATATTTGGCAGCATACCTTACAACATAAGCACAAATATAATTCGAAAAATTGTTTTTGAAAGTTCAGCCACAATAAGTTATTTAATAGTGGAATATGGTTTTGCTAAAATGTTATTAGATACAAACAGATCACTAGCATTGCTGTTAATGGCAGAGGTAGATATTTCTATATTAGCAAAAATTCCTAGGTATTATTTCCATCCAAAACCTAAAGTGGATAGCACATTAATTGTATTAAAAAGAAAGCCAGCAAAAATGGCATTTAAAGAGAGAAAAAAATATGAAACTTTTGTAATGAAATGGGTTAACAAAGAGTACGAAAAACTGTTTACAAAAAATCAATTTAATAAAGCTTTAAAACATGCGAGAATATATGATATAAACAATATTAGTTTCGAACAATTTGTATCGCTATTTAATAGTTATAAAATATTTAACGGCTAAAAACAATAGGCCACATGCAACTGTAAATGTTTACGCGGGTACCGACACCGCGGTGGAGGGGAATTGTGTTACAACCAATTAACCAATTCTGATTAGAAAAACTCATCGAGCATCAAATGAAACTGCAATTTATTCATATCAGGATTATCAATACCATATTTTTGAAAAAGCCGTTTCTGTAATGAAGGAGAAAACTCACCGAGGCAGTTCCATAGGATGGCAAGATCCTGGTATCGGTCTGCGATTCCGACTCGTCCAACATCAATACAACCTATTAATTTCCCCTCGTCAAAAATAAGGTTATCAAGTGAGAAATCACCATGAGTGACGACTGAATCCGGTGAGAATGGCAAAAGCTTATGCATTTCTTTCCAGACTTGTTCAACAGGCCAGCCATTACGCTCGTCATCAAAATCACTCGCATCAACCAAACCGTTATTCATTCGTGATTGCGCCTGAGCGAGGCGAAATACGCGATCGCTGTTAAAAGGACAATTACAAACAGGAATCGAATGCAACCGGCGCAGGAACACTGCCAGCGCATCAACAATATTTTCACCTGAATCAGGATATTCTTCTAATACCTGGAATGCTGTTTTCCCGGGGATCGCAGTGGTGAGTAACCATGCATCATCAGGAGTACGGATAAAATGCTTGATGGTCGGAAGAGGCATAAATTCCGTCAGCCAGTTTAGTCTGACCATCTCATCTGTAACATCATTGGCAACGCTACCTTTGCCATGTTTCAGAAACAACTCTGGCGCATCGGGCTTCCCATACAATCGATAGATTGTCGCACCTGATTGCCCGACATTATCGCGAGCCCATTTATACCCATATAAATCAGCATCCATGTTGGAATTTAATCGCGGCCTGGAGCAAGACGTTTCCCGTTGAATATGGCTCATAACACCCCTTGTATTACTGTTTATGTAAGCAGACAGTTTTATTGTTCATGATGATATATTTTTATCTTGTGCAATGTAACATCAGAGATTTTGAGACACAACGTGGCTTTGTTGAATAAATCGAACTTTTGCTGAGTTGAAGGATCAGAGCCTACGTTCCGAATACGGTCAAAAAAAAGGCCATCCGTCAGGATGGCCTTCGCATTAATATGCCGCTTCGAATTCTTTTAGGAAGCGTGTATCGTTTTCAGAGAACATACGGAGGTCTTTCACCTGATATTTCAGGTTTGTGATACGCTCGATACCCATACCGAGTCCATAACCGCTGTATATTTTGCTGTCTATACCATTTGATTCAAGTACGTTCGGGTCTACCATACCGCAACCGAGGATTTCTACCCAGCCGGTGTGTTTACAGAACGGACATCCTTTACCGCCGCAGATATTACAGCTGATATCCATTTCCGCACTTGGTTCAGCAAACGGGAAGTAAGACGGACGCAGACGGATCTTTGTATCAGCACCGAACATTTCTTTGGCAAAGAGCAGCAATACCTGCTTCAAGTCGGTGAATGATACGTTTTTATCTACATACAGCGCTTCTACCTGATGGAAGAAACAGTGTGCGCGATAGCTGATAGCTTCGTTACGATATACACGTCCCGGACAGATGATGCGGATAGGAGGCTGTGAAGTTTCCATCACACGAGTCTGTACAGAAGAAGTATGTGTACGCAATACTACGTCCGGGTGAGCTTCGATAAAGAAAGTGTCCTGCATATCGCGTGCCGGATGATCTTCGGCAAAGTTCAGTGCCGAGAACACGTGCCAGTCATCTTCAATTTCCGGACCTTCGGCAATGCTGAATCCCAGACGGGCAAAGATATCAATGATTTCGTTCTTTACAATGGTGAGCGGGTGGCGTGTACCGAGTTCTACAGGATAAGCCGAACGCGTCAAATCCAGTCCGTCACAATCGTTGTCCTGACTTTCAAACATTTCTTTCAGCGCGTTGATTTTGTCCTGCGCTTTTGTTTTCAGTTCATTCAGTCTCATGCCGACTTCTTTTTTCTGTTCGGCAGCTACATTACGGAAATCTGCCATTAAGTCGTTAATGGCTCCCTTCTTACTTAGGTATTTGATGCGGAGAGCTTCGAGTTCTTCGGCATTGGAGGCGTGTAAGGCTTCCACCTCTTTCAGAAGTTGTTCAATCTTAGCTATCATTTTTTAATATTTTTAGCGGCCCCGTTAAACAAAATTATTTGTAGAGGCTGTTTCGTCCTCACGGACTCATCAGACCGGAAAGCACATCCGGTGACAGCTCAGGCTACTTTGTTTCTTTCGACACTGCAAATATAAGAACATTATTTGAAAGTTCAAGTGAAACTTTAAATTTTAACAATAGATTAACCATTGCAAACAAAACAAAAAAAAGGTAGCCCAATTGTAAAACGAAAGGCCCAGTCTTTCGACTGAGCCTTTCGTTTTATCCTACAGTCGCTCGGCGATCGAAGGCTTCGGAAAAAAAAGGCCATCCGTCAGGATGGCCTTCGCATTAATATGCCGCTTCGAATTCTTTTAGGAAGCGTGTATCGTTTTCAGAGAACATACGGAGGTCTTTCACCTGATATTTCAGGTTTGTGATACGCTCGATACCCATACCGAGTCCATAACCGCTGTATATTTTGCTGTCTATACCATTTGATTCAAGTACGTTCGGGTCTACCATACCGCAACCGAGGATTTCTACCCAGCCGGTGTGTTTACAGAACGGACATCCTTTACCGCCGCAGATATTACAGCTGATATCCATTTCCGCACTTGGTTCAGCAAACGGGAAGTAAGACGGACGCAGACGGATCTTTGTATCAGCACCGAACATTTCTTTGGCAAAGAGCAGCAATACCTGCTTCAAGTCGGTGAATGATACGTTTTTATCTACATACAGCGCTTCTACCTGATGGAAGAAACAGTGTGCGCGATAGCTGATAGCTTCGTTACGATATACACGTCCCGGACAGATGATGCGGATAGGAGGCTGTGAAGTTTCCATCACACGAGTCTGTACAGAAGAAGTATGTGTACGCAATACTACGTCCGGGTGAGCTTCGATAAAGAAAGTGTCCTGCATATCGCGTGCCGGATGATCTTCGGCAAAGTTCAGTGCCGAGAACACGTGCCAGTCATCTTCAATTTCCGGACCTTCGGCAATGCTGAATCCCAGACGGGCAAAGATATCAATGATTTCGTTCTTTACAATGGTGAGCGGGTGGCGTGTACCGAGTTCTACAGGATAAGCCGAACGCGTCAAATCCAGTCCGTCACAATCGTTGTCCTGACTTTCAAACATTTCTTTCAGCGCGTTGATTTTGTCCTGCGCTTTTGTTTTCAGTTCATTCAGTCTCATGCCGACTTCTTTTTTCTGTTCGGCAGCTACATTACGGAAATCTGCCATTAAGTCGTTAATGGCTCCCTTCTTACTTAGGTATTTGATGCGGAGAGCTTCGAGTTCTTCGGCATTGGAGGCGTGTAAGGCTTCCACCTCTTTCAGAAGTTGTTCAATCTTAGCTATCATTTTTTAATATTTTTAGCGGCCCCGTTAAACAAAATTATTTGTAGAGGCTGTTTCGTCCTCACGGACTCATCAGACCGGAAAGCACATCCGGTGACAGCTCAGGCTACTTTGTTTCTTTCGACACTGCAAATATAAGAACATTATTTGAAAGTTCAAGTGAAACTTTAAATTTTAACAATAGATTAACCATTGCAAACAAAACAAAAAAAAGGTAGCCCAATTGTAAAACGAAAGGCCCAGTCTTTCGACTGAGCCTTTCGTTTTATCCTAGGATCAGCTGTACGTACTCGCAGTTCAACCTGTTGATAGTACGTACTAAGCTCTCATGTTTCACGTACTAAGCTCTCATGTTTAACGTACTAAGCTCTCATGTTTAACGAACTAAACCCTCATGGCTAACGTACTAAGCTCTCATGGCTAACGTACTAAGCTCTCATGTTTCACGTACTAAGCTCTCATGTTTGAACAATAAAATTAATATAAATCAGCAACTTAAATAGCCTCTAAGGTTTTAAGTTTTATAAGAAAAAAAAGAATATATAAGGCTTTTAAAGCTTTTAAGGTTTAACGGTTGTGGACAACAAGCCAGGGATGTAACGCACTGAGAAGCCCTTAGAGCCTCTCAAAGCAATTTTGAGTGACACAGGAACACTTAACGGCTGACATGGGGCGGCCGCGTAG
SEQ ID NO:93(用于基因组整合进普通拟杆菌基因组中的编码草酸盐降解途径的质粒)：
GGAGAGCTGTATGTGGGGTGGAAGAAGCTATCGTAAAAATGCTGGAGCAGCTTGATCCTCATTCTACCTATTCTGATCCGGAGGAAGTGAAGAGAATGAACGAGCCGTTGCAGGGAAACTTCGAAGGTATCGGTATACAGTTTAATATGGCGGAGGACACTTTGCTGGTGATACAGCCTGTATCGGGCGGACCGTCCGAAAAAGTCGGTATTCTGGCCGGTGATCGTATCGTCATGGTGGAAGATACGCTGATAGCCGGTGTAAAGATGAGTACCGAGGATATTATGCGTCGCCTCCGTGGTCCTAAAGATTCAAAAGTGAATCTGAAAATACTTCGCCGAGGGGTGAAGGAACTGCTCCCCTTTACGGTGAAAAGGGATAAAATACCTGTTTACAGCTTGGATGCTTCCTATATGATTAAAGATAAGATCGGTTATATTCGTATCAACCGTTTTGCCGCCACTACCCATGAGGAGTTCAAGAAAGCGCTGACCGGGTTGCAGAAGAAAGGAATGAAAGACTTGATTCTTGATTTGCAGGGCAATGGCGGGGGGTATTTGAATGCTGCTATCGATATTGCCAATGAATTTTTGGGTAAAAAGGAACTGATTGTCTATACGGAGGGCCGTCGTAATCCTCGTAGCGAGTTTTTTGCCAAAGGTACGGGCGAGTTTCAGAACGGTCGTCTGATGGTATTGGTTGATGAATTTTCGGCTTCTGCCAGTGAGATTGTGACCGGAGCTGTTCAGGATTGGGACAGAGGCGTTGTGGTAGGCCGTCGCAGTTTTGGTAAAGGCTTGGTGCAGCGTCCCATTGATTTGCCGGACGGTTCGATGATCCGTCTGACGGTGGCCCGCTATTATACGCCTGCCGGACGTTGTATCCAGAAACCTTACGAGAGCATTGAAAAGTATAATGAAGACCTGATAGACCGTTATAATAAAGGAGAAATGATGAGTGAAGACAGCATCCACTTTCCGGATTCACTGAAGTTCAAGACTCATAGGTTGGCCCGTACGGTATATGGTGGCGGAGGGATCATGCCCGATTATTTTGTGCCGATTGATACGACTCTCTATACTAAATACCATCGTCAGTTGCGTGATAAAGGAGCATTGATGAAAGCTCATTTCCATTTTATTGATGCCCATCGTAAAGAATGGCTGGGCAAATACAAGACTTTCAATGAGTTCTACAAACGTTTTGAAGTGACTCCGGATATGTTGACACAACTGGTTGCTACCGGCAAGGAGATGGGAGTGGAATATAATGAAGAAGAGTATCAAAAGGCCCTGCCTCTGCTGAGGTTGCAGATGAAAGCATTGATAGCCCGTGATTTATGGGATATGAATGAATATTATCACGTCATTAATGACGCGAATGAAAGTATCAGGAAAGCGCTGGAACTGTTGGAGCAACCGGACTTTGAGGGGCTGCTGTTGAAAAAGCGATAGTCAAGACTTTATATTAACTGCAGGGGCGCATAGTTGCACTCCTGCAGTTGTCTGAAAAACTTGTAGGATAAAACGAAAGGCTCAGTCGAAAGACTGGGCCTTTCGTTTTACAATTGGGCTACCTTTTTTTTGTTTTGTTTGCAATGGTTAATCTATTGTTAAAATTTAAAGTTTCACTTGAACTTTCAAATAATGTTCTTATATTTGCAGTGTCGAAAGAAACAAAGTAGCCTGGCATCACCTAAAAATTATAAATAAATTAATAAACTTTTATTAAAAAAAAATTACAAAATGAATAATCCACAAACAGGACAATCAACAGGCCTCTTGGGCAATCGTTGGTTCTACTTGGTATTAGCAGTTTTGCTGATGTGTATGATCTCGGGTGTCCAATATTCCTGGACACTGTACGCTAACCCGGTTAAAGACAACCTTGGCGTTTCTTTGGCTGCGGTTCAGACGGCTTTCACACTCTCTCAGGTCATTCAAGCTGGTTCTCAGCCTGGTGGTGGTTACTTCGTTGATAAATTCGGTCCAAGAATTCCATTGATGTTCGGTGGTGCGATGGTTCTCGCTGGCTGGACCTTCATGGGTATGGTTGACAGTGTTCCTGCTCTGTATGCTCTTTATACTCTGGCCGGTGCAGGTGTTGGTATCGTTTACGGTATCGCGATGAACACGGCTAACAGATGGTTCCCGGACAAACGCGGTCTGGCTTCCGGTTTCACCGCTGCCGGTTACGGTCTGGGTGTTCTGCCGTTCCTGCCACTGATCAGCTCCGTTCTGAAAGTTGAAGGTGTTGGCGCAGCATTCATGTACACCGGTTTGATCATGGGTATCCTGATTATCCTGATCGCTTTCGTTATCCGTTTCCCTGGCCAGCAAGGCGCCAAAAAACAAATCGTTGTTACCGACAAGGATTTCAATTCTGGCGAAATGCTGAGAACACCACAATTCTGGGTTCTGTGGACCGCATTCTTTTCCGTTAACTTTGGTGGTTTGCTGCTGGTTGCCAACAGCGTCCCTTACGGTCGCAGCCTCGGTCTTGCCGCAGGTGTGCTGACGATCGGTGTTTCGATCCAGAACCTGTTCAATGGTGGTTGCCGTCCTTTCTGGGGTTTCGTTTCCGATAAAATCGGCCGTTACAAAACCATGTCCGTCGTTTTCGGTATCAATGCTGTTGTTCTCGCACTTTTCCCGACGATTGCTGCCTTGGGCGATGTAGCCTTTATCGCCATGTTGGCAATCGCATTCTTCACATGGGGTGGTAGCTACGCTCTGTTCCCATCGACCAACAGCGATATTTTCGGTACGGCATACTCTGCCAGAAACTATGGTTTCTTCTGGGCTGCAAAAGCAACTGCCTCGATCTTCGGTGGTGGTCTGGGTGCTGCAATTGCAACCAACTTCGGATGGAATACCGCTTTCCTGATTACTGCGATTACTTCTTTCATCGCATTTGCTCTGGCTACCTTCGTTATTCCAAGAATGGGCCGTCCAGTCAAGAAAATGGTCAAATTGTCTCCAGAAGAAAAAGCTGTACATTAAGGTTCCTAACCTGCCTGGCATCTTAATCAATTTTAAACTCACCAAATCAAAATATGAAAAAAAAAAATAATAAAATGTCAACTCCACTTCAAGGAATTAAAGTTCTCGATTTCACCGGTGTGCAATCTGGCCCATCTTGTACTCAAATGCTGGCCTGGTTTGGCGCTGACGTTATTAAAATTGAACGTCCCGGCGTTGGTGACGTAACGCGTCACCAGCTGCGAGATATTCCTGATATCGATGCGCTTTACTTCACCATGCTTAACAGTAACAAACGTTCTATTGAGTTAAATACCAAAACAGCGGAAGGCAAAGAGGTAATGGAAAAGCTGATCCGCGAAGCTGATATCTTAGTCGAGAACTTTCATCCAGGGGCCATTGATCACATGGGCTTCACCTGGGAGCATATTCAAGAAATCAATCCACGTCTGATTTTTGGTTCGATCAAAGGGTTTGATGAGTGTTCGCCTTATGTGAATGTAAAAGCCTATGAAAACGTTGCTCAGGCAGCGGGTGGCGCGGCATCCACTACGGGTTTTTGGGATGGTCCGCCGCTGGTAAGCGCTGCAGCGTTGGGTGACAGCAACACCGGAATGCATTTGCTGATCGGTTTACTTGCTGCTTTGCTGCATCGCGAAAAAACGGGGCGTGGGCAACGAGTCACCATGTCAATGCAGGATGCCGTATTGAACCTTTGCCGCGTGAAATTACGTGACCAGCAGCGTCTAGATAAATTGGGTTATCTGGAAGAATACCCGCAGTATCCGAATGGTACATTTGGTGATGCAGTTCCCCGCGGTGGTAATGCAGGTGGTGGCGGTCAGCCTGGCTGGATCCTGAAATGTAAAGGCTGGGAAACCGATCCTAACGCCTATATTTATTTCACTATTCAGGAGCAAAACTGGGAAAACACCTGTAAAGCCATCGGCAAACCAGAATGGATTACCGATCCGGCATACAGTACAGCCCATGCACGACAGCCACATATTTTCGATATTTTTGCTGAAATCGAAAAATACACTGTCACTATTGATAAACATGAAGCGGTGGCCTATTTGACTCAGTTTGATATTCCTTGTGCACCGGTTTTAAGTATGAAAGAAATTTCACTTGATCCCTCTTTGCGCCAAAGTGGCAGTGTTGTTGAAGTGGAACAACCGTTGCGTGGAAAATATCTGACCGTTGGTTGTCCAATGAAATTCTCTGCCTTTACGCCGGATATTAAAGCTGCGCCGCTATTAGGTGAACATACCGCTGCTGTATTGCAGGAGCTGGGTTATAGCGACGATGAAATTGCTGCAATGAAGCAAAACCACGCCATCTGAGGTTCCTAAACGACCTGGCATCAACTACCTTTATTATTCAACAAACCAATTTAACATATTAAAAAAATAAAAAAATGACTACCACAGCAACGCAGCATTTTGCCCAGTTACTCCAGAAGTACGGAATTGATACTGTCTTCGGCATAGTAGGTATTCCGATCGTGCAGTTGGCCGACACTATGGTCGCGAATGGAATTAAGTTTATACCGTGTCGCAATGAGCAAGCCGCAAGTTACGCGGCAAGCGCCTACGGCTACATCTCGGACAAACCTGGTGTCCTGTTAATCGTGGGAGGGCCTGGCCTTATTCATGCCTTAGCGGGGATCTATAATAGTATGTCGAACCGCTGGCCCTTGTTGGTCATCGCCGGATCCAGCAGCCAGTCAGACATCCACAAGGGAGGTTTCCAGGAACTTGACCAGGTGTCCTTGCTAAGTCCGTTCCTAAAATTCACTGGTAAACTGACGCCGGATAACATCGATATGATAACCCAAAAAGCACTCAACTATTGCATTCAGGGAACCGCCGGAGTGAGCTATATCGATGTCCCCGCTGACTTCATCGAATACGAGAAGCCGCTTGAGGGCAATGATAGAACTGGTAACGAGCTGCCAATGATCCTGACGCCCAATATCTGCGGTCCAGACCCCAGTAAAATTAAGAAGGTGGTGCAATTGATACTCCAGCACAAGAACAAGAACATATTAATAGTGATAGGTAAAGGAGCAGTGAAGAATAGCCATGAGATTAGACGCTTGGTTAACACCTTTAACTTGCCGTTCCTTCCGACTCCCATGGCGAAAGGCATAGTGCCCGATAGCAGCCCTTTAAATGTGAGCAGCGCCCGGAGCCAGGCTCTTAAGATTGCTGACATCGTGTTAGTATTGGGAGCTCGCCTGAATTGGATCCTTCACTTCGGAACGAGTCCAAAGTGGAATAGCGAGAGTATTTTTATACAATTTGACAGTAACCCGGAAACGCTCGGAGACAATAACGTTAGTCCCGGAGCAGATTTAAGTATCTGGGGGGACATCGGACTATCGGTTACTGCACTGGTAGAAGAGCTCACGAGACAGGACAGTTGTTGGAAATACTCGGGAGTCAAACAGGAGATCAGGGAAAAGATACAGTTAAACCAGACCAGATTGCTCCGTAAGGAAAAAACGCGTGGAGCTCAGCTTAACTATAATCAAGTGTACGGCACCCTTCGTCCGTTAATTGACGACTATCGCACGATCTTGGTCACAGAAGGTGCCAACACAATGGACATCGCTAGAATAAGTTTTCCGACTGATGCACCTCGCAGAAGACTTGATGCTGGTACAAATGCTACAATGGGTATAGGTTTAGGTTACGCATTAGCGTGTAAAGCCAGCCACCCTGAGTTAGACGTTGTTTTAATCCAAGGCGACAGTGCCTTCGGTTTTAGCGCTATGGAGATCGAGACTGCCGTCCGTTGCCAATTAGCCCTTGTGATTGTCGTAATGAATAACTCTGGGATATATCACGGTGAGAAAGACATCGAAGGAGATCTGCCCCCGACCGCTTTGTCAAAAAATTGTAGATATGACTTGGTCGGTAAGGGTTTAGGAGCTAACGACTTCTTCGTCAATACCATCTCAGAGTTATCTCGCTGTTTTCAGCAGGCAGTTCAATTGAGCAGAACCAAGCGCGAAACCTCCGTCATTAATGTCATAATCGAACCGGGAGAGCAGAAACAGATCGCGTTCGCATGGCAAAACAAGCCTAGACTGTAAGGTTCCTAAGGGTCCTGGCATCAACTACCTAAATTACTTATCAAACCACTTAGTAGTAAAAAAAATATAACAAAATGACAAATAATGAAAGCAAAGGGCCGTTTGAAGGCTTATTAGTTATCGATATGACACATGTCCTTAATGGACCTTTCGGAACTCAACTTCTTTGTAATATGGGCGCAAGGGTAATTAAAGTTGAGCCGCCGGGTCATGGTGATGATACCCGCACATTTGGTCCCTATGTGGATGGACAGTCACTCTATTACAGTTTTATTAATCATGGCAAAGAGAGTGTGGTTCTTGATTTAAAGAATGATCACGATAAAAGTATATTTATAAATATGCTCAAACAAGCTGATGTATTAGCTGAGAATTTTCGCCCAGGTACAATGGAAAAACTGGGGTTTTCATGGGAAACGCTTCAAGAAATCAACCCGCGCCTCATATATGCTTCATCGTCAGGTTTCGGACATACCGGTCCGCTAAAAGATGCTCCTGCCTACGATACCATCATTCAGGCAATGAGCGGGATAATGATGGAAACAGGATATCCTGATGCTCCGCCAGTGCGCGTTGGTACATCTCTTGCGGATCTATGCGGCGGTGTCTATTTATTCAGCGGAATAGTGAGTGCACTTTATGGCCGCGAAAAGAGCCAGAGAGGGGCGCATGTCGATATAGCGATGTTTGATGCCACGCTGAGTTTTCTGGAGCATGGTCTGATGGCATATATCGCAACTGGGAAGTCACCACAACGTCTGGGAAATCGCCATCCCTACATGGCACCTTTTGATGTTTTCAATACTCAGGATAAGCCGATTACGATTTGTTGTGGTAATGACAAGCTTTTTTCTGCGTTATGCCAGGCACTGGAGCTTACGGAACTGGTTAATGATCCCCGATTTAGCAGCAATATTTTACGCGTACAAAACCAGGCTATTCTTAAACAATATATTGAGCGGACGTTAAAAACGCAGGCAGCTGAAGTTTGGTTAGCCAGAATACATGAAGTTGGTGTACCCGTCGCGCCGTTATTAAGTGTGGCTGAGGCCATTAAATTGCCACAAACTCAGGCGAGAAATATGTTGATTGAAGCCGGGGGAATAATGATGCCGGGTAATCCGATAAAAATCAGCGGCTGCGCGGACCCGCATGTTATGCCGGGAGCGGCAACGCTCGACCAGCATGGGGAACAAATTCGCCAGGAGTTCTCATCATAAGGTTCCTAATTCCCCTGGCATCTTTTCCATAACAAAAATTAAACCTCCTCCATATGAAAAATAAAAATTATAAAATGACAAGTGCCGCTACTGTTACTGCTTCGTTCAACGATACTTTTAGCGTATCCGATAATGTCGCCGTTATTGTTCCTGAAACGGACACTCAGGTGACCTACAGGGATCTATCCCACATGGTGGGTCACTTCCAGACCATGTTCACAAATCCTAATTCTCCATTGTACGGAGCTGTTTTCAGACAAGATACAGTGGCGATATCCATGCGTAATGGGCTGGAATTTATCGTCGCTTTCCTCGGTGCTACTATGGACGCTAAAATTGGCGCGCCCTTGAATCCCAATTATAAGGAAAAGGAGTTCAATTTTTATTTGAATGACCTGAAATCTAAGGCGATTTGCGTCCCAAAGGGTACCACAAAGTTACAGAGTTCTGAAATTCTAAAATCTGCCTCCACGTTTGGATGTTTTATCGTAGAGCTGGCCTTCGATGCGACCAGGTTTAGGGTAGAGTATGATATATACTCTCCAGAGGACAACTACAAAAGGGTTATTTACCGGTCTTTGAACAACGCCAAATTTGTCAACACAAATCCCGTTAAATTCCCTGGGTTTGCCCGTTCCAGTGACGTTGCCCTGATTTTGCATACCAGTGGTACCACCTCCACTCCAAAAACGGTGCCTTTGTTACATTTGAACATTGTGAGAAGCACGTTGAACATTGCTAACACTTACAAGCTAACGCCCTTGGACAGATCTTATGTCGTGATGCCTCTTTTCCACGTCCATGGGTTAATTGGTGTTTTACTTTCCACTTTTAGAACTCAGGGTTCTGTTGTGGTTCCCGATGGATTCCATCCAAAGTTATTCTGGGACCAATTTGTTAAGTACAACTGTAATTGGTTCAGTTGCGTTCCCACAATAAGCATGATTATGCTGAACATGCCCAAACCAAACCCTTTCCCACACATTAGATTCATCAGATCGTGTTCTTCTGCTTTGGCTCCAGCAACGTTCCATAAGCTGGAGAAGGAATTCAATGCACCTGTCTTGGAGGCCTATGCGATGACCGAAGCATCACATCAAATGACCTCAAACAATCTGCCTCCAGGAAAGAGAAAGCCTGGTACTGTGGGCCAGCCACAAGGAGTCACCGTCGTCATTCTAGATGACAATGACAATGTCTTGCCCCCGGGCAAAGTCGGCGAAGTTTCCATCAGAGGCGAAAACGTCACTTTGGGGTATGCTAATAATCCAAAAGCTAACAAGGAGAACTTCACCAAGAGAGAGAACTATTTCAGAACCGGTGACCAAGGTTATTTCGACCCTGAGGGGTTTTTGGTCCTTACAGGCAGAATCAAAGAGCTTATCAACAGGGGTGGTGAAAAGATTTCACCCATTGAGCTCGACGGCATTATGCTATCGCATCCAAAGATCGATGAAGCCGTTGCATTTGGTGTTCCCGACGATATGTACGGCCAAGTAGTTCAAGCCGCCATTGTTTTGAAGAAGGGAGAAAAAATGACCTACGAAGAACTGGTGAACTTCTTAAAGAAGCACCTAGCCTCTTTCAAAATTCCAACCAAGGTGTACTTTGTTGATAAGCTACCAAAAACCGCTACAGGTAAAATCCAGAGAAGAGTTATCGCAGAAACTTTTGCTAAGAGCAGCAGAAATAAGAGTAAGTTGTAGGGTTCCTAAAATGCCTGGCATCAACTACCTAAATTAATTATCACCCCAAATTAATAACATAAAAATATAATAAAATGAGTAACGACGACAATGTAGAGTTGACTGATGGCTTTCATGTTTTGATCGATGCCCTGAAAATGAATGACATCGATACCATGTATGGTGTTGTCGGCATTCCTATCACGAACCTGGCTCGTATGTGGCAAGATGACGGTCAGCGTTTTTACAGCTTCCGTCACGAACAACACGCAGGTTATGCAGCTTCTATCGCCGGTTACATCGAAGGAAAACCTGGCGTTTGCTTGACCGTTTCCGCCCCTGGCTTCCTGAACGGCGTGACTTCCCTGGCTCATGCAACCACCAACTGCTTCCCAATGATCCTGTTGAGCGGTTCCAGTGAACGTGAAATCGTCGATTTGCAACAGGGCGATTACGAAGAAATGGATCAGATGAATGTTGCACGTCCACACTGCAAAGCTTCTTTCCGTATCAACAGCATCAAAGACATTCCAATCGGTATCGCTCGTGCAGTTCGCACCGCTGTATCCGGACGTCCAGGTGGTGTTTACGTTGACTTGCCAGCAAAACTGTTCGGTCAGACCATTTCTGTAGAAGAAGCTAACAAACTGCTCTTCAAACCAATCGATCCAGCTCCGGCACAGATTCCTGCTGAAGATGCTATCGCTCGCGCTGCTGACCTGATCAAGAACGCCAAACGTCCAGTTATCATGCTGGGTAAAGGCGCTGCATACGCACAATGCGACGACGAAATCCGCGCACTGGTTGAAGAAACCGGCATCCCATTCCTGCCAATGGGTATGGCTAAAGGCCTGCTGCCTGACAACCATCCACAATCCGCTGCTGCAACCCGTGCTTTCGCACTGGCACAGTGTGACGTTTGCGTACTGATCGGCGCTCGTCTGAACTGGCTGATGCAGCACGGTAAAGGCAAAACCTGGGGCGACGAACTGAAGAAATACGTTCAGATCGACATCCAGGCTAACGAAATGGACAGCAACCAGCCTATCGCTGCACCAGTTGTTGGTGACATCAAGTCCGCCGTTTCCCTGCTCCGCAAAGCACTGAAAGGCGCTCCAAAAGCTGACGCTGAATGGACCGGCGCTCTGAAAGCCAAAGTTGACGGCAACAAAGCCAAACTGGCTGGCAAGATGACTGCCGAAACCCCATCCGGAATGATGAACTACTCCAATTCCCTGGGCGTTGTTCGTGACTTCATGCTGGCAAATCCGGATATTTCCCTGGTTAACGAAGGCGCTAATGCACTCGACAACACTCGTATGATTGTTGACATGCTGAAACCACGCAAACGTCTTGACTCCGGTACCTGGGGTGTTATGGGTATTGGTATGGGCTACTGCGTTGCTGCAGCTGCTGTTACCGGCAAACCGGTTATCGCTGTTGAAGGCGATAGCGCATTCGGTTTCTCCGGTATGGAACTGGAAACCATCTGCCGTTACAACCTGCCAGTTACCGTTATCATCATGAACAATGGTGGTATCTATAAAGGTAACGAAGCAGATCCACAACCAGGCGTTATCTCCTGTACCCGTCTGACCCGTGGTCGTTACGACATGATGATGGAAGCATTTGGCGGTAAAGGTTATGTTGCCAATACTCCAGCAGAACTGAAAGCTGCTCTGGAAGAAGCTGTTGCTTCCGGCAAACCATGCCTGATCAACGCGATGATCGATCCAGACGCTGGTGTCGAATCTGGCCGTATCAAGAGCCTGAACGTTGTAAGTAAAGTTGGCAAGAAATAAGGTTCCTAAAAACCCTGGCATCTAAACTAAAATACAATAACCAAAAATCATTACAAATGAAAAAAAATAATAAAATGGATAGCGATACTCTCTCAGGATTATTGGAAAACGTCGCCAAAAAATTCCCCGATCGCCGAGCTCTCTCCGTTTCTGGAAAATTCAATCTCACTCACGCGCGTCTTCACGATCTAATCGAACGCGCCGCTTCACGCCTTGTCTCCGACGCTGGAATCAAACCCGGCGATGTCGTTGCTCTCACCTTCCCTAACACCGTCGAGTTTGTTATAATGTTTTTAGCGGTGATAAGAGCTAGAGCCACGGCGGCGCCGTTGAACGCAGCGTACACGGCGGAGGAATTTGAGTTTTACCTCTCCGATTCAGATTCAAAGCTATTGTTAACCTCTAAAGAAGGAAACGCACCGGCTCAAGAAGCAGCTTCAAAGCTGAAAATCTCTCACGTCACCGCTACGCTGCTTGACGCTGGCTCGGACCTTGTACTATCCGTTGCGGATTCAGATTCCGTCGTTGACTCAGCGACGGAACTCGTTAATCACCCGGACGACGGTGCTCTCTTCCTCCACACTTCTGGCACTACGAGCCGTCCAAAGGGTGTACCGCTTACGCAGCTCAATCTAGCTTCATCCGTCAAGAACATTAAAGCTGTGTACAAGCTTACTGAGTCTGATTCTACGGTGATTGTTCTCCCTCTGTTCCATGTTCATGGATTGTTAGCTGGGTTGCTTAGCTCGCTTGGAGCTGGTGCTGCTGTAACTCTTCCAGCTGCTGGTAGATTCTCAGCAACAACATTTTGGCCAGATATGAAGAAGTATAACGCTACATGGTATACTGCTGTGCCGACCATTCATCAGATCATATTGGACCGCCACGCGAGCCACCCTGAAACGGAATATCCTAAACTCCGGTTCATCAGGAGTTGCAGTGCTTCTTTGGCTCCGGTGATATTGTCCAGGCTTGAGGAAGCGTTTGGAGCACCGGTGCTCGAGGCCTATGCAATGACAGAGGCAACACATTTGATGAGCTCAAACCCCTTACCAGAGGAAGGTCCACACAAGCCTGGGTCTGTTGGGAAACCGGTAGGTCAAGAAATGGCGATCCTTAATGAGAAAGGCGAGATCCAAGAGCCAAATAACAAAGGAGAGGTTTGTATAAGAGGTCCAAATGTGACCAAGGGTTACAAGAATAACCCAGAAGCCAACAAGGCAGGTTTCGAGTTTGGGTGGTTCCACACTGGTGATATCGGTTACTTTGATACCGATGGGTATTTGCATCTGGTGGGTCGGATCAAAGAGCTTATTAACCGTGGAGGTGAGAAGATATCTCCAATTGAAGTGGATGCAGTACTCTTAACGCATCCTGACGTTTCTCAGGGTGTTGCATTCGGTGTTCCTGATGAGAAATATGGGGAAGAGATTAACTGTGCGGTGATTCCAAGAGAAGGAACTACTGTAACCGAAGAGGACATTAAAGCGTTTTGTAAGAAGAATTTGGCAGCTTTCAAGGTGCCAAAGAGAGTGTTCATCACTGATAACCTCCCCAAAACTGCCTCTGGTAAGATTCAGCGCCGTATCGTCGCACAACATTTCCTTGAGAAGCCCTGAGGTTCCTAGCTGATTAGAAGGCCATCCTGACGGATGGCCTTTTTTTTGACTCTGTTGTGTCTTCTTTTAGGTGTGGAATAATTTTCCAACCATCACTTTATCTGTCTCCCGAACAGCACCTTATTCAGCCATCGGTTCACATACACGTTCACGAAAGCACATCCTACTCCGATAGCGGCTCCTGCCACTACATCACTGGGATAGTGCACCCCTTGGTTCATCCGGGCAAACCCTACTCCGCAAGCCCATACGGCTGAAGGGGCGATAACATACCACTTAGGATAAGTGATGGAGAGTGAGGTGGCTAAAGAAAAAGCTGCCGCAGTGTGTCCTGAAGGGAATGACGGACTATCGGCATCCGGTGCTCCGTATGCATGAATCTTATCCGGATACTTTACAAAAGGCCGGTCACGACCTATTATATGTTTGGCGGCGTATGTAATCCCCACTGCTTCAATCACACTGGTTCCTATGTAAATGGCATCTTTCAGCATCGGCTGGTCTTTCTTTATCAATGCATAGAGTCCCATGGCTGTAGGTATTCCTACGGGTAGAATCACTCCGCTAGCGGACAATCCGCGGCTCAGTCCGTGTACATCCCATCGGTTGATGGTTTTCAACGTGTTGATGTCTATATTCTGTGCGCTGAGGCTGAATGTAAAACAGGCTGTCAGTATAAGAAAAATAATCCTTTGGCGTTTCATTTAGTTTGGCATTTGTTCAGTGCAAAGATAGGAAAATAATGTAATTCGCATTTACCCTTCCCCGAATTAAAGAATAATTTCCATAATTCCTTGTATTATCGCTAGAATTCCTTTACTTTTGCATCGTGAATACACAGCTTTTAACAGAATTTTTAGTTCTAACAATTAAATAATTTAAATTCAATCATTTATGTGGTTAAGTAATTCATCTATTGGAAGGAAAGTGGTGATGAGTGTCACAGGTATCGCCCTTGTCCTGTTTCTGACGTTTCACATGGCGATGAACCTTGTTGCGCTCTTCTCGGAAGATGCTTACAACATGATTTGTGAATTCTTGGGAGCTAACTGGTACGCGTTGGTTGCAACTGCCGGGCTGGCTGCTCTGTTTGTAATTCACATCATCTACGCTTTCTGGTTGACCATGCAGAACCGTGCAGCTCGCGGTAGCGAACGTTATGCAGTGAACGTTAAACCTAAAACTGTGGAATGGGCATCTCAGAACATGCTTGTCCTGGGTATTGTCGTTATTGCCGGATTGGCTCTTCATTTTGCCAATTTCTGGTACAAAATGCAGTTTGCCGAAATTATCGGTGTTCATGATTTGGGCGCATTCGGTCCTACAGACGGTGCCGCTTACATTCGTGAAACATTCGGATGCCCTGTATTTACAGTGCTTTACCTCATTTGGTTAGCAGCTTTGTGGTTCCACCTGACTCACGGATTCTGGAGTGCTTTGCAGACTTTGGGCTGGAGCAATAAAATCTGGTTCGAGCGTTGGAAGACTATTGGTAACATCTACTCTACTTTGGTAGTTCTTGGTTTTGCAGCAGTAGTCGTTATCTACTTCGCTATGACTGGCGCGCCTGATAGGTGGGCTGCCCTTCCTGGTTGGCTTGGTTTCATCAGCCATCCGCTTGCCCTCATCTGTTACGCCGGCGGTAGCCGGCCAGCCTCGCAGAGCAGGATTCCCGTTGAGCACCGCCAGGTGCGAATAAGGGACAGTGAAGAAGGAACACCCGCTCGCGGGTGGGCCTACTTCACCTATCCTGCCCGGCTGACGCCGTTGGATACACCAAGGAAAGTCTACACGAACCCTTTGGCAAAATCCTGTATATCGTGCGAAAAAGGATGGATATACCGAAAAAATCGCTATAATGACCCCGAAGCAGGGTTATGCAGCGGAAAAGCGGGATTAAAAGTCGGGGATTGGTGAACAAAAAGGTGTTTCTCTCTTTAAGAGAAATATCGTTTTGCTAAACAGTTGATATTGAGGTATCATTTTATCGTAAAAGACATTTTTGCTCAACAATTGCTTGACGGAAATCAACAAATTTTAGCATTTTGTAAAAAAGTCGCTATATAATTTGGTGAATTGGAGTTATTTTCATATTTTTGCATCCCGAAGAGTTTCTCTTAAAGAGAGAAACATCTTTTGCATACCTTTTCCGACCGAATTTTTATGTCGTAAAGAGGGGCTTTGCAGGGGGTGGACTCAGAAAGATGAGAATAGATGACTATTGTAGTTGAAACACATAGAAAGTTGCTGATATACAGACCGATACGCATATCGGGATGAACCATGAGTACGTTCTTTTCTCAAAAAACATAAATATTCGAAAAGAGATGCAATAAATTAAGGAGAGGTTATAATGAACAAAGTAAATATAAAAGATAGTCAAAATTTTATTACTTCAAAATATCACATAGAAAAAATAATGAATTGCATAAGTTTAGATGAAAAAGATAACATCTTTGAAATAGGTGCAGGGAAAGGTCATTTTACTGCTGGATTGGTAAAGAGATGTAATTTTGTAACGGCGATAGAAATTGATTCTAAATTATGTGAGGTAACTCGTAATAAGCTCTTAAATTATCCTAACTATCAAATAGTAAATGATGATATACTGAAATTTACATTTCCTAGCCACAATCCATATAAAATATTTGGCAGCATACCTTACAACATAAGCACAAATATAATTCGAAAAATTGTTTTTGAAAGTTCAGCCACAATAAGTTATTTAATAGTGGAATATGGTTTTGCTAAAATGTTATTAGATACAAACAGATCACTAGCATTGCTGTTAATGGCAGAGGTAGATATTTCTATATTAGCAAAAATTCCTAGGTATTATTTCCATCCAAAACCTAAAGTGGATAGCACATTAATTGTATTAAAAAGAAAGCCAGCAAAAATGGCATTTAAAGAGAGAAAAAAATATGAAACTTTTGTAATGAAATGGGTTAACAAAGAGTACGAAAAACTGTTTACAAAAAATCAATTTAATAAAGCTTTAAAACATGCGAGAATATATGATATAAACAATATTAGTTTCGAACAATTTGTATCGCTATTTAATAGTTATAAAATATTTAACGGCTAAAAACAATAGGCCACATGCAACTGTAAATGTTTACGCGGGTACCGACACCGCGGTGGAGGGGAATTGTGTTACAACCAATTAACCAATTCTGATTAGAAAAACTCATCGAGCATCAAATGAAACTGCAATTTATTCATATCAGGATTATCAATACCATATTTTTGAAAAAGCCGTTTCTGTAATGAAGGAGAAAACTCACCGAGGCAGTTCCATAGGATGGCAAGATCCTGGTATCGGTCTGCGATTCCGACTCGTCCAACATCAATACAACCTATTAATTTCCCCTCGTCAAAAATAAGGTTATCAAGTGAGAAATCACCATGAGTGACGACTGAATCCGGTGAGAATGGCAAAAGCTTATGCATTTCTTTCCAGACTTGTTCAACAGGCCAGCCATTACGCTCGTCATCAAAATCACTCGCATCAACCAAACCGTTATTCATTCGTGATTGCGCCTGAGCGAGGCGAAATACGCGATCGCTGTTAAAAGGACAATTACAAACAGGAATCGAATGCAACCGGCGCAGGAACACTGCCAGCGCATCAACAATATTTTCACCTGAATCAGGATATTCTTCTAATACCTGGAATGCTGTTTTCCCGGGGATCGCAGTGGTGAGTAACCATGCATCATCAGGAGTACGGATAAAATGCTTGATGGTCGGAAGAGGCATAAATTCCGTCAGCCAGTTTAGTCTGACCATCTCATCTGTAACATCATTGGCAACGCTACCTTTGCCATGTTTCAGAAACAACTCTGGCGCATCGGGCTTCCCATACAATCGATAGATTGTCGCACCTGATTGCCCGACATTATCGCGAGCCCATTTATACCCATATAAATCAGCATCCATGTTGGAATTTAATCGCGGCCTGGAGCAAGACGTTTCCCGTTGAATATGGCTCATAACACCCCTTGTATTACTGTTTATGTAAGCAGACAGTTTTATTGTTCATGATGATATATTTTTATCTTGTGCAATGTAACATCAGAGATTTTGAGACACAACGTGGCTTTGTTGAATAAATCGAACTTTTGCTGAGTTGAAGGATCAGAGCCTACGTTCCGAATACGGTCAAAAAAAAGGCCATCCGTCAGGATGGCCTTCGCATTAATATGCCGCTTCGAATTCTTTTAGGAAGCGTGTATCGTTTTCAGAGAACATACGGAGGTCTTTCACCTGATATTTCAGGTTTGTGATACGCTCGATACCCATACCGAGTCCATAACCGCTGTATATTTTGCTGTCTATACCATTTGATTCAAGTACGTTCGGGTCTACCATACCGCAACCGAGGATTTCTACCCAGCCGGTGTGTTTACAGAACGGACATCCTTTACCGCCGCAGATATTACAGCTGATATCCATTTCCGCACTTGGTTCAGCAAACGGGAAGTAAGACGGACGCAGACGGATCTTTGTATCAGCACCGAACATTTCTTTGGCAAAGAGCAGCAATACCTGCTTCAAGTCGGTGAATGATACGTTTTTATCTACATACAGCGCTTCTACCTGATGGAAGAAACAGTGTGCGCGATAGCTGATAGCTTCGTTACGATATACACGTCCCGGACAGATGATGCGGATAGGAGGCTGTGAAGTTTCCATCACACGAGTCTGTACAGAAGAAGTATGTGTACGCAATACTACGTCCGGGTGAGCTTCGATAAAGAAAGTGTCCTGCATATCGCGTGCCGGATGATCTTCGGCAAAGTTCAGTGCCGAGAACACGTGCCAGTCATCTTCAATTTCCGGACCTTCGGCAATGCTGAATCCCAGACGGGCAAAGATATCAATGATTTCGTTCTTTACAATGGTGAGCGGGTGGCGTGTACCGAGTTCTACAGGATAAGCCGAACGCGTCAAATCCAGTCCGTCACAATCGTTGTCCTGACTTTCAAACATTTCTTTCAGCGCGTTGATTTTGTCCTGCGCTTTTGTTTTCAGTTCATTCAGTCTCATGCCGACTTCTTTTTTCTGTTCGGCAGCTACATTACGGAAATCTGCCATTAAGTCGTTAATGGCTCCCTTCTTACTTAGGTATTTGATGCGGAGAGCTTCGAGTTCTTCGGCATTGGAGGCGTGTAAGGCTTCCACCTCTTTCAGAAGTTGTTCAATCTTAGCTATCATTTTTTAATATTTTTAGCGGCCCCGTTAAACAAAATTATTTGTAGAGGCTGTTTCGTCCTCACGGACTCATCAGACCGGAAAGCACATCCGGTGACAGCTCAGGCTACTTTGTTTCTTTCGACACTGCAAATATAAGAACATTATTTGAAAGTTCAAGTGAAACTTTAAATTTTAACAATAGATTAACCATTGCAAACAAAACAAAAAAAAGGTAGCCCAATTGTAAAACGAAAGGCCCAGTCTTTCGACTGAGCCTTTCGTTTTATCCTACAGTCGCTCGGCGATCGAAGGCTTCGGAAAAAAAAGGCCATCCGTCAGGATGGCCTTCGCATTAATATGCCGCTTCGAATTCTTTTAGGAAGCGTGTATCGTTTTCAGAGAACATACGGAGGTCTTTCACCTGATATTTCAGGTTTGTGATACGCTCGATACCCATACCGAGTCCATAACCGCTGTATATTTTGCTGTCTATACCATTTGATTCAAGTACGTTCGGGTCTACCATACCGCAACCGAGGATTTCTACCCAGCCGGTGTGTTTACAGAACGGACATCCTTTACCGCCGCAGATATTACAGCTGATATCCATTTCCGCACTTGGTTCAGCAAACGGGAAGTAAGACGGACGCAGACGGATCTTTGTATCAGCACCGAACATTTCTTTGGCAAAGAGCAGCAATACCTGCTTCAAGTCGGTGAATGATACGTTTTTATCTACATACAGCGCTTCTACCTGATGGAAGAAACAGTGTGCGCGATAGCTGATAGCTTCGTTACGATATACACGTCCCGGACAGATGATGCGGATAGGAGGCTGTGAAGTTTCCATCACACGAGTCTGTACAGAAGAAGTATGTGTACGCAATACTACGTCCGGGTGAGCTTCGATAAAGAAAGTGTCCTGCATATCGCGTGCCGGATGATCTTCGGCAAAGTTCAGTGCCGAGAACACGTGCCAGTCATCTTCAATTTCCGGACCTTCGGCAATGCTGAATCCCAGACGGGCAAAGATATCAATGATTTCGTTCTTTACAATGGTGAGCGGGTGGCGTGTACCGAGTTCTACAGGATAAGCCGAACGCGTCAAATCCAGTCCGTCACAATCGTTGTCCTGACTTTCAAACATTTCTTTCAGCGCGTTGATTTTGTCCTGCGCTTTTGTTTTCAGTTCATTCAGTCTCATGCCGACTTCTTTTTTCTGTTCGGCAGCTACATTACGGAAATCTGCCATTAAGTCGTTAATGGCTCCCTTCTTACTTAGGTATTTGATGCGGAGAGCTTCGAGTTCTTCGGCATTGGAGGCGTGTAAGGCTTCCACCTCTTTCAGAAGTTGTTCAATCTTAGCTATCATTTTTTAATATTTTTAGCGGCCCCGTTAAACAAAATTATTTGTAGAGGCTGTTTCGTCCTCACGGACTCATCAGACCGGAAAGCACATCCGGTGACAGCTCAGGCTACTTTGTTTCTTTCGACACTGCAAATATAAGAACATTATTTGAAAGTTCAAGTGAAACTTTAAATTTTAACAATAGATTAACCATTGCAAACAAAACAAAAAAAAGGTAGCCCAATTGTAAAACGAAAGGCCCAGTCTTTCGACTGAGCCTTTCGTTTTATCCTAGGATCAGCTGTACGTACTCGCAGTTCAACCTGTTGATAGTACGTACTAAGCTCTCATGTTTCACGTACTAAGCTCTCATGTTTAACGTACTAAGCTCTCATGTTTAACGAACTAAACCCTCATGGCTAACGTACTAAGCTCTCATGGCTAACGTACTAAGCTCTCATGTTTCACGTACTAAGCTCTCATGTTTGAACAATAAAATTAATATAAATCAGCAACTTAAATAGCCTCTAAGGTTTTAAGTTTTATAAGAAAAAAAAGAATATATAAGGCTTTTAAAGCTTTTAAGGTTTAACGGTTGTGGACAACAAGCCAGGGATGTAACGCACTGAGAAGCCCTTAGAGCCTCTCAAAGCAATTTTGAGTGACACAGGAACACTTAACGGCTGACATGGGGCGGCCGCGTAG
SEQ ID NO:94(核糖体结合位点和At_AAE3开放读码框)：
GGAGAGCTGTATGTGGGGTGGAAGAAGCTATCGTAAAAATGCTGGAGCAGCTTGATCCTCATTCTACCTATTCTGATCCGGAGGAAGTGAAGAGAATGAACGAGCCGTTGCAGGGAAACTTCGAAGGTATCGGTATACAGTTTAATATGGCGGAGGACACTTTGCTGGTGATACAGCCTGTATCGGGCGGACCGTCCGAAAAAGTCGGTATTCTGGCCGGTGATCGTATCGTCATGGTGGAAGATACGCTGATAGCCGGTGTAAAGATGAGTACCGAGGATATTATGCGTCGCCTCCGTGGTCCTAAAGATTCAAAAGTGAATCTGAAAATACTTCGCCGAGGGGTGAAGGAACTGCTCCCCTTTACGGTGAAAAGGGATAAAATACCTGTTTACAGCTTGGATGCTTCCTATATGATTAAAGATAAGATCGGTTATATTCGTATCAACCGTTTTGCCGCCACTACCCATGAGGAGTTCAAGAAAGCGCTGACCGGGTTGCAGAAGAAAGGAATGAAAGACTTGATTCTTGATTTGCAGGGCAATGGCGGGGGGTATTTGAATGCTGCTATCGATATTGCCAATGAATTTTTGGGTAAAAAGGAACTGATTGTCTATACGGAGGGCCGTCGTAATCCTCGTAGCGAGTTTTTTGCCAAAGGTACGGGCGAGTTTCAGAACGGTCGTCTGATGGTATTGGTTGATGAATTTTCGGCTTCTGCCAGTGAGATTGTGACCGGAGCTGTTCAGGATTGGGACAGAGGCGTTGTGGTAGGCCGTCGCAGTTTTGGTAAAGGCTTGGTGCAGCGTCCCATTGATTTGCCGGACGGTTCGATGATCCGTCTGACGGTGGCCCGCTATTATACGCCTGCCGGACGTTGTATCCAGAAACCTTACGAGAGCATTGAAAAGTATAATGAAGACCTGATAGACCGTTATAATAAAGGAGAAATGATGAGTGAAGACAGCATCCACTTTCCGGATTCACTGAAGTTCAAGACTCATAGGTTGGCCCGTACGGTATATGGTGGCGGAGGGATCATGCCCGATTATTTTGTGCCGATTGATACGACTCTCTATACTAAATACCATCGTCAGTTGCGTGATAAAGGAGCATTGATGAAAGCTCATTTCCATTTTATTGATGCCCATCGTAAAGAATGGCTGGGCAAATACAAGACTTTCAATGAGTTCTACAAACGTTTTGAAGTGACTCCGGATATGTTGACACAACTGGTTGCTACCGGCAAGGAGATGGGAGTGGAATATAATGAAGAAGAGTATCAAAAGGCCCTGCCTCTGCTGAGGTTGCAGATGAAAGCATTGATAGCCCGTGATTTATGGGATATGAATGAATATTATCACGTCATTAATGACGCGAATGAAAGTATCAGGAAAGCGCTGGAACTGTTGGAGCAACCGGACTTTGAGGGGCTGCTGTTGAAAAAGCGATAGTCAAGACTTTATATTAACTGCAGGGGCGCATAGTTGCACTCCTGCAGTTGTCTGAAAAACTTGTAGGATAAAACGAAAGGCTCAGTCGAAAGACTGGGCCTTTCGTTTTACAATTGGGCTACCTTTTTTTTGTTTTGTTTGCAATGGTTAATCTATTGTTAAAATTTAAAGTTTCACTTGAACTTTCAAATAATGTTCTTATATTTGCAGTGTCGAAAGAAACAAAGTAGCCTGGCATCACCTAAAAATTATAAATAAATTAATAAACTTTTATTAAAAAAAAATTACAAAATGAATAATCCACAAACAGGACAATCAACAGGCCTCTTGGGCAATCGTTGGTTCTACTTGGTATTAGCAGTTTTGCTGATGTGTATGATCTCGGGTGTCCAATATTCCTGGACACTGTACGCTAACCCGGTTAAAGACAACCTTGGCGTTTCTTTGGCTGCGGTTCAGACGGCTTTCACACTCTCTCAGGTCATTCAAGCTGGTTCTCAGCCTGGTGGTGGTTACTTCGTTGATAAATTCGGTCCAAGAATTCCATTGATGTTCGGTGGTGCGATGGTTCTCGCTGGCTGGACCTTCATGGGTATGGTTGACAGTGTTCCTGCTCTGTATGCTCTTTATACTCTGGCCGGTGCAGGTGTTGGTATCGTTTACGGTATCGCGATGAACACGGCTAACAGATGGTTCCCGGACAAACGCGGTCTGGCTTCCGGTTTCACCGCTGCCGGTTACGGTCTGGGTGTTCTGCCGTTCCTGCCACTGATCAGCTCCGTTCTGAAAGTTGAAGGTGTTGGCGCAGCATTCATGTACACCGGTTTGATCATGGGTATCCTGATTATCCTGATCGCTTTCGTTATCCGTTTCCCTGGCCAGCAAGGCGCCAAAAAACAAATCGTTGTTACCGACAAGGATTTCAATTCTGGCGAAATGCTGAGAACACCACAATTCTGGGTTCTGTGGACCGCATTCTTTTCCGTTAACTTTGGTGGTTTGCTGCTGGTTGCCAACAGCGTCCCTTACGGTCGCAGCCTCGGTCTTGCCGCAGGTGTGCTGACGATCGGTGTTTCGATCCAGAACCTGTTCAATGGTGGTTGCCGTCCTTTCTGGGGTTTCGTTTCCGATAAAATCGGCCGTTACAAAACCATGTCCGTCGTTTTCGGTATCAATGCTGTTGTTCTCGCACTTTTCCCGACGATTGCTGCCTTGGGCGATGTAGCCTTTATCGCCATGTTGGCAATCGCATTCTTCACATGGGGTGGTAGCTACGCTCTGTTCCCATCGACCAACAGCGATATTTTCGGTACGGCATACTCTGCCAGAAACTATGGTTTCTTCTGGGCTGCAAAAGCAACTGCCTCGATCTTCGGTGGTGGTCTGGGTGCTGCAATTGCAACCAACTTCGGATGGAATACCGCTTTCCTGATTACTGCGATTACTTCTTTCATCGCATTTGCTCTGGCTACCTTCGTTATTCCAAGAATGGGCCGTCCAGTCAAGAAAATGGTCAAATTGTCTCCAGAAGAAAAAGCTGTACATTAAGGTTCCTAACCTGCCTGGCATCTTAATCAATTTTAAACTCACCAAATCAAAATATGAAAAAAAAAAATAATAAAATGTCAACTCCACTTCAAGGAATTAAAGTTCTCGATTTCACCGGTGTGCAATCTGGCCCATCTTGTACTCAAATGCTGGCCTGGTTTGGCGCTGACGTTATTAAAATTGAACGTCCCGGCGTTGGTGACGTAACGCGTCACCAGCTGCGAGATATTCCTGATATCGATGCGCTTTACTTCACCATGCTTAACAGTAACAAACGTTCTATTGAGTTAAATACCAAAACAGCGGAAGGCAAAGAGGTAATGGAAAAGCTGATCCGCGAAGCTGATATCTTAGTCGAGAACTTTCATCCAGGGGCCATTGATCACATGGGCTTCACCTGGGAGCATATTCAAGAAATCAATCCACGTCTGATTTTTGGTTCGATCAAAGGGTTTGATGAGTGTTCGCCTTATGTGAATGTAAAAGCCTATGAAAACGTTGCTCAGGCAGCGGGTGGCGCGGCATCCACTACGGGTTTTTGGGATGGTCCGCCGCTGGTAAGCGCTGCAGCGTTGGGTGACAGCAACACCGGAATGCATTTGCTGATCGGTTTACTTGCTGCTTTGCTGCATCGCGAAAAAACGGGGCGTGGGCAACGAGTCACCATGTCAATGCAGGATGCCGTATTGAACCTTTGCCGCGTGAAATTACGTGACCAGCAGCGTCTAGATAAATTGGGTTATCTGGAAGAATACCCGCAGTATCCGAATGGTACATTTGGTGATGCAGTTCCCCGCGGTGGTAATGCAGGTGGTGGCGGTCAGCCTGGCTGGATCCTGAAATGTAAAGGCTGGGAAACCGATCCTAACGCCTATATTTATTTCACTATTCAGGAGCAAAACTGGGAAAACACCTGTAAAGCCATCGGCAAACCAGAATGGATTACCGATCCGGCATACAGTACAGCCCATGCACGACAGCCACATATTTTCGATATTTTTGCTGAAATCGAAAAATACACTGTCACTATTGATAAACATGAAGCGGTGGCCTATTTGACTCAGTTTGATATTCCTTGTGCACCGGTTTTAAGTATGAAAGAAATTTCACTTGATCCCTCTTTGCGCCAAAGTGGCAGTGTTGTTGAAGTGGAACAACCGTTGCGTGGAAAATATCTGACCGTTGGTTGTCCAATGAAATTCTCTGCCTTTACGCCGGATATTAAAGCTGCGCCGCTATTAGGTGAACATACCGCTGCTGTATTGCAGGAGCTGGGTTATAGCGACGATGAAATTGCTGCAATGAAGCAAAACCACGCCATCTGAGGTTCCTAAACGACCTGGCATCAACTACCTTTATTATTCAACAAACCAATTTAACATATTAAAAAAATAAAAAAATGACTACCACAGCAACGCAGCATTTTGCCCAGTTACTCCAGAAGTACGGAATTGATACTGTCTTCGGCATAGTAGGTATTCCGATCGTGCAGTTGGCCGACACTATGGTCGCGAATGGAATTAAGTTTATACCGTGTCGCAATGAGCAAGCCGCAAGTTACGCGGCAAGCGCCTACGGCTACATCTCGGACAAACCTGGTGTCCTGTTAATCGTGGGAGGGCCTGGCCTTATTCATGCCTTAGCGGGGATCTATAATAGTATGTCGAACCGCTGGCCCTTGTTGGTCATCGCCGGATCCAGCAGCCAGTCAGACATCCACAAGGGAGGTTTCCAGGAACTTGACCAGGTGTCCTTGCTAAGTCCGTTCCTAAAATTCACTGGTAAACTGACGCCGGATAACATCGATATGATAACCCAAAAAGCACTCAACTATTGCATTCAGGGAACCGCCGGAGTGAGCTATATCGATGTCCCCGCTGACTTCATCGAATACGAGAAGCCGCTTGAGGGCAATGATAGAACTGGTAACGAGCTGCCAATGATCCTGACGCCCAATATCTGCGGTCCAGACCCCAGTAAAATTAAGAAGGTGGTGCAATTGATACTCCAGCACAAGAACAAGAACATATTAATAGTGATAGGTAAAGGAGCAGTGAAGAATAGCCATGAGATTAGACGCTTGGTTAACACCTTTAACTTGCCGTTCCTTCCGACTCCCATGGCGAAAGGCATAGTGCCCGATAGCAGCCCTTTAAATGTGAGCAGCGCCCGGAGCCAGGCTCTTAAGATTGCTGACATCGTGTTAGTATTGGGAGCTCGCCTGAATTGGATCCTTCACTTCGGAACGAGTCCAAAGTGGAATAGCGAGAGTATTTTTATACAATTTGACAGTAACCCGGAAACGCTCGGAGACAATAACGTTAGTCCCGGAGCAGATTTAAGTATCTGGGGGGACATCGGACTATCGGTTACTGCACTGGTAGAAGAGCTCACGAGACAGGACAGTTGTTGGAAATACTCGGGAGTCAAACAGGAGATCAGGGAAAAGATACAGTTAAACCAGACCAGATTGCTCCGTAAGGAAAAAACGCGTGGAGCTCAGCTTAACTATAATCAAGTGTACGGCACCCTTCGTCCGTTAATTGACGACTATCGCACGATCTTGGTCACAGAAGGTGCCAACACAATGGACATCGCTAGAATAAGTTTTCCGACTGATGCACCTCGCAGAAGACTTGATGCTGGTACAAATGCTACAATGGGTATAGGTTTAGGTTACGCATTAGCGTGTAAAGCCAGCCACCCTGAGTTAGACGTTGTTTTAATCCAAGGCGACAGTGCCTTCGGTTTTAGCGCTATGGAGATCGAGACTGCCGTCCGTTGCCAATTAGCCCTTGTGATTGTCGTAATGAATAACTCTGGGATATATCACGGTGAGAAAGACATCGAAGGAGATCTGCCCCCGACCGCTTTGTCAAAAAATTGTAGATATGACTTGGTCGGTAAGGGTTTAGGAGCTAACGACTTCTTCGTCAATACCATCTCAGAGTTATCTCGCTGTTTTCAGCAGGCAGTTCAATTGAGCAGAACCAAGCGCGAAACCTCCGTCATTAATGTCATAATCGAACCGGGAGAGCAGAAACAGATCGCGTTCGCATGGCAAAACAAGCCTAGACTGTAAGGTTCCTAATTCCCCTGGCATCAACTACCTAAATTACTTATCAAACCACTTAGTAGTAAAAAAAATATAACAAAATGACAAATAATGAAAGCAAAGGGCCGTTTGAAGGCTTATTAGTTATCGATATGACACATGTCCTTAATGGACCTTTCGGAACTCAACTTCTTTGTAATATGGGCGCAAGGGTAATTAAAGTTGAGCCGCCGGGTCATGGTGATGATACCCGCACATTTGGTCCCTATGTGGATGGACAGTCACTCTATTACAGTTTTATTAATCATGGCAAAGAGAGTGTGGTTCTTGATTTAAAGAATGATCACGATAAAAGTATATTTATAAATATGCTCAAACAAGCTGATGTATTAGCTGAGAATTTTCGCCCAGGTACAATGGAAAAACTGGGGTTTTCATGGGAAACGCTTCAAGAAATCAACCCGCGCCTCATATATGCTTCATCGTCAGGTTTCGGACATACCGGTCCGCTAAAAGATGCTCCTGCCTACGATACCATCATTCAGGCAATGAGCGGGATAATGATGGAAACAGGATATCCTGATGCTCCGCCAGTGCGCGTTGGTACATCTCTTGCGGATCTATGCGGCGGTGTCTATTTATTCAGCGGAATAGTGAGTGCACTTTATGGCCGCGAAAAGAGCCAGAGAGGGGCGCATGTCGATATAGCGATGTTTGATGCCACGCTGAGTTTTCTGGAGCATGGTCTGATGGCATATATCGCAACTGGGAAGTCACCACAACGTCTGGGAAATCGCCATCCCTACATGGCACCTTTTGATGTTTTCAATACTCAGGATAAGCCGATTACGATTTGTTGTGGTAATGACAAGCTTTTTTCTGCGTTATGCCAGGCACTGGAGCTTACGGAACTGGTTAATGATCCCCGATTTAGCAGCAATATTTTACGCGTACAAAACCAGGCTATTCTTAAACAATATATTGAGCGGACGTTAAAAACGCAGGCAGCTGAAGTTTGGTTAGCCAGAATACATGAAGTTGGTGTACCCGTCGCGCCGTTATTAAGTGTGGCTGAGGCCATTAAATTGCCACAAACTCAGGCGAGAAATATGTTGATTGAAGCCGGGGGAATAATGATGCCGGGTAATCCGATAAAAATCAGCGGCTGCGCGGACCCGCATGTTATGCCGGGAGCGGCAACGCTCGACCAGCATGGGGAACAAATTCGCCAGGAGTTCTCATCATAAGGTTCCTAAAATGCCTGGCATCTTTTCCATAACAAAAATTAAACCTCCTCCATATGAAAAATAAAAATTATAAAATGACAAGTGCCGCTACTGTTACTGCTTCGTTCAACGATACTTTTAGCGTATCCGATAATGTCGCCGTTATTGTTCCTGAAACGGACACTCAGGTGACCTACAGGGATCTATCCCACATGGTGGGTCACTTCCAGACCATGTTCACAAATCCTAATTCTCCATTGTACGGAGCTGTTTTCAGACAAGATACAGTGGCGATATCCATGCGTAATGGGCTGGAATTTATCGTCGCTTTCCTCGGTGCTACTATGGACGCTAAAATTGGCGCGCCCTTGAATCCCAATTATAAGGAAAAGGAGTTCAATTTTTATTTGAATGACCTGAAATCTAAGGCGATTTGCGTCCCAAAGGGTACCACAAAGTTACAGAGTTCTGAAATTCTAAAATCTGCCTCCACGTTTGGATGTTTTATCGTAGAGCTGGCCTTCGATGCGACCAGGTTTAGGGTAGAGTATGATATATACTCTCCAGAGGACAACTACAAAAGGGTTATTTACCGGTCTTTGAACAACGCCAAATTTGTCAACACAAATCCCGTTAAATTCCCTGGGTTTGCCCGTTCCAGTGACGTTGCCCTGATTTTGCATACCAGTGGTACCACCTCCACTCCAAAAACGGTGCCTTTGTTACATTTGAACATTGTGAGAAGCACGTTGAACATTGCTAACACTTACAAGCTAACGCCCTTGGACAGATCTTATGTCGTGATGCCTCTTTTCCACGTCCATGGGTTAATTGGTGTTTTACTTTCCACTTTTAGAACTCAGGGTTCTGTTGTGGTTCCCGATGGATTCCATCCAAAGTTATTCTGGGACCAATTTGTTAAGTACAACTGTAATTGGTTCAGTTGCGTTCCCACAATAAGCATGATTATGCTGAACATGCCCAAACCAAACCCTTTCCCACACATTAGATTCATCAGATCGTGTTCTTCTGCTTTGGCTCCAGCAACGTTCCATAAGCTGGAGAAGGAATTCAATGCACCTGTCTTGGAGGCCTATGCGATGACCGAAGCATCACATCAAATGACCTCAAACAATCTGCCTCCAGGAAAGAGAAAGCCTGGTACTGTGGGCCAGCCACAAGGAGTCACCGTCGTCATTCTAGATGACAATGACAATGTCTTGCCCCCGGGCAAAGTCGGCGAAGTTTCCATCAGAGGCGAAAACGTCACTTTGGGGTATGCTAATAATCCAAAAGCTAACAAGGAGAACTTCACCAAGAGAGAGAACTATTTCAGAACCGGTGACCAAGGTTATTTCGACCCTGAGGGGTTTTTGGTCCTTACAGGCAGAATCAAAGAGCTTATCAACAGGGGTGGTGAAAAGATTTCACCCATTGAGCTCGACGGCATTATGCTATCGCATCCAAAGATCGATGAAGCCGTTGCATTTGGTGTTCCCGACGATATGTACGGCCAAGTAGTTCAAGCCGCCATTGTTTTGAAGAAGGGAGAAAAAATGACCTACGAAGAACTGGTGAACTTCTTAAAGAAGCACCTAGCCTCTTTCAAAATTCCAACCAAGGTGTACTTTGTTGATAAGCTACCAAAAACCGCTACAGGTAAAATCCAGAGAAGAGTTATCGCAGAAACTTTTGCTAAGAGCAGCAGAAATAAGAGTAAGTTGTAGGGTTCCTAAAAACCCTGGCATCTAAACTAAAATACAATAACCAAAAATCATTACAAATGAAAAAAAATAATAAAATGGATAGCGATACTCTCTCAGGATTATTGGAAAACGTCGCCAAAAAATTCCCCGATCGCCGAGCTCTCTCCGTTTCTGGAAAATTCAATCTCACTCACGCGCGTCTTCACGATCTAATCGAACGCGCCGCTTCACGCCTTGTCTCCGACGCTGGAATCAAACCCGGCGATGTCGTTGCTCTCACCTTCCCTAACACCGTCGAGTTTGTTATAATGTTTTTAGCGGTGATAAGAGCTAGAGCCACGGCGGCGCCGTTGAACGCAGCGTACACGGCGGAGGAATTTGAGTTTTACCTCTCCGATTCAGATTCAAAGCTATTGTTAACCTCTAAAGAAGGAAACGCACCGGCTCAAGAAGCAGCTTCAAAGCTGAAAATCTCTCACGTCACCGCTACGCTGCTTGACGCTGGCTCGGACCTTGTACTATCCGTTGCGGATTCAGATTCCGTCGTTGACTCAGCGACGGAACTCGTTAATCACCCGGACGACGGTGCTCTCTTCCTCCACACTTCTGGCACTACGAGCCGTCCAAAGGGTGTACCGCTTACGCAGCTCAATCTAGCTTCATCCGTCAAGAACATTAAAGCTGTGTACAAGCTTACTGAGTCTGATTCTACGGTGATTGTTCTCCCTCTGTTCCATGTTCATGGATTGTTAGCTGGGTTGCTTAGCTCGCTTGGAGCTGGTGCTGCTGTAACTCTTCCAGCTGCTGGTAGATTCTCAGCAACAACATTTTGGCCAGATATGAAGAAGTATAACGCTACATGGTATACTGCTGTGCCGACCATTCATCAGATCATATTGGACCGCCACGCGAGCCACCCTGAAACGGAATATCCTAAACTCCGGTTCATCAGGAGTTGCAGTGCTTCTTTGGCTCCGGTGATATTGTCCAGGCTTGAGGAAGCGTTTGGAGCACCGGTGCTCGAGGCCTATGCAATGACAGAGGCAACACATTTGATGAGCTCAAACCCCTTACCAGAGGAAGGTCCACACAAGCCTGGGTCTGTTGGGAAACCGGTAGGTCAAGAAATGGCGATCCTTAATGAGAAAGGCGAGATCCAAGAGCCAAATAACAAAGGAGAGGTTTGTATAAGAGGTCCAAATGTGACCAAGGGTTACAAGAATAACCCAGAAGCCAACAAGGCAGGTTTCGAGTTTGGGTGGTTCCACACTGGTGATATCGGTTACTTTGATACCGATGGGTATTTGCATCTGGTGGGTCGGATCAAAGAGCTTATTAACCGTGGAGGTGAGAAGATATCTCCAATTGAAGTGGATGCAGTACTCTTAACGCATCCTGACGTTTCTCAGGGTGTTGCATTCGGTGTTCCTGATGAGAAATATGGGGAAGAGATTAACTGTGCGGTGATTCCAAGAGAAGGAACTACTGTAACCGAAGAGGACATTAAAGCGTTTTGTAAGAAGAATTTGGCAGCTTTCAAGGTGCCAAAGAGAGTGTTCATCACTGATAACCTCCCCAAAACTGCCTCTGGTAAGATTCAGCGCCGTATCGTCGCACAACATTTCCTTGAGAAGCCCTGAGGTTCCTAGCTGATTAGAAGGCCATCCTGACGGATGGCCTTTTTTTTGACTCTGTTGTGTCTTCTTTTAGGTGTGGAATAATTTTCCAACCATCACTTTATCTGTCTCCCGAACAGCACCTTATTCAGCCATCGGTTCACATACACGTTCACGAAAGCACATCCTACTCCGATAGCGGCTCCTGCCACTACATCACTGGGATAGTGCACCCCTTGGTTCATCCGGGCAAACCCTACTCCGCAAGCCCATACGGCTGAAGGGGCGATAACATACCACTTAGGATAAGTGATGGAGAGTGAGGTGGCTAAAGAAAAAGCTGCCGCAGTGTGTCCTGAAGGGAATGACGGACTATCGGCATCCGGTGCTCCGTATGCATGAATCTTATCCGGATACTTTACAAAAGGCCGGTCACGACCTATTATATGTTTGGCGGCGTATGTAATCCCCACTGCTTCAATCACACTGGTTCCTATGTAAATGGCATCTTTCAGCATCGGCTGGTCTTTCTTTATCAATGCATAGAGTCCCATGGCTGTAGGTATTCCTACGGGTAGAATCACTCCGCTAGCGGACAATCCGCGGCTCAGTCCGTGTACATCCCATCGGTTGATGGTTTTCAACGTGTTGATGTCTATATTCTGTGCGCTGAGGCTGAATGTAAAACAGGCTGTCAGTATAAGAAAAATAATCCTTTGGCGTTTCATTTAGTTTGGCATTTGTTCAGTGCAAAGATAGGAAAATAATGTAATTCGCATTTACCCTTCCCCGAATTAAAGAATAATTTCCATAATTCCTTGTATTATCGCTAGAATTCCTTTACTTTTGCATCGTGAATACACAGCTTTTAACAGAATTTTTAGTTCTAACAATTAAATAATTTAAATTCAATCATTTATGTGGTTAAGTAATTCATCTATTGGAAGGAAAGTGGTGATGAGTGTCACAGGTATCGCCCTTGTCCTGTTTCTGACGTTTCACATGGCGATGAACCTTGTTGCGCTCTTCTCGGAAGATGCTTACAACATGATTTGTGAATTCTTGGGAGCTAACTGGTACGCGTTGGTTGCAACTGCCGGGCTGGCTGCTCTGTTTGTAATTCACATCATCTACGCTTTCTGGTTGACCATGCAGAACCGTGCAGCTCGCGGTAGCGAACGTTATGCAGTGAACGTTAAACCTAAAACTGTGGAATGGGCATCTCAGAACATGCTTGTCCTGGGTATTGTCGTTATTGCCGGATTGGCTCTTCATTTTGCCAATTTCTGGTACAAAATGCAGTTTGCCGAAATTATCGGTGTTCATGATTTGGGCGCATTCGGTCCTACAGACGGTGCCGCTTACATTCGTGAAACATTCGGATGCCCTGTATTTACAGTGCTTTACCTCATTTGGTTAGCAGCTTTGTGGTTCCACCTGACTCACGGATTCTGGAGTGCTTTGCAGACTTTGGGCTGGAGCAATAAAATCTGGTTCGAGCGTTGGAAGACTATTGGTAACATCTACTCTACTTTGGTAGTTCTTGGTTTTGCAGCAGTAGTCGTTATCTACTTCGCTATGACTGGCGCGCCTGATAGGTGGGCTGCCCTTCCTGGTTGGCTTGGTTTCATCAGCCATCCGCTTGCCCTCATCTGTTACGCCGGCGGTAGCCGGCCAGCCTCGCAGAGCAGGATTCCCGTTGAGCACCGCCAGGTGCGAATAAGGGACAGTGAAGAAGGAACACCCGCTCGCGGGTGGGCCTACTTCACCTATCCTGCCCGGCTGACGCCGTTGGATACACCAAGGAAAGTCTACACGAACCCTTTGGCAAAATCCTGTATATCGTGCGAAAAAGGATGGATATACCGAAAAAATCGCTATAATGACCCCGAAGCAGGGTTATGCAGCGGAAAAGCGGGATTAAAAGTCGGGGATTGGTGAACAAAAAGGTGTTTCTCTCTTTAAGAGAAATATCGTTTTGCTAAACAGTTGATATTGAGGTATCATTTTATCGTAAAAGACATTTTTGCTCAACAATTGCTTGACGGAAATCAACAAATTTTAGCATTTTGTAAAAAAGTCGCTATATAATTTGGTGAATTGGAGTTATTTTCATATTTTTGCATCCCGAAGAGTTTCTCTTAAAGAGAGAAACATCTTTTGCATACCTTTTCCGACCGAATTTTTATGTCGTAAAGAGGGGCTTTGCAGGGGGTGGACTCAGAAAGATGAGAATAGATGACTATTGTAGTTGAAACACATAGAAAGTTGCTGATATACAGACCGATACGCATATCGGGATGAACCATGAGTACGTTCTTTTCTCAAAAAACATAAATATTCGAAAAGAGATGCAATAAATTAAGGAGAGGTTATAATGAACAAAGTAAATATAAAAGATAGTCAAAATTTTATTACTTCAAAATATCACATAGAAAAAATAATGAATTGCATAAGTTTAGATGAAAAAGATAACATCTTTGAAATAGGTGCAGGGAAAGGTCATTTTACTGCTGGATTGGTAAAGAGATGTAATTTTGTAACGGCGATAGAAATTGATTCTAAATTATGTGAGGTAACTCGTAATAAGCTCTTAAATTATCCTAACTATCAAATAGTAAATGATGATATACTGAAATTTACATTTCCTAGCCACAATCCATATAAAATATTTGGCAGCATACCTTACAACATAAGCACAAATATAATTCGAAAAATTGTTTTTGAAAGTTCAGCCACAATAAGTTATTTAATAGTGGAATATGGTTTTGCTAAAATGTTATTAGATACAAACAGATCACTAGCATTGCTGTTAATGGCAGAGGTAGATATTTCTATATTAGCAAAAATTCCTAGGTATTATTTCCATCCAAAACCTAAAGTGGATAGCACATTAATTGTATTAAAAAGAAAGCCAGCAAAAATGGCATTTAAAGAGAGAAAAAAATATGAAACTTTTGTAATGAAATGGGTTAACAAAGAGTACGAAAAACTGTTTACAAAAAATCAATTTAATAAAGCTTTAAAACATGCGAGAATATATGATATAAACAATATTAGTTTCGAACAATTTGTATCGCTATTTAATAGTTATAAAATATTTAACGGCTAAAAACAATAGGCCACATGCAACTGTAAATGTTTACGCGGGTACCGACACCGCGGTGGAGGGGAATTGTGTTACAACCAATTAACCAATTCTGATTAGAAAAACTCATCGAGCATCAAATGAAACTGCAATTTATTCATATCAGGATTATCAATACCATATTTTTGAAAAAGCCGTTTCTGTAATGAAGGAGAAAACTCACCGAGGCAGTTCCATAGGATGGCAAGATCCTGGTATCGGTCTGCGATTCCGACTCGTCCAACATCAATACAACCTATTAATTTCCCCTCGTCAAAAATAAGGTTATCAAGTGAGAAATCACCATGAGTGACGACTGAATCCGGTGAGAATGGCAAAAGCTTATGCATTTCTTTCCAGACTTGTTCAACAGGCCAGCCATTACGCTCGTCATCAAAATCACTCGCATCAACCAAACCGTTATTCATTCGTGATTGCGCCTGAGCGAGGCGAAATACGCGATCGCTGTTAAAAGGACAATTACAAACAGGAATCGAATGCAACCGGCGCAGGAACACTGCCAGCGCATCAACAATATTTTCACCTGAATCAGGATATTCTTCTAATACCTGGAATGCTGTTTTCCCGGGGATCGCAGTGGTGAGTAACCATGCATCATCAGGAGTACGGATAAAATGCTTGATGGTCGGAAGAGGCATAAATTCCGTCAGCCAGTTTAGTCTGACCATCTCATCTGTAACATCATTGGCAACGCTACCTTTGCCATGTTTCAGAAACAACTCTGGCGCATCGGGCTTCCCATACAATCGATAGATTGTCGCACCTGATTGCCCGACATTATCGCGAGCCCATTTATACCCATATAAATCAGCATCCATGTTGGAATTTAATCGCGGCCTGGAGCAAGACGTTTCCCGTTGAATATGGCTCATAACACCCCTTGTATTACTGTTTATGTAAGCAGACAGTTTTATTGTTCATGATGATATATTTTTATCTTGTGCAATGTAACATCAGAGATTTTGAGACACAACGTGGCTTTGTTGAATAAATCGAACTTTTGCTGAGTTGAAGGATCAGAGCCTACGTTCCGAATACGGTCAAAAAAAAGGCCATCCGTCAGGATGGCCTTCGCATTAATATGCCGCTTCGAATTCTTTTAGGAAGCGTGTATCGTTTTCAGAGAACATACGGAGGTCTTTCACCTGATATTTCAGGTTTGTGATACGCTCGATACCCATACCGAGTCCATAACCGCTGTATATTTTGCTGTCTATACCATTTGATTCAAGTACGTTCGGGTCTACCATACCGCAACCGAGGATTTCTACCCAGCCGGTGTGTTTACAGAACGGACATCCTTTACCGCCGCAGATATTACAGCTGATATCCATTTCCGCACTTGGTTCAGCAAACGGGAAGTAAGACGGACGCAGACGGATCTTTGTATCAGCACCGAACATTTCTTTGGCAAAGAGCAGCAATACCTGCTTCAAGTCGGTGAATGATACGTTTTTATCTACATACAGCGCTTCTACCTGATGGAAGAAACAGTGTGCGCGATAGCTGATAGCTTCGTTACGATATACACGTCCCGGACAGATGATGCGGATAGGAGGCTGTGAAGTTTCCATCACACGAGTCTGTACAGAAGAAGTATGTGTACGCAATACTACGTCCGGGTGAGCTTCGATAAAGAAAGTGTCCTGCATATCGCGTGCCGGATGATCTTCGGCAAAGTTCAGTGCCGAGAACACGTGCCAGTCATCTTCAATTTCCGGACCTTCGGCAATGCTGAATCCCAGACGGGCAAAGATATCAATGATTTCGTTCTTTACAATGGTGAGCGGGTGGCGTGTACCGAGTTCTACAGGATAAGCCGAACGCGTCAAATCCAGTCCGTCACAATCGTTGTCCTGACTTTCAAACATTTCTTTCAGCGCGTTGATTTTGTCCTGCGCTTTTGTTTTCAGTTCATTCAGTCTCATGCCGACTTCTTTTTTCTGTTCGGCAGCTACATTACGGAAATCTGCCATTAAGTCGTTAATGGCTCCCTTCTTACTTAGGTATTTGATGCGGAGAGCTTCGAGTTCTTCGGCATTGGAGGCGTGTAAGGCTTCCACCTCTTTCAGAAGTTGTTCAATCTTAGCTATCATTTTTTAATATTTTTAGCGGCCCCGTTAAACAAAATTATTTGTAGAGGCTGTTTCGTCCTCACGGACTCATCAGACCGGAAAGCACATCCGGTGACAGCTCAGGCTACTTTGTTTCTTTCGACACTGCAAATATAAGAACATTATTTGAAAGTTCAAGTGAAACTTTAAATTTTAACAATAGATTAACCATTGCAAACAAAACAAAAAAAAGGTAGCCCAATTGTAAAACGAAAGGCCCAGTCTTTCGACTGAGCCTTTCGTTTTATCCTACAGTCGCTCGGCGATCGAAGGCTTCGGAAAAAAAAGGCCATCCGTCAGGATGGCCTTCGCATTAATATGCCGCTTCGAATTCTTTTAGGAAGCGTGTATCGTTTTCAGAGAACATACGGAGGTCTTTCACCTGATATTTCAGGTTTGTGATACGCTCGATACCCATACCGAGTCCATAACCGCTGTATATTTTGCTGTCTATACCATTTGATTCAAGTACGTTCGGGTCTACCATACCGCAACCGAGGATTTCTACCCAGCCGGTGTGTTTACAGAACGGACATCCTTTACCGCCGCAGATATTACAGCTGATATCCATTTCCGCACTTGGTTCAGCAAACGGGAAGTAAGACGGACGCAGACGGATCTTTGTATCAGCACCGAACATTTCTTTGGCAAAGAGCAGCAATACCTGCTTCAAGTCGGTGAATGATACGTTTTTATCTACATACAGCGCTTCTACCTGATGGAAGAAACAGTGTGCGCGATAGCTGATAGCTTCGTTACGATATACACGTCCCGGACAGATGATGCGGATAGGAGGCTGTGAAGTTTCCATCACACGAGTCTGTACAGAAGAAGTATGTGTACGCAATACTACGTCCGGGTGAGCTTCGATAAAGAAAGTGTCCTGCATATCGCGTGCCGGATGATCTTCGGCAAAGTTCAGTGCCGAGAACACGTGCCAGTCATCTTCAATTTCCGGACCTTCGGCAATGCTGAATCCCAGACGGGCAAAGATATCAATGATTTCGTTCTTTACAATGGTGAGCGGGTGGCGTGTACCGAGTTCTACAGGATAAGCCGAACGCGTCAAATCCAGTCCGTCACAATCGTTGTCCTGACTTTCAAACATTTCTTTCAGCGCGTTGATTTTGTCCTGCGCTTTTGTTTTCAGTTCATTCAGTCTCATGCCGACTTCTTTTTTCTGTTCGGCAGCTACATTACGGAAATCTGCCATTAAGTCGTTAATGGCTCCCTTCTTACTTAGGTATTTGATGCGGAGAGCTTCGAGTTCTTCGGCATTGGAGGCGTGTAAGGCTTCCACCTCTTTCAGAAGTTGTTCAATCTTAGCTATCATTTTTTAATATTTTTAGCGGCCCCGTTAAACAAAATTATTTGTAGAGGCTGTTTCGTCCTCACGGACTCATCAGACCGGAAAGCACATCCGGTGACAGCTCAGGCTACTTTGTTTCTTTCGACACTGCAAATATAAGAACATTATTTGAAAGTTCAAGTGAAACTTTAAATTTTAACAATAGATTAACCATTGCAAACAAAACAAAAAAAAGGTAGCCCAATTGTAAAACGAAAGGCCCAGTCTTTCGACTGAGCCTTTCGTTTTATCCTAGGATCAGCTGTACGTACTCGCAGTTCAACCTGTTGATAGTACGTACTAAGCTCTCATGTTTCACGTACTAAGCTCTCATGTTTAACGTACTAAGCTCTCATGTTTAACGAACTAAACCCTCATGGCTAACGTACTAAGCTCTCATGGCTAACGTACTAAGCTCTCATGTTTCACGTACTAAGCTCTCATGTTTGAACAATAAAATTAATATAAATCAGCAACTTAAATAGCCTCTAAGGTTTTAAGTTTTATAAGAAAAAAAAGAATATATAAGGCTTTTAAAGCTTTTAAGGTTTAACGGTTGTGGACAACAAGCCAGGGATGTAACGCACTGAGAAGCCCTTAGAGCCTCTCAAAGCAATTTTGAGTGACACAGGAACACTTAACGGCTGACATGGGGCGGCCGCGTAG
SEQ ID NO:95(核糖体结合位点和At_AAE3开放读码框):
GGAGAGCTGTATGTGGGGTGGAAGAAGCTATCGTAAAAATGCTGGAGCAGCTTGATCCTCATTCTACCTATTCTGATCCGGAGGAAGTGAAGAGAATGAACGAGCCGTTGCAGGGAAACTTCGAAGGTATCGGTATACAGTTTAATATGGCGGAGGACACTTTGCTGGTGATACAGCCTGTATCGGGCGGACCGTCCGAAAAAGTCGGTATTCTGGCCGGTGATCGTATCGTCATGGTGGAAGATACGCTGATAGCCGGTGTAAAGATGAGTACCGAGGATATTATGCGTCGCCTCCGTGGTCCTAAAGATTCAAAAGTGAATCTGAAAATACTTCGCCGAGGGGTGAAGGAACTGCTCCCCTTTACGGTGAAAAGGGATAAAATACCTGTTTACAGCTTGGATGCTTCCTATATGATTAAAGATAAGATCGGTTATATTCGTATCAACCGTTTTGCCGCCACTACCCATGAGGAGTTCAAGAAAGCGCTGACCGGGTTGCAGAAGAAAGGAATGAAAGACTTGATTCTTGATTTGCAGGGCAATGGCGGGGGGTATTTGAATGCTGCTATCGATATTGCCAATGAATTTTTGGGTAAAAAGGAACTGATTGTCTATACGGAGGGCCGTCGTAATCCTCGTAGCGAGTTTTTTGCCAAAGGTACGGGCGAGTTTCAGAACGGTCGTCTGATGGTATTGGTTGATGAATTTTCGGCTTCTGCCAGTGAGATTGTGACCGGAGCTGTTCAGGATTGGGACAGAGGCGTTGTGGTAGGCCGTCGCAGTTTTGGTAAAGGCTTGGTGCAGCGTCCCATTGATTTGCCGGACGGTTCGATGATCCGTCTGACGGTGGCCCGCTATTATACGCCTGCCGGACGTTGTATCCAGAAACCTTACGAGAGCATTGAAAAGTATAATGAAGACCTGATAGACCGTTATAATAAAGGAGAAATGATGAGTGAAGACAGCATCCACTTTCCGGATTCACTGAAGTTCAAGACTCATAGGTTGGCCCGTACGGTATATGGTGGCGGAGGGATCATGCCCGATTATTTTGTGCCGATTGATACGACTCTCTATACTAAATACCATCGTCAGTTGCGTGATAAAGGAGCATTGATGAAAGCTCATTTCCATTTTATTGATGCCCATCGTAAAGAATGGCTGGGCAAATACAAGACTTTCAATGAGTTCTACAAACGTTTTGAAGTGACTCCGGATATGTTGACACAACTGGTTGCTACCGGCAAGGAGATGGGAGTGGAATATAATGAAGAAGAGTATCAAAAGGCCCTGCCTCTGCTGAGGTTGCAGATGAAAGCATTGATAGCCCGTGATTTATGGGATATGAATGAATATTATCACGTCATTAATGACGCGAATGAAAGTATCAGGAAAGCGCTGGAACTGTTGGAGCAACCGGACTTTGAGGGGCTGCTGTTGAAAAAGCGATAGTCAAGACTTTATATTAACTGCAGGGGCGCATAGTTGCACTCCTGCAGTTGTCTGAAAAACTTGTAGGATAAAACGAAAGGCTCAGTCGAAAGACTGGGCCTTTCGTTTTACAATTGGGCTACCTTTTTTTTGTTTTGTTTGCAATGGTTAATCTATTGTTAAAATTTAAAGTTTCACTTGAACTTTCAAATAATGTTCTTATATTTGCAGTGTCGAAAGAAACAAAGTAGCCTGGCATCATCTACCTTCATTATTAATCATTCCAATTGTATCACTTAAAAATATAAAAAAATGTCAACTCCACTTCAAGGAATTAAAGTTCTCGATTTCACCGGTGTGCAATCTGGCCCATCTTGTACTCAAATGCTGGCCTGGTTTGGCGCTGACGTTATTAAAATTGAACGTCCCGGCGTTGGTGACGTAACGCGTCACCAGCTGCGAGATATTCCTGATATCGATGCGCTTTACTTCACCATGCTTAACAGTAACAAACGTTCTATTGAGTTAAATACCAAAACAGCGGAAGGCAAAGAGGTAATGGAAAAGCTGATCCGCGAAGCTGATATCTTAGTCGAGAACTTTCATCCAGGGGCCATTGATCACATGGGCTTCACCTGGGAGCATATTCAAGAAATCAATCCACGTCTGATTTTTGGTTCGATCAAAGGGTTTGATGAGTGTTCGCCTTATGTGAATGTAAAAGCCTATGAAAACGTTGCTCAGGCAGCGGGTGGCGCGGCATCCACTACGGGTTTTTGGGATGGTCCGCCGCTGGTAAGCGCTGCAGCGTTGGGTGACAGCAACACCGGAATGCATTTGCTGATCGGTTTACTTGCTGCTTTGCTGCATCGCGAAAAAACGGGGCGTGGGCAACGAGTCACCATGTCAATGCAGGATGCCGTATTGAACCTTTGCCGCGTGAAATTACGTGACCAGCAGCGTCTAGATAAATTGGGTTATCTGGAAGAATACCCGCAGTATCCGAATGGTACATTTGGTGATGCAGTTCCCCGCGGTGGTAATGCAGGTGGTGGCGGTCAGCCTGGCTGGATCCTGAAATGTAAAGGCTGGGAAACCGATCCTAACGCCTATATTTATTTCACTATTCAGGAGCAAAACTGGGAAAACACCTGTAAAGCCATCGGCAAACCAGAATGGATTACCGATCCGGCATACAGTACAGCCCATGCACGACAGCCACATATTTTCGATATTTTTGCTGAAATCGAAAAATACACTGTCACTATTGATAAACATGAAGCGGTGGCCTATTTGACTCAGTTTGATATTCCTTGTGCACCGGTTTTAAGTATGAAAGAAATTTCACTTGATCCCTCTTTGCGCCAAAGTGGCAGTGTTGTTGAAGTGGAACAACCGTTGCGTGGAAAATATCTGACCGTTGGTTGTCCAATGAAATTCTCTGCCTTTACGCCGGATATTAAAGCTGCGCCGCTATTAGGTGAACATACCGCTGCTGTATTGCAGGAGCTGGGTTATAGCGACGATGAAATTGCTGCAATGAAGCAAAACCACGCCATCTGAGGTTCCTAACCTGCCTGGCATCAACTACCTAAATTACTTATCAAACCACTTAGTAGTAAAAAAAATATAACAAAATGACAAATAATGAAAGCAAAGGGCCGTTTGAAGGCTTATTAGTTATCGATATGACACATGTCCTTAATGGACCTTTCGGAACTCAACTTCTTTGTAATATGGGCGCAAGGGTAATTAAAGTTGAGCCGCCGGGTCATGGTGATGATACCCGCACATTTGGTCCCTATGTGGATGGACAGTCACTCTATTACAGTTTTATTAATCATGGCAAAGAGAGTGTGGTTCTTGATTTAAAGAATGATCACGATAAAAGTATATTTATAAATATGCTCAAACAAGCTGATGTATTAGCTGAGAATTTTCGCCCAGGTACAATGGAAAAACTGGGGTTTTCATGGGAAACGCTTCAAGAAATCAACCCGCGCCTCATATATGCTTCATCGTCAGGTTTCGGACATACCGGTCCGCTAAAAGATGCTCCTGCCTACGATACCATCATTCAGGCAATGAGCGGGATAATGATGGAAACAGGATATCCTGATGCTCCGCCAGTGCGCGTTGGTACATCTCTTGCGGATCTATGCGGCGGTGTCTATTTATTCAGCGGAATAGTGAGTGCACTTTATGGCCGCGAAAAGAGCCAGAGAGGGGCGCATGTCGATATAGCGATGTTTGATGCCACGCTGAGTTTTCTGGAGCATGGTCTGATGGCATATATCGCAACTGGGAAGTCACCACAACGTCTGGGAAATCGCCATCCCTACATGGCACCTTTTGATGTTTTCAATACTCAGGATAAGCCGATTACGATTTGTTGTGGTAATGACAAGCTTTTTTCTGCGTTATGCCAGGCACTGGAGCTTACGGAACTGGTTAATGATCCCCGATTTAGCAGCAATATTTTACGCGTACAAAACCAGGCTATTCTTAAACAATATATTGAGCGGACGTTAAAAACGCAGGCAGCTGAAGTTTGGTTAGCCAGAATACATGAAGTTGGTGTACCCGTCGCGCCGTTATTAAGTGTGGCTGAGGCCATTAAATTGCCACAAACTCAGGCGAGAAATATGTTGATTGAAGCCGGGGGAATAATGATGCCGGGTAATCCGATAAAAATCAGCGGCTGCGCGGACCCGCATGTTATGCCGGGAGCGGCAACGCTCGACCAGCATGGGGAACAAATTCGCCAGGAGTTCTCATCATAAGGTTCCTAAACGACCTGGCATCATCTACCTTCATTATTAAACAAACCAAATTAATATCATAAAAAAATAAAAAAATGACTACCACAGCAACGCAGCATTTTGCCCAGTTACTCCAGAAGTACGGAATTGATACTGTCTTCGGCATAGTAGGTATTCCGATCGTGCAGTTGGCCGACACTATGGTCGCGAATGGAATTAAGTTTATACCGTGTCGCAATGAGCAAGCCGCAAGTTACGCGGCAAGCGCCTACGGCTACATCTCGGACAAACCTGGTGTCCTGTTAATCGTGGGAGGGCCTGGCCTTATTCATGCCTTAGCGGGGATCTATAATAGTATGTCGAACCGCTGGCCCTTGTTGGTCATCGCCGGATCCAGCAGCCAGTCAGACATCCACAAGGGAGGTTTCCAGGAACTTGACCAGGTGTCCTTGCTAAGTCCGTTCCTAAAATTCACTGGTAAACTGACGCCGGATAACATCGATATGATAACCCAAAAAGCACTCAACTATTGCATTCAGGGAACCGCCGGAGTGAGCTATATCGATGTCCCCGCTGACTTCATCGAATACGAGAAGCCGCTTGAGGGCAATGATAGAACTGGTAACGAGCTGCCAATGATCCTGACGCCCAATATCTGCGGTCCAGACCCCAGTAAAATTAAGAAGGTGGTGCAATTGATACTCCAGCACAAGAACAAGAACATATTAATAGTGATAGGTAAAGGAGCAGTGAAGAATAGCCATGAGATTAGACGCTTGGTTAACACCTTTAACTTGCCGTTCCTTCCGACTCCCATGGCGAAAGGCATAGTGCCCGATAGCAGCCCTTTAAATGTGAGCAGCGCCCGGAGCCAGGCTCTTAAGATTGCTGACATCGTGTTAGTATTGGGAGCTCGCCTGAATTGGATCCTTCACTTCGGAACGAGTCCAAAGTGGAATAGCGAGAGTATTTTTATACAATTTGACAGTAACCCGGAAACGCTCGGAGACAATAACGTTAGTCCCGGAGCAGATTTAAGTATCTGGGGGGACATCGGACTATCGGTTACTGCACTGGTAGAAGAGCTCACGAGACAGGACAGTTGTTGGAAATACTCGGGAGTCAAACAGGAGATCAGGGAAAAGATACAGTTAAACCAGACCAGATTGCTCCGTAAGGAAAAAACGCGTGGAGCTCAGCTTAACTATAATCAAGTGTACGGCACCCTTCGTCCGTTAATTGACGACTATCGCACGATCTTGGTCACAGAAGGTGCCAACACAATGGACATCGCTAGAATAAGTTTTCCGACTGATGCACCTCGCAGAAGACTTGATGCTGGTACAAATGCTACAATGGGTATAGGTTTAGGTTACGCATTAGCGTGTAAAGCCAGCCACCCTGAGTTAGACGTTGTTTTAATCCAAGGCGACAGTGCCTTCGGTTTTAGCGCTATGGAGATCGAGACTGCCGTCCGTTGCCAATTAGCCCTTGTGATTGTCGTAATGAATAACTCTGGGATATATCACGGTGAGAAAGACATCGAAGGAGATCTGCCCCCGACCGCTTTGTCAAAAAATTGTAGATATGACTTGGTCGGTAAGGGTTTAGGAGCTAACGACTTCTTCGTCAATACCATCTCAGAGTTATCTCGCTGTTTTCAGCAGGCAGTTCAATTGAGCAGAACCAAGCGCGAAACCTCCGTCATTAATGTCATAATCGAACCGGGAGAGCAGAAACAGATCGCGTTCGCATGGCAAAACAAGCCTAGACTGTAAGGTTCCTAAGGGTCCTGGCATCCCCAAAAAATTAATTCATTTTCTCTTTTCCCTAACCCTTAAAATATAATAAAATGAGTAACGACGACAATGTAGAGTTGACTGATGGCTTTCATGTTTTGATCGATGCCCTGAAAATGAATGACATCGATACCATGTATGGTGTTGTCGGCATTCCTATCACGAACCTGGCTCGTATGTGGCAAGATGACGGTCAGCGTTTTTACAGCTTCCGTCACGAACAACACGCAGGTTATGCAGCTTCTATCGCCGGTTACATCGAAGGAAAACCTGGCGTTTGCTTGACCGTTTCCGCCCCTGGCTTCCTGAACGGCGTGACTTCCCTGGCTCATGCAACCACCAACTGCTTCCCAATGATCCTGTTGAGCGGTTCCAGTGAACGTGAAATCGTCGATTTGCAACAGGGCGATTACGAAGAAATGGATCAGATGAATGTTGCACGTCCACACTGCAAAGCTTCTTTCCGTATCAACAGCATCAAAGACATTCCAATCGGTATCGCTCGTGCAGTTCGCACCGCTGTATCCGGACGTCCAGGTGGTGTTTACGTTGACTTGCCAGCAAAACTGTTCGGTCAGACCATTTCTGTAGAAGAAGCTAACAAACTGCTCTTCAAACCAATCGATCCAGCTCCGGCACAGATTCCTGCTGAAGATGCTATCGCTCGCGCTGCTGACCTGATCAAGAACGCCAAACGTCCAGTTATCATGCTGGGTAAAGGCGCTGCATACGCACAATGCGACGACGAAATCCGCGCACTGGTTGAAGAAACCGGCATCCCATTCCTGCCAATGGGTATGGCTAAAGGCCTGCTGCCTGACAACCATCCACAATCCGCTGCTGCAACCCGTGCTTTCGCACTGGCACAGTGTGACGTTTGCGTACTGATCGGCGCTCGTCTGAACTGGCTGATGCAGCACGGTAAAGGCAAAACCTGGGGCGACGAACTGAAGAAATACGTTCAGATCGACATCCAGGCTAACGAAATGGACAGCAACCAGCCTATCGCTGCACCAGTTGTTGGTGACATCAAGTCCGCCGTTTCCCTGCTCCGCAAAGCACTGAAAGGCGCTCCAAAAGCTGACGCTGAATGGACCGGCGCTCTGAAAGCCAAAGTTGACGGCAACAAAGCCAAACTGGCTGGCAAGATGACTGCCGAAACCCCATCCGGAATGATGAACTACTCCAATTCCCTGGGCGTTGTTCGTGACTTCATGCTGGCAAATCCGGATATTTCCCTGGTTAACGAAGGCGCTAATGCACTCGACAACACTCGTATGATTGTTGACATGCTGAAACCACGCAAACGTCTTGACTCCGGTACCTGGGGTGTTATGGGTATTGGTATGGGCTACTGCGTTGCTGCAGCTGCTGTTACCGGCAAACCGGTTATCGCTGTTGAAGGCGATAGCGCATTCGGTTTCTCCGGTATGGAACTGGAAACCATCTGCCGTTACAACCTGCCAGTTACCGTTATCATCATGAACAATGGTGGTATCTATAAAGGTAACGAAGCAGATCCACAACCAGGCGTTATCTCCTGTACCCGTCTGACCCGTGGTCGTTACGACATGATGATGGAAGCATTTGGCGGTAAAGGTTATGTTGCCAATACTCCAGCAGAACTGAAAGCTGCTCTGGAAGAAGCTGTTGCTTCCGGCAAACCATGCCTGATCAACGCGATGATCGATCCAGACGCTGGTGTCGAATCTGGCCGTATCAAGAGCCTGAACGTTGTAAGTAAAGTTGGCAAGAAATAAGGTTCCTAATTCCCCTGGCATCTAAACTAAAATACAATAACCAAAAATCATACTACATGTATAAAATTAAAAAAATGGATAGCGATACTCTCTCAGGATTATTGGAAAACGTCGCCAAAAAATTCCCCGATCGCCGAGCTCTCTCCGTTTCTGGAAAATTCAATCTCACTCACGCGCGTCTTCACGATCTAATCGAACGCGCCGCTTCACGCCTTGTCTCCGACGCTGGAATCAAACCCGGCGATGTCGTTGCTCTCACCTTCCCTAACACCGTCGAGTTTGTTATAATGTTTTTAGCGGTGATAAGAGCTAGAGCCACGGCGGCGCCGTTGAACGCAGCGTACACGGCGGAGGAATTTGAGTTTTACCTCTCCGATTCAGATTCAAAGCTATTGTTAACCTCTAAAGAAGGAAACGCACCGGCTCAAGAAGCAGCTTCAAAGCTGAAAATCTCTCACGTCACCGCTACGCTGCTTGACGCTGGCTCGGACCTTGTACTATCCGTTGCGGATTCAGATTCCGTCGTTGACTCAGCGACGGAACTCGTTAATCACCCGGACGACGGTGCTCTCTTCCTCCACACTTCTGGCACTACGAGCCGTCCAAAGGGTGTACCGCTTACGCAGCTCAATCTAGCTTCATCCGTCAAGAACATTAAAGCTGTGTACAAGCTTACTGAGTCTGATTCTACGGTGATTGTTCTCCCTCTGTTCCATGTTCATGGATTGTTAGCTGGGTTGCTTAGCTCGCTTGGAGCTGGTGCTGCTGTAACTCTTCCAGCTGCTGGTAGATTCTCAGCAACAACATTTTGGCCAGATATGAAGAAGTATAACGCTACATGGTATACTGCTGTGCCGACCATTCATCAGATCATATTGGACCGCCACGCGAGCCACCCTGAAACGGAATATCCTAAACTCCGGTTCATCAGGAGTTGCAGTGCTTCTTTGGCTCCGGTGATATTGTCCAGGCTTGAGGAAGCGTTTGGAGCACCGGTGCTCGAGGCCTATGCAATGACAGAGGCAACACATTTGATGAGCTCAAACCCCTTACCAGAGGAAGGTCCACACAAGCCTGGGTCTGTTGGGAAACCGGTAGGTCAAGAAATGGCGATCCTTAATGAGAAAGGCGAGATCCAAGAGCCAAATAACAAAGGAGAGGTTTGTATAAGAGGTCCAAATGTGACCAAGGGTTACAAGAATAACCCAGAAGCCAACAAGGCAGGTTTCGAGTTTGGGTGGTTCCACACTGGTGATATCGGTTACTTTGATACCGATGGGTATTTGCATCTGGTGGGTCGGATCAAAGAGCTTATTAACCGTGGAGGTGAGAAGATATCTCCAATTGAAGTGGATGCAGTACTCTTAACGCATCCTGACGTTTCTCAGGGTGTTGCATTCGGTGTTCCTGATGAGAAATATGGGGAAGAGATTAACTGTGCGGTGATTCCAAGAGAAGGAACTACTGTAACCGAAGAGGACATTAAAGCGTTTTGTAAGAAGAATTTGGCAGCTTTCAAGGTGCCAAAGAGAGTGTTCATCACTGATAACCTCCCCAAAACTGCCTCTGGTAAGATTCAGCGCCGTATCGTCGCACAACATTTCCTTGAGAAGCCCTGAGGTTCCTAAAATGCCTGGCATCAACTACCTCAATTAATAATCAAACCATCTGAACAATGAATAAATTTAAAAAAATGACAAGTGCCGCTACTGTTACTGCTTCGTTCAACGATACTTTTAGCGTATCCGATAATGTCGCCGTTATTGTTCCTGAAACGGACACTCAGGTGACCTACAGGGATCTATCCCACATGGTGGGTCACTTCCAGACCATGTTCACAAATCCTAATTCTCCATTGTACGGAGCTGTTTTCAGACAAGATACAGTGGCGATATCCATGCGTAATGGGCTGGAATTTATCGTCGCTTTCCTCGGTGCTACTATGGACGCTAAAATTGGCGCGCCCTTGAATCCCAATTATAAGGAAAAGGAGTTCAATTTTTATTTGAATGACCTGAAATCTAAGGCGATTTGCGTCCCAAAGGGTACCACAAAGTTACAGAGTTCTGAAATTCTAAAATCTGCCTCCACGTTTGGATGTTTTATCGTAGAGCTGGCCTTCGATGCGACCAGGTTTAGGGTAGAGTATGATATATACTCTCCAGAGGACAACTACAAAAGGGTTATTTACCGGTCTTTGAACAACGCCAAATTTGTCAACACAAATCCCGTTAAATTCCCTGGGTTTGCCCGTTCCAGTGACGTTGCCCTGATTTTGCATACCAGTGGTACCACCTCCACTCCAAAAACGGTGCCTTTGTTACATTTGAACATTGTGAGAAGCACGTTGAACATTGCTAACACTTACAAGCTAACGCCCTTGGACAGATCTTATGTCGTGATGCCTCTTTTCCACGTCCATGGGTTAATTGGTGTTTTACTTTCCACTTTTAGAACTCAGGGTTCTGTTGTGGTTCCCGATGGATTCCATCCAAAGTTATTCTGGGACCAATTTGTTAAGTACAACTGTAATTGGTTCAGTTGCGTTCCCACAATAAGCATGATTATGCTGAACATGCCCAAACCAAACCCTTTCCCACACATTAGATTCATCAGATCGTGTTCTTCTGCTTTGGCTCCAGCAACGTTCCATAAGCTGGAGAAGGAATTCAATGCACCTGTCTTGGAGGCCTATGCGATGACCGAAGCATCACATCAAATGACCTCAAACAATCTGCCTCCAGGAAAGAGAAAGCCTGGTACTGTGGGCCAGCCACAAGGAGTCACCGTCGTCATTCTAGATGACAATGACAATGTCTTGCCCCCGGGCAAAGTCGGCGAAGTTTCCATCAGAGGCGAAAACGTCACTTTGGGGTATGCTAATAATCCAAAAGCTAACAAGGAGAACTTCACCAAGAGAGAGAACTATTTCAGAACCGGTGACCAAGGTTATTTCGACCCTGAGGGGTTTTTGGTCCTTACAGGCAGAATCAAAGAGCTTATCAACAGGGGTGGTGAAAAGATTTCACCCATTGAGCTCGACGGCATTATGCTATCGCATCCAAAGATCGATGAAGCCGTTGCATTTGGTGTTCCCGACGATATGTACGGCCAAGTAGTTCAAGCCGCCATTGTTTTGAAGAAGGGAGAAAAAATGACCTACGAAGAACTGGTGAACTTCTTAAAGAAGCACCTAGCCTCTTTCAAAATTCCAACCAAGGTGTACTTTGTTGATAAGCTACCAAAAACCGCTACAGGTAAAATCCAGAGAAGAGTTATCGCAGAAACTTTTGCTAAGAGCAGCAGAAATAAGAGTAAGTTGTAGGGTTCCTAAAAACCCTGGCATCACCTAAAAATTATAAATAAATTAATAAACTTTTATTAAAAAAAAATTACAAAATGAATAATCCACAAACAGGACAATCAACAGGCCTCTTGGGCAATCGTTGGTTCTACTTGGTATTAGCAGTTTTGCTGATGTGTATGATCTCGGGTGTCCAATATTCCTGGACACTGTACGCTAACCCGGTTAAAGACAACCTTGGCGTTTCTTTGGCTGCGGTTCAGACGGCTTTCACACTCTCTCAGGTCATTCAAGCTGGTTCTCAGCCTGGTGGTGGTTACTTCGTTGATAAATTCGGTCCAAGAATTCCATTGATGTTCGGTGGTGCGATGGTTCTCGCTGGCTGGACCTTCATGGGTATGGTTGACAGTGTTCCTGCTCTGTATGCTCTTTATACTCTGGCCGGTGCAGGTGTTGGTATCGTTTACGGTATCGCGATGAACACGGCTAACAGATGGTTCCCGGACAAACGCGGTCTGGCTTCCGGTTTCACCGCTGCCGGTTACGGTCTGGGTGTTCTGCCGTTCCTGCCACTGATCAGCTCCGTTCTGAAAGTTGAAGGTGTTGGCGCAGCATTCATGTACACCGGTTTGATCATGGGTATCCTGATTATCCTGATCGCTTTCGTTATCCGTTTCCCTGGCCAGCAAGGCGCCAAAAAACAAATCGTTGTTACCGACAAGGATTTCAATTCTGGCGAAATGCTGAGAACACCACAATTCTGGGTTCTGTGGACCGCATTCTTTTCCGTTAACTTTGGTGGTTTGCTGCTGGTTGCCAACAGCGTCCCTTACGGTCGCAGCCTCGGTCTTGCCGCAGGTGTGCTGACGATCGGTGTTTCGATCCAGAACCTGTTCAATGGTGGTTGCCGTCCTTTCTGGGGTTTCGTTTCCGATAAAATCGGCCGTTACAAAACCATGTCCGTCGTTTTCGGTATCAATGCTGTTGTTCTCGCACTTTTCCCGACGATTGCTGCCTTGGGCGATGTAGCCTTTATCGCCATGTTGGCAATCGCATTCTTCACATGGGGTGGTAGCTACGCTCTGTTCCCATCGACCAACAGCGATATTTTCGGTACGGCATACTCTGCCAGAAACTATGGTTTCTTCTGGGCTGCAAAAGCAACTGCCTCGATCTTCGGTGGTGGTCTGGGTGCTGCAATTGCAACCAACTTCGGATGGAATACCGCTTTCCTGATTACTGCGATTACTTCTTTCATCGCATTTGCTCTGGCTACCTTCGTTATTCCAAGAATGGGCCGTCCAGTCAAGAAAATGGTCAAATTGTCTCCAGAAGAAAAAGCTGTACATTAAGGTTCCTAGCTGATTAGAAGGCCATCCTGACGGATGGCCTTTTTTTTGACTCTGTTGTGTCTTCTTTTAGGTGTGGAATAATTTTCCAACCATCACTTTATCTGTCTCCCGAACAGCACCTTATTCAGCCATCGGTTCACATACACGTTCACGAAAGCACATCCTACTCCGATAGCGGCTCCTGCCACTACATCACTGGGATAGTGCACCCCTTGGTTCATCCGGGCAAACCCTACTCCGCAAGCCCATACGGCTGAAGGGGCGATAACATACCACTTAGGATAAGTGATGGAGAGTGAGGTGGCTAAAGAAAAAGCTGCCGCAGTGTGTCCTGAAGGGAATGACGGACTATCGGCATCCGGTGCTCCGTATGCATGAATCTTATCCGGATACTTTACAAAAGGCCGGTCACGACCTATTATATGTTTGGCGGCGTATGTAATCCCCACTGCTTCAATCACACTGGTTCCTATGTAAATGGCATCTTTCAGCATCGGCTGGTCTTTCTTTATCAATGCATAGAGTCCCATGGCTGTAGGTATTCCTACGGGTAGAATCACTCCGCTAGCGGACAATCCGCGGCTCAGTCCGTGTACATCCCATCGGTTGATGGTTTTCAACGTGTTGATGTCTATATTCTGTGCGCTGAGGCTGAATGTAAAACAGGCTGTCAGTATAAGAAAAATAATCCTTTGGCGTTTCATTTAGTTTGGCATTTGTTCAGTGCAAAGATAGGAAAATAATGTAATTCGCATTTACCCTTCCCCGAATTAAAGAATAATTTCCATAATTCCTTGTATTATCGCTAGAATTCCTTTACTTTTGCATCGTGAATACACAGCTTTTAACAGAATTTTTAGTTCTAACAATTAAATAATTTAAATTCAATCATTTATGTGGTTAAGTAATTCATCTATTGGAAGGAAAGTGGTGATGAGTGTCACAGGTATCGCCCTTGTCCTGTTTCTGACGTTTCACATGGCGATGAACCTTGTTGCGCTCTTCTCGGAAGATGCTTACAACATGATTTGTGAATTCTTGGGAGCTAACTGGTACGCGTTGGTTGCAACTGCCGGGCTGGCTGCTCTGTTTGTAATTCACATCATCTACGCTTTCTGGTTGACCATGCAGAACCGTGCAGCTCGCGGTAGCGAACGTTATGCAGTGAACGTTAAACCTAAAACTGTGGAATGGGCATCTCAGAACATGCTTGTCCTGGGTATTGTCGTTATTGCCGGATTGGCTCTTCATTTTGCCAATTTCTGGTACAAAATGCAGTTTGCCGAAATTATCGGTGTTCATGATTTGGGCGCATTCGGTCCTACAGACGGTGCCGCTTACATTCGTGAAACATTCGGATGCCCTGTATTTACAGTGCTTTACCTCATTTGGTTAGCAGCTTTGTGGTTCCACCTGACTCACGGATTCTGGAGTGCTTTGCAGACTTTGGGCTGGAGCAATAAAATCTGGTTCGAGCGTTGGAAGACTATTGGTAACATCTACTCTACTTTGGTAGTTCTTGGTTTTGCAGCAGTAGTCGTTATCTACTTCGCTATGACTGGCGCGCCTGATAGGTGGGCTGCCCTTCCTGGTTGGCTTGGTTTCATCAGCCATCCGCTTGCCCTCATCTGTTACGCCGGCGGTAGCCGGCCAGCCTCGCAGAGCAGGATTCCCGTTGAGCACCGCCAGGTGCGAATAAGGGACAGTGAAGAAGGAACACCCGCTCGCGGGTGGGCCTACTTCACCTATCCTGCCCGGCTGACGCCGTTGGATACACCAAGGAAAGTCTACACGAACCCTTTGGCAAAATCCTGTATATCGTGCGAAAAAGGATGGATATACCGAAAAAATCGCTATAATGACCCCGAAGCAGGGTTATGCAGCGGAAAAGCGGGATTAAAAGTCGGGGATTGGTGAACAAAAAGGTGTTTCTCTCTTTAAGAGAAATATCGTTTTGCTAAACAGTTGATATTGAGGTATCATTTTATCGTAAAAGACATTTTTGCTCAACAATTGCTTGACGGAAATCAACAAATTTTAGCATTTTGTAAAAAAGTCGCTATATAATTTGGTGAATTGGAGTTATTTTCATATTTTTGCATCCCGAAGAGTTTCTCTTAAAGAGAGAAACATCTTTTGCATACCTTTTCCGACCGAATTTTTATGTCGTAAAGAGGGGCTTTGCAGGGGGTGGACTCAGAAAGATGAGAATAGATGACTATTGTAGTTGAAACACATAGAAAGTTGCTGATATACAGACCGATACGCATATCGGGATGAACCATGAGTACGTTCTTTTCTCAAAAAACATAAATATTCGAAAAGAGATGCAATAAATTAAGGAGAGGTTATAATGAACAAAGTAAATATAAAAGATAGTCAAAATTTTATTACTTCAAAATATCACATAGAAAAAATAATGAATTGCATAAGTTTAGATGAAAAAGATAACATCTTTGAAATAGGTGCAGGGAAAGGTCATTTTACTGCTGGATTGGTAAAGAGATGTAATTTTGTAACGGCGATAGAAATTGATTCTAAATTATGTGAGGTAACTCGTAATAAGCTCTTAAATTATCCTAACTATCAAATAGTAAATGATGATATACTGAAATTTACATTTCCTAGCCACAATCCATATAAAATATTTGGCAGCATACCTTACAACATAAGCACAAATATAATTCGAAAAATTGTTTTTGAAAGTTCAGCCACAATAAGTTATTTAATAGTGGAATATGGTTTTGCTAAAATGTTATTAGATACAAACAGATCACTAGCATTGCTGTTAATGGCAGAGGTAGATATTTCTATATTAGCAAAAATTCCTAGGTATTATTTCCATCCAAAACCTAAAGTGGATAGCACATTAATTGTATTAAAAAGAAAGCCAGCAAAAATGGCATTTAAAGAGAGAAAAAAATATGAAACTTTTGTAATGAAATGGGTTAACAAAGAGTACGAAAAACTGTTTACAAAAAATCAATTTAATAAAGCTTTAAAACATGCGAGAATATATGATATAAACAATATTAGTTTCGAACAATTTGTATCGCTATTTAATAGTTATAAAATATTTAACGGCTAAAAACAATAGGCCACATGCAACTGTAAATGTTTACGCGGGTACCGACACCGCGGTGGAGGGGAATTGTGTTACAACCAATTAACCAATTCTGATTAGAAAAACTCATCGAGCATCAAATGAAACTGCAATTTATTCATATCAGGATTATCAATACCATATTTTTGAAAAAGCCGTTTCTGTAATGAAGGAGAAAACTCACCGAGGCAGTTCCATAGGATGGCAAGATCCTGGTATCGGTCTGCGATTCCGACTCGTCCAACATCAATACAACCTATTAATTTCCCCTCGTCAAAAATAAGGTTATCAAGTGAGAAATCACCATGAGTGACGACTGAATCCGGTGAGAATGGCAAAAGCTTATGCATTTCTTTCCAGACTTGTTCAACAGGCCAGCCATTACGCTCGTCATCAAAATCACTCGCATCAACCAAACCGTTATTCATTCGTGATTGCGCCTGAGCGAGGCGAAATACGCGATCGCTGTTAAAAGGACAATTACAAACAGGAATCGAATGCAACCGGCGCAGGAACACTGCCAGCGCATCAACAATATTTTCACCTGAATCAGGATATTCTTCTAATACCTGGAATGCTGTTTTCCCGGGGATCGCAGTGGTGAGTAACCATGCATCATCAGGAGTACGGATAAAATGCTTGATGGTCGGAAGAGGCATAAATTCCGTCAGCCAGTTTAGTCTGACCATCTCATCTGTAACATCATTGGCAACGCTACCTTTGCCATGTTTCAGAAACAACTCTGGCGCATCGGGCTTCCCATACAATCGATAGATTGTCGCACCTGATTGCCCGACATTATCGCGAGCCCATTTATACCCATATAAATCAGCATCCATGTTGGAATTTAATCGCGGCCTGGAGCAAGACGTTTCCCGTTGAATATGGCTCATAACACCCCTTGTATTACTGTTTATGTAAGCAGACAGTTTTATTGTTCATGATGATATATTTTTATCTTGTGCAATGTAACATCAGAGATTTTGAGACACAACGTGGCTTTGTTGAATAAATCGAACTTTTGCTGAGTTGAAGGATCAGAGCCTACGTTCCGAATACGGTCAAAAAAAAGGCCATCCGTCAGGATGGCCTTCGCATTAATATGCCGCTTCGAATTCTTTTAGGAAGCGTGTATCGTTTTCAGAGAACATACGGAGGTCTTTCACCTGATATTTCAGGTTTGTGATACGCTCGATACCCATACCGAGTCCATAACCGCTGTATATTTTGCTGTCTATACCATTTGATTCAAGTACGTTCGGGTCTACCATACCGCAACCGAGGATTTCTACCCAGCCGGTGTGTTTACAGAACGGACATCCTTTACCGCCGCAGATATTACAGCTGATATCCATTTCCGCACTTGGTTCAGCAAACGGGAAGTAAGACGGACGCAGACGGATCTTTGTATCAGCACCGAACATTTCTTTGGCAAAGAGCAGCAATACCTGCTTCAAGTCGGTGAATGATACGTTTTTATCTACATACAGCGCTTCTACCTGATGGAAGAAACAGTGTGCGCGATAGCTGATAGCTTCGTTACGATATACACGTCCCGGACAGATGATGCGGATAGGAGGCTGTGAAGTTTCCATCACACGAGTCTGTACAGAAGAAGTATGTGTACGCAATACTACGTCCGGGTGAGCTTCGATAAAGAAAGTGTCCTGCATATCGCGTGCCGGATGATCTTCGGCAAAGTTCAGTGCCGAGAACACGTGCCAGTCATCTTCAATTTCCGGACCTTCGGCAATGCTGAATCCCAGACGGGCAAAGATATCAATGATTTCGTTCTTTACAATGGTGAGCGGGTGGCGTGTACCGAGTTCTACAGGATAAGCCGAACGCGTCAAATCCAGTCCGTCACAATCGTTGTCCTGACTTTCAAACATTTCTTTCAGCGCGTTGATTTTGTCCTGCGCTTTTGTTTTCAGTTCATTCAGTCTCATGCCGACTTCTTTTTTCTGTTCGGCAGCTACATTACGGAAATCTGCCATTAAGTCGTTAATGGCTCCCTTCTTACTTAGGTATTTGATGCGGAGAGCTTCGAGTTCTTCGGCATTGGAGGCGTGTAAGGCTTCCACCTCTTTCAGAAGTTGTTCAATCTTAGCTATCATTTTTTAATATTTTTAGCGGCCCCGTTAAACAAAATTATTTGTAGAGGCTGTTTCGTCCTCACGGACTCATCAGACCGGAAAGCACATCCGGTGACAGCTCAGGCTACTTTGTTTCTTTCGACACTGCAAATATAAGAACATTATTTGAAAGTTCAAGTGAAACTTTAAATTTTAACAATAGATTAACCATTGCAAACAAAACAAAAAAAAGGTAGCCCAATTGTAAAACGAAAGGCCCAGTCTTTCGACTGAGCCTTTCGTTTTATCCTACAGTCGCTCGGCGATCGAAGGCTTCGGAAAAAAAAGGCCATCCGTCAGGATGGCCTTCGCATTAATATGCCGCTTCGAATTCTTTTAGGAAGCGTGTATCGTTTTCAGAGAACATACGGAGGTCTTTCACCTGATATTTCAGGTTTGTGATACGCTCGATACCCATACCGAGTCCATAACCGCTGTATATTTTGCTGTCTATACCATTTGATTCAAGTACGTTCGGGTCTACCATACCGCAACCGAGGATTTCTACCCAGCCGGTGTGTTTACAGAACGGACATCCTTTACCGCCGCAGATATTACAGCTGATATCCATTTCCGCACTTGGTTCAGCAAACGGGAAGTAAGACGGACGCAGACGGATCTTTGTATCAGCACCGAACATTTCTTTGGCAAAGAGCAGCAATACCTGCTTCAAGTCGGTGAATGATACGTTTTTATCTACATACAGCGCTTCTACCTGATGGAAGAAACAGTGTGCGCGATAGCTGATAGCTTCGTTACGATATACACGTCCCGGACAGATGATGCGGATAGGAGGCTGTGAAGTTTCCATCACACGAGTCTGTACAGAAGAAGTATGTGTACGCAATACTACGTCCGGGTGAGCTTCGATAAAGAAAGTGTCCTGCATATCGCGTGCCGGATGATCTTCGGCAAAGTTCAGTGCCGAGAACACGTGCCAGTCATCTTCAATTTCCGGACCTTCGGCAATGCTGAATCCCAGACGGGCAAAGATATCAATGATTTCGTTCTTTACAATGGTGAGCGGGTGGCGTGTACCGAGTTCTACAGGATAAGCCGAACGCGTCAAATCCAGTCCGTCACAATCGTTGTCCTGACTTTCAAACATTTCTTTCAGCGCGTTGATTTTGTCCTGCGCTTTTGTTTTCAGTTCATTCAGTCTCATGCCGACTTCTTTTTTCTGTTCGGCAGCTACATTACGGAAATCTGCCATTAAGTCGTTAATGGCTCCCTTCTTACTTAGGTATTTGATGCGGAGAGCTTCGAGTTCTTCGGCATTGGAGGCGTGTAAGGCTTCCACCTCTTTCAGAAGTTGTTCAATCTTAGCTATCATTTTTTAATATTTTTAGCGGCCCCGTTAAACAAAATTATTTGTAGAGGCTGTTTCGTCCTCACGGACTCATCAGACCGGAAAGCACATCCGGTGACAGCTCAGGCTACTTTGTTTCTTTCGACACTGCAAATATAAGAACATTATTTGAAAGTTCAAGTGAAACTTTAAATTTTAACAATAGATTAACCATTGCAAACAAAACAAAAAAAAGGTAGCCCAATTGTAAAACGAAAGGCCCAGTCTTTCGACTGAGCCTTTCGTTTTATCCTAGGATCAGCTGTACGTACTCGCAGTTCAACCTGTTGATAGTACGTACTAAGCTCTCATGTTTCACGTACTAAGCTCTCATGTTTAACGTACTAAGCTCTCATGTTTAACGAACTAAACCCTCATGGCTAACGTACTAAGCTCTCATGGCTAACGTACTAAGCTCTCATGTTTCACGTACTAAGCTCTCATGTTTGAACAATAAAATTAATATAAATCAGCAACTTAAATAGCCTCTAAGGTTTTAAGTTTTATAAGAAAAAAAAGAATATATAAGGCTTTTAAAGCTTTTAAGGTTTAACGGTTGTGGACAACAAGCCAGGGATGTAACGCACTGAGAAGCCCTTAGAGCCTCTCAAAGCAATTTTGAGTGACACAGGAACACTTAACGGCTGACATGGGGCGGCCGCGTAG
SEQ ID NO:96(核糖体结合位点RBS1和At_AAE3开放读码框)：
GCATCTAAACTAAAATACAATAACCAAAAATCATTACAAATGAAAAAAAATAATAAAATGGATAGCGATACTCTCTCAGGATTATTGGAAAACGTCGCCAAAAAATTCCCCGATCGCCGAGCTCTCTCCGTTTCTGGAAAATTCAATCTCACTCACGCGCGTCTTCACGATCTAATCGAACGCGCCGCTTCACGCCTTGTCTCCGACGCTGGAATCAAACCCGGCGATGTCGTTGCTCTCACCTTCCCTAACACCGTCGAGTTTGTTATAATGTTTTTAGCGGTGATAAGAGCTAGAGCCACGGCGGCGCCGTTGAACGCAGCGTACACGGCGGAGGAATTTGAGTTTTACCTCTCCGATTCAGATTCAAAGCTATTGTTAACCTCTAAAGAAGGAAACGCACCGGCTCAAGAAGCAGCTTCAAAGCTGAAAATCTCTCACGTCACCGCTACGCTGCTTGACGCTGGCTCGGACCTTGTACTATCCGTTGCGGATTCAGATTCCGTCGTTGACTCAGCGACGGAACTCGTTAATCACCCGGACGACGGTGCTCTCTTCCTCCACACTTCTGGCACTACGAGCCGTCCAAAGGGTGTACCGCTTACGCAGCTCAATCTAGCTTCATCCGTCAAGAACATTAAAGCTGTGTACAAGCTTACTGAGTCTGATTCTACGGTGATTGTTCTCCCTCTGTTCCATGTTCATGGATTGTTAGCTGGGTTGCTTAGCTCGCTTGGAGCTGGTGCTGCTGTAACTCTTCCAGCTGCTGGTAGATTCTCAGCAACAACATTTTGGCCAGATATGAAGAAGTATAACGCTACATGGTATACTGCTGTGCCGACCATTCATCAGATCATATTGGACCGCCACGCGAGCCACCCTGAAACGGAATATCCTAAACTCCGGTTCATCAGGAGTTGCAGTGCTTCTTTGGCTCCGGTGATATTGTCCAGGCTTGAGGAAGCGTTTGGAGCACCGGTGCTCGAGGCCTATGCAATGACAGAGGCAACACATTTGATGAGCTCAAACCCCTTACCAGAGGAAGGTCCACACAAGCCTGGGTCTGTTGGGAAACCGGTAGGTCAAGAAATGGCGATCCTTAATGAGAAAGGCGAGATCCAAGAGCCAAATAACAAAGGAGAGGTTTGTATAAGAGGTCCAAATGTGACCAAGGGTTACAAGAATAACCCAGAAGCCAACAAGGCAGGTTTCGAGTTTGGGTGGTTCCACACTGGTGATATCGGTTACTTTGATACCGATGGGTATTTGCATCTGGTGGGTCGGATCAAAGAGCTTATTAACCGTGGAGGTGAGAAGATATCTCCAATTGAAGTGGATGCAGTACTCTTAACGCATCCTGACGTTTCTCAGGGTGTTGCATTCGGTGTTCCTGATGAGAAATATGGGGAAGAGATTAACTGTGCGGTGATTCCAAGAGAAGGAACTACTGTAACCGAAGAGGACATTAAAGCGTTTTGTAAGAAGAATTTGGCAGCTTTCAAGGTGCCAAAGAGAGTGTTCATCACTGATAACCTCCCCAAAACTGCCTCTGGTAAGATTCAGCGCCGTATCGTCGCACAACATTTCCTTGAGAAGCCC
SEQ ID NO:97(核糖体结合位点RBS2和At_AAE3开放读码框):
GCATCTAAACTAAAATACAATAACCAAAAATCATGTTTAACAAATAAAAATAATAAAATGGATAGCGATACTCTCTCAGGATTATTGGAAAACGTCGCCAAAAAATTCCCCGATCGCCGAGCTCTCTCCGTTTCTGGAAAATTCAATCTCACTCACGCGCGTCTTCACGATCTAATCGAACGCGCCGCTTCACGCCTTGTCTCCGACGCTGGAATCAAACCCGGCGATGTCGTTGCTCTCACCTTCCCTAACACCGTCGAGTTTGTTATAATGTTTTTAGCGGTGATAAGAGCTAGAGCCACGGCGGCGCCGTTGAACGCAGCGTACACGGCGGAGGAATTTGAGTTTTACCTCTCCGATTCAGATTCAAAGCTATTGTTAACCTCTAAAGAAGGAAACGCACCGGCTCAAGAAGCAGCTTCAAAGCTGAAAATCTCTCACGTCACCGCTACGCTGCTTGACGCTGGCTCGGACCTTGTACTATCCGTTGCGGATTCAGATTCCGTCGTTGACTCAGCGACGGAACTCGTTAATCACCCGGACGACGGTGCTCTCTTCCTCCACACTTCTGGCACTACGAGCCGTCCAAAGGGTGTACCGCTTACGCAGCTCAATCTAGCTTCATCCGTCAAGAACATTAAAGCTGTGTACAAGCTTACTGAGTCTGATTCTACGGTGATTGTTCTCCCTCTGTTCCATGTTCATGGATTGTTAGCTGGGTTGCTTAGCTCGCTTGGAGCTGGTGCTGCTGTAACTCTTCCAGCTGCTGGTAGATTCTCAGCAACAACATTTTGGCCAGATATGAAGAAGTATAACGCTACATGGTATACTGCTGTGCCGACCATTCATCAGATCATATTGGACCGCCACGCGAGCCACCCTGAAACGGAATATCCTAAACTCCGGTTCATCAGGAGTTGCAGTGCTTCTTTGGCTCCGGTGATATTGTCCAGGCTTGAGGAAGCGTTTGGAGCACCGGTGCTCGAGGCCTATGCAATGACAGAGGCAACACATTTGATGAGCTCAAACCCCTTACCAGAGGAAGGTCCACACAAGCCTGGGTCTGTTGGGAAACCGGTAGGTCAAGAAATGGCGATCCTTAATGAGAAAGGCGAGATCCAAGAGCCAAATAACAAAGGAGAGGTTTGTATAAGAGGTCCAAATGTGACCAAGGGTTACAAGAATAACCCAGAAGCCAACAAGGCAGGTTTCGAGTTTGGGTGGTTCCACACTGGTGATATCGGTTACTTTGATACCGATGGGTATTTGCATCTGGTGGGTCGGATCAAAGAGCTTATTAACCGTGGAGGTGAGAAGATATCTCCAATTGAAGTGGATGCAGTACTCTTAACGCATCCTGACGTTTCTCAGGGTGTTGCATTCGGTGTTCCTGATGAGAAATATGGGGAAGAGATTAACTGTGCGGTGATTCCAAGAGAAGGAACTACTGTAACCGAAGAGGACATTAAAGCGTTTTGTAAGAAGAATTTGGCAGCTTTCAAGGTGCCAAAGAGAGTGTTCATCACTGATAACCTCCCCAAAACTGCCTCTGGTAAGATTCAGCGCCGTATCGTCGCACAACATTTCCTTGAGAAGCCC
SEQ ID NO:98(核糖体结合位点RBS3和At_AAE3开放读码框):
GCATCTAAACTAAAATACAATAACCAAAAATCATCTATGCGTAAAAAAAATTATAAAATGGATAGCGATACTCTCTCAGGATTATTGGAAAACGTCGCCAAAAAATTCCCCGATCGCCGAGCTCTCTCCGTTTCTGGAAAATTCAATCTCACTCACGCGCGTCTTCACGATCTAATCGAACGCGCCGCTTCACGCCTTGTCTCCGACGCTGGAATCAAACCCGGCGATGTCGTTGCTCTCACCTTCCCTAACACCGTCGAGTTTGTTATAATGTTTTTAGCGGTGATAAGAGCTAGAGCCACGGCGGCGCCGTTGAACGCAGCGTACACGGCGGAGGAATTTGAGTTTTACCTCTCCGATTCAGATTCAAAGCTATTGTTAACCTCTAAAGAAGGAAACGCACCGGCTCAAGAAGCAGCTTCAAAGCTGAAAATCTCTCACGTCACCGCTACGCTGCTTGACGCTGGCTCGGACCTTGTACTATCCGTTGCGGATTCAGATTCCGTCGTTGACTCAGCGACGGAACTCGTTAATCACCCGGACGACGGTGCTCTCTTCCTCCACACTTCTGGCACTACGAGCCGTCCAAAGGGTGTACCGCTTACGCAGCTCAATCTAGCTTCATCCGTCAAGAACATTAAAGCTGTGTACAAGCTTACTGAGTCTGATTCTACGGTGATTGTTCTCCCTCTGTTCCATGTTCATGGATTGTTAGCTGGGTTGCTTAGCTCGCTTGGAGCTGGTGCTGCTGTAACTCTTCCAGCTGCTGGTAGATTCTCAGCAACAACATTTTGGCCAGATATGAAGAAGTATAACGCTACATGGTATACTGCTGTGCCGACCATTCATCAGATCATATTGGACCGCCACGCGAGCCACCCTGAAACGGAATATCCTAAACTCCGGTTCATCAGGAGTTGCAGTGCTTCTTTGGCTCCGGTGATATTGTCCAGGCTTGAGGAAGCGTTTGGAGCACCGGTGCTCGAGGCCTATGCAATGACAGAGGCAACACATTTGATGAGCTCAAACCCCTTACCAGAGGAAGGTCCACACAAGCCTGGGTCTGTTGGGAAACCGGTAGGTCAAGAAATGGCGATCCTTAATGAGAAAGGCGAGATCCAAGAGCCAAATAACAAAGGAGAGGTTTGTATAAGAGGTCCAAATGTGACCAAGGGTTACAAGAATAACCCAGAAGCCAACAAGGCAGGTTTCGAGTTTGGGTGGTTCCACACTGGTGATATCGGTTACTTTGATACCGATGGGTATTTGCATCTGGTGGGTCGGATCAAAGAGCTTATTAACCGTGGAGGTGAGAAGATATCTCCAATTGAAGTGGATGCAGTACTCTTAACGCATCCTGACGTTTCTCAGGGTGTTGCATTCGGTGTTCCTGATGAGAAATATGGGGAAGAGATTAACTGTGCGGTGATTCCAAGAGAAGGAACTACTGTAACCGAAGAGGACATTAAAGCGTTTTGTAAGAAGAATTTGGCAGCTTTCAAGGTGCCAAAGAGAGTGTTCATCACTGATAACCTCCCCAAAACTGCCTCTGGTAAGATTCAGCGCCGTATCGTCGCACAACATTTCCTTGAGAAGCCC
SEQ ID NO:99(核糖体结合位点RBS4和At_AAE3开放读码框):
GCATCTAAACTAAAATACAATAACCAAAAATCATACTACATGTATAAAATTAAAAAAATGGATAGCGATACTCTCTCAGGATTATTGGAAAACGTCGCCAAAAAATTCCCCGATCGCCGAGCTCTCTCCGTTTCTGGAAAATTCAATCTCACTCACGCGCGTCTTCACGATCTAATCGAACGCGCCGCTTCACGCCTTGTCTCCGACGCTGGAATCAAACCCGGCGATGTCGTTGCTCTCACCTTCCCTAACACCGTCGAGTTTGTTATAATGTTTTTAGCGGTGATAAGAGCTAGAGCCACGGCGGCGCCGTTGAACGCAGCGTACACGGCGGAGGAATTTGAGTTTTACCTCTCCGATTCAGATTCAAAGCTATTGTTAACCTCTAAAGAAGGAAACGCACCGGCTCAAGAAGCAGCTTCAAAGCTGAAAATCTCTCACGTCACCGCTACGCTGCTTGACGCTGGCTCGGACCTTGTACTATCCGTTGCGGATTCAGATTCCGTCGTTGACTCAGCGACGGAACTCGTTAATCACCCGGACGACGGTGCTCTCTTCCTCCACACTTCTGGCACTACGAGCCGTCCAAAGGGTGTACCGCTTACGCAGCTCAATCTAGCTTCATCCGTCAAGAACATTAAAGCTGTGTACAAGCTTACTGAGTCTGATTCTACGGTGATTGTTCTCCCTCTGTTCCATGTTCATGGATTGTTAGCTGGGTTGCTTAGCTCGCTTGGAGCTGGTGCTGCTGTAACTCTTCCAGCTGCTGGTAGATTCTCAGCAACAACATTTTGGCCAGATATGAAGAAGTATAACGCTACATGGTATACTGCTGTGCCGACCATTCATCAGATCATATTGGACCGCCACGCGAGCCACCCTGAAACGGAATATCCTAAACTCCGGTTCATCAGGAGTTGCAGTGCTTCTTTGGCTCCGGTGATATTGTCCAGGCTTGAGGAAGCGTTTGGAGCACCGGTGCTCGAGGCCTATGCAATGACAGAGGCAACACATTTGATGAGCTCAAACCCCTTACCAGAGGAAGGTCCACACAAGCCTGGGTCTGTTGGGAAACCGGTAGGTCAAGAAATGGCGATCCTTAATGAGAAAGGCGAGATCCAAGAGCCAAATAACAAAGGAGAGGTTTGTATAAGAGGTCCAAATGTGACCAAGGGTTACAAGAATAACCCAGAAGCCAACAAGGCAGGTTTCGAGTTTGGGTGGTTCCACACTGGTGATATCGGTTACTTTGATACCGATGGGTATTTGCATCTGGTGGGTCGGATCAAAGAGCTTATTAACCGTGGAGGTGAGAAGATATCTCCAATTGAAGTGGATGCAGTACTCTTAACGCATCCTGACGTTTCTCAGGGTGTTGCATTCGGTGTTCCTGATGAGAAATATGGGGAAGAGATTAACTGTGCGGTGATTCCAAGAGAAGGAACTACTGTAACCGAAGAGGACATTAAAGCGTTTTGTAAGAAGAATTTGGCAGCTTTCAAGGTGCCAAAGAGAGTGTTCATCACTGATAACCTCCCCAAAACTGCCTCTGGTAAGATTCAGCGCCGTATCGTCGCACAACATTTCCTTGAGAAGCCC
SEQ ID NO:100(核糖体结合位点RBS5和At_AAE3开放读码框):
GCATCTAAACTAAAATACAATAACCAAAAATCATTTTCACAATATAAAAATAACAAAATGGATAGCGATACTCTCTCAGGATTATTGGAAAACGTCGCCAAAAAATTCCCCGATCGCCGAGCTCTCTCCGTTTCTGGAAAATTCAATCTCACTCACGCGCGTCTTCACGATCTAATCGAACGCGCCGCTTCACGCCTTGTCTCCGACGCTGGAATCAAACCCGGCGATGTCGTTGCTCTCACCTTCCCTAACACCGTCGAGTTTGTTATAATGTTTTTAGCGGTGATAAGAGCTAGAGCCACGGCGGCGCCGTTGAACGCAGCGTACACGGCGGAGGAATTTGAGTTTTACCTCTCCGATTCAGATTCAAAGCTATTGTTAACCTCTAAAGAAGGAAACGCACCGGCTCAAGAAGCAGCTTCAAAGCTGAAAATCTCTCACGTCACCGCTACGCTGCTTGACGCTGGCTCGGACCTTGTACTATCCGTTGCGGATTCAGATTCCGTCGTTGACTCAGCGACGGAACTCGTTAATCACCCGGACGACGGTGCTCTCTTCCTCCACACTTCTGGCACTACGAGCCGTCCAAAGGGTGTACCGCTTACGCAGCTCAATCTAGCTTCATCCGTCAAGAACATTAAAGCTGTGTACAAGCTTACTGAGTCTGATTCTACGGTGATTGTTCTCCCTCTGTTCCATGTTCATGGATTGTTAGCTGGGTTGCTTAGCTCGCTTGGAGCTGGTGCTGCTGTAACTCTTCCAGCTGCTGGTAGATTCTCAGCAACAACATTTTGGCCAGATATGAAGAAGTATAACGCTACATGGTATACTGCTGTGCCGACCATTCATCAGATCATATTGGACCGCCACGCGAGCCACCCTGAAACGGAATATCCTAAACTCCGGTTCATCAGGAGTTGCAGTGCTTCTTTGGCTCCGGTGATATTGTCCAGGCTTGAGGAAGCGTTTGGAGCACCGGTGCTCGAGGCCTATGCAATGACAGAGGCAACACATTTGATGAGCTCAAACCCCTTACCAGAGGAAGGTCCACACAAGCCTGGGTCTGTTGGGAAACCGGTAGGTCAAGAAATGGCGATCCTTAATGAGAAAGGCGAGATCCAAGAGCCAAATAACAAAGGAGAGGTTTGTATAAGAGGTCCAAATGTGACCAAGGGTTACAAGAATAACCCAGAAGCCAACAAGGCAGGTTTCGAGTTTGGGTGGTTCCACACTGGTGATATCGGTTACTTTGATACCGATGGGTATTTGCATCTGGTGGGTCGGATCAAAGAGCTTATTAACCGTGGAGGTGAGAAGATATCTCCAATTGAAGTGGATGCAGTACTCTTAACGCATCCTGACGTTTCTCAGGGTGTTGCATTCGGTGTTCCTGATGAGAAATATGGGGAAGAGATTAACTGTGCGGTGATTCCAAGAGAAGGAACTACTGTAACCGAAGAGGACATTAAAGCGTTTTGTAAGAAGAATTTGGCAGCTTTCAAGGTGCCAAAGAGAGTGTTCATCACTGATAACCTCCCCAAAACTGCCTCTGGTAAGATTCAGCGCCGTATCGTCGCACAACATTTCCTTGAGAAGCCC
SEQ ID NO:101(核糖体结合位点RBS6和At_AAE3开放读码框):
GCATCATCTACCTCAATTATTCACCATTCCATCTGTCTAGCCAAAAAATTTAAAAAAATGGATAGCGATACTCTCTCAGGATTATTGGAAAACGTCGCCAAAAAATTCCCCGATCGCCGAGCTCTCTCCGTTTCTGGAAAATTCAATCTCACTCACGCGCGTCTTCACGATCTAATCGAACGCGCCGCTTCACGCCTTGTCTCCGACGCTGGAATCAAACCCGGCGATGTCGTTGCTCTCACCTTCCCTAACACCGTCGAGTTTGTTATAATGTTTTTAGCGGTGATAAGAGCTAGAGCCACGGCGGCGCCGTTGAACGCAGCGTACACGGCGGAGGAATTTGAGTTTTACCTCTCCGATTCAGATTCAAAGCTATTGTTAACCTCTAAAGAAGGAAACGCACCGGCTCAAGAAGCAGCTTCAAAGCTGAAAATCTCTCACGTCACCGCTACGCTGCTTGACGCTGGCTCGGACCTTGTACTATCCGTTGCGGATTCAGATTCCGTCGTTGACTCAGCGACGGAACTCGTTAATCACCCGGACGACGGTGCTCTCTTCCTCCACACTTCTGGCACTACGAGCCGTCCAAAGGGTGTACCGCTTACGCAGCTCAATCTAGCTTCATCCGTCAAGAACATTAAAGCTGTGTACAAGCTTACTGAGTCTGATTCTACGGTGATTGTTCTCCCTCTGTTCCATGTTCATGGATTGTTAGCTGGGTTGCTTAGCTCGCTTGGAGCTGGTGCTGCTGTAACTCTTCCAGCTGCTGGTAGATTCTCAGCAACAACATTTTGGCCAGATATGAAGAAGTATAACGCTACATGGTATACTGCTGTGCCGACCATTCATCAGATCATATTGGACCGCCACGCGAGCCACCCTGAAACGGAATATCCTAAACTCCGGTTCATCAGGAGTTGCAGTGCTTCTTTGGCTCCGGTGATATTGTCCAGGCTTGAGGAAGCGTTTGGAGCACCGGTGCTCGAGGCCTATGCAATGACAGAGGCAACACATTTGATGAGCTCAAACCCCTTACCAGAGGAAGGTCCACACAAGCCTGGGTCTGTTGGGAAACCGGTAGGTCAAGAAATGGCGATCCTTAATGAGAAAGGCGAGATCCAAGAGCCAAATAACAAAGGAGAGGTTTGTATAAGAGGTCCAAATGTGACCAAGGGTTACAAGAATAACCCAGAAGCCAACAAGGCAGGTTTCGAGTTTGGGTGGTTCCACACTGGTGATATCGGTTACTTTGATACCGATGGGTATTTGCATCTGGTGGGTCGGATCAAAGAGCTTATTAACCGTGGAGGTGAGAAGATATCTCCAATTGAAGTGGATGCAGTACTCTTAACGCATCCTGACGTTTCTCAGGGTGTTGCATTCGGTGTTCCTGATGAGAAATATGGGGAAGAGATTAACTGTGCGGTGATTCCAAGAGAAGGAACTACTGTAACCGAAGAGGACATTAAAGCGTTTTGTAAGAAGAATTTGGCAGCTTTCAAGGTGCCAAAGAGAGTGTTCATCACTGATAACCTCCCCAAAACTGCCTCTGGTAAGATTCAGCGCCGTATCGTCGCACAACATTTCCTTGAGAAGCCC
SEQ ID NO:102(核糖体结合位点RBS7和At_AAE3开放读码框):
GCATCATCTACCTAAATTACTCATCATCCCATTTTAGTCAGCTTAAAATATTATAAAATGGATAGCGATACTCTCTCAGGATTATTGGAAAACGTCGCCAAAAAATTCCCCGATCGCCGAGCTCTCTCCGTTTCTGGAAAATTCAATCTCACTCACGCGCGTCTTCACGATCTAATCGAACGCGCCGCTTCACGCCTTGTCTCCGACGCTGGAATCAAACCCGGCGATGTCGTTGCTCTCACCTTCCCTAACACCGTCGAGTTTGTTATAATGTTTTTAGCGGTGATAAGAGCTAGAGCCACGGCGGCGCCGTTGAACGCAGCGTACACGGCGGAGGAATTTGAGTTTTACCTCTCCGATTCAGATTCAAAGCTATTGTTAACCTCTAAAGAAGGAAACGCACCGGCTCAAGAAGCAGCTTCAAAGCTGAAAATCTCTCACGTCACCGCTACGCTGCTTGACGCTGGCTCGGACCTTGTACTATCCGTTGCGGATTCAGATTCCGTCGTTGACTCAGCGACGGAACTCGTTAATCACCCGGACGACGGTGCTCTCTTCCTCCACACTTCTGGCACTACGAGCCGTCCAAAGGGTGTACCGCTTACGCAGCTCAATCTAGCTTCATCCGTCAAGAACATTAAAGCTGTGTACAAGCTTACTGAGTCTGATTCTACGGTGATTGTTCTCCCTCTGTTCCATGTTCATGGATTGTTAGCTGGGTTGCTTAGCTCGCTTGGAGCTGGTGCTGCTGTAACTCTTCCAGCTGCTGGTAGATTCTCAGCAACAACATTTTGGCCAGATATGAAGAAGTATAACGCTACATGGTATACTGCTGTGCCGACCATTCATCAGATCATATTGGACCGCCACGCGAGCCACCCTGAAACGGAATATCCTAAACTCCGGTTCATCAGGAGTTGCAGTGCTTCTTTGGCTCCGGTGATATTGTCCAGGCTTGAGGAAGCGTTTGGAGCACCGGTGCTCGAGGCCTATGCAATGACAGAGGCAACACATTTGATGAGCTCAAACCCCTTACCAGAGGAAGGTCCACACAAGCCTGGGTCTGTTGGGAAACCGGTAGGTCAAGAAATGGCGATCCTTAATGAGAAAGGCGAGATCCAAGAGCCAAATAACAAAGGAGAGGTTTGTATAAGAGGTCCAAATGTGACCAAGGGTTACAAGAATAACCCAGAAGCCAACAAGGCAGGTTTCGAGTTTGGGTGGTTCCACACTGGTGATATCGGTTACTTTGATACCGATGGGTATTTGCATCTGGTGGGTCGGATCAAAGAGCTTATTAACCGTGGAGGTGAGAAGATATCTCCAATTGAAGTGGATGCAGTACTCTTAACGCATCCTGACGTTTCTCAGGGTGTTGCATTCGGTGTTCCTGATGAGAAATATGGGGAAGAGATTAACTGTGCGGTGATTCCAAGAGAAGGAACTACTGTAACCGAAGAGGACATTAAAGCGTTTTGTAAGAAGAATTTGGCAGCTTTCAAGGTGCCAAAGAGAGTGTTCATCACTGATAACCTCCCCAAAACTGCCTCTGGTAAGATTCAGCGCCGTATCGTCGCACAACATTTCCTTGAGAAGCCC
SEQ ID NO:103(核糖体结合位点RBS8和At_AAE3开放读码框):
GCATCATCTACCTATATTATTTATCATTCCATATCATGCGTGAATAAAAATAATAAAATGGATAGCGATACTCTCTCAGGATTATTGGAAAACGTCGCCAAAAAATTCCCCGATCGCCGAGCTCTCTCCGTTTCTGGAAAATTCAATCTCACTCACGCGCGTCTTCACGATCTAATCGAACGCGCCGCTTCACGCCTTGTCTCCGACGCTGGAATCAAACCCGGCGATGTCGTTGCTCTCACCTTCCCTAACACCGTCGAGTTTGTTATAATGTTTTTAGCGGTGATAAGAGCTAGAGCCACGGCGGCGCCGTTGAACGCAGCGTACACGGCGGAGGAATTTGAGTTTTACCTCTCCGATTCAGATTCAAAGCTATTGTTAACCTCTAAAGAAGGAAACGCACCGGCTCAAGAAGCAGCTTCAAAGCTGAAAATCTCTCACGTCACCGCTACGCTGCTTGACGCTGGCTCGGACCTTGTACTATCCGTTGCGGATTCAGATTCCGTCGTTGACTCAGCGACGGAACTCGTTAATCACCCGGACGACGGTGCTCTCTTCCTCCACACTTCTGGCACTACGAGCCGTCCAAAGGGTGTACCGCTTACGCAGCTCAATCTAGCTTCATCCGTCAAGAACATTAAAGCTGTGTACAAGCTTACTGAGTCTGATTCTACGGTGATTGTTCTCCCTCTGTTCCATGTTCATGGATTGTTAGCTGGGTTGCTTAGCTCGCTTGGAGCTGGTGCTGCTGTAACTCTTCCAGCTGCTGGTAGATTCTCAGCAACAACATTTTGGCCAGATATGAAGAAGTATAACGCTACATGGTATACTGCTGTGCCGACCATTCATCAGATCATATTGGACCGCCACGCGAGCCACCCTGAAACGGAATATCCTAAACTCCGGTTCATCAGGAGTTGCAGTGCTTCTTTGGCTCCGGTGATATTGTCCAGGCTTGAGGAAGCGTTTGGAGCACCGGTGCTCGAGGCCTATGCAATGACAGAGGCAACACATTTGATGAGCTCAAACCCCTTACCAGAGGAAGGTCCACACAAGCCTGGGTCTGTTGGGAAACCGGTAGGTCAAGAAATGGCGATCCTTAATGAGAAAGGCGAGATCCAAGAGCCAAATAACAAAGGAGAGGTTTGTATAAGAGGTCCAAATGTGACCAAGGGTTACAAGAATAACCCAGAAGCCAACAAGGCAGGTTTCGAGTTTGGGTGGTTCCACACTGGTGATATCGGTTACTTTGATACCGATGGGTATTTGCATCTGGTGGGTCGGATCAAAGAGCTTATTAACCGTGGAGGTGAGAAGATATCTCCAATTGAAGTGGATGCAGTACTCTTAACGCATCCTGACGTTTCTCAGGGTGTTGCATTCGGTGTTCCTGATGAGAAATATGGGGAAGAGATTAACTGTGCGGTGATTCCAAGAGAAGGAACTACTGTAACCGAAGAGGACATTAAAGCGTTTTGTAAGAAGAATTTGGCAGCTTTCAAGGTGCCAAAGAGAGTGTTCATCACTGATAACCTCCCCAAAACTGCCTCTGGTAAGATTCAGCGCCGTATCGTCGCACAACATTTCCTTGAGAAGCCC
SEQ ID NO:104(核糖体结合位点RBS1和Sc_YBR222C开放读码框):
GCATCATCTACCTATATTAATCATCAACCCAATTTTCATATGTATAAAATTAACAAAATGACAAGTGCCGCTACTGTTACTGCTTCGTTCAACGATACTTTTAGCGTATCCGATAATGTCGCCGTTATTGTTCCTGAAACGGACACTCAGGTGACCTACAGGGATCTATCCCACATGGTGGGTCACTTCCAGACCATGTTCACAAATCCTAATTCTCCATTGTACGGAGCTGTTTTCAGACAAGATACAGTGGCGATATCCATGCGTAATGGGCTGGAATTTATCGTCGCTTTCCTCGGTGCTACTATGGACGCTAAAATTGGCGCGCCCTTGAATCCCAATTATAAGGAAAAGGAGTTCAATTTTTATTTGAATGACCTGAAATCTAAGGCGATTTGCGTCCCAAAGGGTACCACAAAGTTACAGAGTTCTGAAATTCTAAAATCTGCCTCCACGTTTGGATGTTTTATCGTAGAGCTGGCCTTCGATGCGACCAGGTTTAGGGTAGAGTATGATATATACTCTCCAGAGGACAACTACAAAAGGGTTATTTACCGGTCTTTGAACAACGCCAAATTTGTCAACACAAATCCCGTTAAATTCCCTGGGTTTGCCCGTTCCAGTGACGTTGCCCTGATTTTGCATACCAGTGGTACCACCTCCACTCCAAAAACGGTGCCTTTGTTACATTTGAACATTGTGAGAAGCACGTTGAACATTGCTAACACTTACAAGCTAACGCCCTTGGACAGATCTTATGTCGTGATGCCTCTTTTCCACGTCCATGGGTTAATTGGTGTTTTACTTTCCACTTTTAGAACTCAGGGTTCTGTTGTGGTTCCCGATGGATTCCATCCAAAGTTATTCTGGGACCAATTTGTTAAGTACAACTGTAATTGGTTCAGTTGCGTTCCCACAATAAGCATGATTATGCTGAACATGCCCAAACCAAACCCTTTCCCACACATTAGATTCATCAGATCGTGTTCTTCTGCTTTGGCTCCAGCAACGTTCCATAAGCTGGAGAAGGAATTCAATGCACCTGTCTTGGAGGCCTATGCGATGACCGAAGCATCACATCAAATGACCTCAAACAATCTGCCTCCAGGAAAGAGAAAGCCTGGTACTGTGGGCCAGCCACAAGGAGTCACCGTCGTCATTCTAGATGACAATGACAATGTCTTGCCCCCGGGCAAAGTCGGCGAAGTTTCCATCAGAGGCGAAAACGTCACTTTGGGGTATGCTAATAATCCAAAAGCTAACAAGGAGAACTTCACCAAGAGAGAGAACTATTTCAGAACCGGTGACCAAGGTTATTTCGACCCTGAGGGGTTTTTGGTCCTTACAGGCAGAATCAAAGAGCTTATCAACAGGGGTGGTGAAAAGATTTCACCCATTGAGCTCGACGGCATTATGCTATCGCATCCAAAGATCGATGAAGCCGTTGCATTTGGTGTTCCCGACGATATGTACGGCCAAGTAGTTCAAGCCGCCATTGTTTTGAAGAAGGGAGAAAAAATGACCTACGAAGAACTGGTGAACTTCTTAAAGAAGCACCTAGCCTCTTTCAAAATTCCAACCAAGGTGTACTTTGTTGATAAGCTACCAAAAACCGCTACAGGTAAAATCCAGAGAAGAGTTATCGCAGAAACTTTTGCTAAGAGCAGCAGAAATAAGAGTAAGTTGSEQ ID NO:105(核糖体结合位点RBS2和Sc_YBR222C开放读码框):
GCATCTTTTCCATAACAAAAATTAAACCTCCTCCATATGAAAAATAAAAATTATAAAATGACAAGTGCCGCTACTGTTACTGCTTCGTTCAACGATACTTTTAGCGTATCCGATAATGTCGCCGTTATTGTTCCTGAAACGGACACTCAGGTGACCTACAGGGATCTATCCCACATGGTGGGTCACTTCCAGACCATGTTCACAAATCCTAATTCTCCATTGTACGGAGCTGTTTTCAGACAAGATACAGTGGCGATATCCATGCGTAATGGGCTGGAATTTATCGTCGCTTTCCTCGGTGCTACTATGGACGCTAAAATTGGCGCGCCCTTGAATCCCAATTATAAGGAAAAGGAGTTCAATTTTTATTTGAATGACCTGAAATCTAAGGCGATTTGCGTCCCAAAGGGTACCACAAAGTTACAGAGTTCTGAAATTCTAAAATCTGCCTCCACGTTTGGATGTTTTATCGTAGAGCTGGCCTTCGATGCGACCAGGTTTAGGGTAGAGTATGATATATACTCTCCAGAGGACAACTACAAAAGGGTTATTTACCGGTCTTTGAACAACGCCAAATTTGTCAACACAAATCCCGTTAAATTCCCTGGGTTTGCCCGTTCCAGTGACGTTGCCCTGATTTTGCATACCAGTGGTACCACCTCCACTCCAAAAACGGTGCCTTTGTTACATTTGAACATTGTGAGAAGCACGTTGAACATTGCTAACACTTACAAGCTAACGCCCTTGGACAGATCTTATGTCGTGATGCCTCTTTTCCACGTCCATGGGTTAATTGGTGTTTTACTTTCCACTTTTAGAACTCAGGGTTCTGTTGTGGTTCCCGATGGATTCCATCCAAAGTTATTCTGGGACCAATTTGTTAAGTACAACTGTAATTGGTTCAGTTGCGTTCCCACAATAAGCATGATTATGCTGAACATGCCCAAACCAAACCCTTTCCCACACATTAGATTCATCAGATCGTGTTCTTCTGCTTTGGCTCCAGCAACGTTCCATAAGCTGGAGAAGGAATTCAATGCACCTGTCTTGGAGGCCTATGCGATGACCGAAGCATCACATCAAATGACCTCAAACAATCTGCCTCCAGGAAAGAGAAAGCCTGGTACTGTGGGCCAGCCACAAGGAGTCACCGTCGTCATTCTAGATGACAATGACAATGTCTTGCCCCCGGGCAAAGTCGGCGAAGTTTCCATCAGAGGCGAAAACGTCACTTTGGGGTATGCTAATAATCCAAAAGCTAACAAGGAGAACTTCACCAAGAGAGAGAACTATTTCAGAACCGGTGACCAAGGTTATTTCGACCCTGAGGGGTTTTTGGTCCTTACAGGCAGAATCAAAGAGCTTATCAACAGGGGTGGTGAAAAGATTTCACCCATTGAGCTCGACGGCATTATGCTATCGCATCCAAAGATCGATGAAGCCGTTGCATTTGGTGTTCCCGACGATATGTACGGCCAAGTAGTTCAAGCCGCCATTGTTTTGAAGAAGGGAGAAAAAATGACCTACGAAGAACTGGTGAACTTCTTAAAGAAGCACCTAGCCTCTTTCAAAATTCCAACCAAGGTGTACTTTGTTGATAAGCTACCAAAAACCGCTACAGGTAAAATCCAGAGAAGAGTTATCGCAGAAACTTTTGCTAAGAGCAGCAGAAATAAGAGTAAGTTGSEQ ID NO:106(核糖体结合位点RBS3和Sc_YBR222C开放读码框):
GCATCAACTACCTCAATTAATAATCAAACCATCTGAACAATGAATAAATTTAAAAAAATGACAAGTGCCGCTACTGTTACTGCTTCGTTCAACGATACTTTTAGCGTATCCGATAATGTCGCCGTTATTGTTCCTGAAACGGACACTCAGGTGACCTACAGGGATCTATCCCACATGGTGGGTCACTTCCAGACCATGTTCACAAATCCTAATTCTCCATTGTACGGAGCTGTTTTCAGACAAGATACAGTGGCGATATCCATGCGTAATGGGCTGGAATTTATCGTCGCTTTCCTCGGTGCTACTATGGACGCTAAAATTGGCGCGCCCTTGAATCCCAATTATAAGGAAAAGGAGTTCAATTTTTATTTGAATGACCTGAAATCTAAGGCGATTTGCGTCCCAAAGGGTACCACAAAGTTACAGAGTTCTGAAATTCTAAAATCTGCCTCCACGTTTGGATGTTTTATCGTAGAGCTGGCCTTCGATGCGACCAGGTTTAGGGTAGAGTATGATATATACTCTCCAGAGGACAACTACAAAAGGGTTATTTACCGGTCTTTGAACAACGCCAAATTTGTCAACACAAATCCCGTTAAATTCCCTGGGTTTGCCCGTTCCAGTGACGTTGCCCTGATTTTGCATACCAGTGGTACCACCTCCACTCCAAAAACGGTGCCTTTGTTACATTTGAACATTGTGAGAAGCACGTTGAACATTGCTAACACTTACAAGCTAACGCCCTTGGACAGATCTTATGTCGTGATGCCTCTTTTCCACGTCCATGGGTTAATTGGTGTTTTACTTTCCACTTTTAGAACTCAGGGTTCTGTTGTGGTTCCCGATGGATTCCATCCAAAGTTATTCTGGGACCAATTTGTTAAGTACAACTGTAATTGGTTCAGTTGCGTTCCCACAATAAGCATGATTATGCTGAACATGCCCAAACCAAACCCTTTCCCACACATTAGATTCATCAGATCGTGTTCTTCTGCTTTGGCTCCAGCAACGTTCCATAAGCTGGAGAAGGAATTCAATGCACCTGTCTTGGAGGCCTATGCGATGACCGAAGCATCACATCAAATGACCTCAAACAATCTGCCTCCAGGAAAGAGAAAGCCTGGTACTGTGGGCCAGCCACAAGGAGTCACCGTCGTCATTCTAGATGACAATGACAATGTCTTGCCCCCGGGCAAAGTCGGCGAAGTTTCCATCAGAGGCGAAAACGTCACTTTGGGGTATGCTAATAATCCAAAAGCTAACAAGGAGAACTTCACCAAGAGAGAGAACTATTTCAGAACCGGTGACCAAGGTTATTTCGACCCTGAGGGGTTTTTGGTCCTTACAGGCAGAATCAAAGAGCTTATCAACAGGGGTGGTGAAAAGATTTCACCCATTGAGCTCGACGGCATTATGCTATCGCATCCAAAGATCGATGAAGCCGTTGCATTTGGTGTTCCCGACGATATGTACGGCCAAGTAGTTCAAGCCGCCATTGTTTTGAAGAAGGGAGAAAAAATGACCTACGAAGAACTGGTGAACTTCTTAAAGAAGCACCTAGCCTCTTTCAAAATTCCAACCAAGGTGTACTTTGTTGATAAGCTACCAAAAACCGCTACAGGTAAAATCCAGAGAAGAGTTATCGCAGAAACTTTTGCTAAGAGCAGCAGAAATAAGAGTAAGTTG
SEQ ID NO:107(核糖体结合位点RBS4和Sc_YBR222C开放读码框):
GCATCTTTTCCATAACAAAAATTAAACCTCCTCCATATGAGATATAAAATTAACAAAATGACAAGTGCCGCTACTGTTACTGCTTCGTTCAACGATACTTTTAGCGTATCCGATAATGTCGCCGTTATTGTTCCTGAAACGGACACTCAGGTGACCTACAGGGATCTATCCCACATGGTGGGTCACTTCCAGACCATGTTCACAAATCCTAATTCTCCATTGTACGGAGCTGTTTTCAGACAAGATACAGTGGCGATATCCATGCGTAATGGGCTGGAATTTATCGTCGCTTTCCTCGGTGCTACTATGGACGCTAAAATTGGCGCGCCCTTGAATCCCAATTATAAGGAAAAGGAGTTCAATTTTTATTTGAATGACCTGAAATCTAAGGCGATTTGCGTCCCAAAGGGTACCACAAAGTTACAGAGTTCTGAAATTCTAAAATCTGCCTCCACGTTTGGATGTTTTATCGTAGAGCTGGCCTTCGATGCGACCAGGTTTAGGGTAGAGTATGATATATACTCTCCAGAGGACAACTACAAAAGGGTTATTTACCGGTCTTTGAACAACGCCAAATTTGTCAACACAAATCCCGTTAAATTCCCTGGGTTTGCCCGTTCCAGTGACGTTGCCCTGATTTTGCATACCAGTGGTACCACCTCCACTCCAAAAACGGTGCCTTTGTTACATTTGAACATTGTGAGAAGCACGTTGAACATTGCTAACACTTACAAGCTAACGCCCTTGGACAGATCTTATGTCGTGATGCCTCTTTTCCACGTCCATGGGTTAATTGGTGTTTTACTTTCCACTTTTAGAACTCAGGGTTCTGTTGTGGTTCCCGATGGATTCCATCCAAAGTTATTCTGGGACCAATTTGTTAAGTACAACTGTAATTGGTTCAGTTGCGTTCCCACAATAAGCATGATTATGCTGAACATGCCCAAACCAAACCCTTTCCCACACATTAGATTCATCAGATCGTGTTCTTCTGCTTTGGCTCCAGCAACGTTCCATAAGCTGGAGAAGGAATTCAATGCACCTGTCTTGGAGGCCTATGCGATGACCGAAGCATCACATCAAATGACCTCAAACAATCTGCCTCCAGGAAAGAGAAAGCCTGGTACTGTGGGCCAGCCACAAGGAGTCACCGTCGTCATTCTAGATGACAATGACAATGTCTTGCCCCCGGGCAAAGTCGGCGAAGTTTCCATCAGAGGCGAAAACGTCACTTTGGGGTATGCTAATAATCCAAAAGCTAACAAGGAGAACTTCACCAAGAGAGAGAACTATTTCAGAACCGGTGACCAAGGTTATTTCGACCCTGAGGGGTTTTTGGTCCTTACAGGCAGAATCAAAGAGCTTATCAACAGGGGTGGTGAAAAGATTTCACCCATTGAGCTCGACGGCATTATGCTATCGCATCCAAAGATCGATGAAGCCGTTGCATTTGGTGTTCCCGACGATATGTACGGCCAAGTAGTTCAAGCCGCCATTGTTTTGAAGAAGGGAGAAAAAATGACCTACGAAGAACTGGTGAACTTCTTAAAGAAGCACCTAGCCTCTTTCAAAATTCCAACCAAGGTGTACTTTGTTGATAAGCTACCAAAAACCGCTACAGGTAAAATCCAGAGAAGAGTTATCGCAGAAACTTTTGCTAAGAGCAGCAGAAATAAGAGTAAGTTG
SEQ ID NO:108(核糖体结合位点RBS5和Sc_YBR222C开放读码框):
GCATCAACTACCTTAATTATTCATCAAACCATATAACATACTAAAAAAAATAACAAAATGACAAGTGCCGCTACTGTTACTGCTTCGTTCAACGATACTTTTAGCGTATCCGATAATGTCGCCGTTATTGTTCCTGAAACGGACACTCAGGTGACCTACAGGGATCTATCCCACATGGTGGGTCACTTCCAGACCATGTTCACAAATCCTAATTCTCCATTGTACGGAGCTGTTTTCAGACAAGATACAGTGGCGATATCCATGCGTAATGGGCTGGAATTTATCGTCGCTTTCCTCGGTGCTACTATGGACGCTAAAATTGGCGCGCCCTTGAATCCCAATTATAAGGAAAAGGAGTTCAATTTTTATTTGAATGACCTGAAATCTAAGGCGATTTGCGTCCCAAAGGGTACCACAAAGTTACAGAGTTCTGAAATTCTAAAATCTGCCTCCACGTTTGGATGTTTTATCGTAGAGCTGGCCTTCGATGCGACCAGGTTTAGGGTAGAGTATGATATATACTCTCCAGAGGACAACTACAAAAGGGTTATTTACCGGTCTTTGAACAACGCCAAATTTGTCAACACAAATCCCGTTAAATTCCCTGGGTTTGCCCGTTCCAGTGACGTTGCCCTGATTTTGCATACCAGTGGTACCACCTCCACTCCAAAAACGGTGCCTTTGTTACATTTGAACATTGTGAGAAGCACGTTGAACATTGCTAACACTTACAAGCTAACGCCCTTGGACAGATCTTATGTCGTGATGCCTCTTTTCCACGTCCATGGGTTAATTGGTGTTTTACTTTCCACTTTTAGAACTCAGGGTTCTGTTGTGGTTCCCGATGGATTCCATCCAAAGTTATTCTGGGACCAATTTGTTAAGTACAACTGTAATTGGTTCAGTTGCGTTCCCACAATAAGCATGATTATGCTGAACATGCCCAAACCAAACCCTTTCCCACACATTAGATTCATCAGATCGTGTTCTTCTGCTTTGGCTCCAGCAACGTTCCATAAGCTGGAGAAGGAATTCAATGCACCTGTCTTGGAGGCCTATGCGATGACCGAAGCATCACATCAAATGACCTCAAACAATCTGCCTCCAGGAAAGAGAAAGCCTGGTACTGTGGGCCAGCCACAAGGAGTCACCGTCGTCATTCTAGATGACAATGACAATGTCTTGCCCCCGGGCAAAGTCGGCGAAGTTTCCATCAGAGGCGAAAACGTCACTTTGGGGTATGCTAATAATCCAAAAGCTAACAAGGAGAACTTCACCAAGAGAGAGAACTATTTCAGAACCGGTGACCAAGGTTATTTCGACCCTGAGGGGTTTTTGGTCCTTACAGGCAGAATCAAAGAGCTTATCAACAGGGGTGGTGAAAAGATTTCACCCATTGAGCTCGACGGCATTATGCTATCGCATCCAAAGATCGATGAAGCCGTTGCATTTGGTGTTCCCGACGATATGTACGGCCAAGTAGTTCAAGCCGCCATTGTTTTGAAGAAGGGAGAAAAAATGACCTACGAAGAACTGGTGAACTTCTTAAAGAAGCACCTAGCCTCTTTCAAAATTCCAACCAAGGTGTACTTTGTTGATAAGCTACCAAAAACCGCTACAGGTAAAATCCAGAGAAGAGTTATCGCAGAAACTTTTGCTAAGAGCAGCAGAAATAAGAGTAAGTTG
SEQ ID NO:109(核糖体结合位点RBS6和Sc_YBR222C开放读码框):
GCATCATCTACCTATATTACTAATCATTCCAATTAATTACCTTATAAAATTAAGAAAATGACAAGTGCCGCTACTGTTACTGCTTCGTTCAACGATACTTTTAGCGTATCCGATAATGTCGCCGTTATTGTTCCTGAAACGGACACTCAGGTGACCTACAGGGATCTATCCCACATGGTGGGTCACTTCCAGACCATGTTCACAAATCCTAATTCTCCATTGTACGGAGCTGTTTTCAGACAAGATACAGTGGCGATATCCATGCGTAATGGGCTGGAATTTATCGTCGCTTTCCTCGGTGCTACTATGGACGCTAAAATTGGCGCGCCCTTGAATCCCAATTATAAGGAAAAGGAGTTCAATTTTTATTTGAATGACCTGAAATCTAAGGCGATTTGCGTCCCAAAGGGTACCACAAAGTTACAGAGTTCTGAAATTCTAAAATCTGCCTCCACGTTTGGATGTTTTATCGTAGAGCTGGCCTTCGATGCGACCAGGTTTAGGGTAGAGTATGATATATACTCTCCAGAGGACAACTACAAAAGGGTTATTTACCGGTCTTTGAACAACGCCAAATTTGTCAACACAAATCCCGTTAAATTCCCTGGGTTTGCCCGTTCCAGTGACGTTGCCCTGATTTTGCATACCAGTGGTACCACCTCCACTCCAAAAACGGTGCCTTTGTTACATTTGAACATTGTGAGAAGCACGTTGAACATTGCTAACACTTACAAGCTAACGCCCTTGGACAGATCTTATGTCGTGATGCCTCTTTTCCACGTCCATGGGTTAATTGGTGTTTTACTTTCCACTTTTAGAACTCAGGGTTCTGTTGTGGTTCCCGATGGATTCCATCCAAAGTTATTCTGGGACCAATTTGTTAAGTACAACTGTAATTGGTTCAGTTGCGTTCCCACAATAAGCATGATTATGCTGAACATGCCCAAACCAAACCCTTTCCCACACATTAGATTCATCAGATCGTGTTCTTCTGCTTTGGCTCCAGCAACGTTCCATAAGCTGGAGAAGGAATTCAATGCACCTGTCTTGGAGGCCTATGCGATGACCGAAGCATCACATCAAATGACCTCAAACAATCTGCCTCCAGGAAAGAGAAAGCCTGGTACTGTGGGCCAGCCACAAGGAGTCACCGTCGTCATTCTAGATGACAATGACAATGTCTTGCCCCCGGGCAAAGTCGGCGAAGTTTCCATCAGAGGCGAAAACGTCACTTTGGGGTATGCTAATAATCCAAAAGCTAACAAGGAGAACTTCACCAAGAGAGAGAACTATTTCAGAACCGGTGACCAAGGTTATTTCGACCCTGAGGGGTTTTTGGTCCTTACAGGCAGAATCAAAGAGCTTATCAACAGGGGTGGTGAAAAGATTTCACCCATTGAGCTCGACGGCATTATGCTATCGCATCCAAAGATCGATGAAGCCGTTGCATTTGGTGTTCCCGACGATATGTACGGCCAAGTAGTTCAAGCCGCCATTGTTTTGAAGAAGGGAGAAAAAATGACCTACGAAGAACTGGTGAACTTCTTAAAGAAGCACCTAGCCTCTTTCAAAATTCCAACCAAGGTGTACTTTGTTGATAAGCTACCAAAAACCGCTACAGGTAAAATCCAGAGAAGAGTTATCGCAGAAACTTTTGCTAAGAGCAGCAGAAATAAGAGTAAGTTG
SEQ ID NO:110(核糖体结合位点RBS7和Sc_YBR222C开放读码框):
GCATCTTTTCCATAACAAAAATTAAACCTCCTCCCCCCCACGTTTAAAATTAAAAAAATGACAAGTGCCGCTACTGTTACTGCTTCGTTCAACGATACTTTTAGCGTATCCGATAATGTCGCCGTTATTGTTCCTGAAACGGACACTCAGGTGACCTACAGGGATCTATCCCACATGGTGGGTCACTTCCAGACCATGTTCACAAATCCTAATTCTCCATTGTACGGAGCTGTTTTCAGACAAGATACAGTGGCGATATCCATGCGTAATGGGCTGGAATTTATCGTCGCTTTCCTCGGTGCTACTATGGACGCTAAAATTGGCGCGCCCTTGAATCCCAATTATAAGGAAAAGGAGTTCAATTTTTATTTGAATGACCTGAAATCTAAGGCGATTTGCGTCCCAAAGGGTACCACAAAGTTACAGAGTTCTGAAATTCTAAAATCTGCCTCCACGTTTGGATGTTTTATCGTAGAGCTGGCCTTCGATGCGACCAGGTTTAGGGTAGAGTATGATATATACTCTCCAGAGGACAACTACAAAAGGGTTATTTACCGGTCTTTGAACAACGCCAAATTTGTCAACACAAATCCCGTTAAATTCCCTGGGTTTGCCCGTTCCAGTGACGTTGCCCTGATTTTGCATACCAGTGGTACCACCTCCACTCCAAAAACGGTGCCTTTGTTACATTTGAACATTGTGAGAAGCACGTTGAACATTGCTAACACTTACAAGCTAACGCCCTTGGACAGATCTTATGTCGTGATGCCTCTTTTCCACGTCCATGGGTTAATTGGTGTTTTACTTTCCACTTTTAGAACTCAGGGTTCTGTTGTGGTTCCCGATGGATTCCATCCAAAGTTATTCTGGGACCAATTTGTTAAGTACAACTGTAATTGGTTCAGTTGCGTTCCCACAATAAGCATGATTATGCTGAACATGCCCAAACCAAACCCTTTCCCACACATTAGATTCATCAGATCGTGTTCTTCTGCTTTGGCTCCAGCAACGTTCCATAAGCTGGAGAAGGAATTCAATGCACCTGTCTTGGAGGCCTATGCGATGACCGAAGCATCACATCAAATGACCTCAAACAATCTGCCTCCAGGAAAGAGAAAGCCTGGTACTGTGGGCCAGCCACAAGGAGTCACCGTCGTCATTCTAGATGACAATGACAATGTCTTGCCCCCGGGCAAAGTCGGCGAAGTTTCCATCAGAGGCGAAAACGTCACTTTGGGGTATGCTAATAATCCAAAAGCTAACAAGGAGAACTTCACCAAGAGAGAGAACTATTTCAGAACCGGTGACCAAGGTTATTTCGACCCTGAGGGGTTTTTGGTCCTTACAGGCAGAATCAAAGAGCTTATCAACAGGGGTGGTGAAAAGATTTCACCCATTGAGCTCGACGGCATTATGCTATCGCATCCAAAGATCGATGAAGCCGTTGCATTTGGTGTTCCCGACGATATGTACGGCCAAGTAGTTCAAGCCGCCATTGTTTTGAAGAAGGGAGAAAAAATGACCTACGAAGAACTGGTGAACTTCTTAAAGAAGCACCTAGCCTCTTTCAAAATTCCAACCAAGGTGTACTTTGTTGATAAGCTACCAAAAACCGCTACAGGTAAAATCCAGAGAAGAGTTATCGCAGAAACTTTTGCTAAGAGCAGCAGAAATAAGAGTAAGTTG
SEQ ID NO:111(核糖体结合位点RBS8和Sc_YBR222C开放读码框)：
GCATCAACTACCTTCATTACTTAACAATCCACCTGTTGTGAGTAAAAATATAAGAAAATGACAAGTGCCGCTACTGTTACTGCTTCGTTCAACGATACTTTTAGCGTATCCGATAATGTCGCCGTTATTGTTCCTGAAACGGACACTCAGGTGACCTACAGGGATCTATCCCACATGGTGGGTCACTTCCAGACCATGTTCACAAATCCTAATTCTCCATTGTACGGAGCTGTTTTCAGACAAGATACAGTGGCGATATCCATGCGTAATGGGCTGGAATTTATCGTCGCTTTCCTCGGTGCTACTATGGACGCTAAAATTGGCGCGCCCTTGAATCCCAATTATAAGGAAAAGGAGTTCAATTTTTATTTGAATGACCTGAAATCTAAGGCGATTTGCGTCCCAAAGGGTACCACAAAGTTACAGAGTTCTGAAATTCTAAAATCTGCCTCCACGTTTGGATGTTTTATCGTAGAGCTGGCCTTCGATGCGACCAGGTTTAGGGTAGAGTATGATATATACTCTCCAGAGGACAACTACAAAAGGGTTATTTACCGGTCTTTGAACAACGCCAAATTTGTCAACACAAATCCCGTTAAATTCCCTGGGTTTGCCCGTTCCAGTGACGTTGCCCTGATTTTGCATACCAGTGGTACCACCTCCACTCCAAAAACGGTGCCTTTGTTACATTTGAACATTGTGAGAAGCACGTTGAACATTGCTAACACTTACAAGCTAACGCCCTTGGACAGATCTTATGTCGTGATGCCTCTTTTCCACGTCCATGGGTTAATTGGTGTTTTACTTTCCACTTTTAGAACTCAGGGTTCTGTTGTGGTTCCCGATGGATTCCATCCAAAGTTATTCTGGGACCAATTTGTTAAGTACAACTGTAATTGGTTCAGTTGCGTTCCCACAATAAGCATGATTATGCTGAACATGCCCAAACCAAACCCTTTCCCACACATTAGATTCATCAGATCGTGTTCTTCTGCTTTGGCTCCAGCAACGTTCCATAAGCTGGAGAAGGAATTCAATGCACCTGTCTTGGAGGCCTATGCGATGACCGAAGCATCACATCAAATGACCTCAAACAATCTGCCTCCAGGAAAGAGAAAGCCTGGTACTGTGGGCCAGCCACAAGGAGTCACCGTCGTCATTCTAGATGACAATGACAATGTCTTGCCCCCGGGCAAAGTCGGCGAAGTTTCCATCAGAGGCGAAAACGTCACTTTGGGGTATGCTAATAATCCAAAAGCTAACAAGGAGAACTTCACCAAGAGAGAGAACTATTTCAGAACCGGTGACCAAGGTTATTTCGACCCTGAGGGGTTTTTGGTCCTTACAGGCAGAATCAAAGAGCTTATCAACAGGGGTGGTGAAAAGATTTCACCCATTGAGCTCGACGGCATTATGCTATCGCATCCAAAGATCGATGAAGCCGTTGCATTTGGTGTTCCCGACGATATGTACGGCCAAGTAGTTCAAGCCGCCATTGTTTTGAAGAAGGGAGAAAAAATGACCTACGAAGAACTGGTGAACTTCTTAAAGAAGCACCTAGCCTCTTTCAAAATTCCAACCAAGGTGTACTTTGTTGATAAGCTACCAAAAACCGCTACAGGTAAAATCCAGAGAAGAGTTATCGCAGAAACTTTTGCTAAGAGCAGCAGAAATAAGAGTAAGTTG
SEQ ID NO:112(核糖体结合位点RBS1和Of_oxc开放读码框):
GCATCCCCAAAAAATTAATTCATTTTCTCTTTTCCCTAACCCTTAAAATATAATAAAATGAGTAACGACGACAATGTAGAGTTGACTGATGGCTTTCATGTTTTGATCGATGCCCTGAAAATGAATGACATCGATACCATGTATGGTGTTGTCGGCATTCCTATCACGAACCTGGCTCGTATGTGGCAAGATGACGGTCAGCGTTTTTACAGCTTCCGTCACGAACAACACGCAGGTTATGCAGCTTCTATCGCCGGTTACATCGAAGGAAAACCTGGCGTTTGCTTGACCGTTTCCGCCCCTGGCTTCCTGAACGGCGTGACTTCCCTGGCTCATGCAACCACCAACTGCTTCCCAATGATCCTGTTGAGCGGTTCCAGTGAACGTGAAATCGTCGATTTGCAACAGGGCGATTACGAAGAAATGGATCAGATGAATGTTGCACGTCCACACTGCAAAGCTTCTTTCCGTATCAACAGCATCAAAGACATTCCAATCGGTATCGCTCGTGCAGTTCGCACCGCTGTATCCGGACGTCCAGGTGGTGTTTACGTTGACTTGCCAGCAAAACTGTTCGGTCAGACCATTTCTGTAGAAGAAGCTAACAAACTGCTCTTCAAACCAATCGATCCAGCTCCGGCACAGATTCCTGCTGAAGATGCTATCGCTCGCGCTGCTGACCTGATCAAGAACGCCAAACGTCCAGTTATCATGCTGGGTAAAGGCGCTGCATACGCACAATGCGACGACGAAATCCGCGCACTGGTTGAAGAAACCGGCATCCCATTCCTGCCAATGGGTATGGCTAAAGGCCTGCTGCCTGACAACCATCCACAATCCGCTGCTGCAACCCGTGCTTTCGCACTGGCACAGTGTGACGTTTGCGTACTGATCGGCGCTCGTCTGAACTGGCTGATGCAGCACGGTAAAGGCAAAACCTGGGGCGACGAACTGAAGAAATACGTTCAGATCGACATCCAGGCTAACGAAATGGACAGCAACCAGCCTATCGCTGCACCAGTTGTTGGTGACATCAAGTCCGCCGTTTCCCTGCTCCGCAAAGCACTGAAAGGCGCTCCAAAAGCTGACGCTGAATGGACCGGCGCTCTGAAAGCCAAAGTTGACGGCAACAAAGCCAAACTGGCTGGCAAGATGACTGCCGAAACCCCATCCGGAATGATGAACTACTCCAATTCCCTGGGCGTTGTTCGTGACTTCATGCTGGCAAATCCGGATATTTCCCTGGTTAACGAAGGCGCTAATGCACTCGACAACACTCGTATGATTGTTGACATGCTGAAACCACGCAAACGTCTTGACTCCGGTACCTGGGGTGTTATGGGTATTGGTATGGGCTACTGCGTTGCTGCAGCTGCTGTTACCGGCAAACCGGTTATCGCTGTTGAAGGCGATAGCGCATTCGGTTTCTCCGGTATGGAACTGGAAACCATCTGCCGTTACAACCTGCCAGTTACCGTTATCATCATGAACAATGGTGGTATCTATAAAGGTAACGAAGCAGATCCACAACCAGGCGTTATCTCCTGTACCCGTCTGACCCGTGGTCGTTACGACATGATGATGGAAGCATTTGGCGGTAAAGGTTATGTTGCCAATACTCCAGCAGAACTGAAAGCTGCTCTGGAAGAAGCTGTTGCTTCCGGCAAACCATGCCTGATCAACGCGATGATCGATCCAGACGCTGGTGTCGAATCTGGCCGTATCAAGAGCCTGAACGTTGTAAGTAAAGTTGGCAAGAAA
SEQ ID NO:113(核糖体结合位点RBS2和Of_oxc开放读码框)：
GCATCAACTACCTAAATTAATTATCACCCCAAATTAATAACATAAAAATATAATAAAATGAGTAACGACGACAATGTAGAGTTGACTGATGGCTTTCATGTTTTGATCGATGCCCTGAAAATGAATGACATCGATACCATGTATGGTGTTGTCGGCATTCCTATCACGAACCTGGCTCGTATGTGGCAAGATGACGGTCAGCGTTTTTACAGCTTCCGTCACGAACAACACGCAGGTTATGCAGCTTCTATCGCCGGTTACATCGAAGGAAAACCTGGCGTTTGCTTGACCGTTTCCGCCCCTGGCTTCCTGAACGGCGTGACTTCCCTGGCTCATGCAACCACCAACTGCTTCCCAATGATCCTGTTGAGCGGTTCCAGTGAACGTGAAATCGTCGATTTGCAACAGGGCGATTACGAAGAAATGGATCAGATGAATGTTGCACGTCCACACTGCAAAGCTTCTTTCCGTATCAACAGCATCAAAGACATTCCAATCGGTATCGCTCGTGCAGTTCGCACCGCTGTATCCGGACGTCCAGGTGGTGTTTACGTTGACTTGCCAGCAAAACTGTTCGGTCAGACCATTTCTGTAGAAGAAGCTAACAAACTGCTCTTCAAACCAATCGATCCAGCTCCGGCACAGATTCCTGCTGAAGATGCTATCGCTCGCGCTGCTGACCTGATCAAGAACGCCAAACGTCCAGTTATCATGCTGGGTAAAGGCGCTGCATACGCACAATGCGACGACGAAATCCGCGCACTGGTTGAAGAAACCGGCATCCCATTCCTGCCAATGGGTATGGCTAAAGGCCTGCTGCCTGACAACCATCCACAATCCGCTGCTGCAACCCGTGCTTTCGCACTGGCACAGTGTGACGTTTGCGTACTGATCGGCGCTCGTCTGAACTGGCTGATGCAGCACGGTAAAGGCAAAACCTGGGGCGACGAACTGAAGAAATACGTTCAGATCGACATCCAGGCTAACGAAATGGACAGCAACCAGCCTATCGCTGCACCAGTTGTTGGTGACATCAAGTCCGCCGTTTCCCTGCTCCGCAAAGCACTGAAAGGCGCTCCAAAAGCTGACGCTGAATGGACCGGCGCTCTGAAAGCCAAAGTTGACGGCAACAAAGCCAAACTGGCTGGCAAGATGACTGCCGAAACCCCATCCGGAATGATGAACTACTCCAATTCCCTGGGCGTTGTTCGTGACTTCATGCTGGCAAATCCGGATATTTCCCTGGTTAACGAAGGCGCTAATGCACTCGACAACACTCGTATGATTGTTGACATGCTGAAACCACGCAAACGTCTTGACTCCGGTACCTGGGGTGTTATGGGTATTGGTATGGGCTACTGCGTTGCTGCAGCTGCTGTTACCGGCAAACCGGTTATCGCTGTTGAAGGCGATAGCGCATTCGGTTTCTCCGGTATGGAACTGGAAACCATCTGCCGTTACAACCTGCCAGTTACCGTTATCATCATGAACAATGGTGGTATCTATAAAGGTAACGAAGCAGATCCACAACCAGGCGTTATCTCCTGTACCCGTCTGACCCGTGGTCGTTACGACATGATGATGGAAGCATTTGGCGGTAAAGGTTATGTTGCCAATACTCCAGCAGAACTGAAAGCTGCTCTGGAAGAAGCTGTTGCTTCCGGCAAACCATGCCTGATCAACGCGATGATCGATCCAGACGCTGGTGTCGAATCTGGCCGTATCAAGAGCCTGAACGTTGTAAGTAAAGTTGGCAAGAAA
SEQ ID NO:114(核糖体结合位点RBS3和Of_oxc开放读码框)：
GCATCAACTACCTAAATTAATTATCACCCCAAATTAATAACATAAAAATATAATAAAATGAGTAACGACGACAATGTAGAGTTGACTGATGGCTTTCATGTTTTGATCGATGCCCTGAAAATGAATGACATCGATACCATGTATGGTGTTGTCGGCATTCCTATCACGAACCTGGCTCGTATGTGGCAAGATGACGGTCAGCGTTTTTACAGCTTCCGTCACGAACAACACGCAGGTTATGCAGCTTCTATCGCCGGTTACATCGAAGGAAAACCTGGCGTTTGCTTGACCGTTTCCGCCCCTGGCTTCCTGAACGGCGTGACTTCCCTGGCTCATGCAACCACCAACTGCTTCCCAATGATCCTGTTGAGCGGTTCCAGTGAACGTGAAATCGTCGATTTGCAACAGGGCGATTACGAAGAAATGGATCAGATGAATGTTGCACGTCCACACTGCAAAGCTTCTTTCCGTATCAACAGCATCAAAGACATTCCAATCGGTATCGCTCGTGCAGTTCGCACCGCTGTATCCGGACGTCCAGGTGGTGTTTACGTTGACTTGCCAGCAAAACTGTTCGGTCAGACCATTTCTGTAGAAGAAGCTAACAAACTGCTCTTCAAACCAATCGATCCAGCTCCGGCACAGATTCCTGCTGAAGATGCTATCGCTCGCGCTGCTGACCTGATCAAGAACGCCAAACGTCCAGTTATCATGCTGGGTAAAGGCGCTGCATACGCACAATGCGACGACGAAATCCGCGCACTGGTTGAAGAAACCGGCATCCCATTCCTGCCAATGGGTATGGCTAAAGGCCTGCTGCCTGACAACCATCCACAATCCGCTGCTGCAACCCGTGCTTTCGCACTGGCACAGTGTGACGTTTGCGTACTGATCGGCGCTCGTCTGAACTGGCTGATGCAGCACGGTAAAGGCAAAACCTGGGGCGACGAACTGAAGAAATACGTTCAGATCGACATCCAGGCTAACGAAATGGACAGCAACCAGCCTATCGCTGCACCAGTTGTTGGTGACATCAAGTCCGCCGTTTCCCTGCTCCGCAAAGCACTGAAAGGCGCTCCAAAAGCTGACGCTGAATGGACCGGCGCTCTGAAAGCCAAAGTTGACGGCAACAAAGCCAAACTGGCTGGCAAGATGACTGCCGAAACCCCATCCGGAATGATGAACTACTCCAATTCCCTGGGCGTTGTTCGTGACTTCATGCTGGCAAATCCGGATATTTCCCTGGTTAACGAAGGCGCTAATGCACTCGACAACACTCGTATGATTGTTGACATGCTGAAACCACGCAAACGTCTTGACTCCGGTACCTGGGGTGTTATGGGTATTGGTATGGGCTACTGCGTTGCTGCAGCTGCTGTTACCGGCAAACCGGTTATCGCTGTTGAAGGCGATAGCGCATTCGGTTTCTCCGGTATGGAACTGGAAACCATCTGCCGTTACAACCTGCCAGTTACCGTTATCATCATGAACAATGGTGGTATCTATAAAGGTAACGAAGCAGATCCACAACCAGGCGTTATCTCCTGTACCCGTCTGACCCGTGGTCGTTACGACATGATGATGGAAGCATTTGGCGGTAAAGGTTATGTTGCCAATACTCCAGCAGAACTGAAAGCTGCTCTGGAAGAAGCTGTTGCTTCCGGCAAACCATGCCTGATCAACGCGATGATCGATCCAGACGCTGGTGTCGAATCTGGCCGTATCAAGAGCCTGAACGTTGTAAGTAAAGTTGGCAAGAAA
SEQ ID NO:115(核糖体结合位点RBS4和Of_oxc开放读码框)：
GCATCCCCAAAAAATTAATTCATTTTCTCTTTTCAACAATTTAAAAAAAATTAAAAAATGAGTAACGACGACAATGTAGAGTTGACTGATGGCTTTCATGTTTTGATCGATGCCCTGAAAATGAATGACATCGATACCATGTATGGTGTTGTCGGCATTCCTATCACGAACCTGGCTCGTATGTGGCAAGATGACGGTCAGCGTTTTTACAGCTTCCGTCACGAACAACACGCAGGTTATGCAGCTTCTATCGCCGGTTACATCGAAGGAAAACCTGGCGTTTGCTTGACCGTTTCCGCCCCTGGCTTCCTGAACGGCGTGACTTCCCTGGCTCATGCAACCACCAACTGCTTCCCAATGATCCTGTTGAGCGGTTCCAGTGAACGTGAAATCGTCGATTTGCAACAGGGCGATTACGAAGAAATGGATCAGATGAATGTTGCACGTCCACACTGCAAAGCTTCTTTCCGTATCAACAGCATCAAAGACATTCCAATCGGTATCGCTCGTGCAGTTCGCACCGCTGTATCCGGACGTCCAGGTGGTGTTTACGTTGACTTGCCAGCAAAACTGTTCGGTCAGACCATTTCTGTAGAAGAAGCTAACAAACTGCTCTTCAAACCAATCGATCCAGCTCCGGCACAGATTCCTGCTGAAGATGCTATCGCTCGCGCTGCTGACCTGATCAAGAACGCCAAACGTCCAGTTATCATGCTGGGTAAAGGCGCTGCATACGCACAATGCGACGACGAAATCCGCGCACTGGTTGAAGAAACCGGCATCCCATTCCTGCCAATGGGTATGGCTAAAGGCCTGCTGCCTGACAACCATCCACAATCCGCTGCTGCAACCCGTGCTTTCGCACTGGCACAGTGTGACGTTTGCGTACTGATCGGCGCTCGTCTGAACTGGCTGATGCAGCACGGTAAAGGCAAAACCTGGGGCGACGAACTGAAGAAATACGTTCAGATCGACATCCAGGCTAACGAAATGGACAGCAACCAGCCTATCGCTGCACCAGTTGTTGGTGACATCAAGTCCGCCGTTTCCCTGCTCCGCAAAGCACTGAAAGGCGCTCCAAAAGCTGACGCTGAATGGACCGGCGCTCTGAAAGCCAAAGTTGACGGCAACAAAGCCAAACTGGCTGGCAAGATGACTGCCGAAACCCCATCCGGAATGATGAACTACTCCAATTCCCTGGGCGTTGTTCGTGACTTCATGCTGGCAAATCCGGATATTTCCCTGGTTAACGAAGGCGCTAATGCACTCGACAACACTCGTATGATTGTTGACATGCTGAAACCACGCAAACGTCTTGACTCCGGTACCTGGGGTGTTATGGGTATTGGTATGGGCTACTGCGTTGCTGCAGCTGCTGTTACCGGCAAACCGGTTATCGCTGTTGAAGGCGATAGCGCATTCGGTTTCTCCGGTATGGAACTGGAAACCATCTGCCGTTACAACCTGCCAGTTACCGTTATCATCATGAACAATGGTGGTATCTATAAAGGTAACGAAGCAGATCCACAACCAGGCGTTATCTCCTGTACCCGTCTGACCCGTGGTCGTTACGACATGATGATGGAAGCATTTGGCGGTAAAGGTTATGTTGCCAATACTCCAGCAGAACTGAAAGCTGCTCTGGAAGAAGCTGTTGCTTCCGGCAAACCATGCCTGATCAACGCGATGATCGATCCAGACGCTGGTGTCGAATCTGGCCGTATCAAGAGCCTGAACGTTGTAAGTAAAGTTGGCAAGAAA
SEQ ID NO:116(核糖体结合位点RBS5和Of_oxc开放读码框)：
GCATCCCCAAAAAATTAATTCATTTTCTCTTTTCGATCCTATAAAAAATATAACAAAATGAGTAACGACGACAATGTAGAGTTGACTGATGGCTTTCATGTTTTGATCGATGCCCTGAAAATGAATGACATCGATACCATGTATGGTGTTGTCGGCATTCCTATCACGAACCTGGCTCGTATGTGGCAAGATGACGGTCAGCGTTTTTACAGCTTCCGTCACGAACAACACGCAGGTTATGCAGCTTCTATCGCCGGTTACATCGAAGGAAAACCTGGCGTTTGCTTGACCGTTTCCGCCCCTGGCTTCCTGAACGGCGTGACTTCCCTGGCTCATGCAACCACCAACTGCTTCCCAATGATCCTGTTGAGCGGTTCCAGTGAACGTGAAATCGTCGATTTGCAACAGGGCGATTACGAAGAAATGGATCAGATGAATGTTGCACGTCCACACTGCAAAGCTTCTTTCCGTATCAACAGCATCAAAGACATTCCAATCGGTATCGCTCGTGCAGTTCGCACCGCTGTATCCGGACGTCCAGGTGGTGTTTACGTTGACTTGCCAGCAAAACTGTTCGGTCAGACCATTTCTGTAGAAGAAGCTAACAAACTGCTCTTCAAACCAATCGATCCAGCTCCGGCACAGATTCCTGCTGAAGATGCTATCGCTCGCGCTGCTGACCTGATCAAGAACGCCAAACGTCCAGTTATCATGCTGGGTAAAGGCGCTGCATACGCACAATGCGACGACGAAATCCGCGCACTGGTTGAAGAAACCGGCATCCCATTCCTGCCAATGGGTATGGCTAAAGGCCTGCTGCCTGACAACCATCCACAATCCGCTGCTGCAACCCGTGCTTTCGCACTGGCACAGTGTGACGTTTGCGTACTGATCGGCGCTCGTCTGAACTGGCTGATGCAGCACGGTAAAGGCAAAACCTGGGGCGACGAACTGAAGAAATACGTTCAGATCGACATCCAGGCTAACGAAATGGACAGCAACCAGCCTATCGCTGCACCAGTTGTTGGTGACATCAAGTCCGCCGTTTCCCTGCTCCGCAAAGCACTGAAAGGCGCTCCAAAAGCTGACGCTGAATGGACCGGCGCTCTGAAAGCCAAAGTTGACGGCAACAAAGCCAAACTGGCTGGCAAGATGACTGCCGAAACCCCATCCGGAATGATGAACTACTCCAATTCCCTGGGCGTTGTTCGTGACTTCATGCTGGCAAATCCGGATATTTCCCTGGTTAACGAAGGCGCTAATGCACTCGACAACACTCGTATGATTGTTGACATGCTGAAACCACGCAAACGTCTTGACTCCGGTACCTGGGGTGTTATGGGTATTGGTATGGGCTACTGCGTTGCTGCAGCTGCTGTTACCGGCAAACCGGTTATCGCTGTTGAAGGCGATAGCGCATTCGGTTTCTCCGGTATGGAACTGGAAACCATCTGCCGTTACAACCTGCCAGTTACCGTTATCATCATGAACAATGGTGGTATCTATAAAGGTAACGAAGCAGATCCACAACCAGGCGTTATCTCCTGTACCCGTCTGACCCGTGGTCGTTACGACATGATGATGGAAGCATTTGGCGGTAAAGGTTATGTTGCCAATACTCCAGCAGAACTGAAAGCTGCTCTGGAAGAAGCTGTTGCTTCCGGCAAACCATGCCTGATCAACGCGATGATCGATCCAGACGCTGGTGTCGAATCTGGCCGTATCAAGAGCCTGAACGTTGTAAGTAAAGTTGGCAAGAAA
SEQ ID NO:117(核糖体结合位点RBS6和Of_oxc开放读码框)：
GCATCATCTACCTTTATTATTAATCAATCCATCTCAGTAAGTTTAAAATATAACAAAATGAGTAACGACGACAATGTAGAGTTGACTGATGGCTTTCATGTTTTGATCGATGCCCTGAAAATGAATGACATCGATACCATGTATGGTGTTGTCGGCATTCCTATCACGAACCTGGCTCGTATGTGGCAAGATGACGGTCAGCGTTTTTACAGCTTCCGTCACGAACAACACGCAGGTTATGCAGCTTCTATCGCCGGTTACATCGAAGGAAAACCTGGCGTTTGCTTGACCGTTTCCGCCCCTGGCTTCCTGAACGGCGTGACTTCCCTGGCTCATGCAACCACCAACTGCTTCCCAATGATCCTGTTGAGCGGTTCCAGTGAACGTGAAATCGTCGATTTGCAACAGGGCGATTACGAAGAAATGGATCAGATGAATGTTGCACGTCCACACTGCAAAGCTTCTTTCCGTATCAACAGCATCAAAGACATTCCAATCGGTATCGCTCGTGCAGTTCGCACCGCTGTATCCGGACGTCCAGGTGGTGTTTACGTTGACTTGCCAGCAAAACTGTTCGGTCAGACCATTTCTGTAGAAGAAGCTAACAAACTGCTCTTCAAACCAATCGATCCAGCTCCGGCACAGATTCCTGCTGAAGATGCTATCGCTCGCGCTGCTGACCTGATCAAGAACGCCAAACGTCCAGTTATCATGCTGGGTAAAGGCGCTGCATACGCACAATGCGACGACGAAATCCGCGCACTGGTTGAAGAAACCGGCATCCCATTCCTGCCAATGGGTATGGCTAAAGGCCTGCTGCCTGACAACCATCCACAATCCGCTGCTGCAACCCGTGCTTTCGCACTGGCACAGTGTGACGTTTGCGTACTGATCGGCGCTCGTCTGAACTGGCTGATGCAGCACGGTAAAGGCAAAACCTGGGGCGACGAACTGAAGAAATACGTTCAGATCGACATCCAGGCTAACGAAATGGACAGCAACCAGCCTATCGCTGCACCAGTTGTTGGTGACATCAAGTCCGCCGTTTCCCTGCTCCGCAAAGCACTGAAAGGCGCTCCAAAAGCTGACGCTGAATGGACCGGCGCTCTGAAAGCCAAAGTTGACGGCAACAAAGCCAAACTGGCTGGCAAGATGACTGCCGAAACCCCATCCGGAATGATGAACTACTCCAATTCCCTGGGCGTTGTTCGTGACTTCATGCTGGCAAATCCGGATATTTCCCTGGTTAACGAAGGCGCTAATGCACTCGACAACACTCGTATGATTGTTGACATGCTGAAACCACGCAAACGTCTTGACTCCGGTACCTGGGGTGTTATGGGTATTGGTATGGGCTACTGCGTTGCTGCAGCTGCTGTTACCGGCAAACCGGTTATCGCTGTTGAAGGCGATAGCGCATTCGGTTTCTCCGGTATGGAACTGGAAACCATCTGCCGTTACAACCTGCCAGTTACCGTTATCATCATGAACAATGGTGGTATCTATAAAGGTAACGAAGCAGATCCACAACCAGGCGTTATCTCCTGTACCCGTCTGACCCGTGGTCGTTACGACATGATGATGGAAGCATTTGGCGGTAAAGGTTATGTTGCCAATACTCCAGCAGAACTGAAAGCTGCTCTGGAAGAAGCTGTTGCTTCCGGCAAACCATGCCTGATCAACGCGATGATCGATCCAGACGCTGGTGTCGAATCTGGCCGTATCAAGAGCCTGAACGTTGTAAGTAAAGTTGGCAAGAAA
SEQ ID NO:118(核糖体结合位点RBS7和Of_oxc开放读码框)：
GCATCAACTACCTCTATTAATAATCAATCCACATCTTGATCGTAAAAAATTTAAAAAATGAGTAACGACGACAATGTAGAGTTGACTGATGGCTTTCATGTTTTGATCGATGCCCTGAAAATGAATGACATCGATACCATGTATGGTGTTGTCGGCATTCCTATCACGAACCTGGCTCGTATGTGGCAAGATGACGGTCAGCGTTTTTACAGCTTCCGTCACGAACAACACGCAGGTTATGCAGCTTCTATCGCCGGTTACATCGAAGGAAAACCTGGCGTTTGCTTGACCGTTTCCGCCCCTGGCTTCCTGAACGGCGTGACTTCCCTGGCTCATGCAACCACCAACTGCTTCCCAATGATCCTGTTGAGCGGTTCCAGTGAACGTGAAATCGTCGATTTGCAACAGGGCGATTACGAAGAAATGGATCAGATGAATGTTGCACGTCCACACTGCAAAGCTTCTTTCCGTATCAACAGCATCAAAGACATTCCAATCGGTATCGCTCGTGCAGTTCGCACCGCTGTATCCGGACGTCCAGGTGGTGTTTACGTTGACTTGCCAGCAAAACTGTTCGGTCAGACCATTTCTGTAGAAGAAGCTAACAAACTGCTCTTCAAACCAATCGATCCAGCTCCGGCACAGATTCCTGCTGAAGATGCTATCGCTCGCGCTGCTGACCTGATCAAGAACGCCAAACGTCCAGTTATCATGCTGGGTAAAGGCGCTGCATACGCACAATGCGACGACGAAATCCGCGCACTGGTTGAAGAAACCGGCATCCCATTCCTGCCAATGGGTATGGCTAAAGGCCTGCTGCCTGACAACCATCCACAATCCGCTGCTGCAACCCGTGCTTTCGCACTGGCACAGTGTGACGTTTGCGTACTGATCGGCGCTCGTCTGAACTGGCTGATGCAGCACGGTAAAGGCAAAACCTGGGGCGACGAACTGAAGAAATACGTTCAGATCGACATCCAGGCTAACGAAATGGACAGCAACCAGCCTATCGCTGCACCAGTTGTTGGTGACATCAAGTCCGCCGTTTCCCTGCTCCGCAAAGCACTGAAAGGCGCTCCAAAAGCTGACGCTGAATGGACCGGCGCTCTGAAAGCCAAAGTTGACGGCAACAAAGCCAAACTGGCTGGCAAGATGACTGCCGAAACCCCATCCGGAATGATGAACTACTCCAATTCCCTGGGCGTTGTTCGTGACTTCATGCTGGCAAATCCGGATATTTCCCTGGTTAACGAAGGCGCTAATGCACTCGACAACACTCGTATGATTGTTGACATGCTGAAACCACGCAAACGTCTTGACTCCGGTACCTGGGGTGTTATGGGTATTGGTATGGGCTACTGCGTTGCTGCAGCTGCTGTTACCGGCAAACCGGTTATCGCTGTTGAAGGCGATAGCGCATTCGGTTTCTCCGGTATGGAACTGGAAACCATCTGCCGTTACAACCTGCCAGTTACCGTTATCATCATGAACAATGGTGGTATCTATAAAGGTAACGAAGCAGATCCACAACCAGGCGTTATCTCCTGTACCCGTCTGACCCGTGGTCGTTACGACATGATGATGGAAGCATTTGGCGGTAAAGGTTATGTTGCCAATACTCCAGCAGAACTGAAAGCTGCTCTGGAAGAAGCTGTTGCTTCCGGCAAACCATGCCTGATCAACGCGATGATCGATCCAGACGCTGGTGTCGAATCTGGCCGTATCAAGAGCCTGAACGTTGTAAGTAAAGTTGGCAAGAAA
SEQ ID NO:119(核糖体结合位点RBS8和Of_oxc开放读码框)：
GCATCACCTACCTAAATTACTAAACAAACCACCTGCATTTGTTTTAAAAATTACAAAATGAGTAACGACGACAATGTAGAGTTGACTGATGGCTTTCATGTTTTGATCGATGCCCTGAAAATGAATGACATCGATACCATGTATGGTGTTGTCGGCATTCCTATCACGAACCTGGCTCGTATGTGGCAAGATGACGGTCAGCGTTTTTACAGCTTCCGTCACGAACAACACGCAGGTTATGCAGCTTCTATCGCCGGTTACATCGAAGGAAAACCTGGCGTTTGCTTGACCGTTTCCGCCCCTGGCTTCCTGAACGGCGTGACTTCCCTGGCTCATGCAACCACCAACTGCTTCCCAATGATCCTGTTGAGCGGTTCCAGTGAACGTGAAATCGTCGATTTGCAACAGGGCGATTACGAAGAAATGGATCAGATGAATGTTGCACGTCCACACTGCAAAGCTTCTTTCCGTATCAACAGCATCAAAGACATTCCAATCGGTATCGCTCGTGCAGTTCGCACCGCTGTATCCGGACGTCCAGGTGGTGTTTACGTTGACTTGCCAGCAAAACTGTTCGGTCAGACCATTTCTGTAGAAGAAGCTAACAAACTGCTCTTCAAACCAATCGATCCAGCTCCGGCACAGATTCCTGCTGAAGATGCTATCGCTCGCGCTGCTGACCTGATCAAGAACGCCAAACGTCCAGTTATCATGCTGGGTAAAGGCGCTGCATACGCACAATGCGACGACGAAATCCGCGCACTGGTTGAAGAAACCGGCATCCCATTCCTGCCAATGGGTATGGCTAAAGGCCTGCTGCCTGACAACCATCCACAATCCGCTGCTGCAACCCGTGCTTTCGCACTGGCACAGTGTGACGTTTGCGTACTGATCGGCGCTCGTCTGAACTGGCTGATGCAGCACGGTAAAGGCAAAACCTGGGGCGACGAACTGAAGAAATACGTTCAGATCGACATCCAGGCTAACGAAATGGACAGCAACCAGCCTATCGCTGCACCAGTTGTTGGTGACATCAAGTCCGCCGTTTCCCTGCTCCGCAAAGCACTGAAAGGCGCTCCAAAAGCTGACGCTGAATGGACCGGCGCTCTGAAAGCCAAAGTTGACGGCAACAAAGCCAAACTGGCTGGCAAGATGACTGCCGAAACCCCATCCGGAATGATGAACTACTCCAATTCCCTGGGCGTTGTTCGTGACTTCATGCTGGCAAATCCGGATATTTCCCTGGTTAACGAAGGCGCTAATGCACTCGACAACACTCGTATGATTGTTGACATGCTGAAACCACGCAAACGTCTTGACTCCGGTACCTGGGGTGTTATGGGTATTGGTATGGGCTACTGCGTTGCTGCAGCTGCTGTTACCGGCAAACCGGTTATCGCTGTTGAAGGCGATAGCGCATTCGGTTTCTCCGGTATGGAACTGGAAACCATCTGCCGTTACAACCTGCCAGTTACCGTTATCATCATGAACAATGGTGGTATCTATAAAGGTAACGAAGCAGATCCACAACCAGGCGTTATCTCCTGTACCCGTCTGACCCGTGGTCGTTACGACATGATGATGGAAGCATTTGGCGGTAAAGGTTATGTTGCCAATACTCCAGCAGAACTGAAAGCTGCTCTGGAAGAAGCTGTTGCTTCCGGCAAACCATGCCTGATCAACGCGATGATCGATCCAGACGCTGGTGTCGAATCTGGCCGTATCAAGAGCCTGAACGTTGTAAGTAAAGTTGGCAAGAAA
SEQ ID NO:120(核糖体结合位点RBS1和Sc_YEL020C开放读码框)：
GCATCAACTACCTAAATTAATCATCAAACCAACTAGAATATGAAAAAAAATAAAAAAATGACCACGACCGCTACCCAACACTTCGCTCAACTTTTACAAAAATATGGCATCGACACAGTCTTTGGAATTGTGGGCATCCCTATTGTCCAACTAGCGGATACGATGGTTGCCAACGGTATCAAATTCATTCCATGCAGGAACGAACAGGCTGCCTCATATGCAGCCTCTGCTTATGGTTATATTAGTGATAAGCCCGGCGTGTTACTTATTGTCGGGGGCCCCGGTTTAATCCACGCACTGGCTGGTATATACAACTCAATGAGTAATAGGTGGCCTCTTCTAGTAATTGCAGGGAGTTCTTCTCAAAGTGATATACATAAAGGCGGGTTTCAAGAGTTGGATCAAGTTAGTCTTTTGTCCCCATTTTTGAAGTTTACCGGAAAGTTAACCCCTGACAATATTGACATGATTACTCAGAAGGCTTTGAATTACTGTATACAAGGCACTGCAGGGGTTTCTTACATAGACGTCCCTGCAGATTTTATTGAGTATGAAAAACCCTTAGAAGGAAACGACCGTACAGGAAATGAATTACCGATGATATTAACTCCAAACATATGTGGCCCTGATCCGTCGAAGATCAAAAAAGTCGTACAGCTTATTTTGCAACATAAAAATAAAAATATTCTGATTGTTATTGGAAAGGGTGCGGTAAAAAACTCACACGAAATCCGCAGACTAGTAAATACGTTCAATCTACCATTTTTACCCACCCCAATGGCTAAGGGAATTGTCCCAGACTCTTCTCCACTGAACGTTTCATCTGCAAGATCTCAAGCATTGAAAATAGCGGATATTGTTCTTGTTCTCGGGGCAAGATTAAACTGGATATTGCATTTTGGTACTTCACCCAAATGGAACTCAGAATCCATATTCATCCAGTTCGATTCCAATCCCGAAACCTTGGGGGATAACAATGTCTCACCTGGTGCTGACCTTTCCATATGGGGAGATATAGGCTTGAGCGTAACCGCTTTAGTTGAGGAATTGACTCGCCAAGATTCCTGCTGGAAGTATAGTGGTGTTAAGCAAGAAATACGAGAGAAAATTCAACTGAATCAAACTCGTCTATTGAGGAAAGAGAAGACTAGAGGGGCACAATTGAATTACAACCAGGTTTATGGAACATTGAGGCCTCTTATCGATGATTACAGGACAATACTTGTGACGGAGGGAGCAAATACTATGGATATTGCGCGTATTTCATTCCCTACAGACGCTCCAAGGCGCCGTTTAGACGCAGGGACCAACGCGACTATGGGGATTGGGCTCGGATATGCTCTTGCATGCAAGGCATCTCATCCGGAACTCGATGTGGTGCTGATTCAGGGTGATTCCGCATTTGGATTCTCTGCCATGGAAATTGAAACGGCAGTAAGGTGTCAGCTGGCATTGGTCATCGTTGTGATGAACAATAGCGGTATTTACCATGGAGAAAAGGATATAGAGGGTGATTTACCTCCAACGGCACTAAGCAAGAACTGCCGTTACGATCTCGTAGGCAAAGGACTGGGTGCCAATGATTTTTTTGTCAACACAATAAGCGAACTGAGCAGATGCTTCCAACAAGCCGTGCAGCTTTCTCGGACTAAAAGAGAAACAAGTGTCATCAACGTTATCATTGAGCCTGGCGAACAAAAGCAAATTGCCTTTGCCTGGCAGAATAAACCGCGTTTA
SEQ ID NO:121(核糖体结合位点RBS2和Sc_YEL020C开放读码框)：
GCATCAACTACCTCAATTAATAAACAAACCAACTGTCCAATGTATAAAATTAAAAAAATGACCACGACCGCTACCCAACACTTCGCTCAACTTTTACAAAAATATGGCATCGACACAGTCTTTGGAATTGTGGGCATCCCTATTGTCCAACTAGCGGATACGATGGTTGCCAACGGTATCAAATTCATTCCATGCAGGAACGAACAGGCTGCCTCATATGCAGCCTCTGCTTATGGTTATATTAGTGATAAGCCCGGCGTGTTACTTATTGTCGGGGGCCCCGGTTTAATCCACGCACTGGCTGGTATATACAACTCAATGAGTAATAGGTGGCCTCTTCTAGTAATTGCAGGGAGTTCTTCTCAAAGTGATATACATAAAGGCGGGTTTCAAGAGTTGGATCAAGTTAGTCTTTTGTCCCCATTTTTGAAGTTTACCGGAAAGTTAACCCCTGACAATATTGACATGATTACTCAGAAGGCTTTGAATTACTGTATACAAGGCACTGCAGGGGTTTCTTACATAGACGTCCCTGCAGATTTTATTGAGTATGAAAAACCCTTAGAAGGAAACGACCGTACAGGAAATGAATTACCGATGATATTAACTCCAAACATATGTGGCCCTGATCCGTCGAAGATCAAAAAAGTCGTACAGCTTATTTTGCAACATAAAAATAAAAATATTCTGATTGTTATTGGAAAGGGTGCGGTAAAAAACTCACACGAAATCCGCAGACTAGTAAATACGTTCAATCTACCATTTTTACCCACCCCAATGGCTAAGGGAATTGTCCCAGACTCTTCTCCACTGAACGTTTCATCTGCAAGATCTCAAGCATTGAAAATAGCGGATATTGTTCTTGTTCTCGGGGCAAGATTAAACTGGATATTGCATTTTGGTACTTCACCCAAATGGAACTCAGAATCCATATTCATCCAGTTCGATTCCAATCCCGAAACCTTGGGGGATAACAATGTCTCACCTGGTGCTGACCTTTCCATATGGGGAGATATAGGCTTGAGCGTAACCGCTTTAGTTGAGGAATTGACTCGCCAAGATTCCTGCTGGAAGTATAGTGGTGTTAAGCAAGAAATACGAGAGAAAATTCAACTGAATCAAACTCGTCTATTGAGGAAAGAGAAGACTAGAGGGGCACAATTGAATTACAACCAGGTTTATGGAACATTGAGGCCTCTTATCGATGATTACAGGACAATACTTGTGACGGAGGGAGCAAATACTATGGATATTGCGCGTATTTCATTCCCTACAGACGCTCCAAGGCGCCGTTTAGACGCAGGGACCAACGCGACTATGGGGATTGGGCTCGGATATGCTCTTGCATGCAAGGCATCTCATCCGGAACTCGATGTGGTGCTGATTCAGGGTGATTCCGCATTTGGATTCTCTGCCATGGAAATTGAAACGGCAGTAAGGTGTCAGCTGGCATTGGTCATCGTTGTGATGAACAATAGCGGTATTTACCATGGAGAAAAGGATATAGAGGGTGATTTACCTCCAACGGCACTAAGCAAGAACTGCCGTTACGATCTCGTAGGCAAAGGACTGGGTGCCAATGATTTTTTTGTCAACACAATAAGCGAACTGAGCAGATGCTTCCAACAAGCCGTGCAGCTTTCTCGGACTAAAAGAGAAACAAGTGTCATCAACGTTATCATTGAGCCTGGCGAACAAAAGCAAATTGCCTTTGCCTGGCAGAATAAACCGCGTTTA
SEQ ID NO:122(核糖体结合位点RBS3和Sc_YEL020C开放读码框)：
GCATCAACTACCTCAATTACTAAACAATCCACATTATTAATATAAAAATATAAAAAAATGACCACGACCGCTACCCAACACTTCGCTCAACTTTTACAAAAATATGGCATCGACACAGTCTTTGGAATTGTGGGCATCCCTATTGTCCAACTAGCGGATACGATGGTTGCCAACGGTATCAAATTCATTCCATGCAGGAACGAACAGGCTGCCTCATATGCAGCCTCTGCTTATGGTTATATTAGTGATAAGCCCGGCGTGTTACTTATTGTCGGGGGCCCCGGTTTAATCCACGCACTGGCTGGTATATACAACTCAATGAGTAATAGGTGGCCTCTTCTAGTAATTGCAGGGAGTTCTTCTCAAAGTGATATACATAAAGGCGGGTTTCAAGAGTTGGATCAAGTTAGTCTTTTGTCCCCATTTTTGAAGTTTACCGGAAAGTTAACCCCTGACAATATTGACATGATTACTCAGAAGGCTTTGAATTACTGTATACAAGGCACTGCAGGGGTTTCTTACATAGACGTCCCTGCAGATTTTATTGAGTATGAAAAACCCTTAGAAGGAAACGACCGTACAGGAAATGAATTACCGATGATATTAACTCCAAACATATGTGGCCCTGATCCGTCGAAGATCAAAAAAGTCGTACAGCTTATTTTGCAACATAAAAATAAAAATATTCTGATTGTTATTGGAAAGGGTGCGGTAAAAAACTCACACGAAATCCGCAGACTAGTAAATACGTTCAATCTACCATTTTTACCCACCCCAATGGCTAAGGGAATTGTCCCAGACTCTTCTCCACTGAACGTTTCATCTGCAAGATCTCAAGCATTGAAAATAGCGGATATTGTTCTTGTTCTCGGGGCAAGATTAAACTGGATATTGCATTTTGGTACTTCACCCAAATGGAACTCAGAATCCATATTCATCCAGTTCGATTCCAATCCCGAAACCTTGGGGGATAACAATGTCTCACCTGGTGCTGACCTTTCCATATGGGGAGATATAGGCTTGAGCGTAACCGCTTTAGTTGAGGAATTGACTCGCCAAGATTCCTGCTGGAAGTATAGTGGTGTTAAGCAAGAAATACGAGAGAAAATTCAACTGAATCAAACTCGTCTATTGAGGAAAGAGAAGACTAGAGGGGCACAATTGAATTACAACCAGGTTTATGGAACATTGAGGCCTCTTATCGATGATTACAGGACAATACTTGTGACGGAGGGAGCAAATACTATGGATATTGCGCGTATTTCATTCCCTACAGACGCTCCAAGGCGCCGTTTAGACGCAGGGACCAACGCGACTATGGGGATTGGGCTCGGATATGCTCTTGCATGCAAGGCATCTCATCCGGAACTCGATGTGGTGCTGATTCAGGGTGATTCCGCATTTGGATTCTCTGCCATGGAAATTGAAACGGCAGTAAGGTGTCAGCTGGCATTGGTCATCGTTGTGATGAACAATAGCGGTATTTACCATGGAGAAAAGGATATAGAGGGTGATTTACCTCCAACGGCACTAAGCAAGAACTGCCGTTACGATCTCGTAGGCAAAGGACTGGGTGCCAATGATTTTTTTGTCAACACAATAAGCGAACTGAGCAGATGCTTCCAACAAGCCGTGCAGCTTTCTCGGACTAAAAGAGAAACAAGTGTCATCAACGTTATCATTGAGCCTGGCGAACAAAAGCAAATTGCCTTTGCCTGGCAGAATAAACCGCGTTTA
SEQ ID NO:123(核糖体结合位点RBS4和Sc_YEL020C开放读码框)：
GCATCAACACCTAAATTAATTAACACACCATATATTAAACCATAAAAAATAAGAAAATGACCACGACCGCTACCCAACACTTCGCTCAACTTTTACAAAAATATGGCATCGACACAGTCTTTGGAATTGTGGGCATCCCTATTGTCCAACTAGCGGATACGATGGTTGCCAACGGTATCAAATTCATTCCATGCAGGAACGAACAGGCTGCCTCATATGCAGCCTCTGCTTATGGTTATATTAGTGATAAGCCCGGCGTGTTACTTATTGTCGGGGGCCCCGGTTTAATCCACGCACTGGCTGGTATATACAACTCAATGAGTAATAGGTGGCCTCTTCTAGTAATTGCAGGGAGTTCTTCTCAAAGTGATATACATAAAGGCGGGTTTCAAGAGTTGGATCAAGTTAGTCTTTTGTCCCCATTTTTGAAGTTTACCGGAAAGTTAACCCCTGACAATATTGACATGATTACTCAGAAGGCTTTGAATTACTGTATACAAGGCACTGCAGGGGTTTCTTACATAGACGTCCCTGCAGATTTTATTGAGTATGAAAAACCCTTAGAAGGAAACGACCGTACAGGAAATGAATTACCGATGATATTAACTCCAAACATATGTGGCCCTGATCCGTCGAAGATCAAAAAAGTCGTACAGCTTATTTTGCAACATAAAAATAAAAATATTCTGATTGTTATTGGAAAGGGTGCGGTAAAAAACTCACACGAAATCCGCAGACTAGTAAATACGTTCAATCTACCATTTTTACCCACCCCAATGGCTAAGGGAATTGTCCCAGACTCTTCTCCACTGAACGTTTCATCTGCAAGATCTCAAGCATTGAAAATAGCGGATATTGTTCTTGTTCTCGGGGCAAGATTAAACTGGATATTGCATTTTGGTACTTCACCCAAATGGAACTCAGAATCCATATTCATCCAGTTCGATTCCAATCCCGAAACCTTGGGGGATAACAATGTCTCACCTGGTGCTGACCTTTCCATATGGGGAGATATAGGCTTGAGCGTAACCGCTTTAGTTGAGGAATTGACTCGCCAAGATTCCTGCTGGAAGTATAGTGGTGTTAAGCAAGAAATACGAGAGAAAATTCAACTGAATCAAACTCGTCTATTGAGGAAAGAGAAGACTAGAGGGGCACAATTGAATTACAACCAGGTTTATGGAACATTGAGGCCTCTTATCGATGATTACAGGACAATACTTGTGACGGAGGGAGCAAATACTATGGATATTGCGCGTATTTCATTCCCTACAGACGCTCCAAGGCGCCGTTTAGACGCAGGGACCAACGCGACTATGGGGATTGGGCTCGGATATGCTCTTGCATGCAAGGCATCTCATCCGGAACTCGATGTGGTGCTGATTCAGGGTGATTCCGCATTTGGATTCTCTGCCATGGAAATTGAAACGGCAGTAAGGTGTCAGCTGGCATTGGTCATCGTTGTGATGAACAATAGCGGTATTTACCATGGAGAAAAGGATATAGAGGGTGATTTACCTCCAACGGCACTAAGCAAGAACTGCCGTTACGATCTCGTAGGCAAAGGACTGGGTGCCAATGATTTTTTTGTCAACACAATAAGCGAACTGAGCAGATGCTTCCAACAAGCCGTGCAGCTTTCTCGGACTAAAAGAGAAACAAGTGTCATCAACGTTATCATTGAGCCTGGCGAACAAAAGCAAATTGCCTTTGCCTGGCAGAATAAACCGCGTTTA
SEQ ID NO:124(核糖体结合位点RBS5和Sc_YEL020C开放读码框)：
GCATCTTCTACCCATATTCCCCCTAATCCCATCTGTTATATTATTAAATTTAATAAAATGACCACGACCGCTACCCAACACTTCGCTCAACTTTTACAAAAATATGGCATCGACACAGTCTTTGGAATTGTGGGCATCCCTATTGTCCAACTAGCGGATACGATGGTTGCCAACGGTATCAAATTCATTCCATGCAGGAACGAACAGGCTGCCTCATATGCAGCCTCTGCTTATGGTTATATTAGTGATAAGCCCGGCGTGTTACTTATTGTCGGGGGCCCCGGTTTAATCCACGCACTGGCTGGTATATACAACTCAATGAGTAATAGGTGGCCTCTTCTAGTAATTGCAGGGAGTTCTTCTCAAAGTGATATACATAAAGGCGGGTTTCAAGAGTTGGATCAAGTTAGTCTTTTGTCCCCATTTTTGAAGTTTACCGGAAAGTTAACCCCTGACAATATTGACATGATTACTCAGAAGGCTTTGAATTACTGTATACAAGGCACTGCAGGGGTTTCTTACATAGACGTCCCTGCAGATTTTATTGAGTATGAAAAACCCTTAGAAGGAAACGACCGTACAGGAAATGAATTACCGATGATATTAACTCCAAACATATGTGGCCCTGATCCGTCGAAGATCAAAAAAGTCGTACAGCTTATTTTGCAACATAAAAATAAAAATATTCTGATTGTTATTGGAAAGGGTGCGGTAAAAAACTCACACGAAATCCGCAGACTAGTAAATACGTTCAATCTACCATTTTTACCCACCCCAATGGCTAAGGGAATTGTCCCAGACTCTTCTCCACTGAACGTTTCATCTGCAAGATCTCAAGCATTGAAAATAGCGGATATTGTTCTTGTTCTCGGGGCAAGATTAAACTGGATATTGCATTTTGGTACTTCACCCAAATGGAACTCAGAATCCATATTCATCCAGTTCGATTCCAATCCCGAAACCTTGGGGGATAACAATGTCTCACCTGGTGCTGACCTTTCCATATGGGGAGATATAGGCTTGAGCGTAACCGCTTTAGTTGAGGAATTGACTCGCCAAGATTCCTGCTGGAAGTATAGTGGTGTTAAGCAAGAAATACGAGAGAAAATTCAACTGAATCAAACTCGTCTATTGAGGAAAGAGAAGACTAGAGGGGCACAATTGAATTACAACCAGGTTTATGGAACATTGAGGCCTCTTATCGATGATTACAGGACAATACTTGTGACGGAGGGAGCAAATACTATGGATATTGCGCGTATTTCATTCCCTACAGACGCTCCAAGGCGCCGTTTAGACGCAGGGACCAACGCGACTATGGGGATTGGGCTCGGATATGCTCTTGCATGCAAGGCATCTCATCCGGAACTCGATGTGGTGCTGATTCAGGGTGATTCCGCATTTGGATTCTCTGCCATGGAAATTGAAACGGCAGTAAGGTGTCAGCTGGCATTGGTCATCGTTGTGATGAACAATAGCGGTATTTACCATGGAGAAAAGGATATAGAGGGTGATTTACCTCCAACGGCACTAAGCAAGAACTGCCGTTACGATCTCGTAGGCAAAGGACTGGGTGCCAATGATTTTTTTGTCAACACAATAAGCGAACTGAGCAGATGCTTCCAACAAGCCGTGCAGCTTTCTCGGACTAAAAGAGAAACAAGTGTCATCAACGTTATCATTGAGCCTGGCGAACAAAAGCAAATTGCCTTTGCCTGGCAGAATAAACCGCGTTTA
SEQ ID NO:125(核糖体结合位点RBS6和Sc_YEL020C开放读码框)：
GCATCATCTACCTACATTATTTAACAAACCACATTTAAAAGGAAAAAATATTAAAAAATGACCACGACCGCTACCCAACACTTCGCTCAACTTTTACAAAAATATGGCATCGACACAGTCTTTGGAATTGTGGGCATCCCTATTGTCCAACTAGCGGATACGATGGTTGCCAACGGTATCAAATTCATTCCATGCAGGAACGAACAGGCTGCCTCATATGCAGCCTCTGCTTATGGTTATATTAGTGATAAGCCCGGCGTGTTACTTATTGTCGGGGGCCCCGGTTTAATCCACGCACTGGCTGGTATATACAACTCAATGAGTAATAGGTGGCCTCTTCTAGTAATTGCAGGGAGTTCTTCTCAAAGTGATATACATAAAGGCGGGTTTCAAGAGTTGGATCAAGTTAGTCTTTTGTCCCCATTTTTGAAGTTTACCGGAAAGTTAACCCCTGACAATATTGACATGATTACTCAGAAGGCTTTGAATTACTGTATACAAGGCACTGCAGGGGTTTCTTACATAGACGTCCCTGCAGATTTTATTGAGTATGAAAAACCCTTAGAAGGAAACGACCGTACAGGAAATGAATTACCGATGATATTAACTCCAAACATATGTGGCCCTGATCCGTCGAAGATCAAAAAAGTCGTACAGCTTATTTTGCAACATAAAAATAAAAATATTCTGATTGTTATTGGAAAGGGTGCGGTAAAAAACTCACACGAAATCCGCAGACTAGTAAATACGTTCAATCTACCATTTTTACCCACCCCAATGGCTAAGGGAATTGTCCCAGACTCTTCTCCACTGAACGTTTCATCTGCAAGATCTCAAGCATTGAAAATAGCGGATATTGTTCTTGTTCTCGGGGCAAGATTAAACTGGATATTGCATTTTGGTACTTCACCCAAATGGAACTCAGAATCCATATTCATCCAGTTCGATTCCAATCCCGAAACCTTGGGGGATAACAATGTCTCACCTGGTGCTGACCTTTCCATATGGGGAGATATAGGCTTGAGCGTAACCGCTTTAGTTGAGGAATTGACTCGCCAAGATTCCTGCTGGAAGTATAGTGGTGTTAAGCAAGAAATACGAGAGAAAATTCAACTGAATCAAACTCGTCTATTGAGGAAAGAGAAGACTAGAGGGGCACAATTGAATTACAACCAGGTTTATGGAACATTGAGGCCTCTTATCGATGATTACAGGACAATACTTGTGACGGAGGGAGCAAATACTATGGATATTGCGCGTATTTCATTCCCTACAGACGCTCCAAGGCGCCGTTTAGACGCAGGGACCAACGCGACTATGGGGATTGGGCTCGGATATGCTCTTGCATGCAAGGCATCTCATCCGGAACTCGATGTGGTGCTGATTCAGGGTGATTCCGCATTTGGATTCTCTGCCATGGAAATTGAAACGGCAGTAAGGTGTCAGCTGGCATTGGTCATCGTTGTGATGAACAATAGCGGTATTTACCATGGAGAAAAGGATATAGAGGGTGATTTACCTCCAACGGCACTAAGCAAGAACTGCCGTTACGATCTCGTAGGCAAAGGACTGGGTGCCAATGATTTTTTTGTCAACACAATAAGCGAACTGAGCAGATGCTTCCAACAAGCCGTGCAGCTTTCTCGGACTAAAAGAGAAACAAGTGTCATCAACGTTATCATTGAGCCTGGCGAACAAAAGCAAATTGCCTTTGCCTGGCAGAATAAACCGCGTTTA
SEQ ID NO:126(核糖体结合位点RBS7和Sc_YEL020C开放读码框)：
GCATCACCTACCTATATTATTTATCATTCCACATGCGTTATTATTAAATTTTAAAAAATGACCACGACCGCTACCCAACACTTCGCTCAACTTTTACAAAAATATGGCATCGACACAGTCTTTGGAATTGTGGGCATCCCTATTGTCCAACTAGCGGATACGATGGTTGCCAACGGTATCAAATTCATTCCATGCAGGAACGAACAGGCTGCCTCATATGCAGCCTCTGCTTATGGTTATATTAGTGATAAGCCCGGCGTGTTACTTATTGTCGGGGGCCCCGGTTTAATCCACGCACTGGCTGGTATATACAACTCAATGAGTAATAGGTGGCCTCTTCTAGTAATTGCAGGGAGTTCTTCTCAAAGTGATATACATAAAGGCGGGTTTCAAGAGTTGGATCAAGTTAGTCTTTTGTCCCCATTTTTGAAGTTTACCGGAAAGTTAACCCCTGACAATATTGACATGATTACTCAGAAGGCTTTGAATTACTGTATACAAGGCACTGCAGGGGTTTCTTACATAGACGTCCCTGCAGATTTTATTGAGTATGAAAAACCCTTAGAAGGAAACGACCGTACAGGAAATGAATTACCGATGATATTAACTCCAAACATATGTGGCCCTGATCCGTCGAAGATCAAAAAAGTCGTACAGCTTATTTTGCAACATAAAAATAAAAATATTCTGATTGTTATTGGAAAGGGTGCGGTAAAAAACTCACACGAAATCCGCAGACTAGTAAATACGTTCAATCTACCATTTTTACCCACCCCAATGGCTAAGGGAATTGTCCCAGACTCTTCTCCACTGAACGTTTCATCTGCAAGATCTCAAGCATTGAAAATAGCGGATATTGTTCTTGTTCTCGGGGCAAGATTAAACTGGATATTGCATTTTGGTACTTCACCCAAATGGAACTCAGAATCCATATTCATCCAGTTCGATTCCAATCCCGAAACCTTGGGGGATAACAATGTCTCACCTGGTGCTGACCTTTCCATATGGGGAGATATAGGCTTGAGCGTAACCGCTTTAGTTGAGGAATTGACTCGCCAAGATTCCTGCTGGAAGTATAGTGGTGTTAAGCAAGAAATACGAGAGAAAATTCAACTGAATCAAACTCGTCTATTGAGGAAAGAGAAGACTAGAGGGGCACAATTGAATTACAACCAGGTTTATGGAACATTGAGGCCTCTTATCGATGATTACAGGACAATACTTGTGACGGAGGGAGCAAATACTATGGATATTGCGCGTATTTCATTCCCTACAGACGCTCCAAGGCGCCGTTTAGACGCAGGGACCAACGCGACTATGGGGATTGGGCTCGGATATGCTCTTGCATGCAAGGCATCTCATCCGGAACTCGATGTGGTGCTGATTCAGGGTGATTCCGCATTTGGATTCTCTGCCATGGAAATTGAAACGGCAGTAAGGTGTCAGCTGGCATTGGTCATCGTTGTGATGAACAATAGCGGTATTTACCATGGAGAAAAGGATATAGAGGGTGATTTACCTCCAACGGCACTAAGCAAGAACTGCCGTTACGATCTCGTAGGCAAAGGACTGGGTGCCAATGATTTTTTTGTCAACACAATAAGCGAACTGAGCAGATGCTTCCAACAAGCCGTGCAGCTTTCTCGGACTAAAAGAGAAACAAGTGTCATCAACGTTATCATTGAGCCTGGCGAACAAAAGCAAATTGCCTTTGCCTGGCAGAATAAACCGCGTTTA
SEQ ID NO:127(核糖体结合位点RBS8和Sc_YEL020C开放读码框)：
GCATCTAAACAACATATTCCCCCTAAACCAAAATATTTAGGGTATAAAAATTAAAAAATGACCACGACCGCTACCCAACACTTCGCTCAACTTTTACAAAAATATGGCATCGACACAGTCTTTGGAATTGTGGGCATCCCTATTGTCCAACTAGCGGATACGATGGTTGCCAACGGTATCAAATTCATTCCATGCAGGAACGAACAGGCTGCCTCATATGCAGCCTCTGCTTATGGTTATATTAGTGATAAGCCCGGCGTGTTACTTATTGTCGGGGGCCCCGGTTTAATCCACGCACTGGCTGGTATATACAACTCAATGAGTAATAGGTGGCCTCTTCTAGTAATTGCAGGGAGTTCTTCTCAAAGTGATATACATAAAGGCGGGTTTCAAGAGTTGGATCAAGTTAGTCTTTTGTCCCCATTTTTGAAGTTTACCGGAAAGTTAACCCCTGACAATATTGACATGATTACTCAGAAGGCTTTGAATTACTGTATACAAGGCACTGCAGGGGTTTCTTACATAGACGTCCCTGCAGATTTTATTGAGTATGAAAAACCCTTAGAAGGAAACGACCGTACAGGAAATGAATTACCGATGATATTAACTCCAAACATATGTGGCCCTGATCCGTCGAAGATCAAAAAAGTCGTACAGCTTATTTTGCAACATAAAAATAAAAATATTCTGATTGTTATTGGAAAGGGTGCGGTAAAAAACTCACACGAAATCCGCAGACTAGTAAATACGTTCAATCTACCATTTTTACCCACCCCAATGGCTAAGGGAATTGTCCCAGACTCTTCTCCACTGAACGTTTCATCTGCAAGATCTCAAGCATTGAAAATAGCGGATATTGTTCTTGTTCTCGGGGCAAGATTAAACTGGATATTGCATTTTGGTACTTCACCCAAATGGAACTCAGAATCCATATTCATCCAGTTCGATTCCAATCCCGAAACCTTGGGGGATAACAATGTCTCACCTGGTGCTGACCTTTCCATATGGGGAGATATAGGCTTGAGCGTAACCGCTTTAGTTGAGGAATTGACTCGCCAAGATTCCTGCTGGAAGTATAGTGGTGTTAAGCAAGAAATACGAGAGAAAATTCAACTGAATCAAACTCGTCTATTGAGGAAAGAGAAGACTAGAGGGGCACAATTGAATTACAACCAGGTTTATGGAACATTGAGGCCTCTTATCGATGATTACAGGACAATACTTGTGACGGAGGGAGCAAATACTATGGATATTGCGCGTATTTCATTCCCTACAGACGCTCCAAGGCGCCGTTTAGACGCAGGGACCAACGCGACTATGGGGATTGGGCTCGGATATGCTCTTGCATGCAAGGCATCTCATCCGGAACTCGATGTGGTGCTGATTCAGGGTGATTCCGCATTTGGATTCTCTGCCATGGAAATTGAAACGGCAGTAAGGTGTCAGCTGGCATTGGTCATCGTTGTGATGAACAATAGCGGTATTTACCATGGAGAAAAGGATATAGAGGGTGATTTACCTCCAACGGCACTAAGCAAGAACTGCCGTTACGATCTCGTAGGCAAAGGACTGGGTGCCAATGATTTTTTTGTCAACACAATAAGCGAACTGAGCAGATGCTTCCAACAAGCCGTGCAGCTTTCTCGGACTAAAAGAGAAACAAGTGTCATCAACGTTATCATTGAGCCTGGCGAACAAAAGCAAATTGCCTTTGCCTGGCAGAATAAACCGCGTTTA
SEQ ID NO:128(核糖体结合位点RBS1和Ec_yfdE开放读码框)：
GCATCATCTACCTTTATTAATCAACAATCCAAATTATTAATAAAAAAAATTTAAAAAATGACAAATAATGAAAGCAAAGGGCCGTTTGAAGGCTTATTAGTTATCGATATGACACATGTCCTTAATGGACCTTTCGGAACTCAACTTCTTTGTAATATGGGCGCAAGGGTAATTAAAGTTGAGCCGCCGGGTCATGGTGATGATACCCGCACATTTGGTCCCTATGTGGATGGACAGTCACTCTATTACAGTTTTATTAATCATGGCAAAGAGAGTGTGGTTCTTGATTTAAAGAATGATCACGATAAAAGTATATTTATAAATATGCTCAAACAAGCTGATGTATTAGCTGAGAATTTTCGCCCAGGTACAATGGAAAAACTGGGGTTTTCATGGGAAACGCTTCAAGAAATCAACCCGCGCCTCATATATGCTTCATCGTCAGGTTTCGGACATACCGGTCCGCTAAAAGATGCTCCTGCCTACGATACCATCATTCAGGCAATGAGCGGGATAATGATGGAAACAGGATATCCTGATGCTCCGCCAGTGCGCGTTGGTACATCTCTTGCGGATCTATGCGGCGGTGTCTATTTATTCAGCGGAATAGTGAGTGCACTTTATGGCCGCGAAAAGAGCCAGAGAGGGGCGCATGTCGATATAGCGATGTTTGATGCCACGCTGAGTTTTCTGGAGCATGGTCTGATGGCATATATCGCAACTGGGAAGTCACCACAACGTCTGGGAAATCGCCATCCCTACATGGCACCTTTTGATGTTTTCAATACTCAGGATAAGCCGATTACGATTTGTTGTGGTAATGACAAGCTTTTTTCTGCGTTATGCCAGGCACTGGAGCTTACGGAACTGGTTAATGATCCCCGATTTAGCAGCAATATTTTACGCGTACAAAACCAGGCTATTCTTAAACAATATATTGAGCGGACGTTAAAAACGCAGGCAGCTGAAGTTTGGTTAGCCAGAATACATGAAGTTGGTGTACCCGTCGCGCCGTTATTAAGTGTGGCTGAGGCCATTAAATTGCCACAAACTCAGGCGAGAAATATGTTGATTGAAGCCGGGGGAATAATGATGCCGGGTAATCCGATAAAAATCAGCGGCTGCGCGGACCCGCATGTTATGCCGGGAGCGGCAACGCTCGACCAGCATGGGGAACAAATTCGCCAGGAGTTCTCATCA
SEQ ID NO:129(核糖体结合位点RBS2和Ec_yfdE开放读码框)：
GCATCAACTACCTAAATTACTTATCAAACCACTTAGTAGTAAAAAAAATATAACAAAATGACAAATAATGAAAGCAAAGGGCCGTTTGAAGGCTTATTAGTTATCGATATGACACATGTCCTTAATGGACCTTTCGGAACTCAACTTCTTTGTAATATGGGCGCAAGGGTAATTAAAGTTGAGCCGCCGGGTCATGGTGATGATACCCGCACATTTGGTCCCTATGTGGATGGACAGTCACTCTATTACAGTTTTATTAATCATGGCAAAGAGAGTGTGGTTCTTGATTTAAAGAATGATCACGATAAAAGTATATTTATAAATATGCTCAAACAAGCTGATGTATTAGCTGAGAATTTTCGCCCAGGTACAATGGAAAAACTGGGGTTTTCATGGGAAACGCTTCAAGAAATCAACCCGCGCCTCATATATGCTTCATCGTCAGGTTTCGGACATACCGGTCCGCTAAAAGATGCTCCTGCCTACGATACCATCATTCAGGCAATGAGCGGGATAATGATGGAAACAGGATATCCTGATGCTCCGCCAGTGCGCGTTGGTACATCTCTTGCGGATCTATGCGGCGGTGTCTATTTATTCAGCGGAATAGTGAGTGCACTTTATGGCCGCGAAAAGAGCCAGAGAGGGGCGCATGTCGATATAGCGATGTTTGATGCCACGCTGAGTTTTCTGGAGCATGGTCTGATGGCATATATCGCAACTGGGAAGTCACCACAACGTCTGGGAAATCGCCATCCCTACATGGCACCTTTTGATGTTTTCAATACTCAGGATAAGCCGATTACGATTTGTTGTGGTAATGACAAGCTTTTTTCTGCGTTATGCCAGGCACTGGAGCTTACGGAACTGGTTAATGATCCCCGATTTAGCAGCAATATTTTACGCGTACAAAACCAGGCTATTCTTAAACAATATATTGAGCGGACGTTAAAAACGCAGGCAGCTGAAGTTTGGTTAGCCAGAATACATGAAGTTGGTGTACCCGTCGCGCCGTTATTAAGTGTGGCTGAGGCCATTAAATTGCCACAAACTCAGGCGAGAAATATGTTGATTGAAGCCGGGGGAATAATGATGCCGGGTAATCCGATAAAAATCAGCGGCTGCGCGGACCCGCATGTTATGCCGGGAGCGGCAACGCTCGACCAGCATGGGGAACAAATTCGCCAGGAGTTCTCATCA
SEQ ID NO:130(核糖体结合位点RBS3和Ec_yfdE开放读码框)：
GCATCACTATTACAAAAAAAACACCAATATTACATGGTCTCTTTTAAATATTATAAAATGACAAATAATGAAAGCAAAGGGCCGTTTGAAGGCTTATTAGTTATCGATATGACACATGTCCTTAATGGACCTTTCGGAACTCAACTTCTTTGTAATATGGGCGCAAGGGTAATTAAAGTTGAGCCGCCGGGTCATGGTGATGATACCCGCACATTTGGTCCCTATGTGGATGGACAGTCACTCTATTACAGTTTTATTAATCATGGCAAAGAGAGTGTGGTTCTTGATTTAAAGAATGATCACGATAAAAGTATATTTATAAATATGCTCAAACAAGCTGATGTATTAGCTGAGAATTTTCGCCCAGGTACAATGGAAAAACTGGGGTTTTCATGGGAAACGCTTCAAGAAATCAACCCGCGCCTCATATATGCTTCATCGTCAGGTTTCGGACATACCGGTCCGCTAAAAGATGCTCCTGCCTACGATACCATCATTCAGGCAATGAGCGGGATAATGATGGAAACAGGATATCCTGATGCTCCGCCAGTGCGCGTTGGTACATCTCTTGCGGATCTATGCGGCGGTGTCTATTTATTCAGCGGAATAGTGAGTGCACTTTATGGCCGCGAAAAGAGCCAGAGAGGGGCGCATGTCGATATAGCGATGTTTGATGCCACGCTGAGTTTTCTGGAGCATGGTCTGATGGCATATATCGCAACTGGGAAGTCACCACAACGTCTGGGAAATCGCCATCCCTACATGGCACCTTTTGATGTTTTCAATACTCAGGATAAGCCGATTACGATTTGTTGTGGTAATGACAAGCTTTTTTCTGCGTTATGCCAGGCACTGGAGCTTACGGAACTGGTTAATGATCCCCGATTTAGCAGCAATATTTTACGCGTACAAAACCAGGCTATTCTTAAACAATATATTGAGCGGACGTTAAAAACGCAGGCAGCTGAAGTTTGGTTAGCCAGAATACATGAAGTTGGTGTACCCGTCGCGCCGTTATTAAGTGTGGCTGAGGCCATTAAATTGCCACAAACTCAGGCGAGAAATATGTTGATTGAAGCCGGGGGAATAATGATGCCGGGTAATCCGATAAAAATCAGCGGCTGCGCGGACCCGCATGTTATGCCGGGAGCGGCAACGCTCGACCAGCATGGGGAACAAATTCGCCAGGAGTTCTCATCA
SEQ ID NO:131(核糖体结合位点RBS4和Ec_yfdE开放读码框)：
GCATCATCTACCTATATTACTAAACAAACCAATTATAATAATTTTAAAAATAAAAAAATGACAAATAATGAAAGCAAAGGGCCGTTTGAAGGCTTATTAGTTATCGATATGACACATGTCCTTAATGGACCTTTCGGAACTCAACTTCTTTGTAATATGGGCGCAAGGGTAATTAAAGTTGAGCCGCCGGGTCATGGTGATGATACCCGCACATTTGGTCCCTATGTGGATGGACAGTCACTCTATTACAGTTTTATTAATCATGGCAAAGAGAGTGTGGTTCTTGATTTAAAGAATGATCACGATAAAAGTATATTTATAAATATGCTCAAACAAGCTGATGTATTAGCTGAGAATTTTCGCCCAGGTACAATGGAAAAACTGGGGTTTTCATGGGAAACGCTTCAAGAAATCAACCCGCGCCTCATATATGCTTCATCGTCAGGTTTCGGACATACCGGTCCGCTAAAAGATGCTCCTGCCTACGATACCATCATTCAGGCAATGAGCGGGATAATGATGGAAACAGGATATCCTGATGCTCCGCCAGTGCGCGTTGGTACATCTCTTGCGGATCTATGCGGCGGTGTCTATTTATTCAGCGGAATAGTGAGTGCACTTTATGGCCGCGAAAAGAGCCAGAGAGGGGCGCATGTCGATATAGCGATGTTTGATGCCACGCTGAGTTTTCTGGAGCATGGTCTGATGGCATATATCGCAACTGGGAAGTCACCACAACGTCTGGGAAATCGCCATCCCTACATGGCACCTTTTGATGTTTTCAATACTCAGGATAAGCCGATTACGATTTGTTGTGGTAATGACAAGCTTTTTTCTGCGTTATGCCAGGCACTGGAGCTTACGGAACTGGTTAATGATCCCCGATTTAGCAGCAATATTTTACGCGTACAAAACCAGGCTATTCTTAAACAATATATTGAGCGGACGTTAAAAACGCAGGCAGCTGAAGTTTGGTTAGCCAGAATACATGAAGTTGGTGTACCCGTCGCGCCGTTATTAAGTGTGGCTGAGGCCATTAAATTGCCACAAACTCAGGCGAGAAATATGTTGATTGAAGCCGGGGGAATAATGATGCCGGGTAATCCGATAAAAATCAGCGGCTGCGCGGACCCGCATGTTATGCCGGGAGCGGCAACGCTCGACCAGCATGGGGAACAAATTCGCCAGGAGTTCTCATCA
SEQ ID NO:132(核糖体结合位点RBS5和Ec_yfdE开放读码框)：
GCATCACCTACCTCTATTACTAATCAAACCATTTAAATGTAGTAAAAATATAATAAAATGACAAATAATGAAAGCAAAGGGCCGTTTGAAGGCTTATTAGTTATCGATATGACACATGTCCTTAATGGACCTTTCGGAACTCAACTTCTTTGTAATATGGGCGCAAGGGTAATTAAAGTTGAGCCGCCGGGTCATGGTGATGATACCCGCACATTTGGTCCCTATGTGGATGGACAGTCACTCTATTACAGTTTTATTAATCATGGCAAAGAGAGTGTGGTTCTTGATTTAAAGAATGATCACGATAAAAGTATATTTATAAATATGCTCAAACAAGCTGATGTATTAGCTGAGAATTTTCGCCCAGGTACAATGGAAAAACTGGGGTTTTCATGGGAAACGCTTCAAGAAATCAACCCGCGCCTCATATATGCTTCATCGTCAGGTTTCGGACATACCGGTCCGCTAAAAGATGCTCCTGCCTACGATACCATCATTCAGGCAATGAGCGGGATAATGATGGAAACAGGATATCCTGATGCTCCGCCAGTGCGCGTTGGTACATCTCTTGCGGATCTATGCGGCGGTGTCTATTTATTCAGCGGAATAGTGAGTGCACTTTATGGCCGCGAAAAGAGCCAGAGAGGGGCGCATGTCGATATAGCGATGTTTGATGCCACGCTGAGTTTTCTGGAGCATGGTCTGATGGCATATATCGCAACTGGGAAGTCACCACAACGTCTGGGAAATCGCCATCCCTACATGGCACCTTTTGATGTTTTCAATACTCAGGATAAGCCGATTACGATTTGTTGTGGTAATGACAAGCTTTTTTCTGCGTTATGCCAGGCACTGGAGCTTACGGAACTGGTTAATGATCCCCGATTTAGCAGCAATATTTTACGCGTACAAAACCAGGCTATTCTTAAACAATATATTGAGCGGACGTTAAAAACGCAGGCAGCTGAAGTTTGGTTAGCCAGAATACATGAAGTTGGTGTACCCGTCGCGCCGTTATTAAGTGTGGCTGAGGCCATTAAATTGCCACAAACTCAGGCGAGAAATATGTTGATTGAAGCCGGGGGAATAATGATGCCGGGTAATCCGATAAAAATCAGCGGCTGCGCGGACCCGCATGTTATGCCGGGAGCGGCAACGCTCGACCAGCATGGGGAACAAATTCGCCAGGAGTTCTCATCA
SEQ ID NO:133(核糖体结合位点RBS6和Ec_yfdE开放读码框)：
GCATCAACTACCTAAATTAATAAACACACCAACTTCCATGAATAAAAATATAAAAAAATGACAAATAATGAAAGCAAAGGGCCGTTTGAAGGCTTATTAGTTATCGATATGACACATGTCCTTAATGGACCTTTCGGAACTCAACTTCTTTGTAATATGGGCGCAAGGGTAATTAAAGTTGAGCCGCCGGGTCATGGTGATGATACCCGCACATTTGGTCCCTATGTGGATGGACAGTCACTCTATTACAGTTTTATTAATCATGGCAAAGAGAGTGTGGTTCTTGATTTAAAGAATGATCACGATAAAAGTATATTTATAAATATGCTCAAACAAGCTGATGTATTAGCTGAGAATTTTCGCCCAGGTACAATGGAAAAACTGGGGTTTTCATGGGAAACGCTTCAAGAAATCAACCCGCGCCTCATATATGCTTCATCGTCAGGTTTCGGACATACCGGTCCGCTAAAAGATGCTCCTGCCTACGATACCATCATTCAGGCAATGAGCGGGATAATGATGGAAACAGGATATCCTGATGCTCCGCCAGTGCGCGTTGGTACATCTCTTGCGGATCTATGCGGCGGTGTCTATTTATTCAGCGGAATAGTGAGTGCACTTTATGGCCGCGAAAAGAGCCAGAGAGGGGCGCATGTCGATATAGCGATGTTTGATGCCACGCTGAGTTTTCTGGAGCATGGTCTGATGGCATATATCGCAACTGGGAAGTCACCACAACGTCTGGGAAATCGCCATCCCTACATGGCACCTTTTGATGTTTTCAATACTCAGGATAAGCCGATTACGATTTGTTGTGGTAATGACAAGCTTTTTTCTGCGTTATGCCAGGCACTGGAGCTTACGGAACTGGTTAATGATCCCCGATTTAGCAGCAATATTTTACGCGTACAAAACCAGGCTATTCTTAAACAATATATTGAGCGGACGTTAAAAACGCAGGCAGCTGAAGTTTGGTTAGCCAGAATACATGAAGTTGGTGTACCCGTCGCGCCGTTATTAAGTGTGGCTGAGGCCATTAAATTGCCACAAACTCAGGCGAGAAATATGTTGATTGAAGCCGGGGGAATAATGATGCCGGGTAATCCGATAAAAATCAGCGGCTGCGCGGACCCGCATGTTATGCCGGGAGCGGCAACGCTCGACCAGCATGGGGAACAAATTCGCCAGGAGTTCTCATCA
SEQ ID NO:134(核糖体结合位点RBS7和Ec_yfdE开放读码框)：
GCATCATCTACCTATATTATTTACCACCCCACCTAACTTTAGATTAAAATTTACAAAATGACAAATAATGAAAGCAAAGGGCCGTTTGAAGGCTTATTAGTTATCGATATGACACATGTCCTTAATGGACCTTTCGGAACTCAACTTCTTTGTAATATGGGCGCAAGGGTAATTAAAGTTGAGCCGCCGGGTCATGGTGATGATACCCGCACATTTGGTCCCTATGTGGATGGACAGTCACTCTATTACAGTTTTATTAATCATGGCAAAGAGAGTGTGGTTCTTGATTTAAAGAATGATCACGATAAAAGTATATTTATAAATATGCTCAAACAAGCTGATGTATTAGCTGAGAATTTTCGCCCAGGTACAATGGAAAAACTGGGGTTTTCATGGGAAACGCTTCAAGAAATCAACCCGCGCCTCATATATGCTTCATCGTCAGGTTTCGGACATACCGGTCCGCTAAAAGATGCTCCTGCCTACGATACCATCATTCAGGCAATGAGCGGGATAATGATGGAAACAGGATATCCTGATGCTCCGCCAGTGCGCGTTGGTACATCTCTTGCGGATCTATGCGGCGGTGTCTATTTATTCAGCGGAATAGTGAGTGCACTTTATGGCCGCGAAAAGAGCCAGAGAGGGGCGCATGTCGATATAGCGATGTTTGATGCCACGCTGAGTTTTCTGGAGCATGGTCTGATGGCATATATCGCAACTGGGAAGTCACCACAACGTCTGGGAAATCGCCATCCCTACATGGCACCTTTTGATGTTTTCAATACTCAGGATAAGCCGATTACGATTTGTTGTGGTAATGACAAGCTTTTTTCTGCGTTATGCCAGGCACTGGAGCTTACGGAACTGGTTAATGATCCCCGATTTAGCAGCAATATTTTACGCGTACAAAACCAGGCTATTCTTAAACAATATATTGAGCGGACGTTAAAAACGCAGGCAGCTGAAGTTTGGTTAGCCAGAATACATGAAGTTGGTGTACCCGTCGCGCCGTTATTAAGTGTGGCTGAGGCCATTAAATTGCCACAAACTCAGGCGAGAAATATGTTGATTGAAGCCGGGGGAATAATGATGCCGGGTAATCCGATAAAAATCAGCGGCTGCGCGGACCCGCATGTTATGCCGGGAGCGGCAACGCTCGACCAGCATGGGGAACAAATTCGCCAGGAGTTCTCATCA
SEQ ID NO:135(核糖体结合位点RBS8和Ec_yfdE开放读码框)：
GCATCACCTACCTTCATTACTCACCATACCAACTAGCACAGTTATAAAAATAAGAAAATGACAAATAATGAAAGCAAAGGGCCGTTTGAAGGCTTATTAGTTATCGATATGACACATGTCCTTAATGGACCTTTCGGAACTCAACTTCTTTGTAATATGGGCGCAAGGGTAATTAAAGTTGAGCCGCCGGGTCATGGTGATGATACCCGCACATTTGGTCCCTATGTGGATGGACAGTCACTCTATTACAGTTTTATTAATCATGGCAAAGAGAGTGTGGTTCTTGATTTAAAGAATGATCACGATAAAAGTATATTTATAAATATGCTCAAACAAGCTGATGTATTAGCTGAGAATTTTCGCCCAGGTACAATGGAAAAACTGGGGTTTTCATGGGAAACGCTTCAAGAAATCAACCCGCGCCTCATATATGCTTCATCGTCAGGTTTCGGACATACCGGTCCGCTAAAAGATGCTCCTGCCTACGATACCATCATTCAGGCAATGAGCGGGATAATGATGGAAACAGGATATCCTGATGCTCCGCCAGTGCGCGTTGGTACATCTCTTGCGGATCTATGCGGCGGTGTCTATTTATTCAGCGGAATAGTGAGTGCACTTTATGGCCGCGAAAAGAGCCAGAGAGGGGCGCATGTCGATATAGCGATGTTTGATGCCACGCTGAGTTTTCTGGAGCATGGTCTGATGGCATATATCGCAACTGGGAAGTCACCACAACGTCTGGGAAATCGCCATCCCTACATGGCACCTTTTGATGTTTTCAATACTCAGGATAAGCCGATTACGATTTGTTGTGGTAATGACAAGCTTTTTTCTGCGTTATGCCAGGCACTGGAGCTTACGGAACTGGTTAATGATCCCCGATTTAGCAGCAATATTTTACGCGTACAAAACCAGGCTATTCTTAAACAATATATTGAGCGGACGTTAAAAACGCAGGCAGCTGAAGTTTGGTTAGCCAGAATACATGAAGTTGGTGTACCCGTCGCGCCGTTATTAAGTGTGGCTGAGGCCATTAAATTGCCACAAACTCAGGCGAGAAATATGTTGATTGAAGCCGGGGGAATAATGATGCCGGGTAATCCGATAAAAATCAGCGGCTGCGCGGACCCGCATGTTATGCCGGGAGCGGCAACGCTCGACCAGCATGGGGAACAAATTCGCCAGGAGTTCTCATCA
SEQ ID NO:136(核糖体结合位点RBS1和Ec_frc开放读码框)：
GCATCATCTACCTAAATTAATAAACAACCCATTTATAAACCCTTTAAAATTAACAAAATGTCAACTCCACTTCAAGGAATTAAAGTTCTCGATTTCACCGGTGTGCAATCTGGCCCATCTTGTACTCAAATGCTGGCCTGGTTTGGCGCTGACGTTATTAAAATTGAACGTCCCGGCGTTGGTGACGTAACGCGTCACCAGCTGCGAGATATTCCTGATATCGATGCGCTTTACTTCACCATGCTTAACAGTAACAAACGTTCTATTGAGTTAAATACCAAAACAGCGGAAGGCAAAGAGGTAATGGAAAAGCTGATCCGCGAAGCTGATATCTTAGTCGAGAACTTTCATCCAGGGGCCATTGATCACATGGGCTTCACCTGGGAGCATATTCAAGAAATCAATCCACGTCTGATTTTTGGTTCGATCAAAGGGTTTGATGAGTGTTCGCCTTATGTGAATGTAAAAGCCTATGAAAACGTTGCTCAGGCAGCGGGTGGCGCGGCATCCACTACGGGTTTTTGGGATGGTCCGCCGCTGGTAAGCGCTGCAGCGTTGGGTGACAGCAACACCGGAATGCATTTGCTGATCGGTTTACTTGCTGCTTTGCTGCATCGCGAAAAAACGGGGCGTGGGCAACGAGTCACCATGTCAATGCAGGATGCCGTATTGAACCTTTGCCGCGTGAAATTACGTGACCAGCAGCGTCTAGATAAATTGGGTTATCTGGAAGAATACCCGCAGTATCCGAATGGTACATTTGGTGATGCAGTTCCCCGCGGTGGTAATGCAGGTGGTGGCGGTCAGCCTGGCTGGATCCTGAAATGTAAAGGCTGGGAAACCGATCCTAACGCCTATATTTATTTCACTATTCAGGAGCAAAACTGGGAAAACACCTGTAAAGCCATCGGCAAACCAGAATGGATTACCGATCCGGCATACAGTACAGCCCATGCACGACAGCCACATATTTTCGATATTTTTGCTGAAATCGAAAAATACACTGTCACTATTGATAAACATGAAGCGGTGGCCTATTTGACTCAGTTTGATATTCCTTGTGCACCGGTTTTAAGTATGAAAGAAATTTCACTTGATCCCTCTTTGCGCCAAAGTGGCAGTGTTGTTGAAGTGGAACAACCGTTGCGTGGAAAATATCTGACCGTTGGTTGTCCAATGAAATTCTCTGCCTTTACGCCGGATATTAAAGCTGCGCCGCTATTAGGTGAACATACCGCTGCTGTATTGCAGGAGCTGGGTTATAGCGACGATGAAATTGCTGCAATGAAGCAAAACCACGCCATC
SEQ ID NO:137(核糖体结合位点RBS2和Ec_frc开放读码框)：
GCATCTTAATCAATTTTAAACTCACCAAATCAAAATATGAAAAAAAAAAATAATAAAATGTCAACTCCACTTCAAGGAATTAAAGTTCTCGATTTCACCGGTGTGCAATCTGGCCCATCTTGTACTCAAATGCTGGCCTGGTTTGGCGCTGACGTTATTAAAATTGAACGTCCCGGCGTTGGTGACGTAACGCGTCACCAGCTGCGAGATATTCCTGATATCGATGCGCTTTACTTCACCATGCTTAACAGTAACAAACGTTCTATTGAGTTAAATACCAAAACAGCGGAAGGCAAAGAGGTAATGGAAAAGCTGATCCGCGAAGCTGATATCTTAGTCGAGAACTTTCATCCAGGGGCCATTGATCACATGGGCTTCACCTGGGAGCATATTCAAGAAATCAATCCACGTCTGATTTTTGGTTCGATCAAAGGGTTTGATGAGTGTTCGCCTTATGTGAATGTAAAAGCCTATGAAAACGTTGCTCAGGCAGCGGGTGGCGCGGCATCCACTACGGGTTTTTGGGATGGTCCGCCGCTGGTAAGCGCTGCAGCGTTGGGTGACAGCAACACCGGAATGCATTTGCTGATCGGTTTACTTGCTGCTTTGCTGCATCGCGAAAAAACGGGGCGTGGGCAACGAGTCACCATGTCAATGCAGGATGCCGTATTGAACCTTTGCCGCGTGAAATTACGTGACCAGCAGCGTCTAGATAAATTGGGTTATCTGGAAGAATACCCGCAGTATCCGAATGGTACATTTGGTGATGCAGTTCCCCGCGGTGGTAATGCAGGTGGTGGCGGTCAGCCTGGCTGGATCCTGAAATGTAAAGGCTGGGAAACCGATCCTAACGCCTATATTTATTTCACTATTCAGGAGCAAAACTGGGAAAACACCTGTAAAGCCATCGGCAAACCAGAATGGATTACCGATCCGGCATACAGTACAGCCCATGCACGACAGCCACATATTTTCGATATTTTTGCTGAAATCGAAAAATACACTGTCACTATTGATAAACATGAAGCGGTGGCCTATTTGACTCAGTTTGATATTCCTTGTGCACCGGTTTTAAGTATGAAAGAAATTTCACTTGATCCCTCTTTGCGCCAAAGTGGCAGTGTTGTTGAAGTGGAACAACCGTTGCGTGGAAAATATCTGACCGTTGGTTGTCCAATGAAATTCTCTGCCTTTACGCCGGATATTAAAGCTGCGCCGCTATTAGGTGAACATACCGCTGCTGTATTGCAGGAGCTGGGTTATAGCGACGATGAAATTGCTGCAATGAAGCAAAACCACGCCATC
SEQ ID NO:138(核糖体结合位点RBS3和Ec_frc开放读码框)：
GCATCATCTACCTTAATTAATAAACAAACCAATTTAACTAATTTAAAAAATATAAAATGTCAACTCCACTTCAAGGAATTAAAGTTCTCGATTTCACCGGTGTGCAATCTGGCCCATCTTGTACTCAAATGCTGGCCTGGTTTGGCGCTGACGTTATTAAAATTGAACGTCCCGGCGTTGGTGACGTAACGCGTCACCAGCTGCGAGATATTCCTGATATCGATGCGCTTTACTTCACCATGCTTAACAGTAACAAACGTTCTATTGAGTTAAATACCAAAACAGCGGAAGGCAAAGAGGTAATGGAAAAGCTGATCCGCGAAGCTGATATCTTAGTCGAGAACTTTCATCCAGGGGCCATTGATCACATGGGCTTCACCTGGGAGCATATTCAAGAAATCAATCCACGTCTGATTTTTGGTTCGATCAAAGGGTTTGATGAGTGTTCGCCTTATGTGAATGTAAAAGCCTATGAAAACGTTGCTCAGGCAGCGGGTGGCGCGGCATCCACTACGGGTTTTTGGGATGGTCCGCCGCTGGTAAGCGCTGCAGCGTTGGGTGACAGCAACACCGGAATGCATTTGCTGATCGGTTTACTTGCTGCTTTGCTGCATCGCGAAAAAACGGGGCGTGGGCAACGAGTCACCATGTCAATGCAGGATGCCGTATTGAACCTTTGCCGCGTGAAATTACGTGACCAGCAGCGTCTAGATAAATTGGGTTATCTGGAAGAATACCCGCAGTATCCGAATGGTACATTTGGTGATGCAGTTCCCCGCGGTGGTAATGCAGGTGGTGGCGGTCAGCCTGGCTGGATCCTGAAATGTAAAGGCTGGGAAACCGATCCTAACGCCTATATTTATTTCACTATTCAGGAGCAAAACTGGGAAAACACCTGTAAAGCCATCGGCAAACCAGAATGGATTACCGATCCGGCATACAGTACAGCCCATGCACGACAGCCACATATTTTCGATATTTTTGCTGAAATCGAAAAATACACTGTCACTATTGATAAACATGAAGCGGTGGCCTATTTGACTCAGTTTGATATTCCTTGTGCACCGGTTTTAAGTATGAAAGAAATTTCACTTGATCCCTCTTTGCGCCAAAGTGGCAGTGTTGTTGAAGTGGAACAACCGTTGCGTGGAAAATATCTGACCGTTGGTTGTCCAATGAAATTCTCTGCCTTTACGCCGGATATTAAAGCTGCGCCGCTATTAGGTGAACATACCGCTGCTGTATTGCAGGAGCTGGGTTATAGCGACGATGAAATTGCTGCAATGAAGCAAAACCACGCCATC
SEQ ID NO:139(核糖体结合位点RBS4和Ec_frc开放读码框)：
GCATCATCTACCTTCATTATTAATCATTCCAATTGTATCACTTAAAAATATAAAAAAATGTCAACTCCACTTCAAGGAATTAAAGTTCTCGATTTCACCGGTGTGCAATCTGGCCCATCTTGTACTCAAATGCTGGCCTGGTTTGGCGCTGACGTTATTAAAATTGAACGTCCCGGCGTTGGTGACGTAACGCGTCACCAGCTGCGAGATATTCCTGATATCGATGCGCTTTACTTCACCATGCTTAACAGTAACAAACGTTCTATTGAGTTAAATACCAAAACAGCGGAAGGCAAAGAGGTAATGGAAAAGCTGATCCGCGAAGCTGATATCTTAGTCGAGAACTTTCATCCAGGGGCCATTGATCACATGGGCTTCACCTGGGAGCATATTCAAGAAATCAATCCACGTCTGATTTTTGGTTCGATCAAAGGGTTTGATGAGTGTTCGCCTTATGTGAATGTAAAAGCCTATGAAAACGTTGCTCAGGCAGCGGGTGGCGCGGCATCCACTACGGGTTTTTGGGATGGTCCGCCGCTGGTAAGCGCTGCAGCGTTGGGTGACAGCAACACCGGAATGCATTTGCTGATCGGTTTACTTGCTGCTTTGCTGCATCGCGAAAAAACGGGGCGTGGGCAACGAGTCACCATGTCAATGCAGGATGCCGTATTGAACCTTTGCCGCGTGAAATTACGTGACCAGCAGCGTCTAGATAAATTGGGTTATCTGGAAGAATACCCGCAGTATCCGAATGGTACATTTGGTGATGCAGTTCCCCGCGGTGGTAATGCAGGTGGTGGCGGTCAGCCTGGCTGGATCCTGAAATGTAAAGGCTGGGAAACCGATCCTAACGCCTATATTTATTTCACTATTCAGGAGCAAAACTGGGAAAACACCTGTAAAGCCATCGGCAAACCAGAATGGATTACCGATCCGGCATACAGTACAGCCCATGCACGACAGCCACATATTTTCGATATTTTTGCTGAAATCGAAAAATACACTGTCACTATTGATAAACATGAAGCGGTGGCCTATTTGACTCAGTTTGATATTCCTTGTGCACCGGTTTTAAGTATGAAAGAAATTTCACTTGATCCCTCTTTGCGCCAAAGTGGCAGTGTTGTTGAAGTGGAACAACCGTTGCGTGGAAAATATCTGACCGTTGGTTGTCCAATGAAATTCTCTGCCTTTACGCCGGATATTAAAGCTGCGCCGCTATTAGGTGAACATACCGCTGCTGTATTGCAGGAGCTGGGTTATAGCGACGATGAAATTGCTGCAATGAAGCAAAACCACGCCATC
SEQ ID NO:140(核糖体结合位点RBS5和Ec_frc开放读码框)：
GCATCTTAATCAATTTTAAACTCACCAAATCAAATATCACGTAAAAAATATAAGAAAATGTCAACTCCACTTCAAGGAATTAAAGTTCTCGATTTCACCGGTGTGCAATCTGGCCCATCTTGTACTCAAATGCTGGCCTGGTTTGGCGCTGACGTTATTAAAATTGAACGTCCCGGCGTTGGTGACGTAACGCGTCACCAGCTGCGAGATATTCCTGATATCGATGCGCTTTACTTCACCATGCTTAACAGTAACAAACGTTCTATTGAGTTAAATACCAAAACAGCGGAAGGCAAAGAGGTAATGGAAAAGCTGATCCGCGAAGCTGATATCTTAGTCGAGAACTTTCATCCAGGGGCCATTGATCACATGGGCTTCACCTGGGAGCATATTCAAGAAATCAATCCACGTCTGATTTTTGGTTCGATCAAAGGGTTTGATGAGTGTTCGCCTTATGTGAATGTAAAAGCCTATGAAAACGTTGCTCAGGCAGCGGGTGGCGCGGCATCCACTACGGGTTTTTGGGATGGTCCGCCGCTGGTAAGCGCTGCAGCGTTGGGTGACAGCAACACCGGAATGCATTTGCTGATCGGTTTACTTGCTGCTTTGCTGCATCGCGAAAAAACGGGGCGTGGGCAACGAGTCACCATGTCAATGCAGGATGCCGTATTGAACCTTTGCCGCGTGAAATTACGTGACCAGCAGCGTCTAGATAAATTGGGTTATCTGGAAGAATACCCGCAGTATCCGAATGGTACATTTGGTGATGCAGTTCCCCGCGGTGGTAATGCAGGTGGTGGCGGTCAGCCTGGCTGGATCCTGAAATGTAAAGGCTGGGAAACCGATCCTAACGCCTATATTTATTTCACTATTCAGGAGCAAAACTGGGAAAACACCTGTAAAGCCATCGGCAAACCAGAATGGATTACCGATCCGGCATACAGTACAGCCCATGCACGACAGCCACATATTTTCGATATTTTTGCTGAAATCGAAAAATACACTGTCACTATTGATAAACATGAAGCGGTGGCCTATTTGACTCAGTTTGATATTCCTTGTGCACCGGTTTTAAGTATGAAAGAAATTTCACTTGATCCCTCTTTGCGCCAAAGTGGCAGTGTTGTTGAAGTGGAACAACCGTTGCGTGGAAAATATCTGACCGTTGGTTGTCCAATGAAATTCTCTGCCTTTACGCCGGATATTAAAGCTGCGCCGCTATTAGGTGAACATACCGCTGCTGTATTGCAGGAGCTGGGTTATAGCGACGATGAAATTGCTGCAATGAAGCAAAACCACGCCATC
SEQ ID NO:141(核糖体结合位点RBS6和Ec_frc开放读码框)：
GCATCAACTACCTCAATTATTTAACACACCAATTACTATACGTAAAAATATAAAAAAATGTCAACTCCACTTCAAGGAATTAAAGTTCTCGATTTCACCGGTGTGCAATCTGGCCCATCTTGTACTCAAATGCTGGCCTGGTTTGGCGCTGACGTTATTAAAATTGAACGTCCCGGCGTTGGTGACGTAACGCGTCACCAGCTGCGAGATATTCCTGATATCGATGCGCTTTACTTCACCATGCTTAACAGTAACAAACGTTCTATTGAGTTAAATACCAAAACAGCGGAAGGCAAAGAGGTAATGGAAAAGCTGATCCGCGAAGCTGATATCTTAGTCGAGAACTTTCATCCAGGGGCCATTGATCACATGGGCTTCACCTGGGAGCATATTCAAGAAATCAATCCACGTCTGATTTTTGGTTCGATCAAAGGGTTTGATGAGTGTTCGCCTTATGTGAATGTAAAAGCCTATGAAAACGTTGCTCAGGCAGCGGGTGGCGCGGCATCCACTACGGGTTTTTGGGATGGTCCGCCGCTGGTAAGCGCTGCAGCGTTGGGTGACAGCAACACCGGAATGCATTTGCTGATCGGTTTACTTGCTGCTTTGCTGCATCGCGAAAAAACGGGGCGTGGGCAACGAGTCACCATGTCAATGCAGGATGCCGTATTGAACCTTTGCCGCGTGAAATTACGTGACCAGCAGCGTCTAGATAAATTGGGTTATCTGGAAGAATACCCGCAGTATCCGAATGGTACATTTGGTGATGCAGTTCCCCGCGGTGGTAATGCAGGTGGTGGCGGTCAGCCTGGCTGGATCCTGAAATGTAAAGGCTGGGAAACCGATCCTAACGCCTATATTTATTTCACTATTCAGGAGCAAAACTGGGAAAACACCTGTAAAGCCATCGGCAAACCAGAATGGATTACCGATCCGGCATACAGTACAGCCCATGCACGACAGCCACATATTTTCGATATTTTTGCTGAAATCGAAAAATACACTGTCACTATTGATAAACATGAAGCGGTGGCCTATTTGACTCAGTTTGATATTCCTTGTGCACCGGTTTTAAGTATGAAAGAAATTTCACTTGATCCCTCTTTGCGCCAAAGTGGCAGTGTTGTTGAAGTGGAACAACCGTTGCGTGGAAAATATCTGACCGTTGGTTGTCCAATGAAATTCTCTGCCTTTACGCCGGATATTAAAGCTGCGCCGCTATTAGGTGAACATACCGCTGCTGTATTGCAGGAGCTGGGTTATAGCGACGATGAAATTGCTGCAATGAAGCAAAACCACGCCATC
SEQ ID NO:142(核糖体结合位点RBS7和Ec_frc开放读码框)：
GCATCTTAATCAATTTTAAACTCACCAAATCAAAAAAATGCGTATAAAAATTAAAAAATGTCAACTCCACTTCAAGGAATTAAAGTTCTCGATTTCACCGGTGTGCAATCTGGCCCATCTTGTACTCAAATGCTGGCCTGGTTTGGCGCTGACGTTATTAAAATTGAACGTCCCGGCGTTGGTGACGTAACGCGTCACCAGCTGCGAGATATTCCTGATATCGATGCGCTTTACTTCACCATGCTTAACAGTAACAAACGTTCTATTGAGTTAAATACCAAAACAGCGGAAGGCAAAGAGGTAATGGAAAAGCTGATCCGCGAAGCTGATATCTTAGTCGAGAACTTTCATCCAGGGGCCATTGATCACATGGGCTTCACCTGGGAGCATATTCAAGAAATCAATCCACGTCTGATTTTTGGTTCGATCAAAGGGTTTGATGAGTGTTCGCCTTATGTGAATGTAAAAGCCTATGAAAACGTTGCTCAGGCAGCGGGTGGCGCGGCATCCACTACGGGTTTTTGGGATGGTCCGCCGCTGGTAAGCGCTGCAGCGTTGGGTGACAGCAACACCGGAATGCATTTGCTGATCGGTTTACTTGCTGCTTTGCTGCATCGCGAAAAAACGGGGCGTGGGCAACGAGTCACCATGTCAATGCAGGATGCCGTATTGAACCTTTGCCGCGTGAAATTACGTGACCAGCAGCGTCTAGATAAATTGGGTTATCTGGAAGAATACCCGCAGTATCCGAATGGTACATTTGGTGATGCAGTTCCCCGCGGTGGTAATGCAGGTGGTGGCGGTCAGCCTGGCTGGATCCTGAAATGTAAAGGCTGGGAAACCGATCCTAACGCCTATATTTATTTCACTATTCAGGAGCAAAACTGGGAAAACACCTGTAAAGCCATCGGCAAACCAGAATGGATTACCGATCCGGCATACAGTACAGCCCATGCACGACAGCCACATATTTTCGATATTTTTGCTGAAATCGAAAAATACACTGTCACTATTGATAAACATGAAGCGGTGGCCTATTTGACTCAGTTTGATATTCCTTGTGCACCGGTTTTAAGTATGAAAGAAATTTCACTTGATCCCTCTTTGCGCCAAAGTGGCAGTGTTGTTGAAGTGGAACAACCGTTGCGTGGAAAATATCTGACCGTTGGTTGTCCAATGAAATTCTCTGCCTTTACGCCGGATATTAAAGCTGCGCCGCTATTAGGTGAACATACCGCTGCTGTATTGCAGGAGCTGGGTTATAGCGACGATGAAATTGCTGCAATGAAGCAAAACCACGCCATC
SEQ ID NO:143(核糖体结合位点RBS8和Ec_frc开放读码框)：
GCATCTTAATCAATTTTAAACTCACCAAATCAAAGGCCGACTTAAAAATATTAGAAAATGTCAACTCCACTTCAAGGAATTAAAGTTCTCGATTTCACCGGTGTGCAATCTGGCCCATCTTGTACTCAAATGCTGGCCTGGTTTGGCGCTGACGTTATTAAAATTGAACGTCCCGGCGTTGGTGACGTAACGCGTCACCAGCTGCGAGATATTCCTGATATCGATGCGCTTTACTTCACCATGCTTAACAGTAACAAACGTTCTATTGAGTTAAATACCAAAACAGCGGAAGGCAAAGAGGTAATGGAAAAGCTGATCCGCGAAGCTGATATCTTAGTCGAGAACTTTCATCCAGGGGCCATTGATCACATGGGCTTCACCTGGGAGCATATTCAAGAAATCAATCCACGTCTGATTTTTGGTTCGATCAAAGGGTTTGATGAGTGTTCGCCTTATGTGAATGTAAAAGCCTATGAAAACGTTGCTCAGGCAGCGGGTGGCGCGGCATCCACTACGGGTTTTTGGGATGGTCCGCCGCTGGTAAGCGCTGCAGCGTTGGGTGACAGCAACACCGGAATGCATTTGCTGATCGGTTTACTTGCTGCTTTGCTGCATCGCGAAAAAACGGGGCGTGGGCAACGAGTCACCATGTCAATGCAGGATGCCGTATTGAACCTTTGCCGCGTGAAATTACGTGACCAGCAGCGTCTAGATAAATTGGGTTATCTGGAAGAATACCCGCAGTATCCGAATGGTACATTTGGTGATGCAGTTCCCCGCGGTGGTAATGCAGGTGGTGGCGGTCAGCCTGGCTGGATCCTGAAATGTAAAGGCTGGGAAACCGATCCTAACGCCTATATTTATTTCACTATTCAGGAGCAAAACTGGGAAAACACCTGTAAAGCCATCGGCAAACCAGAATGGATTACCGATCCGGCATACAGTACAGCCCATGCACGACAGCCACATATTTTCGATATTTTTGCTGAAATCGAAAAATACACTGTCACTATTGATAAACATGAAGCGGTGGCCTATTTGACTCAGTTTGATATTCCTTGTGCACCGGTTTTAAGTATGAAAGAAATTTCACTTGATCCCTCTTTGCGCCAAAGTGGCAGTGTTGTTGAAGTGGAACAACCGTTGCGTGGAAAATATCTGACCGTTGGTTGTCCAATGAAATTCTCTGCCTTTACGCCGGATATTAAAGCTGCGCCGCTATTAGGTGAACATACCGCTGCTGTATTGCAGGAGCTGGGTTATAGCGACGATGAAATTGCTGCAATGAAGCAAAACCACGCCATC
SEQ ID NO:144(核糖体结合位点RBS1和Of_oxlT开放读码框)：
GCATCACCTAAAAATTATAAATAAATTAATAAACCTTTTAACATAAAAATTAATAAAATGAATAATCCACAAACAGGACAATCAACAGGCCTCTTGGGCAATCGTTGGTTCTACTTGGTATTAGCAGTTTTGCTGATGTGTATGATCTCGGGTGTCCAATATTCCTGGACACTGTACGCTAACCCGGTTAAAGACAACCTTGGCGTTTCTTTGGCTGCGGTTCAGACGGCTTTCACACTCTCTCAGGTCATTCAAGCTGGTTCTCAGCCTGGTGGTGGTTACTTCGTTGATAAATTCGGTCCAAGAATTCCATTGATGTTCGGTGGTGCGATGGTTCTCGCTGGCTGGACCTTCATGGGTATGGTTGACAGTGTTCCTGCTCTGTATGCTCTTTATACTCTGGCCGGTGCAGGTGTTGGTATCGTTTACGGTATCGCGATGAACACGGCTAACAGATGGTTCCCGGACAAACGCGGTCTGGCTTCCGGTTTCACCGCTGCCGGTTACGGTCTGGGTGTTCTGCCGTTCCTGCCACTGATCAGCTCCGTTCTGAAAGTTGAAGGTGTTGGCGCAGCATTCATGTACACCGGTTTGATCATGGGTATCCTGATTATCCTGATCGCTTTCGTTATCCGTTTCCCTGGCCAGCAAGGCGCCAAAAAACAAATCGTTGTTACCGACAAGGATTTCAATTCTGGCGAAATGCTGAGAACACCACAATTCTGGGTTCTGTGGACCGCATTCTTTTCCGTTAACTTTGGTGGTTTGCTGCTGGTTGCCAACAGCGTCCCTTACGGTCGCAGCCTCGGTCTTGCCGCAGGTGTGCTGACGATCGGTGTTTCGATCCAGAACCTGTTCAATGGTGGTTGCCGTCCTTTCTGGGGTTTCGTTTCCGATAAAATCGGCCGTTACAAAACCATGTCCGTCGTTTTCGGTATCAATGCTGTTGTTCTCGCACTTTTCCCGACGATTGCTGCCTTGGGCGATGTAGCCTTTATCGCCATGTTGGCAATCGCATTCTTCACATGGGGTGGTAGCTACGCTCTGTTCCCATCGACCAACAGCGATATTTTCGGTACGGCATACTCTGCCAGAAACTATGGTTTCTTCTGGGCTGCAAAAGCAACTGCCTCGATCTTCGGTGGTGGTCTGGGTGCTGCAATTGCAACCAACTTCGGATGGAATACCGCTTTCCTGATTACTGCGATTACTTCTTTCATCGCATTTGCTCTGGCTACCTTCGTTATTCCAAGAATGGGCCGTCCAGTCAAGAAAATGGTCAAATTGTCTCCAGAAGAAAAAGCTGTACAT
SEQ ID NO:145(核糖体结合位点RBS2和Of_oxlT开放读码框)：
GCATCACCTAAAAATTATAAATAAATTAATAAACTTTTATTAAAAAAAAATTACAAAATGAATAATCCACAAACAGGACAATCAACAGGCCTCTTGGGCAATCGTTGGTTCTACTTGGTATTAGCAGTTTTGCTGATGTGTATGATCTCGGGTGTCCAATATTCCTGGACACTGTACGCTAACCCGGTTAAAGACAACCTTGGCGTTTCTTTGGCTGCGGTTCAGACGGCTTTCACACTCTCTCAGGTCATTCAAGCTGGTTCTCAGCCTGGTGGTGGTTACTTCGTTGATAAATTCGGTCCAAGAATTCCATTGATGTTCGGTGGTGCGATGGTTCTCGCTGGCTGGACCTTCATGGGTATGGTTGACAGTGTTCCTGCTCTGTATGCTCTTTATACTCTGGCCGGTGCAGGTGTTGGTATCGTTTACGGTATCGCGATGAACACGGCTAACAGATGGTTCCCGGACAAACGCGGTCTGGCTTCCGGTTTCACCGCTGCCGGTTACGGTCTGGGTGTTCTGCCGTTCCTGCCACTGATCAGCTCCGTTCTGAAAGTTGAAGGTGTTGGCGCAGCATTCATGTACACCGGTTTGATCATGGGTATCCTGATTATCCTGATCGCTTTCGTTATCCGTTTCCCTGGCCAGCAAGGCGCCAAAAAACAAATCGTTGTTACCGACAAGGATTTCAATTCTGGCGAAATGCTGAGAACACCACAATTCTGGGTTCTGTGGACCGCATTCTTTTCCGTTAACTTTGGTGGTTTGCTGCTGGTTGCCAACAGCGTCCCTTACGGTCGCAGCCTCGGTCTTGCCGCAGGTGTGCTGACGATCGGTGTTTCGATCCAGAACCTGTTCAATGGTGGTTGCCGTCCTTTCTGGGGTTTCGTTTCCGATAAAATCGGCCGTTACAAAACCATGTCCGTCGTTTTCGGTATCAATGCTGTTGTTCTCGCACTTTTCCCGACGATTGCTGCCTTGGGCGATGTAGCCTTTATCGCCATGTTGGCAATCGCATTCTTCACATGGGGTGGTAGCTACGCTCTGTTCCCATCGACCAACAGCGATATTTTCGGTACGGCATACTCTGCCAGAAACTATGGTTTCTTCTGGGCTGCAAAAGCAACTGCCTCGATCTTCGGTGGTGGTCTGGGTGCTGCAATTGCAACCAACTTCGGATGGAATACCGCTTTCCTGATTACTGCGATTACTTCTTTCATCGCATTTGCTCTGGCTACCTTCGTTATTCCAAGAATGGGCCGTCCAGTCAAGAAAATGGTCAAATTGTCTCCAGAAGAAAAAGCTGTACAT
SEQ ID NO:146(核糖体结合位点RBS3和Of_oxlT开放读码框)：
GCATCACCTAAAAATTATAAATAAATTAATAAACATAACATAAATAAAATTAATAAAATGAATAATCCACAAACAGGACAATCAACAGGCCTCTTGGGCAATCGTTGGTTCTACTTGGTATTAGCAGTTTTGCTGATGTGTATGATCTCGGGTGTCCAATATTCCTGGACACTGTACGCTAACCCGGTTAAAGACAACCTTGGCGTTTCTTTGGCTGCGGTTCAGACGGCTTTCACACTCTCTCAGGTCATTCAAGCTGGTTCTCAGCCTGGTGGTGGTTACTTCGTTGATAAATTCGGTCCAAGAATTCCATTGATGTTCGGTGGTGCGATGGTTCTCGCTGGCTGGACCTTCATGGGTATGGTTGACAGTGTTCCTGCTCTGTATGCTCTTTATACTCTGGCCGGTGCAGGTGTTGGTATCGTTTACGGTATCGCGATGAACACGGCTAACAGATGGTTCCCGGACAAACGCGGTCTGGCTTCCGGTTTCACCGCTGCCGGTTACGGTCTGGGTGTTCTGCCGTTCCTGCCACTGATCAGCTCCGTTCTGAAAGTTGAAGGTGTTGGCGCAGCATTCATGTACACCGGTTTGATCATGGGTATCCTGATTATCCTGATCGCTTTCGTTATCCGTTTCCCTGGCCAGCAAGGCGCCAAAAAACAAATCGTTGTTACCGACAAGGATTTCAATTCTGGCGAAATGCTGAGAACACCACAATTCTGGGTTCTGTGGACCGCATTCTTTTCCGTTAACTTTGGTGGTTTGCTGCTGGTTGCCAACAGCGTCCCTTACGGTCGCAGCCTCGGTCTTGCCGCAGGTGTGCTGACGATCGGTGTTTCGATCCAGAACCTGTTCAATGGTGGTTGCCGTCCTTTCTGGGGTTTCGTTTCCGATAAAATCGGCCGTTACAAAACCATGTCCGTCGTTTTCGGTATCAATGCTGTTGTTCTCGCACTTTTCCCGACGATTGCTGCCTTGGGCGATGTAGCCTTTATCGCCATGTTGGCAATCGCATTCTTCACATGGGGTGGTAGCTACGCTCTGTTCCCATCGACCAACAGCGATATTTTCGGTACGGCATACTCTGCCAGAAACTATGGTTTCTTCTGGGCTGCAAAAGCAACTGCCTCGATCTTCGGTGGTGGTCTGGGTGCTGCAATTGCAACCAACTTCGGATGGAATACCGCTTTCCTGATTACTGCGATTACTTCTTTCATCGCATTTGCTCTGGCTACCTTCGTTATTCCAAGAATGGGCCGTCCAGTCAAGAAAATGGTCAAATTGTCTCCAGAAGAAAAAGCTGTACAT
SEQ ID NO:147(核糖体结合位点RBS4和Of_oxlT开放读码框)：
GCATCACCTAAAAATTATAAATAAATTAATAAACCACAATACTAAAAATTTAATAAAATGAATAATCCACAAACAGGACAATCAACAGGCCTCTTGGGCAATCGTTGGTTCTACTTGGTATTAGCAGTTTTGCTGATGTGTATGATCTCGGGTGTCCAATATTCCTGGACACTGTACGCTAACCCGGTTAAAGACAACCTTGGCGTTTCTTTGGCTGCGGTTCAGACGGCTTTCACACTCTCTCAGGTCATTCAAGCTGGTTCTCAGCCTGGTGGTGGTTACTTCGTTGATAAATTCGGTCCAAGAATTCCATTGATGTTCGGTGGTGCGATGGTTCTCGCTGGCTGGACCTTCATGGGTATGGTTGACAGTGTTCCTGCTCTGTATGCTCTTTATACTCTGGCCGGTGCAGGTGTTGGTATCGTTTACGGTATCGCGATGAACACGGCTAACAGATGGTTCCCGGACAAACGCGGTCTGGCTTCCGGTTTCACCGCTGCCGGTTACGGTCTGGGTGTTCTGCCGTTCCTGCCACTGATCAGCTCCGTTCTGAAAGTTGAAGGTGTTGGCGCAGCATTCATGTACACCGGTTTGATCATGGGTATCCTGATTATCCTGATCGCTTTCGTTATCCGTTTCCCTGGCCAGCAAGGCGCCAAAAAACAAATCGTTGTTACCGACAAGGATTTCAATTCTGGCGAAATGCTGAGAACACCACAATTCTGGGTTCTGTGGACCGCATTCTTTTCCGTTAACTTTGGTGGTTTGCTGCTGGTTGCCAACAGCGTCCCTTACGGTCGCAGCCTCGGTCTTGCCGCAGGTGTGCTGACGATCGGTGTTTCGATCCAGAACCTGTTCAATGGTGGTTGCCGTCCTTTCTGGGGTTTCGTTTCCGATAAAATCGGCCGTTACAAAACCATGTCCGTCGTTTTCGGTATCAATGCTGTTGTTCTCGCACTTTTCCCGACGATTGCTGCCTTGGGCGATGTAGCCTTTATCGCCATGTTGGCAATCGCATTCTTCACATGGGGTGGTAGCTACGCTCTGTTCCCATCGACCAACAGCGATATTTTCGGTACGGCATACTCTGCCAGAAACTATGGTTTCTTCTGGGCTGCAAAAGCAACTGCCTCGATCTTCGGTGGTGGTCTGGGTGCTGCAATTGCAACCAACTTCGGATGGAATACCGCTTTCCTGATTACTGCGATTACTTCTTTCATCGCATTTGCTCTGGCTACCTTCGTTATTCCAAGAATGGGCCGTCCAGTCAAGAAAATGGTCAAATTGTCTCCAGAAGAAAAAGCTGTACAT
SEQ ID NO:148(核糖体结合位点RBS5和Of_oxlT开放读码框)：
GCATCAACTACCTACATTAATAACCAACCCAAATGCCTAATCAATAAATATTATAAAATGAATAATCCACAAACAGGACAATCAACAGGCCTCTTGGGCAATCGTTGGTTCTACTTGGTATTAGCAGTTTTGCTGATGTGTATGATCTCGGGTGTCCAATATTCCTGGACACTGTACGCTAACCCGGTTAAAGACAACCTTGGCGTTTCTTTGGCTGCGGTTCAGACGGCTTTCACACTCTCTCAGGTCATTCAAGCTGGTTCTCAGCCTGGTGGTGGTTACTTCGTTGATAAATTCGGTCCAAGAATTCCATTGATGTTCGGTGGTGCGATGGTTCTCGCTGGCTGGACCTTCATGGGTATGGTTGACAGTGTTCCTGCTCTGTATGCTCTTTATACTCTGGCCGGTGCAGGTGTTGGTATCGTTTACGGTATCGCGATGAACACGGCTAACAGATGGTTCCCGGACAAACGCGGTCTGGCTTCCGGTTTCACCGCTGCCGGTTACGGTCTGGGTGTTCTGCCGTTCCTGCCACTGATCAGCTCCGTTCTGAAAGTTGAAGGTGTTGGCGCAGCATTCATGTACACCGGTTTGATCATGGGTATCCTGATTATCCTGATCGCTTTCGTTATCCGTTTCCCTGGCCAGCAAGGCGCCAAAAAACAAATCGTTGTTACCGACAAGGATTTCAATTCTGGCGAAATGCTGAGAACACCACAATTCTGGGTTCTGTGGACCGCATTCTTTTCCGTTAACTTTGGTGGTTTGCTGCTGGTTGCCAACAGCGTCCCTTACGGTCGCAGCCTCGGTCTTGCCGCAGGTGTGCTGACGATCGGTGTTTCGATCCAGAACCTGTTCAATGGTGGTTGCCGTCCTTTCTGGGGTTTCGTTTCCGATAAAATCGGCCGTTACAAAACCATGTCCGTCGTTTTCGGTATCAATGCTGTTGTTCTCGCACTTTTCCCGACGATTGCTGCCTTGGGCGATGTAGCCTTTATCGCCATGTTGGCAATCGCATTCTTCACATGGGGTGGTAGCTACGCTCTGTTCCCATCGACCAACAGCGATATTTTCGGTACGGCATACTCTGCCAGAAACTATGGTTTCTTCTGGGCTGCAAAAGCAACTGCCTCGATCTTCGGTGGTGGTCTGGGTGCTGCAATTGCAACCAACTTCGGATGGAATACCGCTTTCCTGATTACTGCGATTACTTCTTTCATCGCATTTGCTCTGGCTACCTTCGTTATTCCAAGAATGGGCCGTCCAGTCAAGAAAATGGTCAAATTGTCTCCAGAAGAAAAAGCTGTACAT
SEQ ID NO:149(核糖体结合位点RBS6和Of_oxlT开放读码框)：
GCATCATCTACCTATATTATTCATCAAACCATTTGATCCAACATTAAATATTAAAAAATGAATAATCCACAAACAGGACAATCAACAGGCCTCTTGGGCAATCGTTGGTTCTACTTGGTATTAGCAGTTTTGCTGATGTGTATGATCTCGGGTGTCCAATATTCCTGGACACTGTACGCTAACCCGGTTAAAGACAACCTTGGCGTTTCTTTGGCTGCGGTTCAGACGGCTTTCACACTCTCTCAGGTCATTCAAGCTGGTTCTCAGCCTGGTGGTGGTTACTTCGTTGATAAATTCGGTCCAAGAATTCCATTGATGTTCGGTGGTGCGATGGTTCTCGCTGGCTGGACCTTCATGGGTATGGTTGACAGTGTTCCTGCTCTGTATGCTCTTTATACTCTGGCCGGTGCAGGTGTTGGTATCGTTTACGGTATCGCGATGAACACGGCTAACAGATGGTTCCCGGACAAACGCGGTCTGGCTTCCGGTTTCACCGCTGCCGGTTACGGTCTGGGTGTTCTGCCGTTCCTGCCACTGATCAGCTCCGTTCTGAAAGTTGAAGGTGTTGGCGCAGCATTCATGTACACCGGTTTGATCATGGGTATCCTGATTATCCTGATCGCTTTCGTTATCCGTTTCCCTGGCCAGCAAGGCGCCAAAAAACAAATCGTTGTTACCGACAAGGATTTCAATTCTGGCGAAATGCTGAGAACACCACAATTCTGGGTTCTGTGGACCGCATTCTTTTCCGTTAACTTTGGTGGTTTGCTGCTGGTTGCCAACAGCGTCCCTTACGGTCGCAGCCTCGGTCTTGCCGCAGGTGTGCTGACGATCGGTGTTTCGATCCAGAACCTGTTCAATGGTGGTTGCCGTCCTTTCTGGGGTTTCGTTTCCGATAAAATCGGCCGTTACAAAACCATGTCCGTCGTTTTCGGTATCAATGCTGTTGTTCTCGCACTTTTCCCGACGATTGCTGCCTTGGGCGATGTAGCCTTTATCGCCATGTTGGCAATCGCATTCTTCACATGGGGTGGTAGCTACGCTCTGTTCCCATCGACCAACAGCGATATTTTCGGTACGGCATACTCTGCCAGAAACTATGGTTTCTTCTGGGCTGCAAAAGCAACTGCCTCGATCTTCGGTGGTGGTCTGGGTGCTGCAATTGCAACCAACTTCGGATGGAATACCGCTTTCCTGATTACTGCGATTACTTCTTTCATCGCATTTGCTCTGGCTACCTTCGTTATTCCAAGAATGGGCCGTCCAGTCAAGAAAATGGTCAAATTGTCTCCAGAAGAAAAAGCTGTACAT
SEQ ID NO:150(核糖体结合位点RBS7和Of_oxlT开放读码框)：
GCATCACCTACCTCAATTATTAATCATACCAACTGGATTTGATTTAAAAATAAGAAAATGAATAATCCACAAACAGGACAATCAACAGGCCTCTTGGGCAATCGTTGGTTCTACTTGGTATTAGCAGTTTTGCTGATGTGTATGATCTCGGGTGTCCAATATTCCTGGACACTGTACGCTAACCCGGTTAAAGACAACCTTGGCGTTTCTTTGGCTGCGGTTCAGACGGCTTTCACACTCTCTCAGGTCATTCAAGCTGGTTCTCAGCCTGGTGGTGGTTACTTCGTTGATAAATTCGGTCCAAGAATTCCATTGATGTTCGGTGGTGCGATGGTTCTCGCTGGCTGGACCTTCATGGGTATGGTTGACAGTGTTCCTGCTCTGTATGCTCTTTATACTCTGGCCGGTGCAGGTGTTGGTATCGTTTACGGTATCGCGATGAACACGGCTAACAGATGGTTCCCGGACAAACGCGGTCTGGCTTCCGGTTTCACCGCTGCCGGTTACGGTCTGGGTGTTCTGCCGTTCCTGCCACTGATCAGCTCCGTTCTGAAAGTTGAAGGTGTTGGCGCAGCATTCATGTACACCGGTTTGATCATGGGTATCCTGATTATCCTGATCGCTTTCGTTATCCGTTTCCCTGGCCAGCAAGGCGCCAAAAAACAAATCGTTGTTACCGACAAGGATTTCAATTCTGGCGAAATGCTGAGAACACCACAATTCTGGGTTCTGTGGACCGCATTCTTTTCCGTTAACTTTGGTGGTTTGCTGCTGGTTGCCAACAGCGTCCCTTACGGTCGCAGCCTCGGTCTTGCCGCAGGTGTGCTGACGATCGGTGTTTCGATCCAGAACCTGTTCAATGGTGGTTGCCGTCCTTTCTGGGGTTTCGTTTCCGATAAAATCGGCCGTTACAAAACCATGTCCGTCGTTTTCGGTATCAATGCTGTTGTTCTCGCACTTTTCCCGACGATTGCTGCCTTGGGCGATGTAGCCTTTATCGCCATGTTGGCAATCGCATTCTTCACATGGGGTGGTAGCTACGCTCTGTTCCCATCGACCAACAGCGATATTTTCGGTACGGCATACTCTGCCAGAAACTATGGTTTCTTCTGGGCTGCAAAAGCAACTGCCTCGATCTTCGGTGGTGGTCTGGGTGCTGCAATTGCAACCAACTTCGGATGGAATACCGCTTTCCTGATTACTGCGATTACTTCTTTCATCGCATTTGCTCTGGCTACCTTCGTTATTCCAAGAATGGGCCGTCCAGTCAAGAAAATGGTCAAATTGTCTCCAGAAGAAAAAGCTGTACAT
SEQ ID NO:151(核糖体结合位点RBS8和Of_oxlT开放读码框)：
GCATCACCTACCTTTATTACTCATCATACCAAATGGTAGCGGATTAAATATTAGAAAATGAATAATCCACAAACAGGACAATCAACAGGCCTCTTGGGCAATCGTTGGTTCTACTTGGTATTAGCAGTTTTGCTGATGTGTATGATCTCGGGTGTCCAATATTCCTGGACACTGTACGCTAACCCGGTTAAAGACAACCTTGGCGTTTCTTTGGCTGCGGTTCAGACGGCTTTCACACTCTCTCAGGTCATTCAAGCTGGTTCTCAGCCTGGTGGTGGTTACTTCGTTGATAAATTCGGTCCAAGAATTCCATTGATGTTCGGTGGTGCGATGGTTCTCGCTGGCTGGACCTTCATGGGTATGGTTGACAGTGTTCCTGCTCTGTATGCTCTTTATACTCTGGCCGGTGCAGGTGTTGGTATCGTTTACGGTATCGCGATGAACACGGCTAACAGATGGTTCCCGGACAAACGCGGTCTGGCTTCCGGTTTCACCGCTGCCGGTTACGGTCTGGGTGTTCTGCCGTTCCTGCCACTGATCAGCTCCGTTCTGAAAGTTGAAGGTGTTGGCGCAGCATTCATGTACACCGGTTTGATCATGGGTATCCTGATTATCCTGATCGCTTTCGTTATCCGTTTCCCTGGCCAGCAAGGCGCCAAAAAACAAATCGTTGTTACCGACAAGGATTTCAATTCTGGCGAAATGCTGAGAACACCACAATTCTGGGTTCTGTGGACCGCATTCTTTTCCGTTAACTTTGGTGGTTTGCTGCTGGTTGCCAACAGCGTCCCTTACGGTCGCAGCCTCGGTCTTGCCGCAGGTGTGCTGACGATCGGTGTTTCGATCCAGAACCTGTTCAATGGTGGTTGCCGTCCTTTCTGGGGTTTCGTTTCCGATAAAATCGGCCGTTACAAAACCATGTCCGTCGTTTTCGGTATCAATGCTGTTGTTCTCGCACTTTTCCCGACGATTGCTGCCTTGGGCGATGTAGCCTTTATCGCCATGTTGGCAATCGCATTCTTCACATGGGGTGGTAGCTACGCTCTGTTCCCATCGACCAACAGCGATATTTTCGGTACGGCATACTCTGCCAGAAACTATGGTTTCTTCTGGGCTGCAAAAGCAACTGCCTCGATCTTCGGTGGTGGTCTGGGTGCTGCAATTGCAACCAACTTCGGATGGAATACCGCTTTCCTGATTACTGCGATTACTTCTTTCATCGCATTTGCTCTGGCTACCTTCGTTATTCCAAGAATGGGCCGTCCAGTCAAGAAAATGGTCAAATTGTCTCCAGAAGAAAAAGCTGTACAT
SEQ ID NO:152(核糖体结合位点'RBS1和Sc_YEL020C(密码子优化的)开放读码框)：
GCATCATCTACCTTAATTAATCAACAAACCAATTACCAACCTAAAAAATATAAGAAAATGACTACCACAGCAACGCAGCATTTTGCCCAGTTACTCCAGAAGTACGGAATTGATACTGTCTTCGGCATAGTAGGTATTCCGATCGTGCAGTTGGCCGACACTATGGTCGCGAATGGAATTAAGTTTATACCGTGTCGCAATGAGCAAGCCGCAAGTTACGCGGCAAGCGCCTACGGCTACATCTCGGACAAACCTGGTGTCCTGTTAATCGTGGGAGGGCCTGGCCTTATTCATGCCTTAGCGGGGATCTATAATAGTATGTCGAACCGCTGGCCCTTGTTGGTCATCGCCGGATCCAGCAGCCAGTCAGACATCCACAAGGGAGGTTTCCAGGAACTTGACCAGGTGTCCTTGCTAAGTCCGTTCCTAAAATTCACTGGTAAACTGACGCCGGATAACATCGATATGATAACCCAAAAAGCACTCAACTATTGCATTCAGGGAACCGCCGGAGTGAGCTATATCGATGTCCCCGCTGACTTCATCGAATACGAGAAGCCGCTTGAGGGCAATGATAGAACTGGTAACGAGCTGCCAATGATCCTGACGCCCAATATCTGCGGTCCAGACCCCAGTAAAATTAAGAAGGTGGTGCAATTGATACTCCAGCACAAGAACAAGAACATATTAATAGTGATAGGTAAAGGAGCAGTGAAGAATAGCCATGAGATTAGACGCTTGGTTAACACCTTTAACTTGCCGTTCCTTCCGACTCCCATGGCGAAAGGCATAGTGCCCGATAGCAGCCCTTTAAATGTGAGCAGCGCCCGGAGCCAGGCTCTTAAGATTGCTGACATCGTGTTAGTATTGGGAGCTCGCCTGAATTGGATCCTTCACTTCGGAACGAGTCCAAAGTGGAATAGCGAGAGTATTTTTATACAATTTGACAGTAACCCGGAAACGCTCGGAGACAATAACGTTAGTCCCGGAGCAGATTTAAGTATCTGGGGGGACATCGGACTATCGGTTACTGCACTGGTAGAAGAGCTCACGAGACAGGACAGTTGTTGGAAATACTCGGGAGTCAAACAGGAGATCAGGGAAAAGATACAGTTAAACCAGACCAGATTGCTCCGTAAGGAAAAAACGCGTGGAGCTCAGCTTAACTATAATCAAGTGTACGGCACCCTTCGTCCGTTAATTGACGACTATCGCACGATCTTGGTCACAGAAGGTGCCAACACAATGGACATCGCTAGAATAAGTTTTCCGACTGATGCACCTCGCAGAAGACTTGATGCTGGTACAAATGCTACAATGGGTATAGGTTTAGGTTACGCATTAGCGTGTAAAGCCAGCCACCCTGAGTTAGACGTTGTTTTAATCCAAGGCGACAGTGCCTTCGGTTTTAGCGCTATGGAGATCGAGACTGCCGTCCGTTGCCAATTAGCCCTTGTGATTGTCGTAATGAATAACTCTGGGATATATCACGGTGAGAAAGACATCGAAGGAGATCTGCCCCCGACCGCTTTGTCAAAAAATTGTAGATATGACTTGGTCGGTAAGGGTTTAGGAGCTAACGACTTCTTCGTCAATACCATCTCAGAGTTATCTCGCTGTTTTCAGCAGGCAGTTCAATTGAGCAGAACCAAGCGCGAAACCTCCGTCATTAATGTCATAATCGAACCGGGAGAGCAGAAACAGATCGCGTTCGCATGGCAAAACAAGCCTAGACTG
SEQ ID NO:153(核糖体结合位点RBS2和Sc_YEL020C(密码子优化的)开放读码框)：
GCATCAACTACCTTTATTATTCAACAAACCAATTTAACATATTAAAAAAATAAAAAAATGACTACCACAGCAACGCAGCATTTTGCCCAGTTACTCCAGAAGTACGGAATTGATACTGTCTTCGGCATAGTAGGTATTCCGATCGTGCAGTTGGCCGACACTATGGTCGCGAATGGAATTAAGTTTATACCGTGTCGCAATGAGCAAGCCGCAAGTTACGCGGCAAGCGCCTACGGCTACATCTCGGACAAACCTGGTGTCCTGTTAATCGTGGGAGGGCCTGGCCTTATTCATGCCTTAGCGGGGATCTATAATAGTATGTCGAACCGCTGGCCCTTGTTGGTCATCGCCGGATCCAGCAGCCAGTCAGACATCCACAAGGGAGGTTTCCAGGAACTTGACCAGGTGTCCTTGCTAAGTCCGTTCCTAAAATTCACTGGTAAACTGACGCCGGATAACATCGATATGATAACCCAAAAAGCACTCAACTATTGCATTCAGGGAACCGCCGGAGTGAGCTATATCGATGTCCCCGCTGACTTCATCGAATACGAGAAGCCGCTTGAGGGCAATGATAGAACTGGTAACGAGCTGCCAATGATCCTGACGCCCAATATCTGCGGTCCAGACCCCAGTAAAATTAAGAAGGTGGTGCAATTGATACTCCAGCACAAGAACAAGAACATATTAATAGTGATAGGTAAAGGAGCAGTGAAGAATAGCCATGAGATTAGACGCTTGGTTAACACCTTTAACTTGCCGTTCCTTCCGACTCCCATGGCGAAAGGCATAGTGCCCGATAGCAGCCCTTTAAATGTGAGCAGCGCCCGGAGCCAGGCTCTTAAGATTGCTGACATCGTGTTAGTATTGGGAGCTCGCCTGAATTGGATCCTTCACTTCGGAACGAGTCCAAAGTGGAATAGCGAGAGTATTTTTATACAATTTGACAGTAACCCGGAAACGCTCGGAGACAATAACGTTAGTCCCGGAGCAGATTTAAGTATCTGGGGGGACATCGGACTATCGGTTACTGCACTGGTAGAAGAGCTCACGAGACAGGACAGTTGTTGGAAATACTCGGGAGTCAAACAGGAGATCAGGGAAAAGATACAGTTAAACCAGACCAGATTGCTCCGTAAGGAAAAAACGCGTGGAGCTCAGCTTAACTATAATCAAGTGTACGGCACCCTTCGTCCGTTAATTGACGACTATCGCACGATCTTGGTCACAGAAGGTGCCAACACAATGGACATCGCTAGAATAAGTTTTCCGACTGATGCACCTCGCAGAAGACTTGATGCTGGTACAAATGCTACAATGGGTATAGGTTTAGGTTACGCATTAGCGTGTAAAGCCAGCCACCCTGAGTTAGACGTTGTTTTAATCCAAGGCGACAGTGCCTTCGGTTTTAGCGCTATGGAGATCGAGACTGCCGTCCGTTGCCAATTAGCCCTTGTGATTGTCGTAATGAATAACTCTGGGATATATCACGGTGAGAAAGACATCGAAGGAGATCTGCCCCCGACCGCTTTGTCAAAAAATTGTAGATATGACTTGGTCGGTAAGGGTTTAGGAGCTAACGACTTCTTCGTCAATACCATCTCAGAGTTATCTCGCTGTTTTCAGCAGGCAGTTCAATTGAGCAGAACCAAGCGCGAAACCTCCGTCATTAATGTCATAATCGAACCGGGAGAGCAGAAACAGATCGCGTTCGCATGGCAAAACAAGCCTAGACTG
SEQ ID NO:154(核糖体结合位点RBS3和Sc_YEL020C(密码子优化的)开放读码框)：
GCATCTACCCCTAACAACCCATAAATTTAACTTTAAAAAATTTTTAAATATAATAAAATGACTACCACAGCAACGCAGCATTTTGCCCAGTTACTCCAGAAGTACGGAATTGATACTGTCTTCGGCATAGTAGGTATTCCGATCGTGCAGTTGGCCGACACTATGGTCGCGAATGGAATTAAGTTTATACCGTGTCGCAATGAGCAAGCCGCAAGTTACGCGGCAAGCGCCTACGGCTACATCTCGGACAAACCTGGTGTCCTGTTAATCGTGGGAGGGCCTGGCCTTATTCATGCCTTAGCGGGGATCTATAATAGTATGTCGAACCGCTGGCCCTTGTTGGTCATCGCCGGATCCAGCAGCCAGTCAGACATCCACAAGGGAGGTTTCCAGGAACTTGACCAGGTGTCCTTGCTAAGTCCGTTCCTAAAATTCACTGGTAAACTGACGCCGGATAACATCGATATGATAACCCAAAAAGCACTCAACTATTGCATTCAGGGAACCGCCGGAGTGAGCTATATCGATGTCCCCGCTGACTTCATCGAATACGAGAAGCCGCTTGAGGGCAATGATAGAACTGGTAACGAGCTGCCAATGATCCTGACGCCCAATATCTGCGGTCCAGACCCCAGTAAAATTAAGAAGGTGGTGCAATTGATACTCCAGCACAAGAACAAGAACATATTAATAGTGATAGGTAAAGGAGCAGTGAAGAATAGCCATGAGATTAGACGCTTGGTTAACACCTTTAACTTGCCGTTCCTTCCGACTCCCATGGCGAAAGGCATAGTGCCCGATAGCAGCCCTTTAAATGTGAGCAGCGCCCGGAGCCAGGCTCTTAAGATTGCTGACATCGTGTTAGTATTGGGAGCTCGCCTGAATTGGATCCTTCACTTCGGAACGAGTCCAAAGTGGAATAGCGAGAGTATTTTTATACAATTTGACAGTAACCCGGAAACGCTCGGAGACAATAACGTTAGTCCCGGAGCAGATTTAAGTATCTGGGGGGACATCGGACTATCGGTTACTGCACTGGTAGAAGAGCTCACGAGACAGGACAGTTGTTGGAAATACTCGGGAGTCAAACAGGAGATCAGGGAAAAGATACAGTTAAACCAGACCAGATTGCTCCGTAAGGAAAAAACGCGTGGAGCTCAGCTTAACTATAATCAAGTGTACGGCACCCTTCGTCCGTTAATTGACGACTATCGCACGATCTTGGTCACAGAAGGTGCCAACACAATGGACATCGCTAGAATAAGTTTTCCGACTGATGCACCTCGCAGAAGACTTGATGCTGGTACAAATGCTACAATGGGTATAGGTTTAGGTTACGCATTAGCGTGTAAAGCCAGCCACCCTGAGTTAGACGTTGTTTTAATCCAAGGCGACAGTGCCTTCGGTTTTAGCGCTATGGAGATCGAGACTGCCGTCCGTTGCCAATTAGCCCTTGTGATTGTCGTAATGAATAACTCTGGGATATATCACGGTGAGAAAGACATCGAAGGAGATCTGCCCCCGACCGCTTTGTCAAAAAATTGTAGATATGACTTGGTCGGTAAGGGTTTAGGAGCTAACGACTTCTTCGTCAATACCATCTCAGAGTTATCTCGCTGTTTTCAGCAGGCAGTTCAATTGAGCAGAACCAAGCGCGAAACCTCCGTCATTAATGTCATAATCGAACCGGGAGAGCAGAAACAGATCGCGTTCGCATGGCAAAACAAGCCTAGACTG
SEQ ID NO:155(核糖体结合位点RBS4和Sc_YEL020C(密码子优化的)开放读码框)：
GCATCATCTACCTTCATTATTAAACAAACCAAATTAATATCATAAAAAAATAAAAAAATGACTACCACAGCAACGCAGCATTTTGCCCAGTTACTCCAGAAGTACGGAATTGATACTGTCTTCGGCATAGTAGGTATTCCGATCGTGCAGTTGGCCGACACTATGGTCGCGAATGGAATTAAGTTTATACCGTGTCGCAATGAGCAAGCCGCAAGTTACGCGGCAAGCGCCTACGGCTACATCTCGGACAAACCTGGTGTCCTGTTAATCGTGGGAGGGCCTGGCCTTATTCATGCCTTAGCGGGGATCTATAATAGTATGTCGAACCGCTGGCCCTTGTTGGTCATCGCCGGATCCAGCAGCCAGTCAGACATCCACAAGGGAGGTTTCCAGGAACTTGACCAGGTGTCCTTGCTAAGTCCGTTCCTAAAATTCACTGGTAAACTGACGCCGGATAACATCGATATGATAACCCAAAAAGCACTCAACTATTGCATTCAGGGAACCGCCGGAGTGAGCTATATCGATGTCCCCGCTGACTTCATCGAATACGAGAAGCCGCTTGAGGGCAATGATAGAACTGGTAACGAGCTGCCAATGATCCTGACGCCCAATATCTGCGGTCCAGACCCCAGTAAAATTAAGAAGGTGGTGCAATTGATACTCCAGCACAAGAACAAGAACATATTAATAGTGATAGGTAAAGGAGCAGTGAAGAATAGCCATGAGATTAGACGCTTGGTTAACACCTTTAACTTGCCGTTCCTTCCGACTCCCATGGCGAAAGGCATAGTGCCCGATAGCAGCCCTTTAAATGTGAGCAGCGCCCGGAGCCAGGCTCTTAAGATTGCTGACATCGTGTTAGTATTGGGAGCTCGCCTGAATTGGATCCTTCACTTCGGAACGAGTCCAAAGTGGAATAGCGAGAGTATTTTTATACAATTTGACAGTAACCCGGAAACGCTCGGAGACAATAACGTTAGTCCCGGAGCAGATTTAAGTATCTGGGGGGACATCGGACTATCGGTTACTGCACTGGTAGAAGAGCTCACGAGACAGGACAGTTGTTGGAAATACTCGGGAGTCAAACAGGAGATCAGGGAAAAGATACAGTTAAACCAGACCAGATTGCTCCGTAAGGAAAAAACGCGTGGAGCTCAGCTTAACTATAATCAAGTGTACGGCACCCTTCGTCCGTTAATTGACGACTATCGCACGATCTTGGTCACAGAAGGTGCCAACACAATGGACATCGCTAGAATAAGTTTTCCGACTGATGCACCTCGCAGAAGACTTGATGCTGGTACAAATGCTACAATGGGTATAGGTTTAGGTTACGCATTAGCGTGTAAAGCCAGCCACCCTGAGTTAGACGTTGTTTTAATCCAAGGCGACAGTGCCTTCGGTTTTAGCGCTATGGAGATCGAGACTGCCGTCCGTTGCCAATTAGCCCTTGTGATTGTCGTAATGAATAACTCTGGGATATATCACGGTGAGAAAGACATCGAAGGAGATCTGCCCCCGACCGCTTTGTCAAAAAATTGTAGATATGACTTGGTCGGTAAGGGTTTAGGAGCTAACGACTTCTTCGTCAATACCATCTCAGAGTTATCTCGCTGTTTTCAGCAGGCAGTTCAATTGAGCAGAACCAAGCGCGAAACCTCCGTCATTAATGTCATAATCGAACCGGGAGAGCAGAAACAGATCGCGTTCGCATGGCAAAACAAGCCTAGACTG
SEQ ID NO:156(核糖体结合位点RBS5和Sc_YEL020C(密码子优化的)开放读码框)：
GCATCTACCCCTAACAACCCATAAATTTAACTTTTCTACAAAAAAAAATATAAAAAAATGACTACCACAGCAACGCAGCATTTTGCCCAGTTACTCCAGAAGTACGGAATTGATACTGTCTTCGGCATAGTAGGTATTCCGATCGTGCAGTTGGCCGACACTATGGTCGCGAATGGAATTAAGTTTATACCGTGTCGCAATGAGCAAGCCGCAAGTTACGCGGCAAGCGCCTACGGCTACATCTCGGACAAACCTGGTGTCCTGTTAATCGTGGGAGGGCCTGGCCTTATTCATGCCTTAGCGGGGATCTATAATAGTATGTCGAACCGCTGGCCCTTGTTGGTCATCGCCGGATCCAGCAGCCAGTCAGACATCCACAAGGGAGGTTTCCAGGAACTTGACCAGGTGTCCTTGCTAAGTCCGTTCCTAAAATTCACTGGTAAACTGACGCCGGATAACATCGATATGATAACCCAAAAAGCACTCAACTATTGCATTCAGGGAACCGCCGGAGTGAGCTATATCGATGTCCCCGCTGACTTCATCGAATACGAGAAGCCGCTTGAGGGCAATGATAGAACTGGTAACGAGCTGCCAATGATCCTGACGCCCAATATCTGCGGTCCAGACCCCAGTAAAATTAAGAAGGTGGTGCAATTGATACTCCAGCACAAGAACAAGAACATATTAATAGTGATAGGTAAAGGAGCAGTGAAGAATAGCCATGAGATTAGACGCTTGGTTAACACCTTTAACTTGCCGTTCCTTCCGACTCCCATGGCGAAAGGCATAGTGCCCGATAGCAGCCCTTTAAATGTGAGCAGCGCCCGGAGCCAGGCTCTTAAGATTGCTGACATCGTGTTAGTATTGGGAGCTCGCCTGAATTGGATCCTTCACTTCGGAACGAGTCCAAAGTGGAATAGCGAGAGTATTTTTATACAATTTGACAGTAACCCGGAAACGCTCGGAGACAATAACGTTAGTCCCGGAGCAGATTTAAGTATCTGGGGGGACATCGGACTATCGGTTACTGCACTGGTAGAAGAGCTCACGAGACAGGACAGTTGTTGGAAATACTCGGGAGTCAAACAGGAGATCAGGGAAAAGATACAGTTAAACCAGACCAGATTGCTCCGTAAGGAAAAAACGCGTGGAGCTCAGCTTAACTATAATCAAGTGTACGGCACCCTTCGTCCGTTAATTGACGACTATCGCACGATCTTGGTCACAGAAGGTGCCAACACAATGGACATCGCTAGAATAAGTTTTCCGACTGATGCACCTCGCAGAAGACTTGATGCTGGTACAAATGCTACAATGGGTATAGGTTTAGGTTACGCATTAGCGTGTAAAGCCAGCCACCCTGAGTTAGACGTTGTTTTAATCCAAGGCGACAGTGCCTTCGGTTTTAGCGCTATGGAGATCGAGACTGCCGTCCGTTGCCAATTAGCCCTTGTGATTGTCGTAATGAATAACTCTGGGATATATCACGGTGAGAAAGACATCGAAGGAGATCTGCCCCCGACCGCTTTGTCAAAAAATTGTAGATATGACTTGGTCGGTAAGGGTTTAGGAGCTAACGACTTCTTCGTCAATACCATCTCAGAGTTATCTCGCTGTTTTCAGCAGGCAGTTCAATTGAGCAGAACCAAGCGCGAAACCTCCGTCATTAATGTCATAATCGAACCGGGAGAGCAGAAACAGATCGCGTTCGCATGGCAAAACAAGCCTAGACTG
SEQ ID NO:157(核糖体结合位点RBS6和Sc_YEL020C(密码子优化的)开放读码框)：
GCATCACCTACCTACATTAATAACCATACCATCTCAGTTTTAAATAAATTTTAAAAAATGACTACCACAGCAACGCAGCATTTTGCCCAGTTACTCCAGAAGTACGGAATTGATACTGTCTTCGGCATAGTAGGTATTCCGATCGTGCAGTTGGCCGACACTATGGTCGCGAATGGAATTAAGTTTATACCGTGTCGCAATGAGCAAGCCGCAAGTTACGCGGCAAGCGCCTACGGCTACATCTCGGACAAACCTGGTGTCCTGTTAATCGTGGGAGGGCCTGGCCTTATTCATGCCTTAGCGGGGATCTATAATAGTATGTCGAACCGCTGGCCCTTGTTGGTCATCGCCGGATCCAGCAGCCAGTCAGACATCCACAAGGGAGGTTTCCAGGAACTTGACCAGGTGTCCTTGCTAAGTCCGTTCCTAAAATTCACTGGTAAACTGACGCCGGATAACATCGATATGATAACCCAAAAAGCACTCAACTATTGCATTCAGGGAACCGCCGGAGTGAGCTATATCGATGTCCCCGCTGACTTCATCGAATACGAGAAGCCGCTTGAGGGCAATGATAGAACTGGTAACGAGCTGCCAATGATCCTGACGCCCAATATCTGCGGTCCAGACCCCAGTAAAATTAAGAAGGTGGTGCAATTGATACTCCAGCACAAGAACAAGAACATATTAATAGTGATAGGTAAAGGAGCAGTGAAGAATAGCCATGAGATTAGACGCTTGGTTAACACCTTTAACTTGCCGTTCCTTCCGACTCCCATGGCGAAAGGCATAGTGCCCGATAGCAGCCCTTTAAATGTGAGCAGCGCCCGGAGCCAGGCTCTTAAGATTGCTGACATCGTGTTAGTATTGGGAGCTCGCCTGAATTGGATCCTTCACTTCGGAACGAGTCCAAAGTGGAATAGCGAGAGTATTTTTATACAATTTGACAGTAACCCGGAAACGCTCGGAGACAATAACGTTAGTCCCGGAGCAGATTTAAGTATCTGGGGGGACATCGGACTATCGGTTACTGCACTGGTAGAAGAGCTCACGAGACAGGACAGTTGTTGGAAATACTCGGGAGTCAAACAGGAGATCAGGGAAAAGATACAGTTAAACCAGACCAGATTGCTCCGTAAGGAAAAAACGCGTGGAGCTCAGCTTAACTATAATCAAGTGTACGGCACCCTTCGTCCGTTAATTGACGACTATCGCACGATCTTGGTCACAGAAGGTGCCAACACAATGGACATCGCTAGAATAAGTTTTCCGACTGATGCACCTCGCAGAAGACTTGATGCTGGTACAAATGCTACAATGGGTATAGGTTTAGGTTACGCATTAGCGTGTAAAGCCAGCCACCCTGAGTTAGACGTTGTTTTAATCCAAGGCGACAGTGCCTTCGGTTTTAGCGCTATGGAGATCGAGACTGCCGTCCGTTGCCAATTAGCCCTTGTGATTGTCGTAATGAATAACTCTGGGATATATCACGGTGAGAAAGACATCGAAGGAGATCTGCCCCCGACCGCTTTGTCAAAAAATTGTAGATATGACTTGGTCGGTAAGGGTTTAGGAGCTAACGACTTCTTCGTCAATACCATCTCAGAGTTATCTCGCTGTTTTCAGCAGGCAGTTCAATTGAGCAGAACCAAGCGCGAAACCTCCGTCATTAATGTCATAATCGAACCGGGAGAGCAGAAACAGATCGCGTTCGCATGGCAAAACAAGCCTAGACTG
SEQ ID NO:158(核糖体结合位点RBS7和Sc_YEL020C(密码子优化的)开放读码框)：
GCATCACCTACCTTAATTAATCACCATTCCATATCCTTTCAAAAAAAATATAAAAAAATGACTACCACAGCAACGCAGCATTTTGCCCAGTTACTCCAGAAGTACGGAATTGATACTGTCTTCGGCATAGTAGGTATTCCGATCGTGCAGTTGGCCGACACTATGGTCGCGAATGGAATTAAGTTTATACCGTGTCGCAATGAGCAAGCCGCAAGTTACGCGGCAAGCGCCTACGGCTACATCTCGGACAAACCTGGTGTCCTGTTAATCGTGGGAGGGCCTGGCCTTATTCATGCCTTAGCGGGGATCTATAATAGTATGTCGAACCGCTGGCCCTTGTTGGTCATCGCCGGATCCAGCAGCCAGTCAGACATCCACAAGGGAGGTTTCCAGGAACTTGACCAGGTGTCCTTGCTAAGTCCGTTCCTAAAATTCACTGGTAAACTGACGCCGGATAACATCGATATGATAACCCAAAAAGCACTCAACTATTGCATTCAGGGAACCGCCGGAGTGAGCTATATCGATGTCCCCGCTGACTTCATCGAATACGAGAAGCCGCTTGAGGGCAATGATAGAACTGGTAACGAGCTGCCAATGATCCTGACGCCCAATATCTGCGGTCCAGACCCCAGTAAAATTAAGAAGGTGGTGCAATTGATACTCCAGCACAAGAACAAGAACATATTAATAGTGATAGGTAAAGGAGCAGTGAAGAATAGCCATGAGATTAGACGCTTGGTTAACACCTTTAACTTGCCGTTCCTTCCGACTCCCATGGCGAAAGGCATAGTGCCCGATAGCAGCCCTTTAAATGTGAGCAGCGCCCGGAGCCAGGCTCTTAAGATTGCTGACATCGTGTTAGTATTGGGAGCTCGCCTGAATTGGATCCTTCACTTCGGAACGAGTCCAAAGTGGAATAGCGAGAGTATTTTTATACAATTTGACAGTAACCCGGAAACGCTCGGAGACAATAACGTTAGTCCCGGAGCAGATTTAAGTATCTGGGGGGACATCGGACTATCGGTTACTGCACTGGTAGAAGAGCTCACGAGACAGGACAGTTGTTGGAAATACTCGGGAGTCAAACAGGAGATCAGGGAAAAGATACAGTTAAACCAGACCAGATTGCTCCGTAAGGAAAAAACGCGTGGAGCTCAGCTTAACTATAATCAAGTGTACGGCACCCTTCGTCCGTTAATTGACGACTATCGCACGATCTTGGTCACAGAAGGTGCCAACACAATGGACATCGCTAGAATAAGTTTTCCGACTGATGCACCTCGCAGAAGACTTGATGCTGGTACAAATGCTACAATGGGTATAGGTTTAGGTTACGCATTAGCGTGTAAAGCCAGCCACCCTGAGTTAGACGTTGTTTTAATCCAAGGCGACAGTGCCTTCGGTTTTAGCGCTATGGAGATCGAGACTGCCGTCCGTTGCCAATTAGCCCTTGTGATTGTCGTAATGAATAACTCTGGGATATATCACGGTGAGAAAGACATCGAAGGAGATCTGCCCCCGACCGCTTTGTCAAAAAATTGTAGATATGACTTGGTCGGTAAGGGTTTAGGAGCTAACGACTTCTTCGTCAATACCATCTCAGAGTTATCTCGCTGTTTTCAGCAGGCAGTTCAATTGAGCAGAACCAAGCGCGAAACCTCCGTCATTAATGTCATAATCGAACCGGGAGAGCAGAAACAGATCGCGTTCGCATGGCAAAACAAGCCTAGACTG
SEQ ID NO:159(核糖体结合位点RBS8和Sc_YEL020C(密码子优化的)开放读码框)：
GCATCACCTACCTCTATTAATCATCATTCCAATTTAGGCTCATTAAATATTAGAAAATGACTACCACAGCAACGCAGCATTTTGCCCAGTTACTCCAGAAGTACGGAATTGATACTGTCTTCGGCATAGTAGGTATTCCGATCGTGCAGTTGGCCGACACTATGGTCGCGAATGGAATTAAGTTTATACCGTGTCGCAATGAGCAAGCCGCAAGTTACGCGGCAAGCGCCTACGGCTACATCTCGGACAAACCTGGTGTCCTGTTAATCGTGGGAGGGCCTGGCCTTATTCATGCCTTAGCGGGGATCTATAATAGTATGTCGAACCGCTGGCCCTTGTTGGTCATCGCCGGATCCAGCAGCCAGTCAGACATCCACAAGGGAGGTTTCCAGGAACTTGACCAGGTGTCCTTGCTAAGTCCGTTCCTAAAATTCACTGGTAAACTGACGCCGGATAACATCGATATGATAACCCAAAAAGCACTCAACTATTGCATTCAGGGAACCGCCGGAGTGAGCTATATCGATGTCCCCGCTGACTTCATCGAATACGAGAAGCCGCTTGAGGGCAATGATAGAACTGGTAACGAGCTGCCAATGATCCTGACGCCCAATATCTGCGGTCCAGACCCCAGTAAAATTAAGAAGGTGGTGCAATTGATACTCCAGCACAAGAACAAGAACATATTAATAGTGATAGGTAAAGGAGCAGTGAAGAATAGCCATGAGATTAGACGCTTGGTTAACACCTTTAACTTGCCGTTCCTTCCGACTCCCATGGCGAAAGGCATAGTGCCCGATAGCAGCCCTTTAAATGTGAGCAGCGCCCGGAGCCAGGCTCTTAAGATTGCTGACATCGTGTTAGTATTGGGAGCTCGCCTGAATTGGATCCTTCACTTCGGAACGAGTCCAAAGTGGAATAGCGAGAGTATTTTTATACAATTTGACAGTAACCCGGAAACGCTCGGAGACAATAACGTTAGTCCCGGAGCAGATTTAAGTATCTGGGGGGACATCGGACTATCGGTTACTGCACTGGTAGAAGAGCTCACGAGACAGGACAGTTGTTGGAAATACTCGGGAGTCAAACAGGAGATCAGGGAAAAGATACAGTTAAACCAGACCAGATTGCTCCGTAAGGAAAAAACGCGTGGAGCTCAGCTTAACTATAATCAAGTGTACGGCACCCTTCGTCCGTTAATTGACGACTATCGCACGATCTTGGTCACAGAAGGTGCCAACACAATGGACATCGCTAGAATAAGTTTTCCGACTGATGCACCTCGCAGAAGACTTGATGCTGGTACAAATGCTACAATGGGTATAGGTTTAGGTTACGCATTAGCGTGTAAAGCCAGCCACCCTGAGTTAGACGTTGTTTTAATCCAAGGCGACAGTGCCTTCGGTTTTAGCGCTATGGAGATCGAGACTGCCGTCCGTTGCCAATTAGCCCTTGTGATTGTCGTAATGAATAACTCTGGGATATATCACGGTGAGAAAGACATCGAAGGAGATCTGCCCCCGACCGCTTTGTCAAAAAATTGTAGATATGACTTGGTCGGTAAGGGTTTAGGAGCTAACGACTTCTTCGTCAATACCATCTCAGAGTTATCTCGCTGTTTTCAGCAGGCAGTTCAATTGAGCAGAACCAAGCGCGAAACCTCCGTCATTAATGTCATAATCGAACCGGGAGAGCAGAAACAGATCGCGTTCGCATGGCAAAACAAGCCTAGACTG
SEQ ID NO:160(核糖体结合位点RBS1b和Sc_YEL020C(密码子优化的)开放读码框)：
GCATCATCTACCTTAATTAATCAACAAACCAATTACCAACCTAAAAAATATAAGAAAATGACTACCACAGCAACGCAGCATTTTGCCCAGTTACTCCAGAAGTACGGAATTGATACTGTCTTCGGCATAGTAGGTATTCCGATCGTGCAGTTGGCCGACACTATGGTCGCGAATGGAATTAAGTTTATACCGTGTCGCAATGAGCAAGCCGCAAGTTACGCGGCAAGCGCCTACGGCTACATCTCGGACAAACCTGGTGTCCTGTTAATCGTGGGAGGGCCTGGCCTTATTCATGCCTTAGCGGGGATCTATAATAGTATGTCGAACCGCTGGCCCTTGTTGGTCATCGCCGGATCCAGCAGCCAGTCAGACATCCACAAGGGAGGTTTCCAGGAACTTGACCAGGTGTCCTTGCTAAGTCCGTTCCTAAAATTCACTGGTAAACTGACGCCGGATAACATCGATATGATAACCCAAAAAGCACTCAACTATTGCATTCAGGGAACCGCCGGAGTGAGCTATATCGATGTCCCCGCTGACTTCATCGAATACGAGAAGCCGCTTGAGGGCAATGATAGAACTGGTAACGAGCTGCCAATGATCCTGACGCCCAATATCTGCGGTCCAGACCCCAGTAAAATTAAGAAGGTGGTGCAATTGATACTCCAGCACAAGAACAAGAACATATTAATAGTGATAGGTAAAGGAGCAGTGAAGAATAGCCATGAGATTAGACGCTTGGTTAACACCTTTAACTTGCCGTTCCTTCCGACTCCCATGGCGAAAGGCATAGTGCCCGATAGCAGCCCTTTAAATGTGAGCAGCGCCCGGAGCCAGGCTCTTAAGATTGCTGACATCGTGTTAGTATTGGGAGCTCGCCTGAATTGGATCCTTCACTTCGGAACGAGTCCAAAGTGGAATAGCGAGAGTATTTTTATACAATTTGACAGTAACCCGGAAACGCTCGGAGACAATAACGTTAGTCCCGGAGCAGATTTAAGTATCTGGGGGGACATCGGACTATCGGTTACTGCACTGGTAGAAGAGCTCACGAGACAGGACAGTTGTTGGAAATACTCGGGAGTCAAACAGGAGATCAGGGAAAAGATACAGTTAAACCAGACCAGATTGCTCCGTAAGGAAAAAACGCGTGGAGCTCAGCTTAACTATAATCAAGTGTACGGCACCCTTCGTCCGTTAATTGACGACTATCGCACGATCTTGGTCACAGAAGGTGCCAACACAATGGACATCGCTAGAATAAGTTTTCCGACTGATGCACCTCGCAGAAGACTTGATGCTGGTACAAATGCTACAATGGGTATAGGTTTAGGTTACGCATTAGCGTGTAAAGCCAGCCACCCTGAGTTAGACGTTGTTTTAATCCAAGGCGACAGTGCCTTCGGTTTTAGCGCTATGGAGATCGAGACTGCCGTCCGTTGCCAATTAGCCCTTGTGATTGTCGTAATGAATAACTCTGGGATATATCACGGTGAGAAAGACATCGAAGGAGATCTGCCCCCGACCGCTTTGTCAAAAAATTGTAGATATGACTTGGTCGGTAAGGGTTTAGGAGCTAACGACTTCTTCGTCAATACCATCTCAGAGTTATCTCGCTGTTTTCAGCAGGCAGTTCAATTGAGCAGAACCAAGCGCGAAACCTCCGTCATTAATGTCATAATCGAACCGGGAGAGCAGAAACAGATCGCGTTCGCATGGCAAAACAAGCCTAGACTG
SEQ ID NO:161(核糖体结合位点RBS2b和Sc_YEL020C(密码子优化的)开放读码框)：
GCATCAACTACCTTTATTATTCAACAAACCAATTTAACATAGTAAAAAAATAAAAAAATGACTACCACAGCAACGCAGCATTTTGCCCAGTTACTCCAGAAGTACGGAATTGATACTGTCTTCGGCATAGTAGGTATTCCGATCGTGCAGTTGGCCGACACTATGGTCGCGAATGGAATTAAGTTTATACCGTGTCGCAATGAGCAAGCCGCAAGTTACGCGGCAAGCGCCTACGGCTACATCTCGGACAAACCTGGTGTCCTGTTAATCGTGGGAGGGCCTGGCCTTATTCATGCCTTAGCGGGGATCTATAATAGTATGTCGAACCGCTGGCCCTTGTTGGTCATCGCCGGATCCAGCAGCCAGTCAGACATCCACAAGGGAGGTTTCCAGGAACTTGACCAGGTGTCCTTGCTAAGTCCGTTCCTAAAATTCACTGGTAAACTGACGCCGGATAACATCGATATGATAACCCAAAAAGCACTCAACTATTGCATTCAGGGAACCGCCGGAGTGAGCTATATCGATGTCCCCGCTGACTTCATCGAATACGAGAAGCCGCTTGAGGGCAATGATAGAACTGGTAACGAGCTGCCAATGATCCTGACGCCCAATATCTGCGGTCCAGACCCCAGTAAAATTAAGAAGGTGGTGCAATTGATACTCCAGCACAAGAACAAGAACATATTAATAGTGATAGGTAAAGGAGCAGTGAAGAATAGCCATGAGATTAGACGCTTGGTTAACACCTTTAACTTGCCGTTCCTTCCGACTCCCATGGCGAAAGGCATAGTGCCCGATAGCAGCCCTTTAAATGTGAGCAGCGCCCGGAGCCAGGCTCTTAAGATTGCTGACATCGTGTTAGTATTGGGAGCTCGCCTGAATTGGATCCTTCACTTCGGAACGAGTCCAAAGTGGAATAGCGAGAGTATTTTTATACAATTTGACAGTAACCCGGAAACGCTCGGAGACAATAACGTTAGTCCCGGAGCAGATTTAAGTATCTGGGGGGACATCGGACTATCGGTTACTGCACTGGTAGAAGAGCTCACGAGACAGGACAGTTGTTGGAAATACTCGGGAGTCAAACAGGAGATCAGGGAAAAGATACAGTTAAACCAGACCAGATTGCTCCGTAAGGAAAAAACGCGTGGAGCTCAGCTTAACTATAATCAAGTGTACGGCACCCTTCGTCCGTTAATTGACGACTATCGCACGATCTTGGTCACAGAAGGTGCCAACACAATGGACATCGCTAGAATAAGTTTTCCGACTGATGCACCTCGCAGAAGACTTGATGCTGGTACAAATGCTACAATGGGTATAGGTTTAGGTTACGCATTAGCGTGTAAAGCCAGCCACCCTGAGTTAGACGTTGTTTTAATCCAAGGCGACAGTGCCTTCGGTTTTAGCGCTATGGAGATCGAGACTGCCGTCCGTTGCCAATTAGCCCTTGTGATTGTCGTAATGAATAACTCTGGGATATATCACGGTGAGAAAGACATCGAAGGAGATCTGCCCCCGACCGCTTTGTCAAAAAATTGTAGATATGACTTGGTCGGTAAGGGTTTAGGAGCTAACGACTTCTTCGTCAATACCATCTCAGAGTTATCTCGCTGTTTTCAGCAGGCAGTTCAATTGAGCAGAACCAAGCGCGAAACCTCCGTCATTAATGTCATAATCGAACCGGGAGAGCAGAAACAGATCGCGTTCGCATGGCAAAACAAGCCTAGACTG
SEQ ID NO:162(核糖体结合位点RBS3b和Sc_YEL020C(密码子优化的)开放读码框)：
GCATCTACCCCTAACAACCCATAAATTTAACTTTAAAAAATTTTTAAATATAAGAAAATGACTACCACAGCAACGCAGCATTTTGCCCAGTTACTCCAGAAGTACGGAATTGATACTGTCTTCGGCATAGTAGGTATTCCGATCGTGCAGTTGGCCGACACTATGGTCGCGAATGGAATTAAGTTTATACCGTGTCGCAATGAGCAAGCCGCAAGTTACGCGGCAAGCGCCTACGGCTACATCTCGGACAAACCTGGTGTCCTGTTAATCGTGGGAGGGCCTGGCCTTATTCATGCCTTAGCGGGGATCTATAATAGTATGTCGAACCGCTGGCCCTTGTTGGTCATCGCCGGATCCAGCAGCCAGTCAGACATCCACAAGGGAGGTTTCCAGGAACTTGACCAGGTGTCCTTGCTAAGTCCGTTCCTAAAATTCACTGGTAAACTGACGCCGGATAACATCGATATGATAACCCAAAAAGCACTCAACTATTGCATTCAGGGAACCGCCGGAGTGAGCTATATCGATGTCCCCGCTGACTTCATCGAATACGAGAAGCCGCTTGAGGGCAATGATAGAACTGGTAACGAGCTGCCAATGATCCTGACGCCCAATATCTGCGGTCCAGACCCCAGTAAAATTAAGAAGGTGGTGCAATTGATACTCCAGCACAAGAACAAGAACATATTAATAGTGATAGGTAAAGGAGCAGTGAAGAATAGCCATGAGATTAGACGCTTGGTTAACACCTTTAACTTGCCGTTCCTTCCGACTCCCATGGCGAAAGGCATAGTGCCCGATAGCAGCCCTTTAAATGTGAGCAGCGCCCGGAGCCAGGCTCTTAAGATTGCTGACATCGTGTTAGTATTGGGAGCTCGCCTGAATTGGATCCTTCACTTCGGAACGAGTCCAAAGTGGAATAGCGAGAGTATTTTTATACAATTTGACAGTAACCCGGAAACGCTCGGAGACAATAACGTTAGTCCCGGAGCAGATTTAAGTATCTGGGGGGACATCGGACTATCGGTTACTGCACTGGTAGAAGAGCTCACGAGACAGGACAGTTGTTGGAAATACTCGGGAGTCAAACAGGAGATCAGGGAAAAGATACAGTTAAACCAGACCAGATTGCTCCGTAAGGAAAAAACGCGTGGAGCTCAGCTTAACTATAATCAAGTGTACGGCACCCTTCGTCCGTTAATTGACGACTATCGCACGATCTTGGTCACAGAAGGTGCCAACACAATGGACATCGCTAGAATAAGTTTTCCGACTGATGCACCTCGCAGAAGACTTGATGCTGGTACAAATGCTACAATGGGTATAGGTTTAGGTTACGCATTAGCGTGTAAAGCCAGCCACCCTGAGTTAGACGTTGTTTTAATCCAAGGCGACAGTGCCTTCGGTTTTAGCGCTATGGAGATCGAGACTGCCGTCCGTTGCCAATTAGCCCTTGTGATTGTCGTAATGAATAACTCTGGGATATATCACGGTGAGAAAGACATCGAAGGAGATCTGCCCCCGACCGCTTTGTCAAAAAATTGTAGATATGACTTGGTCGGTAAGGGTTTAGGAGCTAACGACTTCTTCGTCAATACCATCTCAGAGTTATCTCGCTGTTTTCAGCAGGCAGTTCAATTGAGCAGAACCAAGCGCGAAACCTCCGTCATTAATGTCATAATCGAACCGGGAGAGCAGAAACAGATCGCGTTCGCATGGCAAAACAAGCCTAGACTG
SEQ ID NO:163(来自酿酒酵母S288C的草酰辅酶A脱羧酶(OXC)；EC 4.1.1.8；UniProt Accession P39994；Sc_YEL020C；密码子优化的)：
ATGACTACCACAGCAACGCAGCATTTTGCCCAGTTACTCCAGAAGTACGGAATTGATACTGTCTTCGGCATAGTAGGTATTCCGATCGTGCAGTTGGCCGACACTATGGTCGCGAATGGAATTAAGTTTATACCGTGTCGCAATGAGCAAGCCGCAAGTTACGCGGCAAGCGCCTACGGCTACATCTCGGACAAACCTGGTGTCCTGTTAATCGTGGGAGGGCCTGGCCTTATTCATGCCTTAGCGGGGATCTATAATAGTATGTCGAACCGCTGGCCCTTGTTGGTCATCGCCGGATCCAGCAGCCAGTCAGACATCCACAAGGGAGGTTTCCAGGAACTTGACCAGGTGTCCTTGCTAAGTCCGTTCCTAAAATTCACTGGTAAACTGACGCCGGATAACATCGATATGATAACCCAAAAAGCACTCAACTATTGCATTCAGGGAACCGCCGGAGTGAGCTATATCGATGTCCCCGCTGACTTCATCGAATACGAGAAGCCGCTTGAGGGCAATGATAGAACTGGTAACGAGCTGCCAATGATCCTGACGCCCAATATCTGCGGTCCAGACCCCAGTAAAATTAAGAAGGTGGTGCAATTGATACTCCAGCACAAGAACAAGAACATATTAATAGTGATAGGTAAAGGAGCAGTGAAGAATAGCCATGAGATTAGACGCTTGGTTAACACCTTTAACTTGCCGTTCCTTCCGACTCCCATGGCGAAAGGCATAGTGCCCGATAGCAGCCCTTTAAATGTGAGCAGCGCCCGGAGCCAGGCTCTTAAGATTGCTGACATCGTGTTAGTATTGGGAGCTCGCCTGAATTGGATCCTTCACTTCGGAACGAGTCCAAAGTGGAATAGCGAGAGTATTTTTATACAATTTGACAGTAACCCGGAAACGCTCGGAGACAATAACGTTAGTCCCGGAGCAGATTTAAGTATCTGGGGGGACATCGGACTATCGGTTACTGCACTGGTAGAAGAGCTCACGAGACAGGACAGTTGTTGGAAATACTCGGGAGTCAAACAGGAGATCAGGGAAAAGATACAGTTAAACCAGACCAGATTGCTCCGTAAGGAAAAAACGCGTGGAGCTCAGCTTAACTATAATCAAGTGTACGGCACCCTTCGTCCGTTAATTGACGACTATCGCACGATCTTGGTCACAGAAGGTGCCAACACAATGGACATCGCTAGAATAAGTTTTCCGACTGATGCACCTCGCAGAAGACTTGATGCTGGTACAAATGCTACAATGGGTATAGGTTTAGGTTACGCATTAGCGTGTAAAGCCAGCCACCCTGAGTTAGACGTTGTTTTAATCCAAGGCGACAGTGCCTTCGGTTTTAGCGCTATGGAGATCGAGACTGCCGTCCGTTGCCAATTAGCCCTTGTGATTGTCGTAATGAATAACTCTGGGATATATCACGGTGAGAAAGACATCGAAGGAGATCTGCCCCCGACCGCTTTGTCAAAAAATTGTAGATATGACTTGGTCGGTAAGGGTTTAGGAGCTAACGACTTCTTCGTCAATACCATCTCAGAGTTATCTCGCTGTTTTCAGCAGGCAGTTCAATTGAGCAGAACCAAGCGCGAAACCTCCGTCATTAATGTCATAATCGAACCGGGAGAGCAGAAACAGATCGCGTTCGCATGGCAAAACAAGCCTAGACTG
SEQ ID NO:164(核糖体结合位点)：
GCATCTAAACTAAAATACAATAACCAAAAATCATTACAAATGAAAAAAAATAATAAA
SEQ ID NO:165(核糖体结合位点)：
GCATCTAAACTAAAATACAATAACCAAAAATCATGTTTAACAAATAAAAATAATAAA
SEQ ID NO:166(核糖体结合位点)：
GCATCTAAACTAAAATACAATAACCAAAAATCATCTATGCGTAAAAAAAATTATAAA
SEQ ID NO:167(核糖体结合位点)：
GCATCTAAACTAAAATACAATAACCAAAAATCATACTACATGTATAAAATTAAAAAA
SEQ ID NO:168(核糖体结合位点)：
GCATCTAAACTAAAATACAATAACCAAAAATCATTTTCACAATATAAAAATAACAAA
SEQ ID NO:169(核糖体结合位点)：
GCATCATCTACCTCAATTATTCACCATTCCATCTGTCTAGCCAAAAAATTTAAAAAA
SEQ ID NO:170(核糖体结合位点)：
GCATCATCTACCTAAATTACTCATCATCCCATTTTAGTCAGCTTAAAATATTATAAA
SEQ ID NO:171(核糖体结合位点)：
GCATCATCTACCTATATTATTTATCATTCCATATCATGCGTGAATAAAAATAATAAA
SEQ ID NO:172(核糖体结合位点)：
GCATCATCTACCTATATTAATCATCAACCCAATTTTCATATGTATAAAATTAACAAA
SEQ ID NO:173(核糖体结合位点)：
GCATCTTTTCCATAACAAAAATTAAACCTCCTCCATATGAAAAATAAAAATTATAAA
SEQ ID NO:174(核糖体结合位点)：
GCATCAACTACCTCAATTAATAATCAAACCATCTGAACAATGAATAAATTTAAAAAA
SEQ ID NO:175(核糖体结合位点)：
GCATCTTTTCCATAACAAAAATTAAACCTCCTCCATATGAGATATAAAATTAACAAA
SEQ ID NO:176(核糖体结合位点)：
GCATCAACTACCTTAATTATTCATCAAACCATATAACATACTAAAAAAAATAACAAA
SEQ ID NO:177(核糖体结合位点)：
GCATCATCTACCTATATTACTAATCATTCCAATTAATTACCTTATAAAATTAAGAAA
SEQ ID NO:178(核糖体结合位点)：
GCATCTTTTCCATAACAAAAATTAAACCTCCTCCCCCCCACGTTTAAAATTAAAAAA
SEQ ID NO:179(核糖体结合位点)：
GCATCAACTACCTTCATTACTTAACAATCCACCTGTTGTGAGTAAAAATATAAGAAA
SEQ ID NO:180(核糖体结合位点)：
GCATCCCCAAAAAATTAATTCATTTTCTCTTTTCCCTAACCCTTAAAATATAATAAA
SEQ ID NO:181(核糖体结合位点)：
GCATCAACTACCTAAATTAATTATCACCCCAAATTAATAACATAAAAATATAATAAA
SEQ ID NO:182(核糖体结合位点)：
GCATCAACTACCTAAATTAATTATCACCCCAAATTAATAACATAAAAATATAATAAA
SEQ ID NO:183(核糖体结合位点)：
GCATCCCCAAAAAATTAATTCATTTTCTCTTTTCAACAATTTAAAAAAAATTAAAAA
SEQ ID NO:184(核糖体结合位点)：
GCATCCCCAAAAAATTAATTCATTTTCTCTTTTCGATCCTATAAAAAATATAACAAA
SEQ ID NO:185(核糖体结合位点)：
GCATCATCTACCTTTATTATTAATCAATCCATCTCAGTAAGTTTAAAATATAACAAA
SEQ ID NO:186(核糖体结合位点)：
GCATCAACTACCTCTATTAATAATCAATCCACATCTTGATCGTAAAAAATTTAAAAA
SEQ ID NO:187(核糖体结合位点)：
GCATCACCTACCTAAATTACTAAACAAACCACCTGCATTTGTTTTAAAAATTACAAA
SEQ ID NO:188(核糖体结合位点)：
GCATCAACTACCTAAATTAATCATCAAACCAACTAGAATATGAAAAAAAATAAAAAA
SEQ ID NO:189(核糖体结合位点)：
GCATCAACTACCTCAATTAATAAACAAACCAACTGTCCAATGTATAAAATTAAAAAA
SEQ ID NO:190(核糖体结合位点)：
GCATCAACTACCTCAATTACTAAACAATCCACATTATTAATATAAAAATATAAAAAA
SEQ ID NO:191(核糖体结合位点)：
GCATCAACACCTAAATTAATTAACACACCATATATTAAACCATAAAAAATAAGAAA
SEQ ID NO:192(核糖体结合位点)：
GCATCTTCTACCCATATTCCCCCTAATCCCATCTGTTATATTATTAAATTTAATAAA
SEQ ID NO:193(核糖体结合位点)：
GCATCATCTACCTACATTATTTAACAAACCACATTTAAAAGGAAAAAATATTAAAAA
SEQ ID NO:194(核糖体结合位点)：
GCATCACCTACCTATATTATTTATCATTCCACATGCGTTATTATTAAATTTTAAAAA
SEQ ID NO:195(核糖体结合位点)：
GCATCTAAACAACATATTCCCCCTAAACCAAAATATTTAGGGTATAAAAATTAAAAA
SEQ ID NO:196(核糖体结合位点)：
GCATCATCTACCTTTATTAATCAACAATCCAAATTATTAATAAAAAAAATTTAAAAA
SEQ ID NO:197(核糖体结合位点)：
GCATCAACTACCTAAATTACTTATCAAACCACTTAGTAGTAAAAAAAATATAACAAA
SEQ ID NO:198(核糖体结合位点)：
GCATCACTATTACAAAAAAAACACCAATATTACATGGTCTCTTTTAAATATTATAAA
SEQ ID NO:199(核糖体结合位点)：
GCATCATCTACCTATATTACTAAACAAACCAATTATAATAATTTTAAAAATAAAAAA
SEQ ID NO:200(核糖体结合位点)：
GCATCACCTACCTCTATTACTAATCAAACCATTTAAATGTAGTAAAAATATAATAAA
SEQ ID NO:201(核糖体结合位点)：
GCATCAACTACCTAAATTAATAAACACACCAACTTCCATGAATAAAAATATAAAAAA
SEQ ID NO:202(核糖体结合位点)：
GCATCATCTACCTATATTATTTACCACCCCACCTAACTTTAGATTAAAATTTACAAA
SEQ ID NO:203(核糖体结合位点)：
GCATCACCTACCTTCATTACTCACCATACCAACTAGCACAGTTATAAAAATAAGAAA
SEQ ID NO:204(核糖体结合位点)：
GCATCATCTACCTAAATTAATAAACAACCCATTTATAAACCCTTTAAAATTAACAAA
SEQ ID NO:205(核糖体结合位点)：
GCATCTTAATCAATTTTAAACTCACCAAATCAAAATATGAAAAAAAAAAATAATAAA
SEQ ID NO:206(核糖体结合位点)：
GCATCATCTACCTTAATTAATAAACAAACCAATTTAACTAATTTAAAAAATATAAA
SEQ ID NO:207(核糖体结合位点)：
GCATCATCTACCTTCATTATTAATCATTCCAATTGTATCACTTAAAAATATAAAAAA
SEQ ID NO:208(核糖体结合位点)：
GCATCTTAATCAATTTTAAACTCACCAAATCAAATATCACGTAAAAAATATAAGAAA
SEQ ID NO:209(核糖体结合位点)：
GCATCAACTACCTCAATTATTTAACACACCAATTACTATACGTAAAAATATAAAAAA
SEQ ID NO:210(核糖体结合位点)：
GCATCTTAATCAATTTTAAACTCACCAAATCAAAAAAATGCGTATAAAAATTAAAAA
SEQ ID NO:211(核糖体结合位点)：
GCATCTTAATCAATTTTAAACTCACCAAATCAAAGGCCGACTTAAAAATATTAGAAA
SEQ ID NO:212(核糖体结合位点)：
GCATCACCTAAAAATTATAAATAAATTAATAAACCTTTTAACATAAAAATTAATAAA
SEQ ID NO:213(核糖体结合位点)：
GCATCACCTAAAAATTATAAATAAATTAATAAACTTTTATTAAAAAAAAATTACAAA
SEQ ID NO:214(核糖体结合位点)：
GCATCACCTAAAAATTATAAATAAATTAATAAACATAACATAAATAAAATTAATAAA
SEQ ID NO:215(核糖体结合位点)：
GCATCACCTAAAAATTATAAATAAATTAATAAACCACAATACTAAAAATTTAATAAA
SEQ ID NO:216(核糖体结合位点)：
GCATCAACTACCTACATTAATAACCAACCCAAATGCCTAATCAATAAATATTATAAA
SEQ ID NO:217(核糖体结合位点)：
GCATCATCTACCTATATTATTCATCAAACCATTTGATCCAACATTAAATATTAAAAA
SEQ ID NO:218(核糖体结合位点)：
GCATCACCTACCTCAATTATTAATCATACCAACTGGATTTGATTTAAAAATAAGAAA
SEQ ID NO:219(核糖体结合位点)：
GCATCACCTACCTTTATTACTCATCATACCAAATGGTAGCGGATTAAATATTAGAAA
SEQ ID NO:220(核糖体结合位点)：
GCATCATCTACCTTAATTAATCAACAAACCAATTACCAACCTAAAAAATATAAGAAA
SEQ ID NO:221(核糖体结合位点)：
GCATCAACTACCTTTATTATTCAACAAACCAATTTAACATATTAAAAAAATAAAAAA
SEQ ID NO:222(核糖体结合位点)：
GCATCTACCCCTAACAACCCATAAATTTAACTTTAAAAAATTTTTAAATATAATAAA
SEQ ID NO:223(核糖体结合位点)：
GCATCATCTACCTTCATTATTAAACAAACCAAATTAATATCATAAAAAAATAAAAAA
SEQ ID NO:224(核糖体结合位点)：
GCATCTACCCCTAACAACCCATAAATTTAACTTTTCTACAAAAAAAAATATAAAAAA
SEQ ID NO:225(核糖体结合位点)：
GCATCACCTACCTACATTAATAACCATACCATCTCAGTTTTAAATAAATTTTAAAAA
SEQ ID NO:226(核糖体结合位点)：
GCATCACCTACCTTAATTAATCACCATTCCATATCCTTTCAAAAAAAATATAAAAAA
SEQ ID NO:227(核糖体结合位点)：
GCATCACCTACCTCTATTAATCATCATTCCAATTTAGGCTCATTAAATATTAGAAA
SEQ ID NO:228(核糖体结合位点)：
GCATCATCTACCTTAATTAATCAACAAACCAATTACCAACCTAAAAAATATAAGAAA
SEQ ID NO:229(核糖体结合位点)：
GCATCAACTACCTTTATTATTCAACAAACCAATTTAACATAGTAAAAAAATAAAAAA
SEQ ID NO:230(核糖体结合位点)：
GCATCTACCCCTAACAACCCATAAATTTAACTTTAAAAAATTTTTAAATATAAGAAA
Claims (64)
1.一种共生细菌，其与未经修饰的类似的或在其它方面相同的细菌相比已被修饰成具有增加的草酸盐降解活性。
2.权利要求1所述的细菌，其中所述细菌属于选自以下的属：拟杆菌属(Bacteroides)、另枝菌属(Alistipes)、栖粪杆菌属(Faecalibacterium)、副拟杆菌属(Parabacteroides)、普雷沃氏菌属(Prevotella)、罗氏菌属(Roseburia)、瘤胃球菌属(Ruminococcus)、梭菌属(Clostridium)、颤杆菌属(Oscillibacter)、芽殖菌属(Gemmiger)、巴恩斯氏菌(Barnesiella)、戴阿利斯特杆菌属(Dialister)、副萨特氏菌属(Parasutterella)、考拉杆菌属(Phascolarctobacterium)、丙酸杆菌属(Propionibacterium)、萨特氏菌属(Sutterella)、布劳特氏菌属(Blautia)、副普雷沃氏菌属(Paraprevotella)、粪球菌属(Coprococcus)、臭菌属(Odoribacter)、螺原体属(Spiroplasma)、厌氧棒状菌属(Anaerostipes)和阿克曼氏菌属(Akkermansia)。
3.权利要求2所述的细菌，其中所述属是拟杆菌属。
4.权利要求1-3中任一项所述的细菌，其中所述未经修饰的类似的或在其它方面相同的细菌不具有可检测到的草酸盐降解活性。
5.权利要求1-4中任一项所述的细菌，其中所述未经修饰的类似的或在其它方面相同的细菌不具有已知参与草酸盐分解代谢的基因。
6.权利要求1-5中任一项所述的细菌，其中与所述细菌相同属的天然存在的成员不具有可检测到的草酸盐降解活性。
7.权利要求1-6中任一项所述的细菌，其中所述细菌包含一种或多种编码蛋白或其功能片段或变体的转基因，所述蛋白选自：
(i)草酸盐:甲酸盐反向转运蛋白(OxlT)；
(ii)草酸脱羧酶(OXDC)-EC 4.1.1.2；
(iii)草酸氧化酶(OXO)-EC 1.2.3.4；
(iv)草酸氧化还原酶(OOR)-EC 1.2.7.10；
(v)草酸辅酶A连接酶/草酰辅酶A合成酶(OXS)-EC 6.2.1.8；
(vi)甲酰辅酶A:草酸辅酶A转移酶(FCOCT)-EC 2.8.3.16；
(vii)乙酰辅酶A:草酸辅酶A转移酶(ACOCT)-EC 2.8.3.19；
(viii)琥珀酰辅酶A:草酸辅酶A转移酶(SCOCT)-EC 2.8.3.2；
(ix)草酰辅酶A脱羧酶(OXC)-EC 4.1.1.8；
(x)草酰辅酶A还原酶/乙醛酸盐:NADP+氧化还原酶(OXR)-EC 1.2.1.17；和
(xi)任何前述蛋白的组合。
8.权利要求7所述的细菌，其中所述细菌包含一种或多种编码OxIT或其功能片段或变体、OXS或其功能片段或变体、和OXC或其功能片段或变体的转基因。
9.权利要求8所述的细菌，其中所述细菌进一步包含一种或多种编码FCOCT或其功能片段或变体的转基因。
10.权利要求8或9所述的细菌，其中所述细菌进一步包含一种或多种编码ACOCT或其功能片段或变体的转基因。
11.权利要求7所述的细菌，其中所述细菌包含一种或多种编码OxIT或其功能片段或变体、OXS或其功能片段或变体、第一OXC或其功能片段或变体、第二OXC或其功能片段或变体、FCOCT或其功能片段或变体、和ACOCT或其功能片段或变体的转基因。
12.权利要求7所述的细菌，其中所述细菌包含一种或多种编码OxIT或其功能片段或变体、第一OXS或其功能片段或变体、第二OXS或其功能片段或变体、OXC或其功能片段或变体、FCOCT或其功能片段或变体、和ACOCT或其功能片段或变体的转基因。
13.权利要求7所述的细菌，其中所述细菌包含一种或多种编码OxIT或其功能片段或变体、第一OXS或其功能片段或变体、第二OXS或其功能片段或变体、第一OXC或其功能片段或变体、第二OXC或其功能片段或变体、FCOCT或其功能片段或变体、和ACOCT或其功能片段或变体的转基因。
14.权利要求7-13中任一项所述的细菌，其中：
(i)所述OxIT是产甲酸草酸杆菌(O.formigenes)OxIT；
(ii)所述OXS是酿酒酵母(S.cerevisiae)或拟南芥(A.thaliana)OXS；
(iii)所述OXC是酿酒酵母或产甲酸草酸杆菌OXC；
(iv)所述FCOCT是大肠杆菌(E.coli)FCOCT；和/或
(v)所述ACOCT是大肠杆菌ACOCT。
15.权利要求14所述的细菌，其中所述细菌包含一种或多种编码酿酒酵母OXS、酿酒酵母OXC和产甲酸草酸杆菌OxIT的转基因。
16.权利要求14所述的细菌，其中所述细菌包含一种或多种编码大肠杆菌FCOCT、产甲酸草酸杆菌OXC、酿酒酵母OXS和产甲酸草酸杆菌OxIT的转基因。
17.权利要求14所述的细菌，其中所述细菌包含一种或多种编码大肠杆菌FCOCT、产甲酸草酸杆菌OXC、酿酒酵母OXS、酿酒酵母OXC、大肠杆菌ACOCT和产甲酸草酸杆菌OxIT的转基因。
18.权利要求14所述的细菌，其中所述细菌包含一种或多种编码大肠杆菌FCOCT、产甲酸草酸杆菌OXC、酿酒酵母OXS、酿酒酵母OXC、拟南芥OXS、大肠杆菌ACOCT和产甲酸草酸杆菌OxIT的转基因。
19.权利要求14所述的细菌，其中所述细菌包含一种或多种编码大肠杆菌FCOCT、产甲酸草酸杆菌OXC、产甲酸草酸杆菌OxIT、大肠杆菌ACOCT和酿酒酵母OXS的转基因。
20.权利要求14所述的细菌，其中所述细菌包含一种或多种编码产甲酸草酸杆菌OxIT、大肠杆菌FCOCT、酿酒酵母OXC、大肠杆菌ACOCT、酿酒酵母OXS和拟南芥OXS的转基因。
21.权利要求14所述的细菌，其中所述细菌包含一种或多种编码产甲酸草酸杆菌OxIT、大肠杆菌FCOCT、酿酒酵母OXC、大肠杆菌ACOCT和酿酒酵母OXS的转基因。
22.权利要求1-21中任一项所述的细菌，其中所述一种或多种转基因是在质粒上、在细菌人工染色体上或整合在基因组中。
23.权利要求1-22中任一项所述的细菌，其中所述细菌包含一种或多种核酸，所述核酸包含与SEQ ID NO:1-31中任一个具有至少80％同一性的核苷酸序列。
24.权利要求23所述的细菌，其中所述细菌包含一种或多种核酸，所述核酸包含与SEQID NO:16具有至少80％同一性的核苷酸序列、与SEQ ID NO:26或SEQ ID NO:163具有至少80％同一性的核苷酸序列和与SEQ ID NO:21具有至少80％同一性的核苷酸序列。
25.权利要求23所述的细菌，其中所述细菌包含一种或多种核酸，所述核酸包含与SEQID NO:7具有至少80％同一性的核苷酸序列、与SEQ ID NO:25具有至少80％同一性的核苷酸序列、与SEQ ID NO:16具有至少80％同一性的核苷酸序列和与SEQ ID NO:21具有至少80％同一性的核苷酸序列。
26.权利要求23所述的细菌，其中所述细菌包含一种或多种核酸，所述核酸包含与SEQID NO:7具有至少80％同一性的核苷酸序列、与SEQ ID NO:25具有至少80％同一性的核苷酸序列、与SEQ ID NO:16具有至少80％同一性的核苷酸序列、与SEQ ID NO:26或SEQ IDNO:163具有至少80％同一性的核苷酸序列、与SEQ ID NO:2具有至少80％同一性的核苷酸序列和与SEQ ID NO:21具有至少80％同一性的核苷酸序列。
27.权利要求23所述的细菌，其中所述细菌包含一种或多种核酸，所述核酸包含与SEQID NO:7具有至少80％同一性的核苷酸序列、与SEQ ID NO:25具有至少80％同一性的核苷酸序列、与SEQ ID NO:16具有至少80％同一性的核苷酸序列、与SEQ ID NO:26或SEQ IDNO:163具有至少80％同一性的核苷酸序列、与SEQ ID NO:14具有至少80％同一性的核苷酸序列、与SEQ ID NO:2具有至少80％同一性的核苷酸序列和与SEQ ID NO:21具有至少80％同一性的核苷酸序列。
28.权利要求23所述的细菌，其中所述细菌包含一种或多种核酸，所述核酸包含与SEQID NO:7具有至少80％同一性的核苷酸序列、与SEQ ID NO:25具有至少80％同一性的核苷酸序列、与SEQ ID NO:21具有至少80％同一性的核苷酸序列、与SEQ ID NO:2具有至少80％同一性的核苷酸序列和与SEQ ID NO:16具有至少80％同一性的核苷酸序列。
29.权利要求23所述的细菌，其中所述细菌包含一种或多种核酸，所述核酸包含与SEQID NO:21具有至少80％同一性的核苷酸序列、与SEQ ID NO:7具有至少80％同一性的核苷酸序列、与SEQ ID NO:26或SEQ ID NO:163具有至少80％同一性的核苷酸序列、与SEQ IDNO:2具有至少80％同一性的核苷酸序列、与SEQ ID NO:16具有至少80％同一性的核苷酸序列和与SEQ ID NO:14具有至少80％同一性的核苷酸序列。
30.权利要求23所述的细菌，其中所述细菌包含一种或多种核酸，所述核酸包含与SEQID NO:21具有至少80％同一性的核苷酸序列、与SEQ ID NO:7具有至少80％同一性的核苷酸序列、与SEQ ID NO:26或SEQ ID NO:163具有至少80％同一性的核苷酸序列、与SEQ IDNO:2具有至少80％同一性的核苷酸序列和与SEQ ID NO:16具有至少80％同一性的核苷酸序列。
31.权利要求7-30中任一项所述的细菌，其中所述一种或多种转基因中的至少一种可操作地连接至核糖体结合位点(RBS)。
32.权利要求31所述的细菌，其中所述RBS包含SEQ ID NO:164-230中任一个的核苷酸序列。
33.权利要求32所述的细菌，其中：
(i)所述细菌包含编码产甲酸草酸杆菌OxIT的转基因，所述转基因可操作地连接至包含SEQ ID NO:212-219中任一个的核苷酸序列的RBS；
(ii)所述细菌包含编码酿酒酵母OXS的转基因，所述转基因可操作地连接至包含SEQID NO:172-179中任一个的核苷酸序列的RBS；
(iii)所述细菌包含编码拟南芥OXS的转基因，所述转基因可操作地连接至包含SEQ IDNO:164-171中任一个的核苷酸序列的RBS；
(iv)所述细菌包含编码酿酒酵母OXC的转基因，所述转基因可操作地连接至包含SEQID NO:188-195或220-230中任一个的核苷酸序列的RBS；
(v)所述细菌包含编码产甲酸草酸杆菌OXC的转基因，所述转基因可操作地连接至包含SEQ ID NO:180-187中任一个的核苷酸序列的RBS；
(vi)所述细菌包含编码大肠杆菌FCOCT的转基因，所述转基因可操作地连接至包含SEQID NO:204-211中任一个的核苷酸序列的RBS；和/或
(vii)所述细菌包含编码大肠杆菌ACOCT的转基因，所述转基因可操作地连接至包含SEQ ID NO:196-203中任一个的核苷酸序列的RBS。
34.权利要求1-33中任一项所述的细菌，其中所述细菌包含一种或多种核酸，所述核酸包含与SEQ ID NO:96-162中任一个具有至少80％同一性的核苷酸序列。
35.权利要求34所述的细菌，其中
(i)所述细菌包含一种或多种核酸，所述核酸包含与SEQ ID NO:137具有至少80％同一性的核苷酸序列、与SEQ ID NO:113具有至少80％同一性的核苷酸序列、与SEQ ID NO:145具有至少80％同一性的核苷酸序列、与SEQ ID NO:128具有至少80％同一性的核苷酸序列和与SEQ ID NO:104具有至少80％同一性的核苷酸序列；
(ii)所述细菌包含一种或多种核酸，所述核酸包含与SEQ ID NO:155具有至少80％同一性的核苷酸序列、与SEQ ID NO:137具有至少80％同一性的核苷酸序列、与SEQ ID NO:128具有至少80％同一性的核苷酸序列、与SEQ ID NO:148具有至少80％同一性的核苷酸序列、与SEQ ID NO:115具有至少80％同一性的核苷酸序列、与SEQ ID NO:98具有至少80％同一性的核苷酸序列和与SEQ ID NO:105具有至少80％同一性的核苷酸序列；
(iii)所述细菌包含一种或多种核酸，所述核酸包含与SEQ ID NO:155具有至少80％同一性的核苷酸序列、与SEQ ID NO:137具有至少80％同一性的核苷酸序列、与SEQ ID NO:128具有至少80％同一性的核苷酸序列、与SEQ ID NO:144具有至少80％同一性的核苷酸序列、与SEQ ID NO:115具有至少80％同一性的核苷酸序列、与SEQ ID NO:98具有至少80％同一性的核苷酸序列和与SEQ ID NO:105具有至少80％同一性的核苷酸序列；
(iv)所述细菌包含一种或多种核酸，所述核酸包含与SEQ ID NO:147具有至少80％同一性的核苷酸序列、与SEQ ID NO:104具有至少80％同一性的核苷酸序列、与SEQ ID NO:153具有至少80％同一性的核苷酸序列、与SEQ ID NO:97具有至少80％同一性的核苷酸序列、与SEQ ID NO:131具有至少80％同一性的核苷酸序列、与SEQ ID NO:136具有至少80％同一性的核苷酸序列和与SEQ ID NO:113具有至少80％同一性的核苷酸序列；
(v)所述细菌包含一种或多种核酸，所述核酸包含与SEQ ID NO:145具有至少80％同一性的核苷酸序列、与SEQ ID NO:137具有至少80％同一性的核苷酸序列、与SEQ ID NO:153具有至少80％同一性的核苷酸序列、与SEQ ID NO:129具有至少80％同一性的核苷酸序列、与SEQ ID NO:105具有至少80％同一性的核苷酸序列、与SEQ ID NO:113具有至少80％同一性的核苷酸序列和与SEQ ID NO:96具有至少80％同一性的核苷酸序列；
(vi)所述细菌包含一种或多种核酸，所述核酸包含与SEQ ID NO:145具有至少80％同一性的核苷酸序列、与SEQ ID NO:137具有至少80％同一性的核苷酸序列、与SEQ ID NO:153具有至少80％同一性的核苷酸序列、与SEQ ID NO:129具有至少80％同一性的核苷酸序列、与SEQ ID NO:105具有至少80％同一性的核苷酸序列和与SEQ ID NO:96具有至少80％同一性的核苷酸序列；
(vii)所述细菌包含一种或多种核酸，所述核酸包含与SEQ ID NO:139具有至少80％同一性的核苷酸序列、与SEQ ID NO:129具有至少80％同一性的核苷酸序列、与SEQ ID NO:155具有至少80％同一性的核苷酸序列、与SEQ ID NO:112具有至少80％同一性的核苷酸序列、与SEQ ID NO:99具有至少80％同一性的核苷酸序列、与SEQ ID NO:106具有至少80％同一性的核苷酸序列和与SEQ ID NO:145具有至少80％同一性的核苷酸序列；
(viii)所述细菌包含一种或多种核酸，所述核酸包含与SEQ ID NO:139具有至少80％同一性的核苷酸序列、与SEQ ID NO:154具有至少80％同一性的核苷酸序列、与SEQ ID NO:131具有至少80％同一性的核苷酸序列、与SEQ ID NO:98具有至少80％同一性的核苷酸序列、与SEQ ID NO:115具有至少80％同一性的核苷酸序列、与SEQ ID NO:106具有至少80％同一性的核苷酸序列和与SEQ ID NO:149具有至少80％同一性的核苷酸序列；
(ix)所述细菌包含一种或多种核酸，所述核酸包含与SEQ ID NO:137具有至少80％同一性的核苷酸序列、与SEQ ID NO:113具有至少80％同一性的核苷酸序列、与SEQ ID NO:148具有至少80％同一性的核苷酸序列、与SEQ ID NO:129具有至少80％同一性的核苷酸序列和与SEQ ID NO:105具有至少80％同一性的核苷酸序列；
(x)所述细菌包含一种或多种核酸，所述核酸包含与SEQ ID NO:145具有至少80％同一性的核苷酸序列、与SEQ ID NO:137具有至少80％同一性的核苷酸序列、与SEQ ID NO:153具有至少80％同一性的核苷酸序列、与SEQ ID NO:129具有至少80％同一性的核苷酸序列和与SEQ ID NO:105具有至少80％同一性的核苷酸序列；
(xi)所述细菌包含一种或多种核酸，所述核酸包含与SEQ ID NO:155具有至少80％同一性的核苷酸序列、与SEQ ID NO:137具有至少80％同一性的核苷酸序列、与SEQ ID NO:128具有至少80％同一性的核苷酸序列、与SEQ ID NO:144具有至少80％同一性的核苷酸序列、与SEQ ID NO:115具有至少80％同一性的核苷酸序列、与SEQ ID NO:98具有至少80％同一性的核苷酸序列和与SEQ ID NO:105具有至少80％同一性的核苷酸序列；
(xii)所述细菌包含一种或多种核酸，所述核酸包含与SEQ ID NO:145具有至少80％同一性的核苷酸序列、与SEQ ID NO:137具有至少80％同一性的核苷酸序列、与SEQ ID NO:153具有至少80％同一性的核苷酸序列、与SEQ ID NO:129具有至少80％同一性的核苷酸序列、与SEQ ID NO:105具有至少80％同一性的核苷酸序列、与SEQ ID NO:113具有至少80％同一性的核苷酸序列和与SEQ ID NO:96具有至少80％同一性的核苷酸序列；
(xiii)所述细菌包含一种或多种核酸，所述核酸包含与SEQ ID NO:145具有至少80％同一性的核苷酸序列、与SEQ ID NO:137具有至少80％同一性的核苷酸序列、与SEQ ID NO:153具有至少80％同一性的核苷酸序列、与SEQ ID NO:129具有至少80％同一性的核苷酸序列、与SEQ ID NO:105具有至少80％同一性的核苷酸序列和与SEQ ID NO:96具有至少80％同一性的核苷酸序列；或者
(xiv)所述细菌包含一种或多种核酸，所述核酸包含与SEQ ID NO:139具有至少80％同一性的核苷酸序列、与SEQ ID NO:129具有至少80％同一性的核苷酸序列、与SEQ ID NO:155具有至少80％同一性的核苷酸序列、与SEQ ID NO:112具有至少80％同一性的核苷酸序列、与SEQ ID NO:99具有至少80％同一性的核苷酸序列、与SEQ ID NO:106具有至少80％同一性的核苷酸序列和与SEQ ID NO:145具有至少80％同一性的核苷酸序列。
36.权利要求1-35中任一项所述的细菌，其中所述细菌包含一种或多种核酸，所述核酸包含与SEQ ID NO:82-95中任一个具有至少80％同一性的核苷酸序列。
37.权利要求7-36中任一项所述的细菌，其中所述一种或多种转基因中的至少一种可操作地连接至至少一个组成型启动子。
38.权利要求37所述的细菌，其中所述启动子是噬菌体衍生的启动子。
39.权利要求37或38所述的细菌，其中所述启动子包含共有序列GTTAA(n)4-7GTTAA(n)34-38TA(n)2TTTG(SEQ ID NO:70)。
40.权利要求37-39中任一项所述的细菌，其中所述启动子包含SEQ ID NO:68、SEQ IDNO:69或SEQ ID NO:71。
41.权利要求1-40中任一项所述的细菌，其中所述细菌已被修饰成以增加的丰度、稳定性、预测性或初始定殖的容易性定殖于人肠道。
42.权利要求41所述的细菌，其中所述细菌已被修饰以增加其利用特殊营养物作为碳源的能力。
43.权利要求42所述的细菌，其中所述细菌包含一种或多种增加其利用特殊营养物作为碳源的能力的转基因。
44.权利要求42或43所述的细菌，其中所述特殊营养物是海洋多糖。
45.权利要求44所述的细菌，其中所述海洋多糖是紫菜多糖。
46.权利要求41-45中任一项所述的细菌，其中，在施用给人受试者后，所述细菌的丰度大于1012、1011、1010、109、108或107cfu/克粪便含量。
47.一种药物组合物，其包含权利要求1-46中任一项所述的细菌和药学上可接受的赋形剂。
48.根据权利要求47所述的药物组合物，其中所述组合物被配制成胶囊或片剂。
49.根据权利要求48所述的药物组合物，其中所述胶囊是肠溶衣胶囊。
50.根据权利要求47-49中任一项所述的药物组合物，其中所述组合物进一步包含特殊营养物。
51.根据权利要求50所述的药物组合物，其中所述特殊营养物是海洋多糖。
52.根据权利要求51所述的药物组合物，其中所述海洋多糖是紫菜多糖。
53.一种降低受试者中的草酸盐水平的方法，所述方法包括向所述受试者施用权利要求1-46中任一项所述的细菌或权利要求47-52中任一项所述的药物组合物。
54.根据权利要求53所述的方法，其中所述受试者具有与草酸盐量升高相关的病症。
55.一种治疗受试者中与草酸盐量升高相关的病症的方法，所述方法包括向所述受试者施用权利要求1-46中任一项所述的细菌或权利要求47-52中任一项所述的药物组合物。
56.根据权利要求54或55所述的方法，其中所述病症是高草酸尿症。
57.根据权利要求53-56中任一项所述的方法，所述方法进一步包含向所述受试者施用特殊营养物。
58.根据权利要求57所述的方法，其中在所述细菌之前、同时或之后向所述受试者施用所述特殊营养物。
59.根据权利要求58所述的方法，其中所述特殊营养物是海洋多糖。
60.根据权利要求59所述的方法，其中所述海洋多糖是紫菜多糖。
61.根据权利要求53-60中任一项所述的方法，其中每12小时、24小时、1天、2天、3天、4天、5天、6天、7天、1周、2周、3周、4周、1个月、2个月、3个月、4个月、5个月或6个月向所述受试者施用所述细菌或药物组合物。
62.根据权利要求53-61中任一项所述的方法，其中向所述受试者连续施用所述细菌或药物组合物之间的时间大于48小时。
63.根据权利要求53-62中任一项所述的方法，其中所述受试者是动物。
64.根据权利要求63所述的方法，其中所述受试者是人。
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| PCT/US2019/065446 WO2020123483A1 (en) | 2018-12-10 | 2019-12-10 | Methods and compositions for treating hyperoxaluria |
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| CN1365284A (zh) * | 1999-05-28 | 2002-08-21 | Vsl制药有限公司 | 用于预防或治疗尿草酸盐过多的膳食或药物组合物 |
| WO2017040719A1 (en) * | 2015-08-31 | 2017-03-09 | Synlogic, Inc. | Bacteria engineered to treat disorders in which oxalate is detrimental |
| WO2018112194A1 (en) * | 2016-12-15 | 2018-06-21 | The Board Of Trustees Of The Leland Stanford Junior University | Compositions and methods for modulating growth of a genetically modified gut bacterial cell |
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| CN1365284A (zh) * | 1999-05-28 | 2002-08-21 | Vsl制药有限公司 | 用于预防或治疗尿草酸盐过多的膳食或药物组合物 |
| WO2017040719A1 (en) * | 2015-08-31 | 2017-03-09 | Synlogic, Inc. | Bacteria engineered to treat disorders in which oxalate is detrimental |
| US20180325963A1 (en) * | 2015-08-31 | 2018-11-15 | Synlogic, Inc. | Bacteria engineered to treat disorders in which oxalate is detrimental |
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| KR20210125988A (ko) | 2021-10-19 |
| AU2019396366A1 (en) | 2021-07-15 |
| JP2022512380A (ja) | 2022-02-03 |
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