CN113384496A - Synthesis process and application of schizosaccharomyces cerevisiae fermentation product lysate and carnosine composition - Google Patents
Synthesis process and application of schizosaccharomyces cerevisiae fermentation product lysate and carnosine composition Download PDFInfo
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- CN113384496A CN113384496A CN202110747941.1A CN202110747941A CN113384496A CN 113384496 A CN113384496 A CN 113384496A CN 202110747941 A CN202110747941 A CN 202110747941A CN 113384496 A CN113384496 A CN 113384496A
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- bacterial cellulose
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- 238000000855 fermentation Methods 0.000 title claims abstract description 56
- 230000004151 fermentation Effects 0.000 title claims abstract description 56
- CQOVPNPJLQNMDC-UHFFFAOYSA-N N-beta-alanyl-L-histidine Natural products NCCC(=O)NC(C(O)=O)CC1=CN=CN1 CQOVPNPJLQNMDC-UHFFFAOYSA-N 0.000 title claims abstract description 51
- CQOVPNPJLQNMDC-ZETCQYMHSA-N carnosine Chemical compound [NH3+]CCC(=O)N[C@H](C([O-])=O)CC1=CNC=N1 CQOVPNPJLQNMDC-ZETCQYMHSA-N 0.000 title claims abstract description 51
- 108010087806 Carnosine Proteins 0.000 title claims abstract description 50
- QRYRORQUOLYVBU-VBKZILBWSA-N Carnosic acid Natural products CC([C@@H]1CC2)(C)CCC[C@]1(C(O)=O)C1=C2C=C(C(C)C)C(O)=C1O QRYRORQUOLYVBU-VBKZILBWSA-N 0.000 title claims abstract description 40
- 229940044199 carnosine Drugs 0.000 title claims abstract description 40
- 239000000203 mixture Substances 0.000 title claims abstract description 39
- 239000006166 lysate Substances 0.000 title claims abstract description 38
- 238000000034 method Methods 0.000 title claims abstract description 27
- 230000008569 process Effects 0.000 title claims abstract description 25
- 241000235346 Schizosaccharomyces Species 0.000 title claims abstract description 13
- 230000015572 biosynthetic process Effects 0.000 title description 9
- 238000003786 synthesis reaction Methods 0.000 title description 9
- 229920002749 Bacterial cellulose Polymers 0.000 claims abstract description 52
- 239000005016 bacterial cellulose Substances 0.000 claims abstract description 52
- 240000004808 Saccharomyces cerevisiae Species 0.000 claims abstract description 44
- 238000002156 mixing Methods 0.000 claims abstract description 40
- 238000003756 stirring Methods 0.000 claims abstract description 35
- 238000001914 filtration Methods 0.000 claims abstract description 31
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 24
- 238000001035 drying Methods 0.000 claims abstract description 17
- 239000003054 catalyst Substances 0.000 claims abstract description 15
- 235000012424 soybean oil Nutrition 0.000 claims abstract description 11
- 239000003549 soybean oil Substances 0.000 claims abstract description 11
- IXPNQXFRVYWDDI-UHFFFAOYSA-N 1-methyl-2,4-dioxo-1,3-diazinane-5-carboximidamide Chemical compound CN1CC(C(N)=N)C(=O)NC1=O IXPNQXFRVYWDDI-UHFFFAOYSA-N 0.000 claims abstract description 9
- 244000235858 Acetobacter xylinum Species 0.000 claims abstract description 9
- 235000002837 Acetobacter xylinum Nutrition 0.000 claims abstract description 9
- 102000016943 Muramidase Human genes 0.000 claims abstract description 9
- 108010014251 Muramidase Proteins 0.000 claims abstract description 9
- 108010062010 N-Acetylmuramoyl-L-alanine Amidase Proteins 0.000 claims abstract description 9
- 229910021627 Tin(IV) chloride Inorganic materials 0.000 claims abstract description 9
- 239000012153 distilled water Substances 0.000 claims abstract description 9
- 229960000274 lysozyme Drugs 0.000 claims abstract description 9
- 235000010335 lysozyme Nutrition 0.000 claims abstract description 9
- 239000004325 lysozyme Substances 0.000 claims abstract description 9
- 239000000661 sodium alginate Substances 0.000 claims abstract description 9
- 235000010413 sodium alginate Nutrition 0.000 claims abstract description 9
- 229940005550 sodium alginate Drugs 0.000 claims abstract description 9
- HPGGPRDJHPYFRM-UHFFFAOYSA-J tin(iv) chloride Chemical compound Cl[Sn](Cl)(Cl)Cl HPGGPRDJHPYFRM-UHFFFAOYSA-J 0.000 claims abstract description 9
- 238000005406 washing Methods 0.000 claims abstract description 9
- 238000004227 thermal cracking Methods 0.000 claims abstract description 8
- 238000006243 chemical reaction Methods 0.000 claims abstract description 7
- 239000000047 product Substances 0.000 claims description 45
- 239000001963 growth medium Substances 0.000 claims description 25
- 238000009630 liquid culture Methods 0.000 claims description 25
- NLXLAEXVIDQMFP-UHFFFAOYSA-N Ammonia chloride Chemical compound [NH4+].[Cl-] NLXLAEXVIDQMFP-UHFFFAOYSA-N 0.000 claims description 12
- IAZDPXIOMUYVGZ-UHFFFAOYSA-N Dimethylsulphoxide Chemical compound CS(C)=O IAZDPXIOMUYVGZ-UHFFFAOYSA-N 0.000 claims description 12
- CSNNHWWHGAXBCP-UHFFFAOYSA-L Magnesium sulfate Chemical compound [Mg+2].[O-][S+2]([O-])([O-])[O-] CSNNHWWHGAXBCP-UHFFFAOYSA-L 0.000 claims description 12
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 claims description 12
- 238000005336 cracking Methods 0.000 claims description 12
- 239000012065 filter cake Substances 0.000 claims description 12
- JIAARYAFYJHUJI-UHFFFAOYSA-L zinc dichloride Chemical compound [Cl-].[Cl-].[Zn+2] JIAARYAFYJHUJI-UHFFFAOYSA-L 0.000 claims description 12
- 241000235070 Saccharomyces Species 0.000 claims description 10
- 238000000926 separation method Methods 0.000 claims description 8
- 229910021642 ultra pure water Inorganic materials 0.000 claims description 8
- 239000012498 ultrapure water Substances 0.000 claims description 8
- UXVMQQNJUSDDNG-UHFFFAOYSA-L Calcium chloride Chemical compound [Cl-].[Cl-].[Ca+2] UXVMQQNJUSDDNG-UHFFFAOYSA-L 0.000 claims description 6
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 claims description 6
- 229930003451 Vitamin B1 Natural products 0.000 claims description 6
- 235000019270 ammonium chloride Nutrition 0.000 claims description 6
- 239000001110 calcium chloride Substances 0.000 claims description 6
- 229910001628 calcium chloride Inorganic materials 0.000 claims description 6
- 239000008367 deionised water Substances 0.000 claims description 6
- 229910021641 deionized water Inorganic materials 0.000 claims description 6
- 239000011790 ferrous sulphate Substances 0.000 claims description 6
- 235000003891 ferrous sulphate Nutrition 0.000 claims description 6
- 239000008103 glucose Substances 0.000 claims description 6
- BAUYGSIQEAFULO-UHFFFAOYSA-L iron(2+) sulfate (anhydrous) Chemical compound [Fe+2].[O-]S([O-])(=O)=O BAUYGSIQEAFULO-UHFFFAOYSA-L 0.000 claims description 6
- 229910000359 iron(II) sulfate Inorganic materials 0.000 claims description 6
- 229910052943 magnesium sulfate Inorganic materials 0.000 claims description 6
- 235000019341 magnesium sulphate Nutrition 0.000 claims description 6
- 229940099596 manganese sulfate Drugs 0.000 claims description 6
- 239000011702 manganese sulphate Substances 0.000 claims description 6
- 235000007079 manganese sulphate Nutrition 0.000 claims description 6
- SQQMAOCOWKFBNP-UHFFFAOYSA-L manganese(II) sulfate Chemical compound [Mn+2].[O-]S([O-])(=O)=O SQQMAOCOWKFBNP-UHFFFAOYSA-L 0.000 claims description 6
- 229910000402 monopotassium phosphate Inorganic materials 0.000 claims description 6
- 235000019796 monopotassium phosphate Nutrition 0.000 claims description 6
- GNSKLFRGEWLPPA-UHFFFAOYSA-M potassium dihydrogen phosphate Chemical compound [K+].OP(O)([O-])=O GNSKLFRGEWLPPA-UHFFFAOYSA-M 0.000 claims description 6
- LWIHDJKSTIGBAC-UHFFFAOYSA-K potassium phosphate Substances [K+].[K+].[K+].[O-]P([O-])([O-])=O LWIHDJKSTIGBAC-UHFFFAOYSA-K 0.000 claims description 6
- 239000011780 sodium chloride Substances 0.000 claims description 6
- 229960003495 thiamine Drugs 0.000 claims description 6
- DPJRMOMPQZCRJU-UHFFFAOYSA-M thiamine hydrochloride Chemical compound Cl.[Cl-].CC1=C(CCO)SC=[N+]1CC1=CN=C(C)N=C1N DPJRMOMPQZCRJU-UHFFFAOYSA-M 0.000 claims description 6
- 239000011691 vitamin B1 Substances 0.000 claims description 6
- 235000010374 vitamin B1 Nutrition 0.000 claims description 6
- 239000011592 zinc chloride Substances 0.000 claims description 6
- 235000005074 zinc chloride Nutrition 0.000 claims description 6
- TWRXJAOTZQYOKJ-UHFFFAOYSA-L Magnesium chloride Chemical compound [Mg+2].[Cl-].[Cl-] TWRXJAOTZQYOKJ-UHFFFAOYSA-L 0.000 claims description 4
- IMNIMPAHZVJRPE-UHFFFAOYSA-N triethylenediamine Chemical compound C1CN2CCN1CC2 IMNIMPAHZVJRPE-UHFFFAOYSA-N 0.000 claims description 4
- 230000002194 synthesizing effect Effects 0.000 claims description 3
- 238000010438 heat treatment Methods 0.000 claims description 2
- 229910001629 magnesium chloride Inorganic materials 0.000 claims description 2
- 239000003960 organic solvent Substances 0.000 claims description 2
- 239000011241 protective layer Substances 0.000 claims description 2
- 230000008439 repair process Effects 0.000 claims description 2
- 239000007788 liquid Substances 0.000 claims 1
- 239000002609 medium Substances 0.000 claims 1
- 230000005855 radiation Effects 0.000 claims 1
- 239000002994 raw material Substances 0.000 abstract description 7
- 239000002537 cosmetic Substances 0.000 abstract description 5
- 238000004519 manufacturing process Methods 0.000 abstract description 5
- 230000000813 microbial effect Effects 0.000 abstract description 4
- 230000007760 free radical scavenging Effects 0.000 abstract description 3
- 235000014680 Saccharomyces cerevisiae Nutrition 0.000 description 37
- 241000235347 Schizosaccharomyces pombe Species 0.000 description 10
- 238000005457 optimization Methods 0.000 description 7
- 238000002360 preparation method Methods 0.000 description 7
- 230000000694 effects Effects 0.000 description 6
- 210000003491 skin Anatomy 0.000 description 5
- 230000000052 comparative effect Effects 0.000 description 4
- 150000003254 radicals Chemical class 0.000 description 4
- -1 oxygen Radicals Chemical class 0.000 description 3
- 229920001661 Chitosan Polymers 0.000 description 2
- HNDVDQJCIGZPNO-YFKPBYRVSA-N L-histidine Chemical compound OC(=O)[C@@H](N)CC1=CN=CN1 HNDVDQJCIGZPNO-YFKPBYRVSA-N 0.000 description 2
- 230000002292 Radical scavenging effect Effects 0.000 description 2
- 230000003078 antioxidant effect Effects 0.000 description 2
- 230000009286 beneficial effect Effects 0.000 description 2
- UCMIRNVEIXFBKS-UHFFFAOYSA-N beta-alanine Chemical compound NCCC(O)=O UCMIRNVEIXFBKS-UHFFFAOYSA-N 0.000 description 2
- 238000001514 detection method Methods 0.000 description 2
- TUJKJAMUKRIRHC-UHFFFAOYSA-N hydroxyl Chemical compound [OH] TUJKJAMUKRIRHC-UHFFFAOYSA-N 0.000 description 2
- 230000000968 intestinal effect Effects 0.000 description 2
- 238000012360 testing method Methods 0.000 description 2
- PHIQHXFUZVPYII-ZCFIWIBFSA-O (R)-carnitinium Chemical compound C[N+](C)(C)C[C@H](O)CC(O)=O PHIQHXFUZVPYII-ZCFIWIBFSA-O 0.000 description 1
- 206010000050 Abdominal adhesions Diseases 0.000 description 1
- 230000033616 DNA repair Effects 0.000 description 1
- 108010016626 Dipeptides Proteins 0.000 description 1
- 206010028980 Neoplasm Diseases 0.000 description 1
- 206010051246 Photodermatosis Diseases 0.000 description 1
- 238000010521 absorption reaction Methods 0.000 description 1
- 230000003213 activating effect Effects 0.000 description 1
- 239000013543 active substance Substances 0.000 description 1
- 230000032683 aging Effects 0.000 description 1
- 150000001299 aldehydes Chemical class 0.000 description 1
- 229940024606 amino acid Drugs 0.000 description 1
- 150000001413 amino acids Chemical class 0.000 description 1
- 239000003963 antioxidant agent Substances 0.000 description 1
- 229940000635 beta-alanine Drugs 0.000 description 1
- 210000005013 brain tissue Anatomy 0.000 description 1
- 229960004203 carnitine Drugs 0.000 description 1
- 210000000170 cell membrane Anatomy 0.000 description 1
- 210000002421 cell wall Anatomy 0.000 description 1
- 239000011248 coating agent Substances 0.000 description 1
- 238000000576 coating method Methods 0.000 description 1
- 230000001086 cytosolic effect Effects 0.000 description 1
- 230000006378 damage Effects 0.000 description 1
- 238000000354 decomposition reaction Methods 0.000 description 1
- 210000004207 dermis Anatomy 0.000 description 1
- 235000014113 dietary fatty acids Nutrition 0.000 description 1
- 230000001804 emulsifying effect Effects 0.000 description 1
- 239000000839 emulsion Substances 0.000 description 1
- 239000002158 endotoxin Substances 0.000 description 1
- 210000002615 epidermis Anatomy 0.000 description 1
- 239000000194 fatty acid Substances 0.000 description 1
- 229930195729 fatty acid Natural products 0.000 description 1
- 150000004665 fatty acids Chemical class 0.000 description 1
- 239000012634 fragment Substances 0.000 description 1
- 210000001035 gastrointestinal tract Anatomy 0.000 description 1
- 150000004676 glycans Chemical class 0.000 description 1
- 230000036541 health Effects 0.000 description 1
- 229960002885 histidine Drugs 0.000 description 1
- 229940088592 immunologic factor Drugs 0.000 description 1
- 239000000367 immunologic factor Substances 0.000 description 1
- 230000001506 immunosuppresive effect Effects 0.000 description 1
- 230000002779 inactivation Effects 0.000 description 1
- 239000010410 layer Substances 0.000 description 1
- 150000002632 lipids Chemical class 0.000 description 1
- 210000002540 macrophage Anatomy 0.000 description 1
- 230000004060 metabolic process Effects 0.000 description 1
- 239000002207 metabolite Substances 0.000 description 1
- 210000003205 muscle Anatomy 0.000 description 1
- 235000015097 nutrients Nutrition 0.000 description 1
- 238000007254 oxidation reaction Methods 0.000 description 1
- 230000036542 oxidative stress Effects 0.000 description 1
- 229910052760 oxygen Inorganic materials 0.000 description 1
- 239000001301 oxygen Substances 0.000 description 1
- 238000005502 peroxidation Methods 0.000 description 1
- 230000008845 photoaging Effects 0.000 description 1
- 229920001282 polysaccharide Polymers 0.000 description 1
- 239000005017 polysaccharide Substances 0.000 description 1
- 108090000765 processed proteins & peptides Proteins 0.000 description 1
- 230000001737 promoting effect Effects 0.000 description 1
- 239000007787 solid Substances 0.000 description 1
- 230000004936 stimulating effect Effects 0.000 description 1
- 230000037072 sun protection Effects 0.000 description 1
- 238000010998 test method Methods 0.000 description 1
- 239000011573 trace mineral Substances 0.000 description 1
- 235000013619 trace mineral Nutrition 0.000 description 1
- 230000002087 whitening effect Effects 0.000 description 1
Classifications
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
- A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
- A61K8/9728—Fungi, e.g. yeasts
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/64—Proteins; Peptides; Derivatives or degradation products thereof
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/72—Cosmetics or similar toiletry preparations characterised by the composition containing organic macromolecular compounds
- A61K8/73—Polysaccharides
- A61K8/731—Cellulose; Quaternized cellulose derivatives
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/92—Oils, fats or waxes; Derivatives thereof, e.g. hydrogenation products thereof
- A61K8/922—Oils, fats or waxes; Derivatives thereof, e.g. hydrogenation products thereof of vegetable origin
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q17/00—Barrier preparations; Preparations brought into direct contact with the skin for affording protection against external influences, e.g. sunlight, X-rays or other harmful rays, corrosive materials, bacteria or insect stings
- A61Q17/04—Topical preparations for affording protection against sunlight or other radiation; Topical sun tanning preparations
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
- A61Q19/08—Anti-ageing preparations
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P19/00—Preparation of compounds containing saccharide radicals
- C12P19/04—Polysaccharides, i.e. compounds containing more than five saccharide radicals attached to each other by glycosidic bonds
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P19/00—Preparation of compounds containing saccharide radicals
- C12P19/14—Preparation of compounds containing saccharide radicals produced by the action of a carbohydrase (EC 3.2.x), e.g. by alpha-amylase, e.g. by cellulase, hemicellulase
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P39/00—Processes involving microorganisms of different genera in the same process, simultaneously
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/40—Chemical, physico-chemical or functional or structural properties of particular ingredients
- A61K2800/59—Mixtures
- A61K2800/592—Mixtures of compounds complementing their respective functions
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/80—Process related aspects concerning the preparation of the cosmetic composition or the storage or application thereof
- A61K2800/85—Products or compounds obtained by fermentation, e.g. yoghurt, beer, wine
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- Genetics & Genomics (AREA)
- Dermatology (AREA)
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- General Engineering & Computer Science (AREA)
- General Chemical & Material Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Mycology (AREA)
- Oil, Petroleum & Natural Gas (AREA)
- Botany (AREA)
- Gerontology & Geriatric Medicine (AREA)
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- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
Abstract
The invention discloses a synthetic process of a schizosaccharomyces cerevisiae fermentation product lysate and carnosine composition, and relates to the technical field of microbial cosmetic raw material production. The method comprises the steps of fermenting acetobacter xylinum and yeast schizolysis to obtain mixed bacterial cellulose, treating the mixed bacterial cellulose with lysozyme, adding sodium alginate for thermal cracking to obtain modified mixed bacterial cellulose, reacting the mixed bacterial cellulose with epoxidized soybean oil under the action of stannic chloride to obtain a blank, mixing the blank and L-carnosine in distilled water, adding a catalyst, stirring for reaction, filtering, washing and drying to obtain the yeast schizolysis product and carnosine composition. The yeast schizosaccharomyces cerevisiae fermentation product lysate and carnosine composition prepared by the invention has excellent free radical scavenging capacity and can effectively protect the skin.
Description
Technical Field
The invention relates to the technical field of microbial cosmetic raw material production, in particular to a synthesis process and an effect of a schizosaccharomyces cerevisiae fermentation product lysate and carnosine composition.
Background
The yeast bifida is one of intestinal flora of human body, has strong intestinal adhesion and strong capability of being planted in the intestinal tract, and has important physiological health care functions of activating macrophages, stimulating the release of immune factors, maintaining the balance of the intestinal flora, resisting tumors, promoting the absorption of trace elements, controlling the generation of endotoxin and the like, so the yeast bifida has extremely high application value. The lysate of the yeast fermentation product is a natural active cosmetic raw material with wide application, and the raw material is subjected to strain culture, inactivation and decomposition to obtain a metabolite, a cytoplasmic fragment, a cell wall component and a polysaccharide complex; the raw material has strong immunosuppressive activity, can promote DNA repair, can effectively protect skin from being damaged by ultraviolet rays, is used for skin care, sun protection and after-sun care products of emulsion, water-based and hydroalcoholic systems, and helps to prevent photoaging of epidermis and dermis; in addition, the composition can strengthen the metabolism of the horny layer, can capture free radicals, inhibits the peroxidation of lipid, and has the functions of whitening and resisting aging; the product is rich in nutrients, and has skin nourishing effect.
In the prior art, due to the limitation of a production process, the free radical clearance rate of the produced microbial cosmetic raw material is greatly reduced, and an excessively violent production process can cause a large amount of active substances for clearing free radicals to lose activity, so that the antioxidant activity of the microbial cosmetic raw material is reduced, and the production cost is increased under the condition of the same activity.
Carnosine, known by the scientific name β -alanyl-L-histidine, is a crystalline solid composed of a dipeptide consisting of two amino acids, β -alanine and L-histidine. Muscle and brain tissues contain very high concentrations of carnosine. Carnosine is a peptide found by the russian chemist gule vicker together with carnitine. Studies in the uk, korea, russia and other countries have shown that: carnosine has strong antioxidant capacity and is beneficial to human body. Carnosine has been shown to scavenge reactive oxygen Radicals (ROS) and α - β unsaturated aldehydes, which form during oxidative stress over-oxidation of cell membrane fatty acids.
The invention combines the lysate of the fermentation product of the yeast schizosaccharomyces with the carnosine, so that the product has better free radical scavenging capacity.
Disclosure of Invention
The invention aims to provide a synthetic process and an effect of a schizosaccharomyces cerevisiae fermentation product lysate and carnosine composition, so as to solve the problems in the prior art.
A process for synthesizing a composition of a lysate and carnosine of a fermentation product of a yeast schizosaccharomyces, which mainly comprises the following steps:
(1) mixing acetobacter xylinum and saccharomyces bifidus in a liquid culture medium, performing mixed culture, and performing centrifugal separation to obtain mixed bacterial cellulose;
(2) re-dissolving the mixed bacterial cellulose obtained in the step (1) by using sterile ultrapure water, adding lysozyme, stirring for reaction, then adding sodium alginate, heating, stirring for reaction, and filtering to obtain modified mixed bacterial cellulose;
(3) mixing the modified mixed bacterial cellulose obtained in the step (2) with an organic solvent, adding epoxidized soybean oil, stirring and mixing, adding stannic chloride, stirring and reacting, filtering, and drying to obtain a blank;
(4) and (3) mixing the blank obtained in the step (3) and L-carnosine in distilled water, adding a catalyst, stirring for reaction, filtering, washing and drying to obtain the schizosaccharomyces cerevisiae fermentation product lysate and carnosine composition.
As optimization, the two split yeast fermentation product lysate and carnosine composition synthesis process mainly includes the following preparation steps:
(1) mixing acetobacter xylinum and saccharomyces bifidus in a liquid culture medium with the mass ratio of 1: 1-1: 2 being 50-100 times of that of the saccharomyces bifidus, performing mixed culture at the temperature of 30-38 ℃ until the pH of the liquid culture medium is less than 4.0, performing centrifugal separation at the rotation speed of 3000-3500 r/min for 10min, and filtering to obtain mixed bacterial cellulose;
(2) mixing and re-dissolving the mixed bacterial cellulose obtained in the step (1) and sterile ultrapure water in a beaker according to a mass ratio of 1: 50-1: 80, adding lysozyme with the mass of 0.1-0.2 times of that of the mixed bacterial cellulose into the beaker, stirring and reacting for 10-30 min at the temperature of 30-40 ℃ and the rotating speed of 200-400 r/min, adding sodium alginate with the mass of 0.2-0.4 times of that of the mixed bacterial cellulose into the beaker, thermally cracking for 15-20 min, and filtering to obtain modified mixed bacterial cellulose;
(3) mixing the modified mixed bacterial cellulose obtained in the step (2) and dimethyl sulfoxide in a mass ratio of 1: 15-1: 20 in a flask, adding epoxidized soybean oil with the mass being 0.6-1.2 times that of the modified mixed bacterial cellulose into the flask, stirring and mixing for 10-30 min at the temperature of 40-60 ℃ and the rotating speed of 250-300 r/min, adding stannic chloride with the mass being 0.1-0.2 times that of the modified mixed bacterial cellulose into the flask, stirring and reacting for 100-120 min at the temperature of 60-70 ℃ and the rotating speed of 300-350 r/min, filtering to obtain a filter cake, and drying the filter cake for 2-3 h at the temperature of 50-70 ℃ to obtain a blank;
(4) mixing the blank obtained in the step (3) and L-carnosine in distilled water with the mass ratio of 2: 1-6: 1 of 8-15 times of the mass of the blank, adding a catalyst with the mass of 0.1-0.2 times of the mass of the blank, stirring and reacting for 2-6 hours at the temperature of 55-75 ℃, filtering to obtain a pretreated blank, washing the pretreated blank with deionized water for 2-5 times, and drying for 1-3 hours at the temperature of 60 ℃ to obtain a yeast bifida fermentation product lysate and a carnosine composition.
As optimization, the preparation method of the liquid culture medium in the step (1) comprises the following steps: 10g of glucose, 2g of ammonium chloride, 2g of monopotassium phosphate, 0.1g of calcium chloride, 0.25g of magnesium sulfate, 0.1g of ferrous sulfate, 1mg of sodium chloride, 0.5mg of manganese sulfate and 5mg of vitamin B1 are weighed in sequence and mixed in 1L of water, and the mixture is stirred and mixed to obtain a liquid culture medium.
And (3) optimally, the thermal cracking temperature in the step (2) is 80-100 ℃.
Preferably, the catalyst in the step (4) is any one of zinc chloride, magnesium chloride or triethylene diamine.
As optimization, the yeast fermentation lysate and carnosine composition prepared by the synthesis process of the yeast fermentation lysate and carnosine composition can be applied to skin repair, and can be used as a protective layer of skin to isolate ultraviolet rays.
Compared with the prior art, the invention has the beneficial effects that:
according to the invention, epoxidized soybean oil is added in the preparation of the schizosaccharomyces cerevisiae fermentation product lysate and carnosine composition, and bacterial cellulose is used for fixing the schizosaccharomyces cerevisiae fermentation product lysate.
Firstly, acetobacter xylinum is added when a lysate of a fermentation product of the saccharomyces cerevisiae is prepared, and can be fermented at one time when the saccharomyces cerevisiae is fermented, so that a fermentation system is rapidly acidified, the fermentation of the saccharomyces cerevisiae is promoted, and meanwhile, bacterial cellulose formed by the fermentation of the acetobacter xylinum can coat the fermentation product of the saccharomyces cerevisiae in the fermentation process of the saccharomyces cerevisiae, so that the excessive damage of the fermentation product of the saccharomyces cerevisiae during thermal cracking is reduced, and the free radical scavenging capability of the product is ensured;
secondly, epoxidized soybean oil is used for connecting the lysate of the yeast fermentation product with carnosine when the composition of the lysate of the yeast fermentation product and the carnosine is prepared, so that the free radical removing capacity is improved, the epoxidized soybean oil is added in the connecting process, so that the product has lipophilicity, and the accessed modified mixed bacterial cellulose and the L-carnosine have good water solubility, so that the emulsifying property of the product is endowed.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
To more clearly illustrate the method of the present invention is illustrated in detail by the following examples, in which the following test methods for the respective indices of the split yeast fermentation lysate and carnosine composition are made as follows:
radical scavenging ability: mixing the cracked yeast fermentation product lysate and carnosine composition obtained in each example with a comparative example product and water according to a mass ratio of 1:5, and testing DPPH free radical clearance rate by respectively using DPPH free radical detection kits; and (3) testing the hydroxyl radical clearance rate by using a hydroxyl radical detection kit.
Example 1
A process for the synthesis of a split yeast fermentation lysate and carnosine composition, said process essentially comprising the steps of:
(1) mixing acetobacter xylinum and saccharomyces bifidus in a liquid culture medium with the mass ratio of 1:2 being 60 times that of the saccharomyces bifidus, performing mixed culture at the temperature of 36 ℃ until the pH value of the liquid culture medium is less than 4.0, performing centrifugal separation for 10min at the rotation speed of 3000r/min, and filtering to obtain mixed bacterial cellulose;
(2) mixing and re-dissolving the mixed bacterial cellulose obtained in the step (1) and sterile ultrapure water in a beaker according to a mass ratio of 1:60, adding lysozyme with the mass of 0.15 times that of the mixed bacterial cellulose into the beaker, stirring and reacting for 15min at the temperature of 35 ℃ and the rotating speed of 260r/min, adding sodium alginate with the mass of 0.3 time that of the mixed bacterial cellulose into the beaker, thermally cracking for 18min, and filtering to obtain modified mixed bacterial cellulose;
(3) mixing the modified mixed bacterial cellulose obtained in the step (2) and dimethyl sulfoxide in a mass ratio of 1:18 in a flask, adding epoxidized soybean oil with the mass being 0.8 time that of the modified mixed bacterial cellulose into the flask, stirring and mixing for 20min at the temperature of 50 ℃ and the rotating speed of 280r/min, adding stannic chloride with the mass being 0.15 time that of the modified mixed bacterial cellulose into the flask, stirring and reacting for 100min at the temperature of 68 ℃ and the rotating speed of 320r/min, filtering to obtain a filter cake, and drying the filter cake for 3h at the temperature of 60 ℃ to obtain a blank;
(4) mixing the blank obtained in the step (3) and L-carnosine in distilled water with the mass ratio of 3:1 being 10 times of that of the blank, adding a catalyst with the mass being 0.15 times of that of the blank, stirring and reacting for 3 hours at the temperature of 70 ℃, filtering to obtain a pretreated blank, washing the pretreated blank with deionized water for 2-5 times, and drying for 2 hours at the temperature of 60 ℃ to obtain the cracked yeast fermentation product lysate and the carnosine composition.
As optimization, the preparation method of the liquid culture medium in the step (1) comprises the following steps: 10g of glucose, 2g of ammonium chloride, 2g of monopotassium phosphate, 0.1g of calcium chloride, 0.25g of magnesium sulfate, 0.1g of ferrous sulfate, 1mg of sodium chloride, 0.5mg of manganese sulfate and 5mg of vitamin B1 are weighed in sequence and mixed in 1L of water, and the mixture is stirred and mixed to obtain a liquid culture medium.
Preferably, the thermal cracking temperature in the step (2) is 85 ℃.
Preferably, the catalyst in the step (4) is zinc chloride.
Example 2
A process for the synthesis of a split yeast fermentation lysate and carnosine composition, said process essentially comprising the steps of:
(1) mixing the secondary fission yeast in a liquid culture medium with the mass ratio of 1:2 being 60 times of that of the secondary fission yeast, carrying out mixed culture at the temperature of 36 ℃ until the pH value of the liquid culture medium is less than 4.0, carrying out centrifugal separation for 10min at the rotation speed of 3000r/min, and filtering to obtain a secondary fission yeast fermentation product;
(2) mixing and re-dissolving the secondary fission yeast fermentation product obtained in the step (1) and sterile ultrapure water according to the mass ratio of 1:60 in a beaker, adding lysozyme with the mass of 0.15 time that of the mixed bacterial cellulose into the beaker, stirring and reacting for 15min at the temperature of 35 ℃ and the rotating speed of 260r/min, adding sodium alginate with the mass of 0.3 time that of the mixed bacterial cellulose into the beaker, thermally cracking for 18min, and filtering to obtain a modified secondary fission yeast fermentation product;
(3) mixing the modified secondary cracking yeast fermentation product obtained in the step (2) and dimethyl sulfoxide in a mass ratio of 1:18 in a flask, adding epoxidized soybean oil with the mass of 0.8 time that of the modified secondary cracking yeast fermentation product into the flask, stirring and mixing for 20min at the temperature of 50 ℃ and the rotating speed of 280r/min, adding tin tetrachloride with the mass of 0.15 time that of the modified secondary cracking yeast fermentation product into the flask, stirring and reacting for 100min at the temperature of 68 ℃ and the rotating speed of 320r/min, filtering to obtain a filter cake, and drying the filter cake for 3h at the temperature of 60 ℃ to obtain a blank;
(4) mixing the blank obtained in the step (3) and L-carnosine in distilled water with the mass ratio of 3:1 being 10 times of that of the blank, adding a catalyst with the mass being 0.15 times of that of the blank, stirring and reacting for 3 hours at the temperature of 70 ℃, filtering to obtain a pretreated blank, washing the pretreated blank with deionized water for 2-5 times, and drying for 2 hours at the temperature of 60 ℃ to obtain the cracked yeast fermentation product lysate and the carnosine composition.
As optimization, the preparation method of the liquid culture medium in the step (1) comprises the following steps: 10g of glucose, 2g of ammonium chloride, 2g of monopotassium phosphate, 0.1g of calcium chloride, 0.25g of magnesium sulfate, 0.1g of ferrous sulfate, 1mg of sodium chloride, 0.5mg of manganese sulfate and 5mg of vitamin B1 are weighed in sequence and mixed in 1L of water, and the mixture is stirred and mixed to obtain a liquid culture medium.
Preferably, the thermal cracking temperature in the step (2) is 85 ℃.
Preferably, the catalyst in the step (4) is zinc chloride.
Example 3
A process for the synthesis of a split yeast fermentation lysate and carnosine composition, said process essentially comprising the steps of:
(1) mixing acetobacter xylinum and saccharomyces bifidus in a liquid culture medium with the mass ratio of 1:2 being 60 times that of the saccharomyces bifidus, performing mixed culture at the temperature of 36 ℃ until the pH value of the liquid culture medium is less than 4.0, performing centrifugal separation for 10min at the rotation speed of 3000r/min, and filtering to obtain mixed bacterial cellulose;
(2) mixing and re-dissolving the mixed bacterial cellulose obtained in the step (1) and sterile ultrapure water in a beaker according to a mass ratio of 1:60, adding lysozyme with the mass of 0.15 times that of the mixed bacterial cellulose into the beaker, stirring and reacting for 15min at the temperature of 35 ℃ and the rotating speed of 260r/min, adding sodium alginate with the mass of 0.3 time that of the mixed bacterial cellulose into the beaker, thermally cracking for 18min, and filtering to obtain modified mixed bacterial cellulose;
(3) mixing the modified mixed bacterial cellulose obtained in the step (2) and dimethyl sulfoxide in a mass ratio of 1:18 in a flask, adding chitosan with the mass being 0.8 time that of the modified mixed bacterial cellulose into the flask, stirring and mixing for 20min at the temperature of 50 ℃ and the rotating speed of 280r/min, adding stannic chloride with the mass being 0.15 time that of the modified mixed bacterial cellulose into the flask, stirring and reacting for 100min at the temperature of 68 ℃ and the rotating speed of 320r/min, filtering to obtain a filter cake, and drying the filter cake for 3h at the temperature of 60 ℃ to obtain a blank;
(4) mixing the blank obtained in the step (3) and L-carnosine in distilled water with the mass ratio of 3:1 being 10 times of that of the blank, adding a catalyst with the mass being 0.15 times of that of the blank, stirring and reacting for 3 hours at the temperature of 70 ℃, filtering to obtain a pretreated blank, washing the pretreated blank with deionized water for 2-5 times, and drying for 2 hours at the temperature of 60 ℃ to obtain the cracked yeast fermentation product lysate and the carnosine composition.
As optimization, the preparation method of the liquid culture medium in the step (1) comprises the following steps: 10g of glucose, 2g of ammonium chloride, 2g of monopotassium phosphate, 0.1g of calcium chloride, 0.25g of magnesium sulfate, 0.1g of ferrous sulfate, 1mg of sodium chloride, 0.5mg of manganese sulfate and 5mg of vitamin B1 are weighed in sequence and mixed in 1L of water, and the mixture is stirred and mixed to obtain a liquid culture medium.
Preferably, the thermal cracking temperature in the step (2) is 85 ℃.
Preferably, the catalyst in the step (4) is zinc chloride.
Comparative example
A process for the synthesis of a split yeast fermentation lysate and carnosine composition, said process essentially comprising the steps of:
(1) mixing the secondary fission yeast in a liquid culture medium with the mass ratio of 1:2 being 60 times of that of the secondary fission yeast, carrying out mixed culture at the temperature of 36 ℃ until the pH value of the liquid culture medium is less than 4.0, carrying out centrifugal separation for 10min at the rotation speed of 3000r/min, and filtering to obtain a secondary fission yeast fermentation product;
(2) mixing and re-dissolving the secondary fission yeast fermentation product obtained in the step (1) and sterile ultrapure water according to the mass ratio of 1:60 in a beaker, adding lysozyme with the mass of 0.15 time that of the mixed bacterial cellulose into the beaker, stirring and reacting for 15min at the temperature of 35 ℃ and the rotating speed of 260r/min, adding sodium alginate with the mass of 0.3 time that of the mixed bacterial cellulose into the beaker, thermally cracking for 18min, and filtering to obtain a modified secondary fission yeast fermentation product;
(3) mixing the modified secondary cracking yeast fermentation product obtained in the step (2) and dimethyl sulfoxide in a mass ratio of 1:18 in a flask, adding chitosan with the mass of 0.8 time that of the modified secondary cracking yeast fermentation product into the flask, stirring and mixing for 20min at the temperature of 50 ℃ and the rotating speed of 280r/min, adding tin tetrachloride with the mass of 0.15 time that of the modified secondary cracking yeast fermentation product into the flask, stirring and reacting for 100min at the temperature of 68 ℃ and the rotating speed of 320r/min, filtering to obtain a filter cake, and drying the filter cake for 3h at the temperature of 60 ℃ to obtain a blank;
(4) mixing the blank obtained in the step (3) and L-carnosine in distilled water with the mass ratio of 3:1 being 10 times of that of the blank, adding a catalyst with the mass being 0.15 times of that of the blank, stirring and reacting for 3 hours at the temperature of 70 ℃, filtering to obtain a pretreated blank, washing the pretreated blank with deionized water for 2-5 times, and drying for 2 hours at the temperature of 60 ℃ to obtain the cracked yeast fermentation product lysate and the carnosine composition.
As optimization, the preparation method of the liquid culture medium in the step (1) comprises the following steps: 10g of glucose, 2g of ammonium chloride, 2g of monopotassium phosphate, 0.1g of calcium chloride, 0.25g of magnesium sulfate, 0.1g of ferrous sulfate, 1mg of sodium chloride, 0.5mg of manganese sulfate and 5mg of vitamin B1 are weighed in sequence and mixed in 1L of water, and the mixture is stirred and mixed to obtain a liquid culture medium.
Preferably, the thermal cracking temperature in the step (2) is 85 ℃.
Preferably, the catalyst in the step (4) is zinc chloride.
Examples of effects
The following table 1 shows the results of performance analysis of the split yeast fermentation product lysates and carnosine compositions prepared using examples 1 to 3 of the present invention and comparative examples.
TABLE 1
From the comparison of the experimental data of example 1 and the comparative example in table 1, it can be found that the radical scavenging ability of the product can be effectively improved by coating the split yeast fermentation lysate with bacterial cellulose and connecting the split yeast fermentation lysate to carnosine with epoxidized soybean oil in the split yeast fermentation lysate and carnosine composition.
It will be evident to those skilled in the art that the invention is not limited to the details of the foregoing illustrative embodiments, and that the present invention may be embodied in other specific forms without departing from the spirit or essential attributes thereof. The present embodiments are therefore to be considered in all respects as illustrative and not restrictive, the scope of the invention being indicated by the appended claims rather than by the foregoing description, and all changes which come within the meaning and range of equivalency of the claims are therefore intended to be embraced therein. Any reference sign in a claim should not be construed as limiting the claim concerned.
Claims (6)
1. A process for synthesizing a composition of a lysate and carnosine of a fermentation product of a yeast schizosaccharomyces, which mainly comprises the following steps:
(1) mixing acetobacter xylinum and saccharomyces bifidus in a liquid culture medium, performing mixed culture, and performing centrifugal separation to obtain mixed bacterial cellulose;
(2) re-dissolving the mixed bacterial cellulose obtained in the step (1) by using sterile ultrapure water, adding lysozyme, stirring for reaction, then adding sodium alginate, heating, stirring for reaction, and filtering to obtain modified mixed bacterial cellulose;
(3) mixing the modified mixed bacterial cellulose obtained in the step (2) with an organic solvent, adding epoxidized soybean oil, stirring and mixing, adding stannic chloride, stirring and reacting, filtering, and drying to obtain a blank;
(4) and (3) mixing the blank obtained in the step (3) and L-carnosine in distilled water, adding a catalyst, stirring for reaction, filtering, washing and drying to obtain the schizosaccharomyces cerevisiae fermentation product lysate and carnosine composition.
2. The process of claim 1, wherein said process of synthesizing a split yeast fermentation lysate and carnosine composition comprises the steps of:
(1) mixing acetobacter xylinum and saccharomyces bifidus in a liquid culture medium with the mass ratio of 1: 1-1: 2 being 50-100 times of that of the saccharomyces bifidus, performing mixed culture at the temperature of 30-38 ℃ until the pH of the liquid culture medium is less than 4.0, performing centrifugal separation at the rotation speed of 3000-3500 r/min for 10min, and filtering to obtain mixed bacterial cellulose;
(2) mixing and re-dissolving the mixed bacterial cellulose obtained in the step (1) and sterile ultrapure water in a beaker according to a mass ratio of 1: 50-1: 80, adding lysozyme with the mass of 0.1-0.2 times of that of the mixed bacterial cellulose into the beaker, stirring and reacting for 10-30 min at the temperature of 30-40 ℃ and the rotating speed of 200-400 r/min, adding sodium alginate with the mass of 0.2-0.4 times of that of the mixed bacterial cellulose into the beaker, thermally cracking for 15-20 min, and filtering to obtain modified mixed bacterial cellulose;
(3) mixing the modified mixed bacterial cellulose obtained in the step (2) and dimethyl sulfoxide in a mass ratio of 1: 15-1: 20 in a flask, adding epoxidized soybean oil with the mass being 0.6-1.2 times that of the modified mixed bacterial cellulose into the flask, stirring and mixing for 10-30 min at the temperature of 40-60 ℃ and the rotating speed of 250-300 r/min, adding stannic chloride with the mass being 0.1-0.2 times that of the modified mixed bacterial cellulose into the flask, stirring and reacting for 100-120 min at the temperature of 60-70 ℃ and the rotating speed of 300-350 r/min, filtering to obtain a filter cake, and drying the filter cake for 2-3 h at the temperature of 50-70 ℃ to obtain a blank;
(4) mixing the blank obtained in the step (3) and L-carnosine in distilled water with the mass ratio of 2: 1-6: 1 of 8-15 times of the mass of the blank, adding a catalyst with the mass of 0.1-0.2 times of the mass of the blank, stirring and reacting for 2-6 hours at the temperature of 55-75 ℃, filtering to obtain a pretreated blank, washing the pretreated blank with deionized water for 2-5 times, and drying for 1-3 hours at the temperature of 60 ℃ to obtain a yeast bifida fermentation product lysate and a carnosine composition.
3. The process of claim 2, wherein the liquid medium of step (1) is prepared by the steps of: 10g of glucose, 2g of ammonium chloride, 2g of monopotassium phosphate, 0.1g of calcium chloride, 0.25g of magnesium sulfate, 0.1g of ferrous sulfate, 1mg of sodium chloride, 0.5mg of manganese sulfate and 5mg of vitamin B1 are weighed in sequence and mixed in 1L of water, and the mixture is stirred and mixed to obtain a liquid culture medium.
4. The process of claim 2, wherein the thermal cracking temperature of step (2) is 80-100 ℃.
5. The process of claim 2, wherein the catalyst in step (4) is any one of zinc chloride, magnesium chloride or triethylenediamine.
6. The process of claim 2, wherein the process produces a split yeast fermentation lysate and carnosine composition that can be used for skin repair and at the same time as a protective layer for the skin to protect against UV radiation.
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